SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011766501.1 NCBI__GCF_000061505.1:WP_011766501.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
69% identity, 100% coverage: 2:306/306 of query aligns to 1:306/306 of 4whxA

query
sites
4whxA

S

S

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M

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I

active site: F37 (= F38), K160 (= K160), E194 (= E194), L217 (= L217)
binding alanine: Y97 (= Y97), K160 (= K160), A258 (= A258)

2ej0B Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase with pyridoxamine 5'-phosphate
55% identity, 98% coverage: 6:306/306 of query aligns to 3:304/305 of 2ej0B

query
sites
2ej0B

R

K

D

A

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active site: F35 (= F38), G37 (= G40), K158 (= K160), E192 (= E194), L215 (= L217)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R58 (= R61), Y163 (= Y165), E192 (= E194), G195 (= G197), E196 (= E198), L215 (= L217), G217 (= G219), I218 (= I220), T219 (= T221), G254 (= G256), T255 (= T257)

2ej3A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with gabapentin
53% identity, 98% coverage: 6:306/306 of query aligns to 3:296/297 of 2ej3A

query
sites
2ej3A

R

K

D

A

G

G

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Y

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A

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A

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F

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A
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A

A

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M

L

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active site: F35 (= F38), G37 (= G40), K150 (= K160), E184 (= E194), L207 (= L217)
binding [1-(aminomethyl)cyclohexyl]acetic acid: G187 (= G197), G246 (= G256), T247 (= T257), A248 (= A258)
binding pyridoxal-5'-phosphate: R58 (= R61), K150 (= K160), Y155 (= Y165), E184 (= E194), G187 (= G197), L207 (= L217), G209 (= G219), I210 (= I220), T211 (= T221), G246 (= G256), T247 (= T257)

2eiyA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with 4-methylvaleric acid
53% identity, 98% coverage: 6:306/306 of query aligns to 3:296/297 of 2eiyA

query
sites
2eiyA

R

K

D

A

G

G

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E

A

A

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K

T

T

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V

L

L

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S

H

H

S

A

L

L

H

H

Y

Y

G

G

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S

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V

F
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F

E

E

G
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G

V

I

R

R

A

A

Y

Y

K

E

T

T

A

A

K

K

G

G

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P

A

A

I

I

F

F

R

R

L

L

A

K

E

E

H

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K

R
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L

F

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Y

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A

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I

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M

M

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A

A

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K

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A

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A
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A

A

A

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E

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P

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M

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R

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F

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V

active site: F35 (= F38), G37 (= G40), K150 (= K160), E184 (= E194), L207 (= L217)
binding 4-methyl valeric acid: F35 (= F38), Y94 (= Y97), T247 (= T257), A248 (= A258)
binding pyridoxal-5'-phosphate: R58 (= R61), K150 (= K160), Y155 (= Y165), E184 (= E194), G187 (= G197), E188 (= E198), L207 (= L217), G209 (= G219), I210 (= I220), T211 (= T221), G246 (= G256), T247 (= T257)

1wrvA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase
53% identity, 98% coverage: 6:306/306 of query aligns to 3:296/297 of 1wrvA

query
sites
1wrvA

R

K

D

A

G

G

F

L

I

I

W

W

Y

M

D

N

G

G

K

A

L

F

V

V

P

P

W

Q

R

E

D

E

A

A

T

K

T

T

H

S

V

V

L

L

T

S

H

H

S

A

L

L

H

H

Y

Y

G

G

L

T

S

S

V

V

F
|
F

E

E

G
|
G

V

I

R

R

A

A

Y

Y

K

E

T

T

A

A

K

K

G

G

T

P

A

A

I

I

F

F

R

R

L

L

A

K

E

E

H

H

T

V

Q

K

R
|
R

L

F

L

Y

N

N

S

S

G

A

K

K

I

V

Y

L

M

R

M

M

D

E

I

I

P

P

Y

F

S

A

K

P

E

E

V

E

L

L

M

E

E

E

A

A

Q

I

K

K

E

E

V

V

R

R

A

R

N

N

K

G

L

Y

E

R

S

S

C

C

Y

Y

L

I

R

R

P

P

I

L

A

A

F

W

Y

M

G

G

S

A

E

K

K

A

M

L

G

G

I

V

S

N

T

P

-

L

R

P

G

N

A

N

T

P

V

A

H

E

V

V

A

M

I

V

A

A

W

W

P

E

W

W

G

-

A

-

Y

-

L

-

G

-

E

-

D

-

G

-

L

V

E

R

K

K

G

G

I

A

R

R

V

L

K

I

T

T

S

S

F

W

A

A

R

R

Q

F

H

P

V

A

N

N

V

V

T

M

M

P

A

G

R

K

A

A

K
|
K

L

V

A

G

S

G

T

N

Y
|
Y

A

V

N

N

S

S

I

A

L

L

A

A

N

K

L

M

E

E

A

A

T

V

Q

A

D

A

G

G

Y

A

D

D

E

E

A

A

L

L

D

D

T

E

Q

E

G

G

F

Y

V

V

A

A

E
|
E

G

G

S

S

G
|
G

E

E

N

N

L

L

F

F

V

F

I

V

K

R

D

D

G

G

V

V

I

I

Y

Y

E

A

P

L

E

E

I

H

A

S

S

V

A

N

L
|
L

T

E

G
|
G

I
|
I

T
|
T

R

R

D

D

S

S

V

V

I

I

R

R

V

I

A

A

R

K

E

D

L

L

G

G

Y

Y

E

E

V

V

Q

Q

S

V

R

V

R

R

M

A

T

T

R

R

D

D

D

Q

I

L

Y

Y

I

M

A

A

D

D

E

E

A

V

F

F

F

M

T

T

G

G

T
|
T

A

A

E

E

V

V

T

T

P

P

I

V

R

S

E

M

L

I

D

D

N

W

R

R

T

P

I

I

G

G

E

K

G

G

R

T

R

A

G

G

P

P

I

V

T

A

T

L

K

R

V

L

Q

R

A

E

R

V

F

Y

F

L

D

E

I

A

V

V

N

T

G

G

R

R

A

R

P

P

E

E

Y

Y

E

E

N

G

W

W

L

L

S

T

L

Y

V

V

active site: F35 (= F38), G37 (= G40), K150 (= K160), E184 (= E194), L207 (= L217)
binding pyridoxal-5'-phosphate: R58 (= R61), K150 (= K160), Y155 (= Y165), E184 (= E194), G187 (= G197), L207 (= L217), G209 (= G219), I210 (= I220), T211 (= T221), T247 (= T257)

2ej2A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with n-(5'-phosphopyridoxyl)-l-glutamate
53% identity, 98% coverage: 6:306/306 of query aligns to 3:293/294 of 2ej2A

query
sites
2ej2A

R

K

D

A

G

G

F

L

I

I

W

W

Y

M

D

N

G

G

K

A

L

F

V

V

P

P

W

Q

R

E

D

E

A

A

T

K

T

T

H

S

V

V

L

L

T

S

H

H

S

A

L

L

H

H

Y

Y

G

G

L

T

S

S

V

V

F
|
F

E

E

G
|
G

V

I

R

R

A

A

Y

Y

K

E

T

T

A

A

K

K

G

G

T

P

A

A

I

I

F

F

R

R

L

L

A

K

E

E

H

H

T

V

Q

K

R
|
R

L

F

L

Y

N

N

S

S

G

A

K

K

I

V

Y

L

M

R

M

M

D

E

I

I

P

P

Y

F

S

A

K

P

E

E

V

E

L

L

M

E

E

E

A

A

Q

I

K

K

E

E

V

V

R

R

A

R

N

N

K

G

L

Y

E

R

S

S

C

C

Y
|
Y

L

I

R

R

P

P

I

L

A

A

F

W

Y

M

G

G

S

A

E

K

K

A

M

L

G

G

I

V

S

N

T

P

-

L

R

P

G

N

A

N

T

P

V

A

H

E

V

V

A

M

I

V

A

A

W

W

P

E

W

W

G

G

A

A

Y

-

L

-

G

-

E

-

D

-

G

-

L

-

E

-

K

-

G

-

I

-

R

R

V

L

K

I

T

T

S

S

F

W

A

A

R

R

Q

F

H

P

V

A

N

N

V

V

T

M

M

P

A

G

R

K

A

A

K
|
K

L

V

A

G

S

G

T

N

Y
|
Y

A

V

N

N

S

S

I

A

L

L

A

A

N

K

L

M

E

E

A

A

T

V

Q

A

D

A

G

G

Y

A

D

D

E

E

A

A

L

L

D

D

T

E

Q

E

G

G

F

Y

V

V

A

A

E
|
E

G

G

S

S

G
|
G

E
|
E

N

N

L

L

F

F

V

F

I

V

K

R

D

D

G

G

V

V

I

I

Y

Y

E

A

P

L

E

E

I

H

A

S

S

V

A

N

L
|
L

T

E

G
|
G

I
|
I

T
|
T

R

R

D

D

S

S

V

V

I

I

R

R

V

I

A

A

R

K

E

D

L

L

G

G

Y

Y

E

E

V

V

Q

Q

S

V

R

V

R

R

M

A

T

T

R

R

D

D

D

Q

I

L

Y

Y

I

M

A

A

D

D

E

E

A

V

F

F

F

M

T

T

G

G

T
|
T

A
|
A

A

A

E

E

V

V

T

T

P

P

I

V

R

S

E

M

L

I

D

D

N

W

R

R

T

P

I

I

G

G

E

K

G

G

R

T

R

A

G

G

P

P

I

V

T

A

T

L

K

R

V

L

Q

R

A

E

R

V

F

Y

F

L

D

E

I

A

V

V

N

T

G

G

R

R

A

R

P

P

E

E

Y

Y

E

E

N

G

W

W

L

L

S

T

L

Y

V

V

active site: F35 (= F38), G37 (= G40), K147 (= K160), E181 (= E194), L204 (= L217)
binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: R58 (= R61), Y94 (= Y97), Y152 (= Y165), E181 (= E194), G184 (= G197), E185 (= E198), L204 (= L217), G206 (= G219), I207 (= I220), T208 (= T221), T244 (= T257), A245 (= A258)

1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
47% identity, 97% coverage: 9:306/306 of query aligns to 4:303/304 of 1iyeA

query
sites
1iyeA

F

Y

I

I

W

W

Y

F

D

N

G

G

K

E

L

M

V

V

P

R

W

W

R

E

D

D

A

A

T

K

T

V

H

H

V

V

L

M

T

S

H

H

S

A

L

L

H

H

Y

Y

G

G

L

T

S

S

V

V

F
|
F

E

E

G
|
G

V

I

R

R

A

C

Y

Y

K

D

T

S

A

H

K

K

G

G

T

P

A

V

I

V

F

F

R

R

L

H

A

R

E

E

H

H

T

M

Q

Q

R
|
R

L

L

L

H

N

D

S

S

G

A

K

K

I

I

Y

Y

M

R

M

F

D

P

I

V

P

S

Y

Q

S

S

K

I

E

D

V

E

L

L

M

M

E

E

A

A

Q

C

K

R

E

D

V

V

V

I

R

R

A

K

N

N

K

N

L

L

E

T

S

S

C

A

Y
|
Y

L

I

R

R

P

P

I

L

A

I

F

F

Y

V

G

G

S

D

E

V

K

G

M

M

G

G

I

V

S

N

T

P

R

P

-

A

G

G

A

Y

T

S

V

T

H

D

V

V

A

I

I

I

A

A

W

F

P

P

W

W

G

G

A

A

Y
|
Y

L

L

G

G

E

A

D

E

G

A

L

L

E

E

K

Q

G

G

I

I

R

D

V

A

K

M

T

V

S

S

F

W

A

N

R

R

Q

A

H

A

V

P

N

N

V

T

T

I

M

P

A

T

R

A

A

A

K
|
K

L
x
A

A

G

S

G

T

N

Y
|
Y

A

L

N

S

S

S

I

L

L

L

A

V

N

G

L

S

E

E

A

A

T

R

Q

R

D

H

G

G

Y

Y

D

Q

E

E

A

G

L

I

L

A

L

L

D

D

T

V

Q

N

G

G

F

Y

V

I

A

S

E
|
E

G

G

S

A

G
|
G

E
|
E

N
|
N

L

L

F

F

V

E

I

V

K

K

D

D

G

G

V

V

I

L

Y

F

E

T

P

P

E

P

I

F

-

T

A

S

S

S

A

A

L
|
L

T

P

G
|
G

I
|
I

T
|
T

R

R

D

D

S

A

V

I

I

I

R

K

V

L

A

A

R

K

E

E

L

L

G

G

Y

I

E

E

V

V

Q

R

S

E

R

Q

R

V

M

L

T

S

R

R

D

E

D

S

I

L

Y

Y

I

L

A

A

D

D

E

E

A

V

F

F

F

M

T

S

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

T

T

P

P

I

V

R

R

E

S

L

V

D

D

N

G

R

I

T

Q

I

V

G

G

E

E

G

G

R

R

R

C

G

G

P

P

I

V

T

T

T

K

K

R

V

I

Q

Q

A

Q

R

A

F

F

D

G

I

L

V

F

N

T

G

G

R

E

A

T

P

E

E

D

Y

K

E

W

N

G

W

W

L

L

S

D

L

Q

V

V

active site: F33 (= F38), G35 (= G40), K156 (= K160), A157 (≠ L161), E190 (= E194), L214 (= L217)
binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R61), Y92 (= Y97), Y126 (= Y130), K156 (= K160), Y161 (= Y165), E190 (= E194), G193 (= G197), E194 (= E198), N195 (= N199), L214 (= L217), G216 (= G219), I217 (= I220), T218 (= T221), G253 (= G256), T254 (= T257), A255 (= A258)

1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
47% identity, 97% coverage: 9:306/306 of query aligns to 4:303/304 of 1iydA

query
sites
1iydA

F

Y

I

I

W

W

Y

F

D

N

G

G

K

E

L

M

V

V

P

R

W

W

R

E

D

D

A

A

T

K

T

V

H

H

V

V

L

M

T

S

H

H

S

A

L

L

H

H

Y

Y

G

G

L

T

S

S

V

V

F
|
F

E

E

G
|
G

V

I

R

R

A

C

Y

Y

K

D

T

S

A

H

K

K

G

G

T

P

A

V

I

V

F

F

R

R

L

H

A

R

E

E

H

H

T

M

Q

Q

R
|
R

L

L

L

H

N

D

S

S

G

A

K

K

I

I

Y

Y

M

R

M

F

D

P

I

V

P

S

Y

Q

S

S

K

I

E

D

V

E

L

L

M

M

E

E

A

A

Q

C

K

R

E

D

V

V

V

I

R

R

A

K

N

N

K

N

L

L

E

T

S

S

C

A

Y
|
Y

L

I

R

R

P

P

I

L

A

I

F

F

Y

V

G

G

S

D

E

V

K

G

M

M

G

G

I

V

S

N

T

P

R

P

-

A

G

G

A

Y

T

S

V

T

H

D

V

V

A

I

I

I

A

A

W

F

P

P

W

W

G

G

A

A

Y
|
Y

L

L

G

G

E

A

D

E

G

A

L

L

E

E

K

Q

G

G

I

I

R

D

V

A

K

M

T

V

S

S

F

W

A

N

R

R

Q

A

H

A

V

P

N

N

V

T

T

I

M

P

A

T

R

A

A

A

K
|
K

L
x
A

A

G

S

G

T

N

Y
|
Y

A

L

N

S

S

S

I

L

L

L

A

V

N

G

L

S

E

E

A

A

T

R

Q

R

D

H

G

G

Y

Y

D

Q

E

E

A

G

L

I

L

A

L

L

D

D

T

V

Q

N

G

G

F

Y

V

I

A

S

E
|
E

G

G

S

A

G
|
G

E
|
E

N

N

L

L

F

F

V

E

I

V

K

K

D

D

G

G

V

V

I

L

Y

F

E

T

P

P

E

P

I

F

-

T

A

S

S

S

A

A

L
|
L

T

P

G
|
G

I
|
I

T
|
T

R

R

D

D

S

A

V

I

I

I

R

K

V

L

A

A

R

K

E

E

L

L

G

G

Y

I

E

E

V

V

Q

R

S

E

R

Q

R

V

M

L

T

S

R

R

D

E

D

S

I

L

Y

Y

I

L

A

A

D

D

E

E

A

V

F

F

F

M

T

S

G

G

T
|
T

A
|
A

A

A

E

E

V

I

T

T

P

P

I

V

R

R

E

S

L

V

D

D

N

G

R

I

T

Q

I

V

G

G

E

E

G

G

R

R

R

C

G

G

P

P

I

V

T

T

T

K

K

R

V

I

Q

Q

A

Q

R

A

F

F

D

G

I

L

V

F

N

T

G

G

R

E

A

T

P

E

E

D

Y

K

E

W

N

G

W

W

L

L

S

D

L

Q

V

V

active site: F33 (= F38), G35 (= G40), K156 (= K160), A157 (≠ L161), E190 (= E194), L214 (= L217)
binding glutaric acid: Y92 (= Y97), Y126 (= Y130), A255 (= A258)
binding pyridoxal-5'-phosphate: R56 (= R61), K156 (= K160), Y161 (= Y165), E190 (= E194), G193 (= G197), E194 (= E198), L214 (= L217), G216 (= G219), I217 (= I220), T218 (= T221), T254 (= T257)

1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
47% identity, 97% coverage: 9:306/306 of query aligns to 4:303/304 of 1i1mA

query
sites
1i1mA

F

Y

I

I

W

W

Y

F

D

N

G

G

K

E

L

M

V

V

P

R

W

W

R

E

D

D

A

A

T

K

T

V

H

H

V

V

L

M

T

S

H

H

S

A

L

L

H

H

Y

Y

G

G

L

T

S

S

V

V

F

F

E

E

G

G

V

I

R

R

A

C

Y

Y

K

D

T

S

A

H

K

K

G

G

T

P

A

V

I

V

F

F

R

R

L

H

A

R

E

E

H

H

T

M

Q

Q

R
|
R

L

L

L

H

N

D

S

S

G

A

K

K

I

I

Y

Y

M

R

M

F

D

P

I

V

P

S

Y

Q

S

S

K

I

E

D

V

E

L

L

M

M

E

E

A

A

Q

C

K

R

E

D

V

V

V

I

R

R

A

K

N

N

K

N

L

L

E

T

S

S

C

A

Y
|
Y

L

I

R

R

P

P

I

L

A

I

F

F

Y

V

G

G

S

D

E

V

K

G

M

M

G

G

I

V

S

N

T

P

R

P

-

A

G

G

A

Y

T

S

V

T

H

D

V

V

A

I

I

I

A

A

W

F

P

P

W

W

G

G

A

A

Y

Y

L

L

G

G

E

A

D

E

G

A

L

L

E

E

K

Q

G

G

I

I

R

D

V

A

K

M

T

V

S

S

F

W

A

N

R

R

Q

A

H

A

V

P

N

N

V

T

T

I

M

P

A

T

R

A

A

A

K
|
K

L

A

A

G

S

G

T

N

Y
|
Y

A

L

N

S

S

S

I

L

L

L

A

V

N

G

L

S

E

E

A

A

T

R

Q

R

D

H

G

G

Y

Y

D

Q

E

E

A

G

L

I

L

A

L

L

D

D

T

V

Q

N

G

G

F

Y

V

I

A

S

E
|
E

G

G

S

A

G
|
G

E
|
E

N

N

L

L

F

F

V

E

I

V

K

K

D

D

G

G

V

V

I

L

Y

F

E

T

P

P

E

P

I

F

-

T

A

S

S

S

A

A

L
|
L

T

P

G
|
G

I
|
I

T
|
T

R

R

D

D

S

A

V

I

I

I

R

K

V

L

A

A

R

K

E

E

L

L

G

G

Y

I

E

E

V

V

Q

R

S

E

R

Q

R

V

M

L

T

S

R

R

D

E

D

S

I

L

Y

Y

I

L

A

A

D

D

E

E

A

V

F

F

F

M

T

S

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

T

T

P

P

I

V

R

R

E

S

L

V

D

D

N

G

R

I

T

Q

I

V

G

G

E

E

G

G

R

R

R

C

G

G

P

P

I

V

T

T

T

K

K

R

V

I

Q

Q

A

Q

R

A

F

F

D

G

I

L

V

F

N

T

G

G

R

E

A

T

P

E

E

D

Y

K

E

W

N

G

W

W

L

L

S

D

L

Q

V

V

active site: K156 (= K160)
binding 4-methyl valeric acid: Y92 (= Y97), K156 (= K160), T254 (= T257), A255 (= A258)
binding pyridoxal-5'-phosphate: R56 (= R61), K156 (= K160), Y161 (= Y165), E190 (= E194), G193 (= G197), E194 (= E198), L214 (= L217), G216 (= G219), I217 (= I220), T218 (= T221), G253 (= G256), T254 (= T257)

1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
47% identity, 97% coverage: 9:306/306 of query aligns to 4:303/304 of 1i1lA

query
sites
1i1lA

F

Y

I

I

W

W

Y

F

D

N

G

G

K

E

L

M

V

V

P

R

W

W

R

E

D

D

A

A

T

K

T

V

H

H

V

V

L

M

T

S

H

H

S

A

L

L

H

H

Y

Y

G

G

L

T

S

S

V

V

F

F

E

E

G

G

V

I

R

R

A

C

Y

Y

K

D

T

S

A

H

K

K

G

G

T

P

A

V

I

V

F

F

R

R

L

H

A

R

E

E

H

H

T

M

Q

Q

R
|
R

L

L

L

H

N

D

S

S

G

A

K

K

I

I

Y

Y

M

R

M

F

D

P

I

V

P

S

Y

Q

S

S

K

I

E

D

V

E

L

L

M

M

E

E

A

A

Q

C

K

R

E

D

V

V

V

I

R

R

A

K

N

N

K

N

L

L

E

T

S

S

C

A

Y
|
Y

L

I

R

R

P

P

I

L

A

I

F

F

Y

V

G

G

S

D

E

V

K

G

M

M

G

G

I

V

S

N

T

P

R

P

-

A

G

G

A

Y

T

S

V

T

H

D

V

V

A

I

I

I

A

A

W

F

P

P

W

W

G

G

A

A

Y

Y

L

L

G

G

E

A

D

E

G

A

L

L

E

E

K

Q

G

G

I

I

R

D

V

A

K

M

T

V

S

S

F

W

A

N

R

R

Q

A

H

A

V

P

N

N

V

T

T

I

M

P

A

T

R

A

A

A

K
|
K

L

A

A

G

S

G

T

N

Y
|
Y

A

L

N

S

S

S

I

L

L

L

A

V

N

G

L

S

E

E

A

A

T

R

Q

R

D

H

G

G

Y

Y

D

Q

E

E

A

G

L

I

L

A

L

L

D

D

T

V

Q

N

G

G

F

Y

V

I

A

S

E
|
E

G

G

S

A

G
|
G

E

E

N

N

L

L

F

F

V

E

I

V

K

K

D

D

G

G

V

V

I

L

Y

F

E

T

P

P

E

P

I

F

-

T

A

S

S

S

A

A

L

L

T

P

G
|
G

I
|
I

T
|
T

R

R

D

D

S

A

V

I

I

I

R

K

V

L

A

A

R

K

E

E

L

L

G

G

Y

I

E

E

V

V

Q

R

S

E

R

Q

R

V

M

L

T

S

R

R

D

E

D

S

I

L

Y

Y

I

L

A

A

D

D

E

E

A

V

F

F

F

M

T

S

G

G

T
|
T

A
|
A

A

A

E

E

V

I

T

T

P

P

I

V

R

R

E

S

L

V

D

D

N

G

R

I

T

Q

I

V

G

G

E

E

G

G

R

R

R

C

G

G

P

P

I

V

T

T

T

K

K

R

V

I

Q

Q

A

Q

R

A

F

F

D

G

I

L

V

F

N

T

G

G

R

E

A

T

P

E

E

D

Y

K

E

W

N

G

W

W

L

L

S

D

L

Q

V

V

active site: K156 (= K160)
binding 2-methylleucine: Y92 (= Y97), K156 (= K160), T254 (= T257), A255 (= A258)
binding pyridoxal-5'-phosphate: R56 (= R61), K156 (= K160), Y161 (= Y165), E190 (= E194), G193 (= G197), G216 (= G219), I217 (= I220), T218 (= T221), T254 (= T257)

6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
44% identity, 97% coverage: 10:306/306 of query aligns to 9:300/301 of 6thqB

query
sites
6thqB

I

V

W

W

Y

L

D

D

G

G

K

R

L

L

V

V

P

D

W

E

R

E

D

E

A

A

T

K

T

V

H

T

V

V

L

L

T

S

H

P

S

S

L

L

H

N

Y

Y

G

G

L

F

S

G

V

V

F
|
F

E

E

G

G

V

I

R

R

A

A

Y

Y

K

W

T

N

A

G

K

E

G

N

T

L

A

Y

I

V

F

F

R

R

L

L

A

R

E

D

H

H

T

M

Q

E

R
|
R

L

L

N

R

S

S

G

A

K

K

I

I

Y

I

M

G

M

L

D

D

I

V

P

P

Y

Y

S

T

K

A

E

E

V

E

L

L

M

S

E

K

A

A

Q

V

K

V

E

E

V

T

V

V

R

R

A

A

N

N

K

G

L

F

-

K

E

E

S

D

C

L

Y
|
Y

L

I

R

R

P

P

I

V

A

A

F

Y

Y

I

G

S

S

K

E

P

K

Q

M

I

G

S

I

L

S

D

T

V

R

R

G

G

A

L

T

Q

V

A

H

S

V

V

A

A

I

I

A

A

W

I

P

P

W

F

G

G

A

K

Y

Y

L

L

G

K

E

V

D

E

G

-

L

-

E

-

K

-

G

G

I

V

R

R

V

A

K

A

T

V

S

V

S

S

F

W

A

R

R

R

Q

V

H

H

V

T

N

S

V

M

T

M

M

P

A

V

R

M

A

A

K
|
K

L

A

A

T

S

G

T

I

Y
|
Y

A

L

N

N

S

S

I

I

L

M

A

A

N

A

L

V

E

E

A

A

T

R

Q

A

D

R

G

G

Y

Y

D

D

E

E

A

A

L

I

L

M

L

L

D

N

T

A

Q

E

G

G

F

K

V

V

A

V

E
|
E

G

G

S

S

G
|
G

E
|
E

N
|
N

L

I

F

F

V

I

I

V

K

R

D

R

G

G

V

V

I

L

Y

M

E

T

P

P

E

P

I

L

A

E

S

D

A

G

-

I

L
|
L

T

E

G
|
G

I
|
I

T
|
T

R

R

D

E

S

T

V

V

I

I

R

S

V

I

A

A

R

G

E

D

L

L

G

G

Y

I

E

P

V

L

Q

L

S

E

R

K

R

S

M

I

T

T

R

R

D

E

I

L

Y

Y

I

A

A

A

D

D

E

E

A

A

F

F

T

V

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

T

T

P

P

I

I

R

I

E

E

L

I

D

D

N

G

R

R

T

V

I

L

G

-

E

-

G

-

R

Q

R

R

G

G

P

P

I

I

T

T

T

Q

K

K

V

I

Q

A

A

E

R

T

F

Y

F

R

D

R

I

I

V

V

N

L

G

G

R

K

A

E

P

E

E

K

Y

Y

E

L

N

P

W

W

L

L

S

T

L

P

V

V

active site: F37 (= F38), K156 (= K160), E190 (= E194), L214 (= L217)
binding pyridoxal-5'-phosphate: R60 (= R61), K156 (= K160), Y161 (= Y165), E190 (= E194), N195 (= N199), L214 (= L217), G216 (= G219), I217 (= I220), T218 (= T221), T254 (= T257)
binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: R60 (= R61), Y97 (= Y97), K156 (= K160), Y161 (= Y165), E190 (= E194), G193 (= G197), E194 (= E198), N195 (= N199), G216 (= G219), I217 (= I220), T218 (= T221), G253 (= G256), T254 (= T257), A255 (= A258)

5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
40% identity, 92% coverage: 9:288/306 of query aligns to 3:277/290 of 5mr0D

query
sites
5mr0D

F

Y

I

V

W

Y

Y

M

D

D

G

G

K

E

L

F

V

V

P

P

W

E

R

N

D

E

A

A

T

K

T

V

H

S

V

I

L

F

T

D

H

H

S

G

L

F

H

L

Y

Y

G

G

L

D

S

G

V

V

F
|
F

E

E

G
|
G

V

I

R

R

A

A

Y

Y

K

N

T

G

A

R

K

-

G

-

T

-

A

-

I

V

F

F

R

R

L

L

A

K

E

E

H

H

T

I

Q

D

R
|
R

L

L

L

Y

N

D

S

S

G

A

K

K

I

A

Y

I

M

D

M

L

D

E

I

I

P

P

Y

I

S

T

K

K

E

E

V

E

L

F

M

M

E

E

A

I

Q

I

K

L

E

E

V

T

V

L

R

R

A

K

N

N

K

N

L

L

E

R

S

D

C

A

Y

Y

L

I

R

R

P

P

I

I

A

V

F

T

Y

R

G

G

S

I

E

G

K

D

M

L

G
|
G

I
x
L

S

D

T

P

R

R

G

K

A

C

-

Q

T

N

V

P

H

S

V

I

A

I

I

V

A

I

A

T

W

K

P

P

W

W

G

G

A

K

Y

L

L

Y

G

G

E

-

D

D

G

L

L

Y

E

E

K

K

G

G

I

L

R

T

V

A

K

I

T

T

S

V

S

A

F

V

A

R

R

R

Q

N

H

S

V

F

N

D

V

A

T

L

M

P

A

P

R

N

A

I

K
|
K

L

-

A

S

S

L

T

N

Y
|
Y

A

L

N

N

S

N

I

I

L

L

A

A

N

K

L

I

E

E

A

A

T

N

Q

A

D

K

G

G

Y

G

D

D

E

E

A

A

L

I

L

F

L

L

D

D

T

R

Q

N

G

G

F

Y

V

V

A

S

E
|
E

G

G

S

S

G
|
G

E
x
D

N

N

L

I

F

F

V

V

I

V

K

K

D

N

G

G

V

A

I

I

Y

T

E

T

P

P

E

P

I

T

A

I

S

N

A

N

L
|
L

T

R

G

G

I
|
I

T
|
T

R

R

D

E

S

A

V

V

I

I

R

E

V

I

A

I

R

N

E

R

L

L

G

G

Y

I

E

P

V

F

Q

K

S

E

R

T

R

N

M

I

T

G

R

L

D

Y

D

D

I

L

Y

Y

I

T

A

A

D

D

E

E

A

V

F

F

F

V

T

T

G
|
G

T
|
T

A

A

E

E

V

I

T

A

P

P

I

I

R

V

E

V

L

I

D

D

N

G

R

R

T

K

I

I

G

G

E

D

G

G

R

K

R

P

G

G

P

E

I

I

T

T

T

R

K

K

V

L

Q

M

A

E

R

E

F

F

active site: F32 (= F38), G34 (= G40), K150 (= K160), E183 (= E194), L206 (= L217)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (= R61), G100 (= G110), L101 (≠ I111), K150 (= K160), Y154 (= Y165), E183 (= E194), G186 (= G197), D187 (≠ E198), L206 (= L217), I209 (= I220), T210 (= T221), G245 (= G256), T246 (= T257)

5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
38% identity, 92% coverage: 9:288/306 of query aligns to 4:278/290 of 5e25A

query
sites
5e25A

F

L

I

V

W

Y

Y

M

D

N

G

G

K

E

L

F

V

V

P

P

W

E

R

S

D

Q

A

A

T

K

T

V

H

S

V

V

L

F

T

D

H

H

S

G

L

F

H

L

Y

Y

G

G

L

D

S

G

V

V

F
|
F

E

E

G
|
G

V

I

R

R

A

A

Y

Y

K

N

T

G

A

K

K

-

G

-

T

-

A

-

I

V

F

F

R

K

L

L

A

Y

E

E

H

H

T

I

Q

D

R
|
R

L

L

L

Y

N

D

S

C

G

A

K

R

I

V

Y

I

M

D

M

L

D

K

I

I

P

P

Y

L

S

S

K

K

E

E

V

E

L

F

M

A

E

E

A

A

Q

I

K

L

E

E

V

T

V

L

R

R

A

R

N

N

K

N

L

L

E

R

S

D

C

A

Y
|
Y

L

I

R

R

P

P

I

I

A

V

F

T

Y

R

G

G

S

A

E

G

K

D

M

L

G

G

I

L

S

D

T

P

R

R

G

K

A

C

-

P

T

S

V

P

H

N

V

V

A

I

I

I

A

I

A

T

W

K

P

P

W

W

G

G

A

K

Y

L

L

Y

G

G

E

-

D

D

G

L

L

Y

E

E

K

K

G

G

I

L

R

K

V

A

K

I

T

T

S

V

S

A

F

I

A

R

R

R

Q

N

H

A

V

I

N

D

V

S

T

L

M

P

A

P

R

N

A

I

K
|
K

L

-

A

S

S

L

T

N

Y
|
Y

A

L

N

N

S

N

I

I

L

L

A

A

N

K

L

I

E

E

A

A

T

N

Q

A

D

K

G

G

Y

G

D

D

E

E

A

A

L

I

L

F

L

L

D

D

T

H

Q

N

G

G

F

Y

V

I

A

S

E
|
E

G

G

S

S

G
|
G

E
x
D

N

N

L

I

F

F

V

I

I

V

K

K

D

N

G

G

V

T

I

I

Y

T

E

T

P

P

E

P

I

T

A

L

S

N

A

N

L
|
L

T

K

G
|
G

I
|
I

T
|
T

R

R

D

Q

S

V

V

V

I

I

R

E

V

L

A

I

R

N

E

E

L

L

G

E

Y

I

E

P

V

F

Q

R

S

E

R

A

R

N

M

I

T

G

R

L

D

F

D

D

I

L

Y

Y

I

S

A

A

D

D

E

E

A

I

F

F

F

V

T

T

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

T

A

P

P

I

V

R

T

E

Y

L

I

D

D

N

G

R

R

T

T

I

V

G

G

E

N

G

G

R

K

R

P

G

G

P

K

I

V

T

T

T

K

K

M

V

L

Q

M

A

E

R

K

F

F

active site: F33 (= F38), G35 (= G40), K151 (= K160), E184 (= E194), L207 (= L217)
binding 2-oxoglutaric acid: Y88 (= Y97), K151 (= K160), T247 (= T257), A248 (= A258)
binding pyridoxal-5'-phosphate: R52 (= R61), K151 (= K160), Y155 (= Y165), E184 (= E194), G187 (= G197), D188 (≠ E198), L207 (= L217), G209 (= G219), I210 (= I220), T211 (= T221), G246 (= G256), T247 (= T257)

6q8eA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum in pmp-form (see paper)
37% identity, 97% coverage: 9:306/306 of query aligns to 5:304/307 of 6q8eA

query
sites
6q8eA

F

Y

I

L

W

W

Y

W

D

N

G

H

K

R

L

I

V

I

P

P

W

W

R

E

D

E

A

A

T

T

T

V

H

H

V

L

L

T

T

D

H

Y

S

W

L

W

H

A

Y

S

G

V

L

T

S

A

V

V

F
|
F

E

E

G

G

V

I

R

R

A

G

Y

Y

-

W

K

N

T

N

A

A

K

E

G

G

T

E

A

M

-

Y

I

I

F

F

R

R

L

L

A

E

E

D

H

H

T

A

Q

R

R
|
R

L

L

L

E

N

Q

S

S

G

M

K

Q

I

L

Y

I

M

R

M

M

D

P

I

K

P

E

Y

F

S

T

K

V

E

D

V

E

L

I

M

C

E

Q

A

A

Q

T

K

I

E

D

V

L

V

V

R

R

A

A

N

N

K

D

L

Y

E

R

-

G

S

D

C

V

Y

Y

L

I

R

M

P

P

I

L

A

A

F

Y

Y

A

G

V

S

G

E

N

K

K

M

A

G

F

I

S

S

V

T

V

R

G

G

D

A

R

T

T

V

T

H

E

V

M

A

F

I

I

A

Y

A

S

W

R

P

P

W

A

G

V

A

S

Y

R

L

L

G

E

E

E

D

D

G

F

L

-

E

-

K

-

G

S

I

L

R

H

V

A

K

C

T

Y

S

S

F

W

A

T

R

R

Q

I

H

N

V

E

N

R

V

V

T

L

M

P

A

P

R

R

A

I

K
|
K

L

A

A

L

S

A

T

N

Y
|
Y

A

R

N

N

S

S

I

Q

L

L

A

A

N

S

L

S

E

E

A

A

T

A

Q

M

D

N

G

G

Y

Y

D

D

E

T

A

A

L

L

L

F

L

L

D

N

T

P

Q

E

G

G

F

K

V

V

A

A

E
|
E

G

G

S

T

G
|
G

E
x
S

N
x
C

L

V

F

F

V

F

I

V

K

R

D

K

G

G

V

K

I

L

Y

I

E

T

P

P

E

D

I

I

A

T

S

S

A

G

-

I

L
|
L

T

E

G
x
S

I
|
I

T
|
T

R

R

D

D

S

T

V

V

I

I

R

H

V

L

A

A

R

R

E

E

-

V

L

L

G

G

Y

L

E

E

V

V

Q

E

S

E

R

R

R

V

M

V

T

D

R

R

D

T

D

E

I

T

Y

Y

I

L

A

A

D

D

E

E

A

A

F

F

F

L

T

C

G
|
G

T
|
T

A

H

A

A

E

E

V

I

T

T

P

P

I

I

R

A

E

S

L

I

D

D

N

R

R

H

T

E

I

M

G

K

E

H

G

G

R

A

R

P

G

G

P

P

I

I

T

T

T

R

K

Q

V

L

Q

R

A

D

R

I

F

Y

F

R

D

E

I

V

V

V

N

Y

G

G

R

R

A

D

P

F

E

R

Y

Y

E

R

N

N

W

W

L

L

S

T

L

P

V

V

active site: F34 (= F38), K156 (= K160), E190 (= E194), L214 (= L217)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R59 (= R61), K156 (= K160), Y161 (= Y165), E190 (= E194), G193 (= G197), S194 (≠ E198), C195 (≠ N199), L214 (= L217), S216 (≠ G219), I217 (= I220), T218 (= T221), G254 (= G256), T255 (= T257)

7neaA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum (m3 mutant). (see paper)
36% identity, 97% coverage: 9:306/306 of query aligns to 5:304/309 of 7neaA

query
sites
7neaA

F

Y

I

L

W

W

Y

W

D

N

G

H

K

R

L

I

V

I

P

P

W

W

R

E

D

E

A

A

T

T

T

V

H

H

V

L

L

T

T

D

H

Y

S

W

L

W

H

A

Y

S

G

V

L

T

S

A

V

V

F
|
F

E

E

G

V

V

I

R

S

A

G

Y

Y

-

W

K

N

T

N

A

A

K

E

G

G

T

E

A

M

-

Y

I

I

F

F

R

R

L

L

A

E

E

D

H

H

T

A

Q

R

R
|
R

L

L

L

E

N

Q

S

S

G

M

K

Q

I

L

Y

I

M

R

M

M

D

P

I

K

P

E

Y

F

S

T

K

V

E

D

V

E

L

I

M

C

E

Q

A

A

Q

T

K

I

E

D

V

L

V

V

R

R

A

A