SitesBLAST

P07342 Acetolactate synthase catalytic subunit, mitochondrial; Acetohydroxy-acid synthase catalytic subunit; AHAS; ALS; EC 2.2.1.6 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
44% identity, 99% coverage: 3:564/570 of query aligns to 92:665/687 of P07342

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P07342

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R241 (= R152) binding FAD
355:376 (vs. 258:279, 50% identical) binding FAD
407:426 (vs. 302:321, 30% identical) binding FAD

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
43% identity, 99% coverage: 5:568/570 of query aligns to 14:580/582 of 8et4A

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8et4A

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binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V393), G401 (= G394), Q402 (= Q395), H403 (= H396), G426 (= G419), M428 (= M421), G452 (= G445), D453 (≠ E446), G454 (≠ A447), S455 (= S448), M458 (= M451), N480 (= N473), H482 (≠ Y475), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ P255), M266 (= M257), H267 (= H258), G286 (= G277), V287 (≠ A278), R288 (= R279), D290 (= D281), R292 (= R283), V293 (= V284), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ L322), M405 (= M398), G423 (= G416)
binding magnesium ion: F370 (≠ Y363), D453 (≠ E446), M458 (= M451), Q461 (= Q454), N480 (= N473), H482 (≠ Y475), K533 (≠ P519)
binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (= M257), R292 (= R283), M485 (= M478), W489 (= W482), S568 (≠ G556)

5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
43% identity, 99% coverage: 5:568/570 of query aligns to 14:580/582 of 5wj1A

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5wj1A

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N

F

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F

A

A

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A

A

A

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C

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G

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H

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x
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active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ V29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M257), V293 (= V284), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ P546)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), M263 (= M254), L264 (≠ P255), G286 (= G277), R288 (= R279), V293 (= V284), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ L322), M405 (= M398), G423 (= G416), G424 (= G417)
binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ Y475)
binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (= M257), D291 (= D282), R292 (= R283), M485 (= M478), W489 (= W482), S568 (≠ G556)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V393), G401 (= G394), Q402 (= Q395), H403 (= H396), M428 (= M421), D453 (≠ E446), G454 (≠ A447), S455 (= S448), M458 (= M451), N480 (= N473), H482 (≠ Y475), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)

5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium (see paper)
43% identity, 99% coverage: 5:568/570 of query aligns to 14:580/582 of 5k6tA

query
sites
5k6tA

G

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F

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A

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C

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G

N

P

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Y

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H

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H

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G
x
S

G

G

K

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active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ V29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M257), V293 (= V284), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ P546)
binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: H267 (= H258), R292 (= R283), M485 (= M478), W489 (= W482), S568 (≠ G556)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ P255), G286 (= G277), R288 (= R279), D290 (= D281), R292 (= R283), V293 (= V284), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ L322), Q404 (= Q397), M405 (= M398), G423 (= G416)
binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ Y475)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V393), G401 (= G394), Q402 (= Q395), H403 (= H396), G426 (= G419), M428 (= M421), G452 (= G445), G454 (≠ A447), S455 (= S448), N480 (= N473), H482 (≠ Y475), L483 (= L476), G484 (= G477)

5k6rA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, thiencarbazone-methyl (see paper)
43% identity, 99% coverage: 5:568/570 of query aligns to 14:580/582 of 5k6rA

query
sites
5k6rA

G

G

A

A

E

D

I

I

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I

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C

A

L

L

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E

E

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Y

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N

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L

L

F

A

A

S

A

A

A

Y

C

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G

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A

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A

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R

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N

P

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Y

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x
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H

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x
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active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ V29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M257), V293 (= V284), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ P546)
binding methyl 4-[(3-methoxy-4-methyl-5-oxidanylidene-1,2,4-triazol-1-yl)carbonylsulfamoyl]-5-methyl-thiophene-3-carboxylate: R292 (= R283), W489 (= W482), S568 (≠ G556)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ P255), M266 (= M257), G286 (= G277), R288 (= R279), R292 (= R283), V293 (= V284), D310 (= D302), I311 (= I303), G328 (= G320), D329 (= D321), V330 (≠ L322), M405 (= M398), G423 (= G416)
binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ Y475)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V393), G401 (= G394), Q402 (= Q395), H403 (= H396), G426 (= G419), M428 (= M421), D453 (≠ E446), G454 (≠ A447), S455 (= S448), M458 (= M451), N480 (= N473), H482 (≠ Y475), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)

1z8nA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with an imidazolinone herbicide, imazaquin (see paper)
43% identity, 99% coverage: 5:568/570 of query aligns to 14:580/582 of 1z8nA

query
sites
1z8nA

G

G

A

A

E

D

I

I

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I

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A

L

L

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Y

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A
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E

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C

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N

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K

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x
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L

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W

Q

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L

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F

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L

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D

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P

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N

F

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A

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K

L

L

F

A

A

S

A

A

A

Y

C

G

G

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A

I

A

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K

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A

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V

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G

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A

A

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T

A

M

F

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D

T

T

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P

K

G

N

P

D

Y

L

L

V

-

F

-

L

L

D

D

F

V

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D

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P
x
H

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E

N

H

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V

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L

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M

M

V

I

Q

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G

S

G
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G

K

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I

I

L

T

S

E

A

G

E

D

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ V29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M257), V293 (= V284), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ P546)
binding 2-(4-isopropyl-4-methyl-5-oxo-4,5-dihydro-1h-imidazol-2-yl)quinoline-3-carboxylic acid: K135 (= K126), R161 (= R152), Y191 (= Y182), R194 (vs. gap), D291 (= D282), R292 (= R283), D312 (= D304), W489 (= W482), G569 (= G557)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G211), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ P255), G265 (= G256), M266 (= M257), H267 (= H258), G286 (= G277), V287 (≠ A278), R288 (= R279), D290 (= D281), R292 (= R283), V293 (= V284), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ L322), M405 (= M398), G423 (= G416), G424 (= G417)
binding magnesium ion: D453 (≠ E446), N480 (= N473)
binding thiamine diphosphate: V400 (= V393), G401 (= G394), Q402 (= Q395), H403 (= H396), G426 (= G419), M428 (= M421), G452 (= G445), G454 (≠ A447), S455 (= S448), N480 (= N473), H482 (≠ Y475), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)

1yi1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
43% identity, 99% coverage: 5:568/570 of query aligns to 14:580/582 of 1yi1A

query
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active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ V29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M257), V293 (= V284), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ P546)
binding methyl 2-[4-methoxy-6-methyl-1,3,5-trazin-2-yl(methyl)carbamoylsulfamoyl]benzoate: D291 (= D282), R292 (= R283), W489 (= W482), S568 (≠ G556)
binding flavin-adenine dinucleotide: R161 (= R152), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), M263 (= M254), L264 (≠ P255), G265 (= G256), M266 (= M257), H267 (= H258), G286 (= G277), V287 (≠ A278), R288 (= R279), D290 (= D281), V293 (= V284), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ L322), M405 (= M398), G423 (= G416), G424 (= G417)
binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ Y475)

1yi0A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
43% identity, 99% coverage: 5:568/570 of query aligns to 14:580/582 of 1yi0A

query
sites
1yi0A

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active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ V29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M257), V293 (= V284), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ P546)
binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (= D282), R292 (= R283), W489 (= W482), S568 (≠ G556)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ P255), G265 (= G256), M266 (= M257), H267 (= H258), G286 (= G277), V287 (≠ A278), R288 (= R279), D290 (= D281), R292 (= R283), V293 (= V284), D310 (= D302), I311 (= I303), G328 (= G320), D329 (= D321), V330 (≠ L322), M405 (= M398), G423 (= G416), G424 (= G417)
binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ Y475)

1yhzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
43% identity, 99% coverage: 5:568/570 of query aligns to 14:580/582 of 1yhzA

query
sites
1yhzA

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active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ V29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M257), V293 (= V284), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ P546)
binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: D291 (= D282), R292 (= R283), M485 (= M478), W489 (= W482), S568 (≠ G556)
binding flavin-adenine dinucleotide: R161 (= R152), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ P255), M266 (= M257), H267 (= H258), G286 (= G277), V287 (≠ A278), R288 (= R279), D290 (= D281), V293 (= V284), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ L322), Q404 (= Q397), M405 (= M398), G423 (= G416), G424 (= G417)
binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ Y475)

1yhyA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
43% identity, 99% coverage: 5:568/570 of query aligns to 14:580/582 of 1yhyA

query
sites
1yhyA

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active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ V29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M257), V293 (= V284), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ P546)
binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (= D282), R292 (= R283), V486 (= V479), W489 (= W482), S568 (≠ G556)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ P255), G265 (= G256), M266 (= M257), H267 (= H258), G286 (= G277), V287 (≠ A278), R288 (= R279), D290 (= D281), V293 (= V284), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ L322), Q404 (= Q397), M405 (= M398), G423 (= G416), G424 (= G417)
binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ Y475)

1ybhA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide chlorimuron ethyl (see paper)
43% identity, 99% coverage: 5:568/570 of query aligns to 14:580/582 of 1ybhA

query
sites
1ybhA

G

G

A

A

E

D

I

I

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L

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V

R

E

C

A

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L

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E

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Y
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Y

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P

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A
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A

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x
S

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M

Y

E

I

I

Y

H

D

Q

A

A

L

L

F

T

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R

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N

V

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R

R

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E
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E

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Q

A

G

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V

H

F

A

A

D

E

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F

Y

A

A

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E

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K

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T

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S

G

G

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P

G

G

A

A

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T

N

N

A

L

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G

G

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A

A

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A

A

F

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C

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M

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A

I

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Q

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M

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I

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G

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T

D

D

A

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F

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E
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E

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T

D

P

T

I

V

V

G

E

I

V

T

T

R

R

P

S

C

I

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T

K

K

H

H

N

N

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Y

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M

D

D

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V

R

E

D

D

I

I

A

P

A

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T

I

I

I

K

E

A

A

F

F

Y

F

L

L

A

A

K

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G

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R
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R

P

P

G

G

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P

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L

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D

D

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K

D

D

I

I

T

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I

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A

Q

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C

A

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F

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Y

W

P

E

K

Q

E

A

I

M

S

R

M

L

R

P

S

G

Y

Y

-

M

-

S

-

R

-

M

-

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S

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P

P

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P

V

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K

D

G

S

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H

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L

G

E

Q

Q

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I

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-

A

-

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V

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L

L

L

I

L

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E

E

A

S

K

K

R

K

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P

M

V

I

L

Y

Y

T

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G

G

G
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G

V

C

V

L

L

-

A

-

N

N

A

S

E

D

Q

E

L

L

T

G

K

R

L

F

T

V

R

E

L

L

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P

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T

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M
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M

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L

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Y

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M

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x
L

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M

H
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H

G

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T

T

Y

V

E

Y

A

A

N

N

M

Y

A

A

M

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G

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x
V

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R

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R
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V

I

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G

G

N

K

P

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A

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-

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A

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K

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I
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I

D

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P

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V

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-

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N

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L

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L

L

-

D

-

F

A

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Y

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E

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T

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A

A

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D

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V

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G

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M
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M

W

W

A

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A

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Y

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Y

Y

R

N

F

Y

D

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W

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M

G

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L

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Y

A

A

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I

G

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A

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A

A

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N

P

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x
D

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E

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N

L

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K

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C

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L

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N

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x
H

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L

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M

V
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V

R

M

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W
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W

Q

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Q

D

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F

F

H

Y

G

K

G

A

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N

Y

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L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

D

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L

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P

P

D

N

F

M

A

L

K

L

L

F

A

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A

A

A

Y

C

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G

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I

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A

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A

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R

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V

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K

A

A

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D

V

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G

E

A

A

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T

T

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G

N

P

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Y

L

L

V

-

F

-

L

L

D

D

F

V

Q

I

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P

P
x
H

T

Q

E

E

N

H

V

V

Y

L

P

P

M

M

V

I

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G
x
S

G

G

K

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L

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E

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M

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E

A

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E

D

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ V29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M257), V293 (= V284), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ P546)
binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: M266 (= M257), D291 (= D282), R292 (= R283), M485 (= M478), W489 (= W482), S568 (≠ G556)
binding flavin-adenine dinucleotide: R161 (= R152), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ P255), M266 (= M257), H267 (= H258), G286 (= G277), V287 (≠ A278), R288 (= R279), D290 (= D281), V293 (= V284), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ L322), Q404 (= Q397), M405 (= M398), G423 (= G416), G424 (= G417)
binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ Y475)

P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
43% identity, 99% coverage: 5:568/570 of query aligns to 99:665/670 of P17597

query
sites
P17597

G

G

A

A

E

D

I

I

V

L

I

V

R

E

C

A

L

L

Q

E

E

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G

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F

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A

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Y

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A
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A

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x
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A

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N

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E

Q

Q

A

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V

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A

A

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E

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Y

A

A

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A

C

L

I

V

A

T

T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

A

L

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V

T

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G

G

I

L

A

A

T

D

A

A

F

L

C

L

D

D

S
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S

I

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P

M

L

V

V

I

A

I

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G

G

Q

Q

V

V

P
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T

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A
x
R

A

M

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I

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G

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T

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D

A

A

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F

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Q

E

E

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V

G

E

I

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R

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K

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H

N

N

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M

D

D

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V

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E

D

D

I

I

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A

A

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L

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A

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R

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K

D

D

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-

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-

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Q

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Y

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G

G

G
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G

G

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C

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L

-

A

-

N

N

A

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T

L
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L

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M

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x
C

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x
L

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G

M
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M

H
|
H

G

G

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T

Y

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Y

A

A

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N

M

Y

A

A

M

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A

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G

A
x
V

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R

F
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F

D
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D

R
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R

V

V

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G

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A

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K

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D

I
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I

D

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V

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M

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G

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H

Q

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M

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A

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Y

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Y

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M

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L

L

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N

L

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H

L
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L

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M

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W

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L

-

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A

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D

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A

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A

A

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L

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P

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G

N

P

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L

L

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-

F

-

L

L

D

D

F

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D

P

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E

N

H

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L

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M

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G
x
S

G

G

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G

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D

A122 (= A28) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
M124 (≠ L30) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
E144 (= E50) binding thiamine diphosphate
S186 (= S92) binding FAD
P197 (= P103) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
R199 (≠ A105) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
Q207 (= Q113) binding thiamine diphosphate
K220 (= K126) binding (R)-imazaquin
R246 (= R152) binding (R)-imazaquin; binding FAD
K256 (= K162) binding chlorimuron-ethyl
G308 (= G212) binding FAD
TL 331:332 (= TL 237:238) binding FAD
C340 (≠ A246) modified: Cysteine sulfinic acid (-SO2H)
LGMH 349:352 (≠ PGMH 255:258) binding FAD
GVRFD 371:375 (≠ GARFD 277:281) binding FAD
DR 376:377 (= DR 282:283) binding chlorimuron-ethyl
DI 395:396 (= DI 302:303) binding FAD
DV 414:415 (≠ DL 321:322) binding FAD
QH 487:488 (= QH 395:396) binding thiamine diphosphate
GG 508:509 (= GG 416:417) binding FAD
GAM 511:513 (≠ GTM 419:421) binding thiamine diphosphate
D538 (≠ E446) binding Mg(2+)
DGS 538:540 (≠ EAS 446:448) binding thiamine diphosphate
N565 (= N473) binding Mg(2+)
NQHLGM 565:570 (≠ NRYLGM 473:478) binding thiamine diphosphate
H567 (≠ Y475) binding Mg(2+)
W574 (= W482) binding chlorimuron-ethyl; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.
S653 (≠ G556) binding chlorimuron-ethyl; mutation to A: No effect on catalytic activity or sensitivity to herbicides.; mutation to F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; mutation to N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; mutation to T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.

5k2oA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, pyrithiobac (see paper)
43% identity, 99% coverage: 5:568/570 of query aligns to 14:580/585 of 5k2oA

query
sites
5k2oA

G

G

A

A

E

D

I

I

V

L

I

V

R

E

C

A

L

L

Q

E

E

R

E

Q

K

G

V

V

E

E

Y

T

V

V

F

F

G

A

Y
|
Y

P

P

G
|
G

A
|
A

V
x
S

L

M

Y

E

I

I

Y

H

D

Q

A

A

L

L

F

T

Q

R

Q

S

E

S

Q

S

V

I

R

R

H

N

V

V

L

L

V

P

R

R

H

H

E
|
E

Q

Q

A

G

V

V

H

F

A

A

D

E

G

G

F

Y

A

A

R

R

S

S

T

S

E

G

K

K

V

P

G

G

V

I

A

C

L

I

V

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

A

L

V

V

T

S

G

G

I

L

A

A

T

D

A

A

F

L

C

L

D

D

S

S

I

V

P

P

M

L

V

V

I

A

I

I

S

T

G

G

Q

Q

V

V

P

P

T

R

A

R

A

M

I

I

G

G

Q

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

V

T

D

P

T

I

V

V

G

E

I

V

T

T

R

R

P

S

C

I

V

T

K

K

H

H

N

N

F

Y

L

L

V

V

K

M

D

D

V

V

R

E

D

D

I

I

A

P

A

R

T

I

I

I

K

E

A

A

F

F

Y

F

L

L

A

A

K

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

I

V

P

P

K
|
K

D

D

I

I

T

Q

I

Q

A

Q

K

L

C

A

D

I

F

P

E

N

Y

W

P

E

K

Q

E

A

I

M

S

R

M

L

R

P

S

G

Y

Y

-

M

-

S

-

R

-

M

-

P

S

K

P

P

V

P

V

E

K

D

G

S

H

H

Q

L

G

E

Q

Q

I

I

K

-

A

-

V

V

Q

R

L

L

L

I

L

S

E

E

A

S

K

K

R

K

P

P

M

V

I

L

Y

Y

T

V

G
|
G

V

C

V

L

L

-

A

-

N

N

A

S

E

D

Q

E

L

L

T

G

K

R

L

F

T

V

R

E

L

L

L

T

G

G

F

I

P

P

V

V

T

A

N

S

T
|
T

L
|
L

M
|
M

G

G

L

L

G

G

G

S

Y

Y

P

P

A

C

S

D

D

D

R

E

Q

L

Y

S

L

L

G

H

M

M

P
x
L

G

G

M
|
M

H

H

G

G

T

T

Y

V

E

Y

A

A

N

N

M

Y

A

A

M

V

H

E

Y

H

S

S

D

D

V

L

L

L

A

A

V

F

G
|
G

A

V

R
|
R

F

F

D
|
D

D

D

R
|
R

V
|
V

I

T

G

G

N

K

P

L

A

E

N

A

F

F

A

A

E

S

E

R

P

-

R

A

K

K

I

I

I

V

H

H

I

I

D
|
D

I
|
I

D

D

P

S

S

A

S

E

I

I

S

G

K

K

R

N

V

K

K

T

V

P

D

H

V

V

P

S

I

V

V

C

G

G

D
|
D

L
x
V

K

K

D

L

V

A

L

L

D

Q

E

G

M

M

I

N

R

K

Q

V

L

L

E

E

A

-

P

-

T

N

R

R

P

A

D

E

A

E

S

L

N

K

L

L

-

D

-

F

A

G

A

V

W

W

W

R

K

N

Q

E

V

L

E

N

E

V

W

Q

R

K

S

Q

R

K

K

F

C

P

M

L

E

S

Y

F

K

K

N

T

S

F

D

G

E

E

I

A

I

I

K

P

P

P

Q

Q

F

Y

V

A

V

I

Q

K

K

V

L

L

W

D

E

E

V

L

T

T

G

D

G

G

D

K

A

A

I

I

V

I

T

S

S

T

D

G

V
|
V

G
|
G

Q
|
Q

H
|
H

Q
|
Q

M
|
M

W

W

A

A

S

A

Q

Q

Y

F

Y

Y

R

N

F

Y

D

K

K

K

P

P

R

R

R

Q

W

W

L

L

N

S

S

S

G
|
G

G

G

L

L

G
|
G

T

A

M
|
M

G

G

V

F

G

G

L

L

P

P

Y

A

A

A

M

I

G

G

A

A

Q

S

F

V

A

A

H

N

P

P

D

D

M

A

P

I

V

V

A

V

C

D

V

I

T

D

G

G

E
x
D

A
x
G

S
|
S

I

F

Q

I

M

M

C

N

I

V

Q

Q

E

E

L

L

S

A

T

T

C

I

K

R

Q

V

F

E

R

N

L

L

P

P

I

V

K

K

I

V

C

L

N

L

L

L

N

N

N
|
N

R

Q

Y
x
H

L
|
L

G
|
G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

Q

D

L

R

F

F

H

Y

G

K

G

A

R

N

Y

R

S

A

E

H

S

T

Y

F

M

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

D

E

S

I

L

F

P

P

D

N

F

M

A

L

K

L

L

F

A

A

S

A

A

A

Y

C

G

G

H

I

V

P

G

A

I

A

R

R

V

V

D

T

K

K

P

K

A

A

D

D

V

L

E

R

G

E

A

A

L

I

R

Q

E

T

A

M

F

L

T

D

T

T

H

P

K

G

N

P

D

Y

L

L

V

-

F

-

L

L

D

D

F

V

Q

I

V

C

D

P

P
x
H

T

Q

E

E

N

H

V

V

Y

L

P

P

M

M

V

I

Q

P

G
x
S

G

G

K

G

G

T

L

F

T

N

E

D

M

V

I

I

L

T

S

E

A

G

E

D

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ V29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M257), V293 (= V284), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ P546)
binding 2-chloranyl-6-(4,6-dimethoxypyrimidin-2-yl)sulfanyl-benzoic acid: M266 (= M257), R292 (= R283), W489 (= W482), S568 (≠ G556)
binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ P255), G286 (= G277), R288 (= R279), D290 (= D281), V293 (= V284), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ L322), Q404 (= Q397), M405 (= M398), G423 (= G416)
binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ Y475)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V393), G401 (= G394), Q402 (= Q395), H403 (= H396), M428 (= M421), D453 (≠ E446), G454 (≠ A447), S455 (= S448), N480 (= N473), H482 (≠ Y475), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)

5wkcA Saccharomyces cerevisiae acetohydroxyacid synthase in complex with the herbicide penoxsulam (see paper)
44% identity, 99% coverage: 3:564/570 of query aligns to 8:569/591 of 5wkcA

query
sites
5wkcA

L

L

T

T

G

G

A

G

E

Q

I

I

V

F

I

N

R

E

C

M

L

M

Q

S

E

R

E

Q

K

N

V

V

E

D

Y

T

V

V

F

F

G

G

Y
|
Y

P
|
P

G
|
G

A
|
A

V
x
I

L

L

Y

P

I

V

Y

Y

D

D

A

A

L

I

F

H

Q

N

Q

S

E

D

Q

K

V

F

R

N

H

F

V

V

L

L

V

P

R

K

H

H

E
|
E

Q

Q

A

G

A

A

V

G

H

H

A

M

A

A

D

E

G

G

F

Y

A

A

R

R

S

A

T

S

E

G

K

K

V

P

G

G

V

V

A

V

L

L

V

V

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

A

V

V

V

T

T

G

P

I

M

A

A

T

D

A

A

F

F

C

A

D

D

S

G

I

I

P

P

M

M

V

V

I

V

I

F

S

T

G

G

Q

Q

V
|
V

P

P

T

T

A

S

A

A

I

I

G

G

Q

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

V

A

D

D

T

V

V

V

G

G

I

I

T

S

R

R

P

S

C

C

V

T

K

K

H

W

N

N

F

V

L

M

V

V

K

K

D

S

V

V

R

E

D

E

I

L

A

P

A

L

T

R

I

I

K

N

K

E

A

A

F

F

Y

E

L

I

A

A

K

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

V

V

D

D

I

L

P

P

K
|
K

D

D

I

V

T

T

I

A

A

A

K

I

C

L

D

R

F

N

E

P

Y

I

P

P

K

T

E

K

I

T

S

T

M

A

R

Q

S

D

Y

E

S

F

P

V

V

M

V

-

K

-

G

-

H

-

Q

-

G

Q

Q

S

I

I

K

N

K

K

A

A

V

A

Q

D

L

L

L

I

L

N

E

L

A

A

K

K

R

K

P

P

M

V

I

L

Y

Y

T

V

G
|
G

G
x
A

G
|
G

V

I

V

L

-
x
N

L

H

A

A

N

D

A

G

A

P

E
x
R

Q

L

L

L

T

K

K

E

L

L

T

S

R

D

L

R

L

A

G

Q

F

I

P

P

V

V

T

T

N

T

T
|
T

L
|
L

M
x
Q

G

G

L

L

G

G

G

S

Y

F

P

D

A

Q

S

E

D

D

R

P

Q

K

Y

S

L

L

G

D

M

M

P
x
L

G

G

M
|
M

H

H

G

G

T

C

Y

A

E

T

A

A

N

N

M

L

A

A

M

V

H

Q

Y

N

S

A

D

D

V

L

L

I

A

A

V

V

G
|
G

A
|
A

R
|
R

F

F

D

D

R
|
R

V
|
V

I

T

G

G

N

N

P

I

A

S

N

K

F

F

A

A

E

P

E

E

P

A

R

R

K

R

-

A

-

E

-

G

-

R

-

G

I

I

H

H

I

F

D
x
E

I
x
V

D

S

P

P

S

K

S
x
N

I

I

S

N

K

K

R

V

V

V

K

Q

V

T

D

Q

V

I

P

A

I

V

V

E

G

G

D
|
D

L
x
A

K

T

D

T

V

N

L

L

D

G

E

K

M

M

I

M

R

S

Q

K

L

I

E

F

A

P

A

V

P

K

T

E

R

R

P

S

D

E

A

-

S

-

N

-

L

-

A

-

W

W

W

F

K

A

Q

Q

V

I

E

N

E

K

W

W

R

K

S

K

R

E

K

Y

C

P

M

Y

E

A

Y

Y

-

M

-

E

K

E

N

T

S

P

D

G

E

S

I

K

I

I

K

K

P

P

Q

Q

F

T

V

V

V

I

Q

K

K

K

L

L

W

S

E

K

V

V

-

A

-

N

-

D

T

T

G

G

G

R

D

H

A

V

I

I

V

V

T

T

S

T

D

G

V
|
V

G
|
G

Q
|
Q

H
|
H

Q

Q

M
|
M

W

W

A

A

S

A

Q

Q

Y

H

Y

W

R

T

F

W

D

R

K

N

P

P

R

H

R

T

W

F

L

I

N

T

S

S

G
|
G

G

G

L
|
L

G
|
G

T

T

M
|
M

G

G

V

Y

G

G

L

L

P

P

Y

A

A

A

M

I

G

G

A

A

Q

Q

F

V

A

A

H

K

P

P

D

E

M

S

P

L

V

V

A

I

C

D

V

I

T

D

G
|
G

E
x
D

A
|
A

S
|
S

I

F

Q

N

M
|
M

C

T

I

L

Q

T

E

E

L

L

S

S

T

S

C

A

K

V

Q

Q

F

A

R

G

L

T

P

P

I

V

K

K

I

I

C

L

N

I

L

L

N

N

N
|
N

R

E

Y
x
E

L
x
Q

G
|
G

M
|
M

V
|
V

R

T

Q

Q

W
|
W

Q

Q

Q

S

L

L

F

F

H

Y

G

E

G

H

R

R

Y

Y

S

S

E

H

S

T

Y

H

M

Q

D

L

S

N

L

-

P

P

D

D

F

F

A

I

K

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G

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R

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L

R

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E

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F

F

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I

active site: Y29 (= Y24), G31 (= G26), G32 (= G27), A33 (= A28), I34 (≠ V29), E55 (= E50), T78 (= T73), F117 (= F112), Q118 (= Q113), E119 (= E114), K167 (= K162), R222 (≠ E221), M258 (= M257), V285 (= V284), V401 (= V393), L426 (= L418), G427 (= G419), M429 (= M421), D454 (≠ E446), N481 (= N473), E483 (≠ Y475), Q484 (≠ L476), M486 (= M478), V487 (= V479), W490 (= W482), L512 (= L505), G517 (= G510), L518 (≠ H511), K551 (≠ P546)
binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V401 (= V393), G402 (= G394), Q403 (= Q395), H404 (= H396), G427 (= G419), M429 (= M421), G453 (= G445), D454 (≠ E446), A455 (= A447), S456 (= S448), M459 (= M451), N481 (= N473), E483 (≠ Y475), Q484 (≠ L476), G485 (= G477), M486 (= M478), V487 (= V479)
binding ethaneperoxoic acid: G32 (= G27), Q118 (= Q113)
binding flavin-adenine dinucleotide: R157 (= R152), G211 (= G211), A212 (≠ G212), G213 (= G213), N216 (vs. gap), T238 (= T237), L239 (= L238), Q240 (≠ M239), L256 (≠ P255), M258 (= M257), G278 (= G277), A279 (= A278), R280 (= R279), R284 (= R283), V285 (= V284), E311 (≠ D302), V312 (≠ I303), N316 (≠ S307), D330 (= D321), A331 (≠ L322), M406 (= M398), G424 (= G416)
binding magnesium ion: D454 (≠ E446), N481 (= N473), E483 (≠ Y475)
binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: G32 (= G27), A33 (= A28), V107 (= V102), F117 (= F112), K167 (= K162), M258 (= M257), R284 (= R283), M486 (= M478), W490 (= W482)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: P30 (= P25), E55 (= E50)

Query Sequence

>WP_011766879.1 NCBI__GCF_000061505.1:WP_011766879.1
MVLTGAEIVIRCLQEEKVEYVFGYPGGAVLYIYDALFQQEQVRHVLVRHEQAAVHAADGF
ARSTEKVGVALVTSGPGATNAVTGIATAFCDSIPMVIISGQVPTAAIGQDAFQEVDTVGI
TRPCVKHNFLVKDVRDIAATIKKAFYLAKTGRPGPVLVDIPKDITIAKCDFEYPKEISMR
SYSPVVKGHQGQIKKAVQLLLEAKRPMIYTGGGVVLANAAEQLTKLTRLLGFPVTNTLMG
LGGYPASDRQYLGMPGMHGTYEANMAMHYSDVLLAVGARFDDRVIGNPANFAEEPRKIIH
IDIDPSSISKRVKVDVPIVGDLKDVLDEMIRQLEAAPTRPDASNLAAWWKQVEEWRSRKC
MEYKNSDEIIKPQFVVQKLWEVTGGDAIVTSDVGQHQMWASQYYRFDKPRRWLNSGGLGT
MGVGLPYAMGAQFAHPDMPVACVTGEASIQMCIQELSTCKQFRLPIKICNLNNRYLGMVR
QWQQLFHGGRYSESYMDSLPDFAKLASAYGHVGIRVDKPADVEGALREAFTTHKNDLVFL
DFQVDPTENVYPMVQGGKGLTEMILSAEDL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory