SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011767066.1 NCBI__GCF_000061505.1:WP_011767066.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

6j2lA Crystal structure of bi-functional enzyme (see paper)
46% identity, 74% coverage: 10:106/131 of query aligns to 8:104/200 of 6j2lA

query
sites
6j2lA

E

E

V

L

K

D

W

W

D

E

E

K

-

T

N

D

G

G

L

L

V

M

P

P

V

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I

A

V

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Q

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H

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A

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S

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G

D

E

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V

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L

M

M

F

L

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W

Y

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M

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L

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L

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W

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D
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D

C

C

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D

N

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D

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L

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C
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C

H

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C

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F

binding zinc ion: D79 (= D80), D81 (= D82), D83 (= D84), C96 (= C98), C103 (= C105)

Sites not aligning to the query:

binding zinc ion: 152, 171, 174

6j2lB Crystal structure of bi-functional enzyme (see paper)
44% identity, 68% coverage: 10:98/131 of query aligns to 7:96/185 of 6j2lB

query
sites
6j2lB

E

E

V

L

K

D

W

W

D

E

E

K

-

T

N

D

G

G

L

L

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S

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G

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G

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R

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K

R

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L

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W

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L

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S

L

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I

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D
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D

C

C

D
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D

N

N

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D

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V

L

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S

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C

binding zinc ion: D78 (= D80), D80 (= D82), D82 (= D84)

Sites not aligning to the query:

binding magnesium ion: 137, 140, 156, 159

7bgmA Crystal structure of mthisn2, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
39% identity, 86% coverage: 8:120/131 of query aligns to 8:119/213 of 7bgmA

query
sites
7bgmA

L

L

N

D

E

S

V

V

K

K

W

W

D

D

E

N

N

K

G

G

L

L

V

A

P

V

V

A

I

I

A

A

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Q

E

N

A

V

S

D

T

T

G

G

D

A

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L

L

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M

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A

G

W

F

M

A

N

N

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A

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G

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E

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C

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V

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G

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C
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C

H

H

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G

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C

F

Y

F

Y

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D

D

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W

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E

binding zinc ion: D80 (= D80), D82 (= D82), D84 (= D84), C97 (= C98), C104 (= C105)

Sites not aligning to the query:

binding zinc ion: 163, 183, 186

7bgnA Crystal structure of mthisn2-amp complex, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
41% identity, 76% coverage: 8:107/131 of query aligns to 6:104/204 of 7bgnA

query
sites
7bgnA

L

L

N

D

E

S

V

V

K

K

W

W

D

D

E

N

N

K

G

G

L

L

V

A

P

V

V

A

I

I

A

A

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N

A

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T

T

G

G

D

A

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L

L

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M

A

N

N

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E

A

A

L

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A

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T

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E

S

T

S

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R

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K

A

A

I

T

Y

F

W

Y

S

S

R

R

S

S

R

R

R

S

K

S

L

L

W
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W

H

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K
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K

G
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G

E

E

E
x
T

S
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S

G

N

H

N

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F

Q

I

K

N

V

V

V

H

E

D

L

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D

C

C

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D

N

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D

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G

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x
T

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C

H
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H

T

T

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G

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C

F

Y

F

Y

binding adenosine monophosphate: W60 (= W62), K62 (= K64), G63 (= G65), T65 (≠ E67), S66 (= S68), T94 (≠ A97), H96 (= H99)
binding zinc ion: D78 (= D80), D80 (= D82), D82 (= D84), C95 (= C98), C102 (= C105)

Sites not aligning to the query:

binding adenosine monophosphate: 121, 128, 135, 136, 137, 180, 203, 204
binding zinc ion: 154, 174, 177

Query Sequence

>WP_011767066.1 NCBI__GCF_000061505.1:WP_011767066.1
MSTSTKWLNEVKWDENGLVPVIAQEASTGDVLMFAWMNREALQRTAETGNAIYWSRSRRK
LWHKGEESGHVQKVVELRIDCDNDVVLMKVEQVGGIACHTGRHSCFFQKYLADGRWETVE
PVLKDPKDIYS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory