SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011767245.1 NCBI__GCF_000061505.1:WP_011767245.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WQB3 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
55% identity, 97% coverage: 5:556/572 of query aligns to 42:644/644 of P9WQB3

query
sites
P9WQB3

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51:368 (vs. 17:335, 66% identical) N-terminal domain
R80 (= R47) binding 3-methyl-2-oxobutanoate
T254 (= T221) binding 3-methyl-2-oxobutanoate
H285 (= H252) binding 3-methyl-2-oxobutanoate
H287 (= H254) binding 3-methyl-2-oxobutanoate
369:424 (vs. 336:381, 54% identical) Subdomain I
425:433 (vs. 382:390, 89% identical) Linker
426:644 (vs. 383:556, 39% identical) Required for the condensation reaction. Not required to bind substrate
434:490 (vs. 391:444, 53% identical) Subdomain II
491:644 (vs. 445:556, 31% identical) Regulatory domain
N532 (= N483) binding L-leucine
A536 (≠ D487) binding L-leucine
D563 (≠ G511) binding L-leucine
A565 (= A513) binding L-leucine
P625 (≠ A537) binding L-leucine
I627 (= I539) binding L-leucine

3figB Crystal structure of leucine-bound leua from mycobacterium tuberculosis (see paper)
56% identity, 97% coverage: 5:556/572 of query aligns to 25:577/577 of 3figB

query
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3figB

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V

I

I

R

R

V

V

N

N

S

-

Q

-

S

-

G

-

K

K

G

G

I

V

A

A

Y

Y

L

I

L

M

E

K

A

T

E

D

Y

H

G

G

L

L

V

S

M

L

P

P

R

R

L

L

Q

Q

V

I

E

E

F

F

S

S

A

Q

A

V

I

I

Q

Q

R

K

I

V

T

-

D

-

E

-

R

-

G

-

T

-

E

-

L

-

S

S

A

P

G

K

D

E

I

M

W

W

R

D

A

A

F

F

E

A

E

E

Y

Y

L

L

A

A

V

P

A

V

A

R

P

P

Y

L

A

E

Y

R

V

I

E

R

H

Q

H

H

L

V

-

D

-

A

A

D

E

D

H

D

G

G

Q

T

G

S

I

I

S

T

L

A

T

T

V

V

E

K

E

I

A

N

G

G

V

V

R

E

R

T

V

E

L

I

R

S

G

G

V

S

G

G

N

N

G
|
G

P
|
P

I
x
L

D
x
A

A

A

A

F

L

V

H

H

A

A

L

L

-

A

D

D

A

V

G

G

-

F

-

D

A

V

V

A

L

V

L

L

G

D

Y

Y

E

Y

E

E

R

H

A

A

I

M

G

S

Q

A

G

G

G
x
D

D

D

A
|
A

R

Q

A

A

V

A

A

A

Y

Y

I

V

E

E

L

A

A

S

D

V

G

T

E

I

G

A

G

T

G

S

S

K

T

T

-

V

F

W

G

G

V

V

G

G

I

I

H

A

A
x
P

N

S

I
|
I

V

T

T

T

A

A

S

S

V

L

L

R

A

A

I

V

V

V

S

S

A

A

L

V

D

N

R

R

L

A

A

A

Q

R

binding leucine: G501 (= G484), P502 (= P485), L503 (≠ I486), A504 (≠ D487), D531 (≠ G511), A533 (= A513), P558 (≠ A537), I560 (= I539)
binding zinc ion: D64 (= D48), H268 (= H252), H270 (= H254)

3hpzB Crystal structure of mycobacterium tuberculosis leua complexed with bromopyruvate
57% identity, 96% coverage: 5:555/572 of query aligns to 25:576/576 of 3hpzB

query
sites
3hpzB

P

P

H

V

T

N

K

R

Y

Y

R

R

P

P

A

-

N

-

P

-

F

F

A

A

G

E

V

E

V

V

P

E

G

P

G

I

R

R

G

-

L

L

S

R

D

N

R

R

T

T

W

W

P

P

D

D

N

R

T

V

I

I

T

D

R

R

P

A

P

P

V

L

W

W

M

C

S

A

T

V

D

D

L

L

R

R

D
|
D

G

G

N

N

Q

Q

A

A

L

L

F

I

E

D

P

P

M

M

N

S

S

P

E

A

R

R

K

K

M

R

R

R

M

M

F

F

K

D

M

L

L

L

V

V

E

R

I

M

G

G

L

Y

K

K

E

E

I

I

E

E

V

V

A

G

F

F

P

P

A

S

A

A

S

S

Q

Q

T

T

D

D

F

F

D

D

F

F

V

V

R

R

E

E

L

I

I

I

E

E

G

Q

G

G

H

A

I

I

P

P

D

D

V

V

T

T

I

I

E

Q

V

V

L

L

T

T

Q

Q

A

C

R

R

P

P

H

E

L

L

I

I

E

E

R

R

T

T

F

F

E

Q

S

A

V

C

R

S

G

G

A

A

K

P

R

R

A

A

I

I

V

V

H

H

V

F

Y

Y

N

N

A

S

V

T

A

S

P

I

N

L

F

Q

R

R

I

V

V

V

F

F

D

R

T

A

D

N

R

R

A

A

G

E

V

V

K

Q

Q

A

I

I

A

A

V

T

D

D

S

G

A

A

R

R

Y

K

F

C

V

V

E

E

M

Q

A

A

R

K

Q

Y

P

P

G

G

T

T

D

Q

F

W

T

R

F

F

Q

E

Y

Y

S

S

P

P

E
|
E

V

S

F

Y

S

T

G

G

T

T

E

E

L

L

D

E

F

Y

A

A

V

K

E

Q

V

V

A

C

N

D

A

A

V

V

I

G

E

E

V

V

W

I

Q

A

P

P

T

T

P

P

Q

E

Q

R

K

P

C

I

I

I

I

F

N

N

L

L

P
|
P

A

A

T
|
T

V

V

E

E

M

M

S

T

T

T

P

P

N

N

V

V

Y

Y

A

A

D

D

Q

S

I

I

E

E

W

W

M

M

H

S

R

R

H

N

L

L

A

A

R

N

R

R

D

E

S

S

V

V

L

I

L

L

S

S

V

L

H
|
H

P

P

H
|
H

N

N

D

D

R

R

G

G

T

T

A

A

V

V

A

A

E

E

L

L

A

G

V

F

M

A

A

A

G

G

A

A

D

D

R

R

V

I

E

E

G

G

C

C

L

L

F

F

G

G

N

N

G

G

E

E

R

R

T

T

G

G

N

N

V

V

D

C

L

L

V

V

T

T

L

L

A

G

L

L

N

N

L

L

Y

F

S

S

Q

R

G

G

V

V

H

D

P

P

G

Q

L

I

D

D

F

F

S

S

R

N

I

I

N

D

E

E

V

I

A

R

R

R

T

T

V

V

E

E

H

Y

C

C

T

N

Q

Q

L

L

P

P

I

V

H

H

P

E

R

R

H

H

P

P

Y

Y

V

G

G

G

D

D

L

L

V

V

F

Y

T

T

A

A

F

F

S

S

G

G

S

S

H

H

Q

Q

D

D

A

A

I

I

K

N

K

K

G

G

F

L

A

D

V

A

Q

M

Q

K

-

L

-

D

-

A

-

D

-

A

-

D

-

C

-

D

-

V

P

D

D

D

A

M

V

L

W

W

E

Q

V

V

P

P

Y

Y

L

L

P

P

V

I

D

D

P

P

A

R

D

D

L

V

G

G

R

R

S

T

Y

Y

E

E

S

A

I

V

I

I

R

R

V

V

N

N

S

-

Q

-

S

-

G

-

K

K

G

G

I

V

A

A

Y

Y

L

I

L

M

E

K

A

T

E

D

Y

H

G

G

L

L

V

S

M

L

P

P

R

R

L

L

Q

Q

V

I

E

E

F

F

S

S

A

Q

A

V

I

I

Q

Q

R

K

I

V

T

-

D

-

E

-

R

-

G

-

T

-

E

-

L

-

S

S

A

P

G

K

D

E

I

M

W

W

R

D

A

A

F

F

E

A

E

E

Y

Y

L

L

A

A

V

P

A

V

A

R

P

P

Y

L

A

E

Y

R

V

I

E

R

H

Q

H

H

L

V

-

D

-

A

A

D

E

D

H

D

G

G

Q

T

G

S

I

I

S

T

L

A

T

T

V

V

E

K

E

I

A

N

G

G

V

V

R

E

R

T

V

E

L

I

R

S

G

G

V

S

G

G

N

N

G

G

P

P

I

L

D

A

A

A

A

F

L

V

H

H

A

A

L

L

-

A

D

D

A

V

G

G

-

F

-

D

A

V

V

A

L

V

L

L

G

D

Y

Y

E

Y

E

E

R

H

A

A

I

M

G

S

Q

A

G

G

G

D

D

D

A

A

R

Q

A

A

V

A

A

A

Y

Y

I

V

E

E

L

A

A

S

D

V

G

T

E

I

G

A

G

T

G

S

S

K

T

T

-

V

F

W

G

G

V

V

G

G

I

I

H

A

A

P

N

S

I

I

V

T

T

T

A

A

S

S

V

L

L

R

A

A

I

V

V

V

S

S

A

A

L

V

D

N

R

R

L

A

A

A

binding Bromopyruvate: E201 (= E185), P235 (= P219), T237 (= T221), H268 (= H252), H270 (= H254)
binding zinc ion: D64 (= D48), H268 (= H252), H270 (= H254)

3hq1A Crystal structure of mycobacterium tuberculosis leua complexed with citrate and mn2+
56% identity, 96% coverage: 5:555/572 of query aligns to 25:573/573 of 3hq1A

query
sites
3hq1A

P

P

H

V

T

N

K

R

Y

Y

R

R

P

P

A

-

N

-

P

-

F

F

A

A

G

E

V

E

V

V

P

E

G

P

G

I

R

R

G

-

L

L

S

R

D

N

R

R

T

T

W

W

P

P

D

D

N

R

T

V

I

I

T

D

R

R

P

A

P

P

V

L

W

W

M

C

S

A

T

V

D

D

L

L

R

R

D
|
D

G

G

N

N

Q

Q

A

A

L

L

F

I

E

D

P

P

M

M

N

S

S

P

E

A

R

R

K

K

M

R

R

R

M

M

F

F

K

D

M

L

L

L

V

V

E

R

I

M

G

G

L

Y

K

K

E

E

I

I

E

E

V

V

A

G

F

F

P

P

A

S

A

A

S

S

Q

Q

T

T

D

D

F

F

D

D

F

F

V

V

R

R

E

E

L

I

I

I

E

E

G

Q

G

G

H

A

I

I

P

P

D

D

V

V

T

T

I

I

E

Q

V

V

L

L

T

T

Q

Q

A

C

R

R

P

P

H

E

L

L

I

I

E

E

R

R

T

T

F

F

E

Q

S

A

V

C

R

S

G

G

A

A

K

P

R

R

A

A

I

I

V

V

H

H

V

F

Y

Y

N

N

A

S

V

T

A

S

P

I

N

L

F

Q

R

R

I

V

V

V

F

F

D

R

T

A

D

N

R

R

A

A

G

E

V

V

K

Q

Q

A

I

I

A

A

V

T

D

D

S

G

A

A

R

R

Y

K

F

C

V

V

E

E

M

Q

A

A

R

K

Q

Y

P

P

G

G

T

T

D

Q

F

W

T

R

F

F

Q

E

Y

Y

S

S

P

P

E

E

V

S

F

Y

S

T

G

G

T

T

E

E

L

L

D

E

F

Y

A

A

V

K

E

Q

V

V

A

C

N

D

A

A

V

V

I

G

E

E

V

V

W

I

Q

A

P

P

T

T

P

P

Q

E

Q

R

K

P

C

I

I

I

I

F

N

N

L

L

P

P

A

A

T

T

V

V

E

E

M

M

S

T

T

T

P

P

N

N

V

V

Y

Y

A

A

D

D

Q

S

I

I

E

E

W

W

M

M

H

S

R

R

H

N

L

L

A

A

R

N

R

R

D

E

S

S

V

V

L

I

L

L

S

S

V

L

H
|
H

P

P

H
|
H

N

N

D

D

R

R

G

G

T

T

A

A

V

V

A

A

E

E

L

L

A

G

V

F

M

A

A

A

G

G

A

A

D

D

R

R

V

I

E

E

G

G

C

C

L

L

F

F

G

G

N

N

G

G

E

E

R

R

T

T

G

G

N
|
N

V

V

D

C

L

L

V

V

T

T

L

L

A

G

L

L

N

N

L

L

Y

F

S

S

Q

R

G

G

V

V

H

D

P

P

G

Q

L

I

D

D

F

F

S

S

R

N

I

I

N

D

E

E

V

I

A

R

R

R

T

T

V

V

E

E

H

Y

C

C

T

N

Q

Q

L

L

P

P

I

V

H

H

P

E

R

R

H

H

P

P

Y

Y

V

G

G

G

D

D

L

L

V

V

F

Y

T

T

A

A

F

F

S

S

G

G

S

S

H

H

Q

Q

D

D

A

A

I

I

K

N

K

K

G

G

F

L

A

D

V

A

Q

M

Q

K

-

L

-

D

-

A

-

D

-

A

-

D

-

C

-

D

-

V

P

D

D

D

A

M

V

L

W

W

E

Q

V

V

P

P

Y

Y

L

L

P

P

V

I

D

D

P

P

A

R

D

D

L

V

G

G

R

R

S

T

Y

Y

E

E

S

A

I

V

I

I

R

-

V

-

N

-

S

-

Q

-

S

-

G

-

K

K

G

G

I

V

A

A

Y

Y

L

I

L

M

E

K

A

T

E

D

Y

H

G

G

L

L

V

S

M

L

P

P

R

R

L

L

Q

Q

V

I

E

E

F

F

S

S

A

Q

A

V

I

I

Q

Q

R

K

I

I

T

-

D

-

E

-

R

-

G

-

T

-

E

E

L

V

S

S

A

P

G

K

D

E

I

M

W

W

R

D

A

A

F

F

E

A

E

E

Y

Y

L

L

A

A

V

P

A

V

A

R

P

P

Y

L

A

E

Y

R

V

I

E

R

H

Q

H

H

L

V

-

D

-

A

A

D

E

D

H

D

G

G

Q

T

G

S

I

I

S

T

L

A

T

T

V

V

E

K

E

I

A

N

G

G

V

V

R

E

R

T

V

E

L

I

R

S

G

G

V

S

G

G

N

N

G

G

P

P

I

L

D

A

A

A

A

F

L

V

H

H

A

A

L

L

-

A

D

D

A

V

G

G

-

F

-

D

A

V

V

A

L

V

L

L

G

D

Y

Y

E

Y

E

E

R

H

A

A

I

M

G

S

Q

A

G

G

G

D

D

D

A

A

R

Q

A

A

V

A

A

A

Y

Y

I

V

E

E

L

-

A

A

D

S

G

V

E

T

G

I

G

S

G

K

S

T

T

V

F

W

G

G

V

V

G

G

I

I

H

A

A

P

N

S

I

I

V

T

T

T

A

A

S

S

V

L

L

R

A

A

I

V

V

V

S

S

A

A

L

V

D

N

R

R

L

A

A

A

binding manganese (ii) ion: D64 (= D48), H268 (= H252), H270 (= H254), N304 (= N288)

1sr9A Crystal structure of leua from mycobacterium tuberculosis (see paper)
56% identity, 96% coverage: 5:555/572 of query aligns to 25:573/573 of 1sr9A

query
sites
1sr9A

P

P

H

V

T

N

K

R

Y

Y

R

R

P

P

A

-

N

-

P

-

F

F

A

A

G

E

V

E

V

V

P

E

G

P

G

I

R

R

G

-

L

L

S

R

D

N

R

R

T

T

W

W

P

P

D

D

N

R

T

V

I

I

T

D

R

R

P

A

P

P

V

L

W

W

M

C

S

A

T

V

D

D

L

L

R
|
R

D
|
D

G

G

N

N

Q

Q

A

A

L

L

F

I

E

D

P

P

M

M

N

S

S

P

E

A

R

R

K

K

M

R

R

R

M

M

F

F

K

D

M

L

L

L

V

V

E

R

I

M

G

G

L

Y

K

K

E

E

I

I

E

E

V

V

A

G

F

F

P

P

A

S

A

A

S

S

Q

Q

T

T

D

D

F

F

D

D

F

F

V

V

R

R

E

E

L

I

I

I

E

E

G

Q

G

G

H

A

I

I

P

P

D

D

V

V

T

T

I

I

E

Q

V

V

L

L

T

T

Q

Q

A

C

R

R

P

P

H

E

L

L

I

I

E

E

R

R

T

T

F

F

E

Q

S

A

V

C

R

S

G

G

A

A

K

P

R

R

A

A

I

I

V

V

H

H

V

F

Y

Y

N

N

A

S

V

T

A

S

P

I

N

L

F

Q

R

R

I

V

V

V

F

F

D

R

T

A

D

N

R

R

A

A

G

E

V

V

K

Q

Q

A

I

I

A

A

V

T

D

D

S

G

A

A

R

R

Y

K

F

C

V

V

E

E

M

Q

A

A

R

K

Q

Y

P

P

G

G

T

T

D

Q

F

W

T

R

F

F

Q

E

Y

Y

S

S

P

P

E

E

V

S

F

Y

S

T

G

G

T

T

E

E

L

L

D

E

F

Y

A

A

V

K

E

Q

V

V

A

C

N

D

A

A

V

V

I

G

E

E

V

V

W

I

Q

A

P

P

T

T

P

P

Q

E

Q

R

K

P

C

I

I

I

I

F

N

N

L

L

P
|
P

A

A

T
|
T

V

V

E

E

M

M

S

T

T

T

P

P

N

N

V

V

Y

Y

A

A

D

D

Q

S

I

I

E

E

W

W

M

M

H

S

R

R

H

N

L

L

A

A

R

N

R

R

D

E

S

S

V

V

L

I

L

L

S

S

V

L

H
|
H

P

P

H
|
H

N

N

D

D

R

R

G

G

T

T

A

A

V

V

A

A

E

E

L

L

A

G

V

F

M

A

A

A

G

G

A

A

D

D

R

R

V

I

E

E

G

G

C

C

L

L

F

F

G

G

N

N

G

G

E

E

R

R

T

T

G

G

N

N

V

V

D

C

L

L

V

V

T

T

L

L

A

G

L

L

N

N

L

L

Y

F

S

S

Q

R

G

G

V

V

H

D

P

P

G

Q

L

I

D

D

F

F

S

S

R

N

I

I

N

D

E

E

V

I

A

R

R

R

T

T

V

V

E

E

H

Y

C

C

T

N

Q

Q

L

L

P

P

I

V

H

H

P

E

R

R

H

H

P

P

Y

Y

V

G

G

G

D

D

L

L

V

V

F

Y

T

T

A

A

F

F

S

S

G

G

S

S

H

H

Q

Q

D

D

A

A

I

I

K

N

K

K

G

G

F

L

A

D

V

A

Q

M

Q

K

-

L

-

D

-

A

-

D

-

A

-

D

-

C

-

D

-

V

P

D

D

D

A

M

V

L

W

W

E

Q

V

V

P

P

Y

Y

L

L

P

P

V

I

D

D

P

P

A

R

D

D

L

V

G

G

R

R

S

T

Y

Y

E

E

S

A

I

V

I

I

R

-

V

-

N

-

S

-

Q

-

S

-

G

-

K

K

G

G

I

V

A

A

Y

Y

L

I

L

M

E

K

A

T

E

D

Y

H

G

G

L

L

V

S

M

L

P

P

R

R

L

L

Q

Q

V

I

E

E

F

F

S

S

A

Q

A

V

I

I

Q

Q

R

K

I

I

T

-

D

-

E

-

R

-

G

-

T

-

E

E

L

V

S

S

A

P

G

K

D

E

I

M

W

W

R

D

A

A

F

F

E

A

E

E

Y

Y

L

L

A

A

V

P

A

V

A

R

P

P

Y

L

A

E

Y

R

V

I

E

R

H

Q

H

H

L

V

-

D

-

A

A

D

E

D

H

D

G

G

Q

T

G

S

I

I

S

T

L

A

T

T

V

V

E

K

E

I

A

N

G

G

V

V

R

E

R

T

V

E

L

I

R

S

G

G

V

S

G

G

N

N

G

G

P

P

I

L

D

A

A

A

A

F

L

V

H

H

A

A

L

L

-

A

D

D

A

V

G

G

-

F

-

D

A

V

V

A

L

V

L

L

G

D

Y

Y

E

Y

E

E

R

H

A

A

I

M

G

S

Q

A

G

G

G

D

D

D

A

A

R

Q

A

A

V

A

A

A

Y

Y

I

V

E

E

L

-

A

A

D

S

G

V

E

T

G

I

G

S

G

K

S

T

T

V

F

W

G

G

V

V

G

G

I

I

H

A

A

P

N

S

I

I

V

T

T

T

A

A

S

S

V

L

L

R

A

A

I

V

V

V

S

S

A

A

L

V

D

N

R

R

L

A

A

A

binding 3-methyl-2-oxobutanoic acid: R63 (= R47), P235 (= P219), T237 (= T221), H268 (= H252), H270 (= H254)
binding zinc ion: D64 (= D48), H268 (= H252), H270 (= H254)

3hpsA Crystal structure of mycobacterium tuberculosis leua complexed with ketoisocaproate (kic)
56% identity, 96% coverage: 5:555/572 of query aligns to 25:575/575 of 3hpsA

query
sites
3hpsA

P

P

H

V

T

N

K

R

Y

Y

R

R

P

P

A

-

N

-

P

-

F

F

A

A

G

E

V

E

V

V

P

E

G

P

G

I

R

R

G

-

L

L

S

R

D

N

R

R

T

T

W

W

P

P

D

D

N

R

T

V

I

I

T

D

R

R

P

A

P

P

V

L

W

W

M

C

S

A

T

V

D

D

L

L

R
|
R

D
|
D

G

G

N

N

Q

Q

A

A

L

L

F

I

E

D

P

P

M

M

N

S

S

P

E

A

R

R

K

K

M

R

R

R

M

M

F

F

K

D

M

L

L

L

V

V

E

R

I

M

G

G

L

Y

K

K

E

E

I

I

E

E

V

V

A

G

F

F

P

P

A

S

A

A

S

S

Q

Q

T

T

D

D

F

F

D

D

F

F

V

V

R

R

E

E

L

I

I

I

E

E

G

Q

G

G

H

A

I

I

P

P

D

D

V

V

T

T

I

I

E

Q

V

V

L

L

T

T

Q

Q

A

C

R

R

P

P

H

E

L

L

I

I

E

E

R

R

T

T

F

F

E

Q

S

A

V

C

R

S

G

G

A

A

K

P

R

R

A

A

I

I

V

V

H
|
H

V

F

Y
|
Y

N

N

A

S

V

T

A

S

P

I

N

L

F

Q

R

R

I

V

V

V

F

F

D

R

T

A

D

N

R

R

A

A

G

E

V

V

K

Q

Q

A

I

I

A

A

V

T

D

D

S

G

A

A

R

R

Y

K

F

C

V

V

E

E

M

Q

A

A

R

K

Q

Y

P

P

G

G

T

T

D

Q

F

W

T

R

F

F

Q

E

Y

Y

S

S

P

P

E

E

V

S

F

Y

S

T

G

G

T

T

E

E

L

L

D

E

F

Y

A

A

V

K

E

Q

V

V

A

C

N

D

A

A

V

V

I

G

E

E

V

V

W

I

Q

A

P

P

T

T

P

P

Q

E

Q

R

K

P

C

I

I

I

I

F

N

N

L

L

P
|
P

A

A

T
|
T

V

V

E

E

M

M

S

T

T

T

P

P

N

N

V

V

Y

Y

A

A

D

D

Q

S

I

I

E

E

W

W

M

M

H

S

R

R

H

N

L

L

A

A

R

N

R

R

D

E

S

S

V

V

L

I

L

L

S

S

V

L

H
|
H

P

P

H
|
H

N

N

D

D

R

R

G

G

T

T

A

A

V

V

A

A

E

E

L

L

A

G

V

F

M

A

A

A

G

G

A

A

D

D

R

R

V

I

E

E

G

G

C

C

L

L

F

F

G

G

N

N

G

G

E

E

R

R

T

T

G

G

N

N

V

V

D

C

L

L

V

V

T

T

L

L

A

G

L

L

N

N

L

L

Y

F

S

S

Q

R

G

G

V

V

H

D

P

P

G

Q

L

I

D

D

F

F

S

S

R

N

I

I

N

D

E

E

V

I

A

R

R

R

T

T

V

V

E

E

H

Y

C

C

T

N

Q

Q

L

L

P

P

I

V

H

H

P

E

R

R

H

H

P

P

Y

Y

V

G

G

G

D

D

L

L

V

V

F

Y

T

T

A

A

F

F

S

S

G

G

S

S

H

H

Q

Q

D

D

A

A

I

I

K

N

K

K

G

G

F

L

A

D

V

A

Q

M

Q

K

-

L

-

D

-

A

-

D

-

A

-

D

-

C

-

D

-

V

P

D

D

D

A

M

V

L

W

W

E

Q

V

V

P

P

Y

Y

L

L

P

P

V

I

D

D

P

P

A

R

D

D

L

V

G

G

R

R

S

T

Y

Y

E

E

S

A

I

V

I

I

R

-

V

-

N

-

S

-

Q

-

S

-

G

-

K

K

G

G

I

V

A

A

Y

Y

L

I

L

M

E

K

A

T

E

D

Y

H

G

G

L

L

V

S

M

L

P

P

R

R

L

L

Q

Q

V

I

E

E

F

F

S

S

A

Q

A

V

I

I

Q

Q

R

K

I

I

T

-

D

-

E

-

R

-

G

-

T

-

E

E

L

V

S

S

A

P

G

K

D

E

I

M

W

W

R

D

A

A

F

F

E

A

E

E

Y

Y

L

L

A

A

V

P

A

V

A

R

P

P

Y

L

A

E

Y

R

V

I

E

R

H

Q

H

H

L

V

-

D

-

A

A

D

E

D

H

D

G

G

Q

T

G

S

I

I

S

T

L

A

T

T

V

V

E

K

E

I

A

N

G

G

V

V

R

E

R

T

V

E

L

I

R

S

G

G

V

S

G

G

N

N

G
|
G

P
|
P

I
x
L

D
x
A

A

A

A

F

L

V

H

H

A

A

L

L

-

A

D

D

A

V

G

G

-

F

-

D

A

V

V

A

L

V

L

L

G

D

Y

Y

E

Y

E

E

R

H

A

A

I

M

G

S

Q

A

G

G

G
x
D

D

D

A
|
A

R
x
Q

A

A

V

A

A

A

Y

Y

I

V

E

E

L

A

A

S

D

V

G

T

E

I

G

A

G

T

G

S

S

K

T

T

-

V

F

W

G

G

V

V

G

G

I

I

H

A

A
x
P

N

S

I
|
I

V

T

T

T

A

A

S

S

V

L

L

R

A

A

I

V

V

V

S

S

A

A

L

V

D

N

R

R

L

A

A

A

binding 2-oxo-4-methylpentanoic acid: R63 (= R47), H150 (= H134), Y152 (= Y136), P235 (= P219), T237 (= T221), H268 (= H252), H270 (= H254)
binding leucine: G500 (= G484), P501 (= P485), L502 (≠ I486), A503 (≠ D487), D530 (≠ G511), A532 (= A513), Q533 (≠ R514), P557 (≠ A537), I559 (= I539)
binding zinc ion: D64 (= D48), H268 (= H252), H270 (= H254)

4ov4A Isopropylmalate synthase binding with ketoisovalerate (see paper)
28% identity, 68% coverage: 46:434/572 of query aligns to 11:374/379 of 4ov4A

query
sites
4ov4A

L

L

R
|
R

D
|
D

G

G

N

N

Q
|
Q

A

A

L

L

F

K

E

R

P

P

M

W

N

T

S

I

E

D

R

E

K

K

M

I

R

E

M

V

F

F

K

D

M

L

L

L

V

V

E

E

I

L

G

N

L

V

K

D

E

G

I

I

E

E

V

V

A

G

F

F

P

P

A

S

S

N

Q

E

T

T

D

E

F

F

D

H

F

T

V

C

R

Q

E

V

L

L

I

-

E

-

G

S

G

K

H

R

I

A

P

P

D

K

D

G

V

K

T

P

I

I

E

A

V

A

L

L

T

S

Q

R

A

A

R

N

P

Q

H

N

L

E

I

I

E

A

R

V

T

T

F

W

E

E

S

A

V

I

R

Q

G

K

A

A

K

D

R

C

A

P

I

R

V

M

H

H

V

I

Y

V

N

Y

A

P

V

V

A

S

P

D

-

F

N

S

F

I

R

K

R

H

I

V

V

L

F

K

D

I

T

S

D

E

R

K

A

E

G

V

V

L

K

Q

Q

K

I

I

A

-

V

-

D

-

S

-

A

-

R

R

Y

N

F

S

V

I

E

S

M

F

A

A

A

R

R

S

Q

I

P

V

G

G

T

P

D

G

F

I

T

E

F

I

Q

Q

Y

F

S

S

P

G

E

E

V

H

F

F

S

G

G

D

T

A

E

I

L

E

D

N

F

F

A

A

V

F

E

-

V

T

A

K

N

E

A

A

V

F

I

L

E

T

V

A

W

I

Q

E

P

A

T

G

P

A

Q

N

Q

-

K

-

C

-

I

I

N

N

L

L

P
|
P

A

N

T
|
T

V

V

E

E

M

R

S

Y

T

R

P

P

N

M

V

V

Y

F

A

V

D

N

Q

M

I

V

E

K

W

E

M

I

H

K

R

D

H

V

L

V

A

-

R

-

R

K

D

D

S

K

V

A

L

I

S

S

V

I

H
|
H

P

T

H
|
H

N

N

D

D

R

L

G

G

T

M

A

A

V

T

A

A

A

T

A

S

E

V

L

E

A

S

V

V

M

Y

A

V

G

G

A

A

D

E

R

Q

V

I

E

E

G

V

C

A

L

L

F

N

G

G

N

L

G

G

E

E

R

R

T

A

G

G

N

N

V

T

D

N

L

L

V

Y

T

E

L

T

A

A

L

I

N

A

L

L

Y

H

S

Q

Q

N

G

G

V

E

H

N

P

L

G

N

L

I

D

N

F

F

S

Q

R

R

I

I

N

Y

E

P

V

T

A

A

R

K

T

R

V

I

E

S

H

E

C

L

T

T

Q

G

L

I

P

P

I

I

H

G

P

E

R

K

H

T

P

P

Y

I

V

I

G

G

D

E

L

D

V

I

F

F

T

S

A

H

F

R

S

S

G

G

S

I

H

H

Q

Q

D

H

A

Q

I

S

K

K

K

G

G

A

F

Y

A

R

V

T

Q

F

Q

S

P

P

D

E

A

F

V

V

W

-

E

-

V

-

P

-

Y

-

L

-

P

-

V

-

D

-

P

-

A

-

D

-

L

-

G

G

R

R

S

M

Y

D

E

K

S

E

I

T

I

I

R

S

V

F

N

T

S

N

Q

Q

S

S

G

G

K

H

G

K

G

A

I

I

A

E

Y

F

L

L

E

H

A

Q

E

R

Y

G

G

I

L

Q

V

V

M

S

P

K

R

E

R

G

L

I

Q

H

V

H

E

L

F

F

S

S

A

L

A

A

I

K

Q

S

R

I

I

S

T

S

D

R

E

E

R

N

G

N

T

R

E

E

L

I

S

T

A

E

G

A

D

E

I

L

active site: Q16 (= Q51)
binding 3-methyl-2-oxobutanoic acid: R12 (= R47), P174 (= P219), T176 (= T221), H205 (= H252), H207 (= H254)
binding zinc ion: D13 (= D48), H205 (= H252), H207 (= H254)

4ov9A Structure of isopropylmalate synthase binding with alpha- isopropylmalate (see paper)
28% identity, 68% coverage: 46:434/572 of query aligns to 11:376/380 of 4ov9A

query
sites
4ov9A

L

L

R
|
R

D
|
D

G

G

N

N

Q
|
Q

A

A

L

L

F

K

E

R

P

P

M

W

N

T

S

I

E

D

R

E

K

K

M

I

R

E

M

V

F

F

K

D

M

L

L

L

V

V

E

E

I

L

G

N

L

V

K

D

E

G

I

I

E

E

V

V

A

G

F

F

P

P

A

S

S

N

Q

E

T

T

D

E

F

F

D

H

F

T

V

C

R

Q

E

V

L

L

I

-

E

-

G

S

G

K

H

R

I

A

P

P

D

K

D

G

V

K

T

P

I

I

E

A

V

A

L
|
L

T

S

Q

R

A

A

R

N

P

Q

H

N

L

E

I

I

E

A

R

V

T

T

F

W

E

E

S

A

V

I

R

Q

G

K

A

A

K

D

R

C

A

P

I

R

V

M

H

H

V

I

Y

V

N

Y

A

P

V

V

A

S

P

D

-

F

N

S

F

I

R

K

R

H

I

V

V

L

F

K

D

I

T

S

D

E

R

K

A

E

G

V

V

L

K

Q

Q

K

I

I

A

-

V

-

D

-

S

-

A

-

R

R

Y

N

F

S

V

I

E

S

M

F

A

A

A

R

R

S

Q

I

P

V

G

G

T

P

D

G

F

I

T

E

F

I

Q

Q

Y

F

S

S

P

G

E

E

V

H

F

F

S

G

G

D

T

A

E

I

L

E

D

N

F

F

A

A

V

F

E

-

V

T

A

K

N

E

A

A

V

F

I

L

E

T

V

A

W

I

Q

E

P

A

T

G

P

A

Q

N

Q

-

K

-

C

-

I

I

N

N

L

L

P

P

A

N

T
|
T

V

V

E

E

M

R

S

Y

T

R

P

P

N

M

V

V

Y

F

A

V

D

N

Q

M

I

V

E

K

W

E

M

I

H

K

R

D

H

V

L

V

A

-

R

-

R

K

D

D

S

K

V

A

L

I

S

S

V

I

H
|
H

P

T

H
|
H

N

N

D

D

R

L

G

G

T

M

A

A

V

T

A

A

A

T

A

S

E

V

L

E

A

S

V

V

M

Y

A

V

G

G

A

A

D

E

R

Q

V

I

E

E

G

V

C

A

L

L

F

N

G

G

N

L

G

G

E

E

R

R

T

A

G

G

N

N

V

T

D

N

L

L

V

Y

T

E

L

T

A

A

L

I

N

A

L

L

Y

H

S

Q

Q

N

G

G

V

E

H

N

P

L

G

N

L

I

D

N

F

F

S

Q

R

R

I

I

N

Y

E

P

V

T

A

A

R

K

T

R

V

I

E

S

H

E

C

L

T

T

Q

G

L

I

P

P

I

I

H

G

P

E

R

K

H

T

P

P

Y

I

V

I

G

G

D

E

L

D

V

I

F

F

T

S

A

H

F

R

S

S

G

G

S

I

H

H

Q

Q

D

-

A

-

I

-

K

-

G

-

F

-

A

T

V

L

Q

H

Q

Q

P

S

D

K

A

G

V

A

W

-

E

-

V

-

P

-

Y

Y

L

R

P

T

V

F

D

S

P

P

A

E

D

F

L

V

G

G

R

R

S

M

Y

D

E

K

S

E

I

T

I

I

R

S

V

F

N

T

S

N

Q

Q

S

S

G

G

K

H

G

K

G

A

I

I

A

E

Y

F

L

L

E

H

A

Q

E

R

Y

G

G

I

L

Q

V

V

M

S

P

K

R

E

R

G

L

I

Q

H

V

H

E

L

F

F

S

S

A

L

A

A

I

K

Q

S

R

I

I

S

T

S

D

R

E

E

R

N

G

N

T

R

E

E

L

I

S

T

A

E

G

A

D

E

I

L

active site: Q16 (= Q51)
binding (2S)-2-hydroxy-2-(propan-2-yl)butanedioic acid: R12 (= R47), L73 (= L110), T176 (= T221), H205 (= H252), H207 (= H254)
binding zinc ion: D13 (= D48), H205 (= H252), H207 (= H254)

Q9JZG1 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Neisseria meningitidis serogroup B (strain MC58) (see 2 papers)
27% identity, 93% coverage: 34:565/572 of query aligns to 2:515/517 of Q9JZG1

query
sites
Q9JZG1

T

T

I

Q

T

T

R

N

P

R

P

V

V

I

W

I

M

F

S

D

T

T

D

T

L

L

R

R

D
|
D

G

G

N

E

Q

Q

A

S

L

P

F

G

E

A

P

A

M

M

N

T

S

K

E

E

R

E

K

K

M

I

R

R

M

V

F

A

K

R

M

Q

L

L

V

E

E

K

I

L

G

G

L

V

K

D

E

I

I

I

E

E

V

A

A

G

F

F

P

A

A

A

S

S

Q

P

T

G

D

D

F

F

D

E

F

A

V

V

R

N

E

A

L

I

I

-

E

-

G

-

G

A

H

K

I

T

P

I

D

T

D

K

V

S

T

T

I

V

E

C

V

S

L

L

T

S

Q

R

A

A

R

I

P

E

H

R

L

D

I

I

E

R

R

Q

T

A

F

G

E

E

S

A

V

V

R

A

G

P

A

A

K

P

R

K

A

K

I

R

V

I

H

H

V

T

Y

F

N

I

A

A

V

T

A

S

P

P

N

I

F

H

R

M

R

E

I

Y

V

K

F

L

D

K

T

M

D

K

R

P

A

K

G

Q

V

V

K

I

Q

E

I

A

A

A

V

V

D

K

S

A

A

V

R

K

Y

I

F

-

V

-

E

-

M

-

A

-

A

A

R

R

Q

E

P

Y

G

T

T

D

D

D

F

V

T

E

F

F

Q

S

Y

C

S

E

P

D

E

A

V

L

F

R

S

S

G

-

T

-

E

E

L

I

D

D

F

F

A

L

V

A

E

E

V

I

A

C

N

G

A

A

V

V

I

I

E

E

V

A

W

G

Q

A

P

T

T

T

P

-

Q

-

K

-

C

-

I

-

I

I

N

N

L

I

P

P

A

D

T

T

V

V

E

G

M

Y

S

S

T

I

P

P

N

Y

V

-

Y

-

A

-

D

-

Q

K

I

T

E

E

W

E

M

F

H

F

R

R

H

E

L

L

A

I

R

A

R

K

D

T

-

P

-

N

-

G

S

K

V

V

L

V

L

W

S

S

V

A

H
|
H

P

C

H
|
H

N

N

D

D

R

L

G

G

T

L

A

A

V

V

A

A

A

N

A

S

E

L

L

A

A

A

V

L

M

K

A

G

G

G

A

A

D

R

R

Q

V

V

E

E

G

C

C

T

L

V

F

N

G

G

N

L

G

G

E

E

R

R

T

A

G

G

N
|
N

-

A

-

S

V

V

D

E

L

E

V

I

T

V

L

M

A

A

L

L

N

K

L

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Y

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Q

D

-

L

-

F

G

G

V

L

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E

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T

G

G

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I

D

D

F

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T

R

Q

I

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N

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E

P

V

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A

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R

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V

E

S

H

T

C

I

T

T

Q

G

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Y

P

P

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V

H

Q

P

P

R

N

H

K

P

A

Y

I

V

V

G

G

D

A

L

N

V

A

F

F

T

S

A

H

F

E

S

S

G

G

S

I

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H

Q

Q

D

D

A

G

I

V

K

L

K

K

-

H

-

R

-

E

G

T

F

Y

A

E

V

I

Q

M

Q

S

P

A

D

E

A

S

V

V

-

G

W

W

E

A

V

T

P

N

Y

R

L

L

P

-

V

-

D

-

P

-

A

-

D

S

L

L

G

G

R

K

S

-

Y

-

E

-

S

-

I

-

R

-

V

-

N

-

S

-

Q

L

S

S

G

G

K

R

G

N

G

A

I

F

A

K

Y

T

L

K

L

L

E

-

A

A

E

D

Y

L

G

G

L

I

V

E

M

L

P

E

R

S

R

-

L

-

Q

E

V

E

E

A

F

L

S

N

A

A

I

F

Q

A

R

R

I

F

T

K

D

E

-
x
L

-
x
A

E
x
D

R
x
K

G
x
K

T
x
R

E
|
E

L
x
I

S
x
F

A
x
D

G
x
E

D
|
D

I
x
L

W
x
H

R
x
A

A
x
L

F
x
V

E
x
S

E
x
D

E
|
E

Y
x
M

L
x
G

A
x
S

V
x
M

-
x
N

A
|
A

A
x
E

P
x
S

Y
|
Y

A
x
K

Y
x
F

V
x
I

E
x
S

H
x
Q

H
x
K

L
x
I

A
x
S

E
x
T

H
x
E

G
x
T

G
|
G

Q
x
E

-
x
P

-
x
R

-
x
A

G
x
D

I
|
I

S
x
V

L
x
F

T
x
S

V
x
I

E
x
K

E

-

A

-

G
|
G

V
x
E

R
x
E

R
x
K

V
x
R

L
x
A

R
x
S

G
x
A

V
x
T

G
|
G

N
x
S

G
|
G

P
|
P

I
x
V

D
|
D

A
|
A

A
x
I

L
x
F

H
x
K

A
|
A

L
x
I

D
x
E

-
x
S

-
x
V

-
x
A

-
x
Q

A
x
S

G
|
G

A
|
A

V
x
A

L
|
L

L
x
Q

G
x
I

Y
|
Y

E
x
S

E
x
V

R
x
N

A
|
A

I
x
V

G
x
T

Q
|
Q

G
|
G

G
x
T

D
x
E

A
x
S

R
x
Q

A
x
G

V
x
E

A
x
T

Y
x
S

I
x
V

E
x
R

L
|
L

A
|
A

D

-

G

-

E
x
R

G
|
G

G
x
N

G
x
R

S
x
V

T
x
V

F
x
N

G
|
G

V
x
Q

G
|
G

I
x
A

H
x
D

A
x
T

N
x
D

I
x
V

V
x
L

T
x
V

A
|
A

S
x
T

V
x
A

L
x
K

A
|
A

I
x
Y

V
x
L

S
|
S

A
|
A

L
|
L

D
x
S

R
x
K

L
|
L

-
x
E

-
x
F

A
x
S

Q
x
A

R
x
A

R
x
K

E
x
P

R
x
K

A
|
A

D
x
Q

G
|
G

V
x
S

G
|
G

D16 (= D48) binding Mn(2+)
H204 (= H252) binding Mn(2+)
H206 (= H254) binding Mn(2+)
N240 (= N288) binding Mn(2+)

Sites not aligning to the query:

366:517 Required for the condensation reaction. Not required to bind substrate

Q9FN52 Methylthioalkylmalate synthase 3, chloroplastic; 2-isopropylmalate synthase 2; Methylthioalkylmalate synthase-like; EC 2.3.3.17 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
25% identity, 62% coverage: 42:394/572 of query aligns to 88:432/503 of Q9FN52

query
sites
Q9FN52

M

L

S

D

T

T

D

T

L

L

R

R

D

D

G

G

N

E

Q

Q

A

S

L

P

F

G

E

A

P

A

M

L

N

T

S

P

E

P

R

Q

K

K

M

L

R

E

M

I

F

A

K

R

M

Q

L

L

V

A

E

K

I

L

G

R

L

V

K

D

E

I

I

M

E

E

V

V

A

G

F

F

P

P

A

V

A

S

S

S

Q

E

T

E

D

E

F

F

D

E

F

A

V

I

R

K

E

T

L

I

I

A

-

K

-

T

-

V

-

G

-

N

-

E

-

V

-

D

-

E

E

E

G

T

G

G

H

Y

I

V

P

P

D

-

V

-

T

V

I

I

E

C

V

G

L

I

T

A

Q

R

A

C

R

K

P

K

H

R

L

D

I

I

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E

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A

T

T

F

W

E

E

S

A

V

L

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K

G

Y

A

A

K

K

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V

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V

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K

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S

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Y

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K

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L

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A

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D

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H

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N

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G

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A

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Y

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G263 (≠ E223) mutation to E: In gsm2-1; loss of activity and lack of C6, C7 and C8 aliphatic glucosinolates.

6e1jA Crystal structure of methylthioalkylmalate synthase (bjumam1.1) from brassica juncea (see paper)
25% identity, 64% coverage: 31:394/572 of query aligns to 13:365/409 of 6e1jA

query
sites
6e1jA

P

P

D

D

N

K

T

N

I

Y

T

V

R

R

P

-

V

-

W

V

M

F

S

D

T

T

D

T

L

L

R

R

D
|
D

G

G

N

E

Q
|
Q

A

S

L

P

F

G

E

A

P

A

M

L

N

T

S

P

E

P

R

Q

K

K

M

I

R

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M

I

F

A

K

R

M

Q

L

L

V

A

E

K

I

L

G

R

L

V

K

D

E

I

I

M

E

E

V

V

A

G

F
|
F

P

P

A

V

A
x
S

S

S

Q

E

T

E

D

E

F

F

D

E

F

T

V

I

R

Q

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T

L

I

I

A

-

K

-

T

-

V

-

G

-

N

-

E

-
x
V

-

D

-
x
E

E

E

G

T

G

G

H

Y

I

I

P

P

D

-

V

-

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V

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I

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C

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V

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x
I

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A

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x
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A

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H

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I

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V

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Y

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A

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K

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V

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x
F

N

T

A

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A

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D

N

I

F

H

R

L

R

K

I

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K

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x
L

D

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M

D

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R

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G

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V

K

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S

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|
T

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D

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V

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|
H

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H
|
H

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N

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G

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A

A

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A

A

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A

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|
S

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|
G

S
x
I

H

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|
D

A

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|
K

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H

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binding coenzyme a: Q30 (= Q51), F60 (= F81), S63 (≠ A84), I95 (≠ L110), R97 (≠ Q112), F121 (≠ Y136), K132 (≠ V147), L133 (≠ F148), S322 (= S343), G323 (= G344), I324 (≠ S345), D327 (= D348), K331 (= K352), L359 (≠ Q388), R362 (≠ K391), H363 (≠ G392)
binding 4-(methylsulfanyl)-2-oxobutanoic acid: P192 (= P219), T194 (= T221), H225 (= H252), H227 (= H254)
binding manganese (ii) ion: D27 (= D48), V82 (vs. gap), E84 (vs. gap), H225 (= H252), H227 (= H254)

Q9FG67 Methylthioalkylmalate synthase 1, chloroplastic; 2-isopropylmalate synthase 3; EC 2.3.3.17 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
25% identity, 63% coverage: 30:391/572 of query aligns to 71:429/506 of Q9FG67

query
sites
Q9FG67

W

W

P

P

D

E

N

Y

T

I

I

P

T

N

R

K

P

L

P

P

-

D

-

G

-

N

-

Y

-

V

V

R

W

V

M

F

S

D

T

T

D

T

L

L

R

R

D

D

G

G

N

E

Q

Q

A

S

L

P

F

G

E

G

P
x
S

M

L

N

T

S

P

E

P

R

Q

K

K

M

L

R

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M

I

F

A

K

R

M

Q

L

L

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A

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K

I

L

G

R

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K

D

E

I

I

M

E

E

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V

A

G

F

F

P

P

A

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A

S

S

S

Q

E

T

E

D

E

F

L

D

E

F

T

V

I

R

K

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T

L

I

I

A

-

K

-

T

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V

-

G

-

N

-

E

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V

-

D

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E

E

E

G

T

G

G

H

Y

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P

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V

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V

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I

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A

L

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A

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A

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H

H

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I

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A

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W

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A

V

L

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K

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Y

A

A

K

K

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H

L

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V

Y

F

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T

A

S

V

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N

I

F

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M

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K

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L

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K

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K

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A

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V

K

I

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I

M

A

A

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V

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S

S

S

A

I

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Y

F

F

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V

-

E

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M

-

A

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A

A

R

K

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G

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F

I

Q

Q

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C

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Y

A

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L

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W

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L

H

K

R

A

H

N

L

T

A

P

R

G

R

I

D

D

S

D

V

V

L

V

S
x
A

V

V

H

H

P

C

H

H

N

N

D

D

R

L

G

G

T

L

A

A

V

T

A

A

A

N

A

S

E

I

L

A

A

G

V

I

M

R

A

A

G

G

A

A

D

R

R

Q

V

V

E

E

G

V

C

T

L

I

F

N

G

G

N

I

G

G

E

E

R

R

T

S

G

G

N

N

V

A

D

S

L

L

-

E

V

V

T

V

L

M

A

A

L

L

N

K

L

C

Y

R

S

G

-

A

-

Y

-

V

-

I

Q

N

G

G

V

V

H

Y

P

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K

L

I

D

D

F

T

S

R

R

Q

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I

N

M

E

A

V

T

A

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R

K

T

M

V

V

E

Q

H

E

C

Y

T

T

Q

G

L

L

P

Y

I

V

H

Q

P

A

R

H

H

K

P

P

Y

I

V

V

G

G

D

A

L

N

V

C

F

F

T

V

A

H

F

E

S

S

G

G

S

I

H

H

Q

Q

D

D

A

G

I

I

K

L

K

K

G

N

F

R

A

S

V

T

Q

-

P

-

D

-

A

-

V

-

W

-

E

-

V

-

P

-

Y

Y

L

E

P

I

V

L

D

S

P

P

A

E

D

D

L

I

G

G

-

I

-

V

R

K

S

S

Y

Q

E

N

S

S

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G

I

L

R

V

V

L

N

G

S

K

Q

L

S

S

G

G

K

R

S102 (≠ P56) mutation to F: In gsm1-1; loss of conversion of C3 to C4 glucosinolates.
A290 (≠ S250) mutation to T: In gsm1-2; loss of conversion of C3 to C4 glucosinolates.

3rmjB Crystal structure of truncated alpha-isopropylmalate synthase from neisseria meningitidis (see paper)
30% identity, 53% coverage: 40:340/572 of query aligns to 5:293/308 of 3rmjB

query
sites
3rmjB

V

I

W

I

M

F

S

D

T

T

D

T

L

L

R

R

D
|
D

G

G

N

E

Q
|
Q

A

S

L

P

F

G

E

A

P

A

M

M

N

T

S

K

E

E

R

E

K

K

M

I

R

R

M

V

F

A

K

R

M

Q

L

L

V

E

E

K

I

L

G

G

L

V

K

D

E

I

I

I

E

E

V

A

A

G

F

F

P

A

A

A

S

S

Q

P

T

G

D

D

F

F

D

E

F

A

V

V

R

N

E

A

L

I

I

-

E

-

G

-

G

A

H

K

I

T

P

I

D

T

D

K

V

S

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T

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V

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C

V

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L

L

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S

Q

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A

A

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P

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H

R

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D

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V

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A

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P

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A

K

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H

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F

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A

A

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F

H

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M

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A

A

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F

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V

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A

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A

A

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D

D

D

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F

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Y

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A

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D

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Q

K

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W

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F

H

F

R

R

H

E

L

L

A

I

R

A

R

K

D

T

-

P

-

N

-

G

S

K

V

V

L

V

L

W

S

S

V

A

H
|
H

P

C

H
|
H

N

N

D

D

R

L

G

G

T

L

A

A

V

V

A

A

A

N

A

S

E

L

L

A

A

A

V

L

M

K

A

G

G

G

A

A

D

R

R

Q

V

V

E

E

G

C

C

T

L

V

F

N

G

G

N

L

G

G

E

E

R

R

T

A

G

G

N
|
N

-

A

-

S

V

V

D

E

L

E

V

I

T

V

L

M

A

A

L

L

N

K

L

V

Y

R

S

H

Q

D

-

L

-

F

G

G

V

L

H

E

P

T

G

G

L

I

D

D

F

T

S

T

R

Q

I

I

N

V

E

P

V

S

A

S

R

K

T

L

V

V

E

S

H

T

C

I

T

T

Q

G

L

Y

P

P

I

V

H

Q

P

P

R

N

H

K

P

A

Y

I

V

V

G

G

D

A

L

N

V

A

F

F

T

S

active site: Q16 (= Q51)
binding manganese (ii) ion: D13 (= D48), H201 (= H252), H203 (= H254), N237 (= N288)

6ktqA Crystal structure of catalytic domain of homocitrate synthase from sulfolobus acidocaldarius (sahcs(dram)) in complex with alpha- ketoglutarate/zn2+/coa (see paper)
22% identity, 62% coverage: 42:394/572 of query aligns to 25:346/399 of 6ktqA

query
sites
6ktqA

M

L

S

D

T

S

D

T

L

L

R
|
R

D
x
E

G

G

N

E

Q

Q

A

T

L

P

F

G

E

V

P

V

M

F

N

T

S

T

E

D

R

Q

K

R

M

V

R

E

M

I

F

A

K

K

M

A

L

L

V

S

E

D

I

I

G

G

L

V

K

Q

E

M

I

I

E

E

V

A

A

G

F

H

P

P

A

A

A
x
V

S

S

Q

P

T

D

D

I

F

Y

D

E

F

G

V

I

R

R

E

R

L

I

I

I

E

K

G

-

H

-

I

L

P

K

D

R

D

E

V

G

T

V

I

I

E

K

V

S

L

E

T

I

Q

V

A

A

R

H

P

S

H
x
R

L
x
A

I

V

E

K

R

R

T

D

F

I

E

E

S

V

V

G

R

A

G

E

A

I

K

E

R

A

A

D

I

R

V

I

H

A

V

I

Y
x
F

N

Y

A

G

V

I

A

S

P

D

N

T

F

H

R

L

R

K

I

A

V

K

F
x
H

D

H

T

T

D

T

R

R

A

D

G

-

V

-

K

-

Q

-

I

-

A

-

V

-

D

E

S

A

A

L

R

R

Y

S

F

I

V

A

E

E

M

T

A

V

A

S

R

Y

Q

A

P

K

G

S

T

H

D

G

F

V

T

K

F

V

Q
x
R

Y

F

S
x
T

P

A

E
|
E

V

D

F

A

S

T

G

R

T

A

E

D

L

Y

D

Q

F

Y

A

L

V

L

E

E

V

V

A

I

N

K

A

T

V

V

I

R

E

D

V

A

W

G

Q

A

P

D

T

R

P

-

Q

-

K

-

C

-

I

-

I

V

N
x
S

L

I

P

A

A

D

T
|
T

V

V

E

G

M

V

S

-

T

-

P

-

N

-

V

L

Y

Y

A

P

D

S

Q

R

I

T

E

R

W

E

M

L

H

F

R

K

H

D

L

L

A

T

R

S

R

R

-

F

D

P

S

D

V

I

L

E

L

F

S

D

V

I

H
|
H

P

A

H
|
H

N

N

D

D

R

L

G

G

T

M

A

A

V

V

A

A

A

N

A

V

E

L

L

A

A

A

V

A

M

E

A

G

G

G

A

A

D

T

R

I

V

I

E

H

G

T

C

T

L

L

F

N

G

G

N

L

G

G

E

E

R

R

T

V

G

G

N

I

V

A

D

P

L

L

V

Q

T

V

L

V

A

A

L

A

N

A

L

L

-

K

Y

Y

S

H

Q

F

G

G

V

I

H

E

P

V

G

-

L

V

D

D

F

L

S

K

R

K

I

L

N

S

E

E

V

V

A

A

R

S

T

L

V

V

E

E

H

K

C

Y

T

S

Q

G

L

I

P

A

I

L

H

P

P

P

R

N

H

F

P

P

Y

I

V

T

G

G

D

D

L

Y

V

A

F

F

T

V

A

H

F

K

S

A

G

G

S

V

H

H

Q

V

D

A

A

G

I

V

K

-

G

-

F

-

A

-

V

L

Q

N

Q

D

P

P

D

-

A

K

V

T

W

Y

E

E

V

-

P

-

Y

F

L

L

P

P

V

-

D

-

P

P

A

E

D

T

L

F

G

G

R

R

S

S

Y

R

E

D

S

Y

I

V

I

I

R

-

V

-

N

D

S

K

Q

Y

S

T

G

G

K

K

G

H

G

A

I

V

binding 2-oxoglutaric acid: R30 (= R47), R154 (≠ Q181), T156 (≠ S183), E158 (= E185), S184 (≠ N217), T188 (= T221), H216 (= H252), H218 (= H254)
binding coenzyme a: V67 (≠ A84), R96 (≠ H116), A97 (≠ L117), F116 (≠ Y136), H128 (≠ F148), E158 (= E185)
binding zinc ion: E31 (≠ D48), H216 (= H252), H218 (= H254)

3mi3A Homocitrate synthase lys4 bound to lysine (see paper)
23% identity, 70% coverage: 42:440/572 of query aligns to 15:367/370 of 3mi3A

query
sites
3mi3A

M

I

S

E

T

S

D

T

L

L

R
|
R

D
x
E

G

G

N

E

Q
|
Q

A

F

L

A

F

N

E

A

P

F

M

F

N

D

S

T

E

E

R

K

K

K

M

I

R

Q

M

I

F

A

K

K

M

A

L

L

V

D

E

N

I

F

G

G

L

V

K

D

E

Y

I

I

E

E

V

L

A

T

F

S

P

P

A

V

A

A

S

S

Q

E

T

Q

D

S

F

R

D

Q

F

D

V

C

R

E

E

A

L

I

I

C

E

K

G

L

G

G

H

-

I

-

P

-

D

-

V

L

T

K

I

C

E

K

V

I

L

L

T

T

Q

H

A

I

R

R

P

C

H

H

L

M

I

D

E

D

R

A

T

-

F

-

E

-

S

-

V

-

R

-

G

-

A

-

K

-

R

-

A

-

I

-

V

-

H

-

V

-

Y

-

N

-

A

-

V

-

A

-

P

-

N

-

F

-

R

-

R

R

I

V

V

A

F

V

D

E

T

T

D

G

R

V

A

D

G

G

V

V

K
x
D

Q

V

I

V

A

I

V

G

D

T

S

S

A

M

R

T

Y

Y

F

I

V

I

E

D

M

S

A

A

A

T

R

E

Q

V

P

-

G

-

T

I

D

N

F

F

T

V

F

K

Q

S

Y

K

S

G

P

I

E

E

V

V

F

R

S

F

G

S

T

S

E

E

L

D

D

S

F

F

A

R

V

S

E

D

V

L

A

V

N

D

A

-

V

L

I

L

E

S

V

L

W

Y

Q

K

P

A

T

V

P

D

Q

K

Q

I

K

G

C

V

I

N

-

R

I

V

N

G

L

I

P

A

A

D

T
|
T

V

V

E

G

M

C

S

A

T

T

P

P

N

R

V

Q

Y

V

A

Y

D

D

Q

L

I

I

E

-

W

-

M

-

H

-

R

R

H

T

L

L

A

R

R

G

R

V

D

V

S

S

V

C

L

D

L

I

S

E

V

C

H
|
H

P

F

H
|
H

N

N

D

D

R

T

G

G

T

M

A

A

V

I

A

A

A

N

A

A

E

Y

L

C

A

A

V

L

M

E

A

A

G

G

A

A

D

T

R

H

V

I

E

D

G

T

C

S

L

I

F

L

G

G

N

I

G

G

E

E

R

R

T

N

G

G

N

I

V

T

D

P

L

L

V

G

T

A

L

L

A

L

L

A

N

R

L

M

Y

Y

-

V

-

T

-

D

S

R

Q

E

G

Y

V

I

H

T

P

H

G

K

L

Y

D

K

F

L

S

N

R

Q

I

L

N

R

E

E

V

L

A

E

R

N

T

L

V

V

E

A

H

D

C

A

T

V

Q

E

L

V

P

Q

I

I

H

-

P

P

R

F

H

N

P

N

Y

Y

V

I

-

T

G

G

D

M

L

C

V

A

F

F

T

T

A

H

F

K

S

A

G

G

S

I

H

H

Q

A

D

K

A

A

I

I

K

-

G

-

F

-

A

-

V

L

Q

A

Q

N

P

P

D

-

A

S

V

T

W

Y

E

E

V

I

P

-

Y

-

L

-

P

-

V

L

D

K

P

P

A

E

D

D

L

F

G

G

R

M

S

S

Y

-

E

-

S

R

I

Y

I

V

R

H

V

V

N

G

S

S

Q

R

-

L

S

T

G

G

K

W

G

N

G

A

I

I

A

K

Y

S

L

R

L

A

E

E

A

-

E

Q

Y

L

G

N

L

L

V

H

M

L

P

T

R

D

R

A

L

Q

Q

A

V

K

E

E

F

L

S

T

A

V

A

R

I

I

Q

K

R

K

I

L

T

A

D

D

E

V

R

R

G

-

T

-

E

T

L

L

S

A

A

M

G

D

D

D

I

V

W

D

R

R

A

V

F

L

E

R

E

E

active site: Q24 (= Q51)
binding lysine: R20 (= R47), D100 (≠ K156), T163 (= T221), H190 (= H252), H192 (= H254)
binding zinc ion: E21 (≠ D48), H190 (= H252), H192 (= H254)

Q9Y823 Homocitrate synthase, mitochondrial; HCS; EC 2.3.3.14 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see 2 papers)
24% identity, 70% coverage: 42:440/572 of query aligns to 38:401/418 of Q9Y823

query
sites
Q9Y823

M

I

S

E

T

S

D

T

L

L

R
|
R

D
x
E

G

G

N

E

Q
|
Q

A

F

L

A

F

N

E

A

P

F

M

F

N

D

S

T

E

E

R

K

K

K

M

I

R

Q

M

I

F

A

K

K

M

A

L

L

V

D

E

N

I

F

G

G

L

V

K

D

E

Y

I

I

E
|
E

V

L

A

T

F

S

P

P

A

V

A

A

S

S

Q

E

T

Q

D

S

F

R

D

Q

F

D

V

C

R

E

E

A

L

I

I

C

E

K

G

L

G

G

H

-

I

-

P

-

D

-

V

L

T

K

I

C

E

K

V

I

L

L

T

T

Q
x
H

A

I

R

R

P

C

H

H

L

M

I

D

E

D

R

A

T

R

F

V

E

A

S

V

V

E

R

T

G

G

A

V

K

D

R

G

A

-

I

-

V

V

H
x
D

V

V

Y

V

N

I

A

G

V

T

A

S

P

Q

N

Y

F

L

R

R

R

K

I

Y

V

S

F

H

D

G

T

K

D

D

R

M

A

T

G

Y

V

I

K

-

Q

-

I

-

A

-

V

I

D

D

S

S

A

A

R

T

Y

E

F

V

V

I

E

N

M

F

A

V

A

-

R

K

Q

S

P

K

G

G

T

I

D

E

F

V

T
x
R

F

F

Q
x
S

Y

-

S

S

P
x
E

E

D

V

S

F

F

S

R

G

S

T

D

E

L

L

V

D

D

F

L

A

-

V

L

E

S

V

L

A

Y

N

K

A

A

V

V

I

D

E

K

V

I

W

G

Q

V

P

N

T

R

P

-

Q

-

K

-

C

-

I

-

I

V

N

G

L

I

P

A

A

D

T
|
T

V

V

E

G

M

C

S

A

T

T

P

P

N

R

V

Q

Y

V

A

Y

D

D

Q

L

I

I

E

-

W

-

M

-

H

-

R

R

H

T

L

L

A

R

R

G

R

V

D

V

S

S

V

C

L

D

L

I

S
x
E

V

C

H
|
H

P

F

H
|
H

N

N

D

D

R

T

G

G

T

M

A

A

V

I

A

A

A

N

A

A

E

Y

L

C

A

A

V

L

M

E

A

A

G

G

A

A

D

T

R

H

V

I

E

D

G

T

C

S

L

I

F

L

G

G

N

I

G

G

E

E

R

R

T

N

G

G

N

I

V

T

D

P

L

L

V

G

T

A

L

L

A

L

L

A

N

R

L

M

Y

Y

-

V

-

T

-

D

S

R

Q

E

G

Y

V

I

H

T

P

H

G

K

L

Y

D

K

F

L

S

N

R

Q

I

L

N
x
R

E

E

V

L

A

E

R

N

T

L

V

V

E

A

H

D

C

A

T

V

Q

E

L

V

P

Q

I

I

H

-

P

P

R

F

H

N

P

N

Y

Y

V

I

-

T

G

G

D

M

L

C

V

A

F

F

T

T

A

H

F

K

S

A

G

G

S

I

H

H

Q

A

D

K

A

A

I

I

K

-

G

-

F

-

A

-

V

L

Q

A

Q

N

P

P

D

-

A

S

V

T

W
x
Y

E

E

V

I

P

-

Y

-

L

-

P

-

V

L

D

K

P

P

A

E

D

D

L

F

G

G

R

M

S

S

Y

-

E

-

S

R

I

Y

I

V

R

H

V

V

N

G

S

S

Q

R

-

L

S

T

G

G

K

W

G

N

G

A

I

I

A

K

Y

S

L

R

L

A

E

E

A

-

E
x
Q

Y

L

G

N

L

L

V

H

M

L

P

T

R

D

R

A

L

Q

Q

A

V

K

E

E

F

L

S

T

A

V

A

R

I

I

Q

K

R

K

I

L

T

A

D

D

E

V

R

R

G

-

T

-

E

T

L

L

S

A

A

M

G

D

D

D

I

V

W

D

R

R

A

V

F

L

E

R

E

E

R43 (= R47) binding 2-oxoglutarate; mutation R->A,K,Q: Abolishes the catalytic activity.
E44 (≠ D48) binding 2-oxoglutarate; binding L-lysine; binding Zn(2+)
Q47 (= Q51) mutation to A: Abolishes the catalytic activity.
E74 (= E78) mutation to A: Abolishes the catalytic activity.; mutation to Q: Results in a moderate decrease in the turnover number and a slight increase in the Km value for each substrate.
H103 (≠ Q112) binding 2-oxoglutarate; mutation to A: Substantially impairs catalytic efficiency.
D123 (≠ H134) binding L-lysine; mutation to N: Does not affect the catalytic activity but impairs L-lysine inhibition.
R163 (≠ T179) binding 2-oxoglutarate; mutation R->A,Q: Abolishes the catalytic activity.; mutation to K: Severely diminishes affinity for 2-oxoglutarate and substantially impairs catalytic efficiency.
S165 (≠ Q181) binding 2-oxoglutarate; mutation to A: Results in a moderate decrease in catalytic efficiency.
E167 (≠ P184) mutation E->A,Q: Abolishes the catalytic activity.
T197 (= T221) binding 2-oxoglutarate; binding L-lysine; mutation to A: Exhibits a 25-fold decrease in catalytic efficiency.; mutation to S: Results in a modest decrease in catalytic efficiency.; mutation to V: Abolishes the catalytic activity.
E222 (≠ S250) mutation to Q: Does not affect the catalytic activity but impairs L-lysine inhibition.
H224 (= H252) binding 2-oxoglutarate; binding Zn(2+)
H226 (= H254) binding 2-oxoglutarate; binding Zn(2+)
R288 (≠ N313) mutation to K: Does not affect the catalytic activity but impairs L-lysine inhibition.
Y332 (≠ W363) mutation to A: Abolishes the catalytic activity.; mutation to F: Results in a decrease in catalytic efficiency.
Q364 (≠ E401) mutation to R: Does not affect the catalytic activity but impairs L-lysine inhibition.

3ivtB Homocitrate synthase lys4 bound to 2-og (see paper)
24% identity, 70% coverage: 42:440/572 of query aligns to 33:396/400 of 3ivtB

query
sites
3ivtB

M

I

S

E

T

S

D

T

L

L

R
|
R

D
x
E

G

G

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E

Q
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Q

A

F

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A

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N

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A

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M

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K

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x
H

A

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C

H

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R

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F

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G

A

-

I

-

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Q

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K

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x
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x
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T

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C

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H
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H

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H

N

N

D

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A

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A

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A

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A

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Y

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L

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-

V

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A

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D

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G

R

M

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S

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-

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-

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R

I

Y

I

V

R

H

V

V

N

G

S

S

Q

R

-

L

S

T

G

G

K

W

G

N

G

A

I

I

A

K

Y

S

L

R

L

A

E

E

A

-

E

Q

Y

L

G

N

L

L

V

H

M

L

P

T

R

D

R

A

L

Q

Q

A

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K

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F

L

S

T

A

V

A

R

I

I

Q

K

R

K

I

L

T

A

D

D

E

V

R

R

G

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T

-

E

T

L

L

S

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A

M

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V

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R

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L

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R

E

E

active site: Q42 (= Q51)
binding 2-oxoglutaric acid: R38 (= R47), H98 (≠ Q112), R158 (≠ T179), S160 (≠ Q181), T192 (= T221), H219 (= H252), H221 (= H254)
binding zinc ion: E39 (≠ D48), H219 (= H252), H221 (= H254)

Q8F3Q1 (R)-citramalate synthase CimA; LiCMS; EC 2.3.3.21 from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) (see 2 papers)
28% identity, 25% coverage: 216:359/572 of query aligns to 174:315/516 of Q8F3Q1

query
sites
Q8F3Q1

I

I

N

F

L

L

P

P

A

D

T
|
T

V

L

E

G

M

V

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L

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V

-

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-

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H

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H

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A

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A

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A

A

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G

A

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N

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H

Q

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|
D

A

G

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D

K

K

G

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F
x
N

A
x
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x
Y

Q

A

Q

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P

T179 (= T221) binding pyruvate; mutation to A: 16.4-fold increase in Km for pyruvate and 186-fold decrease in kcat.
H302 (= H346) mutation H->A,N: Loss of activity.
D304 (= D348) mutation to A: 5.2-fold increase in Km for acetyl-CoA and 16.6-fold decrease in kcat.
N310 (≠ F354) mutation to A: 2.2-fold increase in Km for acetyl-CoA and 1.7-fold decrease in kcat.
L311 (≠ A355) mutation to A: 8-fold increase in Km for acetyl-CoA and 6-fold decrease in kcat.
Y312 (≠ V356) mutation to A: Loss of activity.

Sites not aligning to the query:

16 mutation R->K,Q: Loss of activity.
16:17 binding pyruvate
17 D→A: 34-fold increase in Km for pyruvate and 315-fold decrease in kcat.; D→N: 4.4-fold increase in Km for pyruvate and 480-fold decrease in kcat.
81 L→A: 4.7-fold increase in Km for pyruvate and 15.7-fold decrease in kcat.; L→V: 3.3-fold increase in Km for pyruvate and 10.1-fold decrease in kcat.
83 F→A: 5-fold increase in Km for acetyl-CoA and 120-fold decrease in kcat.
104 L→V: 1.8-fold increase in Km for pyruvate and 3.4-fold decrease in kcat.
144 binding pyruvate; Y→L: 259-fold increase in Km for pyruvate and 76-fold decrease in kcat.; Y→V: 114-fold increase in Km for pyruvate and 5.3-fold decrease in kcat.
146 mutation E->D,Q: Minor effects on the binding of acetyl-CoA, but causes a strong decrease in kcat.
430 Y→L: No change in Km for acetyl-CoA and 2.3-fold decrease in kcat. Severely impairs inhibition by isoleucine.
431 D→A: 1.8-fold decrease in Km for acetyl-CoA and 5-fold decrease in kcat.
451 L→V: 1.5-fold increase in Km for acetyl-CoA and 4.3 decrease in kcat.
454 Y→A: 1.4 decrease in Km for acetyl-CoA and 17-fold decrease in kcat. Still inhibited by isoleucine and weakly inhibited by leucine.
458 I→A: 1.3-fold decrease in Km for acetyl-CoA and 14-fold decrease in kcat. Abolishes inhibition by isoleucine.
464 T→A: 1.8-fold decrease in Km for acetyl-CoA and 4.3-fold decrease in kcat.
468 V→A: No change in Km for acetyl-CoA and 2-fold decrease in kcat. Increases inhibition by isoleucine and leucine becomes an effective inhibitor.
493 P→A: 1.5-fold decrease in Km for acetyl-CoA and 2.6-fold decrease in kcat.
495 Q→A: 1.6-fold decrease in Km for acetyl-CoA and 2.8-fold decrease in kcat.

3ivsA Homocitrate synthase lys4 (see paper)
23% identity, 59% coverage: 42:378/572 of query aligns to 15:307/364 of 3ivsA

query
sites
3ivsA

M

I

S

E

T

S

D

T

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L

R

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x
E

G

G

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H

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F

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N

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Y

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A

F

F

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T

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F

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S

A

G

G

S

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H

H

Q

A

D

K

A

A

I

I

K

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G

-

F

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A

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V

L

Q

A

Q

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P

P

D

-

A

S

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Y

E

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Y

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L

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G

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M

S

S

active site: Q24 (= Q51)
binding cobalt (ii) ion: E21 (≠ D48), H188 (= H252), H190 (= H254)

Q53WI0 4-hydroxy-2-oxovalerate aldolase; HOA; 4-hydroxy-2-keto-pentanoic acid aldolase; 4-hydroxy-2-oxohexanoate aldolase; 4-hydroxy-2-oxopentanoate aldolase; EC 4.1.3.39; EC 4.1.3.43 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
25% identity, 53% coverage: 29:333/572 of query aligns to 2:278/347 of Q53WI0

query
sites
Q53WI0

T

S

W

W

P

-

D

D

N

L

T

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I

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A

A

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K

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A

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H

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F

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K

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A

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M

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-

V

-

F

-

S

-

G

G

T

M

E

A

L

K

D

E

F

M

A

G

V

L

E

E

V

A

A

V

N

G

A

F

V

L

I

M

E

M

V

S

W

H

Q

M

P

R

T

P

P

P

-

E

-

F

-

L

-

A

-

E

Q

Q

Q

A

K

R

C

L

I

M

I

E

N

G

L

Y

P

G

A

A

T

D

V

V

-

V

-

Y

-

I

-

V

-

D

-

S

-

A

-

G

E

A

M

M

S

L

T

P

P

E

N

D

V

A

Y

Y

A

A

D

-

Q

R

I

V

E

K

W

A

M

L

H

K

R

E

H

A

L

L

A

S

R

R

R

A

D

K

S

-

V

-

L

-

L

V

S

G

V

F

H

H

P

A

H

H

N

N

D

N

R

L

G

G

T

L

A

A

V

I

A

G

A

N

A

T

E

L

L

A

A

A

V

L

M

A

A

A

G

G

A

A

D

D

R

W

V

V

E

D

G

A

C

T

L

L

F

R

G

G

N

Y

G

G

E

A

R

G

T

A

G

G

N

N

V

A

D

P

L

L

V

E

T

V

L

L

A

A

L

A

N

V

L

L

Y

D

S

K

Q

A

G

G

V

L

H

N

P

P

G

G

L

L

D

D

F

V

S

F

R

K

I

L

N

L

E

D

V

A

A

A

R

E

T

Y

V

V

E

M

H

G

C

-

T

-

Q

-

L

-

P

P

I

I

H

L

P

H

R

F

H

Q

P

P

Y

Y

Sites not aligning to the query:

324 A→G: Increases the channeling efficiency of propanaldehyde from 57% to 94%.

Query Sequence

>WP_011767245.1 NCBI__GCF_000061505.1:WP_011767245.1
MLKNPHTKYRPANPFAGVVPGGRGLSDRTWPDNTITRPPVWMSTDLRDGNQALFEPMNSE
RKMRMFKMLVEIGLKEIEVAFPAASQTDFDFVRELIEGGHIPDDVTIEVLTQARPHLIER
TFESVRGAKRAIVHVYNAVAPNFRRIVFDTDRAGVKQIAVDSARYFVEMAARQPGTDFTF
QYSPEVFSGTELDFAVEVANAVIEVWQPTPQQKCIINLPATVEMSTPNVYADQIEWMHRH
LARRDSVLLSVHPHNDRGTAVAAAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYS
QGVHPGLDFSRINEVARTVEHCTQLPIHPRHPYVGDLVFTAFSGSHQDAIKKGFAVQQPD
AVWEVPYLPVDPADLGRSYESIIRVNSQSGKGGIAYLLEAEYGLVMPRRLQVEFSAAIQR
ITDERGTELSAGDIWRAFEEEYLAVAAPYAYVEHHLAEHGGQQGISLTVEEAGVRRVLRG
VGNGPIDAALHALDAGAVLLGYEERAIGQGGDARAVAYIELADGEGGGSTFGVGIHANIV
TASVLAIVSALDRLAQRRERADGVGRQVAATR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory