SitesBLAST
Comparing WP_011778289.1 NCBI__GCF_000015305.1:WP_011778289.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
2p9eA Crystal structure of g336v mutant of e.Coli phosphoglycerate dehydrogenase (see paper)
30% identity, 88% coverage: 13:293/321 of query aligns to 16:295/406 of 2p9eA
- active site: N104 (≠ H91), R236 (= R232), D260 (= D256), E265 (≠ Y261), H288 (= H286)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G156 (= G148), H157 (= H149), I158 (= I150), Y176 (≠ V168), D177 (≠ T169), I178 (≠ V174), H206 (≠ S202), V207 (≠ A203), P208 (= P204), S212 (≠ H208), A234 (≠ V230), S235 (≠ G231), R236 (= R232), H288 (= H286), G290 (≠ S288)
2gsdA NAD-dependent formate dehydrogenase from bacterium moraxella sp.C2 in complex with NAD and azide (see paper)
32% identity, 80% coverage: 36:291/321 of query aligns to 84:337/399 of 2gsdA
- active site: N146 (≠ H91), R284 (= R232), D308 (= D256), Q313 (≠ Y261), H332 (= H286)
- binding azide ion: P97 (≠ R49), F98 (= F50), I122 (≠ A69), R284 (= R232), H332 (= H286)
- binding nicotinamide-adenine-dinucleotide: I122 (≠ A69), N146 (≠ H91), V150 (≠ I95), A198 (≠ G146), G200 (= G148), R201 (≠ H149), I202 (= I150), D221 (≠ G170), R222 (≠ S171), P256 (= P204), H258 (≠ N206), T261 (= T209), T282 (≠ V230), A283 (≠ G231), R284 (= R232), D308 (= D256), H332 (= H286), S334 (= S288), G335 (= G289)
Sites not aligning to the query:
1ybaA The active form of phosphoglycerate dehydrogenase (see paper)
29% identity, 88% coverage: 13:293/321 of query aligns to 16:295/406 of 1ybaA
- active site: N104 (≠ H91), R236 (= R232), D260 (= D256), E265 (≠ Y261), H288 (= H286)
- binding 2-oxoglutaric acid: R56 (= R49), S57 (vs. gap), C79 (≠ A68), I80 (≠ A69)
- binding nicotinamide-adenine-dinucleotide: I80 (≠ A69), F102 (= F89), V108 (≠ I95), G154 (= G146), G156 (= G148), H157 (= H149), I158 (= I150), Y176 (≠ V168), D177 (≠ T169), I178 (≠ V174), K181 (≠ D177), H206 (≠ S202), V207 (≠ A203), P208 (= P204), A234 (≠ V230), S235 (≠ G231), R236 (= R232), H288 (= H286), G290 (≠ S288)
- binding phosphate ion: G81 (= G70), N83 (≠ D72)
1psdA The allosteric ligand site in the vmax-type cooperative enzyme phosphoglycerate dehydrogenase (see paper)
29% identity, 88% coverage: 13:293/321 of query aligns to 14:293/404 of 1psdA
- active site: N102 (≠ H91), R234 (= R232), D258 (= D256), E263 (≠ Y261), H286 (= H286)
- binding nicotinamide-adenine-dinucleotide: N102 (≠ H91), H155 (= H149), I156 (= I150), D175 (≠ T169), I176 (≠ V174), K179 (≠ D177), H204 (≠ S202), V205 (≠ A203), P206 (= P204), A232 (≠ V230), S233 (≠ G231), R234 (= R232), H286 (= H286)
Sites not aligning to the query:
P0A9T0 D-3-phosphoglycerate dehydrogenase; PGDH; 2-oxoglutarate reductase; EC 1.1.1.95; EC 1.1.1.399 from Escherichia coli (strain K12) (see 2 papers)
29% identity, 88% coverage: 13:293/321 of query aligns to 20:299/410 of P0A9T0
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
2nadA High resolution structures of holo and apo formate dehydrogenase (see paper)
33% identity, 80% coverage: 34:291/321 of query aligns to 82:337/391 of 2nadA
- active site: N146 (≠ H91), R284 (= R232), D308 (= D256), Q313 (≠ Y261), H332 (= H286)
- binding azide ion: P97 (≠ R49), F98 (= F50), I122 (≠ A69), N146 (≠ H91), R284 (= R232)
- binding nicotinamide-adenine-dinucleotide: I122 (≠ A69), N146 (≠ H91), V150 (≠ I95), A198 (≠ G146), G200 (= G148), R201 (≠ H149), I202 (= I150), D221 (≠ G170), P256 (= P204), E260 (≠ H208), T261 (= T209), T282 (≠ V230), A283 (≠ G231), R284 (= R232), D308 (= D256), H332 (= H286), S334 (= S288), G335 (= G289)
Sites not aligning to the query:
P33160 Formate dehydrogenase; FDH; NAD-dependent formate dehydrogenase; EC 1.17.1.9 from Pseudomonas sp. (strain 101) (Achromobacter parvulus T1) (see 3 papers)
33% identity, 80% coverage: 34:291/321 of query aligns to 83:338/401 of P33160
- I123 (≠ A69) binding substrate
- N147 (≠ H91) binding substrate
- S148 (≠ E92) binding NAD(+)
- RI 202:203 (≠ HI 149:150) binding NAD(+)
- D222 (≠ G170) binding NAD(+)
- C256 (≠ A203) mutation C->S,M: High resistance to inactivation by Hg(2+), Increased stability at 25 degree Celsius and decreased thermostability at 45 degree Celsius.
- PLHPE 257:261 (≠ PLNRH 204:208) binding NAD(+)
- T283 (≠ V230) binding NAD(+)
- D309 (= D256) binding NAD(+)
- HISG 333:336 (≠ HTSG 286:289) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 381 binding NAD(+)
6jujA Crystal structure of formate dehydrogenase mutant v198i/c256i/p260s/e261p/s381n/s383f from pseudomonas sp. 101in complex with non-natural cofactor nicotinamide cytosine dinucleotide (see paper)
33% identity, 80% coverage: 34:291/321 of query aligns to 82:337/381 of 6jujA
- binding [[(2S,3S,4R,5S)-5-(3-aminocarbonylpyridin-1-ium-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2S,3S,4R,5S)-5-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl hydrogen phosphate: F98 (= F50), N146 (≠ H91), V150 (≠ I95), G200 (= G148), I202 (= I150), D221 (≠ G170), R222 (≠ S171), I255 (≠ A203), P256 (= P204), H258 (≠ N206), T282 (≠ V230), A283 (≠ G231), D308 (= D256), H332 (= H286), S334 (= S288), G335 (= G289)
8oq2A Binding of NADP to a formate dehydrogenase from starkeya novella.
32% identity, 80% coverage: 34:291/321 of query aligns to 83:338/381 of 8oq2A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: I123 (≠ A69), N147 (≠ H91), V151 (≠ I95), G201 (= G148), R202 (≠ H149), I203 (= I150), Q222 (≠ G170), R223 (≠ S171), H224 (≠ G172), V256 (≠ A203), P257 (= P204), H259 (≠ N206), T262 (= T209), T283 (≠ V230), A284 (≠ G231), R285 (= R232), D309 (= D256), H333 (= H286), S335 (= S288), G336 (= G289)
Sites not aligning to the query:
5mhaB D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with a mixture of 2-ketohexanoic acid and 2-hydroxyhexanoic acid, and NADPH (1.57 a resolution)
30% identity, 80% coverage: 64:319/321 of query aligns to 62:307/308 of 5mhaB
- binding 2-Ketohexanoic acid: R66 (≠ A68), R226 (= R232), H274 (= H286), Y282 (≠ T294)
- binding (2R)-2-hydroxyhexanoic acid: R66 (≠ A68), A67 (= A69), G68 (= G70), H91 (= H91), Y282 (≠ T294)
- binding magnesium ion: F212 (≠ L218), E213 (≠ K219), M215 (≠ L221), D243 (≠ A249)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A67 (= A69), G68 (= G70), T88 (= T88), L143 (≠ F147), G144 (= G148), T145 (≠ H149), L146 (≠ I150), R165 (≠ T169), R166 (≠ G170), S167 (= S171), P180 (≠ D184), T197 (≠ A203), P198 (= P204), T203 (= T209), V224 (= V230), A225 (≠ G231), R226 (= R232), H274 (= H286), S276 (= S288)
5mhaA D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with a mixture of 2-ketohexanoic acid and 2-hydroxyhexanoic acid, and NADPH (1.57 a resolution)
30% identity, 80% coverage: 64:319/321 of query aligns to 62:307/308 of 5mhaA
- binding 2-Ketohexanoic acid: R66 (≠ A68), A67 (= A69), G68 (= G70), H91 (= H91), R226 (= R232), H274 (= H286), Y282 (≠ T294)
- binding magnesium ion: T132 (= T136), A134 (= A138)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A67 (= A69), G68 (= G70), T88 (= T88), L143 (≠ F147), G144 (= G148), T145 (≠ H149), L146 (≠ I150), R165 (≠ T169), R166 (≠ G170), S167 (= S171), P180 (≠ D184), T197 (≠ A203), P198 (= P204), T203 (= T209), V224 (= V230), A225 (≠ G231), R226 (= R232), H274 (= H286), S276 (= S288)
5mh5A D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with 2-keto-hexanoic acid and NADP+ (1.4 a resolution)
30% identity, 80% coverage: 64:319/321 of query aligns to 62:307/308 of 5mh5A
- binding 2-Ketohexanoic acid: R66 (≠ A68), A67 (= A69), G68 (= G70), H91 (= H91), R226 (= R232), H274 (= H286), Y282 (≠ T294)
- binding magnesium ion: T132 (= T136), A134 (= A138), F212 (≠ L218), E213 (≠ K219), M215 (≠ L221), D243 (≠ A249)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: A67 (= A69), G68 (= G70), T88 (= T88), G142 (= G146), L143 (≠ F147), G144 (= G148), T145 (≠ H149), L146 (≠ I150), R165 (≠ T169), R166 (≠ G170), S167 (= S171), T197 (≠ A203), P198 (= P204), T203 (= T209), V224 (= V230), A225 (≠ G231), R226 (= R232), H274 (= H286), S276 (= S288)
5mh6A D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with 2-ketohexanoic acid and NAD+ (1.35 a resolution)
30% identity, 80% coverage: 64:319/321 of query aligns to 60:305/306 of 5mh6A
- binding 2-Ketohexanoic acid: R64 (≠ A68), A65 (= A69), G66 (= G70), H89 (= H91), R224 (= R232), H272 (= H286), Y280 (≠ T294)
- binding magnesium ion: T130 (= T136), A132 (= A138), F210 (≠ L218), E211 (≠ K219), M213 (≠ L221), G225 (= G233), P226 (= P234), V228 (= V236), E230 (= E238), D241 (≠ A249), S251 (≠ Y259)
- binding nicotinamide-adenine-dinucleotide: A65 (= A69), G66 (= G70), T86 (= T88), H89 (= H91), G142 (= G148), T143 (≠ H149), L144 (≠ I150), R164 (≠ G170), P196 (= P204), T201 (= T209), V222 (= V230), A223 (≠ G231), R224 (= R232), H272 (= H286), S274 (= S288)
3wr5A Structural basis on the efficient co2 reduction of acidophilic formate dehydrogenase
32% identity, 80% coverage: 34:291/321 of query aligns to 87:342/401 of 3wr5A
- active site: N151 (≠ H91), R289 (= R232), D313 (= D256), Q318 (≠ Y261), H337 (= H286)
- binding nicotinamide-adenine-dinucleotide: N151 (≠ H91), V155 (≠ I95), A203 (≠ G146), G205 (= G148), R206 (≠ H149), I207 (= I150), D226 (≠ G170), R227 (≠ S171), V260 (≠ A203), P261 (= P204), T287 (≠ V230), A288 (≠ G231), R289 (= R232), D313 (= D256), H337 (= H286), S339 (= S288), G340 (= G289)
Sites not aligning to the query:
6ttbA Crystal structure of NAD-dependent formate dehydrogenase from staphylococcus aureus in complex with NAD
29% identity, 85% coverage: 45:317/321 of query aligns to 63:323/331 of 6ttbA
- binding nicotinamide-adenine-dinucleotide: V87 (≠ A69), N111 (= N87), V115 (≠ I95), F162 (≠ V145), G165 (= G148), R166 (≠ H149), I167 (= I150), Y185 (≠ V168), D186 (≠ T169), P187 (≠ G170), H214 (≠ S202), A215 (= A203), P216 (= P204), T221 (= T209), T242 (≠ V230), A243 (≠ G231), R244 (= R232), H292 (= H286), S294 (= S288), G295 (= G289)
3n7uA NAD-dependent formate dehydrogenase from higher-plant arabidopsis thaliana in complex with NAD and azide
30% identity, 71% coverage: 67:293/321 of query aligns to 93:312/351 of 3n7uA
- active site: N119 (= N87), R257 (= R232), D281 (= D256), Q286 (≠ Y261), H305 (= H286)
- binding azide ion: I95 (≠ A69), R257 (= R232), H305 (= H286)
- binding nicotinamide-adenine-dinucleotide: I95 (≠ A69), N119 (= N87), V123 (≠ I95), G173 (= G148), R174 (≠ H149), I175 (= I150), D194 (vs. gap), R195 (vs. gap), M228 (≠ A203), P229 (= P204), N255 (≠ V230), A256 (≠ G231), R257 (= R232), D281 (= D256), H305 (= H286), S307 (= S288), G308 (= G289)
Sites not aligning to the query:
3jtmA Structure of recombinant formate dehydrogenase from arabidopsis thaliana
30% identity, 71% coverage: 67:293/321 of query aligns to 93:312/351 of 3jtmA
Q9S7E4 Formate dehydrogenase, chloroplastic/mitochondrial; FDH; NAD-dependent formate dehydrogenase; EC 1.17.1.9 from Arabidopsis thaliana (Mouse-ear cress)
30% identity, 71% coverage: 67:293/321 of query aligns to 126:345/384 of Q9S7E4
- RI 207:208 (≠ HI 149:150) binding NAD(+)
- D227 (vs. gap) binding NAD(+)
- PLTEK 262:266 (≠ PLNRH 204:208) binding NAD(+)
- N288 (≠ V230) binding NAD(+)
- D314 (= D256) binding NAD(+)
- HTSG 338:341 (= HTSG 286:289) binding NAD(+)
Sites not aligning to the query:
- 1:29 modified: transit peptide, Chloroplast and mitochondrion
7arzA Ternary complex of NAD-dependent formate dehydrogenase from physcomitrium patens
30% identity, 81% coverage: 36:296/321 of query aligns to 66:325/361 of 7arzA
- binding azide ion: T77 (≠ G47), P79 (≠ R49), F80 (= F50), L99 (= L64), V100 (≠ H65), T101 (≠ P66), G103 (≠ A68), V104 (≠ A69), R267 (= R232), H315 (= H286)
- binding nicotinamide-adenine-dinucleotide: V104 (≠ A69), N128 (≠ H91), V132 (≠ I95), G182 (= G148), R183 (≠ H149), I184 (= I150), D204 (≠ G170), R205 (≠ S171), T238 (≠ A203), P239 (= P204), Q243 (≠ H208), N265 (≠ V230), A266 (≠ G231), R267 (= R232), D291 (= D256), H315 (= H286), S317 (= S288), G318 (= G289)
Sites not aligning to the query:
3bazA Structure of hydroxyphenylpyruvate reductase from coleus blumei in complex with NADP+ (see paper)
29% identity, 82% coverage: 52:315/321 of query aligns to 57:306/311 of 3bazA
- active site: L98 (≠ H91), R230 (= R232), A251 (= A253), D254 (= D256), E259 (≠ Y261), H277 (= H286)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V74 (≠ A69), G149 (= G146), L150 (≠ F147), G151 (= G148), R152 (≠ H149), I153 (= I150), S172 (≠ T169), R173 (≠ G170), S174 (= S171), C201 (≠ A203), P202 (= P204), T207 (= T209), I228 (≠ V230), G229 (= G231), R230 (= R232), D254 (= D256), H277 (= H286), G279 (≠ S288)
Query Sequence
>WP_011778289.1 NCBI__GCF_000015305.1:WP_011778289.1
MRVLVVDPNLVPHRERLEAAMPPGTEVLWHVAGVPEDELRKAEVFVGSRFTAEMARLAEN
LRLLHPAAAGTDKIDLSALSADVLVANTFHHERSIAEYVVGAAVMLRRDFLTQDARLRDG
VWASSVYDSTVPQPSTLADAHVGFVGFGHIGRHTWKLLQVFGCTAAAVTGSGRVADDAGL
AWVDDTGKLERLMRECDVVVVSAPLNRHTEGMIGAAELKALGPDGVLINVGRGPLVQERA
LYDALAGGAIKAAAVDVWYRYPADGTGCAPSDLPFATLPGILMTPHTSGVTTDTFKGRVD
DVAANVGRLQNGEPLRNLVPH
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory