SitesBLAST

1e19A Structure of the carbamate kinase-like carbamoyl phosphate synthetase from the hyperthermophilic archaeon pyrococcus furiosus bound to adp (see paper)
44% identity, 95% coverage: 2:299/314 of query aligns to 3:311/313 of 1e19A

query
sites
1e19A

R

R

I

V

V

V

I

I

A

A

L

L

G

G

G
|
G

N
|
N

A

A

I

L

L

Q

R

Q

R

R

G

G

Q

Q

P

K

M

G

T

S

A

Y

D

E

N

E

Q

M

R

M

E

D

N

N

I

V

R

R

S

K

A

T

A

A

Q

R

S

Q

I

I

A

A

-

E

T

I

V

I

A

A

E

R

G

G

N

Y

Q

E

L

V

V

V

I

I

A

T

H

H

G

G

N

N

G

G

P

P

Q

Q

V

V

G

G

L

S

L

L

A

L

L

L

Q

H

-

M

-

D

-

A

A

G

A

Q

S

A

Y

T

H

Y

D

G

V

I

A

P

P

A

Y

Q

P

P

L

M

D

D

V

V

L

A

G

G

A

A

Q

M

T

S

E

Q

A

G

M

W

I

I

G

G

Y

Y

V

M

I

I

E

Q

Q

Q

E

A

L

L

G

K

N

N

V

E

L

L

P

R

P

K

-

R

-

G

-

M

E

E

Q

K

P

K

L

V

A

V

T

T

V

I

L

I

T

T

M

Q

I

T

E

I

V

V

A

D

P

K

A

N

D

D

P

P

A

A

F

F

A

Q

N

N

P

P

T

T

K
|
K

P

P

I

V

G

G

P

P

V

F

Y

Y

D

D

R

E

Q

E

T

T

A

A

D

K

A

R

M

L

H

A

A

R

A

E

S

K

G

G

W

W

T

I

F

V

A

K

P

E

D

D

-

S

G

G

E

R

H

G

F

W

R

R

V

V

I

V

A

P

S

S

P

P

K

D

P

P

K

K

R

G

I

H

F

V

E

E

I

A

G

E

P

T

I

I

R

K

T

K

L

L

V

V

E

E

H

R

G

G

T

V

M

I

V

V

I

I

C

A

A

S

G

G

I

V

P

P

T

V

V

I

Y

L

D

E

G

-

Q

D

G

G

R

E

L

I

Q

K

G

G

V

V

E

E

A

A

V

V

I

I

D

D

K
|
K

D

D

L

L

A

A

S

G

A

E

L

K

L

L

A

A

E

E

Q

E

L

V

H

N

A

A

D

D

M

I

L

F

V

M

I

I

A

L

T
|
T

D
|
D

V
|
V

D

N

G

G

V

A

Y
x
A

T

L

G

Y

W
x
Y

G

G

T

T

P

E

D

K

Q

E

R

Q

R

W

L

L

S

R

R

E

I

V

T

K

P

V

D

E

E

E

L

L

-

R

-

K

-

Y

E

E

T

E

M

G

H

H

F

F

A
x
K

A

A

G

G

S
|
S

M
|
M

G

G

P

P

K
|
K

V

V

E

L

A

A

C

I

R

R

F

F

V

I

R

E

N

W

T

G

G

G

R

E

P

R

A

A

S

I

I

I

G

A

A

H

L

L

G

E

D

K

I

A

A

V

R

E

I

A

A

L

A

E

G

G

Q

K

A

T

G

G

T

T

R

Q

V

V

active site: G10 (= G9), N11 (= N10), K130 (= K122), K214 (= K206), K276 (= K264)
binding adenosine-5'-diphosphate: G10 (= G9), T234 (= T226), D235 (= D227), V236 (= V228), A240 (≠ Y232), Y243 (≠ W235), K269 (≠ A257), S272 (= S260), M273 (= M261), K276 (= K264)

4jz8A Carbamate kinase from giardia lamblia bound to citric acid (see paper)
42% identity, 95% coverage: 3:301/314 of query aligns to 7:315/316 of 4jz8A

query
sites
4jz8A

I

V

V

V

I

I

A

A

L

L

G
|
G

N
|
N

A

A

I

M

L

L

R

Q

R

A

G

K

Q

E

P

K

M

G

T

D

A

Y

D

D

N

T

Q

Q

R

R

E

K

N

N

I

V

R

E

S

I

A

A

Q

S

S

E

I

I

A

Y

T

K

V

I

A

H

E

K

-

A

G

G

N

Y

Q

K

L

V

V

V

I

L

A

T

H

S

G
|
G

N

N

G
|
G

P
|
P

Q

Q

V

V

G

G

L

A

L

I

A

K

L

L

Q

Q

A

N

A

Q

S

A

Y

A

H

A

D

G

V

V

A

S

P

P

-

E

Y

M

P

P

L

L

D

H

V

V

L

C

G

G

A

A

Q

M

T

S

E

Q

A

G

M

F

I

I

G

G

Y

Y

V

M

I

M

E

S

Q

Q

E

A

L

M

G

D

N

N

V

V

L

F

P

C

P

A

E

N

Q

N

P

E

L

P

A

A

T

N

V

C

L

V

T

T

M

C

I

V

E

T

-

Q

-

T

-

L

V

V

A

D

P

P

A

K

D

D

P

Q

A

A

F

F

A

T

N

N

P

P

T

T

K
|
K

P

P

I

V

G

G

P

R

V

F

Y

Y

D

T

R

E

Q

Q

T

E

A

A

-

K

D

D

A

L

M

M

H

A

A

A

A

N

S

P

G

G

W

K

T

I

F

L

A

R

P

E

D

D

-

A

G

G

E

R

H

G

F

W

R

R

R

V

V

V

I

V

A

P

S

S

P

P

K

R

P

P

K

L

R

E

I

I

F

V

E

E

I

Y

G

G

P

V

I

I

R

K

T

T

L

L

V

I

E

D

H

N

G

N

T

V

M

L

V

V

I

I

C

C

A

T

G

N

G

G

I

I

P

P

T

C

V

K

Y

R

D

E

G

N

Q

K

G

-

R

V

L

I

Q

S

G

G

V

V

E

D

A

A

V

V

I

I

D

D

K
|
K

D

D

L

L

A

A

S

T

A

S

L

L

A

A

E

K

Q

T

L

L

H

N

A

S

D

D

M

Y

L

L

V

M

I

I

A

L

T

T

D

D

V

V

D

L

G

N

V

A

Y

C

T

I

G

N

W

Y

G

K

T

K

P

P

D

D

Q

E

R

R

R

K

L

L

S

E

R

E

I

I

T

K

P

L

D

S

E

E

L

I

E

L

T

A

M

L

-

E

-

K

-

D

-

G

H

H

F

F

A

A

G

G

S

S

M

M

G

G

P

P

K
|
K

V

V

E

R

A

A

C

I

R

E

F

F

V

T

R

Q

N

A

T

T

G

G

R

K

P

M

A

S

S

I

I

I

G

T

A

S

L

L

G

S

D

T

I

A

A

V

R

D

I

A

A

L

A

N

G

G

Q

K

A

C

G

G

T

T

R

R

V

I

A

I

Q

K

active site: G13 (= G9), N14 (= N10), K131 (= K122), K216 (= K206), K278 (= K264)
binding citric acid: G12 (= G8), G13 (= G9), G54 (= G49), G56 (= G51), P57 (= P52), K216 (= K206)

4jz7C Carbamate kinase from giardia lamblia bound to amp-pnp (see paper)
42% identity, 95% coverage: 3:301/314 of query aligns to 7:315/316 of 4jz7C

query
sites
4jz7C

I

V

V

V

I

I

A

A

L

L

G

G

G
|
G

N
|
N

A

A

I

M

L

L

R

Q

R

A

G

K

Q

E

P

K

M

G

T

D

A

Y

D

D

N

T

Q

Q

R

R

E

K

N

N

I

V

R

E

S

I

A

A

Q

S

S

E

I

I

A

Y

T

K

V

I

A

H

E

K

-

A

G

G

N

Y

Q

K

L

V

V

V

I

L

A

T

H

S

G

G

N

N

G

G

P

P

Q

Q

V

V

G

G

L

A

L

I

A

K

L

L

Q

Q

A

N

A

Q

S

A

Y

A

H

A

D

G

V

V

A

S

P

P

-

E

Y

M

P

P

L

L

D

H

V

V

L

C

G

G

A

A

Q

M

T

S

E

Q

A

G

M

F

I

I

G

G

Y

Y

V

M

I

M

E

S

Q

Q

E

A

L

M

G

D

N

N

V

V

L

F

P

C

P

A

E

N

Q

N

P

E

L

P

A

A

T

N

V

C

L

V

T

T

M

C

I

V

E

T

-

Q

-

T

-

L

V

V

A

D

P

P

A

K

D

D

P

Q

A

A

F

F

A

T

N

N

P

P

T

T

K
|
K

P

P

I

V

G

G

P

R

V

F

Y

Y

D

T

R

E

Q

Q

T

E

A

A

-

K

D

D

A

L

M

M

H

A

A

A

A

N

S

P

G

G

W

K

T

I

F

L

A

R

P

E

D

D

-

A

G

G

E

R

H

G

F

W

R

R

R

V

V

V

I

V

A

P

S

S

P

P

K

R

P

P

K

L

R

E

I

I

F

V

E

E

I

Y

G

G

P

V

I

I

R

K

T

T

L

L

V

I

E

D

H

N

G

N

T

V

M

L

V

V

I

I

C

C

A

T

G

N

G

G

I

I

P

P

T

C

V

K

Y

R

D

E

G

N

Q

K

G

-

R

V

L

I

Q

S

G

G

V

V

E

D

A

A

V

V

I

I

D

D

K
|
K

D

D

L

L

A

A

S

T

A

S

L

L

A

A

E

K

Q

T

L

L

H

N

A

S

D

D

M

Y

L

L

V

M

I

I

A

L

T
|
T

D
|
D

V

V

D

L

G

N

V

A

Y
x
C

T

I

G

N

W
x
Y

G

K

T

K

P

P

D

D

Q

E

R

R

R

K

L

L

S

E

R

E

I

I

T

K

P

L

D

S

E

E

L

I

E

L

T

A

M

L

-

E

-

K

-

D

-

G

H

H

F

F

A

A

G

G

S
|
S

M
|
M

G

G

P

P

K
|
K

V

V

E

R

A

A

C

I

R

E

F

F

V

T

R

Q

N

A

T

T

G

G

R

K

P

M

A

S

S

I

I

I

G

T

A

S

L

L

G

S

D

T

I

A

A

V

R

D

I

A

A

L

A

N

G

G

Q

K

A

C

G

G

T

T

R

R

V

I

A

I

Q

K

active site: G13 (= G9), N14 (= N10), K131 (= K122), K216 (= K206), K278 (= K264)
binding phosphoaminophosphonic acid-adenylate ester: G13 (= G9), K216 (= K206), T236 (= T226), D237 (= D227), C242 (≠ Y232), Y245 (≠ W235), S274 (= S260), M275 (= M261), K278 (= K264)

4olcA Carbamate kinase from giardia lamblia thiocarbamoylated by disulfiram on cys242 (see paper)
41% identity, 95% coverage: 3:301/314 of query aligns to 6:307/308 of 4olcA

query
sites
4olcA

I

V

V

V

I

I

A

A

L

L

G

G

G
|
G

N
|
N

A

A

I

M

L

L

R

Q

R

A

G

K

Q

E

P

K

M

G

T

D

A

Y

D

D

N

T

Q

Q

R

R

E

K

N

N

I

V

R

E

S

I

A

A

Q

S

S

E

I

I

A

Y

T

K

V

I

A

H

E

K

-

A

G

G

N

Y

Q

K

L

V

V

V

I

L

A

T

H

S

G

G

N

N

G

G

P

P

Q

Q

V

V

G

G

L

A

L

I

A

K

L

L

Q

Q

A

N

A

Q

S

A

Y

A

H

A

D

G

V

V

A

S

P

P

-

E

Y

M

P

P

L

L

D

H

V

V

L

C

G

G

A

A

Q

M

T

S

E

Q

A

G

M

F

I

I

G

G

Y

Y

V

M

I

M

E

S

Q

Q

E

A

L

M

G

D

N

N

V

V

L

F

P

C

P

A

E

N

Q

N

P

E

L

P

A

A

T

N

V

C

L

V

T

T

M

C

I

V

E

T

-

Q

-

T

-

L

V

V

A

D

P

P

A

K

D

D

P

Q

A

A

F

F

A

T

N

N

P

P

T

T

K
|
K

P

P

I

V

G

G

P

R

V

F

Y

Y

D

T

R

E

Q

Q

T

E

A

A

-

K

D

D

A

L

M

M

H

A

A

A

A

N

S

P

G

G

W

K

T

I

F

L

A

R

P

E

D

D

-

A

G

G

E

R

H

G

F

W

R

R

R

V

V

V

I

V

A

P

S

S

P

P

K

R

P

P

K

L

R

E

I

I

F

V

E

E

I

Y

G

G

P

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I

R

K

T

T

L

L

V

I

E

D

H

N

G

N

T

V

M

L

V

V

I

I

C

C

A

T

G

N

G

G

I

I

P

P

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C

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K

Y

R

D

E

G

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Q

K

G

-

R

V

L

I

Q

S

G

G

V

V

E

D

A

A

V

V

I

I

D

D

K
|
K

D

D

L

L

A

A

S

T

A

S

L

L

A

A

E

K

Q

T

L

L

H

N

A

S

D

D

M

Y

L

L

V

M

I

I

A

L

T

T

D
|
D

V

V

D
x
L

G

N

V

A

Y

C

T

I

G

-

W

-

G

-

T

-

P

-

D

-

Q

-

R

R

R

K

L

L

S

E

R

E

I

I

T

K

P

L

D

S

E

E

L

I

E

L

T

A

M

L

-

E

-

K

-

D

-

G

H

H

F

F

A

A

G

G

S

S

M

M

G

G

P

P

K
|
K

V

V

E

R

A

A

C

I

R

E

F

F

V

T

R

Q

N

A

T

T

G

G

R

K

P

M

A

S

S

I

I

I

G

T

A

S

L

L

G
x
S

D

T

I

A

A

V

R

D

I

A

A

L

A

N

G

G

Q

K

A

C

G

G

T

T

R

R

V

I

A

I

Q

K

active site: G12 (= G9), N13 (= N10), K130 (= K122), K215 (= K206), K270 (= K264)
binding diethylcarbamodithioic acid: D236 (= D227), L238 (≠ D229), S291 (≠ G285)

2we5A Carbamate kinase from enterococcus faecalis bound to mgadp (see paper)
43% identity, 96% coverage: 2:301/314 of query aligns to 3:309/309 of 2we5A

query
sites
2we5A

R

K

I

M

V

V

I

V

A

A

L

L

G
|
G

N
|
N

A

A

I

I

L

L

R

S

R

N

G

D

Q

A

P

S

M

A

T

H

A

A

D

-

N

Q

Q

Q

R

Q

E

A

N

L

I

V

R

Q

S

T

A

S

A

A

Q

Y

S

L

I

V

A

H

T

L

V

I

A

K

E

Q

G

G

N

H

Q

R

L

L

V

I

I

V

A

S

H

H

G

G

N

N

G

G

P

P

Q

Q

V

V

G

G

-

N

-

L

L

L

A

Q

L

Q

Q

Q

A

A

S

D

Y

S

H

E

D

K

V

N

A

P

P

A

Y

M

P

P

L

L

D

D

V

T

L

C

G

V

A

A

Q

M

T

T

E

Q

A

G

M

S

I

I

G

G

Y

Y

V

W

I

L

E

S

Q

N

E

A

L

L

G

N

N

Q

V

E

L

L

P

N

P

K

-

A

-

G

-

I

E

K

Q

K

P

Q

L

V

A

A

T

T

V

V

L

L

T

T

M

Q

I

V

E

V

V

V

A

D

P

P

A

A

D

D

P

E

A

A

F

F

A

K

N

N

P

P

T

T

K
|
K

P

P

I

I

G

G

P

P

-

F

V

L

Y

T

D

E

R

A

Q

E

T

A

A

K

D

E

A

A

M

M

H

Q

A

A

A

-

S

-

G

G

W

A

T

I

F

F

A

K

P

E

D

D

-

A

G

G

E

R

H

G

F

W

R

R

R

K

V

V

I

V

A

P

S

S

P

P

K

K

P

P

K

I

R

D

I

I

F

H

E

E

I

A

G

E

P

T

I

I

R

N

T

T

L

L

V

I

E

K

H

N

G

D

T

I

M

I

V

T

I

I

C

S

A

C

G

G

I

I

P

P

T

V

V

V

Y

-

D

-

G

G

Q

Q

G

-

R

E

L

L

Q

K

G

G

V

V

E

E

A

A

V

V

I

I

D

D

K
|
K

D

D

L

F

A

A

S

S

A

E

L

K

L

L

A

A

E

E

Q

L

L

V

H

D

A

A

D

D

M

A

L

L

V

V

I

I

A

L

T
|
T

D
x
G

V
|
V

D

D

G

Y

V
|
V

Y
x
C

T

I

G

N

W
x
Y

G

G

T

K

P

P

D

D

Q

E

R

K

R

Q

L

L

S

T

R

N

I

V

T

T

P

V

D

A

E

E

L

L

E

E

T

E

M

Y

-

K

-

Q

-

A

-

G

H

H

F

F

A
|
A

A

P

G

G

S
|
S

M
|
M

G

L

P

P

K
|
K

V

I

E

E

A

A

C

I

R

Q

F

F

V

V

R

E

N

S

T

Q

-

P

G

N

R

K

P

Q

A

A

S

I

I

I

G

T

A

S

L

L

G

E

D

N

I

L

A

G

R

S

I

M

A

S

A

G

G

D

Q

E

-

I

A

V

G

G

T

T

R

V

V

V

A

T

Q

K

active site: G10 (= G9), N11 (= N10), K127 (= K122), K208 (= K206), K270 (= K264)
binding adenosine-5'-diphosphate: G9 (= G8), G10 (= G9), T228 (= T226), G229 (≠ D227), V230 (= V228), V233 (= V231), C234 (≠ Y232), Y237 (≠ W235), A263 (= A257), S266 (= S260), M267 (= M261), K270 (= K264)

2we4A Carbamate kinase from enterococcus faecalis bound to a sulfate ion and two water molecules, which mimic the substrate carbamyl phosphate (see paper)
43% identity, 96% coverage: 2:301/314 of query aligns to 3:309/309 of 2we4A

query
sites
2we4A

R
x
K

I

M

V

V

I

V

A

A

L

L

G
|
G

N
|
N

A

A

I

I

L

L

R

S

R

N

G

D

Q

A

P

S

M

A

T

H

A

A

D

-

N

Q

Q

Q

R

Q

E

A

N

L

I

V

R

Q

S

T

A

S

A

A

Q

Y

S

L

I

V

A

H

T

L

V

I

A

K

E

Q

G

G

N

H

Q
x
R

L

L

V

I

I

V

A

S

H

H

G
|
G

N

N

G
|
G

P

P

Q

Q

V

V

G

G

-

N

-

L

L

L

A

Q

L

Q

Q

Q

A

A

S

D

Y

S

H

E

D

K

V

N

A

P

P

A

Y

M

P

P

L

L

D

D

V

T

L

C

G

V

A

A

Q

M

T

T

E

Q

A

G

M

S

I

I

G

G

Y

Y

V

W

I

L

E

S

Q

N

E

A

L

L

G

N

N

Q

V

E

L

L

P

N

P

K

-

A

-

G

-

I

E

K

Q

K

P

Q

L

V

A

A

T

T

V

V

L

L

T

T

M

Q

I

V

E

V

V

V

A

D

P

P

A

A

D

D

P

E

A

A

F

F

A

K

N

N

P

P

T

T

K
|
K

P

P

I

I

G

G

P

P

-

F

V

L

Y

T

D

E

R

A

Q

E

T

A

A

K

D

E

A

A

M

M

H

Q

A

A

A

-

S

-

G

G

W

A

T

I

F

F

A

K

P

E

D

D

-

A

G

G

E

R

H

G

F

W

R

R

R

K

V

V

I

V

A

P

S

S

P

P

K

K

P

P

K

I

R

D

I

I

F

H

E

E

I

A

G

E

P

T

I

I

R

N

T

T

L

L

V

I

E

K

H

N

G

D

T

I

M

I

V

T

I

I

C

S

A

C

G

G

I

I

P

P

T

V

V

V

Y

-

D

-

G

G

Q

Q

G

-

R

E

L

L

Q

K

G

G

V

V

E

E

A

A

V

V

I

I

D

D

K
|
K

D

D

L

F

A

A

S

S

A

E

L

K

L

L

A

A

E

E

Q

L

L

V

H

D

A

A

D

D

M

A

L

L

V

V

I

I

A

L

T

T

D

G

V

V

D

D

G

Y

V

V

Y

C

T
x
I

G
x
N

W

Y

G

G

T

K

P

P

D

D

Q

E

R

K

R

Q

L

L

S

T

R

N

I

V

T

T

P

V

D

A

E

E

L

L

E

E

T

E

M

Y

-

K

-

Q

-

A

-

G

H
|
H

F
|
F

A

A

A

P

G

G

S

S

M

M

G

L

P

P

K
|
K

V

I

E

E

A

A

C

I

R

Q

F

F

V

V

R

E

N

S

T

Q

-

P

G

N

R

K

P

Q

A

A

S

I

I

I

G

T

A

S

L

L

G

E

D

N

I

L

A

G

R

S

I

M

A

S

A

G

G

D

Q

E

-

I

A

V

G

G

T

T

R

V

V

V

A

T

Q

K

active site: G10 (= G9), N11 (= N10), K127 (= K122), K208 (= K206), K270 (= K264)
binding sulfate ion: K3 (≠ R2), G9 (= G8), G10 (= G9), N11 (= N10), R43 (≠ Q43), G49 (= G49), G51 (= G51), K127 (= K122), K208 (= K206), I235 (≠ T233), N236 (≠ G234), H261 (= H255), F262 (= F256)

P0A2X8 Carbamate kinase 1; EC 2.7.2.2 from Enterococcus faecium (Streptococcus faecium) (see 2 papers)
43% identity, 96% coverage: 2:301/314 of query aligns to 4:310/310 of P0A2X8

query
sites
P0A2X8

R

K

I

M

V

V

I

V

A

A

L

L

G

G

N

N

A

A

I

I

L

L

R

S

R

N

G

D

Q

A

P

S

M

A

T

H

A

A

D

-

N

Q

Q

Q

R

Q

E

A

N

L

I

V

R

Q

S

T

A

S

A

A

Q

Y

S

L

I

V

A

H

T

L

V

I

A

K

E

Q

G

G

N

H

Q

R

L

L

V

I

I

V

A

S

H

H

G

G

N

N

G

G

P

P

Q

Q

V

V

G

G

-

N

-

L

L

L

A

Q

L

Q

Q

Q

A

A

S

D

Y

S

H

E

D

K

V

N

A

P

P

A

Y

M

P

P

L

L

D

D

V

T

L

C

G

V

A

A

Q

M

T

T

E

Q

A

G

M

S

I

I

G

G

Y

Y

V

W

I

L

E

S

Q

N

E

A

L

L

G

N

N

Q

V

E

L

L

P

N

P

K

-

A

-

G

-

I

E

K

Q

K

P

Q

L

V

A

A

T

T

V

V

L

L

T

T

M

Q

I

V

E

V

V

V

A

D

P

P

A

A

D

D

P

E

A

A

F

F

A

K

N

N

P

P

T

T

K

K

P

P

I

I

G

G

P

P

-

F

V

L

Y

T

D
x
E

R

A

Q
x
E

T

A

A
x
K

D
x
E

A

A

M

M

H

Q

A

A

A

-

S

-

G

G

W

A

T

I

F

F

A

K

P

E

D

D

-

A

G

G

E

R

H

G

F

W

R

R

R

K

V

V

I

V

A

P

S

S

P

P

K

K

P

P

K

I

R

D

I

I

F

H

E

E

I

A

G

E

P

T

I

I

R

N

T

T

L

L

V

I

E

K

H

N

G

D

T

I

M

I

V

T

I

I

C

S

A

C

G

G

I

I

P

P

T

V

V

V

Y

-

D

-

G

G

Q

Q

G

-

R

E

L

L

Q

K

G

G

V

V

E

E

A

A

V

V

I

I

D
|
D

K

K

D
|
D

L

F

A

A

S

S

A

E

L

K

L

L

A

A

E

E

Q

L

L

V

H

D

A

A

D

D

M

A

L

L

V

V

I

I

A

L

T

T

D

G

V

V

D

D

G

Y

V

V

Y

C

T

I

G

N

W

Y

G

G

T

K

P

P

D

D

Q

E

R

K

R

Q

L

L

S

T

R

N

I

V

T

T

P

V

D

A

E

E

L

L

E

E

T

E

M

Y

-

K

-

Q

-

A

-

G

H

H

F

F

A

A

A

P

G

G

S

S

M

M

G

L

P

P

K

K

V

I

E

E

A

A

C

I

R

Q

F

F

V

V

R

E

N

S

T

Q

-

P

G

N

R

K

P

Q

A

A

S

I

I

I

G

T

A

S

L

L

G

E

D

N

I

L

A

G

R

S

I

M

A

S

A

G

G

D

Q

E

-

I

A

V

G

G

T

T

R

V

V

V

A

T

Q

K

E136 (≠ D129) mutation to A: No change in activity; when associated with A-138; A-140 and A-141.
E138 (≠ Q131) mutation to A: No change in activity; when associated with A-136; A-140 and A-141.
K140 (≠ A133) mutation to A: No change in activity; when associated with A-136; A-138 and A-141.
E141 (≠ D134) mutation to A: No change in activity; when associated with A-136; A-138 and A-140.
D208 (= D205) mutation to A: Almost no activity; when associated with A-210.
D210 (= D207) mutation to A: Almost no activity; when associated with A-208.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4jz7A Carbamate kinase from giardia lamblia bound to amp-pnp (see paper)
38% identity, 95% coverage: 3:301/314 of query aligns to 7:284/285 of 4jz7A

query
sites
4jz7A

I

V

V

V

I

I

A

A

L

L

G
|
G

N
|
N

A

A

I

M

L

L

R

Q

R

A

G

K

Q

E

P

K

M

G

T

D

A

Y

D

D

N

T

Q

Q

R

R

E

K

N

N

I

V

R

E

S

I

A

A

Q

S

S

E

I

I

A

Y

T

K

V

I

A

H

E

K

-

A

G

G

N

Y

Q

K

L

V

V

V

I

L

A

T

H

S

G

G

N

N

G

G

P

P

Q

Q

V

V

G

G

L

A

L

I

A

K

L

L

Q

Q

A

N

A

Q

S

A

Y

A

H

A

D

G

V

V

A

S

P

P

-

E

Y

M

P

P

L

L

D

H

V

V

L

C

G

G

A

A

Q

M

T

S

E

Q

A

G

M

F

I

I

G

G

Y

Y

V

M

I

M

E

S

Q

Q

E

A

L

M

G

D

N

N

V

V

L

F

P

C

P

A

E

N

Q

N

P

E

L

P

A

A

T

N

V

C

L

V

T

T

M

C

I

V

E

T

-

Q

-

T

-

L

V

V

A

D

P

P

A

K

D

D

P

Q

A

A

F

F

A

T

N

N

P

P

T

T

K
|
K

P

P

I

V

G

-

P

-

V

-

Y

-

D

-

R

-

Q

-

T

-

A

-

D

-

A

-

M

-

H

-

A

-

S

-

G

-

W

-

T

-

F

-

A

-

P

-

D

-

G

-

E

-

H

-

F

-

R

-

V

V

I

V

A

P

S

S

P

P

K

R

P

P

K

L

R

E

I

I

F

V

E

E

I

Y

G

G

P

V

I

I

R

K

T

T

L

L

V

I

E

D

H

N

G

N

T

V

M

L

V

V

I

I

C

C

A

T

G

N

G

G

I

I

P

P

T

C

V

K

Y

R

D

E

G

N

Q

K

G

-

R

V

L

I

Q

S

G

G

V

V

E

D

A

A

V

V

I

I

D

D

K
|
K

D

D

L

L

A

A

S

T

A

S

L

L

A

A

E

K

Q

T

L

L

H

N

A

S

D

D

M

Y

L

L

V

M

I

I

A

L

T
|
T

D
|
D

V

V

D

L

G

N

V

A

Y
x
C

T

I

G

N

W
x
Y

G

K

T

K

P

P

D

D

Q

E

R

R

R

K

L

L

S

E

R

E

I

I

T

K

P

L

D

S

E

E

L

I

E

L

T

A

M

L

-

E

-

K

-

D

-

G

H

H

F

F

A
|
A

A

A

G

G

S
|
S

M
|
M

G

G

P

P

K
|
K

V

V

E

R

A

A

C

I

R

E

F

F

V

T

R

Q

N

A

T

T

G

G

R

K

P

M

A

S

S

I

I

I

G

T

A

S

L

L

G

S

D

T

I

A

A

V

R

D

I

A

A

L

A

N

G

G

Q

K

A

C

G

G

T

T

R

R

V

I

A

I

Q

K

active site: G13 (= G9), N14 (= N10), K131 (= K122), K185 (= K206), K247 (= K264)
binding phosphoaminophosphonic acid-adenylate ester: G12 (= G8), G13 (= G9), K185 (= K206), T205 (= T226), D206 (= D227), C211 (≠ Y232), Y214 (≠ W235), A240 (= A257), S243 (= S260), M244 (= M261), K247 (= K264)

4axsA Structure of carbamate kinase from mycoplasma penetrans (see paper)
34% identity, 95% coverage: 2:299/314 of query aligns to 1:289/291 of 4axsA

query
sites
4axsA

R

R

I

I

V

V

I

I

A

A

L

L

G

G

G
|
G

N
|
N

A

A

I

L

L

-

R

-

G

-

Q

-

P

-

M

-

T

-

A

G

D

D

N

N

-

P

-

S

-

Q

Q

Q

R

K

E

E

N

L

I

V

R

K

S

I

A

P

A

A

Q

A

S

K

I

I

A

A

T

A

-

L

V

I

A

Q

E

E

G

G

N

H

Q

E

L

V

V

I

I

V

A

G

H

H

G
|
G

N

N

G

G

P

P

Q

Q

V

V

G

G

L

M

L

I

-

F

-

N

-

A

-

F

A

A

L

D

Q

A

A

K

S

A

Y

N

H

E

D

K

V

T

A

A

P

L

Y

V

P

P

L

F

D

A

V

E

L

A

G

G

A

G

Q

M

T

S

E

Q

A

G

M

Y

I

I

G

G

Y

Y

V

H

I

M

E

L

Q

T

E

A

L

I

G

S

N

N

V

E

L

L

P

K

-

L

-

N

-

I

E

Q

Q

K

P

D

L

V

A

L

T

Y

V

F

L

L

T

T

M

Q

I

T

E

I

V

V

A

D

P

A

A

N

D

D

P

P

A

A

F

F

A

K

N

N

P

P

T

T

K
|
K

P

P

I

V

G

G

P

P

V

F

Y

Y

D

S

R

N

Q

P

T

N

A

S

D

V

A

I

M

V

H

-

A

-

S

-

G

-

W

-

T

-

F

-

A

-

P

-

D

-

G

-

E

-

H

-

F

-

R

-

R

K

V

V

I

V

A

A

S

S

P

P

K

I

P

P

K

V

R

D

I

F

F

I

E

G

I

I

G

D

P

A

I

I

R

K

T

Q

L

N

V

V

E

N

H

N

G

G

T

C

M

V

V

C

I

I

C

V

A

G

G

G

I

I

P

P

T

T

V

I

Y

I

D

Q

G

D

Q

N

G

Q

R

Y

L

I

Q

-

G

G

V

V

E

D

A

G

V

V

I

I

D

D

K
|
K

D

D

L

F

A

A

S

L

A

A

L

K

L

I

A

A

E

D

Q

A

L

V

H

N

A

A

D

D

M

I

L

F

V

V

I

V

A

L

T

T

D

A

V

V

D

D

G

Y

V

V

Y

Y

T

V

G

D

W

F

G

N

T

K

P

P

D

T

Q

Q

R

K

R

A

L

L

S

K

R

T

I

V

T

D

P

V

D

K

E

A

L

L

E

N

T

N

M

F

-

I

-

N

-

Q

-

D

H

Q

F

F

A

A

A

K

G

G

S

S

M

M

G

L

P

P

K
|
K

V

I

E

K

A

A

C

M

R

G

F

F

V

V

R

N

-

G

N

H

T

P

G

N

R

R

P

S

A

A

S

I

I

I

G

A

A

D

L

L

G

S

D

K

I

V

A

E

R

D

I

A

A

L

A

K

G

G

Q

L

A

S

G

G

T

T

R

K

V

I

active site: G8 (= G9), N9 (= N10), K124 (= K122), K191 (= K206), K253 (= K264)
binding sulfate ion: G8 (= G9), G44 (= G49), K191 (= K206)

2btyA Acetylglutamate kinase from thermotoga maritima complexed with its inhibitor arginine (see paper)
41% identity, 22% coverage: 204:273/314 of query aligns to 179:246/282 of 2btyA

query
sites
2btyA

I

I

D
x
N

K

A

D
|
D

L

T

A

A

S

A

A

A

L

E

L

I

A

A

E

K

Q

S

L

L

H

M

A

A

D

E

M
x
K

L

L

V

I

I

L

A

L

T

T

D

D

V

V

D

D

G

G

V

V

Y

L

T

-

G

-

W

-

G

-

T

-

P

K

D

D

Q

G

R

K

R

L

L

I

S
|
S

R

T

I

L

T

T

P

P

D

D

E

E

L

A

E

E

T

E

M

L

-

I

-

R

-

D

H

G

F

T

A

V

A

T

G

G

S

G

M

M

G

I

P

P

K
|
K

V

V

E

E

A

C

A

A

C

V

R

S

F

A

V

V

R

R

active site: D182 (= D207), K237 (= K264)
binding arginine: K196 (≠ M221), S214 (= S244)
binding n-acetyl-l-glutamate: N180 (≠ D205), D182 (= D207)

Sites not aligning to the query:

active site: 27, 30, 63
binding arginine: 15, 19, 266, 267, 270, 271, 274, 276
binding n-acetyl-l-glutamate: 29, 30, 61, 62

Q9X2A4 Acetylglutamate kinase; N-acetyl-L-glutamate 5-phosphotransferase; NAG kinase; NAGK; EC 2.7.2.8 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
41% identity, 22% coverage: 204:273/314 of query aligns to 179:246/282 of Q9X2A4

query
sites
Q9X2A4

I

I

D

N

K

A

D

D

L

T

A

A

S

A

A

A

L

E

L

I

A

A

E

K

Q

S

L

L

H

M

A

A

D

E

M
x
K

L

L

V

I

I

L

A

L

T

T

D

D

V

V

D

D

G

G

V

V

Y

L

T

-

G

-

W

-

G

-

T

-

P

K

D

D

Q

G

R

K

R

L

L

I

S
|
S

R

T

I

L

T

T

P

P

D

D

E

E

L

A

E

E

T

E

M

L

-

I

-

R

-

D

H

G

F

T

A

V

A

T

G

G

S

G

M

M

G

I

P

P

K

K

V

V

E

E

A

C

A

A

C

V

R

S

F

A

V

V

R

R

K196 (≠ M221) binding L-arginine
S214 (= S244) binding L-arginine

Sites not aligning to the query:

178 binding substrate
266:269 binding L-arginine

2bmuB Ump kinase from pyrococcus furiosus complexed with its substrate ump and its substrate analog amppnp (see paper)
47% identity, 14% coverage: 207:251/314 of query aligns to 122:168/226 of 2bmuB

query
sites
2bmuB

D
|
D

L

A

A

V

S

A

A

A

L

L

A

A

E

E

Q

F

L

L

H

K

A

A

D

D

M

L

L

L

V

V

I

V

A

I

T
|
T

D
x
N

V

V

D

D

G

G

V
|
V

Y
|
Y

T

T

G
x
A

-
x
D

-
x
P

W

K

G

K

T

D

P

P

D

T

Q

A

R

K

L

I

S

K

R

K

I

M

T

K

P

P

D

E

E

E

L

L

binding phosphoaminophosphonic acid-adenylate ester: T141 (= T226), N142 (≠ D227), V146 (= V231), Y147 (= Y232), A149 (≠ G234), D150 (vs. gap), P151 (vs. gap)
binding magnesium ion: D122 (= D207), D122 (= D207)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 9, 10, 44, 45, 46, 182, 183, 184
binding magnesium ion: 7, 183, 184
binding uridine-5'-monophosphate: 44, 45, 67, 70, 71, 114, 115, 116, 119, 120, 121, 179

Q8U122 Uridylate kinase; UK; Uridine monophosphate kinase; UMP kinase; UMPK; EC 2.7.4.22 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) (see paper)
47% identity, 14% coverage: 207:251/314 of query aligns to 121:167/225 of Q8U122

query
sites
Q8U122

D
|
D

L

A

A

V

S

A

A

A

L

L

A

A

E

E

Q

F

L

L

H

K

A

A

D

D

M

L

L

L

V

V

I

V

A

I

T

T

D

N

V

V

D

D

G

G

V

V

Y

Y

T

T

G

A

-

D

-

P

W

K

G

K

T

D

P

P

D

T

Q

A

R

K

L

I

S

K

R

K

I

M

T

K

P

P

D

E

E

E

L

L

D121 (= D207) binding Mg(2+); binding Mg(2+)

Sites not aligning to the query:

6 binding Mg(2+)
44 binding UMP
66 binding UMP
114:120 binding UMP
120 binding Mg(2+)
179 binding UMP
182 binding Mg(2+)

2ji5A Structure of ump kinase from pyrococcus furiosus complexed with utp
47% identity, 14% coverage: 207:251/314 of query aligns to 123:169/219 of 2ji5A

query
sites
2ji5A

D

D

L

A

A

V

S

A

A

A

L

L

A

A

E

E

Q

F

L

L

H

K

A

A

D

D

M

L

L

L

V

V

I

V

A

I

T

T

D

N

V

V

D

D

G

G

V

V

Y

Y

T

T

G

A

-

D

-

P

W

K

G

K

T

D

P

P

D

T

Q

A

R

K

L

I

S

K

R

K

I

M

T

K

P

P

D

E

E

E

L

L

Sites not aligning to the query:

binding uridine 5'-triphosphate: 68, 71, 72, 115, 116, 117, 120, 121, 122

5yeiC Mechanistic insight into the regulation of pseudomonas aeruginosa aspartate kinase (see paper)
36% identity, 38% coverage: 148:265/314 of query aligns to 96:209/397 of 5yeiC

query
sites
5yeiC

G

G

E

N

H

Q

F

V

R

R

R

I

V

L

I

T

A

D

S

S

P

A

K

H

P

T

K

K

-

A

R

R

I

I

F

L

E

H

I

I

G

D

P

D

-

T

-

H

I

I

R

R

T

A

L

D

V

L

E

K

H

A

G

G

T

R

M

V

V

V

I

V

C

V

A

A

G

G

G

F

G

Q

G

G

I

V

P

-

T

-

V

-

Y

-

D

D

G

G

Q

N

G

G

R

N

L

I

Q

T

G

T

V

L

E

-

A

G

V

R

I

G

D

G

K

S

D

D

L

T

A

T

S

G

A

V

L

A

L

L

A

A

E

A

Q

A

L

L

H

K

A

A

D

D

M

E

L

C

V

Q

I

I

A

Y

T

T

D

D

V

V

D

D

G

G

V

V

Y

Y

T

T

G

T

-

D

-

P

W

R

G

V

T

V

P

P

D

Q

Q

A

R

R

L

L

S

D

R

K

I

I

T

T

P

F

D

E

E

E

L

M

E

L

T

E

M

M

H

-

F

-

A

-

G

A

S

S

M

L

G

G

P

S

K

K

V

V

Sites not aligning to the query:

binding lysine: 342, 345, 346, 347, 348, 349, 350
binding threonine: 265, 266, 269, 288, 362, 363

Query Sequence

>WP_011778652.1 NCBI__GCF_000015305.1:WP_011778652.1
MRIVIALGGNAILRRGQPMTADNQRENIRSAAQSIATVAEGNQLVIAHGNGPQVGLLALQ
AASYHDVAPYPLDVLGAQTEAMIGYVIEQELGNVLPPEQPLATVLTMIEVAPADPAFANP
TKPIGPVYDRQTADAMHAASGWTFAPDGEHFRRVIASPKPKRIFEIGPIRTLVEHGTMVI
CAGGGGIPTVYDGQGRLQGVEAVIDKDLASALLAEQLHADMLVIATDVDGVYTGWGTPDQ
RRLSRITPDELETMHFAAGSMGPKVEAACRFVRNTGRPASIGALGDIARIAAGQAGTRVA
QSSEGSPPEERCCT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory