SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011779312.1 NCBI__GCF_000015305.1:WP_011779312.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O53289 Phosphoserine phosphatase SerB2; PSP; PSPase; O-phosphoserine phosphohydrolase; Protein-serine/threonine phosphatase; EC 3.1.3.3; EC 3.1.3.16 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
79% identity, 96% coverage: 15:418/419 of query aligns to 4:407/409 of O53289

query
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O53289

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G18 (= G29) mutation to A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.
G108 (≠ E119) mutation to A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.
D185 (= D196) mutation to G: Completely abolishes enzymatic activity.; mutation to N: Completely abolishes enzymatic activity.
V186 (= V197) mutation to Q: Decreases enzymatic activity by 50%.
D187 (= D198) mutation to N: Decreases enzymatic activity by 15%.
S188 (= S199) mutation to A: No effect on enzymatic activity.
S273 (= S284) mutation to A: Completely abolishes enzymatic activity (PubMed:25521849). Decreases enzymatic activity by 60% (PubMed:25037224).
K318 (= K329) mutation to A: Decreases enzymatic activity by 50%.; mutation to E: Completely abolishes enzymatic activity.
D341 (= D352) mutation to G: Decreases enzymatic activity by 80%.; mutation to N: Decreases enzymatic activity by 85%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-345.
D345 (= D356) mutation to N: Decreases enzymatic activity by 55%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-341.

A0QJI1 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Mycobacterium avium (strain 104) (see paper)
78% identity, 97% coverage: 11:418/419 of query aligns to 2:409/411 of A0QJI1

query
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A0QJI1

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D187 (= D196) binding
D189 (= D198) binding
D343 (= D352) binding

8a21A Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with phenylimidazole (see paper)
78% identity, 94% coverage: 15:409/419 of query aligns to 2:396/396 of 8a21A

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8a21A

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binding magnesium ion: D183 (= D196), D185 (= D198), D339 (= D352)
binding 4-phenyl-1h-imidazole: D13 (= D26), T18 (= T31), Q37 (= Q50), D185 (= D198), E192 (= E205), V193 (= V206), I194 (= I207), T211 (= T224), M215 (= M228), F221 (= F234), R228 (= R241), G273 (= G286)

8a1zA Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with 1-(2,4-dichlorophenyl)-3-hydroxyurea (see paper)
78% identity, 94% coverage: 15:409/419 of query aligns to 2:396/396 of 8a1zA

query
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8a1zA

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E

Q

Q

V

V

I

I

R

R

G

H

R

R

L

L

T

T

L

L

G

G

V

V

L

L

V

V

A

C

A

P

T

A

E

D

V

V

A

A

A

D

G

G

A

P

E

A

L

L

R

R

S

H

E

D

V

V

E

E

A

A

I

I

H

R

G

K

V

V

G

G

L

L

D

D

V

V

S

S

I

I

E

E

S

R

S

S

D

D

G

D

L

V

P

P

V

I

L

I

R

R

V

E

P

P

S

S

T

T

H

H

T

T

I

I

V

F

V

V

L

L

G

G

R

R

P

P

I

I

T

T

A

A

E

A

A

A

F

F

G

G

V

A

V

V

A

A

R

R

E

E

A

V

A

A

L

L

G

G

V

V

N

N

I

I

D

D

F

L

I

I

R

R

G

G

V

V

S

S

D

D

Y

Y

P

P

V

V

T

I

G

G

L

L

E

E

L

L

R

R

V

V

S

S

V

V

P

P

G

G

A

A

Y

D

R

E

E

A

L

L

Q

R

A

T

V

A

L

L

A

N

R

R

V

V

A

S

V

S

E

E

S

H

V

V

D

D

I

V

A

A

V

V

E

E

D

D

Y

Y

S

T

L

L

S

E

R

R

A

A

K

K

R

R

L

L

I

I

V

V

F

F

D
|
D

V

V

D
|
D

S

S

T

T

L

L

I

V

Q

Q

G

G

E
|
E

V

V

I

I

E

E

M

M

L

L

A

A

H

K

A

A

G

G

A

A

E

E

A

G

A

Q

V

V

A

A

E

A

V

I

T

T

E

D

A

A

M
|
M

R

R

G

G

E

E

L

L

D

D

F
|
F

A

A

E

Q

S

S

L
|
L

H

Q

R

Q

R
|
R

V

V

A

A

T

T

L

L

A

A

G

G

L

L

P

P

A

A

S

T

V

V

L

I

D

D

D

E

V

V

A

A

D

G

Q

Q

I

L

E

E

L

L

T

M

P

P

G

G

A

A

R

R

T

T

L

L

R

R

T

T

L

L

R

R

L

L

G

G

Y

Y

H

A

C

C

G

G

I

V

V

V

S

S

G
|
G

G

G

F
|
F

R

R

Q

R

V

I

I

I

E

E

P

P

L

L

A

A

H

E

E

E

L

L

M

M

M

L

D

D

F

Y

V

V

A

A

N

N

E

E

L

L

E

E

I

I

V

V

D

D

G

G

K

T

L

L

T

T

G

G

R

R

V

V

G

G

D

P

V

I

D

D

R

R

P

A

G

G

K

K

A

A

K

T

A

A

L

L

R

R

D

E

F

F

A

A

Q

Q

Q

R

A

A

G

G

V

V

P

P

M

M

E

A

Q

Q

T

T

V

V

A

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D

D

I

I

D

D

M

M

L

L

S

A

A

A

G

G

L

L

G

G

V

I

A

A

F

F

N

N

A

A

K

K

P

P

A

A

L

L

R

R

E

E

V

V

A

A

D

D

A

A

S

S

L

L

S

S

Y

H

P

P

Y

Y

L

L

D

D

T

T

L

V

L

L

F

F

I

L

L

L

G

G

I

V

T

T

R

R

G

G

E

E

I

I

E

E

A

A

D

D

A

A

L

I

D

D

binding 1-(2,4-dichlorophenyl)-3-oxidanyl-urea: D185 (= D198), E192 (= E205), M215 (= M228), F221 (= F234), L225 (= L238), R228 (= R241), G272 (= G285), F274 (= F287), D339 (= D352)
binding magnesium ion: D183 (= D196), D185 (= D198), D339 (= D352)

5jlpA Crystal structure of mycobacterium avium serb2 in complex with serine at act domain
78% identity, 94% coverage: 15:409/419 of query aligns to 2:396/396 of 5jlpA

query
sites
5jlpA

R

K

S

V

S

S

L

V

L

L

I

I

T

T

V

V

T

T

G

G

R

V

D
|
D

Q
|
Q

P

P

G

G

V

V

T

T

S

A

A

T

L

L

F

F

E

E

V

V

L

L

S

S

R

R

H

H

R

G

V

V

D

E

L

L

N

N

V

V

E

E

Q

Q

V

V

I

I

R

R

G

H

R

R

L

L

T

T

L

L

G

G

V

V

L

L

V

V

A

C

A

P

T

A

E

D

V

V

A

A

A

D

G

G

A

P

E

A

L

L

R

R

S

H

E

D

V

V

E

E

A

A

I

I

H

R

G

K

V

V

G

G

L

L

D

D

V

V

S

S

I

I

E

E

S

R

S

S

D

D

G

D

L

V

P

P

V

I

L

I

R

R

V

E

P

P

S

S

T

T

H

H

T

T

I

I

V

F

V

V

L

L

G

G

R

R

P

P

I

I

T

T

A

A

E

A

A

A

F

F

G

G

V

A

V

V

A

A

R

R

E

E

A

V

A

A

L

L

G

G

V

V

N

N

I

I

D

D

F

L

I

I

R

R

G

G

V

V

S

S

D

D

Y

Y

P

P

V

V

T

I

G

G

L

L

E

E

L

L

R

R

V

V

S

S

V

V

P

P

G

G

A

A

Y

D

R

G

E

A

L

L

Q

R

A

T

V

A

L

L

A

N

R

R

V

V

A

S

V

S

E

E

S

H

V

V

D

D

I

V

A

A

V

V

E

E

D

D

Y

Y

S

T

L

L

S

E

R

R

A

A

K

K

R

R

L

L

I

I

V

V

F

F

D
|
D

V
|
V

D
|
D

S

S

T

T

L

L

I

V

Q

Q

G

G

E

E

V

V

I

I

E

E

M

M

L

L

A

A

H

K

A

A

G

G

A

A

E

E

A

G

A

Q

V

V

A

A

E

A

V

I

T

T

E

D

A

A

M

M

R

R

G

G

E

E

L

L

D

D

F

F

A

A

E

Q

S

S

L

L

H

Q

R

Q

R

R

V

V

A

A

T

T

L

L

A

A

G

G

L

L

P

P

A

A

S

T

V

V

L

I

D

D

D

E

V

V

A

A

D

G

Q

Q

I

L

E

E

L

L

T

M

P

P

G

G

A

A

R

R

T

T

L

L

R

R

T

T

L

L

R

R

L

L

G

G

Y

Y

H

A

C

C

G

G

I

V

V

V

S

S

G
|
G

G

G

F

F

R

R

Q

R

V

I

I

I

E

E

P

P

L

L

A

A

H

E

E

E

L

L

M

M

M

L

D

D

F

Y

V

V

A

A

N

N

E

E

L

L

E

E

I

I

V

V

D

D

G

G

K

T

L

L

T

T

G

G

R

R

V

V

G

G

D

P

V

I

D

D

R

R

P

A

G

G

K
|
K

A

A

K

T

A

A

L

L

R

R

D

E

F

F

A

A

Q

Q

Q

R

A

A

G

G

V

V

P

P

M

M

E

A

Q

Q

T

T

V

V

A

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D
|
D

I

I

D

D

M

M

L

L

S

A

A

A

G

G

L

L

G

G

V

I

A

A

F

F

N

N

A

A

K

K

P

P

A

A

L

L

R

R

E

E

V

V

A

A

D

D

A

A

S

S

L

L

S

S

Y

H

P

P

Y

Y

L

L

D

D

T

T

L

V

L

L

F

F

I

L

L

L

G

G

I

V

T

T

R

R

G

G

E

E

I

I

E

E

A

A

D

D

A

A

L

I

D

D

active site: D183 (= D196), V184 (= V197), D185 (= D198), G272 (= G285), K316 (= K329), D343 (= D356)
binding magnesium ion: D183 (= D196), D339 (= D352)
binding serine: D13 (= D26), Q14 (= Q27)

1f5sA Crystal structure of phosphoserine phosphatase from methanococcus jannaschii (see paper)
42% identity, 49% coverage: 188:392/419 of query aligns to 2:206/210 of 1f5sA

query
sites
1f5sA

R

K

A

K

K

K

R

K

L

L

I

I

V

L

F

F

D
|
D

V
x
F

D
|
D

S

S

T

T

L

L

I

V

Q

N

G

N

E

E

V

T

I

I

E

D

M

E

L

I

A

A

A

R

H

E

A

A

G

G

A

V

E

E

A

E

V

V

A

K

E

K

V

I

T

T

E

K

A

E

A

A

M

M

R

E

G

G

E

K

L

L

D

N

F

F

A

E

E

Q

S

S

L

L

H

R

R

K

R

R

V

V

A

S

T

L

L

L

A

K

G

D

L

L

P

P

A

I

S

E

V

K

L

V

D

E

D

K

V

A

A

I

D

K

Q

R

I

I

E

T

L

P

T

T

P

E

G

G

A

A

R

E

T

E

T

T

L

I

R

K

T

E

L

L

R

K

R

N

L

R

G

G

Y

Y

H

V

C

V

G

A

I

V

V

V

S
|
S

G
|
G

G

G

F

F

R

D

Q

I

V

A

I

V

E

N

P

K

L

I

A

K

H

E

E

K

L

L

M

G

M

L

D

D

F

Y

V

A

A

F

A

A

N

N

E

R

L

L

E

I

I

V

V

K

D

D

G

G

K

K

L

L

T

T

G

G

R

D

V

V

V

E

G

G

D

E

V

V

V

L

D

K

R

E

P

N

G

A

K
|
K

A

G

K

E

A

I

L

L

R

E

D

K

F

I

A

A

Q

K

Q

I

A

E

G

G

V

I

P

N

M

L

E

E

Q

D

T

T

V

V

A

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D
|
D

I

I

D

S

M

M

L

F

S

K

A

K

A

A

G

G

L

L

G

K

V

I

A

A

F

F

N

C

A

A

K

K

P

P

A

I

L

L

R

K

E

E

V

K

A

A

D

D

A

I

S

C

L

I

S

E

Y

K

P

R

Y

D

L

L

D

R

T

E

L

I

L

L

active site: D10 (= D196), F11 (≠ V197), D12 (= D198), G99 (= G285), K143 (= K329), D170 (= D356)
binding magnesium ion: D10 (= D196), D12 (= D198), D166 (= D352)
binding phosphate ion: D10 (= D196), F11 (≠ V197), D12 (= D198), S98 (= S284), G99 (= G285), K143 (= K329)

1l7nA Transition state analogue of phosphoserine phosphatase (aluminum fluoride complex) (see paper)
42% identity, 49% coverage: 188:392/419 of query aligns to 1:205/209 of 1l7nA

query
sites
1l7nA

R

K

A

K

K

K

R

K

L

L

I

I

V

L

F

F

D
|
D

V
x
F

D
|
D

S

S

T

T

L

L

I

V

Q

N

G

N

E

E

V

T

I

I

E

D

M

E

L

I

A

A

A

R

H

E

A

A

G

G

A

V

E

E

A

E

V

V

A

K

E

K

V

I

T

T

E

K

A

E

A

A

M

M

R

E

G

G

E

K

L

L

D

N

F

F

A

E

E

Q

S

S

L

L

H

R

R

K

R

R

V

V

A

S

T

L

L

L

A

K

G

D

L

L

P

P

A

I

S

E

V

K

L

V

D

E

D

K

V

A

A

I

D

K

Q

R

I

I

E

T

L

P

T

T

P

E

G

G

A

A

R

E

T

E

T

T

L

I

R

K

T

E

L

L

R

K

R

N

L

R

G

G

Y

Y

H

V

C

V

G

A

I

V

V

V

S
|
S

G
|
G

G

G

F

F

R

D

Q

I

V

A

I

V

E

N

P

K

L

I

A

K

H

E

E

K

L

L

M

G

M

L

D

D

F

Y

V

A

A

F

A

A

N

N

E

R

L

L

E

I

I

V

V

K

D

D

G

G

K

K

L

L

T

T

G

G

R

D

V

V

V

E

G

G

D

E

V

V

V

L

D

K

R

E

P

N

G

A

K
|
K

A

G

K

E

A

I

L

L

R

E

D

K

F

I

A

A

Q

K

Q

I

A

E

G

G

V

I

P

N

M

L

E

E

Q

D

T

T

V

V

A

A

V

V

G

G

D
|
D

G

G

A

A

N
|
N

D
|
D

I

I

D

S

M

M

L

F

S

K

A

K

A

A

G

G

L

L

G

K

V

I

A

A

F

F

N

C

A

A

K

K

P

P

A

I

L

L

R

K

E

E

V

K

A

A

D

D

A

I

S

C

L

I

S

E

Y

K

P

R

Y

D

L

L

D

R

T

E

L

I

L

L

active site: D9 (= D196), F10 (≠ V197), D11 (= D198), G98 (= G285), K142 (= K329), D169 (= D356)
binding aluminum fluoride: D9 (= D196), F10 (≠ V197), D11 (= D198), S97 (= S284), K142 (= K329)
binding tetrafluoroaluminate ion: D9 (= D196), F10 (≠ V197), D11 (= D198), S97 (= S284), G98 (= G285), K142 (= K329), N168 (= N355)
binding magnesium ion: D9 (= D196), D11 (= D198), D165 (= D352)

Q58989 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see 3 papers)
42% identity, 49% coverage: 188:392/419 of query aligns to 3:207/211 of Q58989

query
sites
Q58989

R

K

A

K

K

K

R

K

L

L

I

I

V

L

F

F

D
|
D

V

F

D
|
D

S

S

T

T

L

L

I

V

Q

N

G

N

E
|
E

V

T

I

I

E

D

M

E

L

I

A

A

A

R

H

E

A

A

G

G

A

V

E

E

A

E

V

V

A

K

E

K

V

I

T

T

E

K

A

E

A

A

M

M

R

E

G

G

E

K

L

L

D

N

F

F

A

E

E

Q

S

S

L

L

H

R

R

K

R
|
R

V

V

A

S

T

L

L

L

A

K

G

D

L

L

P

P

A

I

S

E

V

K

L

V

D

E

D

K

V

A

A

I

D

K

Q

R

I

I

E

T

L

P

T

T

P

E

G

G

A

A

R

E

T

E

T

T

L

I

R

K

T

E

L

L

R

K

R

N

L

R

G

G

Y

Y

H

V

C

V

G

A

I

V

V

V

S
|
S

G
|
G

G

G

F

F

R

D

Q

I

V

A

I

V

E

N

P

K

L

I

A

K

H

E

E

K

L

L

M

G

M

L

D

D

F

Y

V

A

A

F

A

A

N

N

E

R

L

L

E

I

I

V

V

K

D

D

G

G

K

K

L

L

T

T

G

G

R

D

V

V

V

E

G

G

D

E

V

V

V

L

D

K

R

E

P

N

G

A

K
|
K

A

G

K

E

A

I

L

L

R

E

D

K

F

I

A

A

Q

K

Q

I

A

E

G

G

V

I

P

N

M

L

E

E

Q

D

T

T

V

V

A

A

V

V

G

G

D
|
D

G

G

A

A

N
|
N

D

D

I

I

D

S

M

M

L

F

S

K

A

K

A

A

G

G

L

L

G

K

V

I

A

A

F

F

N

C

A

A

K

K

P

P

A

I

L

L

R

K

E

E

V

K

A

A

D

D

A

I

S

C

L

I

S

E

Y

K

P

R

Y

D

L

L

D

R

T

E

L

I

L

L

D11 (= D196) active site, Nucleophile; binding ; mutation to N: Loss of activity.
D13 (= D198) active site, Proton donor; binding
E20 (= E205) binding
R56 (= R241) binding
SG 99:100 (= SG 284:285) binding
K144 (= K329) binding
D167 (= D352) binding
N170 (= N355) binding

1l7pA Substrate bound phosphoserine phosphatase complex structure (see paper)
42% identity, 48% coverage: 190:392/419 of query aligns to 2:204/208 of 1l7pA

query
sites
1l7pA

K

K

R

K

L

L

I

I

V

L

F

F

D
x
N

V
x
F

D
|
D

S

S

T

T

L

L

I

V

Q

N

G

N

E
|
E

V

T

I

I

E

D

M

E

L

I

A

A

A

R

H

E

A

A

G

G

A

V

E

E

A

E

V

V

A

K

E

K

V

I

T

T

E

K

A

E

A

A

M
|
M

R

E

G

G

E

K

L

L

D

N

F
|
F

A

E

E

Q

S

S

L

L

H

R

R

K

R
|
R

V

V

A

S

T

L

L

L

A

K

G

D

L

L

P

P

A

I

S

E

V

K

L

V

D

E

D

K

V

A

A

I

D

K

Q

R

I

I

E

T

L

P

T

T

P

E

G

G

A

A

R

E

T

E

T

T

L

I

R

K

T

E

L

L

R

K

R

N

L

R

G

G

Y

Y

H

V

C

V

G

A

I

V

V

V

S
|
S

G
|
G

G

G

F

F

R

D

Q

I

V

A

I

V

E

N

P

K

L

I

A

K

H

E

E

K

L

L

M

G

M

L

D

D

F

Y

V

A

A

F

A

A

N

N

E

R

L

L

E

I

I

V

V

K

D

D

G

G

K

K

L

L

T

T

G

G

R

D

V

V

V

E

G

G

D

E

V

V

V

L

D

K

R

E

P

N

G

A

K
|
K

A

G

K

E

A

I

L

L

R

E

D

K

F

I

A

A

Q

K

Q

I

A

E

G

G

V

I

P

N

M

L

E

E

Q

D

T

T

V

V

A

A

V

V

G

G

D

D

G

G

A

A

N

N

D
|
D

I

I

D

S

M

M

L

F

S

K

A

K

A

A

G

G

L

L

G

K

V

I

A

A

F

F

N

C

A

A

K

K

P

P

A

I

L

L

R

K

E

E

V

K

A

A

D

D

A

I

S

C

L

I

S

E

Y

K

P

R

Y

D

L

L

D

R

T

E

L

I

L

L

active site: N8 (≠ D196), F9 (≠ V197), D10 (= D198), G97 (= G285), K141 (= K329), D168 (= D356)
binding phosphoserine: N8 (≠ D196), F9 (≠ V197), D10 (= D198), E17 (= E205), M40 (= M228), F46 (= F234), R53 (= R241), S96 (= S284), G97 (= G285), K141 (= K329)

1l7oA Crystal structure of phosphoserine phosphatase in apo form (see paper)
40% identity, 48% coverage: 190:392/419 of query aligns to 2:196/200 of 1l7oA

query
sites
1l7oA

K

K

R

K

L

L

I

I

V

L

F

F

D

N

V

F

D

D

S

S

T

T

L

L

I

V

Q

N

G

N

E

E

V

T

I

I

E

D

M

E

L

I

A

A

A

R

H

E

A

A

G

G

A

V

E

E

A

E

V

V

A

K

E

K

V

I

T

T

E

-

A

-

M

-

R

-

G

-

E

-

L

-

D

N

F

F

A

E

E

Q

S

S

L

L

H

R

R

K

R

R

V

V

A

S

T

L

L

L

A

K

G

D

L

L

P

P

A

I

S

E

V

K

L

V

D

E

D

K

V

A

A

I

D

K

Q

R

I

I

E

T

L

P

T

T

P

E

G

G

A

A

R

E

T

E

T

T

L

I

R

K

T

E

L

L

R

K

R

N

L

R

G

G

Y

Y

H

V

C

V

G

A

I

V

V

V

S

S

G

G

F

F

R

D

Q

I

V

A

I

V

E

N

P

K

L

I

A

K

H

E

E

K

L

L

M

G

M

L

D

D

F

Y

V

A

A

F

A

A

N

N

E

R

L

L

E

I

I

V

V

K

D

D

G

G

K

K

L

L

T

T

G

G

R

D

V

V

V

E

G

G

D

E

V

V

V

L

D

K

R

E

P

N

G

A

K

K

A

G

K

E

A

I

L

L

R

E

D

K

F

I

A

A

Q

K

Q

I

A

E

G

G

V

I

P

N

M

L

E

E

Q

D

T

T

V

V

A

A

V

V

G

G

D

D

G

G

A

A

N

N

D

D

I

I

D

S

M

M

L

F

S

K

A

K

A

A

G

G

L

L

G

K

V

I

A

A

F

F

N

C

A

A

K

K

P

P

A

I

L

L

R

K

E

E

V

K

A

A

D

D

A

I

S
x
C

L

I

S
x
E

Y

K

P

R

Y

D

L

L

D

R

T

E

L

I

L

L

binding zinc ion: C186 (≠ S382), E188 (≠ S384)

7qplA Crystal structure of phosphoserine phosphatase (serb) from brucella melitensis in complex with phosphate and magnesium
40% identity, 54% coverage: 176:402/419 of query aligns to 68:293/295 of 7qplA

query
sites
7qplA

V

I

D

D

I

V

A

V

V

V

E

Q

D

E

Y

Q

S

E

L

-

S

-

R

R

A

R

K

K

R

K

L

I

I

L

V

I

F

A

D
|
D

V

M

D
|
D

S

S

T

T

L

M

I

I

Q

G

G

Q

E

E

V

C

I

I

E

D

M

E

L

L

A

A

A

E

H

E

A

A

G

G

A

L

E

R

A

D

A

H

V

V

A

A

E

A

V

I

T

T

E

A

A

R

A

A

M

M

R

N

G

G

E

E

L

I

D

A

F

F

A

E

E

P

S

A

L

L

H

R

R

E

R

R

V

V

A

A

T

L

L

L

A

K

G

G

L

L

P

P

A

L

S

S

V

V

L

I

D

D

D

K

V

V

-

I

A

S

D

T

Q

R

I

I

E

T

L

L

T

T

P

P

G

G

A

G

R

P

T

Q

T

L

L

V

R

R

T

T

L

M

R

R

R

K

L

H

G

G

Y

A

H

Y

C

T

G

A

I

L

V

V

S

S

G

G

F

F

R

T

Q

S

V

F

I

T

E

R

P

R

L

I

A

A

H

E

E

M

L

I

M

G

M

F

D

N

F

E

V

E

A

R

A

A

N

N

E

R

L

L

E

-

I

I

V

D

D

D

G

G

-

T

K

R

L

L

T

T

G

G

R

T

V

V

V

A

G

E

D

P

V

I

V

L

D

G

R

R

P

E

G

A

K

K

A

V

K

E

A

K

L

L

R

V

D

E

F

I

A

A

Q

E

Q

R

A

V

G

G

V

L

P

T

M

P

E

E

Q

D

T

A

V

I

A

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D

D

I

L

D

G

M

M

L

I

S

Q

A

L

A

A

G

G

L

T

G

G

V

V

A

A

F

L

N

H

A

A

K

K

P

P

A

A

L

V

R

A

E

A

V

Q

A

A

D

K

A

M

S

R

L

I

S

D

Y

H

P

G

Y

D

L

L

D

T

T

A

L

L

F

Y

I

I

L

Q

G

G

I

Y

T

R

R

K

G

A

E

D

I

F

binding magnesium ion: D86 (= D196), D88 (= D198), D243 (= D352)

3m1yC Crystal structure of a phosphoserine phosphatase (serb) from helicobacter pylori
33% identity, 47% coverage: 190:388/419 of query aligns to 3:200/208 of 3m1yC

query
sites
3m1yC

K

Q

R

K

L

L

I

A

V

V

F

F

D
|
D

V
x
F

D
|
D

S

S

T

T

L

L

I

V

Q

N

G

A

E

E

V

T

I

I

E

E

M

S

L

L

A

A

A

R

H

A

A

W

G

G

A

V

E

F

A

D

A

E

V

V

A

K

E

T

V

I

T

T

E

L

A

K

A

A

M

M

R

N

G

G

E

T

L

-

D

D

F

F

A

H

E

K

S

S

L

L

H

I

R

L

R

R

V

V

A

S

T

K

L

L

A

K

G

N

L

M

P

P

A

L

S

K

V

L

L

A

D

K

D

E

V

V

A

C

D

E

Q

S

I

L

E

P

L

L

T

F

P

E

G

G

A

A

R

L

T

E

T

L

L

V

R

S

T

A

L

L

R

K

R

E

L

K

G

N

Y

Y

H

K

C

V

G

V

I

C

V

F

S

S

G
|
G

G

G

F

F

R

D

Q

L

V

A

I

T

E

N

P

H

L

Y

A

R

H

D

E

L

L

L

M

H

M

L

D

D

F

A

V

A

A

F

A

S

N

N

E

T

L

L

E

I

I

V

V

E

D

N

G

D

K

A

L

L

T

N

G

G

R

L

V

V

V

T

G

G

D

H

V

M

D

F

R

S

P

H

G

S

K
|
K

A

G

K

E

A

M

L

L

R

L

D

V

F

L

A

Q

Q

R

Q

L

A

L

G

N

V

I

P

S

M

K

E

T

Q

N

T

T

V

L

A

V

V

V

G

G

D
|
D

G

G

A

A

N

N

D
|
D

I

L

D

S

M

M

L

F

S

K

A

H

A

A

G

H

L

I

G

K

V

I

A

A

F

F

N

N

A

A

K

K

P

E

A

V

L

L

R

K

E

Q

V

H

A

A

D

T

A

H

S

C

L

I

S

N

Y

E

P

P

Y

D

L

L

active site: D9 (= D196), F10 (≠ V197), D11 (= D198), G97 (= G285), K141 (= K329), D168 (= D356)
binding magnesium ion: D9 (= D196), D11 (= D198), D164 (= D352)

1l8oA Molecular basis for the local conformational rearrangement of human phosphoserine phosphatase (see paper)
30% identity, 46% coverage: 193:385/419 of query aligns to 14:209/222 of 1l8oA

query
sites
1l8oA

I

V

V

C

F

F

D
|
D

V
|
V

D
|
D

S

S

T

T

L

V

I

I

Q

R

G

E

E

E

V

G

I

I

E

D

M

E

L

L

A

A

A

K

H

I

A

C

G

G

A

V

E

E

A

D

A

A

V

V

A

S

E

E

V

M

T

T

E

R

A

R

A

A

M

M

R

G

G

G

E

A

L

V

D

P

F

F

A

K

E

A

S

A

L

L

H

T

R

E

R

R

V

L

A

A

T

L

L

I

A

Q

G

P

L

S

P

R

A

E

S

Q

V

V

L

Q

D

R

D

L

V

I

A

A

D

E

Q

Q

-

P

I

P

E

H

L

L

T

T

P

P

G

G

A

I

R

R

T

E

T

L

L

V

R

S

T

R

L

L

R

Q

R

E

L

R

G

N

Y

V

H

Q

C

V

G

F

I

L

V

I

S
|
S

G
|
G

G

G

F

F

R

R

Q

S

V

I

I

V

E

E

P

H

L

V

A

A

H

S

E

K

L

L

M

N

M

I

-

P

-

A

D

T

F

N

V

V

A

F

A

A

N

N

E

R

L

L

E

K

I

F

V

Y

D

-

G

-

K

-

L

F

T

N

G

G

R

E

V

Y

V

A

G

G

D

F

V

D

V

E

D

T

R

Q

P

P

-

T

-

A

-

E

-

S

-

G

G

G

K
|
K

A

G

K

K

A

V

L

I

R

K

D

F

F

L

A

K

Q

E

Q

K

A

-

G

-

V

F

P

H

M

F

E

K

Q

K

T

I

V

I

A

M

V

I

G

G

D

D

G
|
G

A

A

N
x
T

D
|
D

I

M

D

E

M

A

L

C

S

P

A

P

A

A

G

D

L

A

G

F

V

I

A

G

F

F

N

G

A

G

K

N

P

V

A

I

L
x
R

R

Q

E

Q

V

V

A

K

D

D

A

N

S

A

L

K

S

W

Y

Y

active site: D17 (= D196), V18 (= V197), D19 (= D198), G107 (= G285), K155 (= K329), D180 (= D356)
binding phosphate ion: D17 (= D196), D19 (= D198), S106 (= S284), K155 (= K329)
binding serine: G177 (= G353), T179 (≠ N355), R199 (≠ L375)

1l8lA Molecular basis for the local confomational rearrangement of human phosphoserine phosphatase (see paper)
30% identity, 46% coverage: 193:385/419 of query aligns to 14:209/222 of 1l8lA

query
sites
1l8lA

I

V

V

C

F

F

D
|
D

V
|
V

D
|
D

S

S

T

T

L

V

I

I

Q

R

G

E

E

E

V

G

I

I

E

D

M

E

L

L

A

A

A

K

H

I

A

C

G

G

A

V

E

E

A

D

A

A

V

V

A

S

E

E

V

M

T

T

E

R

A

R

A

A

M

M

R

G

G

G

E

A

L

V

D

P

F

F

A

K

E

A

S

A

L

L

H

T

R

E

R

R

V

L

A

A

T

L

L

I

A

Q

G

P

L

S

P

R

A

E

S

Q

V

V

L

Q

D

R

D

L

V

I

A

A

D

E

Q

Q

-

P

I

P

E

H

L

L

T

T

P

P

G

G

A

I

R

R

T

E

T

L

L

V

R

S

T

R

L

L

R

Q

R

E

L

R

G

N

Y

V

H

Q

C

V

G

F

I

L

V

I

S

S

G
|
G

G

G

F

F

R

R

Q

S

V

I

I

V

E

E

P

H

L

V

A

A

H

S

E

K

L

L

M

N

M

I

-

P

-

A

D

T

F

N

V

V

A

F

A

A

N

N

E

R

L

L

E

K

I

F

V

Y

D

-

G

-

K

-

L

F

T

N

G

G

R

E

V

Y

V

A

G

G

D

F

V

D

V

E

D

T

R

Q

P

P

-

T

-

A

-

E

-

S

-

G

G

G

K
|
K

A

G

K

K

A

V

L

I

R

K

D

F

F

L

A

K

Q

E

Q

K

A

-

G

-

V

F

P

H

M

F

E

K

Q

K

T

I

V

I

A

M

V

I

G

G

D
|
D

G
|
G

A

A

N
x
T

D
|
D

I

M

D

E

M

A

L

C

S

P

A

P

A

A

G

D

L

A

G

F

V

I

A

G

F

F

N

G

A

G

K

N

P

V

A

I

L

R

R

Q

E

Q

V

V

A

K

D

D

A

N

S

A

L

K

S

W

Y

Y

active site: D17 (= D196), V18 (= V197), D19 (= D198), G107 (= G285), K155 (= K329), D180 (= D356)
binding d-2-amino-3-phosphono-propionic acid: D17 (= D196), D19 (= D198), G107 (= G285), K155 (= K329), D176 (= D352), G177 (= G353), T179 (≠ N355)

6hyyA Human phosphoserine phosphatase with serine and phosphate (see paper)
30% identity, 46% coverage: 193:385/419 of query aligns to 13:208/221 of 6hyyA

query
sites
6hyyA

I

V

V

C

F

F

D
|
D

V
|
V

D
|
D

S

S

T

T

L

V

I

I

Q

R

G

E

E

E

V

G

I

I

E

D

M

E

L

L

A

A

A

K

H

I

A

C

G

G

A

V

E

E

A

D

A

A

V

V

A

S

E

E

V

M

T

T

E

R

A

R

A

A

M

M

R

G

G

G

E

A

L

V

D

P

F

F

A

K

E

A

S

A

L

L

H

T

R

E

R

R

V

L

A

A

T

L

L

I

A

Q

G

P

L

S

P

R

A

E

S

Q

V

V

L

Q

D

R

D

L

V

I

A

A

D

E

Q

Q

-

P

I

P

E

H

L

L

T

T

P

P

G

G

A

I

R

R

T

E

T

L

L

V

R

S

T

R

L

L

R

Q

R

E

L

R

G

N

Y

V

H

Q

C

V

G

F

I

L

V

I

S
|
S

G
|
G

G

G

F

F

R

R

Q

S

V

I

I

V

E

E

P

H

L

V

A

A

H

S

E

K

L

L

M

N

M

I

-

P

-

A

D

T

F

N

V

V

A

F

A

A

N

N

E

R

L

L

E

K

I

F

V

Y

D

-

G

-

K

-

L

F

T

N

G

G

R

E

V

Y

V

A

G

G

D

F

V

D

V

E

D

T

R

Q

P

P

-

T

-

A

-

E

-

S

-

G

G

G

K
|
K

A

G

K

K

A

V

L

I

R

K

D

L

F

L

A

K

Q

E

Q

K

A

-

G

-

V

F

P

H

M

F

E

K

Q

K

T

I

V

I

A

M

V

I

G

G

D
|
D

G

G

A

A

N

T

D

D

I

M

D

E

M

A

L

C

S

P

A

P

A

A

G

D

L

A

G

F

V

I

A

G

F

F

N

G

A

G

K

N

P

V

A

I

L

R

R

Q

E

Q

V

V

A

K

D

D

A

N

S

A

L

K

S

W

Y

Y

binding magnesium ion: D16 (= D196), D18 (= D198), D175 (= D352)
binding serine: D16 (= D196), V17 (= V197), D18 (= D198), S105 (= S284), G106 (= G285), K154 (= K329)

6hyjB Psph human phosphoserine phosphatase (see paper)
30% identity, 46% coverage: 193:385/419 of query aligns to 17:212/223 of 6hyjB

query
sites
6hyjB

I

V

V

C

F

F

D
|
D

V

V

D
|
D

S

S

T

T

L

V

I

I

Q

R

G

E

E
|
E

V

G

I

I

E

D

M

E

L

L

A

A

A

K

H

I

A

C

G

G

A

V

E

E

A

D

A

A

V

V

A

S

E

E

V

M

T

T

E

R

A

R

A

A

M

M

R

G

G

G

E

A

L

V

D

P

F

F

A

K

E

A

S

A

L

L

H

T

R

E

R

R

V

L

A

A

T

L

L

I

A

Q

G

P

L

S

P

R

A

E

S

Q

V

V

L

Q

D

R

D

L

V

I

A

A

D

E

Q

Q

-

P

I

P

E

H

L

L

T

T

P

P

G

G

A

I

R

R

T

E

T

L

L

V

R

S

T

R

L

L

R

Q

R

E

L

R

G

N

Y

V

H

Q

C

V

G

F

I

L

V

I

S

S

G

G

F

F

R

R

Q

S

V

I

I

V

E

E

P

H

L

V

A

A

H

S

E

K

L

L

M

N

M

I

-

P

-

A

D

T

F

N

V

V

A
x
F

A

A

N

N

E

R

L

L

E

K

I

F

V

Y

D

-

G

-

K

-

L

F

T

N

G

G

R

E

V

Y

V

A

G

G

D

F

V

D

V

E

D

T

R

Q

P

P

-

T

-

A

-

E

-

S

-

G

G

G

K

K

A

G

K

K

A

V

L

I

R

K

D

L

F

L

A

K

Q

E

Q
x
K

A

-

G

-

V

F

P

H

M

F

E

K

Q

K

T

I

V

I

A

M

V

I

G

G

D
|
D

G

G

A

A

N

T

D

D

I

M

D

E

M

A

L

C

S

P

A

P

A

A

G

D

L

A

G

F

V

I

A

G

F

F

N

G

A

G

K

N

P

V

A

I

L
x
R

R

Q

E

Q

V

V

A

K

D

D

A

N

S

A

L

K

S

W

Y

Y

binding calcium ion: D20 (= D196), D22 (= D198), D179 (= D352)
binding phosphoserine: F131 (≠ A304), K168 (≠ Q339)
binding serine: E29 (= E205), R202 (≠ L375)

6q6jB Human phosphoserine phosphatase with substrate analogue homo-cysteic acid (see paper)
30% identity, 46% coverage: 193:385/419 of query aligns to 13:208/217 of 6q6jB

query
sites
6q6jB

I

V

V

C

F

F

D
|
D

V
|
V

D
|
D

S

S

T

T

L

V

I

I

Q

R

G

E

E

E

V

G

I

I

E

D

M

E

L

L

A

A

A

K

H

I

A

C

G

G

A

V

E

E

A

D

A

A

V

V

A

S

E

E

V

M

T

T

E

R

A

R

A

A

M

M

R

G

G

G

E

A

L

V

D

P

F
|
F

A

K

E

A

S

A

L

L

H

T

R

E

R

R

V

L

A

A

T

L

L

I

A

Q

G

P

L

S

P

R

A

E

S

Q

V

V

L

Q

D

R

D

L

V

I

A

A

D

E

Q

Q

-

P

I

P

E

H

L

L

T

T

P

P

G

G

A

I

R

R

T

E

T

L

L

V

R

S

T

R

L

L

R

Q

R

E

L

R

G

N

Y

V

H

Q

C

V

G

F

I

L

V

I

S
|
S

G
|
G

F

F

R

R

Q

S

V

I

I

V

E

E

P

H

L

V

A

A

H

S

E

K

L

L

M

N

M

I

-

P

-

A

D

T

F

N

V

V

A

F

A

A

N

N

E

R

L

L

E

K

I

F

V

Y

D

-

G

-

K

-

L

F

T

N

G

G

R

E

V

Y

V

A

G

G

D

F

V

D

V

E

D

T

R

Q

P

P

-

T

-

A

-

E

-

S

-

G

G

G

K
|
K

A

G

K

K

A

V

L

I

R

K

D

L

F

L

A

K

Q

E

Q

K

A

-

G

-

V

F

P

H

M

F

E

K

Q

K

T

I

V

I

A

M

V

I

G

G

D
|
D

G

G

A

A

N
x
T

D

D

I

M

D

E

M

A

L

C

S

P

A

P

A

A

G

D

L

A

G

F

V

I

A

G

F

F

N

G

A

G

K

N

P

V

A

I

L

R

R

Q

E

Q

V

V

A

K

D

D

A

N

S

A

L

K

S

W

Y

Y

binding calcium ion: D16 (= D196), D18 (= D198), D175 (= D352)
binding (2~{S})-2-azanyl-4-sulfo-butanoic acid: D16 (= D196), V17 (= V197), D18 (= D198), F54 (= F234), S105 (= S284), G106 (= G285), G107 (= G286), K154 (= K329), T178 (≠ N355)

P78330 Phosphoserine phosphatase; PSP; PSPase; L-3-phosphoserine phosphatase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Homo sapiens (Human) (see 4 papers)
30% identity, 46% coverage: 193:385/419 of query aligns to 17:212/225 of P78330

query
sites
P78330

I

V

V

C

F

F

D
|
D

V
|
V

D
|
D

S
|
S

T

T

L

V

I

I

Q

R

G

E

E
|
E

V

G

I

I

E
x
D

M

E

L

L

A
|
A

A

K

H

I

A

C

G

G

A

V

E

E

A

D

A

A

V

V

A

S

E

E

V

M

T

T

E

R

A

R

A

A

M
|
M

R
x
G

G

G

E

A

L

V

D

P

F

F

A

K

E

A

S

A

L

L

H

T

R

E

R
|
R

V

L

A

A

T

L

L

I

A

Q

G

P

L

S

P

R

A

E

S

Q

V

V

L

Q

D

R

D

L

V

I

A

A

D

E

Q

Q

-

P

I

P

E

H

L

L

T

T

P

P

G

G

A

I

R

R

T

E

T

L

L

V

R

S

T

R

L

L

R

Q

R

E

L

R

G

N

Y

V

H

Q

C

V

G

F

I

L

V

I

S
|
S

G
|
G

F

F

R

R

Q

S

V

I

I

V

E

E

P

H

L

V

A

A

H

S

E

K

L

L

M

N

M

I

-

P

-

A

D

T

F

N

V

V

A

F

A

A

N
|
N

E

R

L

L

E

K

I

F

V

Y

D

-

G

-

K

-

L

F

T

N

G

G

R

E

V

Y

V

A

G

G

D

F

V

D

V

E

D

T

R

Q

P

P

-

T

-

A

-

E

-

S

-

G

G

G

K
|
K

A

G

K

K

A

V

L

I

R

K

D

L

F

L

A

K

Q

E

Q

K

A

-

G

-

V

F

P

H

M

F

E

K

Q

K

T

I

V

I

A

M

V

I

G

G

D
|
D

G

G

A

A

N
x
T

D

D

I

M

D

E

M

A

L

C

S

P

A

P

A

A

G

D

L

A

G

F

V

I

A

G

F

F

N

G

A

G

K

N

P

V

A

I

L
x
R

R

Q

E

Q

V

V

A

K

D

D

A

N

S

A

L

K

S

W

Y

Y

D20 (= D196) binding
DVD 20:22 (= DVD 196:198) binding
D22 (= D198) binding
S23 (= S199) mutation to A: Reduces L-phosphoserine phosphatase activity by about 50%.; mutation to T: Reduces L-phosphoserine phosphatase activity by about 80%.
E29 (= E205) mutation to D: Reduces L-phosphoserine phosphatase activity by about 95%.; mutation to Q: Loss of L-phosphoserine phosphatase activity.
D32 (≠ E208) to N: in PSPHD; decreased L-phosphoserine phosphatase activity; dbSNP:rs104894035
A35 (= A211) to T: in PSPHD; decreased L-phosphoserine phosphatase activity
M52 (= M228) binding ; to T: in PSPHD; decreased L-phosphoserine phosphatase activity; dbSNP:rs104894036
G53 (≠ R229) binding
R65 (= R241) mutation R->A,K: Loss of L-phosphoserine phosphatase activity.
SGG 109:111 (= SGG 284:286) binding ; binding
N133 (= N306) mutation to A: Reduces L-phosphoserine phosphatase activity by about 75%.
K158 (= K329) binding ; binding
D179 (= D352) binding
T182 (≠ N355) binding ; binding ; mutation to S: Reduces L-phosphoserine phosphatase activity by about 99%.; mutation to V: Reduces L-phosphoserine phosphatase activity by about 25%.
R202 (≠ L375) mutation to A: Reduces L-phosphoserine phosphatase activity by about 99%.; mutation to K: Reduces L-phosphoserine phosphatase activity by about 95%.

4ap9A Crystal structure of phosphoserine phosphatase from t.Onnurineus in complex with ndsb-201 (see paper)
28% identity, 43% coverage: 190:368/419 of query aligns to 9:174/200 of 4ap9A

query
sites
4ap9A

K

K

R

K

L

V

I

A

V

V

F

I

D
|
D

V
x
I

D
x
E

S

G

T

T

L

L

I

T

Q

D

G

F

E

E

V

F

I

W

E

R

M

E

L

M

A

A

A
x
R

H
x
I

A
x
T

G

G

A

-

E

K

A

R

A

E

V

I

A

E

E

E

V

L

T

L

E

E

A

K

A

G

M
x
L

R

S

G

G

E

E

L

V

D

E

F
x
W

A

L

E

D

S

S

L

L

H

L

R

K

R

R

V

V

A

G

T

L

L

I

A

R

G

G

L

I

P

D

A

E

S

G

V

T

L

F

D

L

D

R

V

T

A

R

D

E

Q

K

I

V

E

N

L

V

T

S

P

P

G

E

A

A

R

R

T

E

T

L

L

V

R

E

T

T

L

L

R

R

R

E

L

K

G

G

Y

F

H

K

C

V

G

V

I

L

V

I

S

S

G
|
G

G

S

F

F

R

E

Q

E

V

V

I

L

E

E

P

P

L

F

A

K

H

E

E

-

L

-

M

L

M

G

D

D

F

E

V

F

A

M

A

A

N

N

E

R

L

A

E

I

I

F

V

E

D

D

G

G

K

K

L

F

T

Q

G

G

R

-

V

I

V

R

G

L

D

R

V

F

V

R

D
|
D

R
x
K

P

G

G

E

K

F

A

L

K

K

A

R

L

F

R

R

D

D

F

-

A

-

Q

-

A

-

G

G

V

F

P

-

M

-

E

-

Q

-

T

I

V

L

A

A

V

M

G

G

D

D

G

G

A
x
Y

N
x
A

D
|
D

I

A

D

K

M

M

L

F

S

E

A

R

A

A

G

D

L

M

G

G

V

I

A

A

active site: D15 (= D196), I16 (≠ V197), E17 (≠ D198), G103 (= G285), K141 (≠ R326), D162 (= D356)
binding 3-pyridinium-1-ylpropane-1-sulfonate: R31 (≠ A212), I32 (≠ H213), T33 (≠ A214), L46 (≠ M228), W52 (≠ F234), D140 (= D325), K141 (≠ R326), Y160 (≠ A354), A161 (≠ N355)

6iuyA Structure of dsgpdh of dunaliella salina (see paper)
25% identity, 41% coverage: 193:363/419 of query aligns to 17:186/585 of 6iuyA

query
sites
6iuyA

I

V

V

C

F

F

D
|
D

V

V

D
|
D

S

R

T

T

L

V

I

T

Q

T

G

D

E

A

V

S

I

V

E

G

M

L

L

L

A

A

A

K

H

F

A

M

G

G

A

I

E

E

A

D

A

E

V

A

A

Q

E

S

V

L

T

T

E

E

A

Q

A

A

M

N

R

R

G

G

E

E

L

I

D

N

F

L

A

T

E

K

S

A

L

F

H

E

R

D

R

R

V

L

A

A

T

K

L

L

A

N

G

F

L

T

P

P

A

T

S

D

V

I

-

D

-

R

-

F

L

L

D

E

V

H

A

P

D

A

Q

H

I

T

E

R

L

L

T

V

P

P

G

G

A

V

R

E

T

N

T

L

L

I

R

A

T

A

L

L

R

K

R

A

L

R

G

G

Y

V

H

E

C

V

G

F

I

L

V

I

S

S

G

G

F

F

R

R

Q

E

V

M

I

A

E

L

P

P

L

I

A

-

H

-

E

-

L

-

M

-

D

-

F

-

V

-

A

-

A

A

N

S

E

H

L

L

E

K

I

I

V

P

D

A

G

K

K

N

L

V

T

F

G

C

R

N

V

T

V

M

G

S

D

W

V

Q

V

L

D

D

R

D

P

H

G

G

K

E

A

P

K

V

A

R

L

L

R

S

D

H

F

F

A

K

Q

S

Q

R

A

A

-

I

-

E

-

R

-

I

-

R

G

K

V

Y

P

P

M

Y

E

N

Q

N

T

I

V

I

A

M

V

V

G

G

D
|
D

G

G

A

F

N

S

D

D

I

L

D

E

M

A

L

M

S

Q

A

G

A

S

binding magnesium ion: D20 (= D196), D22 (= D198), D175 (= D352)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 233, 234, 235, 236, 268, 290, 321, 324, 349, 381, 382, 497, 529, 530, 532

Query Sequence

>WP_011779312.1 NCBI__GCF_000015305.1:WP_011779312.1
MSASQNWPAGSSRDRSSLLITVTGRDQPGVTSALFEVLSRHRVDLLNVEQVVIRGRLTLG
VLVAAATEVAAGAELRSEVEAAIHGVGLDVSIESSDGLPVLRVPSTHTIVVLGRPITAEA
FGVVAREAAALGVNIDFIRGVSDYPVTGLELRVSVPPGAYRELQAVLARVAVEESVDIAV
EDYSLSRRAKRLIVFDVDSTLIQGEVIEMLAAHAGAEAAVAEVTEAAMRGELDFAESLHR
RVATLAGLPASVLDDVADQIELTPGARTTLRTLRRLGYHCGIVSGGFRQVIEPLAHELMM
DFVAANELEIVDGKLTGRVVGDVVDRPGKAKALRDFAQQAGVPMEQTVAVGDGANDIDML
SAAGLGVAFNAKPALREVADASLSYPYLDTLLFILGITRGEIEAADALDGVVRRVEIPD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory