SitesBLAST
Comparing WP_011780511.1 NCBI__GCF_000015305.1:WP_011780511.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 13 hits to proteins with known functional sites (download)
4xfjB Crystal structure of argininosuccinate synthase from mycobacterium thermoresistibile in complex with amppnp and arginine
91% identity, 99% coverage: 2:397/400 of query aligns to 1:396/397 of 4xfjB
- active site: D13 (= D14), R94 (= R95), D123 (= D124), S174 (= S175)
- binding phosphoaminophosphonic acid-adenylate ester: A7 (= A8), Y8 (= Y9), S9 (= S10), T14 (= T15), I34 (= I35), G116 (= G117), C117 (= C118), F127 (= F128)
- binding arginine: Y86 (= Y87), S90 (= S91), R126 (= R127), A183 (= A184), E185 (= E186), E259 (= E260), E269 (= E270), Y271 (= Y272)
P59846 Argininosuccinate synthase; Citrulline--aspartate ligase; EC 6.3.4.5 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see 2 papers)
40% identity, 98% coverage: 4:393/400 of query aligns to 2:391/400 of P59846
- 6:14 (vs. 8:16, 100% identical) binding ATP
- A33 (≠ I35) binding ATP
- G114 (= G117) binding ATP
7k5zA Crystal structure of argininosuccinate synthase from legionella pneumophila philadelphia 1 in complex with anppnp and a substrate analogue arginine
42% identity, 98% coverage: 3:394/400 of query aligns to 4:382/390 of 7k5zA
- active site: D15 (= D14), R95 (= R95), D124 (= D124), S176 (= S175)
- binding phosphoaminophosphonic acid-adenylate ester: A9 (= A8), Y10 (= Y9), S11 (= S10), C37 (≠ I35), G117 (= G117), F128 (= F128)
- binding arginine: Y88 (= Y87), T92 (≠ A92), D124 (= D124), R127 (= R127), S185 (≠ A184), E187 (= E186), E261 (= E260), Y273 (= Y272)
1j20A Crystal structure of thermus thermophilus hb8 argininosuccinate synthetase in complex with product (see paper)
39% identity, 98% coverage: 4:393/400 of query aligns to 2:382/386 of 1j20A
- active site: D12 (= D14), R92 (= R95), D121 (= D124), S168 (= S175)
- binding adenosine monophosphate: A6 (= A8), T13 (= T15), A33 (≠ I35), R92 (= R95), H113 (= H116), G114 (= G117), F125 (= F128)
- binding argininosuccinate: Y84 (= Y87), T88 (≠ S91), A115 (≠ C118), T116 (= T119), G119 (= G122), N120 (= N123), D121 (= D124), R124 (= R127), S177 (≠ A184), E179 (= E186), E253 (= E260), Y265 (= Y272)
1j1zA Crystal structure of thermus thermophilus hb8 argininosuccinate synthetase in complex with substrate (see paper)
39% identity, 98% coverage: 4:393/400 of query aligns to 2:382/386 of 1j1zA
- active site: D12 (= D14), R92 (= R95), D121 (= D124), S168 (= S175)
- binding aspartic acid: A115 (≠ C118), T116 (= T119), G119 (= G122), N120 (= N123), D121 (= D124)
- binding adenosine-5'-triphosphate: A6 (= A8), T13 (= T15), A33 (≠ I35), R92 (= R95), I95 (= I98), H113 (= H116), G114 (= G117), F125 (= F128)
- binding citrulline: Y84 (= Y87), T88 (≠ S91), R124 (= R127), S168 (= S175), M169 (≠ I176), S177 (≠ A184), E179 (= E186), E253 (= E260), Y265 (= Y272)
1kh3A Crystal structure of thermus thermophilus hb8 argininosuccinate synthetase in complex with inhibitor (see paper)
39% identity, 98% coverage: 4:393/400 of query aligns to 2:376/380 of 1kh3A
- active site: D12 (= D14), R92 (= R95), D121 (= D124), S168 (= S175)
- binding phosphoaminophosphonic acid-adenylate ester: A6 (= A8), T13 (= T15), T32 (≠ A34), A33 (≠ I35), H113 (= H116), G114 (= G117), F125 (= F128), S168 (= S175), M169 (≠ I176)
- binding arginine: Y84 (= Y87), T88 (≠ S91), R124 (= R127), S168 (= S175), M169 (≠ I176), D170 (= D177), S177 (≠ A184), E179 (= E186), E253 (= E260), Y265 (= Y272)
- binding aspartic acid: A115 (≠ C118), T116 (= T119), G119 (= G122), N120 (= N123), D121 (= D124)
2nz2A Crystal structure of human argininosuccinate synthase in complex with aspartate and citrulline (see paper)
41% identity, 96% coverage: 5:387/400 of query aligns to 4:392/402 of 2nz2A
- active site: D13 (= D14), R92 (= R95), D121 (= D124), S176 (= S175)
- binding aspartic acid: A115 (≠ C118), T116 (= T119), G119 (= G122), N120 (= N123), D121 (= D124)
- binding citrulline: Y84 (= Y87), T88 (≠ S91), N120 (= N123), R124 (= R127), D178 (= D177), S185 (≠ A184), E187 (= E186), E266 (= E260), Y278 (= Y272)
P00966 Argininosuccinate synthase; Citrulline--aspartate ligase; EC 6.3.4.5 from Homo sapiens (Human) (see 16 papers)
40% identity, 96% coverage: 5:387/400 of query aligns to 7:397/412 of P00966
- V64 (≠ A64) to I: in CTLN1; uncertain significance; dbSNP:rs556297791
- Y87 (= Y87) binding L-citrulline
- T91 (≠ S91) to P: in CTLN1; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs769018733
- S92 (≠ A92) binding L-citrulline
- R95 (= R95) to S: in CTLN1; increased thermal stability; loss of argininosuccinate synthase activity
- P96 (= P96) to H: in CTLN1; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; to L: in CTLN1; decreased thermal stability; decreased affinity for aspartate; decreased affinity for citrulline; loss of argininosuccinate synthase activity; to S: in CTLN1; no effect on thermal stability; decreased argininosuccinate synthase activity
- G117 (= G117) to S: in CTLN1; decreased thermal stability; loss of argininosuccinate synthase activity; dbSNP:rs770944877
- A118 (≠ C118) to T: in CTLN1; decreased thermal stability; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs775305020
- T119 (= T119) binding L-aspartate; to I: in CTLN1; decreased thermal stability; loss of argininosuccinate synthase activity
- N123 (= N123) binding L-aspartate; binding L-citrulline
- D124 (= D124) binding L-aspartate; to N: in CTLN1; loss of argininosuccinate synthase activity; dbSNP:rs936192871
- R127 (= R127) binding L-citrulline; to L: increased thermal stability; loss of argininosuccinate synthase activity; dbSNP:rs201623252; to Q: in CTLN1; increased thermal stability; loss of argininosuccinate synthase activity; dbSNP:rs201623252; to W: in CTLN1; severe clinical course; loss of argininosuccinate synthase activity; dbSNP:rs771794639
- R157 (= R152) to C: in CTLN1; decreased thermal stability; loss of argininosuccinate synthase activity; dbSNP:rs770585183; to H: in CTLN1; loss of argininosuccinate synthase activity; dbSNP:rs121908637
- K165 (≠ E160) modified: N6-acetyllysine; by CLOCK; mutation K->Q,R: Significant loss of acetylation but no decrease in enzyme activity; when associated with Q-176 or R-176.
- K176 (≠ R171) modified: N6-acetyllysine; by CLOCK; mutation K->Q,R: Significant loss of acetylation but no decrease in enzyme activity; when associated with Q-165 or R-165.
- W179 (≠ F174) to R: in CTLN1; mild; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs121908646
- S180 (= S175) binding L-citrulline; to I: in CTLN1; increased thermal stability; loss of argininosuccinate synthase activity; dbSNP:rs121908638; to N: in CTLN1; decreased thermal stability; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs121908638
- S189 (≠ A184) binding L-citrulline
- E191 (= E186) to Q: in CTLN1; loss of argininosuccinate synthase activity
- A192 (≠ T187) to V: in CTLN1; decreased protein abundance
- V263 (= V253) to M: in CTLN1; mild clinical course; no effect on affinity for aspartate; no effect on affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs192838388
- R265 (= R255) to C: in CTLN1; severe clinical course; loss of argininosuccinate synthase activity; dbSNP:rs148918985
- E270 (= E260) binding L-citrulline; to Q: in CTLN1; loss of argininosuccinate synthase activity; dbSNP:rs775163147
- R272 (= R262) to C: in CTLN1; increased thermal stability; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs762387914; to H: in CTLN1; increased thermal stability; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs768215008; to L: in CTLN1; increased thermal stability; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs768215008
- G280 (≠ E270) to R: in CTLN1; loss of argininosuccinate synthase activity
- Y282 (= Y272) binding L-citrulline
- T284 (≠ A274) to I: in CTLN1; mild clinical course; dbSNP:rs886039853
- M302 (≠ L292) to V: in CTLN1; no effect on affinity for aspartate; no effect on affinity for citrulline; decreased argininosuccinate synthase activity
- R304 (= R294) to W: in CTLN1; decreased protein abundance; dbSNP:rs121908642
- G324 (= G314) to S: in CTLN1; loss of argininosuccinate synthase activity; dbSNP:rs121908639
- G347 (= G337) to R: in CTLN1; severe clinical course
- Y359 (≠ A349) to D: in CTLN1; mild clinical course
- G362 (= G352) to V: in CTLN1; mild; no effect on affinity for aspartate; no effect on affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs121908647
- G390 (= G380) to R: in CTLN1; loss of argininosuccinate synthase activity; dbSNP:rs121908641
5us8A 2.15 angstrom resolution crystal structure of argininosuccinate synthase from bordetella pertussis
29% identity, 86% coverage: 3:347/400 of query aligns to 15:371/445 of 5us8A
6e5yA 1.50 angstrom resolution crystal structure of argininosuccinate synthase from bordetella pertussis in complex with amp.
29% identity, 86% coverage: 3:347/400 of query aligns to 11:367/438 of 6e5yA
P0A6E4 Argininosuccinate synthase; Citrulline--aspartate ligase; EC 6.3.4.5 from Escherichia coli (strain K12) (see 4 papers)
28% identity, 93% coverage: 3:372/400 of query aligns to 12:394/447 of P0A6E4
- 17:25 (vs. 8:16, 89% identical) binding ATP
- A43 (≠ I35) binding ATP
- Y99 (= Y87) binding L-citrulline
- G129 (= G117) binding ATP
- T131 (= T119) binding ATP; binding L-aspartate
- N135 (= N123) binding L-aspartate; binding L-citrulline
- D136 (= D124) binding ATP; binding L-aspartate
- R139 (= R127) binding L-citrulline
- S192 (= S175) binding L-citrulline
- D194 (= D177) binding ATP
- T201 (≠ A184) binding L-citrulline
- E203 (= E186) binding L-citrulline
- E280 (= E260) binding L-citrulline
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
1k97A Crystal structure of e. Coli argininosuccinate synthetase in complex with aspartate and citrulline (see paper)
29% identity, 86% coverage: 3:347/400 of query aligns to 11:367/432 of 1k97A