SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011780791.1 NCBI__GCF_000015305.1:WP_011780791.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

A0R083 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
95% identity, 100% coverage: 1:446/446 of query aligns to 1:446/446 of A0R083

query
sites
A0R083

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K363 (= K363) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

P9WN37 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
89% identity, 100% coverage: 1:446/446 of query aligns to 1:446/446 of P9WN37

query
sites
P9WN37

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K363 (= K363) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

8ooxB Glutamine synthetase (see paper)
49% identity, 98% coverage: 8:445/446 of query aligns to 6:436/438 of 8ooxB

query
sites
8ooxB

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S

Q

binding magnesium ion: E129 (= E134), E183 (= E189), E190 (= E196)

8tfkA Glutamine synthetase (see paper)
48% identity, 99% coverage: 6:446/446 of query aligns to 4:439/440 of 8tfkA

query
sites
8tfkA

E

E

F

D

V

V

L

L

R

E

T

A

L

V

E

K

E

E

R

R

D

D

I

V

R

K

F

F

V

I

R

R

L

T

W

Q

F

F

T

T

D

D

V

T

L

L

G

G

Y

I

L

I

K

K

S

S

V

W

A

A

I

I

A

P

P

A

A

E

E

Q

L

L

E

E

G

E

A

A

F

F

E

E

E

N

G

G

I

V

G

M

F

F

D

D

G

G

S

S

I

I

E

Q

G

G

F

F

A

T

R

R

V

I

S

E

E

E

A

S

D

D

M

M

V

K

A

L

R

A

P

L

D

D

P

P

S

S

T

T

F

F

Q

R

V

I

L

L

P

P

W

W

T

R

T

P

S

A

A

T

G

G

N

A

H

-

Y

-

S

V

A

A

R

R

M

I

F

L

C

G

D

D

I

V

T

Y

M

L

P

P

D

D

G

G

S

N

P

P

S

F

W

K

A

G

D

D

S

P

R

R

H

Y

V

V

L

L

R

K

R

T

Q

A

L

I

S

K

K

E

A

A

S

E

D

K

L

M

G

G

F

F

S

S

C

M

Y

N

V

V

H

G

P

P

E
|
E

I

L

E
|
E

F

F

L

L

L

F

K

K

P

L

G

D

P

A

D

N

D

G

G

N

T

P

Q

T

P

T

I

E

P

L

A

T

D

D

N

Q

G

G

Y

Y

F

F

D

D

Q

F

A

A

V

P

H

L

D

D

A

R

A

A

P

Q

N

D

F

V

R

R

H

D

A

I

I

D

D

Y

A

A

L

L

E

E

Q

H

M

M

G

G

I

F

S

Q

V

I

E

E

F

A

S

S

H

H

E
|
E

G

V

A

A

P

P

G

S

Q

Q

Q

H

E
|
E

I

I

D
|
D

L
x
F

R

R

Y
x
F

A

G

D

D

A

V

L

L

S

C

M

T

A

A

D

D

N

N

V

V

M

V

T

T

F

F

R

K

Y

Y

L

V

V

V

K

K

E

S

V

I

A

A

L

Y

A

H

D

K

G

G

V

Y

R

Y

A

A

S

S

F

F

M

M

P

P

K

K

P

P

F

L

A

F

E

G

H

V

P

N

G
|
G

S

S

A

G

M

M

H
|
H

T

S

H
x
N

M

Q

S

S

L

L

F

F

E

K

G

D

D

G

T

K

N

N

A

V

F

F

H

Y

S

D

P

P

D

D

D

T

P

P

L

T

Q

K

L

L

S

S

D

Q

V

D

G

A

K

M

S

Y

F

Y

I

I

A

G

G

G

I

L

L

L

E

K

H

H

A

I

N

R

E

E

I

F

S

T

A

A

V

V

T

T

N

N

Q

P

W

V

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

H

P

G

G

G

Y

E
|
E

A

A

P

P

T

V

A

Y

A

I

S

S

W

W

G

S

A

A

A

Q

N

N

R
|
R

S

S

A

S

L

L

V

I

R
|
R

V

I

P

P

M

A

Y

-

T

-

P

-

H

T

K

R

V

G

S

N

S
x
G

R

T

R
|
R

V

I

E
|
E

V

L

R
|
R

S

C

P

P

D

D

S

P

A

A

C

C

N

N

P

P

Y

Y

L

L

T

A

F

F

A

A

V

L

L

M

L

L

A

R

A

A

G

G

L

L

R

E

G

G

I

I

E

K

K

N

N

K

Y

I

V

D

L

P

G

G

P

E

Q

P

A

T

E

N

D

V

N

N

V

I

W

F

S

H

L

L

T

S

P

D

E

K

E

E

R

R

R

E

A

E

M

R

G

G

Y

I

K

R

E

S

L

L

P

P

G

A

S

D

L

L

G

K

V

E

A

A

L

I

T

D

E

E

M

M

E

K

N

G

S

S

E

K

L

F

V

V

A

K

E

E

A

A

L

L

G

G

E

E

H

H

V

V

F

F

D

S

F

H

F

Y

L

L

R

C

N

A

K

K

R

E

S

M

E

E

W

W

E

D

N

E

Y

Y

R

K

S

A

H

V

V

V

T

H

P

P

F

W

E

E

L

L

K

S

N

R

Y

Y

L

L

S

S

L

M

binding adenosine-5'-diphosphate: E128 (= E132), D194 (= D198), F195 (≠ L199), F197 (≠ Y201), N243 (≠ H247), R312 (= R316), R317 (= R321), G325 (≠ S332), R327 (= R334)
binding magnesium ion: E128 (= E132), E128 (= E132), E130 (= E134), E185 (= E189), E192 (= E196), E192 (= E196), H241 (= H245), E329 (= E336)
binding l-methionine-s-sulfoximine phosphate: E128 (= E132), E130 (= E134), E185 (= E189), E192 (= E196), G237 (= G241), H241 (= H245), R294 (= R298), E300 (= E304), R312 (= R316), R331 (= R338)

8ufjB Glutamine synthetase (see paper)
48% identity, 99% coverage: 6:446/446 of query aligns to 8:443/444 of 8ufjB

query
sites
8ufjB

E

E

F

D

V

V

L

L

R

E

T

A

L

V

E

K

E

E

R

R

D

D

I

V

R

K

F

F

V

I

R

R

L

T

W

Q

F

F

T

T

D

D

V

T

L

L

G

G

Y

I

L

I

K

K

S

S

V

W

A

A

I

I

A

P

P

A

A

E

E

Q

L

L

E

E

G

E

A

A

F

F

E

E

E

N

G

G

I

V

G

M

F

F

D

D

G

G

S

S

I

I

E

Q

G

G

F

F

A

T

R

R

V

I

S

E

E

E

A

S

D

D

M

M

V

K

A

L

R

A

P

L

D

D

P

P

S

S

T

T

F

F

Q

R

V

I

L

L

P

P

W

W

T

R

T

P

S

A

A

T

G

G

N

A

H

-

Y

-

S

V

A

A

R

R

M

I

F

L

C

G

D

D

I

V

T

Y

M

L

P

P

D

D

G

G

S

N

P

P

S

F

W

K

A

G

D

D

S

P

R

R

H

Y

V

V

L

L

R

K

R

T

Q

A

L

I

S

K

K

E

A

A

S

E

D

K

L

M

G

G

F

F

S

S

C

M

Y

N

V

V

H

G

P

P

E
|
E

I

L

E
|
E

F

F

L

L

L

F

K

K

P

L

G

D

P

A

D

N

D

G

G

N

T

P

Q

T

P

T

I

E

P

L

A

T

D

D

N

Q

G

G

Y

Y

F

F

D

D

Q

F

A

A

V

P

H

L

D

D

A

L

A

G

P

Q

N

D

F

V

R

R

H

D

A

I

I

D

D

Y

A

A

L

L

E

E

Q

H

M

M

G

G

I

F

S

Q

V

I

E

E

F

A

S

S

H

H

E
|
E

G

V

A

A

P

P

G

S

Q

Q

Q

H

E
|
E

I

I

D

D

L

F

R

R

Y

F

A

G

D

D

A

V

L

L

S

C

M

T

A

A

D

D

N

N

V

V

M

V

T

T

F

F

R

K

Y

Y

L

V

V

V

K

K

E

S

V

I

A

A

L

Y

A

H

D

K

G

G

V

Y

R

Y

A

A

S

S

F

F

M

M

P

P

K

K

P

P

F

L

A

F

E

G

H

V

P

N

G

G

S

S

A

G

M

M

H
|
H

T

S

H

N

M

Q

S

S

L

L

F

F

E

K

G

D

D

G

T

K

N

N

A

V

F

F

H

Y

S

D

P

P

D

D

D

T

P

P

L

T

Q

K

L

L

S

S

D

Q

V

D

G

A

K

M

S

Y

F

Y

I

I

A

G

G

G

I

L

L

L

E

K

H

H

A

I

N

R

E

E

I

F

S

T

A

A

V

V

T

T

N

N

Q

P

W

V

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

H

P

G

G

G

Y

E

E

A

A

P

P

T

V

A

Y

A

I

S

S

W

W

G

S

A

A

A

Q

N

N

R

R

S

S

A

S

L

L

V

I

R

R

V

I

P

P

M

A

Y

-

T

-

P

-

H

T

K

R

V

G

S

N

S

G

R

T

R

R

V

I

E
|
E

V

L

R

R

S

C

P

P

D

D

S

P

A

A

C

C

N

N

P

P

Y

Y

L

L

T

A

F

F

A

A

V

L

L

M

L

L

A

R

A

A

G

G

L

L

R

E

G

G

I

I

E

K

K

N

N

K

Y

I

V

D

L

P

G

G

P

E

Q

P

A

T

E

N

D

V

N

N

V

I

W

F

S

H

L

L

T

S

P

D

E

K

E

E

R

R

R

E

A

E

M

R

G

G

Y

I

K

R

E

S

L

L

P

P

G

A

S

D

L

L

G

K

V

E

A

A

L

I

T

D

E

E

M

M

E

K

N

G

S

S

E

K

L

F

V

V

A

K

E

E

A

A

L

L

G

G

E

E

H

H

V

V

F

F

D

S

F

H

F

Y

L

L

R

C

N

A

K

K

R

E

S

M

E

E

W

W

E

D

N

E

Y

Y

R

K

S

A

H

V

V

V

T

H

P

P

F

W

E

E

L

L

K

S

N

R

Y

Y

L

L

S

S

L

M

binding magnesium ion: E132 (= E132), E134 (= E134), E189 (= E189), E196 (= E196), H245 (= H245), E333 (= E336)

8oozA Glutamine synthetase (see paper)
48% identity, 98% coverage: 8:445/446 of query aligns to 6:428/430 of 8oozA

query
sites
8oozA

V

I

L

F

R

R

T

I

L

V

E

E

R

K

D

N

I

V

R

R

F

F

V

V

R

R

L

L

W

Q

F

F

T

V

D

D

V

V

L

Q

G

G

Y

I

L

P

K

K

S

N

V

V

A

A

I

I

A

P

P

V

A

G

E

Q

L

L

E

E

G

K

A

A

F

L

E

G

E

P

G

G

I

I

G

H

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F

-

A

-

R

-

V

-

S

-

E

-

A

S

D

D

M

M

V

V

A

L

R

R

P

P

D

D

P

P

S

D

T

T

F

F

Q

R

V

V

L

L

P

P

W

W

T

S

T

T

S

A

A

-

G

-

N

-

H

-

Y

-

S

E

A

A

R

R

M

L

F

I

C

C

D

D

I

I

T

E

M

L

P

P

D

D

G

G

S

K

P

P

S

F

W

M

A

G

D

C

S

P

R

R

H

Q

V

V

L

L

R

K

Q

N

L

M

S

E

K

E

A

A

S

A

D

K

L

L

G

G

F

Y

S

V

C

M

Y

N

V

T

H
x
G

P

P

E
|
E

I

M

E

E

F

F

L

L

L

F

K

K

P

-

G

R

P

Q

D

D

D

G

G

M

T

P

Q

T

P

N

I

I

P

P

A

Q

D

D

N

R

G

G

Y

Y

F

F

D

D

Q

L

A

A

V

P

H

I

D

D

A

L

A

A

P

E

N

E

F

I

R

K

R

R

H

E

A

I

I

V

D

L

A

V

L

L

E

E

Q

E

M

M

G

G

I

F

S

E

V

V

E
|
E

F

A

S

A

H

H

E

E

G

V

A

A

P

F

G

G

Q

Q

Q

H

E

E

I

I

D

D

L
x
F

R
x
K

Y
|
Y

A

D

D

N

A

A

L

L

S

A

M

T

A

A

D

D

N

N

V

V

M

I

T

T

F

L

R

K

Y

Y

L

V

V

A

K

K

E

T

V

L

A

A

L

L

A

Q

D

H

G

G

V

L

R

H

A

A

S

T

F

F

M

M

P

P

K

K

P

P

F

I

A

F

E

G

H

V

P

N

G

G

S

S

A

G

M

M

H
|
H

T

T

H
x
N

M

T

S
|
S

L

L

F

F

E

K

G

D

D

G

T

K

N

N

A

A

F

F

H

Y

S

D

P

P

D

D

D

A

P

P

L

D

Q

Q

L

I

S

S

D

D

V

T

G

L

K

R

S

Y

F

F

I

V

A

G

G

G

I

V

L

L

E

K

H

H

A

I

N

R

E

A

I

I

S

T

A

A

V

I

T

T

N

N

Q

P

W

L

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

H

P

G

G

G

Y

E

E

A

A

P

P

T

V

A

Y

A

I

S

T

W

W

G

S

A

G

A

P

N

N

R

R

S

S

A

S

L

L

V

I

R

R

V

V

P

P

M

A

Y

-

T

-

P

-

H

P

K

R

V

G

S

N

S
|
S

R

T

R
|
R

V

I

E
|
E

V

I

R

R

S

S

P

P

D

D

S

P

A

S

C

C

N

N

P

P

Y

Y

L

L

T

A

F

F

A

A

V

A

L

I

L

L

A

A

G

G

L

L

R

D

G

G

I

V

E

-

K

K

N

N

Y

K

V

I

L

E

G

P

P

P

Q

E

-

R

A

V

E

E

D

K

N

N

V

I

W

Y

S

K

L

L

T

T

P

E

E

E

R

R

R

E

A

K

M

L

G

G

Y

I

K

G

E

M

L

L

P

P

G

G

S

T

L

L

G

K

V

E

A

A

L

I

T

E

E

C

M

F

E

K

N

E

S

D

E

E

L

L

V

L

A

V

E

S

A

A

L

L

G

G

E

E

H

H

V

V

F

S

D

Q

F

S

F

I

L

I

R

N

N

V

K

A

R

M

S

A

E

D

W

W

E

D

N

S

Y

Y

R

R

S

T

H

Q

V

V

T

H

P

Q

F

W

E

E

L

L

K

D

N

R

Y

Y

L

L

S

Q

binding adenosine-5'-triphosphate: G117 (≠ H130), E170 (= E184), F185 (≠ L199), K186 (≠ R200), Y187 (= Y201), N233 (≠ H247), S235 (= S249), S315 (= S332), R317 (= R334)
binding magnesium ion: E119 (= E132), H231 (= H245), E319 (= E336)

7tfaB Glutamine synthetase (see paper)
46% identity, 99% coverage: 6:446/446 of query aligns to 5:440/441 of 7tfaB

query
sites
7tfaB

E

E

F

D

V

I

L

I

R

R

T

I

L

A

E

E

R

E

D

N

I

V

R

R

F

F

V

I

R

R

L

L

W

Q

F

F

T

T

D

D

V

L

L

L

G

G

Y

T

L

I

K

K

S

N

V

V

A

E

I

I

A

P

P

V

A

S

E

Q

L

L

E

E

G

K

A

A

F

L

E

D

E

N

G

K

I

M

G

M

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F
x
Y

A

V

R
|
R

V

I

S

E

E

E

A

S

D

D

M

M

V

Y

A

L

R

Y

P

P

D

D

P

L

S

D

T

T

F

W

Q

V

V

V

L

F

P

P

W

W

T

V

T

T

S

S

A

-

G

-

N

-

H

D

Y

R

S

V

A

A

R

R

M

L

F

I

C

C

D

D

I

I

T

Y

M

K

P

P

D

D

G

G

S

S

P

P

S

F

W

A

A

G

D

D

S

P

R

R

H

G

V

I

L

L

R

K

R

R

Q

V

L

L

S

K

K

E

A

A

S

E

D

E

L

L

G

G

F

Y

-

T

S

S

C

M

Y

N

V

V

H

G

P

P

E
|
E

I

P

E
|
E

F

F

L

L

L

F

K

K

P

T

G

D

P

E

D

K

D

G

G

D

T

P

Q

T

P

T

I

E

P

L

A

N

D

D

N

Q

G

G

Y
|
Y

F

F

D

D

Q

L

A

A

V

P

H

M

D

D

A

L

A

G

P

E

N

N

F

C

R

R

H

E

A

I

I

V

D

L

A

K

L

L

E

E

Q

E

M

M

G

G

I

F

S

E

V

I

E

E

F

A

S

S

H

H

E
|
E

G
x
V

A

A

P

P

G

G

Q

Q

Q

H

E
|
E

I

I

D

D

L

F

R

K

Y

Y

A

A

D

D

A

A

L

V

S

K

M

A

A

A

D

D

N

Q

V

I

M

Q

T

T

F

F

R

K

Y

L

L

V

V

V

K

K

E

T

V

I

A

A

L

R

A

Q

D

H

G

G

V

L

R

H

A

A

S

T

F

F

M

M

P

P

K

K

P

P

F

L

A

F

E

G

H

V

P
x
N

G
|
G

S

S

A

G

M

M

H
|
H

T

C

H

N

M

Q

S

S

L

L

F

F

E

K

G

D

D

N

T

E

N

N

A

V

F

F

H

Y

S

D

P

E

D

T

D

D

P

E

L

L

Q

G

L

L

S

S

D

Q

V

T

G

A

K

R

S

H

F

Y

I

M

A

A

G

G

I

I

L

L

E

K

H

H

A

A

N

R

E

A

I

M

S

A

A

A

V

I

T

T

N

N

Q

P

W

T

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

H

P

G
|
G

G
x
Y

E
|
E

A

A

P

P

T

C

A

Y

A

V

S

A

W

W

G

S

A

A

A

S

N

N

R
|
R

S

S

A

P

L

M

V

I

R

R

V

I

P

P

M

-

Y

-

T

-

P

A

H

S

K

R

V

G

S

L

S

S

R

T

R

R

V

V

E
|
E

V

V

R

R

S

N

P

P

D

D

S

P

A

A

C

A

N

N

P

P

Y

Y

L

L

T

A

F

L

A

A

V

V

L

M

L

L

A

R

A

A

G

G

L

L

R

D

G

G

I

I

E

K

K

R

N

Q

Y

M

V

A

L

L

G

P

P

A

Q

P

A

I

E

D

D

R

N

N

V

I

W

Y

S

V

L

M

T

S

P

E

E

E

R

R

R

I

A

E

M

E

G

G

Y

I

K

P

E

S

L

L

P

P

G

A

S

D

L

L

G

K

V

E

A

A

L

L

T

S

E

E

M

L

E

I

N

R

S

S

E

E

L

V

V

I

A

S

E

D

A

A

L

L

G

G

E

D

H

H

V

A

F

L

D

A

F

Y

F

F

L

Y

R

E

N

L

K

K

R

E

S

I

E

E

W

W

E

D

N
x
M

Y

Y

R

R

S

T

H

Q

V

V

T

H

P

Q

F

W

E

E

L

R

K

D

N

Q

Y

Y

L

L

S

T

L

L

binding glutamine: E131 (= E134), Y153 (= Y156), E186 (= E189), G238 (= G241), H242 (= H245), R295 (= R298), E301 (= E304)
binding magnesium ion: E129 (= E132), E131 (= E134), E186 (= E189), E193 (= E196), H242 (= H245), E330 (= E336)
binding : Y58 (≠ F59), R60 (= R61), V187 (≠ G190), N237 (≠ P240), G299 (= G302), Y300 (≠ G303), R313 (= R316), M424 (≠ N430)

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
46% identity, 99% coverage: 6:445/446 of query aligns to 6:441/443 of 4lnkA

query
sites
4lnkA

E

E

F

D

V

I

L

E

R

K

T

L

L

V

E

K

E

E

R

E

D

N

I

V

R

K

F

Y

V

I

R

R

L

L

W

Q

F

F

T

T

D

D

V

I

L

L

G

G

Y

T

L

I

K

K

S

N

V

V

A

E

I

I

A

P

P

V

A

S

E

Q

L

L

E

G

G
x
K

A

A

F

L

E

D

E

N

G

K

I

V

G
x
M

F

F

D
|
D

G

G

S

S

I

I

E

E

G

G

F

F

A

V

R

R

V

I

S

E

E

E

A

S

D

D

M

M

V

Y

A

L

R

Y

P

P

D

D

P

L

S

N

T

T

F

F

Q

V

V

I

L

F

P

P

W

W

T

T

T

A

S

E

A

K

G

G

N

K

H

-

Y

-

S

V

A

A

R

R

M

F

F

I

C

C

D

D

I

I

T

Y

M

N

P

P

D

D

G

G

S

T

P

P

S

F

W

E

A

G

D

D

S

P

R

R

H

N

V

N

L

L

R

K

R

R

Q

I

L

L

S

K

K

E

A

M

S

E

D

D

L

L

G

G

F

F

S

S

C

D

Y

F

-

N

V

L

H

G

P

P

E
|
E

I

P

E
|
E

F

F

L

L

L

F

K

K

P

L

G

-

P

-

D

D

D

E

G

K

T

G

Q

E

P

P

I

T

P

L

A

E

-

L

-

N

D

D

N

K

G

G

Y

Y

F

F

D

D

Q

L

A

A

V

P

H

T

D

D

A

L

A

G

P

E

N

N

F

C

R

R

H

D

A

I

I

V

D

L

A

E

L

L

E

E

Q

E

M

M

G

G

I

F

S

E

V

I

E

E

F

A

S

S

H

H

E
|
E

G
x
V

A

A

P

P

G

G

Q

Q

Q

H

E
|
E

I

I

D

D

L
x
F

R

K

Y
|
Y

A

A

D

G

A

A

L

V

S

R

M

S

A

C

D

D

N

D

V

I

M

Q

T

T

F

F

R

K

Y

L

L

V

V

V

K

K

E

T

V

I

A

A

L

R

A

K

D

H

G

G

V

L

R

H

A

A

S

T

F

F

M

M

P

P

K

K

P

P

F

L

A

F

E

G

H

V

P
x
N

G
|
G

S

S

A
x
G

M

M

H
|
H

T

C

H
x
N

M

L

S
|
S

L

L

F

F

E

K

G

N

D

G

T

V

N

N

A

A

F

F

H

F

S

D

P

E

D

N

D

A

P

D

L

L

Q

Q

L

L

S

S

D

E

V

T

G

A

K

K

S

H

F

F

I

I

A

A

G

G

I

I

L

V

E

K

H

H

A

A

N

T

E

S

I

F

S

T

A

A

V

V

T

T

N

N

Q

P

W

T

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

H

P

G

G

G

Y

E
|
E

A

A

P

P

T

C

A

Y

A

V

S

A

W

W

G

S

A

A

A

Q

N

N

R
|
R

S

S

A

P

L

L

V

I

R

R

V

I

P

P

M

A

Y

-

T

-

P

-

H
x
S

K

R

V

G

S

I

S
|
S

R

T

R
|
R

V

V

E
|
E

V

V

R
|
R

S

S

P

V

D

D

S

P

A

A

C

A

N

N

P

P

Y

Y

L

L

T

A

F

L

A

S

V

V

L

L

A

A

G

G

L

L

R

D

G

G

I

I

E

-

K

K

N

N

Y

K

V

L

L

E

G

A

P

P

Q

A

A

P

E

I

D

D

-

R

N

N

V

I

W

Y

S

V

L

M

T

S

P

K

E

E

R

R

R

M

A

E

M

N

G

G

Y

I

K

V

E

D

L

L

P

P

G

A

S

T

L

L

G

A

V

E

A

A

L

L

T

E

E

E

M

F

E

K

N

S

S

N

E

E

L

V

V

M

A

V

E

K

A

A

L

L

G

G

E

E

H

H

V

L

F

F

D

E

F

H

F

F

L

I

R

E

N

A

K

K

R

E

S

I

E

E

W

W

E

D

N

M

Y

F

R

R

S

T

H

Q

V

V

T

H

P

P

F

W

E

E

L

R

K

E

N

Q

Y

Y

L

M

S

S

active site: D52 (= D52), E131 (= E132), E133 (= E134), E188 (= E189), E195 (= E196), H244 (= H245), R315 (= R316), E332 (= E336), R334 (= R338)
binding adenosine-5'-diphosphate: K43 (≠ G43), M50 (≠ G50), F198 (≠ L199), Y200 (= Y201), N246 (≠ H247), S248 (= S249), S324 (≠ H328), S328 (= S332), R330 (= R334)
binding glutamic acid: E133 (= E134), E188 (= E189), V189 (≠ G190), N239 (≠ P240), G240 (= G241), G242 (≠ A243), E303 (= E304)
binding magnesium ion: E131 (= E132), E188 (= E189), E195 (= E196), H244 (= H245), E332 (= E336)

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
46% identity, 99% coverage: 6:445/446 of query aligns to 6:441/443 of 4lniA

query
sites
4lniA

E

E

F

D

V

I

L

E

R

K

T

L

L

V

E

K

E

E

R

E

D

N

I

V

R

K

F

Y

V

I

R

R

L

L

W

Q

F

F

T

T

D

D

V

I

L

L

G

G

Y

T

L

I

K

K

S

N

V

V

A

E

I

I

A

P

P

V

A

S

E

Q

L

L

E

G

G

K

A

A

F

L

E

D

E

N

G

K

I

V

G

M

F

F

D
|
D

G

G

S

S

I

I

E

E

G

G

F

F

A

V

R

R

V

I

S

E

E

E

A

S

D

D

M

M

V

Y

A

L

R

Y

P

P

D

D

P

L

S

N

T

T

F

F

Q

V

V

I

L

F

P

P

W

W

T

T

T

A

S

E

A

K

G

G

N

K

H

-

Y

-

S

V

A

A

R

R

M

F

F

I

C

C

D

D

I

I

T

Y

M

N

P

P

D

D

G

G

S

T

P

P

S

F

W

E

A

G

D

D

S

P

R

R

H

N

V

N

L

L

R

K

R

R

Q

I

L

L

S

K

K

E

A

M

S

E

D

D

L

L

G

G

F

F

S

S

C

D

Y

F

-

N

V

L

H

G

P

P

E
|
E

I

P

E
|
E

F

F

L

L

L

F

K

K

P

L

G

-

P

-

D

D

D

E

G

K

T

G

Q

E

P

P

I

T

P

L

A

E

-

L

-

N

D

D

N

K

G

G

Y

Y

F

F

D

D

Q

L

A

A

V

P

H

T

D

D

A

L

A

G

P

E

N

N

F

C

R

R

H

D

A

I

I

V

D

L

A

E

L

L

E

E

Q

E

M

M

G

G

I

F

S

E

V

I

E
|
E

F

A

S

S

H

H

E
|
E

G

V

A

A

P

P

G

G

Q

Q

Q

H

E
|
E

I

I

D
|
D

L

F

R

K

Y
|
Y

A

A

D

G

A

A

L

V

S

R

M

S

A

C

D

D

N

D

V

I

M

Q

T

T

F

F

R

K

Y

L

L

V

V

V

K

K

E

T

V

I

A

A

L

R

A

K

D

H

G

G

V

L

R

H

A

A

S

T

F

F

M

M

P

P

K

K

P

P

F

L

A

F

E

G

H

V

P

N

G

G

S

S

A

G

M

M

H
|
H

T

C

H
x
N

M

L

S
|
S

L

L

F

F

E

K

G

N

D

G

T

V

N

N

A

A

F

F

H

F

S

D

P

E

D

N

D

A

P

D

L

L

Q

Q

L

L

S

S

D

E

V

T

G

A

K

K

S

H

F

F

I

I

A

A

G

G

I

I

L

V

E

K

H

H

A

A

N

T

E

S

I

F

S

T

A

A

V

V

T

T

N

N

Q

P

W

T

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

H

P

G

G

G

Y

E
|
E

A

A

P

P

T

C

A

Y

A

V

S

A

W

W

G

S

A

A

A

Q

N

N

R
|
R

S

S

A

P

L

L

V

I

R
|
R

V

I

P

P

M

A

Y

-

T

-

P

-

H

S

K

R

V

G

S

I

S

S

R

T

R
|
R

V

V

E
|
E

V

V

R
|
R

S

S

P

V

D

D

S

P

A

A

C

A

N

N

P

P

Y

Y

L

L

T

A

F

L

A

S

V

V

L

L

A

A

G

G

L

L

R

D

G

G

I

I

E

-

K

K

N

N

Y

K

V

L

L

E

G

A

P

P

Q

A

A

P

E

I

D

D

-

R

N

N

V

I

W

Y

S

V

L

M

T

S

P

K

E

E

R

R

R

M

A

E

M

N

G

G

Y

I

K

V

E

D

L

L

P

P

G

A

S

T

L

L

G

A

V

E

A

A

L

L

T

E

E

E

M

F

E

K

N

S

S

N

E

E

L

V

V

M

A

V

E

K

A

A

L

L

G

G

E

E

H

H

V

L

F

F

D

E

F

H

F

F

L

I

R

E

N

A

K

K

R

E

S

I

E

E

W

W

E

D

N

M

Y

F

R

R

S

T

H

Q

V

V

T

H

P

P

F

W

E

E

L

R

K

E

N

Q

Y

Y

L

M

S

S

active site: D52 (= D52), E131 (= E132), E133 (= E134), E188 (= E189), E195 (= E196), H244 (= H245), R315 (= R316), E332 (= E336), R334 (= R338)
binding adenosine-5'-diphosphate: E131 (= E132), E183 (= E184), D197 (= D198), Y200 (= Y201), N246 (≠ H247), S248 (= S249), R320 (= R321), R330 (= R334)
binding magnesium ion: E131 (= E132), E131 (= E132), E133 (= E134), E188 (= E189), E195 (= E196), E195 (= E196), H244 (= H245), E332 (= E336)
binding l-methionine-s-sulfoximine phosphate: E133 (= E134), E188 (= E189), H244 (= H245), R297 (= R298), E303 (= E304), R315 (= R316), R334 (= R338)

P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 5 papers)
46% identity, 99% coverage: 6:445/446 of query aligns to 7:442/444 of P12425

query
sites
P12425

E

E

F

D

V

I

L

E

R

K

T

L

L

V

E

K

E

E

R

E

D

N

I

V

R

K

F

Y

V

I

R

R

L

L

W

Q

F

F

T

T

D

D

V

I

L

L

G

G

Y

T

L

I

K

K

S

N

V

V

A

E

I

I

A

P

P

V

A

S

E

Q

L

L

E

G

G

K

A

A

F

L

E

D

E

N

G

K

I

V

G

M

F

F

D

D

G

G

S

S

I

I

E

E

G
|
G

F

F

A

V

R
|
R

V

I

S

E

E

E

A

S

D

D

M

M

V

Y

A

L

R

Y

P

P

D

D

P

L

S

N

T

T

F

F

Q

V

V

I

L

F

P

P

W

W

T

T

T

A

S

E

A

K

G

G

N

K

H

-

Y

-

S

V

A

A

R

R

M

F

F

I

C

C

D

D

I

I

T

Y

M

N

P

P

D

D

G

G

S

T

P

P

S

F

W

E

A

G

D

D

S

P

R

R

H

N

V

N

L

L

R

K

R

R

Q

I

L

L

S

K

K

E

A

M

S

E

D

D

L

L

G

G

F

F

S

S

C

D

Y

F

-

N

V

L

H

G

P

P

E
|
E

I

P

E
|
E

F

F

L

L

L

F

K

K

P

L

G

-

P

-

D

D

D

E

G

K

T

G

Q

E

P

P

I

T

P

L

A

E

-

L

-

N

D

D

N

K

G

G

Y

Y

F

F

D

D

Q

L

A

A

V

P

H

T

D

D

A

L

A

G

P

E

N

N

F

C

R

R

H

D

A

I

I

V

D

L

A

E

L

L

E

E

Q

E

M

M

G

G

I

F

S

E

V

I

E

E

F

A

S

S

H

H

E
|
E

G
x
V

A

A

P

P

G

G

Q

Q

Q

H

E
|
E

I

I

D

D

L

F

R

K

Y

Y

A

A

D

G

A

A

L

V

S

R

M

S

A

C

D

D

N

D

V

I

M

Q

T

T

F

F

R

K

Y

L

L

V

V

V

K

K

E

T

V

I

A

A

L

R

A

K

D

H

G

G

V

L

R

H

A

A

S

T

F

F

M

M

P

P

K

K

P

P

F

L

A

F

E

G

H

V

P

N

G
|
G

S

S

A

G

M

M

H
|
H

T

C

H

N

M

L

S

S

L

L

F

F

E

K

G

N

D

G

T

V

N

N

A

A

F

F

H

F

S

D

P

E

D

N

D

A

P

D

L

L

Q

Q

L

L

S

S

D

E

V

T

G

A

K

K

S

H

F

F

I

I

A

A

G

G

I

I

L

V

E

K

H

H

A

A

N

T

E

S

I

F

S

T

A

A

V

V

T

T

N

N

Q

P

W

T

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

H

P

G
|
G

G

Y

E
|
E

A

A

P
|
P

T

C

A

Y

A

V

S

A

W

W

G

S

A

A

A

Q

N

N

R

R

S

S

A

P

L

L

V

I

R

R

V

I

P

P

M

-

Y

-

T

-

P

A

H

S

K

R

V

G

S

I

S

S

R

T

R

R

V

V

E
|
E

V

V

R

R

S

S

P

V

D

D

S

P

A

A

C

A

N

N

P

P

Y

Y

L

L

T

A

F

L

A

S

V

V

L

L

A

A

G

G

L

L

R

D

G

G

I

I

E

-

K

K

N

N

Y

K

V

L

L

E

G

A

P

P

Q

A

A

P

E

I

D

D

-

R

N

N

V

I

W

Y

S

V

L

M

T

S

P

K

E

E

R

R

R

M

A

E

M

N

G

G

Y

I

K

V

E

D

L

L

P

P

G

A

S

T

L

L

G

A

V

E

A

A

L

L

T

E

E

E

M

F

E

K

N

S

S

N

E

E

L

V

V

M

A

V

E

K

A

A

L

L

G

G

E

E

H

H

V

L

F

F

D

E

F

H

F

F

L

I

R

E

N

A

K

K

R

E

S

I

E
|
E

W

W

E

D

N

M

Y

F

R

R

S

T

H

Q

V

V

T

H

P

P

F

W

E

E

L

R

K

E

N

Q

Y

Y

L

M

S

S

G59 (= G58) mutation to R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
R62 (= R61) Important for inhibition by glutamine; mutation to A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.
E132 (= E132) binding Mg(2+)
E134 (= E134) binding Mg(2+)
E189 (= E189) binding Mg(2+)
V190 (≠ G190) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E196) binding Mg(2+)
G241 (= G241) binding L-glutamate
H245 (= H245) binding Mg(2+)
G302 (= G302) mutation to E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
E304 (= E304) mutation to A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
P306 (= P306) mutation to H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
E333 (= E336) binding Mg(2+)
E424 (= E427) mutation to K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4s0rD Structure of gs-tnra complex (see paper)
46% identity, 99% coverage: 6:445/446 of query aligns to 10:445/447 of 4s0rD

query
sites
4s0rD

E

E

F

D

V

I

L

E

R

K

T

L

L

V

E

K

E

E

R

E

D

N

I

V

R

K

F

Y

V

I

R

R

L

L

W

Q

F

F

T

T

D

D

V

I

L

L

G

G

Y

T

L

I

K

K

S

N

V

V

A

E

I

I

A

P

P

V

A

S

E

Q

L

L

E

G

G

K

A

A

F

L

E

D

E

N

G

K

I

V

G

M

F

F

D
|
D

G

G

S

S

I

I

E

E

G

G

F
|
F

A
x
V

R
|
R

V
x
I

S

E

E

E

A

S

D

D

M

M

V

Y

A

L

R

Y

P

P

D

D

P

L

S

N

T

T

F

F

Q

V

V

I

L

F

P

P

W

W

T

T

T

A

S

E

A

K

G

G

N

K

H

-

Y

-

S

V

A

A

R

R

M

F

F

I

C

C

D

D

I

I

T

Y

M

N

P

P

D

D

G

G

S

T

P

P

S

F

W

E

A

G

D

D

S

P

R

R

H

N

V

N

L

L

R

K

R

R

Q

I

L

L

S

K

K

E

A

M

S

E

D

D

L

L

G

G

F

F

S

S

C

D

Y

F

-

N

V

L

H

G

P

P

E
|
E

I

P

E
|
E

F

F

L

L

L

F

K

K

P

L

G

-

P

-

D

D

D

E

G

K

T

G

Q

E

P

P

I

T

P

L

A

E

-

L

-

N

D

D

N

K

G

G

Y

Y

F

F

D
|
D

Q

L

A

A

V

P

H

T

D

D

A

L

A

G

P

E

N

N

F

C

R

R

H

D

A

I

I

V

D

L

A

E

L

L

E

E

Q

E

M

M

G

G

I

F

S

E

V

I

E

E

F

A

S

S

H

H

E
|
E

G

V

A

A

P

P

G

G

Q

Q

Q

H

E
|
E

I

I

D

D

L

F

R

K

Y

Y

A

A

D

G

A

A

L

V

S

R

M

S

A

C

D

D

N

D

V

I

M

Q

T

T

F

F

R

K

Y

L

L

V

V

V

K

K

E

T

V

I

A

A

L

R

A

K

D

H

G

G

V

L

R

H

A

A

S

T

F

F

M

M

P

P

K

K

P

P

F

L

A

F

E
x
G

H
x
V

P
x
N

G

G

S

S

A

G

M

M

H
|
H

T

C

H

N

M

L

S

S

L

L

F

F

E

K

G

N

D

G

T

V

N

N

A

A

F

F

H

F

S

D

P

E

D

N

D

A

P

D

L

L

Q

Q

L

L

S

S

D

E

V

T

G

A

K

K

S

H

F

F

I

I

A

A

G

G

I

I

L

V

E

K

H

H

A

A

N

T

E

S

I

F

S

T

A

A

V

V

T

T

N

N

Q

P

W

T

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

H

P

G
|
G

G
x
Y

E
|
E

A

A

P

P

T

C

A

Y

A

V

S

A

W

W

G

S

A

A

A

Q

N

N

R
|
R

S

S

A

P

L

L

V

I

R

R

V

I

P

P

M

-

Y

-

T

-

P

A

H

S

K

R

V

G

S

I

S

S

R

T

R

R

V

V

E
|
E

V

V

R
|
R

S

S

P

V

D

D

S

P

A

A

C

A

N

N

P

P

Y

Y

L

L

T

A

F

L

A

S

V

V

L

L

A

A

G

G

L

L

R

D

G

G

I

I

E

-

K

K

N

N

Y

K

V

L

L

E

G

A

P

P

Q

A

A

P

E

I

D

D

-

R

N

N

V

I

W
x
Y

S

V

L

M

T

S

P

K

E

E

R

R

R

M

A

E

M

N

G

G

Y

I

K

V

E

D

L

L

P

P

G

A

S

T

L

L

G

A

V

E

A

A

L

L

T

E

E

E

M

F

E

K

N

S

S

N

E

E

L

V

V

M

A

V

E

K

A

A

L

L

G

G

E

E

H

H

V

L

F

F

D

E

F
x
H

F

F

L

I

R

E

N

A

K

K

R

E

S
x
I

E

E

W

W

E

D

N
x
M

Y

F

R

R

S

T

H

Q

V

V

T

H

P

P

F

W

E

E

L

R

K

E

N

Q

Y

Y

L

M

S

S

active site: D56 (= D52), E135 (= E132), E137 (= E134), E192 (= E189), E199 (= E196), H248 (= H245), R319 (= R316), E336 (= E336), R338 (= R338)
binding glutamine: E137 (= E134), E192 (= E189), R301 (= R298), E307 (= E304)
binding magnesium ion: I66 (≠ V62), E135 (= E132), E135 (= E132), E199 (= E196), H248 (= H245), H248 (= H245), E336 (= E336), H419 (≠ F419)
binding : F63 (= F59), V64 (≠ A60), R65 (= R61), I66 (≠ V62), D161 (= D158), G241 (≠ E238), V242 (≠ H239), N243 (≠ P240), G305 (= G302), Y306 (≠ G303), Y376 (≠ W376), I426 (≠ S426), M430 (≠ N430)

7tdpA Structure of paenibacillus polymyxa gs bound to met-sox-p-adp (transition state complex) to 1.98 angstom (see paper)
45% identity, 99% coverage: 6:446/446 of query aligns to 5:438/439 of 7tdpA

query
sites
7tdpA

E

E

F

D

V

I

L

I

R

R

T

I

L

A

E

E

R

E

D

N

I

V

R

R

F

F

V

I

R

R

L

L

W

Q

F

F

T

T

D

D

V

L

L

L

G

G

Y

T

L

I

K

K

S

N

V

V

A

E

I

I

A

P

P

V

A

S

E

Q

L

L

E

E

G

K

A

A

F

L

E

D

E

N

G

K

I

M

G

M

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F

Y

A

V

R

R

V

I

S

E

E

E

A

S

D

D

M

M

V

Y

A

L

R

Y

P

P

D

D

P

L

S

D

T

T

F

W

Q

V

V

V

L

F

P

P

W

W

T

S

T

D

S

R

A

V

G

-

N

-

H

-

Y

-

S

-

A

A

R

R

M

L

F

I

C

C

D

D

I

I

T

Y

M

K

P

P

D

D

G

G

S

S

P

P

S

F

W

A

A

G

D

D

S

P

R

R

H

G

V

I

L

L

R

K

R

R

Q

V

L

L

S

K

K

E

A

A

S

E

D

E

L

L

G

G

F

Y

-

T

S

S

C

M

Y
x
N

V

V

H
x
G

P

P

E
|
E

I

P

E
|
E

F

F

L

L

L

F

K

K

P

T

G

D

P

E

D

K

D

G

G

D

T

P

Q

T

P

T

I

E

P

L

A

N

D

D

N

Q

G

G

Y

Y

F

F

D

D

Q

L

A

A

V

P

H

M

D

D

A

L

A

G

P

E

N

N

F

C

R

R

H

E

A

I

I

V

D

L

A

K

L

L

E

E

Q

E

M

M

G

G

I

F

S

E

V

I

E
|
E

F

A

S

S

H

H

E
|
E

G

V

A

A

P

P

G

G

Q

Q

Q

H

E
|
E

I

I

D
|
D

L

F

R

K

Y
|
Y

A

A

D

D

A

A

L

V

S

K

M

A

A

A

D

D

N

Q

V

I

M

Q

T

T

F

F

R

K

Y

L

L

V

V

V

K

K

E

T

V

I

A

A

L

R

A

Q

D

H

G

G

V

L

R

H

A

A

S

T

F

F

M

M

P

P

K

K

P

P

F

L

A

F

E

G

H

V

P

N

G
|
G

S

S

A

G

M

M

H
|
H

T

C

H
x
N

M

Q

S
|
S

L

L

F

F

E

K

G

D

D

N

T

E

N

N

A

V

F

F

H

Y

S

D

P

E

D

T

D

D

P

E

L

L

Q

G

L

L

S

S

D

Q

V

T

G

A

K

R

S

H

F

Y

I

M

A

A

G

G

I

I

L

L

E

K

H

H

A

A

N

R

E

A

I

M

S

A

A

A

V

I

T

T

N

N

Q

P

W

T

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

H

P

G

G

G

Y

E
|
E

A

A

P

P

T

C

A

Y

A

V

S

A

W

W

G

S

A

A

A

S

N

N

R
|
R

S

S

A

P

L

M

V

I

R
|
R

V

I

P

P

M

-

Y

-

T

-

P

A

H

S

K

R

V

G

S

L

S

S

R

T

R
|
R

V

V

E
|
E

V

V

R
|
R

S

N

P

P

D

D

S

P

A

A

C

A

N

N

P

P

Y

Y

L

L

T

A

F

L

A

A

V

V

L

M

L

L

A

R

A

A

G

G

L

L

R

D

G

G

I

I

E

K

K

R

N

Q

Y

M

V

A

L

L

G

P

P

A

Q

P

A

I

E

D

D

R

N

N

V

I

W

Y

S

V

L

M

T

S

P

E

E

E

R

R

R

I

A

E

M

E

G

G

Y

I

K

P

E

S

L

L

P

P

G

A

S

D

L

L

G

K

V

E

A

A

L

L

T

S

E

E

M

L

E

I

N

R

S

S

E

E

L

V

V

I

A

S

E

D

A

A

L

L

G

G

E

D

H

H

V

A

F

L

D

A

F

Y

F

F

L

Y

R

E

N

L

K

K

R

E

S

I

E

E

W

W

E

D

N

M

Y

Y

R

R

S

T

H

Q

V

V

T

H

P

Q

F

W

E

E

L

R

K

D

N

Q

Y

Y

L

L

S

T

L

L

binding adenosine-5'-diphosphate: N123 (≠ Y128), G125 (≠ H130), E127 (= E132), E179 (= E184), D193 (= D198), Y196 (= Y201), N242 (≠ H247), S244 (= S249), R316 (= R321), R326 (= R334)
binding magnesium ion: E127 (= E132), E127 (= E132), E129 (= E134), E184 (= E189), E191 (= E196), E191 (= E196), H240 (= H245), E328 (= E336)
binding l-methionine-s-sulfoximine phosphate: E127 (= E132), E129 (= E134), E184 (= E189), E191 (= E196), G236 (= G241), H240 (= H245), R293 (= R298), E299 (= E304), R311 (= R316), R330 (= R338)

8oooA Glutamine synthetase from methanothermococcus thermolithotrophicus in complex with 2-oxoglutarate and mgatp at 2.15 a resolution (see paper)
43% identity, 99% coverage: 5:446/446 of query aligns to 3:447/447 of 8oooA

query
sites
8oooA

M

V

E

E

F

Q

V

V

L

L

R

E

T

Y

L

V

E

K

E

S

R

N

D

N

I

V

R

K

F
|
F

V

M

R
|
R

L

F

W

Q

F

F

T

V

D

D

V

I

L

L

G

G

Y

V

L

P

K

K

S

N

V

V

A
|
A

I

F

-

P

-

I

-

K

-

A

-

G

-

E

-

K

A

G

P

I

A

E

E

E

L

L

E

R

G

D

A

V

F

L

E

E

E

N

G

G

I

L

G

Y

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F

F

A

V

R

G

V

I

S

N

E

E

A

S

D

D

M

M

V

M

A

L

R

K

P

P

D

D

P

L

S

S

T

T

F

F

Q

S

V

V

L

L

P

P

W

W

T
x
R

T

P

S

S

A

E

G

K

N

S

H

-

Y

-

S
x
V

A

A

R

R

M

V

F

I

C

C

D

D

I

V

T

Y

M

T

P

T

D

K

G

G

S

K

P

P

S

F

W

E

A

G

D

D

S

P

R

R

H

G

V

C

L

L

R

K

R

R

Q

V

L

M

S

E

K

E

-

F

A

K

S

K

D

E

L

F

G

N

F

G

S

E

C

Y

Y

F

V

V

H

G

P

P

E
|
E

I

P

E

E

F

F

L

L

K

K

P

K

G

D

P

P

D

H

D

N

G

P

T

H

Q

K

P

Y

I

I

P

P

A

A

D

D

N

D

G

G

Y

Y

F

F

D

D

Q

L

A

E

V

P

H

M

D

D

A

E

A

A

P
|
P

N

D

F

I

R
|
R

H

D

A

I

I

V

D

F

A

A

L

L

E

E

Q

N

M

L

G

G

I

F

S

H

V

V

E
|
E

F

A

S
|
S

H

H

E

E

G

V

A

A

P

P

G

G

Q

Q

Q

H

E

E

I

V

D

D

L
x
F

R
x
K

Y
x
F

A

D

D

D

A

A

L

L

S

K

M

T

A

A

D

D

N

S

V

V

M

I

T

T

F

F

R

K

Y

T

L

T

V

I

K

K

E

T

V

I

A

A

L

E

A

Q

D

H

G

G

V

L

R

K

A

A

S

T

F

F

M

M

P

P

K

K

P

P

F

F

A

F

E

G

H

M

P

N

G

G

S

S

A

G

M

M

H

H

T

C

H
|
H

M

Q

S
|
S

L

I

F

W

E

L

G

N

D

G

T

E

N

P

A

S

F

F

H

Y

S

D

P

E

D

N

D

A

P

P

L

Y

Q

Q

L

L

S

S

D

E

V

T

G

C

K

M

S

N

F

Y

I

V

A

A

G

G

I

I

L

L

E

K

H

H

A

A

N

K

E

A

I

I

S

V

A

A

V

I

T

T

N

N

Q

P

W

T

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

H

P

G

G

G

Y

E

E

A

A

P

P

T

V

A

N

A

I

S

A

W

W

G

A

A

N

A

S

N

N

R

R

S

S

A

A

L

I

V

I

R
|
R

V

V

P

P

M

A

Y

-

T

-

P

-

H

A

K

R

V

G

S

K

S

G

R

T

R
|
R

V

I

E

E

V

F

R

R

S

A

P

P

D

D

S

P

A

S

C

C

N

N

P

P

Y

Y

L

L

T

A

F

F

A

T

V

V

L

M

L

L

A

A

G

G

L

L

R

D

G

G

I

V

E

-

K

K

N

N

Y

K

V

L

L

D

G

A

P

P

Q

E

-

P

A

V

E

E

D

R

N

N

V

I

W

F

S

A

L

M

T

S

P

E

E

A

E

E

R

K

A

E

M

L

G

G

Y

I

K

E

E

S

L

V

P

P

G

A

S

N

L

L

G

K

V

A

A

A

L

L

T

D

E

E

M

L

E

E

N

N

S

N

E

D

L

V

V

L

A

K

E

N

A

A

L

L

G

G

E

K

H

H

V

I

F

F

D

E

F

S

F

F

L

L

R

E

N

I

K

K

R

N

S

A

E

E

W

W

E

D

N

S

Y

F

R

R

S

T

H

S

V

V

T

T

P

D

F

W

E

E

L

T

K

T

N

A

Y

Y

L

L

S

K

L

I

binding 2-oxoglutaric acid: F17 (= F19), R19 (= R21), A33 (= A35), R87 (≠ T82), V93 (≠ S90), P170 (= P166), R173 (= R169), R174 (= R170), S190 (= S186)
binding adenosine-5'-triphosphate: E136 (= E132), E188 (= E184), F203 (≠ L199), K204 (≠ R200), F205 (≠ Y201), H251 (= H247), S253 (= S249), R325 (= R321), R335 (= R334)

8ooqB Glutamine synthetase from Methanothermococcus thermolithotrophicus (see paper)
43% identity, 99% coverage: 5:446/446 of query aligns to 2:446/446 of 8ooqB

query
sites
8ooqB

M

V

E

E

F

Q

V

V

L

L

R

E

T

Y

L

V

E

K

E

S

R

N

D

N

I

V

R

K

F
|
F

V

M

R
|
R

L

F

W

Q

F

F

T

V

D

D

V

I

L

L

G

G

Y

V

L

P

K

K

S

N

V

V

A
|
A

I

F

-

P

-

I

-

K

-

A

-

G

-

E

-

K

A

G

P

I

A

E

E

E

L

L

E

R

G

D

A

V

F

L

E

E

E

N

G

G

I

L

G

Y

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F

F

A

V

R

G

V

I

S

N

E

E

A

S

D

D

M

M

V

M

A

L

R

K

P

P

D

D

P

L

S

S

T

T

F

F

Q

S

V

V

L

L

P

P

W

W

T
x
R

T

P

S

S

A

E

G

K

N

S

H

-

Y

-

S
x
V

A

A

R

R

M

V

F

I

C

C

D

D

I

V

T

Y

M

T

P

T

D

K

G

G

S

K

P

P

S

F

W

E

A

G

D

D

S

P

R

R

H

G

V

C

L

L

R

K

R

R

Q

V

L

M

S

E

K

E

-

F

A

K

S

K

D

E

L

F

G

N

F

G

S

E

C

Y

Y

F

V

V

H

G

P

P

E

E

I

P

E
|
E

F

F

L

L

K

K

P

K

G

D

P

P

D

H

D

N

G

P

T

H

Q

K

P

Y

I

I

P

P

A

A

D

D

N

D

G

G

Y

Y

F

F

D

D

Q

L

A

E

V

P

H

M

D

D

A

E

A

A

P
|
P

N

D

F

I

R
|
R

H

D

A

I

I

V

D

F

A

A

L

L

E

E

Q

N

M

L

G

G

I

F

S

H

V

V

E

E

F

A

S
|
S

H

H

E
|
E

G

V

A

A

P

P

G

G

Q

Q

Q

H

E
|
E

I

V

D

D

L

F

R

K

Y

F

A

D

D

D

A

A

L

L

S

K

M

T

A

A

D

D

N

S

V

V

M

I

T

T

F

F

R

K

Y

T

L

T

V

I

K

K

E

T

V

I

A

A

L

E

A

Q

D

H

G

G

V

L

R

K

A

A

S

T

F

F

M

M

P

P

K

K

P

P

F

F

A

F

E

G

H

M

P

N

G

G

S

S

A

G

M

M

H

H

T

C

H

H

M

Q

S

S

L

I

F

W

E

L

G

N

D

G

T

E

N

P

A

S

F

F

H

Y

S

D

P

E

D

N

D

A

P

P

L

Y

Q

Q

L

L

S

S

D

E

V

T

G

C

K

M

S

N

F

Y

I

V

A

A

G

G

I

I

L

L

E

K

H

H

A

A

N

K

E

A

I

I

S

V

A

A

V

I

T

T

N

N

Q

P

W

T

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

H

P

G

G

G

Y

E

E

A

A

P

P

T

V

A

N

A

I

S

A

W

W

G

A

A

N

A

S

N

N

R

R

S

S

A

A

L

I

V

I

R

R

V

V

P

P

M

A

Y

-

T

-

P

-

H

A

K

R

V

G

S

K

S

G

R

T

R

R

V

I

E

E

V

F

R

R

S

A

P

P

D

D

S

P

A

S

C

C

N

N

P

P

Y

Y

L

L

T

A

F

F

A

T

V

V

L

M

L

L

A

A

G

G

L

L

R

D

G

G

I

V

E

-

K

K

N

N

Y

K

V

L

L

D

G

A

P

P

Q

E

-

P

A

V

E

E

D

R

N

N

V

I

W

F

S

A

L

M

T

S

P

E

E

A

E

E

R

K

A

E

M

L

G

G

Y

I

K

E

E

S

L

V

P

P

G

A

S

N

L

L

G

K

V

A

A

A

L

L

T

D

E

E

M

L

E

E

N

N

S

N

E

D

L

V

V

L

A

K

E

N

A

A

L

L

G

G

E

K

H

H

V

I

F

F

D

E

F

S

F

F

L

L

R

E

N

I

K

K

R

N

S

A

E

E

W

W

E

D

N

S

Y

F

R

R

S

T

H

S

V

V

T

T

P

D

F

W

E

E

L

T

K

T

N

A

Y

Y

L

L

S

K

L

I

binding 2-oxoglutaric acid: F16 (= F19), R18 (= R21), A32 (= A35), R86 (≠ T82), V92 (≠ S90), P169 (= P166), R172 (= R169), R173 (= R170), S189 (= S186)
binding magnesium ion: E137 (= E134), E192 (= E189), E199 (= E196)

7tf9S L. Monocytogenes gs(14)-q-glnr peptide (see paper)
45% identity, 99% coverage: 6:446/446 of query aligns to 6:442/443 of 7tf9S

query
sites
7tf9S

E

E

F

D

V

I

L

F

R

R

T

F

L

A

E

D

E

E

R

Q

D

N

I

V

R

K

F

F

V

I

R

R

L

L

W

Q

F

F

T

T

D

D

V

I

L

L

G

G

Y

I

L

I

K

K

S

N

V

V

A

E

I

I

A

P

P

V

A

S

E

Q

L

L

E

K

G

K

A

A

F

L

E

D

E

N

G

K

I

I

G

M

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F
|
F

A
x
V

R

R

V

I

S

E

E

E

A

S

D

D

M

M

V

Y

A

L

R

F

P

P

D

D

P

L

S

D

T

T

F

W

Q

V

V

V

L

F

P

P

W

W

T

T

T

A

S

E

A

K

G

G

N

K

H

-

Y

-

S

V

A

A

R

R

M

M

F

I

C

C

D

D

I

I

T

Y

M

N

P

P

D

D

G

M

S

T

P

P

S

F

W

A

A

G

D

D

S

P

R

R

H

A

V

N

L

L

R

K

R

R

Q

V

L

L

S

K

K

E

A

M

S

E

D

E

L

L

G

G

F

F

S

T

C

E

Y

F

-

N

V

L

H

G

P

P

E
|
E

I

P

E
|
E

F

F

L

L

L

F

K

K

P

L

G

-

P

-

D

D

D

E

G

N

T

R

Q

R

P

P

I

T

P

L

A

E

-

L

-

N

D

D

N

S

G

G

Y
|
Y

F

F

D

D

Q

L

A

A

V

P

H

T

D

D

A

L

A

G

P

E

N

N

F

C

R

R

H

D

A

I

I

V

D

L

A

E

L

L

E

E

Q

E

M

M

G

G

I

F

S

E

V

I

E

E

F

A

S

S

H

H

E
|
E

G

V

A

A

P

P

G

G

Q

Q

Q

H

E
|
E

I

I

D

D

L

F

R

K

Y

Y

A

E

D

D

A

A

L

I

S

T

M

A

A

C

D

D

N

S

V

I

M

Q

T

T

F

F

R

K

Y

L

L

V

V

V

K

K

E

T

V

I

A

A

L

R

A

K

D

H

G

G

V

L

R

H

A

A

S

T

F

F

M

M

P

P

K

K

P

P

F

L

A

F

E

G

H

V

P

N

G
|
G

S

S

A
x
G

M

M

H
|
H

T

F

H

N

M

M

S

S

L

L

F

F

E

N

G

E

D

K

T

G

N

N

A

A

F

F

H

F

S

D

P

E

D

S

D

G

P

E

L

L

Q

E

L

L

S

S

D

Q

V

T

G

A

K

Y

S

H

F

F

I

L

A

A

G

G

I

M

L

L

E

K

H

H

A

A

N

R

E

G

I

Y

S

T

A

A

V

V

T

T

N

N

Q

P

W

T

V

I

N

N

S

S

Y

F

K

K

R
|
R

L

L

V

V

H

P

G

G

G

Y

E
|
E

A

A

P

P

T

C

A

Y

A

I

S

A

W

W

G

S

A

G

A

K

N

N

R

R

S

S

A

P

L

L

V

V

R

R

V

V

P

P

M

-

Y

-

T

-

P

S

H

S

K

R

V

G

S

L

S

S

R

T

R

R

V

L

E
|
E

V

L

R

R

S

S

P

V

D

D

S

P

A

S

C

A

N

N

P

P

Y

Y

L

L

T

A

F

M

A

A

V

V

L

L

A

K

A

A

G

G

L

L

R

S

G

G

I

I

E

K

K

D

N

E

Y

L

V

T

L

P

G

P

P

A

Q

P

A

V

E

D

D

R

N

N

V

I

W

Y

S

G

L

M

T

N

P

E

E

E

R

R

R

E

A

A

M

T

G

G

Y

I

K

Y

E

D

L

L

P

P

G

E

S

S

L

L

G

G

V

H

A

A

L

L

T

I

E

E

M

L

E

E

N

K

S

N

E

E

L

I

V

I

A

K

E

D

A

G

L

L

G

G

E

E

H

H

V

I

F

F

D

E

F

H

F

F

L

I

R
x
E

N

A

K

K

R

T

S
x
I

E

E

W

C

E

D

N
x
M

Y

F

R

R

S

T

H

A

V

V

T

H

P

P

F

W

E

E

L

R

K

E

N

Q

Y

Y

L

L

S

E

L

I

binding glutamine: E133 (= E134), Y155 (= Y156), E188 (= E189), G240 (= G241), G242 (≠ A243), R297 (= R298), E303 (= E304)
binding magnesium ion: E131 (= E132), E133 (= E134), E188 (= E189), E195 (= E196), H244 (= H245), E332 (= E336)
binding : F59 (= F59), V60 (≠ A60), E418 (≠ R422), I422 (≠ S426), M426 (≠ N430)

7tenA Glutamine synthetase (see paper)
45% identity, 99% coverage: 6:446/446 of query aligns to 5:441/442 of 7tenA

query
sites
7tenA

E

E

F

D

V

I

L

F

R

R

T

F

L

A

E

D

E

E

R

Q

D

N

I

V

R

K

F

F

V

I

R

R

L

L

W

Q

F

F

T

T

D

D

V

I

L

L

G

G

Y

I

L

I

K

K

S

N

V

V

A

E

I

I

A

P

P

V

A

S

E

Q

L

L

E

K

G

K

A

A

F

L

E

D

E

N

G

K

I

I

G

M

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F

F

A

V

R

R

V

I

S

E

E

E

A

S

D

D

M

M

V

Y

A

L

R

F

P

P

D

D

P

L

S

D

T

T

F

W

Q

V

V

V

L

F

P

P

W

W

T

T

T

A

S

E

A

K

G

G

N

K

H

-

Y

-

S

V

A

A

R

R

M

M

F

I

C

C

D

D

I

I

T

Y

M

N

P

P

D

D

G

M

S

T

P

P

S

F

W

A

A

G

D

D

S

P

R

R

H

A

V

N

L

L

R

K

R

R

Q

V

L

L

S

K

K

E

A

M

S

E

D

E

L

L

G

G

F

F

S

T

C

E

Y

F

-

N

V

L

H
x
G

P

P

E
|
E

I

P

E
|
E

F

F

L

L

L

F

K

K

P

L

G

-

P

-

D

D

D

E

G

N

T

R

Q

R

P

P

I

T

P

L

A

E

-

L

-

N

D

D

N

S

G

G

Y

Y

F

F

D

D

Q

L

A

A

V

P

H

T

D

D

A

L

A

G

P

E

N

N

F

C

R

R

H

D

A

I

I

V

D

L

A

E

L

L

E

E

Q

E

M

M

G

G

I

F

S

E

V

I

E
|
E

F

A

S

S

H

H

E
|
E

G

V

A

A

P

P

G

G

Q

Q

Q

H

E
|
E

I

I

D
|
D

L
x
F

R
x
K

Y
|
Y

A

E

D

D

A

A

L

I

S

T

M

A

A

C

D

D

N

S

V

I

M

Q

T

T

F

F

R

K

Y

L

L

V

V

V

K

K

E

T

V

I

A

A

L

R

A

K

D

H

G

G

V

L

R

H

A

A

S

T

F

F

M

M

P

P

K

K

P

P

F

L

A

F

E

G

H

V

P
x
N

G
|
G

S

S

A

G

M

M

H
|
H

T

F

H
x
N

M

M

S
|
S

L

L

F

F

E

N

G

E

D

K

T

G

N

N

A

A

F

F

H

F

S

D

P

E

D

S

D

G

P

E

L

L

Q

E

L

L

S

S

D

Q

V

T

G

A

K

Y

S

H

F

F

I

L

A

A

G

G

I

M

L

L

E

K

H

H

A

A

N

R

E

G

I

Y

S

T

A

A

V

V

T

T

N

N

Q

P

W

T

V

I

N

N

S

S

Y

F

K

K

R
|
R

L

L

V

V

H

P

G

G

G

Y

E
|
E

A

A

P

P

T

C

A

Y

A

I

S

A

W

W

G

S

A

G

A

K

N

N

R
|
R

S

S

A

P

L

L

V

V

R
|
R

V

V

P

P

M

-

Y

-

T

-

P

S

H

S

K

R

V

G

S

L

S
|
S

R

T

R
|
R

V

L

E

E

V

L

R
|
R

S

S

P

V

D

D

S

P

A

S

C

A

N

N

P

P

Y

Y

L

L

T

A

F

M

A

A

V

V

L

L

A

K

A

A

G

G

L

L

R

S

G

G

I

I

E

K

K

D

N

E

Y

L

V

T

L

P

G

P

P

A

Q

P

A

V

E

D

D

R

N

N

V

I

W

Y

S

G

L

M

T

N

P

E

E

E

R

R

R

E

A

A

M

T

G

G

Y

I

K

Y

E

D

L

L

P

P

G

E

S

S

L

L

G

G

V

H

A

A

L

L

T

I

E

E

M

L

E

E

N

K

S

N

E

E

L

I

V

I

A

K

E

D

A

G

L

L

G

G

E

E

H

H

V

I

F

F

D

E

F

H

F

F

L

I

R

E

N

A

K

K

R

T

S

I

E

E

W

C

E

D

N

M

Y

F

R

R

S

T

H

A

V

V

T

H

P

P

F

W

E

E

L

R

K

E

N

Q

Y

Y

L

L

S

E

L

I

binding adenosine-5'-diphosphate: G128 (≠ H130), E130 (= E132), E182 (= E184), D196 (= D198), F197 (≠ L199), K198 (≠ R200), Y199 (= Y201), N245 (≠ H247), S247 (= S249), R319 (= R321), S327 (= S332), R329 (= R334)
binding l-methionine-s-sulfoximine phosphate: E130 (= E132), E132 (= E134), E187 (= E189), E194 (= E196), N238 (≠ P240), G239 (= G241), H243 (= H245), R296 (= R298), E302 (= E304), R314 (= R316), R333 (= R338)

7tdvC Glutamine synthetase (see paper)
43% identity, 99% coverage: 6:445/446 of query aligns to 6:441/443 of 7tdvC

query
sites
7tdvC

E

E

F

D

V

I

L

R

R

K

T

F

L

V

E

E

R

E

D

N

I

V

R

R

F

Y

V

L

R

R

L

L

W

Q

F

F

T

T

D

D

V

I

L

L

G

G

Y

T

L

I

K

K

S

N

V

V

A

E

I

V

A

P

P

V

A

S

E

Q

L

L

E

E

G

K

A

V

F

L

E

D

E

N

G

E

I

M

G

M

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F

F

A

V

R

R

V

I

S

E

E

E

A

S

D

D

M

M

V

Y

A

L

R

H

P

P

D

D

P

L

S

D

T

T

F

W

Q

V

V

I

L

F

P

P

W

W

T

T

T

-

S

-

A

A

G

G

N

Q

H

G

Y

K

S

V

A

A

R

R

M

L

F

I

C

C

D

D

I

V

T

Y

M

K

P

T

D

D

G

G

S

T

P

P

S

F

W

E

A

G

D

D

S

P

R

R

H

A

V

N

L

L

R

K

R

R

Q

V

L

L

S

K

K

E

A

M

S

E

D

D

L

L

G

G

F

F

S

T

C

D

Y

F

-

N

V

L

H
x
G

P

P

E
|
E

I

P

E
|
E

F

F

L

L

L

F

K

K

P

L

G

-

P

-

D

D

D

E

G

K

T

G

Q

E

P

P

I

T

P

L

A

E

-

L

-

N

D

D

N

D

G

G

Y

Y

F

F

D

D

Q

L

A

A

V

P

H

T

D

D

A

L

A

G

P

E

N

N

F

C

R

R

H

D

A

I

I

V

D

L

A

E

L

L

E

E

Q

D

M

M

G

G

I

F

S

D

V

I

E
|
E

F

A

S

S

H

H

E
|
E

G

V

A

A

P

P

G

G

Q

Q

Q

H

E
|
E

I

I

D
|
D

L
x
F

R
x
K

Y
|
Y

A

A

D

D

A

A

L

V

S

T

M

A

A

C

D

D

N

N

V

I

M

Q

T

T

F

F

R

K

Y

L

L

V

V

V

K

K

E

T

V

I

A

A

L

R

A

K

D

H

G

N

V

L

R

H

A

A

S

T

F

F

M

M

P

P

K

K

P

P

F

L

A

F

E

G

H

V

P

N

G
|
G

S

S

A

G

M

M

H
|
H

T

F

H
x
N

M
x
V

S
|
S

L

L

F

F

E

K

G

G

D

K

T

E

N

N

A

A

F

F

H

F

S

D

P

P

D

N

D

T

P

E

L

M

Q

G

L

L

S

T

D

E

V

T

G

A

K

Y

S

Q

F

F

I

T

A

A

G

G

I

V

L

L

E

K

H

N

A

A

N

R

E

G

I

F

S

T

A

A

V

V

T

C

N

N

Q

P

W

L

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

H

P

G

G

G

Y

E
|
E

A

A

P

P

T

C

A

Y

A

I

S

A

W

W

G

S

A

G

A

K

N

N

R
|
R

S

S

A

P

L

L

V

I

R
|
R

V

V

P

P

M

-

Y

-

T

-

P

S

H

S

K

R

V

G

S

L

S
|
S

R

T

R
|
R

V

I

E
|
E

V

V

R

R

S

S

P

V

D

D

S

P

A

A

C

A

N

N

P

P

Y

Y

L

M

T

A

F

L

A

A

V

A

L

I

L

L

A

E

A

A

G

G

L

L

R

D

G

G

I

I

E

K

K

N

N

K

Y

L

V

K

L

V

G

P

P

E

Q

P

A

V

E

N

D

Q

N

N

V

I

W

Y

S

E

L

M

T

N

P

R

E

E

R

R

R

E

A

A

M

V

G

G

Y

I

K

Q

E

D

L

L

P

P

G

S

S

T

L

L

G

Y

V

T

A

A

L

L

T

K

E

A

M

M

E

R

N

E

S

N

E

E

L

V

V

I

A

K

E

K

A

A

L

L

G

G

E

N

H

H

V

I

F

Y

D

N

F

Q

F

F

L

I

R

N

N

S

K

K

R

S

S

I

E

E

W

W

E

D

N

Y

Y

Y

R

R

S

T

H

Q

V

V

T

S

P

E

F

W

E

E

L

R

K

D

N

Q

Y

Y

L

M

S

K

binding adenosine-5'-diphosphate: G129 (≠ H130), E131 (= E132), E183 (= E184), D197 (= D198), F198 (≠ L199), K199 (≠ R200), Y200 (= Y201), N246 (≠ H247), V247 (≠ M248), S248 (= S249), R320 (= R321), S328 (= S332), R330 (= R334)
binding magnesium ion: E131 (= E132), E131 (= E132), E133 (= E134), E188 (= E189), E195 (= E196), E195 (= E196), H244 (= H245), E332 (= E336)
binding l-methionine-s-sulfoximine phosphate: E131 (= E132), E133 (= E134), E188 (= E189), E195 (= E196), G240 (= G241), H244 (= H245), R297 (= R298), E303 (= E304), R315 (= R316)

7tf6A Glutamine synthetase (see paper)
42% identity, 99% coverage: 6:445/446 of query aligns to 5:436/438 of 7tf6A

query
sites
7tf6A

E

E

F

D

V

I

L

R

R

K

T

F

L

A

E

E

R

E

D

N

I

V

R

R

F

Y

V

L

R

R

L

L

W

Q

F

F

T

T

D

D

V

I

L

L

G

G

Y

T

L

I

K

K

S

N

V

V

A

E

I

V

A

P

P

V

A

S

E

Q

L

L

E

E

G

K

A

V

F

L

E

D

E

N

G

E

I

M

G

M

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F
|
F

A

V

R
|
R

V

I

S

E

E

E

A

S

D

D

M

M

V

Y

A

L

R

H

P

P

D

D

P

L

S

D

T

T

F

W

Q

V

V

I

L

F

P

P

W

W

T

G

T

K

S

V

A

A

G

-

N

-

H

-

Y

-

S

-

A

-

R

R

M

L

F

I

C

C

D

D

I

V

T

Y

M

K

P

T

D

D

G

G

S

T

P

P

S

F

W

E

A

G

D

D

S

P

R

R

H

A

V

N

L

L

R

K

R

R

Q

V

L

L

S

K

K

E

A

M

S

E

D

D

L

L

G

G

F

F

S

T

C

D

Y

F

-

N

V

L

H

G

P

P

E
|
E

I

P

E
|
E

F

F

L

L

L

F

K

K

P

L

G

-

P

-

D

D

D

E

G

K

T

G

Q

E

P

P

I

T

P

L

A

E

-

L

-

N

D

D

N

D

G

G

Y

Y

F

F

D

D

Q

L

A

A

V

P

H

T

D

D

A

L

A

G

P

E

N

N

F

C

R

R

H

D

A

I

I

V

D

L

A

E

L

L

E

E

Q

D

M

M

G

G

I

F

S

D

V

I

E

E

F

A

S

S

H

H

E
|
E

G

V

A

A

P

P

G

G

Q

Q

Q

H

E
|
E

I

I

D

D

L

F

R

K

Y

Y

A

A

D

D

A

A

L

V

S

T

M

A

A

C

D

D

N

N

V

I

M

Q

T

T

F

F

R

K

Y

L

L

V

V

V

K

K

E

T

V

I

A

A

L

R

A

K

D

H

G

N

V

L

R

H

A

A

S

T

F

F

M

M

P

P

K

K

P

P

F

L

A

F

E
x
G

H

V

P
x
N

G
|
G

S

S

A

G

M

M

H
|
H

T

F

H

N

M

V

S

S

L

L

F

F

E

K

G

G

D

K

T

E

N

N

A

A

F

F

H

F

S

D

P

P

D

N

D

T

P

E

L

M

Q

G

L

L

S

T

D

E

V

T

G

A

K

Y

S

Q

F

F

I

T

A

A

G

G

I

V

L

L

E

K

H

N

A

A

N

R

E

G

I

F

S

T

A

A

V

V

T

C

N

N

Q

P

W

L

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

H

P

G
|
G

G
x
Y

E
|
E

A

A

P

P

T

C

A

Y

A

I

S

A

W

W

G

S

A

G

A

K

N

N

R
|
R

S

S

A

P

L

L

V

I

R

R

V

V

P

P

M

-

Y

-

T

-

P

S

H

S

K

R

V

G

S

L

S

S

R

T

R

R

V

I

E
|
E

V

V

R

R

S

S

P

V

D

D

S

P

A

A

C

A

N

N

P

P

Y

Y

L

M

T

A

F

L

A

A

V

A

L

I

L

L

A

E

A

A

G

G

L

L

R

D

G

G

I

I

E

K

K

N

N

K

Y

L

V

K

L

V

G

P

P

E

Q

P

A

V

E

N

D

Q

N

N

V

I

W
x
Y

S

E

L

M

T

N

P

R

E

E

R

R

R

E

A

A

M

V

G

G

Y

I

K

Q

E

D

L

L

P

P

G

S

S

T

L

L

G

Y

V

T

A

A

L

L

T

K

E

A

M

M

E

R

N

E

S

N

E

E

L

V

V

I

A

K

E

K

A

A

L

L

G

G

E

N

H

H

V

I

F

Y

D

N

F

Q

F

F

L

I

R

N

N

S

K

K

R

S

S

I

E

E

W

W

E

D

N
x
Y

Y

Y

R

R

S

T

H

Q

V

V

T

S

P

E

F

W

E

E

L

R

K

D

N
x
Q

Y

Y

L

M

S

K

binding glutamine: E128 (= E134), E183 (= E189), G235 (= G241), H239 (= H245), R292 (= R298), E298 (= E304)
binding magnesium ion: E126 (= E132), E128 (= E134), E183 (= E189), E190 (= E196), H239 (= H245), E327 (= E336)
binding : F58 (= F59), R60 (= R61), G232 (≠ E238), N234 (≠ P240), G296 (= G302), Y297 (≠ G303), R310 (= R316), Y367 (≠ W376), Y421 (≠ N430), Q433 (≠ N442)

Sites not aligning to the query:

binding : 437

P0A9C5 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Escherichia coli (strain K12) (see 3 papers)
35% identity, 99% coverage: 6:446/446 of query aligns to 4:469/469 of P0A9C5

query
sites
P0A9C5

E

E

F

H

V

V

L

L

R

T

T

M

L

L

E

N

E

E

R

H

D

E

I

V

R

K

F

F

V

V

R

D

L

L

W

R

F

F

T

T

D

D

V

T

L

K

G

G

Y

K

L

E

K

Q

S

H

V

V

A

T

I

I

A

P

P

A

A

H

E

Q

L

V

E

N

G

A

A

E

F

F

-

F

E

E

G

G

I

K

G

M

F

F

D

D

G

G

S

S

I

I

E

G

G

G

F

W

A

K

R

G

V

I

S

N

E

E

A

S

D

D

M

M

V

V

A

L

R

M

P

P

D

D

P

A

S

S

T

T

F

A

Q

V

V

I

L

D

P

P

W

F

T

F

T

A

S

D

A

S

G

-

N

-

H

-

Y

-

S

T

A

L

R

I

M

I

F

R

C

C

D

D

I

I

T

L

M

E

P

P

D

G

G

T

S

L

P

Q

S

G

W

Y

-

D

A

R

D

D

S

P

R

R

H

S

V

I

L

A

R

K

R

R

-

A

-

E

Q

D

L

Y

S

L

K

R

A

S

S

T

D

G

L

I

G

A

F

D

S

T

C

V

Y

L

V

F

H

G

P

P

E

E

I

P

E

E

F

F

L

L

L

F

K

D

-

D

-

I

-

R

-

F

-

G

-

S

-

I

-

S

-

G

-

S

-

H

-

V

-

A

-

I

-

D

-

I

-

E

-

G

-

A

-

W

-

N

-

S

-

T

-

Q

-

Y

P

E

G

G

P

G

D

N

D

K

G

G

T

H

Q

R

P

P

I

-

P

-

A

A

D

V

N

K

G

G

Y

Y

F

F

D

P

Q

V

A

P

V

P

H

V

D

D

A

S

A

A

P

Q

N

D

F

I

R

R

R

S

H

E

A

M

I

C

D

L

A

V

L

M

E

E

Q

Q

M

M

G

G

I

L

S

V

V

V

E

E

F

A

S

H

H

H

E

E

G

V

A

A

P

T

-

A

G

G

Q

Q

Q

N

E

E

I

V

D

A

L

T

R

R

Y

F

A

N

D

T

A

M

L

T

S

K

M

K

A

A

D

D

N

E

V

I

M

Q

T

I

F

Y

R

K

Y

Y

L

V

V

V

K

H

E

N

V

V

A

A

L

H

A

R

D

F

G

G

V

K

R

T

A

A

S

T

F

F

M

M

P

P

K

K

P

P

F

M

A

F

E

G

H

D

P

N

G

G

S

S

A

G

M

M

H

H

T

C

H

H

M

M

S

S

L

L

F

S

E

K

G

N

D

G

T

V

N

N

A

L

F

F

H

-

S

A

P

G

D

D

D

K

P

Y

L

A

Q

G

L

L

S

S

D

E

V

Q

G

A

K

L

S

Y

F

Y

I

I

A

G

G

G

I

V

L

I

E

K

H

H

A

A

N

K

E

A

I

I

S

N

A

A

V

L

T

A

N

N

Q

P

W

T

V

T

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

H

P

G

G

G

Y

E

E

A

A

P

P

T

V

A

M

A

L

S

A

W

Y

G

S

A

A

A

R

N

N

R

R

S

S

A

A

L

S

V

I

R

R

V

I

P

P

M

V

Y

V

T

S

P

S

H

P

K

K

V

-

S

-

S

A

R

R

V

I

E

E

V

V

R

R

S

F

P

P

D

D

S

P

A

A

C

A

N

N

P

P

Y

Y

L

L

T

C

F

F

A

A

V

A

L

L

A

M

A

A

G

G

L

L

R

D

G

G

I

I

E

K

K

N

N

K

Y

I

V

H

L

P

G

G

P

E

Q

A

A

M

E

D

D

K

N

N

V

L

W
x
Y

S

D

L

L

T

P

P

P

E

E

R

A

R

K

A

E

M

I

G

-

Y

-

K

P

E

Q

L

V

P

A

G

G

S

S

L

L

G

E

V

E

A

A

L

L

T

N

E

E

M

L

E

D

N

L

S

D

E

R

L

E

V

F

A

L

E

K

A

A

L

G

G

G

-

V

-

F

-

T

E

D

H

E

V

A

F

I

D

D

F

A

F

Y

L

I

R

A

N

L

K

R

R

R

S

E

E

E

W

D

E

D

N

R

Y

V

R

R

S

M

H

T

V

P

T

H

P

P

F

V

E

E

L

F

K

E

N

L

Y

Y

L

Y

S

S

L

V

Y398 (≠ W376) modified: O-AMP-tyrosine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P0A1P6 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
35% identity, 99% coverage: 6:446/446 of query aligns to 4:469/469 of P0A1P6

query
sites
P0A1P6

E

E

F

H

V

V

L

L

R

T

T

M

L

L

E

N

E

E

R

H

D

E

I

V

R

K

F

F

V

V

R

D

L

L

W

R

F

F

T

T

D

D

V

T

L

K

G

G

Y

K

L

E

K

Q

S

H

V

V

A

T

I

I

A

P

P

A

A

H

E

Q

L

V

E

N

G

A

A

E

F

F

-

F

E

E

G

G

I

K

G

M

F

F

D

D

G

G

S

S

I

I

E

G

G

G

F

W

A

K

R

G

V

I

S

N

E

E

A

S

D

D

M

M

V

V

A

L

R

M

P

P

D

D

P

A

S

S

T

T

F

A

Q

V

V

I

L

D

P

P

W

F

T

F

T

A

S

D

A

S

G

-

N

-

H

-

Y

-

S

T

A

L

R

I

M

I

F

R

C

C

D

D

I

I

T

L

M

E

P

P

D

G

G

T

S

L

P

Q

S

G

W

Y

-

D

A

R

D

D

S

P

R

R

H

S

V

I

L

A

R

K

R

R

-

A

-

E

Q

D

L

Y

S

L

K

R

A

A

S

T

D

G

L

I

G

A

F

D

S

T

C

V

Y

L

V

F

H

G

P

P

E
|
E

I

P

E
|
E

F

F

L

L

L

F

K

D

-

D

-

I

-

R

-

F

-

G

-

A

-

S

-

I

-

S

-

G

-

S

-

H

-

V

-

A

-

I

-

D

-

I

-

E

-

G

-

A

-

W

-

N

-

S

-

T

-

K

-

Y

P

E

G

G

P

G

D

N

D

K

G

G

T

H

Q

R

P

P

I

-

P

-

A

G

D

V

N

K

G

G

Y

Y

F

F

D

P

Q

V

A

P

V

P

H

V

D

D

A

S

A

A

P

Q

N

D

F

I

R

R

R

S

H

E

A

M

I

C

D

L

A

V

L

M

E

E

Q

Q

M

M

G

G

I

L

S

V

V

V

E
|
E

F

A

S

H

H

H

E
|
E

G

V

A

A

P

T

-

A

G

G

Q

Q

Q

N

E
|
E

I

V

D

A

L

T

R

R

Y

F

A

N

D

T

A

M

L

T

S

K

M

K

A

A

D

D

N

E

V

I

M

Q

T

I

F

Y

R

K

Y

Y

L

V

V

V

K

H

E

N

V

V

A

A

L

H

A

R

D

F

G

G

V

K

R

T

A

A

S

T

F

F

M

M

P

P

K

K

P

P

F

M

A

F

E

G

H

D

P
x
N

G
|
G

S

S

A

G

M

M

H
|
H

T

C

H
|
H

M
|
M

S
|
S

L

L

F

A

E

K

G

N

D

G

T

T

N

N

A

L

F

F

H

-

S

S

P

G

D

D

D

K

P

Y

L

A

Q

G

L

L

S

S

D

E

V

Q

G

A

K

L

S

Y

F

Y

I

I

A

G

G

G

I

V

L

I

E

K

H

H

A

A

N

K

E

A

I

I

S

N

A

A

V

L

T

A

N

N

Q

P

W

T

V

T

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

H

P

G

G

G

Y

E

E

A

A

P

P

T

V

A

M

A

L

S

A

W

Y

G

S

A

A

A

R

N

N

R

R

S

S

A

A

L

S

V

I

R

R

V

I

P

P

M

V

Y

V

T

A

P

S

H

P

K

K

V

-

S

-

S

A

R

R

V

I

E
|
E

V

V

R
|
R

S

F

P

P

D

D

S

P

A

A

C

A

N

N

P

P

Y

Y

L

L

T

C

F

F

A

A

V

A

L

L

A

M

A

A

G

G

L

L

R

D

G

G

I

I

E

K

K

N

N

K

Y

I

V

H

L

P

G

G

P

E

Q

A

A

M

E

D

D

K

N

N

V

L

W

Y

S

D

L

L

T

P

P

P

E

E

R

A

R

K

A

E

M

I

G

-

Y

-

K

P

E

Q

L

V

P

A

G

G

S

S

L

L

G

E

V

E

A

A

L

L

T

N

E

A

M

L

E

D

N

L

S

D

E

R

L

E

V

F

A

L

E

K

A

A

L

G

G

G

-

V

-

F

-

T

E

D

H

E

V

A

F

I

D

D

F

A

F

Y

L

I

R

A

N

L

K

R

R

R

S

E

E

E

W

D

E

D

N

R

Y

V

R

R

S

M

H

T

V

P

T

H

P

P

F

V

E

E

L

F

K

E

N

L

Y

Y

L

Y

S

S

L

V

E130 (= E132) binding Mn(2+)
E132 (= E134) binding Mn(2+)
E208 (= E184) binding ATP
E213 (= E189) binding Mn(2+)
E221 (= E196) binding Mn(2+)
NG 265:266 (≠ PG 240:241) binding L-glutamate
H270 (= H245) binding Mn(2+)
HMS 272:274 (= HMS 247:249) binding ATP
R322 (= R298) binding L-glutamate
E358 (= E336) binding Mn(2+)
R360 (= R338) binding L-glutamate

Query Sequence

>WP_011780791.1 NCBI__GCF_000015305.1:WP_011780791.1
MDRQMEFVLRTLEERDIRFVRLWFTDVLGYLKSVAIAPAELEGAFEEGIGFDGSSIEGFA
RVSEADMVARPDPSTFQVLPWTTSAGNHYSARMFCDITMPDGSPSWADSRHVLRRQLSKA
SDLGFSCYVHPEIEFFLLKPGPDDGTQPIPADNGGYFDQAVHDAAPNFRRHAIDALEQMG
ISVEFSHHEGAPGQQEIDLRYADALSMADNVMTFRYLVKEVALADGVRASFMPKPFAEHP
GSAMHTHMSLFEGDTNAFHSPDDPLQLSDVGKSFIAGILEHANEISAVTNQWVNSYKRLV
HGGEAPTAASWGAANRSALVRVPMYTPHKVSSRRVEVRSPDSACNPYLTFAVLLAAGLRG
IEKNYVLGPQAEDNVWSLTPEERRAMGYKELPGSLGVALTEMENSELVAEALGEHVFDFF
LRNKRSEWENYRSHVTPFELKNYLSL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory