SitesBLAST

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
32% identity, 17% coverage: 276:400/725 of query aligns to 170:294/443 of 4lnkA

query
sites
4lnkA

D

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H

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S

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active site: E188 (= E294), E195 (= E301), H244 (= H350)
binding adenosine-5'-diphosphate: F198 (≠ P304), Y200 (≠ F306), N246 (= N352), S248 (= S354)
binding glutamic acid: E188 (= E294), V189 (= V295), N239 (= N345), G240 (= G346), G242 (= G348)
binding magnesium ion: E188 (= E294), E195 (= E301), H244 (= H350)

Sites not aligning to the query:

active site: 52, 131, 133, 315, 332, 334
binding adenosine-5'-diphosphate: 43, 50, 324, 328, 330
binding glutamic acid: 133, 303
binding magnesium ion: 131, 332

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
32% identity, 17% coverage: 276:400/725 of query aligns to 170:294/443 of 4lniA

query
sites
4lniA

D

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H

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S

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active site: E188 (= E294), E195 (= E301), H244 (= H350)
binding adenosine-5'-diphosphate: E183 (≠ K289), D197 (≠ A303), Y200 (≠ F306), N246 (= N352), S248 (= S354)
binding magnesium ion: E188 (= E294), E195 (= E301), E195 (= E301), H244 (= H350)
binding l-methionine-s-sulfoximine phosphate: E188 (= E294), H244 (= H350)

Sites not aligning to the query:

active site: 52, 131, 133, 315, 332, 334
binding adenosine-5'-diphosphate: 131, 320, 330
binding magnesium ion: 131, 131, 133, 332
binding l-methionine-s-sulfoximine phosphate: 133, 297, 303, 315, 334

P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 5 papers)
32% identity, 17% coverage: 276:400/725 of query aligns to 171:295/444 of P12425

query
sites
P12425

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E189 (= E294) binding Mg(2+)
V190 (= V295) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E301) binding Mg(2+)
G241 (= G346) binding L-glutamate
H245 (= H350) binding Mg(2+)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
59 G→R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
62 Important for inhibition by glutamine; R→A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.
132 binding Mg(2+)
134 binding Mg(2+)
302 G→E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
304 E→A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
306 P→H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
333 binding Mg(2+)
424 E→K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

4s0rD Structure of gs-tnra complex (see paper)
41% identity, 11% coverage: 276:355/725 of query aligns to 174:253/447 of 4s0rD

query
sites
4s0rD

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active site: E192 (= E294), E199 (= E301), H248 (= H350)
binding glutamine: E192 (= E294)
binding magnesium ion: E199 (= E301), H248 (= H350), H248 (= H350)
binding : G241 (= G343), V242 (= V344), N243 (= N345)

Sites not aligning to the query:

active site: 56, 135, 137, 319, 336, 338
binding glutamine: 137, 301, 307
binding magnesium ion: 66, 135, 135, 336, 419
binding : 63, 64, 65, 66, 161, 305, 306, 376, 426, 430

7tf6A Glutamine synthetase (see paper)
31% identity, 17% coverage: 280:400/725 of query aligns to 169:289/438 of 7tf6A

query
sites
7tf6A

E

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binding glutamine: E183 (= E294), G235 (= G346), H239 (= H350)
binding magnesium ion: E183 (= E294), E190 (= E301), H239 (= H350)
binding : G232 (= G343), N234 (= N345)

Sites not aligning to the query:

binding glutamine: 128, 292, 298
binding magnesium ion: 126, 128, 327
binding : 58, 60, 296, 297, 310, 367, 421, 433, 437

7tdvC Glutamine synthetase (see paper)
31% identity, 17% coverage: 280:400/725 of query aligns to 174:294/443 of 7tdvC

query
sites
7tdvC

E

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binding adenosine-5'-diphosphate: E183 (≠ K289), D197 (≠ A303), F198 (≠ P304), K199 (≠ M305), Y200 (≠ F306), N246 (= N352), V247 (≠ F353), S248 (= S354)
binding magnesium ion: E188 (= E294), E195 (= E301), E195 (= E301), H244 (= H350)
binding l-methionine-s-sulfoximine phosphate: E188 (= E294), E195 (= E301), G240 (= G346), H244 (= H350)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 129, 131, 320, 328, 330
binding magnesium ion: 131, 131, 133, 332
binding l-methionine-s-sulfoximine phosphate: 131, 133, 297, 303, 315

7tfaB Glutamine synthetase (see paper)
41% identity, 10% coverage: 280:355/725 of query aligns to 172:247/441 of 7tfaB

query
sites
7tfaB

E

K

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binding glutamine: E186 (= E294), G238 (= G346), H242 (= H350)
binding magnesium ion: E186 (= E294), E193 (= E301), H242 (= H350)
binding : V187 (= V295), N237 (= N345)

Sites not aligning to the query:

7tdpA Structure of paenibacillus polymyxa gs bound to met-sox-p-adp (transition state complex) to 1.98 angstom (see paper)
41% identity, 10% coverage: 280:355/725 of query aligns to 170:245/439 of 7tdpA

query
sites
7tdpA

E

K

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binding adenosine-5'-diphosphate: E179 (≠ K289), D193 (≠ A303), Y196 (≠ F306), N242 (= N352), S244 (= S354)
binding magnesium ion: E184 (= E294), E191 (= E301), E191 (= E301), H240 (= H350)
binding l-methionine-s-sulfoximine phosphate: E184 (= E294), E191 (= E301), G236 (= G346), H240 (= H350)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 123, 125, 127, 316, 326
binding magnesium ion: 127, 127, 129, 328
binding l-methionine-s-sulfoximine phosphate: 127, 129, 293, 299, 311, 330

8oooA Glutamine synthetase from methanothermococcus thermolithotrophicus in complex with 2-oxoglutarate and mgatp at 2.15 a resolution (see paper)
31% identity, 19% coverage: 219:355/725 of query aligns to 132:254/447 of 8oooA

query
sites
8oooA

F

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E

-

L

-

F

-

K

-

L

L

G

G

I

F

P

H

A

V

K
x
E

T

A

R
x
S

H

H

N

H

E

E

V

V

A

A

P

P

A

G

Q

Q

F

H

E

E

V

V

A

D

P
x
F

M
x
K

F
|
F

E

D

R

D

A

A

N

L

I

K

A

T

S

A

D

D

H

S

Q

V

Q

I

L

T

L

F

M

K

T

T

V

T

F

I

K

K

T

T

V

I

A

A

K

E

K

Q

H

H

G

G

M

L

E

K

C

A

L

T

F

F

H

M

E

P

K

K

P

P

F

F

A

F

G

G

V

M

N

N

G

G

S

S

G

G

K

M

H

H

V

C

N
x
H

F

Q

S
|
S

V

I

binding 2-oxoglutaric acid: P170 (= P267), R173 (vs. gap), R174 (= R269), S190 (≠ R291)
binding adenosine-5'-triphosphate: E136 (= E223), E188 (≠ K289), F203 (≠ P304), K204 (≠ M305), F205 (= F306), H251 (≠ N352), S253 (= S354)

Sites not aligning to the query:

8ooqB Glutamine synthetase from Methanothermococcus thermolithotrophicus (see paper)
31% identity, 19% coverage: 219:355/725 of query aligns to 131:253/446 of 8ooqB

query
sites
8ooqB

F

F

C

V

G

G

P

P

E

E

Q

P

E
|
E

Y

F

F

F

L

L

V

L

D

K

R

K

-

D

-

P

-

H

-

N

-

P

H

H

F

K

F

Y

L

I

A

P

R

A

P

D

D

D

L

-

V

-

N

-

A

-

G

-

R

-

T

-

L

-

F

-

G

-

A

-

K

-

P

-

K

-

G

G

Q

G

E

Y

F

F

D

D

D

L

H

E

Y

P

F

M

G

D

A

E

V

A

P
|
P

E

D

-

I

-
x
R

R
|
R

V

D

L

I

G

V

F

F

M

A

M

L

D

E

T

N

E

-

R

-

E

-

L

-

F

-

K

-

L

L

G

G

I

F

P

H

A

V

K

E

T

A

R
x
S

H

H

N

H

E
|
E

V

V

A

A

P

P

A

G

Q

Q

F

H

E
|
E

V

V

A

D

P

F

M

K

F

F

E

D

R

D

A

A

N

L

I

K

A

T

S

A

D

D

H

S

Q

V

Q

I

L

T

L

F

M

K

T

T

V

T

F

I

K

K

T

T

V

I

A

A

K

E

K

Q

H

H

G

G

M

L

E

K

C

A

L

T

F

F

H

M

E

P

K

K

P

P

F

F

A

F

G

G

V

M

N

N

G

G

S

S

G

G

K

M

H

H

V

C

N

H

F

Q

S

S

V

I

binding 2-oxoglutaric acid: P169 (= P267), R172 (vs. gap), R173 (= R269), S189 (≠ R291)
binding magnesium ion: E137 (= E225), E192 (= E294), E199 (= E301)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 16, 18, 32, 86, 92

5zlpJ Crystal structure of glutamine synthetase from helicobacter pylori (see paper)
28% identity, 31% coverage: 217:440/725 of query aligns to 130:360/478 of 5zlpJ

query
sites
5zlpJ

V

V

S

A

F
x
Y

C

F

G

G

P

A

E
|
E

Q

N

E
|
E

Y

F

F

F

L

I

V

F

D

D

R

S

H

-

F

-

L

I

A

K

R

I

P

K

D

D

L

A

V

S

N

N

A

S

G

-

R

-

T

Q

L

Y

F

Y

G

E

A

V

K

D

P

S

P

E

K

E

G

G

Q

E

E

W

F

N

D

R

D

D

H

R

Y

S

F

F

-

E

-

N

-

G

-

V

-

N

-

F

G

G

A

H

V

R

P

P

E

G

R

K

V

Q

L

G

G

G

F

Y

M

M

-

P

-

V

-

P

-

T

-

D

-

T

-

M

M

M

D

D

T

I

E

R

R

T

E

E

L

I

F

V

K

K

L

V

-

L

-

N

-

Q

-

V

G

G

I

L

P

E

A

T

K
x
F

T

V

R

V

H

H

N

H

E
|
E

V

V

A

A

P

Q

A

A

Q

Q

F

G

E
|
E

V

V

A

G

P

V

M

K

F
|
F

E

G

R

D

A

L

N

V

I

E

A

A

S

A

D

D

H

N

Q

V

Q

Q

L

K

L

L

M

K

T

Y

V

V

F

V

K

K

T

M

V

V

A

A

K

H

K

L

H

N

G

G

M

K

E

T

C

A

L

T

F

F

H

M

E

P

K

K

P

P

F

L

A

Y

G

G

V

D

N

N

G
|
G

S

S

G

G

K

M

H
|
H

V

T

N
x
H

F

V

S
|
S

V

V

G

W

N

K

A

N

D

N

L

E

G

N

S

-

L

-

L

L

V

F

P

S

G

G

D

E

T

T

P

Y

H

K

E

G

N

L

A

S

Q

E

F

F

-

A

L

L

V

H

F

F

C

L

A

G

I

V

I

L

R

R

A

H

V

A

H

R

K

-

F

-

G

-

G

G

L

L

L

A

R

A

V

F

S

T

V

N

A

A

S

S

A

T

T

N

N

S

D

Y

H

K

R

R

L

L

G

I

A

P

N

G

E

Y

A
x
E

P

A

P

P

A

S

I

I

I

L

S

T

I

Y

F

S

L

A

G

N

E

N

Q
x
R

L

S

A

A

D

S

V

V

F

-

D

-

Q
x
R

I

I

A

P

K

Y

G

G

A

I

A

S

T

K

S

N

S

S

binding adenosine-5'-diphosphate: Y132 (≠ F219), E136 (= E223), F215 (≠ K289), F232 (= F306), H278 (≠ N352), S280 (= S354), R351 (≠ Q431)
binding magnesium ion: E138 (= E225), E220 (= E294), E227 (= E301)
binding phosphinothricin: E138 (= E225), E220 (= E294), G272 (= G346), H276 (= H350), E334 (≠ A412), R346 (≠ Q424)

Sites not aligning to the query:

5zlpL Crystal structure of glutamine synthetase from helicobacter pylori (see paper)
28% identity, 31% coverage: 217:440/725 of query aligns to 128:358/476 of 5zlpL

query
sites
5zlpL

V

V

S

A

F

Y

C

F

G
|
G

P

A

E
|
E

Q

N

E
|
E

Y

F

F

F

L

I

V

F

D

D

R

S

H

-

F

-

L

I

A

K

R

I

P

K

D

D

L

A

V

S

N

N

A

S

G

-

R

-

T

Q

L

Y

F

Y

G

E

A

V

K

D

P

S

P

E

K

E

G

G

Q

E

E

W

F

N

D

R

D

D

H

R

Y

S

F

F

-

E

-

N

-

G

-

V

-

N

-

F

G

G

A

H

V

R

P

P

E

G

R

K

V

Q

L

G

G

G

F

Y

M

M

-

P

-

V

-

P

-

T

-

D

-

T

-

M

M

M

D

D

T

I

E

R

R

T

E

E

L

I

F

V

K

K

L

V

-

L

-

N

-

Q

-

V

G

G

I

L

P

E

A

T

K
x
F

T

V

R

V

H

H

N

H

E
|
E

V

V

A

A

P

Q

A

A

Q

Q

F

G

E
|
E

V

V

A

G

P

V

M

K

F
|
F

E

G

R

D

A

L

N

V

I

E

A

A

S

A

D

D

H

N

Q

V

Q

Q

L

K

L

L

M

K

T

Y

V

V

F

V

K

K

T

M

V

V

A

A

K

H

K

L

H

N

G

G

M

K

E

T

C

A

L

T

F

F

H

M

E

P

K

K

P

P

F

L

A

Y

G

G

V

D

N

N

G
|
G

S

S

G

G

K

M

H
|
H

V

T

N
x
H

F

V

S
|
S

V

V

G

W

N

K

A

N

D

N

L

E

G

N

S

-

L

-

L

L

V

F

P

S

G

G

D

E

T

T

P

Y

H

K

E

G

N

L

A

S

Q

E

F

F

-

A

L

L

V

H

F

F

C

L

A

G

I

V

I

L

R

R

A

H

V

A

H

R

K

-

F

-

G

-

G

G

L

L

L

A

R

A

V

F

S

T

V

N

A

A

S

S

A

T

T

N

N

S

D

Y

H

K

R

R

L

L

G

I

A

P

N

G

E

Y

A
x
E

P

A

P

P

A

S

I

I

I

L

S

T

I

Y

F

S

L

A

G

N

E

N

Q
x
R

L

S

A

A

D

S

V

V

F

-

D

-

Q
x
R

I

I

A

P

K

Y

G

G

A

I

A

S

T

K

S

N

S

S

binding adenosine-5'-diphosphate: G132 (= G221), E134 (= E223), F213 (≠ K289), F230 (= F306), H276 (≠ N352), S278 (= S354), R349 (≠ Q431)
binding magnesium ion: E136 (= E225), E218 (= E294), E225 (= E301)
binding (2s)-2-amino-4-[methyl(phosphonooxy)phosphoryl]butanoic acid: E136 (= E225), E218 (= E294), G270 (= G346), H274 (= H350), E332 (≠ A412), R344 (≠ Q424)

Sites not aligning to the query:

5zlpA Crystal structure of glutamine synthetase from helicobacter pylori (see paper)
28% identity, 31% coverage: 217:440/725 of query aligns to 128:358/476 of 5zlpA

query
sites
5zlpA

V

V

S

A

F

Y

C

F

G

G

P

A

E
|
E

Q

N

E

E

Y

F

F

F

L

I

V

F

D

D

R

S

H

-

F

-

L

I

A

K

R

I

P

K

D

D

L

A

V

S

N

N

A

S

G

-

R

-

T

Q

L

Y

F

Y

G

E

A

V

K

D

P

S

P

E

K

E

G

G

Q

E

E

W

F

N

D

R

D

D

H

R

Y

S

F

F

-

E

-

N

-

G

-

V

-

N

-

F

G

G

A

H

V

R

P

P

E

G

R

K

V

Q

L

G

G

G

F

Y

M

M

-

P

-

V

-

P

-

T

-

D

-

T

-

M

M

M

D

D

T

I

E

R

R

T

E

E

L

I

F

V

K

K

L

V

-

L

-

N

-

Q

-

V

G

G

I

L

P

E

A

T

K

F

T

V

R

V

H

H

N

H

E

E

V

V

A

A

P

Q

A

A

Q

Q

F

G

E

E

V

V

A

G

P
x
V

M

K

F
|
F

E

G

R

D

A

L

N

V

I

E

A

A

S

A

D

D

H

N

Q

V

Q

Q

L

K

L

L

M

K

T

Y

V

V

F

V

K

K

T

M

V

V

A

A

K

H

K

L

H

N

G

G

M

K

E

T

C

A

L

T

F

F

H

M

E

P

K

K

P

P

F

L

A

Y

G

G

V

D

N

N

G

G

S

S

G

G

K

M

H

H

V

T

N
x
H

F

V

S

S

V

V

G

W

N

K

A

N

D

N

L

E

G

N

S

-

L

-

L

L

V

F

P

S

G

G

D

E

T

T

P

Y

H

K

E

G

N

L

A

S

Q

E

F

F

-

A

L

L

V

H

F

F

C

L

A

G

I

V

I

L

R

R

A

H

V

A

H

R

K

-

F

-

G

-

G

G

L

L

L

A

R

A

V

F

S

T

V

N

A

A

S

S

A

T

T

N

N

S

D

Y

H

K

R

R

L

L

G

I

A

P

N

G

E

Y

A

E

P

A

P

P

A

S

I

I

I

L

S

T

I

Y

F

S

L

A

G

N

E

N

Q

R

L

S

A

A

D

S

V

V

F

-

D

-

Q

R

I

I

A

P

K

Y

G

G

A

I

A

S

T

K

S

N

S
|
S

binding adenosine-5'-triphosphate: E134 (= E223), V228 (≠ P304), F230 (= F306), H276 (≠ N352), S358 (= S440)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 360

8tfkA Glutamine synthetase (see paper)
31% identity, 18% coverage: 262:395/725 of query aligns to 152:276/440 of 8tfkA

query
sites
8tfkA

Y

Y

F

F

G

D

A

F

V

A

P

P

-

L

E

D

R

R

V

A

L

Q

G

D

F

V

M

R

D

D

T

I

E

D

R

Y

E

A

L

L

F

E

K

H

L

M

G

G

I

F

P

Q

A

I

K

E

T

A

R

S

H

H

N

H

E
|
E

V

V

A

A

P

P

A

S

Q

Q

F

H

E
|
E

V

I

A
x
D

P
x
F

M

R

F
|
F

E

G

R

D

A

V

N

L

I

C

A

T

S

A

D

D

H

N

Q

V

L

T

L

F

M

K

T

Y

V

V

F

V

K

K

T

S

V

I

A

A

K

Y

K

H

H

K

G

G

M

Y

E

Y

C

A

L

S

F

F

H

M

E

P

K

K

P

P

F

L

A

F

G

G

V

V

N

N

G
|
G

S

S

G

G

K

M

H
|
H

V

S

N
|
N

F

Q

S

S

V

L

G

F

N

K

A

-

D

D

L

G

G

K

S

N

L

V

L

F

V

Y

P

D

G

P

D

D

T

T

P

P

H

T

E

K

N

L

A

S

Q

Q

F

-

L

-

V

-

F

-

C

-

A

-

I

-

R

D

A

A

V

M

H

Y

K

Y

F

I

G

G

L

L

R

K

binding adenosine-5'-diphosphate: D194 (≠ A303), F195 (≠ P304), F197 (= F306), N243 (= N352)
binding magnesium ion: E185 (= E294), E192 (= E301), E192 (= E301), H241 (= H350)
binding l-methionine-s-sulfoximine phosphate: E185 (= E294), E192 (= E301), G237 (= G346), H241 (= H350)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 128, 312, 317, 325, 327
binding magnesium ion: 128, 128, 130, 329
binding l-methionine-s-sulfoximine phosphate: 128, 130, 294, 300, 312, 331

8oozA Glutamine synthetase (see paper)
29% identity, 19% coverage: 221:355/725 of query aligns to 117:236/430 of 8oozA

query
sites
8oozA

G
|
G

P

P

E
|
E

Q

M

E

E

Y

F

F

F

L

L

V

F

D

K

R

R

H

Q

F

-

L

-

A

-

R

-

P

-

D

-

L

-

V

-

N

-

A

-

G

-

R

-

T

-

L

-

F

-

G

D

A

G

K

M

P

P

P

T

K

N

G

I

Q

P

E

Q

F

D

D

R

D

G

H

G

Y

Y

F

F

G

D

A

L

V

A

P

P

E

-

R

-

V

-

L

I

G

D

F

L

M

A

M

E

D

E

T

I

E

K

R

R

E

E

L

I

F

V

-

L

-

V

-

L

-

E

K

E

L

M

G

G

I

F

P

E

A

V

K
x
E

T

A

R

A

H

H

N

H

E

E

V

V

A

A

P

F

A

G

Q

Q

F

H

E

E

V

I

A

D

P
x
F

M
x
K

F
x
Y

E

D

R

N

A

A

N

L

I

A

A

T

S

A

D

D

H

N

Q

V

Q

I

L

T

L

L

M

K

T

Y

V

V

F

A

K

K

T

T

V

L

A

A

K

L

K

Q

H

H

G

G

M

L

E

H

C

A

L

T

F

F

H

M

E

P

K

K

P

P

F

I

A

F

G

G

V

V

N

N

G

G

S

S

G

G

K

M

H
|
H

V

T

N
|
N

F

T

S
|
S

V

L

binding adenosine-5'-triphosphate: G117 (= G221), E170 (≠ K289), F185 (≠ P304), K186 (≠ M305), Y187 (≠ F306), N233 (= N352), S235 (= S354)
binding magnesium ion: E119 (= E223), H231 (= H350)

Sites not aligning to the query:

8ooxB Glutamine synthetase (see paper)
29% identity, 19% coverage: 221:355/725 of query aligns to 125:244/438 of 8ooxB

query
sites
8ooxB

G

G

P

P

E

E

Q

M

E
|
E

Y

F

F

F

L

L

V

F

D

K

R

R

H

Q

F

-

L

-

A

-

R

-

P

-

D

-

L

-

V

-

N

-

A

-

G

-

R

-

T

-

L

-

F

-

G

D

A

G

K

M

P

P

P

T

K

N

G

I

Q

P

E

Q

F

D

D

R

D

G

H

G

Y

Y

F

F

G

D

A

L

V

A

P

P

E

-

R

-

V

-

L

I

G

D

F

L

M

A

M

E

D

E

T

I

E

K

R

R

E

E

L

I

F

V

-

L

-

V

-

L

-

E

K

E

L

M

G

G

I

F

P

E

A

V

K

E

T

A

R

A

H

H

N

H

E
|
E

V

V

A

A

P

F

A

G

Q

Q

F

H

E
|
E

V

I

A

D

P

F

M

K

F

Y

E

D

R

N

A

A

N

L

I

A

A

T

S

A

D

D

H

N

Q

V

Q

I

L

T

L

L

M

K

T

Y

V

V

F

A

K

K

T

T

V

L

A

A

K

L

K

Q

H

H

G

G

M

L

E

H

C

A

L

T

F

F

H

M

E

P

K

K

P

P

F

I

A

F

G

G

V

V

N

N

G

G

S

S

G

G

K

M

H

H

V

T

N

N

F

T

S

S

V

L

binding magnesium ion: E129 (= E225), E183 (= E294), E190 (= E301)

7cqwA Gmas/adp complex-conformation 1 (see paper)
30% identity, 19% coverage: 229:368/725 of query aligns to 121:252/430 of 7cqwA

query
sites
7cqwA

V

V

D

E

R

C

H

E

F

F

L

L

A

I

R

Q

P

P

D

D

-

G

-

S

-

A

L

I

V

S

N

D

A

P

G

A

R

D

T

T

L

-

F

-

G

Q

A

A

K

K

P

P

P

C

K

Y

G

D

Q

Q

E

D

F

A

D

L

D

M

H

R

Y

R

F

F

G

D

A

V

V

I

P

A

E

E

R

-

V

I

L

C

G

S

F

Y

M

M

M

V

D

D

T

-

E

-

R

-

E

-

L

-

F

-

K

-

L

L

G

G

I

W

P

G

A

P

K
x
Y

T

Q

R

N

H

D

N

H

E

E

V

D

A

A

P

N

A

G

Q

Q

F

F

E

E

V

M

A

N

P

W

M

D

F
x
Y

E

A

R

D

A

A

N

L

I

V

A

T

S

A

D

D

H

R

Q

H

Q

A

L

F

M

K

T

F

V

M

F

V

K

K

T

S

V

V

A

A

K

E

K

R

H

H

G

G

M

L

E

R

C

A

L

T

F

F

H

M

E

P

K

K

P

P

F

F

A

A

G

H

V

L

N

T

G

G

S

N

G

G

K

C

H

H

V

T

N
x
H

F
x
L

S
|
S

V

M

G

W

N

T

A

A

D

-

L

A

G

G

S

D

L

N

L

L

V

F

P

E

G

G

D

D

binding adenosine-5'-diphosphate: Y174 (≠ K289), Y191 (≠ F306), H237 (≠ N352), L238 (≠ F353), S239 (= S354)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 317, 323

Query Sequence

>WP_011781186.1 NCBI__GCF_000015305.1:WP_011781186.1
MSGNAVRLQAINNVEAYVPPAISFDPTEAPGQIFGSNVFTKAEMQLRLPKSVYKSVVATI
EKGAKLDPAIADAVASAMKDWALSKGATHYAHVFYPMTGLTAEKHDSFLEPISDGETLAE
FAGKTLIQGEPDASSFPSGGLRSTFEARGYTGWDVTSPAYILENPNGNTLCIPTVFVSMT
GEALDYKTPLLRSQQAMGVHAERILALFGHDNLQKVVSFCGPEQEYFLVDRHFFLARPDL
VNAGRTLFGAKPPKGQEFDDHYFGAVPERVLGFMMDTERELFKLGIPAKTRHNEVAPAQF
EVAPMFERANIASDHQQLLMTVFKTVAKKHGMECLFHEKPFAGVNGSGKHVNFSVGNADL
GSLLVPGDTPHENAQFLVFCAAIIRAVHKFGGLLRVSVASATNDHRLGANEAPPAIISIF
LGEQLADVFDQIAKGAATSSKGKGVMHIGVDTLPTLPTDPGDRNRTSPFAFTGNRFEFRA
PGSGQTVAVPMIILNTIMADSFDYMATALEKAVAGGVDFDSAVQTLLTEIITEHGAVVFN
GDGYSENWQIEAAERGLPNLKTTLDAIPELIKPAAVEVFERYGVFSERELHSRYEVRLEQ
YALTIAVEAKLALELGTTVILPAAIRYQTELAQNVATLKAAGMDADTTLLQAVSAPIAEL
TTALGALKAALSGHSGETALDEATHAQQALLPAMEAVRTAADTLETVVADDLWPLPTYQE
MLYIL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory