SitesBLAST
Comparing WP_011799471.1 NCBI__GCF_000015505.1:WP_011799471.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q936X2 Allophanate hydrolase; EC 3.5.1.54 from Pseudomonas sp. (strain ADP) (see paper)
51% identity, 96% coverage: 27:599/600 of query aligns to 38:604/605 of Q936X2
- K91 (= K77) mutation to A: Loss of activity.
- S165 (= S151) mutation to A: Loss of activity.
- S189 (= S175) mutation to A: Loss of activity.
4gysB Granulibacter bethesdensis allophanate hydrolase co-crystallized with malonate (see paper)
52% identity, 71% coverage: 28:450/600 of query aligns to 18:442/461 of 4gysB
- active site: K72 (= K77), S146 (= S151), S147 (= S152), T165 (= T170), T167 (= T172), A168 (= A173), G169 (= G174), S170 (= S175), V173 (= V178)
- binding malonate ion: A120 (= A125), G122 (= G127), S146 (= S151), T167 (= T172), A168 (= A173), S170 (= S175), S193 (≠ Q198), G194 (= G199), V195 (= V200), R200 (= R205), Y297 (= Y305), R305 (= R313)
Q7XJJ7 Fatty acid amide hydrolase; AtFAAH; N-acylethanolamine amidohydrolase; EC 3.5.1.99 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
28% identity, 71% coverage: 16:442/600 of query aligns to 137:584/607 of Q7XJJ7
- K205 (= K77) mutation to A: Loss of activity.
- SS 281:282 (= SS 151:152) mutation to AA: Loss of activity.
- GGGS 302:305 (≠ TAGS 172:175) binding substrate
- S305 (= S175) mutation to A: Loss of activity.
- R307 (= R177) mutation to A: Loss of activity.
- S360 (≠ Y230) mutation to A: No effect.
6diiH Structure of arabidopsis fatty acid amide hydrolase in complex with methyl linolenyl fluorophosphonate (see paper)
28% identity, 71% coverage: 16:442/600 of query aligns to 137:584/616 of 6diiH
- binding methyl-9Z,12Z,15Z-octadecatrienylphosphonofluoridate: G255 (≠ A125), T258 (≠ L128), S281 (= S151), G302 (≠ T172), G303 (≠ A173), S305 (= S175), S472 (≠ T336), I532 (= I390), M539 (≠ G397)
Sites not aligning to the query:
8ey9B Structure of arabidopsis fatty acid amide hydrolase mutant s305a in complex with 9-hydroxy-10,12-octadecadienoyl-ethanolamide
28% identity, 71% coverage: 16:442/600 of query aligns to 137:584/605 of 8ey9B
- binding (9R,10E,12Z)-9-hydroxy-N-(2-hydroxyethyl)octadeca-10,12-dienamide: G255 (≠ A125), G302 (≠ T172), G303 (≠ A173), G304 (= G174), A305 (≠ S175), V442 (≠ E306), I475 (≠ G339), M539 (≠ G397)
Sites not aligning to the query:
8ey1D Structure of arabidopsis fatty acid amide hydrolase mutant s305a in complex with n-(3-oxododecanoyl)-l-homoserine lactone
28% identity, 71% coverage: 16:442/600 of query aligns to 137:584/605 of 8ey1D
3h0mA Structure of tRNA-dependent amidotransferase gatcab from aquifex aeolicus (see paper)
28% identity, 74% coverage: 9:450/600 of query aligns to 6:470/478 of 3h0mA
- active site: K72 (= K77), S147 (= S151), S148 (= S152), S166 (≠ T170), T168 (= T172), G169 (≠ A173), G170 (= G174), S171 (= S175), Q174 (≠ V178)
- binding glutamine: M122 (≠ T126), G123 (= G127), D167 (= D171), T168 (= T172), G169 (≠ A173), G170 (= G174), S171 (= S175), F199 (≠ A203), Y302 (vs. gap), R351 (≠ I335), D418 (≠ N401)
3h0lA Structure of tRNA-dependent amidotransferase gatcab from aquifex aeolicus (see paper)
28% identity, 74% coverage: 9:450/600 of query aligns to 6:470/478 of 3h0lA
- active site: K72 (= K77), S147 (= S151), S148 (= S152), S166 (≠ T170), T168 (= T172), G169 (≠ A173), G170 (= G174), S171 (= S175), Q174 (≠ V178)
- binding asparagine: G123 (= G127), S147 (= S151), G169 (≠ A173), G170 (= G174), S171 (= S175), Y302 (vs. gap), R351 (≠ I335), D418 (≠ N401)
1m21A Crystal structure analysis of the peptide amidase pam in complex with the competitive inhibitor chymostatin (see paper)
31% identity, 64% coverage: 68:452/600 of query aligns to 72:482/487 of 1m21A
- active site: K81 (= K77), S160 (= S151), S161 (= S152), T179 (= T170), T181 (= T172), D182 (≠ A173), G183 (= G174), S184 (= S175), C187 (≠ V178)
- binding : A129 (= A125), N130 (≠ T126), F131 (vs. gap), C158 (≠ G149), G159 (= G150), S160 (= S151), S184 (= S175), C187 (≠ V178), I212 (≠ A203), R318 (= R302), L321 (= L303), L365 (≠ P342), F426 (= F396)
Q9FR37 Amidase 1; AtAMI1; Translocon at the outer membrane of chloroplasts 64-I; AtTOC64-I; EC 3.5.1.4 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
43% identity, 29% coverage: 69:242/600 of query aligns to 28:200/425 of Q9FR37
- K36 (= K77) active site, Charge relay system; mutation to A: Loss of catalytic activity.; mutation to R: Reduces catalytic activity 10-fold.
- S113 (= S151) active site, Charge relay system; mutation S->A,T: Loss of catalytic activity.
- S114 (= S152) mutation to A: Loss of catalytic activity.; mutation to T: Reduces catalytic activity 400-fold.
- D133 (= D171) mutation to A: Loss of catalytic activity.; mutation to E: Reduces catalytic activity 600-fold.
- S137 (= S175) active site, Acyl-ester intermediate; mutation to A: Reduces catalytic activity 170-fold.; mutation to T: Loss of catalytic activity.
- C145 (≠ N183) mutation C->A,S: Reduces catalytic activity 10-fold.
Sites not aligning to the query:
- 214 S→T: Slightly reduces catalytic activity.
3kfuE Crystal structure of the transamidosome (see paper)
32% identity, 64% coverage: 66:448/600 of query aligns to 54:456/468 of 3kfuE