SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011801935.1 NCBI__GCF_000015505.1:WP_011801935.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O53289 Phosphoserine phosphatase SerB2; PSP; PSPase; O-phosphoserine phosphohydrolase; Protein-serine/threonine phosphatase; EC 3.1.3.3; EC 3.1.3.16 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
43% identity, 95% coverage: 12:235/236 of query aligns to 165:384/409 of O53289

query
sites
O53289

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D185 (= D30) mutation to G: Completely abolishes enzymatic activity.; mutation to N: Completely abolishes enzymatic activity.
V186 (≠ M31) mutation to Q: Decreases enzymatic activity by 50%.
D187 (= D32) mutation to N: Decreases enzymatic activity by 15%.
S188 (= S33) mutation to A: No effect on enzymatic activity.
S273 (= S120) mutation to A: Completely abolishes enzymatic activity (PubMed:25521849). Decreases enzymatic activity by 60% (PubMed:25037224).
K318 (= K169) mutation to A: Decreases enzymatic activity by 50%.; mutation to E: Completely abolishes enzymatic activity.
D341 (= D192) mutation to G: Decreases enzymatic activity by 80%.; mutation to N: Decreases enzymatic activity by 85%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-345.
D345 (= D196) mutation to N: Decreases enzymatic activity by 55%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-341.

Sites not aligning to the query:

18 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.
108 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.

7qplA Crystal structure of phosphoserine phosphatase (serb) from brucella melitensis in complex with phosphate and magnesium
43% identity, 95% coverage: 9:232/236 of query aligns to 65:283/295 of 7qplA

query
sites
7qplA

G

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binding magnesium ion: D86 (= D30), D88 (= D32), D243 (= D192)

A0QJI1 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Mycobacterium avium (strain 104) (see paper)
40% identity, 95% coverage: 12:235/236 of query aligns to 167:386/411 of A0QJI1

query
sites
A0QJI1

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D187 (= D30) binding
D189 (= D32) binding
D343 (= D192) binding

5jlpA Crystal structure of mycobacterium avium serb2 in complex with serine at act domain
40% identity, 95% coverage: 12:235/236 of query aligns to 163:382/396 of 5jlpA

query
sites
5jlpA

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active site: D183 (= D30), V184 (≠ M31), D185 (= D32), G272 (= G121), K316 (= K169), D343 (= D196)
binding magnesium ion: D183 (= D30), D339 (= D192)

Sites not aligning to the query:

binding serine: 13, 14

8a21A Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with phenylimidazole (see paper)
40% identity, 95% coverage: 12:235/236 of query aligns to 163:382/396 of 8a21A

query
sites
8a21A

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binding magnesium ion: D183 (= D30), D185 (= D32), D339 (= D192)
binding 4-phenyl-1h-imidazole: D185 (= D32), E192 (= E39), V193 (≠ C40), I194 (≠ V41), T211 (= T58), M215 (= M62), F221 (= F69), R228 (= R76), G273 (= G122)

Sites not aligning to the query:

binding 4-phenyl-1h-imidazole: 13, 18, 37

8a1zA Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with 1-(2,4-dichlorophenyl)-3-hydroxyurea (see paper)
40% identity, 95% coverage: 12:235/236 of query aligns to 163:382/396 of 8a1zA

query
sites
8a1zA

V

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M

I

L

A

A

D

A

A

K

V

A

G

G

R

A

K

E

A

G

E

Q

V

V

A

A

I

I

T

T

E

D

A

A

M
|
M

R

R

G

G

E

E

I

L

T

-

D

D

F
|
F

K

A

D

Q

S

S

L
|
L

R

Q

R
|
R

V

V

A

A

L

T

L

L

K

A

G

G

V

L

S

P

M

A

A

T

S

V

M

I

D

D

E

E

V

V

Y

A

R

G

E

Q

R

-

L

L

K

E

L

L

N

M

P

P

G

G

A

A

A

R

E

T

L

T

V

L

R

R

A

T

C

L

K

R

D

R

A

L

G

G

M

Y

K

A

I

C

L

G

L

V

V

V

S

S

G
|
G

G

G

F
|
F

T

R

Y

R

F

I

T

I

D

E

R

P

V

L

K

A

G

E

L

E

L

L

D

M

I

L

D

D

F

Y

T

V

R

A

S

A

N

N

V

E

L

L

E

E

V

I

R

V

D

D

G

G

L

T

L

L

T

T

G

G

K

R

M

V

I

V

D

-

Q

-

S

-

W

-

G

G

D

P

I

I

C

I

D

D

G

R

E

A

E

G

K

K

R

A

R

T

M

A

L

L

I

R

Q

E

T

F

C

A

G

Q

Q

R

L

A

G

G

I

V

N

P

P

M

L

A

Q

Q

A

T

I

V

A

A

M

V

G

G

D
|
D

G

G

A

A

N

N

D

D

L

I

P

D

M

M

M

L

G

A

A

A

G

G

L

L

S

G

V

I

A

A

Y

F

H

N

A

A

K

K

P

P

K

A

V

L

R

R

E

E

Q

V

A

A

M

D

V

A

A

S

I

L

N

S

A

H

G

P

G

Y

L

L

D

D

R

T

L

V

L

L

E

F

L

L

M

L

binding 1-(2,4-dichlorophenyl)-3-oxidanyl-urea: D185 (= D32), E192 (= E39), M215 (= M62), F221 (= F69), L225 (= L73), R228 (= R76), G272 (= G121), F274 (= F123), D339 (= D192)
binding magnesium ion: D183 (= D30), D185 (= D32), D339 (= D192)

1f5sA Crystal structure of phosphoserine phosphatase from methanococcus jannaschii (see paper)
38% identity, 90% coverage: 25:236/236 of query aligns to 5:210/210 of 1f5sA

query
sites
1f5sA

K

K

L

L

I

I

A

L

F

F

D
|
D

M
x
F

D
|
D

S

S

T

T

L

L

I

V

N

N

I

N

E

E

C

T

V

I

D

D

E

E

I

I

A

A

D

R

A

E

V

A

G

G

R

V

K

E

A

E

E

E

V

V

A

K

I

I

T

T

E

K

A

E

A

A

M

M

R

E

G

G

E

K

I

L

T

-

D

N

F

F

K

E

D

Q

S

S

L

L

R

R

R

K

R

R

V

V

A

S

L

L

K

K

G

D

V

L

S

P

M

I

A

E

S

K

M

V

D

E

E

K

V

A

Y

I

R

K

E

-

R

R

L

I

K

T

L

P

N

T

P

E

G

G

A

A

A

E

E

E

L

T

V

I

R

K

A

E

C

L

K

K

D

N

A

R

G

G

M

Y

K

V

I

V

L

A

L

V

V

V

S
|
S

G
|
G

G

G

F

F

T

D

Y

I

F

A

T

V

D

N

R

K

V

I

K

K

G

E

L

K

L

L

D

G

I

L

D

D

F

Y

T

A

R

F

S

A

N

N

V

R

L

L

E

I

V

V

R

K

D

D

G

G

L

K

L

L

T

T

G

G

K

D

M

V

I

-

D

-

Q

-

S

-

W

E

G

G

D

E

I

V

C

L

D

K

G

E

E

N

E

A

K
|
K

R

G

R

E

M

I

L

L

I

E

Q

K

T

I

C

A

G

K

Q

I

L

E

G

G

I

I

N

N

P

L

L

E

Q

D

A

T

I

V

A

A

M

V

G

G

D
|
D

G

G

A

A

N

N

D
|
D

L

I

P

S

M

M

M

F

G

K

A

K

A

A

G

G

L

L

S

K

V

I

A

A

Y

F

H

C

A

A

K

K

P

P

K

I

V

L

R

K

E

E

Q

K

A

A

M

D

V

I

A

C

I

I

N

E

A

K

G

R

G

D

L

L

D

R

R

E

L

I

L

L

E

K

L

Y

M

I

R

K

active site: D10 (= D30), F11 (≠ M31), D12 (= D32), G99 (= G121), K143 (= K169), D170 (= D196)
binding magnesium ion: D10 (= D30), D12 (= D32), D166 (= D192)
binding phosphate ion: D10 (= D30), F11 (≠ M31), D12 (= D32), S98 (= S120), G99 (= G121), K143 (= K169)

Q58989 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see 3 papers)
38% identity, 90% coverage: 25:236/236 of query aligns to 6:211/211 of Q58989

query
sites
Q58989

K

K

L

L

I

I

A

L

F

F

D
|
D

M

F

D
|
D

S

S

T

T

L

L

I

V

N

N

I

N

E
|
E

C

T

V

I

D

D

E

E

I

I

A

A

D

R

A

E

V

A

G

G

R

V

K

E

A

E

E

E

V

V

A

K

I

I

T

T

E

K

A

E

A

A

M

M

R

E

G

G

E

K

I

L

T

-

D

N

F

F

K

E

D

Q

S

S

L

L

R

R

R

K

R
|
R

V

V

A

S

L

L

K

K

G

D

V

L

S

P

M

I

A

E

S

K

M

V

D

E

E

K

V

A

Y

I

R

K

E

-

R

R

L

I

K

T

L

P

N

T

P

E

G

G

A

A

A

E

E

E

L

T

V

I

R

K

A

E

C

L

K

K

D

N

A

R

G

G

M

Y

K

V

I

V

L

A

L

V

V

V

S
|
S

G
|
G

G

G

F

F

T

D

Y

I

F

A

T

V

D

N

R

K

V

I

K

K

G

E

L

K

L

L

D

G

I

L

D

D

F

Y

T

A

R

F

S

A

N

N

V

R

L

L

E

I

V

V

R

K

D

D

G

G

L

K

L

L

T

T

G

G

K

D

M

V

I

-

D

-

Q

-

S

-

W

E

G

G

D

E

I

V

C

L

D

K

G

E

E

N

E

A

K
|
K

R

G

R

E

M

I

L

L

I

E

Q

K

T

I

C

A

G

K

Q

I

L

E

G

G

I

I

N

N

P

L

L

E

Q

D

A

T

I

V

A

A

M

V

G

G

D
|
D

G

G

A

A

N
|
N

D

D

L

I

P

S

M

M

M

F

G

K

A

K

A

A

G

G

L

L

S

K

V

I

A

A

Y

F

H

C

A

A

K

K

P

P

K

I

V

L

R

K

E

E

Q

K

A

A

M

D

V

I

A

C

I

I

N

E

A

K

G

R

G

D

L

L

D

R

R

E

L

I

L

L

E

K

L

Y

M

I

R

K

D11 (= D30) active site, Nucleophile; binding ; mutation to N: Loss of activity.
D13 (= D32) active site, Proton donor; binding
E20 (= E39) binding
R56 (= R76) binding
SG 99:100 (= SG 120:121) binding
K144 (= K169) binding
D167 (= D192) binding
N170 (= N195) binding

1l7nA Transition state analogue of phosphoserine phosphatase (aluminum fluoride complex) (see paper)
38% identity, 90% coverage: 25:236/236 of query aligns to 4:209/209 of 1l7nA

query
sites
1l7nA

K

K

L

L

I

I

A

L

F

F

D
|
D

M
x
F

D
|
D

S

S

T

T

L

L

I

V

N

N

I

N

E

E

C

T

V

I

D

D

E

E

I

I

A

A

D

R

A

E

V

A

G

G

R

V

K

E

A

E

E

E

V

V

A

K

I

I

T

T

E

K

A

E

A

A

M

M

R

E

G

G

E

K

I

L

T

-

D

N

F

F

K

E

D

Q

S

S

L

L

R

R

R

K

R

R

V

V

A

S

L

L

K

K

G

D

V

L

S

P

M

I

A

E

S

K

M

V

D

E

E

K

V

A

Y

I

R

K

E

-

R

R

L

I

K

T

L

P

N

T

P

E

G

G

A

A

A

E

E

E

L

T

V

I

R

K

A

E

C

L

K

K

D

N

A

R

G

G

M

Y

K

V

I

V

L

A

L

V

V

V

S
|
S

G
|
G

G

G

F

F

T

D

Y

I

F

A

T

V

D

N

R

K

V

I

K

K

G

E

L

K

L

L

D

G

I

L

D

D

F

Y

T

A

R

F

S

A

N

N

V

R

L

L

E

I

V

V

R

K

D

D

G

G

L

K

L

L

T

T

G

G

K

D

M

V

I

-

D

-

Q

-

S

-

W

E

G

G

D

E

I

V

C

L

D

K

G

E

E

N

E

A

K
|
K

R

G

R

E

M

I

L

L

I

E

Q

K

T

I

C

A

G

K

Q

I

L

E

G

G

I

I

N

N

P

L

L

E

Q

D

A

T

I

V

A

A

M

V

G

G

D
|
D

G

G

A

A

N
|
N

D
|
D

L

I

P

S

M

M

M

F

G

K

A

K

A

A

G

G

L

L

S

K

V

I

A

A

Y

F

H

C

A

A

K

K

P

P

K

I

V

L

R

K

E

E

Q

K

A

A

M

D

V

I

A

C

I

I

N

E

A

K

G

R

G

D

L

L

D

R

R

E

L

I

L

L

E

K

L

Y

M

I

R

K

active site: D9 (= D30), F10 (≠ M31), D11 (= D32), G98 (= G121), K142 (= K169), D169 (= D196)
binding aluminum fluoride: D9 (= D30), F10 (≠ M31), D11 (= D32), S97 (= S120), K142 (= K169)
binding tetrafluoroaluminate ion: D9 (= D30), F10 (≠ M31), D11 (= D32), S97 (= S120), G98 (= G121), K142 (= K169), N168 (= N195)
binding magnesium ion: D9 (= D30), D11 (= D32), D165 (= D192)

1l7pA Substrate bound phosphoserine phosphatase complex structure (see paper)
37% identity, 90% coverage: 25:236/236 of query aligns to 3:208/208 of 1l7pA

query
sites
1l7pA

K

K

L

L

I

I

A

L

F

F

D
x
N

M
x
F

D
|
D

S

S

T

T

L

L

I

V

N

N

I

N

E
|
E

C

T

V

I

D

D

E

E

I

I

A

A

D

R

A

E

V

A

G

G

R

V

K

E

A

E

E

E

V

V

A

K

I

I

T

T

E

K

A

E

A

A

M
|
M

R

E

G

G

E

K

I

L

T

-

D

N

F
|
F

K

E

D

Q

S

S

L

L

R

R

R

K

R
|
R

V

V

A

S

L

L

K

K

G

D

V

L

S

P

M

I

A

E

S

K

M

V

D

E

E

K

V

A

Y

I

R

K

E

-

R

R

L

I

K

T

L

P

N

T

P

E

G

G

A

A

A

E

E

E

L

T

V

I

R

K

A

E

C

L

K

K

D

N

A

R

G

G

M

Y

K

V

I

V

L

A

L

V

V

V

S
|
S

G
|
G

G

G

F

F

T

D

Y

I

F

A

T

V

D

N

R

K

V

I

K

K

G

E

L

K

L

L

D

G

I

L

D

D

F

Y

T

A

R

F

S

A

N

N

V

R

L

L

E

I

V

V

R

K

D

D

G

G

L

K

L

L

T

T

G

G

K

D

M

V

I

-

D

-

Q

-

S

-

W

E

G

G

D

E

I

V

C

L

D

K

G

E

E

N

E

A

K
|
K

R

G

R

E

M

I

L

L

I

E

Q

K

T

I

C

A

G

K

Q

I

L

E

G

G

I

I

N

N

P

L

L

E

Q

D

A

T

I

V

A

A

M

V

G

G

D

D

G

G

A

A

N

N

D
|
D

L

I

P

S

M

M

M

F

G

K

A

K

A

A

G

G

L

L

S

K

V

I

A

A

Y

F

H

C

A

A

K

K

P

P

K

I

V

L

R

K

E

E

Q

K

A

A

M

D

V

I

A

C

I

I

N

E

A

K

G

R

G

D

L

L

D

R

R

E

L

I

L

L

E

K

L

Y

M

I

R

K

active site: N8 (≠ D30), F9 (≠ M31), D10 (= D32), G97 (= G121), K141 (= K169), D168 (= D196)
binding phosphoserine: N8 (≠ D30), F9 (≠ M31), D10 (= D32), E17 (= E39), M40 (= M62), F46 (= F69), R53 (= R76), S96 (= S120), G97 (= G121), K141 (= K169)

1l7oA Crystal structure of phosphoserine phosphatase in apo form (see paper)
36% identity, 90% coverage: 25:236/236 of query aligns to 3:200/200 of 1l7oA

query
sites
1l7oA

K

K

L

L

I

I

A

L

F

F

D

N

M

F

D

D

S

S

T

T

L

L

I

V

N

N

I

N

E

E

C

T

V

I

D

D

E

E

I

I

A

A

D

R

A

E

V

A

G

G

R

V

K

E

A

E

E

E

V

V

A

K

A

-

I

-

T

-

E

-

A

-

M

-

R

-

G

-

E

K

I

I

T

T

D

N

F

F

K

E

D

Q

S

S

L

L

R

R

R

K

R

R

V

V

A

S

L

L

K

K

G

D

V

L

S

P

M

I

A

E

S

K

M

V

D

E

E

K

V

A

Y

I

R

K

E

-

R

R

L

I

K

T

L

P

N

T

P

E

G

G

A

A

A

E

E

E

L

T

V

I

R

K

A

E

C

L

K

K

D

N

A

R

G

G

M

Y

K

V

I

V

L

A

L

V

V

V

S

S

G

G

F

F

T

D

Y

I

F

A

T

V

D

N

R

K

V

I

K

K

G

E

L

K

L

L

D

G

I

L

D

D

F

Y

T

A

R

F

S

A

N

N

V

R

L

L

E

I

V

V

R

K

D

D

G

G

L

K

L

L

T

T

G

G

K

D

M

V

I

-

D

-

Q

-

S

-

W

E

G

G

D

E

I

V

C

L

D

K

G

E

E

N

E

A

K

K

R

G

R

E

M

I

L

L

I

E

Q

K

T

I

C

A

G

K

Q

I

L

E

G

G

I

I

N

N

P

L

L

E

Q

D

A

T

I

V

A

A

M

V

G

G

D

D

G

G

A

A

N

N

D

D

L

I

P

S

M

M

M

F

G

K

A

K

A

A

G

G

L

L

S

K

V

I

A

A

Y

F

H

C

A

A

K

K

P

P

K

I

V

L

R

K

E

E

Q

K

A

A

M

D

V

I

A
x
C

I

I

N
x
E

A

K

G

R

G

D

L

L

D

R

R

E

L

I

L

L

E

K

L

Y

M

I

R

K

binding zinc ion: C186 (≠ A222), E188 (≠ N224)

3m1yC Crystal structure of a phosphoserine phosphatase (serb) from helicobacter pylori
39% identity, 85% coverage: 25:224/236 of query aligns to 4:196/208 of 3m1yC

query
sites
3m1yC

K

K

L

L

I

A

A

V

F

F

D
|
D

M
x
F

D
|
D

S

S

T

T

L

L

I

V

N

N

I

A

E

E

C

T

V

I

D

E

E

S

I

L

A

A

D

R

A

A

V

W

G

G

R

V

K

F

A

D

E

E

V

V

A

K

A

T

I

I

T

T

E

L

A

K

A

A

M

M

R

N

G

G

E

-

I

-

T

T

D

D

F

F

K

H

D

K

S

S

L

L

R

I

R

L

R

R

V

V

A

S

L

K

L

L

K

K

G

N

V

M

S

P

M

L

A

K

S

L

M

A

D

K

E

E

V

V

Y

C

R

-

E

E

R

S

L

L

K

P

L

L

N

F

P

E

G

G

A

A

A

L

E

E

L

L

V

V

R

S

A

A

C

L

K

K

D

E

A

K

G

N

M

Y

K

K

I

V

L

V

L

C

V

F

S

S

G
|
G

G

G

F

F

T

D

Y

L

F

A

T

T

D

N

R

H

V

Y

K

R

G

D

L

L

D

H

I

L

D

D

F

A

T

A

R

F

S

S

N

N

V

T

L

L

E

I

V

V

R

E

-

N

-

D

-

A

-

L

D

N

G

G

L

L

L

V

T

T

G

G

K

H

M

M

I

M

D

-

Q

-

S

-

W

-

G

-

D

-

I

-

C

-

D

F

G

S

E

H

E

S

K
|
K

R

G

R

E

M

M

L

L

I

L

Q

V

T

L

C

Q

G

R

Q

L

L

L

G

N

I

I

N

S

P

K

L

T

Q

N

A

T

I

L

A

V

M

V

G

G

D
|
D

G

G

A

A

N

N

D
|
D

L

L

P

S

M

M

M

F

G

K

A

H

A

A

G

H

L

I

S

K

V

I

A

A

Y

F

H

N

A

A

K

K

P

E

K

V

V

L

R

K

E

Q

Q

H

A

A

M

T

V

H

A

C

I

I

N

N

active site: D9 (= D30), F10 (≠ M31), D11 (= D32), G97 (= G121), K141 (= K169), D168 (= D196)
binding magnesium ion: D9 (= D30), D11 (= D32), D164 (= D192)

3kd3A Crystal structure of a phosphoserine phosphohydrolase-like protein from francisella tularensis subsp. Tularensis schu s4
32% identity, 78% coverage: 25:209/236 of query aligns to 2:188/216 of 3kd3A

query
sites
3kd3A

K

K

L

N

I

I

A

I

F

F

D
|
D

M
x
F

D
|
D

S

S

T

T

L

L

I

I

N

K

I

K

E

E

C

S

V

L

D

E

E

L

I

I

A

L

D

E

A

P

V

I

G

L

R

Q

K

K

A

S

-

P

-

A

-

K

-

L

-

K

E

E

V

I

A

E

A

Y

I

I

T

T

E

N

A

L

A

G

M

M

R

Q

G

G

E

D

I

I

T

S

D

-

F

F

K

R

D

D

S

S

L

L

R

Q

R

K

R

R

V

L

A

A

L

I

L

A

K

S

G

P

V

T

-

K

-

Q

S

S

M

I

A

K

S

E

M

F

D

S

E

N

V

K

Y

Y

R

C

E

P

R

N

L

L

K

-

L

L

N

T

P

D

G

G

A

I

A

K

E

E

L

L

V

V

R

Q

A

D

C

L

K

K

D

N

A

K

G

G

M

F

K

E

I

I

L

W

L

I

V

F

S

S

G
|
G

G

G

F

L

T

S

Y

E

F

S

T

I

D

Q

R

P

V

F

K

A

G

D

L

Y

L

L

D

N

I

I

D

-

F

-

T

P

R

R

S

E

N

N

V

I

L

F

E

A

V

V

R

E

-

T

-

I

-

W

-

N

-

S

D

D

G

G

L

-

T

S

G

F

K

K

M

E

I

L

D

D

Q

N

S

S

W

N

G

G

D

-

I

A

C

C

D

D

G

S

E

K

-

L

-

S

-

A

-

F

E

D

K

K

R

A

R

K

M

G

L

L

I

I

Q

D

T

G

C

-

G

-

Q

-

L

-

G

-

I

-

N

-

P

-

L

-

Q

E

A

V

I

I

A

A

M

I

G

G

D
|
D

G

G

A

Y

N

T

D
|
D

L

Y

P

Q

M

L

M

Y

-

E

-

K

G

G

A

Y

A

A

G

T

L

K

S

F

V

I

A

A

Y

Y

active site: D7 (= D30), F8 (≠ M31), D9 (= D32), G103 (= G121), D173 (= D196)
binding magnesium ion: D7 (= D30), D9 (= D32), D169 (= D192)

6hyjB Psph human phosphoserine phosphatase (see paper)
33% identity, 48% coverage: 27:139/236 of query aligns to 17:128/223 of 6hyjB

query
sites
6hyjB

I

V

A

C

F

F

D
|
D

M

V

D
|
D

S

S

T

T

L

V

I

I

N

R

I

E

E
|
E

C

G

V

I

D

D

E

E

I

L

A

A

D

K

A

I

V

C

G

G

R

V

K

E

A

D

E

A

V

V

A

S

A

E

I

M

T

T

E

R

A

R

A

A

M

M

R

G

G

G

E

A

I

V

T

P

D

-

F

F

K

K

D

A

S

A

L

L

R

T

R

E

R

R

V

L

A

A

L

L

L

I

K

Q

G

P

V

S

S

R

M

E

A

Q

S

V

M

Q

D

R

E

L

V

I

Y

A

R

E

E

Q

R

P

L

P

K

H

L

L

N

T

P

P

G

G

A

I

A

R

E

E

L

L

V

V

R

S

A

R

C

L

K

Q

D

E

A

R

G

N

M

V

K

Q

I

V

L

F

L

L

V

I

S

S

G

G

F

F

T

R

Y

S

F

I

T

V

D

E

R

H

V

V

K

A

G

S

L

K

L

L

D

N

I

I

D

P

F

A

T

T

binding calcium ion: D20 (= D30), D22 (= D32)
binding serine: E29 (= E39)

Sites not aligning to the query:

6q6jB Human phosphoserine phosphatase with substrate analogue homo-cysteic acid (see paper)
33% identity, 48% coverage: 27:139/236 of query aligns to 13:124/217 of 6q6jB

query
sites
6q6jB

I

V

A

C

F

F

D
|
D

M
x
V

D
|
D

S

S

T

T

L

V

I

I

N

R

I

E

E

E

C

G

V

I

D

D

E

E

I

L

A

A

D

K

A

I

V

C

G

G

R

V

K

E

A

D

E

A

V

V

A

S

A

E

I

M

T

T

E

R

A

R

A

A

M

M

R

G

G

G

E

A

I

V

T

P

D

-

F
|
F

K

K

D

A

S

A

L

L

R

T

R

E

R

R

V

L

A

A

L

L

L

I

K

Q

G

P

V

S

S

R

M

E

A

Q

S

V

M

Q

D

R

E

L

V

I

Y

A

R

E

E

Q

R

P

L

P

K

H

L

L

N

T

P

P

G

G

A

I

A

R

E

E

L

L

V

V

R

S

A

R

C

L

K

Q

D

E

A

R

G

N

M

V

K

Q

I

V

L

F

L

L

V

I

S
|
S

G
|
G

F

F

T

R

Y

S

F

I

T

V

D

E

R

H

V

V

K

A

G

S

L

K

L

L

D

N

I

I

D

P

F

A

T

T

binding calcium ion: D16 (= D30), D18 (= D32)
binding (2~{S})-2-azanyl-4-sulfo-butanoic acid: D16 (= D30), V17 (≠ M31), D18 (= D32), F54 (= F69), S105 (= S120), G106 (= G121), G107 (= G122)

Sites not aligning to the query:

P78330 Phosphoserine phosphatase; PSP; PSPase; L-3-phosphoserine phosphatase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Homo sapiens (Human) (see 4 papers)
33% identity, 48% coverage: 27:139/236 of query aligns to 17:128/225 of P78330

query
sites
P78330

I

V

A

C

F

F

D
|
D

M
x
V

D
|
D

S
|
S

T

T

L

V

I

I

N

R

I

E

E
|
E

C

G

V

I

D
|
D

E

E

I

L

A
|
A

D

K

A

I

V

C

G

G

R

V

K

E

A

D

E

A

V

V

A

S

A

E

I

M

T

T

E

R

A

R

A

A

M
|
M

R
x
G

G

G

E

A

I

V

T

P

D

-

F

F

K

K

D

A

S

A

L

L

R

T

R

E

R
|
R

V

L

A

A

L

L

L

I

K

Q

G

P

V

S

S

R

M

E

A

Q

S

V

M

Q

D

R

E

L

V

I

Y

A

R

E

E

Q

R

P

L

P

K

H

L

L

N

T

P

P

G

G

A

I

A

R

E

E

L

L

V

V

R

S

A

R

C

L

K

Q

D

E

A

R

G

N

M

V

K

Q

I

V

L

F

L

L

V

I

S
|
S

G
|
G

F

F

T

R

Y

S

F

I

T

V

D

E

R

H

V

V

K

A

G

S

L

K

L

L

D

N

I

I

D

P

F

A

T

T

D20 (= D30) binding
DVD 20:22 (≠ DMD 30:32) binding
D22 (= D32) binding
S23 (= S33) mutation to A: Reduces L-phosphoserine phosphatase activity by about 50%.; mutation to T: Reduces L-phosphoserine phosphatase activity by about 80%.
E29 (= E39) mutation to D: Reduces L-phosphoserine phosphatase activity by about 95%.; mutation to Q: Loss of L-phosphoserine phosphatase activity.
D32 (= D42) to N: in PSPHD; decreased L-phosphoserine phosphatase activity; dbSNP:rs104894035
A35 (= A45) to T: in PSPHD; decreased L-phosphoserine phosphatase activity
M52 (= M62) binding ; to T: in PSPHD; decreased L-phosphoserine phosphatase activity; dbSNP:rs104894036
G53 (≠ R63) binding
R65 (= R76) mutation R->A,K: Loss of L-phosphoserine phosphatase activity.
SGG 109:111 (= SGG 120:122) binding ; binding

Sites not aligning to the query:

133 N→A: Reduces L-phosphoserine phosphatase activity by about 75%.
158 binding ; binding
179 binding
182 binding ; binding ; T→S: Reduces L-phosphoserine phosphatase activity by about 99%.; T→V: Reduces L-phosphoserine phosphatase activity by about 25%.
202 R→A: Reduces L-phosphoserine phosphatase activity by about 99%.; R→K: Reduces L-phosphoserine phosphatase activity by about 95%.

1l8oA Molecular basis for the local conformational rearrangement of human phosphoserine phosphatase (see paper)
33% identity, 48% coverage: 27:139/236 of query aligns to 14:125/222 of 1l8oA

query
sites
1l8oA

I

V

A

C

F

F

D
|
D

M
x
V

D
|
D

S

S

T

T

L

V

I

I

N

R

I

E

E

E

C

G

V

I

D

D

E

E

I

L

A

A

D

K

A

I

V

C

G

G

R

V

K

E

A

D

E

A

V

V

A

S

A

E

I

M

T

T

E

R

A

R

A

A

M

M

R

G

G

G

E

A

I

V

T

P

D

-

F

F

K

K

D

A

S

A

L

L

R

T

R

E

R

R

V

L

A

A

L

L

L

I

K

Q

G

P

V

S

S

R

M

E

A

Q

S

V

M

Q

D

R

E

L

V

I

Y

A

R

E

E

Q

R

P

L

P

K

H

L

L

N

T

P

P

G

G

A

I

A

R

E

E

L

L

V

V

R

S

A

R

C

L

K

Q

D

E

A

R

G

N

M

V

K

Q

I

V

L

F

L

L

V

I

S
|
S

G
|
G

G

G

F

F

T

R

Y

S

F

I

T

V

D

E

R

H

V

V

K

A

G

S

L

K

L

L

D

N

I

I

D

P

F

A

T

T

active site: D17 (= D30), V18 (≠ M31), D19 (= D32), G107 (= G121)
binding phosphate ion: D17 (= D30), D19 (= D32), S106 (= S120)

Sites not aligning to the query:

1l8lA Molecular basis for the local confomational rearrangement of human phosphoserine phosphatase (see paper)
33% identity, 48% coverage: 27:139/236 of query aligns to 14:125/222 of 1l8lA

query
sites
1l8lA

I

V

A

C

F

F

D
|
D

M
x
V

D
|
D

S

S

T

T

L

V

I

I

N

R

I

E

E

E

C

G

V

I

D

D

E

E

I

L

A

A

D

K

A

I

V

C

G

G

R

V

K

E

A

D

E

A

V

V

A

S

A

E

I

M

T

T

E

R

A

R

A

A

M

M

R

G

G

G

E

A

I

V

T

P

D

-

F

F

K

K

D

A

S

A

L

L

R

T

R

E

R

R

V

L

A

A

L

L

L

I

K

Q

G

P

V

S

S

R

M

E

A

Q

S

V

M

Q

D

R

E

L

V

I

Y

A

R

E

E

Q

R

P

L

P

K

H

L

L

N

T

P

P

G

G

A

I

A

R

E

E

L

L

V

V

R

S

A

R

C

L

K

Q

D

E

A

R

G

N

M

V

K

Q

I

V

L

F

L

L

V

I

S

S

G
|
G

G

G

F

F

T

R

Y

S

F

I

T

V

D

E

R

H

V

V

K

A

G

S

L

K

L

L

D

N

I

I

D

P

F

A

T

T

active site: D17 (= D30), V18 (≠ M31), D19 (= D32), G107 (= G121)
binding d-2-amino-3-phosphono-propionic acid: D17 (= D30), D19 (= D32), G107 (= G121)

Sites not aligning to the query:

active site: 155, 180
binding d-2-amino-3-phosphono-propionic acid: 155, 176, 177, 179

6hyyA Human phosphoserine phosphatase with serine and phosphate (see paper)
33% identity, 48% coverage: 27:139/236 of query aligns to 13:124/221 of 6hyyA

query
sites
6hyyA

I

V

A

C

F

F

D
|
D

M
x
V

D
|
D

S

S

T

T

L

V

I

I

N

R

I

E

E

E

C

G

V

I

D

D

E

E

I

L

A

A

D

K

A

I

V

C

G

G

R

V

K

E

A

D

E

A

V

V

A

S

A

E

I

M

T

T

E

R

A

R

A

A

M

M

R

G

G

G

E

A

I

V

T

P

D

-

F

F

K

K

D

A

S

A

L

L

R

T

R

E

R

R

V

L

A

A

L

L

L

I

K

Q

G

P

V

S

S

R

M

E

A

Q

S

V

M

Q

D

R

E

L

V

I

Y

A

R

E

E

Q

R

P

L

P

K

H

L

L

N

T

P

P

G

G

A

I

A

R

E

E

L

L

V

V

R

S

A

R

C

L

K

Q

D

E

A

R

G

N

M

V

K

Q

I

V

L

F

L

L

V

I

S
|
S

G
|
G

G

G

F

F

T

R

Y

S

F

I

T

V

D

E

R

H

V

V

K

A

G

S

L

K

L

L

D

N

I

I

D

P

F

A

T

T

binding magnesium ion: D16 (= D30), D18 (= D32)
binding serine: D16 (= D30), V17 (≠ M31), D18 (= D32), S105 (= S120), G106 (= G121)

Sites not aligning to the query:

4ap9A Crystal structure of phosphoserine phosphatase from t.Onnurineus in complex with ndsb-201 (see paper)
27% identity, 78% coverage: 25:208/236 of query aligns to 10:174/200 of 4ap9A

query
sites
4ap9A

K

K

L

V

I

A

A

V

F

I

D
|
D

M
x
I

D
x
E

S

G

T

T

L

L

I

T

N

D

I

F

E

E

C

F

V

W

D

R

E

E

I

M

A

A

D
x
R

A
x
I

V
x
T

G

G

R

K

K

R

A

-

E

E

V

I

A

E

I

L

T

L

E

E

A

K

A

G

M
x
L

R

S

G

G

E

E

I

V

T

-

D

E

F
x
W

K

L

D

D

S

S

L

L

R

L

R

K

R

R

V

V

A

G

L

L

L

I

K

R

G

G

V

I

S

D

M

E

A

G

S

T

M

F

D

L

E

R

V

T

Y

-

R

R

E

E

R

K

L

V

K

N

L

V

N

S

P

P

G

E

A

A

A

R

E

E

L

L

V

V

R

E

A

T

C

L

K

R

D

E

A

K

G

G

M

F

K

K

I

V

L

V

L

L

V

I

S

S

G
|
G

G

S

F

F

T

E

Y

E

F

V

T

L

D

E

R

P

V

F

K

K

G

E

L

L

L

G

D

D

I

E

D

F

F

M

T

A

R

N

S

R

N

A

V

I

L

F

E

E

V

-

R

-

D

D

G

G

L

K

L

F

T

Q

G

G

K

I

M

-

I

-

D

-

Q

-

S

-

W

-

G

-

D

-

I

-

C

-

D

-

G

-

E

-

K

-

R

-

R

R

M

L

L

R

I

F

Q

R

T
x
D

C
x
K

G

G

Q

E

L

F

-

L

-

K

G

R

I

F

N

R

P

D

L

G

Q

F

A

I

I

L

A

A

M

M

G

G

D

D

G

G

A
x
Y

N
x
A

D
|
D

L

A

P

K

M

M

M

F

G

E

A

R

A

A

G

D

L

M

S

G

V

I

A

A

active site: D15 (= D30), I16 (≠ M31), E17 (≠ D32), G103 (= G121), K141 (≠ C177), D162 (= D196)
binding 3-pyridinium-1-ylpropane-1-sulfonate: R31 (≠ D46), I32 (≠ A47), T33 (≠ V48), L46 (≠ M62), W52 (≠ F69), D140 (≠ T176), K141 (≠ C177), Y160 (≠ A194), A161 (≠ N195)

Query Sequence

>WP_011801935.1 NCBI__GCF_000015505.1:WP_011801935.1
MNPIEHSPGLVVNIATPDLKLRDFKLIAFDMDSTLINIECVDEIADAVGRKAEVAAITEA
AMRGEITDFKDSLRRRVALLKGVSMASMDEVYRERLKLNPGAAELVRACKDAGMKILLVS
GGFTYFTDRVKGLLDIDFTRSNVLEVRDGLLTGKMIDQSWGDICDGEEKRRMLIQTCGQL
GINPLQAIAMGDGANDLPMMGAAGLSVAYHAKPKVREQAMVAINAGGLDRLLELMR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory