SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011802240.1 NCBI__GCF_000015505.1:WP_011802240.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P78061 Gamma-glutamylputrescine synthetase PuuA; Gamma-Glu-Put synthetase; Glutamate--putrescine ligase; EC 6.3.1.11 from Escherichia coli (strain K12) (see paper)
34% identity, 98% coverage: 9:456/459 of query aligns to 19:469/472 of P78061

query
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P78061

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H282 (= H269) mutation to N: Activity is impaired to 9% of wild-type.
R357 (= R345) mutation to Q: Activity is impaired to 3% of wild-type.

8ufjB Glutamine synthetase (see paper)
32% identity, 99% coverage: 7:459/459 of query aligns to 1:444/444 of 8ufjB

query
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8ufjB

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binding magnesium ion: E132 (= E148), E134 (= E150), E189 (= E213), E196 (= E220), H245 (= H269), E333 (= E357)

8tfkA Glutamine synthetase (see paper)
34% identity, 90% coverage: 46:459/459 of query aligns to 19:440/440 of 8tfkA

query
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8tfkA

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P

P

E

T

M

N

K

V

G

N

D

I

A

F

Y

H

L

L

S

S

P

D

Y

K

-

E

-

R

-

E

-

R

-

G

-

I

-

R

S

S

L

L

P

P

R

A

S

D

L

L

G

K

E

E

A

A

L

I

D

D

W

E

L

M

R

K

R

G

E

S

S

K

D

F

L

V

H

K

E

E

V

A

L

L

G

G

R

E

E

H

F

V

V

F

T

S

V

H

Y

Y

S

L

E

C

I

A

K

K

E

E

M

M

E

E

F

W

A

D

E

E

F

Y

M

K

K

A

V

V

I

V

S

H

P

P

W

W

E

E

R

L

E

S

H

R

L

Y

L

L

L

S

L

M

V

L

binding adenosine-5'-diphosphate: E128 (= E148), D194 (≠ N222), F195 (= F223), F197 (≠ H225), N243 (≠ H271), R312 (= R340), R317 (= R345), G325 (≠ A353), R327 (= R355)
binding magnesium ion: E128 (= E148), E128 (= E148), E130 (= E150), E185 (= E213), E192 (= E220), E192 (= E220), H241 (= H269), E329 (= E357)
binding l-methionine-s-sulfoximine phosphate: E128 (= E148), E130 (= E150), E185 (= E213), E192 (= E220), G237 (= G265), H241 (= H269), R294 (= R322), E300 (≠ A328), R312 (= R340), R331 (= R359)

8ooqB Glutamine synthetase from Methanothermococcus thermolithotrophicus (see paper)
33% identity, 83% coverage: 72:451/459 of query aligns to 50:439/446 of 8ooqB

query
sites
8ooqB

E

E

S

N

G

G

P

L

Y

Y

Y

F

D

D

-

G

-

S

-

I

-

E

-

G

V

F

I

V

S

G

P

I

T

N

D

E

K

S

D

D

M

M

H

M

L

L

R

K

P

P

D

D

P

L

S

S

T

T

V

F

R

S

I

V

V

L

P

P

W

W

-
x
R

-

P

A

S

S

E

N

K

P

S

T
x
V

A

A

Q

R

V

V

I

I

H

C

D

D

C

V

F

Y

D

T

P

T

A

K

G

G

K

K

L

P

I

F

P

E

F

G

A

D

P

P

R

R

S

G

V

C

L

L

R

K

R

R

V

V

C

M

E

E

L

E

Y

F

E

K

A

K

E

E

-

F

G

N

L

G

V

E

P

Y

V

F

V

V

A

G

P

P

E

E

L

P

E
|
E

F

F

Y

F

L

L

V

L

A

K

R

K

N

D

-

P

T

H

D

N

P

P

N

H

T

K

L

Y

L

I

R

-

P

-

P

P

I

A

G

D

R

D

S

G

G

G

R

Y

A

-

E

-

T

-

S

-

R

-

Q

-

A

-

Y

F

S

D

I

L

D

E

A

P

V

M

N

D

E

E

F

A

D
x
P

P

D

L

I

F
x
R

E
x
R

D

D

I

I

Y

V

D

F

Y

A

C

L

E

E

K

N

M

L

E

G

L

F

N

H

V

V

D

E

T

A

L
x
S

I

H

H

H

E
|
E

V

V

G

A

A

P

G

G

Q

Q

M

H

E
|
E

I

V

N

D

F

F

F

K

H

F

A

D

H

D

P

A

L

L

G

K

L

T

A

A

D

D

E

S

V

V

F

I

F

T

F

F

K

K

R

T

T

T

V

I

R

K

E

T

A

I

A

A

L

E

R

Q

H

H

N

G

M

L

Y

K

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

F

A

F

G

G

E

M

P

N

G

G

S

S

A

G

M

M

H

H

V

C

H

H

Q

Q

S

S

I

I

L

W

S

L

K

N

E

G

T

E

G

P

L

S

N

F

I

Y

F

D

S

E

N

N

P

A

D

P

G

Y

S

Q

E

L

S

S

E

E

A

T

F

C

F

M

H

N

Y

Y

I

V

G

A

G

G

L

I

Q

L

R

K

Y

H

I

A

P

K

A

A

A

I

M

V

A

A

L

I

V

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

S

V

R

P

D

G

T

Y

A

E

A

A

P

P

I

V

N

N

I

I

A

A

W

W

G

A

H

N

D

S

N

N

R

R

T

S

V

A

G

I

I

I

R

R

S

V

P

P

I

A

A

A

T

R

P

G

Q

K

A

G

R

T

R

R

V

I

E

E

N

F

R

R

V

A

I

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

V

L

A

A

L

F

A

T

M

V

T

M

L

L

A

A

C

A

G

G

Y

L

L

D

G

G

I

V

K

K

N

N

K

K

I

L

K

D

-

A

P

P

K

E

P

P

E

V

M

E

K

R

G

N

-

I

-

F

-

A

-

M

-

S

-

E

-

A

-

E

D

K

A

K

Y

E

L

L

S

G

P

I

Y

E

S

S

L

V

P

P

R

A

S

N

L

L

G

K

E

A

A

A

L

L

D

D

W

E

L

L

R

E

R

N

E

N

S

D

D

V

L

L

H

K

E

N

V

A

L

L

G

G

R

K

E

H

F

I

V

F

T

E

V

S

Y

F

S

L

E

E

I

I

K

K

E

N

M

A

E

E

F

W

A

D

E

S

F

F

M

R

K

T

V

S

I

V

S

T

P

D

W

W

E

E

binding 2-oxoglutaric acid: R86 (vs. gap), V92 (≠ T106), P169 (≠ D190), R172 (≠ F193), R173 (≠ E194), S189 (≠ L210)
binding magnesium ion: E137 (= E150), E192 (= E213), E199 (= E220)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 16, 18, 32

8oooA Glutamine synthetase from methanothermococcus thermolithotrophicus in complex with 2-oxoglutarate and mgatp at 2.15 a resolution (see paper)
33% identity, 83% coverage: 72:451/459 of query aligns to 51:440/447 of 8oooA

query
sites
8oooA

E

E

S

N

G

G

P

L

Y

Y

Y

F

D

D

-

G

-

S

-

I

-

E

-

G

V

F

I

V

S

G

P

I

T

N

D

E

K

S

D

D

M

M

H

M

L

L

R

K

P

P

D

D

P

L

S

S

T

T

V

F

R

S

I

V

V

L

P

P

W

W

-
x
R

-

P

A

S

S

E

N

K

P

S

T
x
V

A

A

Q

R

V

V

I

I

H

C

D

D

C

V

F

Y

D

T

P

T

A

K

G

G

K

K

L

P

I

F

P

E

F

G

A

D

P

P

R

R

S

G

V

C

L

L

R

K

R

R

V

V

C

M

E

E

L

E

Y

F

E

K

A

K

E

E

-

F

G

N

L

G

V

E

P

Y

V

F

V

V

A

G

P

P

E
|
E

L

P

E

E

F

F

Y

F

L

L

V

L

A

K

R

K

N

D

-

P

T

H

D

N

P

P

N

H

T

K

L

Y

L

I

R

-

P

-

P

P

I

A

G

D

R

D

S

G

G

G

R

Y

A

-

E

-

T

-

S

-

R

-

Q

-

A

-

Y

F

S

D

I

L

D

E

A

P

V

M

N

D

E

E

F

A

D
x
P

P

D

L

I

F
x
R

E
x
R

D

D

I

I

Y

V

D

F

Y

A

C

L

E

E

K

N

M

L

E

G

L

F

N

H

V

V

D
x
E

T

A

L
x
S

I

H

H

H

E

E

V

V

G

A

A

P

G

G

Q

Q

M

H

E

E

I

V

N

D

F
|
F

F
x
K

H
x
F

A

D

H

D

P

A

L

L

G

K

L

T

A

A

D

D

E

S

V

V

F

I

F

T

F

F

K

K

R

T

T

T

V

I

R

K

E

T

A

I

A

A

L

E

R

Q

H

H

N

G

M

L

Y

K

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

F

A

F

G

G

E

M

P

N

G

G

S

S

A

G

M

M

H

H

V

C

H
|
H

Q

Q

S
|
S

I

I

L

W

S

L

K

N

E

G

T

E

G

P

L

S

N

F

I

Y

F

D

S

E

N

N

P

A

D

P

G

Y

S

Q

E

L

S

S

E

E

A

T

F

C

F

M

H

N

Y

Y

I

V

G

A

G

G

L

I

Q

L

R

K

Y

H

I

A

P

K

A

A

A

I

M

V

A

A

L

I

V

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

S

V

R

P

D

G

T

Y

A

E

A

A

P

P

I

V

N

N

I

I

A

A

W

W

G

A

H

N

D

S

N

N

R

R

T

S

V

A

G

I

I

I

R
|
R

S

V

P

P

I

A

A

A

T

R

P

G

Q

K

A

G

R

T

R
|
R

V

I

E

E

N

F

R

R

V

A

I

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

V

L

A

A

L

F

A

T

M

V

T

M

L

L

A

A

C

A

G

G

Y

L

L

D

G

G

I

V

K

K

N

N

K

K

I

L

K

D

-

A

P

P

K

E

P

P

E

V

M

E

K

R

G

N

-

I

-

F

-

A

-

M

-

S

-

E

-

A

-

E

D

K

A

K

Y

E

L

L

S

G

P

I

Y

E

S

S

L

V

P

P

R

A

S

N

L

L

G

K

E

A

A

A

L

L

D

D

W

E

L

L

R

E

R

N

E

N

S

D

D

V

L

L

H

K

E

N

V

A

L

L

G

G

R

K

E

H

F

I

V

F

T

E

V

S

Y

F

S

L

E

E

I

I

K

K

E

N

M

A

E

E

F

W

A

D

E

S

F

F

M

R

K

T

V

S

I

V

S

T

P

D

W

W

E

E

binding 2-oxoglutaric acid: R87 (vs. gap), V93 (≠ T106), P170 (≠ D190), R173 (≠ F193), R174 (≠ E194), S190 (≠ L210)
binding adenosine-5'-triphosphate: E136 (= E148), E188 (≠ D208), F203 (= F223), K204 (≠ F224), F205 (≠ H225), H251 (= H271), S253 (= S273), R325 (= R345), R335 (= R355)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 17, 19, 33

8oozA Glutamine synthetase (see paper)
31% identity, 95% coverage: 20:456/459 of query aligns to 10:427/430 of 8oozA

query
sites
8oozA

L

V

N

E

E

E

R

K

R

N

V

V

T

R

E

F

V

V

E

R

C

L

L

Q

V

F

P

V

D

D

L

V

T

Q

G

G

V

I

A

P

R

K

G

N

K

V

I

A

L

I

P

P

R

V

G

G

K

Q

F

L

T

E

E

K

D

A

R

L

G

G

M

-

R

-

L

-

P

-

E

-

A

-

V

-

A

-

M

-

G

-

V

-

T

-

G

-

E

-

F

-

P

-

E

-

S

P

G

G

P

I

Y

H

Y

F

D

D

V

G

I

S

S

S

P

I

T

E

D

G

K

S

D

D

M

M

H

V

L

L

R

R

P

P

D

D

P

P

S

D

T

T

V

F

R

R

I

V

V

L

P

P

W

W

A

-

S

S

N

T

P

A

T

E

A

A

Q

R

V

L

I

I

H

C

D

D

C

I

F

E

D

L

P

P

A

D

G

G

K

K

L

P

I

F

P

M

F

G

A

C

P

P

R

R

S

Q

V

V

L

L

R

K

V

N

C

M

E

E

L

E

Y

A

E

A

A

K

E

L

G

G

L

Y

V

V

P

M

V

N

V

T

A
x
G

P

P

E
|
E

L

M

E

E

F

F

Y

F

L

L

V

F

A

K

R

R

N

Q

T

D

D

G

P

M

N

P

T

T

L

N

L

I

R

-

P

-

P

P

I

Q

G

D

R

R

S

G

G

G

R

Y

A

F

E

D

T

L

S

A

R

-

Q

-

A

-

Y

-

S

P

I

I

D

D

A

L

V

A

N

E

E

E

F

I

D

K

P

-

L

-

F

-

E

R

D

E

I

I

Y

V

D

L

Y

V

C

L

E

E

K

E

M

M

E

G

L

F

N

E

V

V

D
x
E

T

A

L

A

I

H

H

H

E

E

V

V

G

A

A

F

G

G

Q

Q

M

H

E

E

I

I

N

D

F
|
F

F
x
K

H
x
Y

A

D

H

N

P

A

L

L

G

A

L

T

A

A

D

D

E

N

V

V

F

I

F

T

F

L

K

K

R

Y

T

V

V

A

R

K

E

T

A

L

A

A

L

L

R

Q

H

H

N

G

M

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

I

A

F

G

G

E

V

P

N

G

G

S

S

A

G

M

M

H
|
H

V

T

H
x
N

Q

T

S
|
S

I

L

L

F

S

-

K

-

E

K

T

D

G

G

L

K

N

N

I

A

F

F

S

Y

N

D

P

P

D

D

G

A

S

P

E

D

-

Q

-

I

S

S

E

D

A

T

F

L

F

R

H

Y

Y

F

I

V

G

G

L

V

Q

L

R

K

Y

H

I

I

P

R

A

A

A

I

M

T

A

A

L

I

V

T

A

N

P

P

Y

L

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

S

V

R

P

D

G

T

Y

A

E

A

A

P

P

I

V

N

Y

I

I

A

T

W

W

G

S

H

G

D

P

N

N

R

R

T

S

V

S

G

L

I

I

R

R

S

V

P

P

I

A

A

P

T

R

P

G

Q

N

A
x
S

R

T

R
|
R

V

I

E
|
E

N

I

R

R

V

S

I

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

V

L

A

A

L

F

A

A

M

A

T

I

L

L

A

A

C

A

G

G

Y

L

L

D

G

G

I

V

K

K

N

N

K

K

I

I

K

E

P

P

K

P

P

E

E

R

M

V

K

E

G

K

D

N

A

I

Y

Y

-

K

-

L

-

T

-

E

-

R

-

E

-

K

L

L

S

G

P

I

Y

G

S

M

L

L

P

P

R

G

S

T

L

L

G

K

E

E

A

A

L

I

D

E

W

C

L

F

R

K

R

E

E

D

S

E

D

L

L

L

H

V

E

S

V

A

L

L

G

G

R

E

E

H

F

V

V

S

T

Q

V

S

Y

I

S

I

E

N

I

V

K

A

E

M

M

A

E

D

F

W

A

D

E

S

F

Y

M

R

K

T

V

Q

I

V

S

H

P

Q

W

W

E

E

R

L

E

D

H

R

L

Y

L

L

binding adenosine-5'-triphosphate: G117 (≠ A146), E170 (≠ D208), F185 (= F223), K186 (≠ F224), Y187 (≠ H225), N233 (≠ H271), S235 (= S273), S315 (≠ A353), R317 (= R355)
binding magnesium ion: E119 (= E148), H231 (= H269), E319 (= E357)

8ooxB Glutamine synthetase (see paper)
31% identity, 95% coverage: 20:456/459 of query aligns to 10:435/438 of 8ooxB

query
sites
8ooxB

L

V

N

E

E

E

R

K

R

N

V

V

T

R

E

F

V

V

E

R

C

L

L

Q

V

F

P

V

D

D

L

V

T

Q

G

G

V

I

A

P

R

K

G

N

K

V

I

A

L

I

P

P

R

V

G

G

K

Q

F

L

T

E

E

K

D

-

R

-

G

-

M

-

R

-

L

-

P

-

E

-

A

-

V

-

A

A

M

L

G

G

V

P

T

G

G

I

E

H

F

F

P

-

E

D

S

G

G

S

P

S

Y

I

Y

E

D

G

V

F

I

V

S

R

P

I

T

E

D

E

K

S

D

D

M

M

H

V

L

L

R

R

P

P

D

D

P

P

S

D

T

T

V

F

R

R

I

V

V

L

P

P

W

W

A

S

S

G

N

N

P

T

T

A

-

E

A

A

Q

R

V

L

I

I

H

C

D

D

C

I

F

E

D

L

P

P

A

D

G

G

K

K

L

P

I

F

P

M

F

G

A

C

P

P

R

R

S

Q

V

V

L

L

R

K

V

N

C

M

E

E

L

E

Y

A

E

A

A

K

E

L

G

G

L

Y

V

V

P

M

V

N

V

T

A

G

P

P

E

E

L

M

E
|
E

F

F

Y

F

L

L

V

F

A

K

R

R

N

Q

T

D

D

G

P

M

N

P

T

T

L

N

L

I

R

-

P

-

P

P

I

Q

G

D

R

R

S

G

G

G

R

Y

A

F

E

D

T

L

S

A

R

-

Q

-

A

-

Y

-

S

P

I

I

D

D

A

L

V

A

N

E

E

E

F

I

D

K

P

-

L

-

F

-

E

R

D

E

I

I

Y

V

D

L

Y

V

C

L

E

E

K

E

M

M

E

G

L

F

N

E

V

V

D

E

T

A

L

A

I

H

H

H

E
|
E

V

V

G

A

A

F

G

G

Q

Q

M

H

E
|
E

I

I

N

D

F

F

F

K

H

Y

A

D

H

N

P

A

L

L

G

A

L

T

A

A

D

D

E

N

V

V

F

I

F

T

F

L

K

K

R

Y

T

V

V

A

R

K

E

T

A

L

A

A

L

L

R

Q

H

H

N

G

M

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

I

A

F

G

G

E

V

P

N

G

G

S

S

A

G

M

M

H

H

V

T

H

N

Q

T

S

S

I

L

L

F

S

-

K

-

E

K

T

D

G

G

L

K

N

N

I

A

F

F

S

Y

N

D

P

P

D

D

G

A

S

P

E

D

-

Q

-

I

S

S

E

D

A

T

F

L

F

R

H

Y

Y

F

I

V

G

G

L

V

Q

L

R

K

Y

H

I

I

P

R

A

A

A

I

M

T

A

A

L

I

V

T

A

N

P

P

Y

L

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

S

V

R

P

D

G

T

Y

A

E

A

A

P

P

I

V

N

Y

I

I

A

T

W

W

G

S

H

G

D

P

N

N

R

R

T

S

V

S

G

L

I

I

R

R

S

V

P

P

I

A

A

P

T

R

P

G

Q

N

A

S

R

T

R

R

V

I

E

E

N

I

R

R

V

S

I

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

V

L

A

A

L

F

A

A

M

A

T

I

L

L

A

A

C

A

G

G

Y

L

L

D

G

G

I

V

K

K

N

N

K

K

I

I

K

E

P

P

K

P

P

E

E

R

M

V

K

E

G

K

D

N

A

I

Y

Y

-

K

-

L

-

T

-

E

-

R

-

E

-

K

L

L

S

G

P

I

Y

G

S

M

L

L

P

P

R

G

S

T

L

L

G

K

E

E

A

A

L

I

D

E

W

C

L

F

R

K

R

E

E

D

S

E

D

L

L

L

H

V

E

S

V

A

L

L

G

G

R

E

E

H

F

V

V

S

T

Q

V

S

Y

I

S

I

E

N

I

V

K

A

E

M

M

A

E

D

F

W

A

D

E

S

F

Y

M

R

K

T

V

Q

I

V

S

H

P

Q

W

W

E

E

R

L

E

D

H

R

L

Y

L

L

binding magnesium ion: E129 (= E150), E183 (= E213), E190 (= E220)

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
30% identity, 97% coverage: 12:456/459 of query aligns to 4:440/443 of 4lnkA

query
sites
4lnkA

T

T

F

R

T

E

D

D

L

I

E

E

N

K

W

L

L

V

N

K

E

E

R

E

R

N

V

V

T

K

E

Y

V

I

E

R

C

L

L

Q

V

F

P

T

D

D

L

I

T

L

G

G

V

T

A

I

R

K

G

N

K

V

I

E

L

I

P

P

R

V

G

S

K

Q

F

L

T

-

E

-

D

-

R

-

G

G

M
x
K

R

A

L

L

P

D

E

N

A

K

V

V

V
x
M

A

F

M
x
D

G

G

V

S

T

S

G

I

E

E

F

-

P

-

E

-

S

-

G

-

P

-

Y

-

D

G

V

F

I

V

S

R

P

I

T

E

D

E

K

S

D

D

M

M

H

Y

L

L

R

Y

P

P

D

D

P

L

S

N

T

T

V

F

R

V

I

I

V

F

P

P

W

W

A

T

S

A

-

E

-

K

N

G

P

K

T

V

A

A

Q

R

V

F

I

I

H

C

D

D

C

I

F

Y

D

N

P

P

A

D

G

G

K

T

L

P

I

F

P

E

F

G

A

D

P

P

R

R

S

N

V

N

L

L

R

K

R

R

V

I

C

L

E

K

L

E

Y

M

E

E

A

D

E

L

G

G

L

F

V

S

P

D

V

F

-

N

V

L

A

G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

-

F

-

K

V

L

A

D

R

E

N

K

T

G

D

E

P

P

N

T

T

L

L

E

L

L

R

N

P

D

P

K

I

G

G

G

R

Y

S

F

G

D

R

L

A

A

E

P

T

T

S

D

R

L

Q

G

A

-

Y

-

S

-

I

-

D

-

A

-

V

-

N

-

E

-

F

-

D

E

P

N

L

C

F

R

E

R

D

D

I

I

Y

V

D

L

Y

E

C

L

E

E

K

E

M

M

E

G

L

F

N

E

V

I

D

E

T

A

L

S

I

H

H

H

E
|
E

V
|
V

G

A

A

P

G

G

Q

Q

M

H

E
|
E

I

I

N

D

F
|
F

F

K

H
x
Y

A

A

H

G

P

A

L

V

G

R

L

S

A

C

D

D

E

D

V

I

F

Q

F

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

A

I

A

A

L

R

R

K

H

H

N

G

M

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

G

G

E

V

P
x
N

G
|
G

S

S

A
x
G

M

M

H
|
H

V

C

H
x
N

Q

L

S
|
S

I

L

L

F

S

-

K

-

E

K

T

N

G

G

L

V

N

N

I

A

F

F

-

F

-

D

S

E

N

N

P

A

D

D

G

L

S

Q

E

L

S

S

E

E

A

T

F

A

F

K

H

H

Y

F

I

I

G

A

G

G

L

I

Q

V

R

K

Y

H

I

A

P

T

A

S

A

F

M

T

A

A

L

V

V

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

S

V

R

P

D

G

T

Y

A
x
E

A

A

P

P

I

C

N

Y

I

V

A

A

W

W

G

S

H

A

D

Q

N

N

R
|
R

T

S

V

P

G

L

I

I

R

R

S

I

P

P

I

A

A
x
S

T

R

P

G

Q

I

A
x
S

R

T

R
|
R

V

V

E
|
E

N

V

R
|
R

V

S

I

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

V

L

A

A

L

L

A

S

M

V

T

L

L

L

A

A

C

A

G

G

Y

L

L

D

G

G

I

I

K

K

N

N

K

K

I

L

K

E

P

A

K

P

P

A

E

P

M

I

K

D

G

R

D

N

A

I

Y

Y

L

V

S

M

P

S

-

K

-

E

-

R

-

M

-

E

-

N

-

G

-

I

Y

V

S

D

L

L

P

P

R

A

S

T

L

L

G

A

E

E

A

A

L

L

D

E

W

E

L

F

R

K

R

S

E

N

S

E

D

V

L

M

H

V

E

K

V

A

L

L

G

G

R

E

E

H

F

L

V

F

T

E

V

H

Y

F

S

I

E

E

I

A

K

K

E

E

M

I

E

E

F

W

A

D

E

M

F

F

M

R

K

T

V

Q

I

V

S

H

P

P

W

W

E

E

R

R

E

E

H

Q

L

Y

L

M

active site: D52 (≠ M64), E131 (= E148), E133 (= E150), E188 (= E213), E195 (= E220), H244 (= H269), R315 (= R340), E332 (= E357), R334 (= R359)
binding adenosine-5'-diphosphate: K43 (≠ M55), M50 (≠ V62), F198 (= F223), Y200 (≠ H225), N246 (≠ H271), S248 (= S273), S324 (≠ A349), S328 (≠ A353), R330 (= R355)
binding glutamic acid: E133 (= E150), E188 (= E213), V189 (= V214), N239 (≠ P264), G240 (= G265), G242 (≠ A267), E303 (≠ A328)
binding magnesium ion: E131 (= E148), E188 (= E213), E195 (= E220), H244 (= H269), E332 (= E357)

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
30% identity, 97% coverage: 12:456/459 of query aligns to 4:440/443 of 4lniA

query
sites
4lniA

T

T

F

R

T

E

D

D

L

I

E

E

N

K

W

L

L

V

N

K

E

E

R

E

R

N

V

V

T

K

E

Y

V

I

E

R

C

L

L

Q

V

F

P

T

D

D

L

I

T

L

G

G

V

T

A

I

R

K

G

N

K

V

I

E

L

I

P

P

R

V

G

S

K

Q

F

L

T

-

E

-

D

-

R

-

G

G

M

K

R

A

L

L

P

D

E

N

A

K

V

V

V

M

A

F

M
x
D

G

G

V

S

T

S

G

I

E

E

F

-

P

-

E

-

S

-

G

-

P

-

Y

-

D

G

V

F

I

V

S

R

P

I

T

E

D

E

K

S

D

D

M

M

H

Y

L

L

R

Y

P

P

D

D

P

L

S

N

T

T

V

F

R

V

I

I

V

F

P

P

W

W

A

T

S

A

-

E

-

K

N

G

P

K

T

V

A

A

Q

R

V

F

I

I

H

C

D

D

C

I

F

Y

D

N

P

P

A

D

G

G

K

T

L

P

I

F

P

E

F

G

A

D

P

P

R

R

S

N

V

N

L

L

R

K

R

R

V

I

C

L

E

K

L

E

Y

M

E

E

A

D

E

L

G

G

L

F

V

S

P

D

V

F

-

N

V

L

A

G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

-

F

-

K

V

L

A

D

R

E

N

K

T

G

D

E

P

P

N

T

T

L

L

E

L

L

R

N

P

D

P

K

I

G

G

G

R

Y

S

F

G

D

R

L

A

A

E

P

T

T

S

D

R

L

Q

G

A

-

Y

-

S

-

I

-

D

-

A

-

V

-

N

-

E

-

F

-

D

E

P

N

L

C

F

R

E

R

D

D

I

I

Y

V

D

L

Y

E

C

L

E

E

K

E

M

M

E

G

L

F

N

E

V

I

D
x
E

T

A

L

S

I

H

H

H

E
|
E

V

V

G

A

A

P

G

G

Q

Q

M

H

E
|
E

I

I

N
x
D

F

F

F

K

H
x
Y

A

A

H

G

P

A

L

V

G

R

L

S

A

C

D

D

E

D

V

I

F

Q

F

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

A

I

A

A

L

R

R

K

H

H

N

G

M

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

G

G

E

V

P

N

G

G

S

S

A

G

M

M

H
|
H

V

C

H
x
N

Q

L

S
|
S

I

L

L

F

S

-

K

-

E

K

T

N

G

G

L

V

N

N

I

A

F

F

-

F

-

D

S

E

N

N

P

A

D

D

G

L

S

Q

E

L

S

S

E

E

A

T

F

A

F

K

H

H

Y

F

I

I

G

A

G

G

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I

Q

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R

K

Y

H

I

A

P

T

A

S

A

F

M

T

A

A

L

V

V

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

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R

L

L

S

V

R

P

D

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T

Y

A
x
E

A

A

P

P

I

C

N

Y

I

V

A

A

W

W

G

S

H

A

D

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N

N

R
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R

T

S

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P

G

L

I

I

R
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R

S

I

P

P

I

A

A

S

T

R

P

G

Q

I

A

S

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T

R
|
R

V

V

E
|
E

N

V

R
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R

V

S

I

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

V

L

A

A

L

L

A

S

M

V

T

L

L

L

A

A

C

A

G

G

Y

L

L

D

G

G

I

I

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K

N

N

K

K

I

L

K

E

P

A

K

P

P

A

E

P

M

I

K

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G

R

D

N

A

I

Y

Y

L

V

S

M

P

S

-

K

-

E

-

R

-

M

-

E

-

N

-

G

-

I

Y

V

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D

L

L

P

P

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A

S

T

L

L

G

A

E

E

A

A

L

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W

E

L

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R

K

R

S

E

N

S

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M

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V

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K

V

A

L

L

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G

R

E

E

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L

V

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T

E

V

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Y

F

S

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E

I

A

K

K

E

E

M

I

E

E

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W

A

D

E

M

F

F

M

R

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W

W

E

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R

R

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active site: D52 (≠ M64), E131 (= E148), E133 (= E150), E188 (= E213), E195 (= E220), H244 (= H269), R315 (= R340), E332 (= E357), R334 (= R359)
binding adenosine-5'-diphosphate: E131 (= E148), E183 (≠ D208), D197 (≠ N222), Y200 (≠ H225), N246 (≠ H271), S248 (= S273), R320 (= R345), R330 (= R355)
binding magnesium ion: E131 (= E148), E131 (= E148), E133 (= E150), E188 (= E213), E195 (= E220), E195 (= E220), H244 (= H269), E332 (= E357)
binding l-methionine-s-sulfoximine phosphate: E133 (= E150), E188 (= E213), H244 (= H269), R297 (= R322), E303 (≠ A328), R315 (= R340), R334 (= R359)

P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 5 papers)
30% identity, 97% coverage: 12:456/459 of query aligns to 5:441/444 of P12425

query
sites
P12425

T

T

F

R

T

E

D

D

L

I

E

E

N

K

W

L

L

V

N

K

E

E

R

E

R

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E

Y

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E

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L

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D

L

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L

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K

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R

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T

-

E

-

D

-

R

-

G

G

M

K

R

A

L

L

P

D

E

N

A

K

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V

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M

A

F

M

D

G

G

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T

S

G

I

E

E

F

-

P

-

E

-

S

-

G

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P

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Y

-

D
x
G

V

F

I

V

S
x
R

P

I

T

E

D

E

K

S

D

D

M

M

H

Y

L

L

R

Y

P

P

D

D

P

L

S

N

T

T

V

F

R

V

I

I

V

F

P

P

W

W

A

T

S

A

-

E

-

K

N

G

P

K

T

V

A

A

Q

R

V

F

I

I

H

C

D

D

C

I

F

Y

D

N

P

P

A

D

G

G

K

T

L

P

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E

F

G

A

D

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P

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N

L

L

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R

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V

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C

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K

L

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Y

M

E

E

A

D

E

L

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G

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D

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-

N

V

L

A

G

P

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|
E

L

P

E
|
E

F

F

Y

F

L

L

-

F

-

K

V

L

A

D

R

E

N

K

T

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P

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T

T

L

L

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P

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I

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G

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G

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R

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A

A

E

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T

S

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R

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G

A

-

Y

-

S

-

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-

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-

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-

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D

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Y

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A

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E

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V

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A

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E

I

I

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D

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A

A

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A

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A

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F

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M

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K

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F

G

G

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G
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G

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G

M

M

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H

V

C

H

N

Q

L

S

S

I

L

L

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S

-

K

-

E

K

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N

G

G

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P

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E

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L

L

A

A

C

A

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G

G

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I

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K

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N

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A

K

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A

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M

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K

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A

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Y

L

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P

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K

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M

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E

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G

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A

A

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E

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F

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G59 (≠ D78) mutation to R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
R62 (≠ S81) Important for inhibition by glutamine; mutation to A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.
E132 (= E148) binding Mg(2+)
E134 (= E150) binding Mg(2+)
E189 (= E213) binding Mg(2+)
V190 (= V214) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E220) binding Mg(2+)
G241 (= G265) binding L-glutamate
H245 (= H269) binding Mg(2+)
G302 (≠ D326) mutation to E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
E304 (≠ A328) mutation to A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
P306 (= P330) mutation to H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
E333 (= E357) binding Mg(2+)
E424 (= E439) mutation to K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4s0rD Structure of gs-tnra complex (see paper)
30% identity, 97% coverage: 12:456/459 of query aligns to 8:444/447 of 4s0rD

query
sites
4s0rD

T

T

F

R

T

E

D

D

L

I

E

E

N

K

W

L

L

V

N

K

E

E

R

E

R

N

V

V

T

K

E

Y

V

I

E

R

C

L

L

Q

V

F

P

T

D

D

L

I

T

L

G

G

V

T

A

I

R

K

G

N

K

V

I

E

L

I

P

P

R

V

G

S

K

Q

F

L

T

-

E

-

D

-

R

-

G

G

M

K

R

A

L

L

P

D

E

N

A

K

V

V

V

M

A

F

M
x
D

G

G

V

S

T

S

G

I

E

E

F

-

P

-

E

-

S

-

G

-

P

-

Y

-

D

G

V
x
F

I
x
V

S
x
R

P
x
I

T

E

D

E

K

S

D

D

M

M

H

Y

L

L

R

Y

P

P

D

D

P

L

S

N

T

T

V

F

R

V

I

I

V

F

P

P

W

W

A

T

S

A

-

E

-

K

N

G

P

K

T

V

A

A

Q

R

V

F

I

I

H

C

D

D

C

I

F

Y

D

N

P

P

A

D

G

G

K

T

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P

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F

G

A

D

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P

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N

V

N

L

L

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K

L

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M

E

E

A

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G

L

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D

V

F

-

N

V

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A

G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

-

F

-

K

V

L

A

D

R

E

N

K

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G

D

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P

P

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T

L

L

E

L

L

R

N

P

D

P

K

I

G

G

G

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G
x
D

R

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A

A

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R

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G

A

-

Y

-

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-

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E

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E

I

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Y

A

A

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A

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G

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H

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L

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N

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H

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A

A

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P

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V

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N

S

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Y

R

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R
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L

L

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D
x
G

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x
Y

A
x
E

A

A

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P

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A

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E

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R

V

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D

A

P

D

A

A

A

N

N

P

P

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Y

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A

A

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L

L

L

A

A

C

A

G

G

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L

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D

G

G

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I

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K

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N

K

K

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A

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K

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D

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Y

L

V

S

M

P

S

-

K

-

E

-

R

-

M

-

E

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-

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-

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A

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E

A

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E

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S

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x
H

Y

F

S

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E

E

I

A

K

K

E

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M
x
I

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x
M

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active site: D56 (≠ M64), E135 (= E148), E137 (= E150), E192 (= E213), E199 (= E220), H248 (= H269), R319 (= R340), E336 (= E357), R338 (= R359)
binding glutamine: E137 (= E150), E192 (= E213), R301 (= R322), E307 (≠ A328)
binding magnesium ion: I66 (≠ P82), E135 (= E148), E135 (= E148), E199 (= E220), H248 (= H269), H248 (= H269), E336 (= E357), H419 (≠ V431)
binding : F63 (≠ V79), V64 (≠ I80), R65 (≠ S81), I66 (≠ P82), D161 (≠ G172), G241 (= G262), V242 (≠ E263), N243 (≠ P264), G305 (≠ D326), Y306 (≠ T327), Y376 (= Y397), I426 (≠ M438), M430 (≠ E442)

7tfaB Glutamine synthetase (see paper)
33% identity, 82% coverage: 84:458/459 of query aligns to 63:440/441 of 7tfaB

query
sites
7tfaB

D

E

K

S

D

D

M

M

H

Y

L

L

R

Y

P

P

D

D

P

L

S

D

T

T

V

W

R

V

I

V

V

F

P

P

W

W

-

V

A

T

S

S

N

D

P

R

T

V

A

A

Q

R

V

L

I

I

H

C

D

D

C

I

F

Y

D

K

P

P

A

D

G

G

K

S

L

-

I

-

P

P

F

F

A

A

-

G

-

D

P

P

R

R

S

G

V

I

L

L

R

K

R

R

V

V

C

L

E

K

L

E

Y

A

E

E

A

E

E

L

G

G

L

Y

V

T

P

S

V

M

-

N

V

V

A

G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

V

F

A

K

R

T

N

D

T

E

-

K

-

G

D

D

P

P

N

T

T

T

L

E

L

L

R

N

P

D

P

Q

I

G

G

G

R

-

S

-

G

-

R

-

A

-

E

-

T

-

S

-

R

-

Q

-

A
x
Y

Y

F

S

D

I

L

D

A

A

P

V

M

N

D

E

L

F

G

D

E

P

N

L

C

F

R

E

R

D

E

I

I

Y

V

D

L

Y

K

C

L

E

E

K

E

M

M

E

G

L

F

N

E

V

I

D

E

T

A

L

S

I

H

H

H

E
|
E

V
|
V

G

A

A

P

G

G

Q

Q

M

H

E
|
E

I

I

N

D

F

F

F

K

H

Y

A

A

H

D

P

A

L

V

G

K

L

A

A

A

D

D

E

Q

V

I

F

Q

F

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

A

I

A

A

L

R

R

Q

H

H

N

G

M

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

G

G

E

V

P
x
N

G
|
G

S

S

A

G

M

M

H
|
H

V

C

H

N

Q

Q

S

S

I

L

L

F

S

K

K

D

E

N

T

E

G

N

L

V

N

F

I

Y

F

D

S

E

N

T

P

D

D

E

G

L

S

G

E

L

S

S

E

Q

A

T

F

A

F

R

H

H

Y

Y

I

M

G

A

G

G

L

I

Q

L

R

K

Y

H

I

A

P

R

A

A

A

M

M

A

A

A

L

I

V

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

S

V

R

P

D
x
G

T
x
Y

A
x
E

A

A

P

P

I

C

N

Y

I

V

A

A

W

W

G

S

H

A

D

S

N

N

R
|
R

T

S

V

P

G

M

I

I

R

R

S

I

P

P

I

A

A

S

T

R

P

G

Q

L

A

S

R

T

R

R

V

V

E
|
E

N

V

R

R

V

N

I

P

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

V

L

A

A

L

L

A

A

M

V

T

M

L

L

A

R

C

A

G

G

Y

L

L

D

G

G

I

I

K

K

N

R

K

Q

I

M

K

A

-

L

P

P

K

A

P

P

E

I

M

D

K

R

G

N

D

I

A

Y

Y

V

L

M

S

S

P

E

Y

E

-

E

-

R

-

I

-

E

-

G

-

I

-

P

S

S

L

L

P

P

R

A

S

D

L

L

G

K

E

E

A

A

L

L

D

S

W

E

L

L

R

I

R

R

E

S

S

E

D

V

L

I

H

S

E

D

V

A

L

L

G

G

R

D

E

H

F

A

V

L

T

A

V

Y

Y

F

S

Y

E

E

I

L

K

K

E

E

M

I

E

E

F

W

A

D

E
x
M

F

Y

M

R

K

T

V

Q

I

V

S

H

P

Q

W

W

E

E

R

R

E

D

H

Q

L

Y

L

L

L

T

L

L

binding glutamine: E131 (= E150), Y153 (≠ A180), E186 (= E213), G238 (= G265), H242 (= H269), R295 (= R322), E301 (≠ A328)
binding magnesium ion: E129 (= E148), E131 (= E150), E186 (= E213), E193 (= E220), H242 (= H269), E330 (= E357)
binding : V187 (= V214), N237 (≠ P264), G299 (≠ D326), Y300 (≠ T327), R313 (= R340), M424 (≠ E442)

Sites not aligning to the query:

binding : 58, 60

7tdpA Structure of paenibacillus polymyxa gs bound to met-sox-p-adp (transition state complex) to 1.98 angstom (see paper)
33% identity, 82% coverage: 84:458/459 of query aligns to 63:438/439 of 7tdpA

query
sites
7tdpA

D

E

K

S

D

D

M

M

H

Y

L

L

R

Y

P

P

D

D

P

L

S

D

T

T

V

W

R

V

I

V

V

F

P

P

W

W

A

-

S

S

N

D

P

R

T

V

A

A

Q

R

V

L

I

I

H

C

D

D

C

I

F

Y

D

K

P

P

A

D

G

G

K

S

L

-

I

-

P

P

F

F

A

A

-

G

-

D

P

P

R

R

S

G

V

I

L

L

R

K

R

R

V

V

C

L

E

K

L

E

Y

A

E

E

A

E

E

L

G

G

L

Y

V

T

P

S

V

M

-
x
N

V

V

A
x
G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

V

F

A

K

R

T

N

D

T

E

-

K

-

G

D

D

P

P

N

T

T

T

L

E

L

L

R

N

P

D

P

Q

I

G

G

G

R

-

S

-

G

-

R

-

A

-

E

-

T

-

S

-

R

-

Q

-

A

Y

Y

F

S

D

I

L

D

A

A

P

V

M

N

D

E

L

F

G

D

E

P

N

L

C

F

R

E

R

D

E

I

I

Y

V

D

L

Y

K

C

L

E

E

K

E

M

M

E

G

L

F

N

E

V

I

D
x
E

T

A

L

S

I

H

H

H

E
|
E

V

V

G

A

A

P

G

G

Q

Q

M

H

E
|
E

I

I

N
x
D

F

F

F

K

H
x
Y

A

A

H

D

P

A

L

V

G

K

L

A

A

A

D

D

E

Q

V

I

F

Q

F

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

A

I

A

A

L

R

R

Q

H

H

N

G

M

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

G

G

E

V

P

N

G
|
G

S

S

A

G

M

M

H
|
H

V

C

H
x
N

Q

Q

S
|
S

I

L

L

F

S

K

K

D

E

N

T

E

G

N

L

V

N

F

I

Y

F

D

S

E

N

T

P

D

D

E

G

L

S

G

E

L

S

S

E

Q

A

T

F

A

F

R

H

H

Y

Y

I

M

G

A

G

G

L

I

Q

L

R

K

Y

H

I

A

P

R

A

A

A

M

M

A

A

A

L

I

V

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

S

V

R

P

D

G

T

Y

A
x
E

A

A

P

P

I

C

N

Y

I

V

A

A

W

W

G

S

H

A

D

S

N

N

R
|
R

T

S

V

P

G

M

I

I

R
|
R

S

I

P

P

I

A

A

S

T

R

P

G

Q

L

A

S

R

T

R
|
R

V

V

E
|
E

N

V

R
|
R

V

N

I

P

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

V

L

A

A

L

L

A

A

M

V

T

M

L

L

A

R

C

A

G

G

Y

L

L

D

G

G

I

I

K

K

N

R

K

Q

I

M

K

A

-

L

P

P

K

A

P

P

E

I

M

D

K

R

G

N

D

I

A

Y

Y

V

L

M

S

S

P

E

Y

E

-

E

-

R

-

I

-

E

-

G

-

I

-

P

S

S

L

L

P

P

R

A

S

D

L

L

G

K

E

E

A

A

L

L

D

S

W

E

L

L

R

I

R

R

E

S

S

E

D

V

L

I

H

S

E

D

V

A

L

L

G

G

R

D

E

H

F

A

V

L

T

A

V

Y

Y

F

S

Y

E

E

I

L

K

K

E

E

M

I

E

E

F

W

A

D

E

M

F

Y

M

R

K

T

V

Q

I

V

S

H

P

Q

W

W

E

E

R

R

E

D

H

Q

L

Y

L

L

L

T

L

L

binding adenosine-5'-diphosphate: N123 (vs. gap), G125 (≠ A146), E127 (= E148), E179 (≠ D208), D193 (≠ N222), Y196 (≠ H225), N242 (≠ H271), S244 (= S273), R316 (= R345), R326 (= R355)
binding magnesium ion: E127 (= E148), E127 (= E148), E129 (= E150), E184 (= E213), E191 (= E220), E191 (= E220), H240 (= H269), E328 (= E357)
binding l-methionine-s-sulfoximine phosphate: E127 (= E148), E129 (= E150), E184 (= E213), E191 (= E220), G236 (= G265), H240 (= H269), R293 (= R322), E299 (≠ A328), R311 (= R340), R330 (= R359)

5dm3C Crystal structure of glutamine synthetase from chromohalobacter salexigens dsm 3043(csal_0679, target efi-550015) with bound adp
32% identity, 79% coverage: 89:451/459 of query aligns to 56:390/396 of 5dm3C

query
sites
5dm3C

L

M

R

K

P

P

D

D

P

L

S

A

T

T

V

L

R

R

I

C

V

V

P

P

W

W

A

L

S

E

N

G

P

-

T

T

A

A

Q

M

V

V

I

L

H

C

D

D

C

L

F

L

D

D

P

H

A

R

G

T

K

H

L

A

-

E

I

V

P

P

F

H

A

A

P

P

R

R

S

A

V

I

L

L

R

K

R

R

V

Q

C

L

E

A

L

R

Y

L

E

E

A

A

E

M

G

G

L

L

V

E

P

A

V

I

V

M

A

A

P

T

E
|
E

L

L

E
|
E

F

F

Y

F

L

L

V

F

A

E

R

K

N

S

T

L

D

D

P

E

N

I

T

-

L

-

R

-

P

-

I

-

G

-

R

R

S

K

G

G

R

R

A

F

E

R

T

T

S

T

R

K

Q

E

A

E

Y

H

S

-

I

-

D

-

A

-

V

-

N

-

E

-

F

-

D

-

P

-

L

V

F

L

E

R

D

P

I

L

Y

R

D

N

Y

H

C

L

E

H

K

A

M

A

E

G

L

I

N

P

V

V

D

E

T

G

L

T

I

K

H

G

E
|
E

V

A

G

G

A

A

G

G

Q

Q

M

E

E
|
E

I

L

N

N

F

I

F
x
R

H
x
C

A

A

H

K

P

A

L

L

G

D

L

T

A

A

D

D

E

Y

V

H

F

T

F

I

F

A

K

K

R

H

T

A

V

T

R

K

E

E

A

I

A

A

L

W

R

Q

H

Q

N

G

M

R

Y

A

A

V

T

T

F

F

M

L

A

S

K

K

P

W

I

H

A

H

G

A

E

H

P

A

G

G

S

S

A

S

M

S

H
|
H

V

I

H
|
H

Q

Q

S
|
S

I

L

L

W

S

K

K

Q

E

-

T

-

G

G

L

L

N

P

I

A

F

F

S

H

N

D

P

E

D

R

G

D

S

A

-

L

-

G

E

M

S

S

E

A

A

L

F

M

F

K

H

H

Y

Y

I

L

G

A

G

G

L

L

Q

L

R

K

Y

Y

I

A

P

P

A

D

A

Y

M

T

A

Y

L

F

V

L

A

A

P

P

Y

Y

V

L

N

N

S

S

Y

Y

R

K

R

R

L

F

S

Q

R

-

D

-

T

-

A

F

A

A

P

P

I

T

N

R

I

T

A

V

W

W

G

S

H

V

D

D

N

N

R
|
R

T

T

V

A

G

G

I

F

R

R

S

L

P

C

I

A

A

E

T

G

P

T

Q

R

A

A

R

V

R
|
R

V

I

E
|
E

N

C

R
|
R

V

I

I

G

G

G

A

S

D

D

A

L

N

N

P

P

Y

Y

V

L

A

A

L

M

A

A

M

G

T

Q

L

L

A

A

C

A

G

G

Y

I

L

K

G

G

I

I

K

E

N

E

K

C

I

L

K

A

P

L

K

P

P

P

E

P

M

A

K

S

G

G

D

-

A

-

Y

-

L

-

S

-

P

-

Y

-

S

L

L

I

P

P

R

Q

S

N

L

L

G

R

E

D

A

A

L

M

D

E

W

A

L

L

R

R

R

G

E

S

S

T

D

M

L

L

H

R

E

E

V

A

L

M

G

G

R

E

E

D

F

V

V

V

T

D

V

H

Y

Y

S

V

E

R

I

A

K

A

E

E

M

V

E

E

F

L

A

E

E

D

F

F

M

Q

K

R

V

V

I

V

S

S

P

D

W

Y

E

E

active site: E115 (= E148), E117 (= E150), E162 (= E213), E169 (= E220), H218 (= H269), R286 (= R340), E303 (= E357), R305 (= R359)
binding adenosine-5'-diphosphate: R173 (≠ F224), C174 (≠ H225), H220 (= H271), S222 (= S273), R301 (= R355)

7tenA Glutamine synthetase (see paper)
31% identity, 81% coverage: 84:456/459 of query aligns to 63:439/442 of 7tenA

query
sites
7tenA

D

E

K

S

D

D

M

M

H

Y

L

L

R

F

P

P

D

D

P

L

S

D

T

T

V

W

R

V

I

V

V

F

P

P

W

W

A

T

S

A

-

E

-

K

N

G

P

K

T

V

A

A

Q

R

V

M

I

I

H

C

D

D

C

I

F

Y

D

N

P

P

A

-

G

-

K

D

L

M

I

T

P

P

F

F

A

A

-

G

-

D

P

P

R

R

S

A

V

N

L

L

R

K

R

R

V

V

C

L

E

K

L

E

Y

M

E

E

A

E

E

L

G

G

L

F

V

T

P

E

V

F

-

N

V

L

A
x
G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

V

F

A

-

R

-

N

K

T

L

D

D

P

E

N

N

T

R

L

-

R

R

P

P

P

T

I

L

G

-

R

-

S

-

G

-

R

E

A

L

E

N

T

D

S

S

R

G

Q

G

A

Y

Y

F

S

D

I

L

D

A

A

P

V

T

N

D

E

L

F

G

D

E

P

N

L

C

F

R

E

R

D

D

I

I

Y

V

D

L

Y

E

C

L

E

E

K

E

M

M

E

G

L

F

N

E

V

I

D
x
E

T

A

L

S

I

H

H

H

E
|
E

V

V

G

A

A

P

G

G

Q

Q

M

H

E
|
E

I

I

N
x
D

F
|
F

F
x
K

H
x
Y

A

E

H

D

P

A

L

I

G

T

L

A

A

C

D

D

E

S

V

I

F

Q

F

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

A

I

A

A

L

R

R

K

H

H

N

G

M

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

G

G

E

V

P
x
N

G
|
G

S

S

A

G

M

M

H
|
H

V

F

H
x
N

Q

M

S
|
S

I

L

L

F

S

N

K

-

E

E

T

K

G

G

L

N

N

A

I

F

F

F

S

D

N

E

P

S

D

G

G

E

S

L

E

E

-

L

S

S

E

Q

A

T

F

A

F

Y

H

H

Y

F

I

L

G

A

G

G

L

M

Q

L

R

K

Y

H

I

A

P

R

A

G

A

Y

M

T

A

A

L

V

V

T

A

N

P

P

Y

T

V

I

N

N

S

S

Y

F

R

K

R
|
R

L

L

S

V

R

P

D

G

T

Y

A
x
E

A

A

P

P

I

C

N

Y

I

I

A

A

W

W

G

S

H

G

D

K

N

N

R
|
R

T

S

V

P

G

L

I

V

R
|
R

S

V

P

P

I

S

A

S

T

R

P

G

Q

L

A
x
S

R

T

R
|
R

V

L

E

E

N

L

R
|
R

V

S

I

V

G

D

A

P

D

S

A

A

N

N

P

P

Y

Y

V

L

A

A

L

M

A

A

M

V

T

L

L

L

A

K

C

A

G

G

Y

L

L

S

G

G

I

I

K

K

N

D

K

E

I

L

K

T

P

P

K

P

P

A

E

P

M

V

K

D

G

R

D

N

A

I

Y

Y

-

G

-

M

-

N

-

E

-

R

-

E

-

A

L

T

S

G

P

I

Y

Y

S

D

L

L

P

P

R

E

S

S

L

L

G

G

E

H

A

A

L

L

D

I

W

E

L

L

R

E

R

K

E

N

S

E

D

I

L

I

H

K

E

D

V

G

L

L

G

G

R

E

E

H

F

I

V

F

T

E

V

H

Y

F

S

I

E

E

I

A

K

K

E

T

M

I

E

E

F

C

A

D

E

M

F

F

M

R

K

T

V

A

I

V

S

H

P

P

W

W

E

E

R

R

E

E

H

Q

L

Y

L

L

binding adenosine-5'-diphosphate: G128 (≠ A146), E130 (= E148), E182 (≠ D208), D196 (≠ N222), F197 (= F223), K198 (≠ F224), Y199 (≠ H225), N245 (≠ H271), S247 (= S273), R319 (= R345), S327 (≠ A353), R329 (= R355)
binding l-methionine-s-sulfoximine phosphate: E130 (= E148), E132 (= E150), E187 (= E213), E194 (= E220), N238 (≠ P264), G239 (= G265), H243 (= H269), R296 (= R322), E302 (≠ A328), R314 (= R340), R333 (= R359)

7tf9S L. Monocytogenes gs(14)-q-glnr peptide (see paper)
31% identity, 81% coverage: 84:456/459 of query aligns to 64:440/443 of 7tf9S

query
sites
7tf9S

D

E

K

S

D

D

M

M

H

Y

L

L

R

F

P

P

D

D

P

L

S

D

T

T

V

W

R

V

I

V

V

F

P

P

W

W

A

T

S

A

-

E

-

K

N

G

P

K

T

V

A

A

Q

R

V

M

I

I

H

C

D

D

C

I

F

Y

D

N

P

P

A

-

G

-

K

D

L

M

I

T

P

P

F

F

A

A

-

G

-

D

P

P

R

R

S

A

V

N

L

L

R

K

R

R

V

V

C

L

E

K

L

E

Y

M

E

E

A

E

E

L

G

G

L

F

V

T

P

E

V

F

-

N

V

L

A

G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

V

F

A

-

R

-

N

K

T

L

D

D

P

E

N

N

T

R

L

-

R

R

P

P

P

T

I

L

G

-

R

-

S

-

G

-

R

E

A

L

E

N

T

D

S

S

R

G

Q

G

A
x
Y

Y

F

S

D

I

L

D

A

A

P

V

T

N

D

E

L

F

G

D

E

P

N

L

C

F

R

E

R

D

D

I

I

Y

V

D

L

Y

E

C

L

E

E

K

E

M

M

E

G

L

F

N

E

V

I

D

E

T

A

L

S

I

H

H

H

E
|
E

V

V

G

A

A

P

G

G

Q

Q

M

H

E
|
E

I

I

N

D

F

F

F

K

H

Y

A

E

H

D

P

A

L

I

G

T

L

A

A

C

D

D

E

S

V

I

F

Q

F

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

A

I

A

A

L

R

R

K

H

H

N

G

M

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

G

G

E

V

P

N

G
|
G

S

S

A
x
G

M

M

H
|
H

V

F

H

N

Q

M

S

S

I

L

L

F

S

N

K

-

E

E

T

K

G

G

L

N

N

A

I

F

F

F

S

D

N

E

P

S

D

G

G

E

S

L

E

E

-

L

S

S

E

Q

A

T

F

A

F

Y

H

H

Y

F

I

L

G

A

G

G

L

M

Q

L

R

K

Y

H

I

A

P

R

A

G

A

Y

M

T

A

A

L

V

V

T

A

N

P

P

Y

T

V

I

N

N

S

S

Y

F

R

K

R
|
R

L

L

S

V

R

P

D

G

T

Y

A
x
E

A

A

P

P

I

C

N

Y

I

I

A

A

W

W

G

S

H

G

D

K

N

N

R

R

T

S

V

P

G

L

I

V

R

R

S

V

P

P

I

S

A

S

T

R

P

G

Q

L

A

S

R

T

R

R

V

L

E
|
E

N

L

R

R

V

S

I

V

G

D

A

P

D

S

A

A

N

N

P

P

Y

Y

V

L

A

A

L

M

A

A

M

V

T

L

L

L

A

K

C

A

G

G

Y

L

L

S

G

G

I

I

K

K

N

D

K

E

I

L

K

T

P

P

K

P

P

A

E

P

M

V

K

D

G

R

D

N

A

I

Y

Y

-

G

-

M

-

N

-

E

-

R

-

E

-

A

L

T

S

G

P

I

Y

Y

S

D

L

L

P

P

R

E

S

S

L

L

G

G

E

H

A

A

L

L

D

I

W

E

L

L

R

E

R

K

E

N

S

E

D

I

L

I

H

K

E

D

V

G

L

L

G

G

R

E

E

H

F

I

V

F

T

E

V

H

Y

F

S

I

E
|
E

I

A

K

K

E

T

M
x
I

E

E

F

C

A

D

E
x
M

F

F

M

R

K

T

V

A

I

V

S

H

P

P

W

W

E

E

R

R

E

E

H

Q

L

Y

L

L

binding glutamine: E133 (= E150), Y155 (≠ A180), E188 (= E213), G240 (= G265), G242 (≠ A267), R297 (= R322), E303 (≠ A328)
binding magnesium ion: E131 (= E148), E133 (= E150), E188 (= E213), E195 (= E220), H244 (= H269), E332 (= E357)
binding : E418 (= E434), I422 (≠ M438), M426 (≠ E442)

Sites not aligning to the query:

binding : 59, 60

8wwuB Glutamine synthetase
31% identity, 93% coverage: 19:444/459 of query aligns to 11:466/492 of 8wwuB

query
sites
8wwuB

W

W

L

T

N

N

E

E

R

Q

R

R

-

D

-

A

-

D

-

K

-

V

V

L

T

A

E

E

V

I

E

D

C

S

L

L

-

G

-

L

-

E

-

M

-

I

-

R

-

L

-

S

V

W

P

A

D

D

L

Q

T

Y

G

G

V

L

A

L

R

R

G

G

K

K

I

S

L

L

P

T

-

V

-

A

-

S

-

L

R

K

G

S

K

A

F

F

T

K

E

E

D

G

R

S

G

E

M

V

R

A

L

I

P

G

E

P

-

F

A

D

V

L

V

V

A

S

M

S

G

M

V

V

T

F

G

N

E

L

F

F

P

T

E

T

S

A

G

G

P

D

Y

F

Y

E

D

D

V

E

I

L

S

S

-

G

-

N

P

P

T

T

D

-

K

-

D

-

M

V

H

V

L

M

R

V

P

P

D

D

P

P

S

T

T

T

V

F

R

K

I

V

V

L

P

P

W

W

A

A

S

-

N

D

P

K

T

T

A

G

Q

W

V

M

I

L

H

A

D

D

C

L

F

H

D

W

P

K

A

S

G

G

K

E

L

P

I

F

P

P

F

L

A

C

P

P

R

R

S

G

V

I

L

M

R

K

V

A

C

V

E

K

L

S

Y

L

E

S

A

D

E

E

G

G

L

Y

V

L

P

F

V

K

V

C

A
x
G

P

I

E
|
E

L

L

E
|
E

F

W

Y

Y

L

L

-

T

-

K

-

I

V

V

A

D

R

R

N

S

T

L

D

S

P

P

N

E

T

S

L

L

L

G

R

A

P

P

P

G

I

V

G

Q

R

P

S

D

G

A

-

I

R

Q

A

V

E

Q

T

P

S

V

R

A

Q

Q

A

G

Y

Y

S

S

I

V

D

L

A

L

V

E

N

H

E

H

F

L

D

D

P

Q

L

V

F

-

E

D

D

D

I

I

Y

M

D

S

Y

K

C

V

E

R

K

K

-

G

-

L

M

L

E

E

L

L

N

N

V

L

-

P

-

L

D
x
R

T

S

L

I

I

E

H

D

E
|
E

V

W

G

A

A

P

G

S

Q

Q

M

M

E
|
E

I

T

N

T

F
|
F

F

D

H
x
V

A

M

H

E

P

G

L

L

G

E

L

A

A

A

D

D

E

A

V

A

F

L

F

I

K

K

R

S

T

A

V

I

R

K

E

Q

A

I

A

C

L

S

R

R

H

H

N

G

M

Y

Y

H

A

A

T

T

F

F

M

M

A

C

K

K

P

P

-

A

I

I

A

N

G

G

E

F

P

F

G

A

S

S

A

G

M

W

H
|
H

V

M

H
|
H

Q

Q

S
|
S

I

L

L

V

S

D

K

K

E

D

T

T

G

R

L

K

N

N

I

L

F

F

S

I

N

P

P

S

D

E

G

G

S

E

E

V

S

L

E

S

A

P

F

L

F

G

H

R

-

A

Y

Y

I

A

G

G

L

L

Q

L

R

A

Y

N

I

G

P

S

A

A

M

S

A

S

L

F

V

T

A

T

P

P

Y

T

V

V

N

N

S

G

Y

Y

R

R

L

R

S

Q

R

P

D

Y

T

S

A

L

A

A

P

P

I

D

N

R

I

R

A

A

W

W

G

A

H

K

D

D

N

N

R
x
K

T

A

V

A

G

M

I

V

R
|
R

S

V

P

V

I

S

A

A

T

T

P

G

Q

D

-

P

A

A

R

S

R
|
R

V

I

E
|
E

N

N

R

R

V

I

I

G

G

E

A

P

D

G

A

A

N

N

P

P

Y

Y

V

L

A

Y

L

M

A

A

M

S

T

Q

L

I

A

V

C

S

G

G

Y

L

L

D

G

G

I

I

K

K

N

N

K

K

I

K

K

D

P

P

K

-

P

-

E

-

M

-

K

-

G

G

D

E

A

L

Y

Q

L

E

S

S

P

P

Y

Y

S

D

-

A

-

Q

-

V

-

P

-

M

L

L

P

P

R

T

S

T

L

L

G

A

E

E

A

A

L

L

D

D

W

A

L

L

R

E

R

H

E

D

S

S

D

E

L

L

-

F

H

R

E

S

V

C

L

F

G

G

R

D

E

T

F

F

V

I

T

K

V

Y

Y

W

S

L

E

Q

I

L

K

R

E

R

M

S

E

E

F

W

A

A

E

R

F

F

M

L

binding phosphoaminophosphonic acid-adenylate ester: G157 (≠ A146), E159 (= E148), R226 (≠ D208), F241 (= F223), V243 (≠ H225), H290 (= H271), S292 (= S273), K360 (≠ R340), R365 (= R345), R376 (= R355)
binding magnesium ion: E159 (= E148), E238 (= E220)
binding manganese (ii) ion: E159 (= E148), E161 (= E150), E231 (= E213), E238 (= E220), H288 (= H269), E378 (= E357)

8wwvA Glutamine synthetase
31% identity, 93% coverage: 19:444/459 of query aligns to 9:464/490 of 8wwvA

query
sites
8wwvA

W

W

L

T

N

N

E

E

R

Q

R

R

-

D

-

A

-

D

-

K

-

V

V

L

T

A

E

E

V

I

E

D

C

S

L

L

-

G

-

L

-

E

-

M

-

I

-

R

-

L

-

S

V

W

P

A

D

D

L

Q

T

Y

G

G

V

L

A

L

R

R

G

G

K

K

I

S

L

L

P

T

-

V

-

A

-

S

-

L

R

K

G

S

K

A

F

F

T

K

E

E

D

G

R

S

G

E

M

V

R

A

L

I

P

G

E

P

-

F

A

D

V

L

V

V

A

S

M

S

G

M

V

V

T

F

G

N

E

L

F

F

P

T

E

T

S

A

G

G

P

D

Y

F

Y

E

D

D

V

E

I

L

S

S

-

G

-

N

P

P

T

T

D

-

K

-

D

-

M

V

H

V

L

M

R

V

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P

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D

P

P

S

T

T

T

V

F

R

K

I

V

V

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P

P

W

W

A

A

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-

N

D

P

K

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T

A

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W

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A

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D

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A

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Y

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E

S

A

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E

E

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G

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Y

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F

V

K

V

C

A
x
G

P

I

E
|
E

L

L

E
|
E

F

W

Y

Y

L

L

-

T

-

K

-

I

V

V

A

D

R

R

N

S

T

L

D

S

P

P

N

E

T

S

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L

L

G

R

A

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P

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I

V

G

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D

G

A

-

I

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A

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T

P

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V

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A

Q

Q

A

G

Y

Y

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S

I

V

D

L

A

L

V

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N

H

E

H

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L

D

D

P

Q

L

V

F

-

E

D

D

D

I

I

Y

M

D

S

Y

K

C

V

E

R

K

K

-

G

-

L

M

L

E

E

L

L

N

N

V

L

-

P

-

L

D
x
R

T

S

L

I

I

E

H

D

E
|
E

V

W

G

A

A

P

G

S

Q

Q

M

M

E
|
E

I

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N

T

F
|
F

F
x
D

H
x
V

A

M

H

E

P

G

L

L

G

E

L

A

A

A

D

D

E

A

V

A

F

L

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I

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K

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T

A

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I

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A

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A

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N

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M

Y

Y

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A

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T

F

F

M

M

A

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K

K

P

P

-

A

I

I

A

N

G

G

E

F

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F

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x
A

S

S

A

G

M

W

H
|
H

V

M

H
|
H

Q

Q

S
|
S

I

L

L

V

S

D

K

K

E

D

T

T

G

R

L

K

N

N

I

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F

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P

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G

G

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A

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-

A

Y

Y

I

A

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G

L

L

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A

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N

N

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Y

Y

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|
R

L

R

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A

L

A

A

P

P

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N

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I

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K

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D

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N

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K

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A

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A

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A

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|
R

V

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|
E

N

N

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V

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I

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G

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A

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D

G

A

A

N

N

P

P

Y

Y

V

L

A

Y

L

M

A

A

M

S

T

Q

L

I

A

V

C

S

G

G

Y

L

L

D

G

G

I

I

K

K

N

N

K

K

I

K

K

D

P

P

K

-

P

-

E

-

M

-

K

-

G

G

D

E

A

L

Y

Q

L

E

S

S

P

P

Y

Y

S

D

-

A

-

Q

-

V

-

P

-

M

L

L

P

P

R

T

S

T

L

L

G

A

E

E

A

A

L

L

D

D

W

A

L

L

R

E

R

H

E

D

S

S

D

E

L

L

-

F

H

R

E

S

V

C

L

F

G

G

R

D

E

T

F

F

V

I

T

K

V

Y

Y

W

S

L

E

Q

I

L

K

R

E

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M

S

E

E

F

W

A

A

E

R

F

F

M

L

binding adenosine-5'-diphosphate: G155 (≠ A146), E157 (= E148), R224 (≠ D208), F239 (= F223), D240 (≠ F224), V241 (≠ H225), H288 (= H271), S290 (= S273), R374 (= R355), E376 (= E357)
binding magnesium ion: E157 (= E148), E236 (= E220)
binding manganese (ii) ion: E157 (= E148), E159 (= E150), E229 (= E213), E236 (= E220), H286 (= H269), E376 (= E357)
binding l-methionine-s-sulfoximine phosphate: E157 (= E148), E159 (= E150), E229 (= E213), E236 (= E220), A282 (≠ G265), H286 (= H269), R340 (= R322), K358 (≠ R340)

A0R083 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
31% identity, 82% coverage: 84:458/459 of query aligns to 64:446/446 of A0R083

query
sites
A0R083

D

E

K

S

D

D

M

T

H

V

L

A

R

R

P

P

D

D

P

P

S

S

T

T

V

F

R

Q

I

V

V

L

P

P

W

W

A

K

S

T

N

S

P

D

-

G

-

N

-

H

-

Y

T

S

A

A

Q

R

V

M

I

F

H

C

D

D

C

I

F

T

D

M

P

P

A

D

G

G

K

S

L

P

I

S

P

W

F

A

A

D

P

S

R

R

S

H

V

V

L

L

R

R

V

Q

C

L

E

A

L

K

Y

A

E

S

A

D

E

L

G

G

L

F

V

T

P

C

V

Y

V

V

A

H

P

P

E

E

L

I

E

E

F

F

Y

F

L

L

V

L

A

-

R

-

N

K

T

P

D

G

P

P

N

N

T

D

L

G

L

T

R

P

P

P

P

E

I

P

G

A

R

D

S

N

G

G

R

-

A

-

E

-

T

-

S

-

R

-

Q

-

A

G

Y

Y

S

F

I

D

D

Q

A

A

V

V

N

H

E

D

F

A

D

A

P

P

L

N

F

F

E

R

-

R

D

H

I

A

Y

I

D

E

Y

A

C

L

E

E

K

Q

M

M

E

G

L

I

N

S

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V

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E

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F

L

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I

H

H

H

E

E

V

G

G

A

A

P

G

G

Q

Q

M

Q

E

E

I

I

N

D

F

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F

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H

Y

A

A

H

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P

A

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M

A

A

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E

E

A

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A

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F

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A

A

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H

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Q

M

S

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I

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L

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K

G

E

D

T

T

G

-

L

-

N

N

I

A

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S

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G

D

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-

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A

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Y

F

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I

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Y

H

I

A

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N

A

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A

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A

A

L

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A

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Y

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Y

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K

R

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L

L

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V

R

H

D

G

T

G

A

E

A

A

P

P

I

T

N

A

I

A

A

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W

W

G

G

H

A

D

A

N

N

R

R

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A

G

L

I

V

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R

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V

P

P

I

M

A

Y

T

T

P

P

Q

H

-

K

-

V

-

S

A

S

R

R

V

V

E

E

N

V

R

R

V

S

I

P

G

D

A

S

D

A

A

C

N

N

P

P

Y

Y

V

L

A

T

L

F

A

A

M

V

T

L

L

L

A

A

C

A

G

G

Y

L

L

R

G

G

I

V

K

E

N
x
K

K

G

I

Y

K

V

P

L

K

G

P

P

E

Q

M

A

K

E

G

D

D

N

A

V

Y

W

-

S

-

L

-

T

-

Q

-

E

-

R

-

A

L

M

S

G

P

Y

Y

R

S

E

L

L

P

P

R

T

S

S

L

L

G

G

E

N

A

A

L

L

D

E

W

S

L

M

R

E

R

N

E

S

S

E

D

L

L

V

H

A

E

E

V

A

L

L

G

G

R

E

E

H

F

V

V

F

T

D

V

Y

Y

F

S

L

E

R

I

N

K

K

E

R

M

S

E

E

F

W

A

E

E

N

F

Y

M

R

K

S

V

H

I

V

S

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P

P

W

Y

E

E

R

L

E

K

H

N

L

Y

L

L

L

S

L

L

K363 (≠ N384) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

7tf6A Glutamine synthetase (see paper)
31% identity, 81% coverage: 84:456/459 of query aligns to 63:435/438 of 7tf6A

query
sites
7tf6A

D

E

K

S

D

D

M

M

H

Y

L

L

R

H

P

P

D

D

P

L

S

D

T

T

V

W

R

V

I

I

V

F

P

P

W

W

A

G

S

K

N

-

P

-

T

V

A

A

Q

R

V

L

I

I

H

C

D

D

C

V

F

Y

D

K

P

T

A

D

G

G

K

T

L

P

I

F

P

E

F

G

A

D

P

P

R

R

S

A

V

N

L

L

R

K

R

R

V

V

C

L

E

K

L

E

Y

M

E

E

A

D

E

L

G

G

L

F

V

T

P

D

V

F

-

N

V

L

A

G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

-

F

-

K

V

L

A

D

R

E

N

K

T

G

D

E

P

P

N

T

T

L

L

E

L

L

R

N

P

D

I

G

G

G

R

Y

S

F

G

D

R

L

A

A

E

P

T

T

S

D

R

L

Q

G

A

-

Y

-

S

-

I

-

D

-

A

-

V

-

N

-

E

-

F

-

D

E

P

N

L

C

F

R

E

R

D

D

I

I

Y

V

D

L

Y

E

C

L

E

E

K

D

M

M

E

G

L

F

N

D

V

I

D

E

T

A

L

S

I

H

H

H

E
|
E

V

V

G

A

A

P

G

G

Q

Q

M

H

E
|
E

I

I

N

D

F

F

F

K

H

Y

A

A

H

D

P

A

L

V

G

T

L

A

A

C

D

D

E

N

V

I

F

Q

F

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

A

I

A

A

L

R

R

K

H

H

N

N

M

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

G
|
G

E

V

P
x
N

G
|
G

S

S

A

G

M

M

H
|
H

V

F

H

N

Q

V

S

S

I

L

L

F

S

K

K

G

E

K

T

E

G

N

L

A

N

F

I

F

F

D

S

P

N

N

P

T

D

E

G

M

S

G

E

L

S

T

E

E

A

T

F

A

F

Y

H

Q

Y

F

I

T

G

A

G

G

L

V

Q

L

R

K

Y

N

I

A

P

R

A

G

A

F

M

T

A

A

L

V

V

C

A

N

P

P

Y

L

V

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

S

V

R

P

D
x
G

T
x
Y

A
x
E

A

A

P

P

I

C

N

Y

I

I

A

A

W

W

G

S

H

G

D

K

N

N

R
|
R

T

S

V

P

G

L

I

I

R

R

S

V

P

P

I

S

A

S

T

R

P

G

Q

L

A

S

R

T

R

R

V

I

E
|
E

N

V

R

R

V

S

I

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

V

M

A

A

L

L

A

A

M

A

T

I

L

L

A

E

C

A

G

G

Y

L

L

D

G

G

I

I

K

K

N

N

K

K

I

L

K

K

-

V

P

P

K

E

P

P

-

V

-

N

-

Q

-

N

-

I

-
x
Y

E

E

M

M

K

N

G

R

D

E

A

E

Y

R

-

E

-

A

L

V

S

G

P

I

Y

Q

S

D

L

L

P

P

R

S

S

T

L

L

G

Y

E

T

A

A

L

L

D

K

W

A

L

M

R

R

R

E

E

N

S

E

D

V

L

I

H

K

E

K

V

A

L

L

G

G

R

N

E

H

F

I

V

Y

T

N

V

Q

Y

F

S

I

E

N

I

S

K

K

E

S

M

I

E

E

F

W

A

D

E
x
Y

F

Y

M

R

K

T

V

Q

I

V

S

S

P

E

W

W

E

E

R

R

E

D

H
x
Q

L

Y

L

M

binding glutamine: E128 (= E150), E183 (= E213), G235 (= G265), H239 (= H269), R292 (= R322), E298 (≠ A328)
binding magnesium ion: E126 (= E148), E128 (= E150), E183 (= E213), E190 (= E220), H239 (= H269), E327 (= E357)
binding : G232 (= G262), N234 (≠ P264), G296 (≠ D326), Y297 (≠ T327), R310 (= R340), Y367 (vs. gap), Y421 (≠ E442), Q433 (≠ H454)

Sites not aligning to the query:

binding : 58, 60, 437

Query Sequence

>WP_011802240.1 NCBI__GCF_000015505.1:WP_011802240.1
MSFNMSMSKASTFTDLENWLNERRVTEVECLVPDLTGVARGKILPRGKFTEDRGMRLPEA
VVAMGVTGEFPESGPYYDVISPTDKDMHLRPDPSTVRIVPWASNPTAQVIHDCFDPAGKL
IPFAPRSVLRRVCELYEAEGLVPVVAPELEFYLVARNTDPNTLLRPPIGRSGRAETSRQA
YSIDAVNEFDPLFEDIYDYCEKMELNVDTLIHEVGAGQMEINFFHAHPLGLADEVFFFKR
TVREAALRHNMYATFMAKPIAGEPGSAMHVHQSILSKETGLNIFSNPDGSESEAFFHYIG
GLQRYIPAAMALVAPYVNSYRRLSRDTAAPINIAWGHDNRTVGIRSPIATPQARRVENRV
IGADANPYVALAMTLACGYLGIKNKIKPKPEMKGDAYLSPYSLPRSLGEALDWLRRESDL
HEVLGREFVTVYSEIKEMEFAEFMKVISPWEREHLLLLV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory