SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011802541.1 NCBI__GCF_000015505.1:WP_011802541.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P16703 Cysteine synthase B; CSase B; O-acetylserine (thiol)-lyase B; OAS-TL B; O-acetylserine sulfhydrylase B; EC 2.5.1.47 from Escherichia coli (strain K12) (see paper)
63% identity, 99% coverage: 3:298/300 of query aligns to 1:292/303 of P16703

query
sites
P16703

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N71 (= N77) binding pyridoxal 5'-phosphate
S255 (= S261) binding pyridoxal 5'-phosphate

2bhtA Crystal structure of o-acetylserine sulfhydrylase b (see paper)
62% identity, 99% coverage: 3:298/300 of query aligns to 1:292/294 of 2bhtA

query
sites
2bhtA

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active site: K41 (= K47), S69 (= S75), Q199 (= Q205), G203 (= G209), S255 (= S261), C280 (= C286)
binding pyridoxal-5'-phosphate: K41 (= K47), N71 (= N77), M173 (= M179), G174 (= G180), T175 (= T181), T176 (= T182), T178 (= T184), G208 (= G214), S255 (= S261), C280 (= C286)

2efyA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-acetylbutyric acid
44% identity, 98% coverage: 6:298/300 of query aligns to 3:301/302 of 2efyA

query
sites
2efyA

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active site: K40 (= K47), S70 (= S75), E200 (≠ Q205), S204 (≠ G209), S263 (= S261)
binding 5-oxohexanoic acid: T69 (= T74), G71 (= G76), T73 (= T78), Q141 (= Q146), G175 (= G180), G219 (≠ K217), M220 (≠ W218), P222 (≠ Q220)
binding pyridoxal-5'-phosphate: K40 (= K47), N72 (= N77), Y172 (≠ S177), G175 (= G180), T176 (= T181), G177 (≠ T182), T179 (= T184), G219 (≠ K217), S263 (= S261), P289 (vs. gap), D290 (= D287)

2ecqA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 3-hydroxylactate
44% identity, 98% coverage: 6:298/300 of query aligns to 3:301/302 of 2ecqA

query
sites
2ecqA

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active site: K40 (= K47), S70 (= S75), E200 (≠ Q205), S204 (≠ G209), S263 (= S261)
binding (3s)-3-hydroxybutanoic acid: K40 (= K47), G71 (= G76), T73 (= T78), Q141 (= Q146), G219 (≠ K217)
binding pyridoxal-5'-phosphate: K40 (= K47), N72 (= N77), Y172 (≠ S177), G173 (≠ A178), G175 (= G180), T176 (= T181), T179 (= T184), G219 (≠ K217), S263 (= S261), P289 (vs. gap)

2ecoA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-methylvalerate
44% identity, 98% coverage: 6:298/300 of query aligns to 3:301/302 of 2ecoA

query
sites
2ecoA

I

V

E

E

D

G

A

A

V

I

G

G

K

K

T

T

P

P

L

V

V

V

A

R

L

L

Q

A

R

K

I

V

A

V

A

E

A

P

D

D

N

M

A

A

A

E

R

-

G

-

N

-

I

-

L

V

L

W

A

V

K

K

L

L

E

E

G

G

N

L

N

N

P

P

A

G

G

G

S

S

V

I

K
|
K

D

D

R

R

P

P

A

A

L

W

S

Y

M

M

I

I

R

K

R

D

A

A

E

E

R

R

G

G

D

I

I

L

K

R

P

P

G

G

D

S

-

G

-

Q

T

V

L

I

I

V

E

E

A

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

G

L

L

A

A

M

M

A

I

A

A

I

S

K

R

G

G

Y

Y

R

R

M

L

I

I

L

L

I

T

M

M

P

P

E

A

D

Q

L

M

S

S

I

E

E

E

R

R

A

K

Q

R

T

V

M

L

K

K

A

A

F

F

G

G

A

A

E

E

L

L

I

V

L

L

T

T

P

D

K

P

S

E

A

R

G

R

M

M

E

L

Y

A

C

A

R

R

D

E

L

E

A

A

D

L

R

R

M

L

Q

K

Q

E

E

E

G

L

K

G

G

A

R

F

V

M

L

P

D

D

Q
|
Q

F

F

A

K

N

N

P

P

D

A

N

N

P

V

R

R

I

A

H

H

Y

Y

E

E

T

T

G

G

P

P

E

E

I

L

W

Y

T

E

D

A

T

L

F

E

G

G

K

R

I

I

T

D

H

A

F

F

V

V

S
x
Y

A

G

M

S

G
|
G

T
|
T

T

G

G

G

T
|
T

I

I

T

T

G

G

V

V

S

G

R

R

Y

Y

L

L

K

K

E

E

Q

R

N

I

P

P

A

H

I

V

Q

K

I

V

I

I

G

A

A

V

Q
x
E

P

P

S

A

E

R

G
x
S

S

N

R

V

I

L

P

S

-

G

-

K

-

M

-

G

-

Q

-

H

G

G

I

F

R

Q

K
x
G

W

M

P

G

Q

P

E

G

Y

F

L

I

P

P

K

E

I

N

Y

L

D

D

P

L

S

S

A

L

V

L

D

D

E

G

L

V

V

I

L

Q

V

V

S

W

Q

E

L

E

D

D

A

A

E

F

D

P

M

L

C

A

R

R

M

L

A

A

R

R

E

E

G

G

I

L

F

F

A

L

G

G

I

M

S
|
S

A

S

A

G

G

G

A

I

C

V

W

W

V

A

A

A

Q

L

E

Q

I

V

A

A

K

R

T

E

V

L

Q

G

D

P

A

G

V

K

I

R

V

V

F

A

V

C

V

I

C

S

-
x
P

D
|
D

R

G

G

G

D

W

R

K

Y

Y

L

L

S

S

T

T

G

P

V

L

F

Y

active site: K40 (= K47), S70 (= S75), E200 (≠ Q205), S204 (≠ G209), S263 (= S261)
binding 4-methyl valeric acid: K40 (= K47), T69 (= T74), G71 (= G76), T73 (= T78), Q141 (= Q146), G175 (= G180), T176 (= T181), G219 (≠ K217)
binding pyridoxal-5'-phosphate: K40 (= K47), N72 (= N77), Y172 (≠ S177), G175 (= G180), T176 (= T181), T179 (= T184), G219 (≠ K217), S263 (= S261), P289 (vs. gap), D290 (= D287)

4lmbA Crystal structure analysis of o-acetylserine sulfhydrylase cysk2 complexed with cystine from microcystis aeruginosa 7806 (see paper)
44% identity, 98% coverage: 6:298/300 of query aligns to 7:308/310 of 4lmbA

query
sites
4lmbA

I

I

E

T

D

Q

A

L

V

V

G

G

K

R

T

T

P

P

L

L

V

V

A

Q

L

L

Q

N

R

R

I

I

A

P

A

V

A

A

D

E

N

G

A

V

-

K

A

A

R

R

G

-

N

-

I

-

L

I

L

V

A

V

K

K

L

L

E

E

G

S

N

M

N

N

P

P

A

A

G

A

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

S

S

M

M

I

V

R

E

R

D

A

A

E

E

E

A

R

A

G

G

D

L

I

I

K

H

P

P

G

D

D

K

T

T

-

I

L

L

I

V

E

E

A

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

M

M

A

V

A

A

I

A

K

K

G

G

Y

Y

R

R

M

L

I

V

L

L

I

T

M

M

P

P

E

E

D

T

L
x
M

S

S

I

L

E

E

R

R

A

R

Q

A

T

M

M

L

K

K

A

A

F

Y

G

G

A

A

E

Q

L

L

I

E

L

L

T

T

P

P

K

G

S

S

A

Q

G

G

M
|
M

E

E

Y

G

C

A

R

I

D

T

L

R

A

A

D

E

R

E

M

I

-

V

Q

E

Q

N

E

T

G

P

K

N

G

A

R

Y

V

S

L

L

D

Q

Q
|
Q

F
|
F

A

R

N

N

P

P

D

A

N

N

P

P

R

K

I

I

H

H

Y

R

E

E

T

T

G

A

P

E

E

E

I

I

W

W

T

A

D

D

T

T

F

D

G

G

K

L

I

V

T

D

H

I

F

V

V

I

S

G

A

G

M
x
V

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

T

T

G

G

V

I

S

A

R

E

Y

T

L

I

K

K

E

P

Q

R

N

R

P

P

A

Q

I

F

Q

Q

I

A

I

I

G

A

A

V

Q

E

P

P

S

S

E

N

G

S

S

P

R

V

I

L

P

S

G

G

I

G

R

Q

K

P

W

G

P

P

Q

H

E

K

-

I

-
x
Q

-
x
G

-
x
I

-

G

-
x
A

-

G

Y

F

L

I

P

P

K

A

I

I

Y

F

D

R

P

P

S

E

A

L

V

I

D

D

E

E

L

V

V

I

L

I

V

V

S

D

Q

D

L

T

D

E

A

A

E

F

D

A

M

Y

C

A

R

R

M

L

A

A

R

R

E

Q

E

E

G

G

I

L

F

L

A

S

G

G

I

I

S
|
S

A

A

A

G

G

A

A

A

C

L

W

W

V

A

A

A

Q

I

E

Q

I

V

A

G

K

K

-

R

-

P

T

E

V

N

Q

E

D

D

A

K

V

L

I

I

V

V

F

M

V

I

V

Q

C
x
P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

A

V

L

F

F

active site: K46 (= K47), S269 (= S261)
binding cysteine: K46 (= K47), T74 (= T74), S75 (= S75), N77 (= N77), T78 (= T78), M101 (≠ L101), M125 (= M125), M125 (= M125), Q147 (= Q146), F148 (= F147), Q224 (vs. gap), G225 (vs. gap), G225 (vs. gap), I226 (vs. gap), A228 (vs. gap)
binding pyridoxal-5'-phosphate: K46 (= K47), N77 (= N77), V180 (≠ M179), G181 (= G180), T182 (= T181), G183 (≠ T182), T185 (= T184), G225 (vs. gap), S269 (= S261), P296 (≠ C286)

4aecA Crystal structure of the arabidopsis thaliana o-acetyl-serine-(thiol)- lyasE C (see paper)
44% identity, 98% coverage: 5:298/300 of query aligns to 16:316/323 of 4aecA

query
sites
4aecA

T

N

I

V

E

S

D

Q

A

L

V

I

G

G

K

K

T

T

P

P

L

M

V

V

A

Y

L

L

Q

N

R

S

I

I

A

A

A

K

A

G

D

C

N

V

A

A

A

N

R

-

G

-

N

-

I

-

L

I

L

A

A

A

K

K

L

L

E

E

G

I

N

M

N

E

P

P

A

C

G

C

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

Y

S

S

M

M

I

V

R

T

R

D

A

A

E

E

E

Q

R

K

G

G

D

F

I

I

K

S

P

P

G

G

D

K

T

S

-

V

L

L

I

V

E

E

A

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

G

L

L

A

A

M

F

A

I

A

A

I

S

K

R

G

G

Y

Y

R

R

M

L

I

I

L

L

I

T

M

M

P

P

E

A

D

S

L

M

S

S

I

M

E

E

R

R

A

R

Q

V

T

L

M

L

K

K

A

A

F

F

G

G

A

A

E

E

L

L

I

V

L

L

T

T

P

D

K

P

S

A

A

K

G

G

M

M

E

T

Y

G

C

A

R

V

D

Q

L

K

A

A

D

E

R

E

-

I

M

L

Q

K

Q

N

E

T

G

P

K

D

G

A

R

Y

V

M

L

L

D

Q

Q

Q

F

F

A

D

N

N

P

P

D

A

N

N

P

P

R

K

I

I

H

H

Y

Y

E

E

T

T

G

G

P

P

E

E

I

I

W

W

T

D

D

D

T

T

F

K

G

G

K

K

I

V

T

D

H

I

F

F

V

V

S

A

A

G

M
x
I

G
|
G

T
|
T

T
x
G

G
|
G

T
|
T

I

I

T

T

G

G

V

V

S

G

R

R

Y

F

L

I

K

K

E

E

Q

K

N

N

P

P

A

K

I

T

Q

Q

I

V

I

I

G

G

A

V

Q

E

P

P

S

T

E

E

G

S

S

D

R

I

I

L

P

S

G

G

I

G

R

K

K

P

W

G

P

P

Q

H

E

K

-

I

-

Q

-
x
G

-

I

-

G

-

A

-

G

Y

F

L

I

P

P

K

K

I

N

Y

L

D

D

P

Q

S

K

A

I

V

M

D

D

E

E

L

V

V

I

L

A

V

I

S

S

Q

S

L

E

D

E

A

A

E

I

D

E

M

T

C

A

R

K

R

Q

M

L

A

A

R

L

E

K

E

E

G

G

I

L

F

M

A

V

G

G

I

I

S
|
S

A

S

A

G

G

A

A

A

C

A

W

A

V

A

A

A

Q

I

E

K

I

V

A

A

K

K

T

R

V

P

Q

E

D

N

A

A

-

G

-

K

V

L

I

I

V

A

F

V

V

V

V

F

C
x
P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

P

V

L

F

F

active site: K54 (= K47), S277 (= S261)
binding pyridoxal-5'-phosphate: K54 (= K47), N85 (= N77), I188 (≠ M179), G189 (= G180), T190 (= T181), G191 (≠ T182), G192 (= G183), T193 (= T184), G233 (vs. gap), S277 (= S261), P304 (≠ C286)

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
43% identity, 99% coverage: 4:300/300 of query aligns to 5:308/320 of 2isqA

query
sites
2isqA

Q

K

T

D

I

V

E

T

D

E

A

L

V

I

G

G

K

N

T

T

P

P

L

L

V

V

A

Y

L

L

Q

-

R

-

I

-

A

-

D

N

N

N

A

V

A

A

R

E

G

G

N

C

I

V

-

G

-

R

L

V

L

A

A

A

K

K

L

L

E

E

G

M

N

M

N

E

P

P

A

C

G

S

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

F

S

S

M

M

I

I

R

S

R

D

A

A

E

E

E

K

R

K

G

G

D

L

I

I

K

K

P

P

G

G

D

E

T

S

-

V

L

L

I

I

E

E

A

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

M

F

A

T

A

A

I

A

K

K

G

G

Y

Y

R

K

M

L

I

I

L

I

I

T

M

M

P

P

E

A

D

S

L

M

S

S

I

T

E

E

R

R

A

R

Q

I

T

I

M

L

K

L

A

A

F

F

G

G

A

V

E

E

L

L

I

V

L

L

T

T

P

D

K

P

S

A

A

K

G
|
G

M
|
M

E
x
K

Y

G

C

A

R

I

D

A

L

K

A

A

D

E

R

E

-

I

M

L

Q

A

Q

K

E

T

G

P

K

N

G

G

R

Y

V

M

L

L

D

Q

Q

Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

R

K

I

I

H

H

Y

Y

E

E

T

T

G

G

P

P

E

E

I

I

W

W

T

K

D

G

T

T

F

G

G

G

K

K

I

I

T

D

H

G

F

F

V

V

S

S

A
x
G

M

I

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

T

T

G

G

V

A

S

G

R

K

Y

Y

L

L

K

K

E

E

Q

Q

N

N

P

A

A

N

I

V

Q

K

I

L

I

Y

G

G

A

V

Q

E

P

P

S

V

E

E

G

S

S

A

R

I

I

L

P

S

G

G

I

G

R

K

K

P

W
x
G

P
|
P

Q
x
H

E

K

-

I

-
x
Q

-
x
G

-

I

-

G

-

A

-

G

Y

F

L

I

P

P

K

S

I

V

Y

L

D

N

P

V

S

D

A

L

V

I

D

D

E

E

L

V

V

V

L

Q

V

V

S

S

Q

S

L

D

D

E

A

S

E

I

D

D

M

M

C

A

R

R

R

Q

M

L

A

A

R

L

E

K

E

E

G

G

I

L

F

L

A

V

G

G

I

I

S
|
S

A

S

A

G

G

A

A

A

C

A

W

A

V

A

A

A

Q

I

E

K

I

L

A

A

K

Q

T

R

V

P

Q

E

D

N

A

A

V

G

I

K

V

L

F

F

V

V

V

A

C

I

-

F

-
x
P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

V

V

L

F

F

P

D

A

A

active site: K44 (= K47), S267 (= S261)
binding pyridoxal-5'-phosphate: K44 (= K47), N75 (= N77), G177 (≠ A178), G179 (= G180), T180 (= T181), G181 (≠ T182), T183 (= T184), G223 (vs. gap), S267 (= S261), P294 (vs. gap)
binding : T72 (= T74), S73 (= S75), G74 (= G76), T76 (= T78), G122 (= G124), M123 (= M125), K124 (≠ E126), G217 (≠ W218), P218 (= P219), H219 (≠ Q220), Q222 (vs. gap), G223 (vs. gap)

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
43% identity, 99% coverage: 4:300/300 of query aligns to 7:310/322 of P47998

query
sites
P47998

Q

K

T

D

I

V

E

T

D

E

A

L

V

I

G

G

K

N

T

T

P

P

L

L

V

V

A

Y

L

L

Q

-

R

-

I

-

A

-

D

N

N

N

A

V

A

A

R

E

G

G

N

C

I

V

-

G

-

R

L

V

L

A

A

A

K

K

L

L

E

E

G

M

N

M

N

E

P

P

A

C

G

S

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

F

S

S

M

M

I

I

R

S

R

D

A

A

E

E

E

K

R

K

G

G

D

L

I

I

K

K

P

P

G

G

D

E

T

S

-

V

L

L

I

I

E

E

A

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

M

F

A

T

A

A

I

A

K

K

G

G

Y

Y

R

K

M

L

I

I

L

I

I

T

M

M

P

P

E

A

D

S

L

M

S

S

I

T

E

E

R

R

A

R

Q

I

T

I

M

L

K

L

A

A

F

F

G

G

A

V

E

E

L

L

I

V

L

L

T

T

P

D

K

P

S

A

A

K

G

G

M

M

E

K

Y

G

C

A

R

I

D

A

L

K

A

A

D

E

R

E

-

I

M

L

Q

A

Q

K

E

T

G

P

K

N

G

G

R

Y

V

M

L

L

D

Q

Q
|
Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

R

K

I

I

H
|
H

Y

Y

E

E

T

T

G
|
G

P

P

E

E

I

I

W

W

T

K

D

G

T

T

F

G

G

G

K

K

I

I

T

D

H

G

F

F

V

V

S

S

A

G

M

I

G
|
G

T
|
T

T
x
G

G
|
G

T
|
T

I

I

T

T

G

G

V

A

S

G

R

K

Y

Y

L

L

K

K

E

E

Q

Q

N

N

P

A

A

N

I

V

Q

K

I

L

I

Y

G

G

A

V

Q

E

P

P

S

V

E

E

G

S

S

A

R

I

I

L

P

S

G

G

I

G

R
x
K

K

P

W

G

P

P

Q
x
H

E
x
K

-

I

-

Q

-

G

-

I

-

G

-

A

-

G

Y

F

L

I

P

P

K

S

I

V

Y

L

D

N

P

V

S

D

A

L

V

I

D

D

E

E

L

V

V

V

L

Q

V

V

S

S

Q

S

L

D

D

E

A

S

E

I

D

D

M

M

C

A

R

R

R

Q

M

L

A

A

R

L

E

K

E

E

G

G

I

L

F

L

A

V

G

G

I

I

S
|
S

A

S

A

G

G

A

A

A

C

A

W

A

V

A

A

A

Q

I

E

K

I

L

A

A

K

Q

T

R

V

P

Q

E

D

N

A

A

V

G

I

K

V

L

F

F

V

V

V

A

C

I

-

F

-

P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

V

V

L

F

F

P

D

A

A

K46 (= K47) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T74) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S75) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N77) binding pyridoxal 5'-phosphate; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T78) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q146) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H156) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (= G161) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (≠ GTTGT 180:184) binding pyridoxal 5'-phosphate
T182 (= T181) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (= T184) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (≠ R216) mutation to A: Impaired interaction with SAT1.
H221 (≠ Q220) mutation to A: Impaired interaction with SAT1.
K222 (≠ E221) mutation to A: Impaired interaction with SAT1.
S269 (= S261) binding pyridoxal 5'-phosphate; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
43% identity, 98% coverage: 4:298/300 of query aligns to 5:305/306 of 2q3dA

query
sites
2q3dA

Q

E

T

D

I

I

E

T

D

Q

A

L

V

I

G

G

K

R

T

T

P

P

L

L

V

V

A

R

L

L

Q

R

R

R

I

V

-

T

-

D

-

G

A

A

A

V

A

A

D

D

N

-

A

-

R

-

G

-

N

-

I

-

L

I

L

V

A

A

K

K

L

L

E

E

G

F

N

F

N

N

P

P

A

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

S

A

M

M

I

L

R

Q

R

A

A

A

E

E

E

Q

R

A

G

G

D

L

I

I

K

K

P

P

G

D

D

T

T

I

L

I

I

L

E

E

A

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

M

M

A

V

A

C

A

A

I

A

K

R

G

G

Y

Y

R

R

M

C

I

V

L

L

I

T

M

M

P

P

E

E

D

T

L

M

S

S

I

L

E

E

R

R

A

R

Q

M

T

L

M

L

K

R

A

A

F

Y

G

G

A

A

E

E

L

L

I

I

L

L

T

T

P

P

K

G

S

A

A

D

G

G

M

M

E

S

Y

G

C

A

R

I

D

A

L

K

A

A

D

E

R

E

M

L

-

A

Q

K

Q

T

E

D

G

Q

K

R

G

Y

R

F

V

V

L

P

D

Q

Q
|
Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

R

A

I

I

H

H

Y

R

E

V

T

T

G

A

P

E

E

E

I

V

W

W

T

R

D

D

T

T

F

D

G

G

K

K

I

V

T

D

H

I

F

V

V

V

S

A

A

G

M
x
V

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

T

T

G

G

V

V

S

A

R

Q

Y

V

L

I

K

K

E

E

Q

R

N

K

P

P

A

S

I

A

Q

R

I

F

I

V

G

A

A

V

Q

E

P

P

S

A

E

A

G

S

S

P

R

V

I

L

P

S

G

G

I

G

R

Q

K

K

W

G

P

P

Q

H

E

P

-

I

-

Q

-
x
G

-
x
I

-

G

-

A

-

G

Y

F

L

V

P

P

K

P

I

V

Y

L

D

D

P

Q

S

D

A

L

V

V

D

D

E

E

L

I

V

I

L

T

V

V

S

G

Q

N

L

E

D

D

A

A

E

L

D

N

M

V

C

A

R

R

M

L

A

A

R

R

E

E

G

G

I

L

F

L

A

V

G

G

I

I

S
|
S

A

S

A

G

G

A

A

A

C

T

W

V

V

A

A

A

Q

L

E

Q

I

V

A

A

K

R

T

R

V

P

Q

E

D

N

A

A

-

G

-

K

V

L

I

I

V

V

F

V

V

V

V

L

C
x
P

D
|
D

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

P

V

L

F

F

active site: K44 (= K47), S266 (= S261), P293 (≠ C286)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K47), T71 (= T74), S72 (= S75), N74 (= N77), T75 (= T78), Q144 (= Q146), V177 (≠ M179), G178 (= G180), T179 (= T181), G180 (≠ T182), T182 (= T184), G222 (vs. gap), I223 (vs. gap), S266 (= S261), P293 (≠ C286), D294 (= D287)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
43% identity, 98% coverage: 4:298/300 of query aligns to 5:305/310 of P9WP55

query
sites
P9WP55

Q

E

T

D

I

I

E

T

D

Q

A

L

V

I

G

G

K

R

T

T

P

P

L

L

V

V

A

R

L

L

Q

R

R

R

I

V

-

T

-

D

-

G

A

A

A

V

A

A

D

D

N

-

A

-

R

-

G

-

N

-

I

-

L

I

L

V

A

A

K

K

L

L

E

E

G

F

N

F

N

N

P

P

A

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

S

A

M

M

I

L

R

Q

R

A

A

A

E

E

E

Q

R

A

G

G

D

L

I

I

K

K

P

P

G

D

D

T

T

I

L

I

I

L

E

E

A

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

M

A

V

A

C

A

A

I

A

K

R

G

G

Y

Y

R

R

M

C

I

V

L

L

I

T

M

M

P

P

E

E

D

T

L

M

S

S

I

L

E

E

R

R

A

R

Q

M

T

L

M

L

K

R

A

A

F

Y

G

G

A

A

E

E

L

L

I

I

L

L

T

T

P

P

K

G

S

A

A

D

G

G

M

M

E

S

Y

G

C

A

R

I

D

A

L

K

A

A

D

E

R

E

M

L

-

A

Q

K

Q

T

E

D

G

Q

K

R

G

Y

R

F

V

V

L

P

D

Q

Q

Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

R

A

I

I

H

H

Y

R

E

V

T

T

G

A

P

E

E

E

I

V

W

W

T

R

D

D

T

T

F

D

G

G

K

K

I

V

T

D

H

I

F

V

V

V

S

A

A

G

M

V

G
|
G

T
|
T

T
x
G

G
|
G

T
|
T

I

I

T

T

G

G

V

V

S

A

R

Q

Y

V

L

I

K

K

E

E

Q

R

N

K

P

P

A

S

I

A

Q

R

I

F

I

V

G

A

A

V

Q

E

P

P

S

A

E

A

G

S

S

P

R

V

I

L

P

S

G

G

I

G

R

Q

K

K

W

G

P

P

Q

H

E

P

-

I

-

Q

-

G

-

I

-

G

-

A

-

G

Y

F

L

V

P

P

K

P

I

V

Y

L

D

D

P

Q

S

D

A

L

V

V

D

D

E

E

L

I

V

I

L

T

V

V

S

G

Q

N

L

E

D

D

A

A

E

L

D

N

M

V

C

A

R

R

M

L

A

A

R

R

E

E

G

G

I

L

F

L

A

V

G

G

I

I

S
|
S

A

S

A

G

G

A

A

A

C

T

W

V

V

A

A

A

Q

L

E

Q

I

V

A

A

K

R

T

R

V

P

Q

E

D

N

A

A

-

G

-

K

V

L

I

I

V

V

F

V

V

V

V

L

C

P

D

D

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

P

V

L

F

F

K44 (= K47) modified: N6-(pyridoxal phosphate)lysine
N74 (= N77) binding pyridoxal 5'-phosphate
GTGGT 178:182 (≠ GTTGT 180:184) binding pyridoxal 5'-phosphate
S266 (= S261) binding pyridoxal 5'-phosphate

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
44% identity, 96% coverage: 10:298/300 of query aligns to 83:378/392 of P47999

query
sites
P47999

V

I

G

G

K

K

T

T

P

P

L

M

V

V

A

Y

L

L

Q

-

R

-

I

-

A

-

D

N

N

N

A

V

R

K

G

G

N

C

I

V

-

A

-

S

L

V

L

A

A

A

K

K

L

L

E

E

G

I

N

M

N

E

P

P

A

C

G

C

S

S

V

V

K

K

D

D

R

R

P

I

A

G

L

Y

S

S

M

M

I

I

R

T

R

D

A

A

E

E

R

K

G

G

D

L

I

I

K

T

P

P

G

G

D

K

T

S

-

V

L

L

I

V

E

E

A

S

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

M

F

A

I

A

A

I

S

K

K

G

G

Y

Y

R

K

M

L

I

I

L

L

I

T

M

M

P

P

E

A

D

S

L

M

S

S

I

L

E

E

R

R

A

R

Q

V

T

L

M

L

K

R

A

A

F

F

G

G

A

A

E

E

L

L

I

V

L

L

T

T

P

E

K

P

S

A

A

K

G

G

M

M

E

T

Y

G

C

A

R

I

D

Q

L

K

A

A

D

E

R

E

-

I

M

L

Q

K

E

T

G

P

K

N

G

S

R

Y

V

M

L

L

D

Q

Q

Q

F

F

A

D

N

N

P

P

D

A

N

N

P

P

R

K

I

I

H

H

Y

Y

E

E

T

T

G

G

P

P

E

E

I

I

W

W

T

E

D

D

T

T

F

R

G

G

K

K

I

I

T

D

H

I

F

L

V

V

S

A

A

G

M

I

G

G

T

T

T

G

G

G

T

T

I

I

T

T

G

G

V

V

S

G

R

R

Y

F

L

I

K

K

E

E

Q

R

N

K

P

P

A

E

I

L

Q

K

I

V

I

I

G

G

A

V

Q

E

P

P

S

T

E

E

G

S

S

A

R

I

I

L

P

S

G

G

I

G

R

K

K

P

W

G

P

P

Q

H

E

K

-

I

-

Q

-

G

-

I

-

G

-

A

-

G

Y

F

L

V

P

P

K

K

I

N

Y

L

D

D

P

L

S

A

A

I

V

V

D

D

E

E

L

Y

V

I

L

A

V

I

S

S

Q

S

L

E

D

E

A

A

E

I

D

E

M

T

C

S

R

K

R

Q

M

L

A

A

R

L

E

Q

E

E

G

G

I

L

F

L

A

V

G

G

I

I

S

S

A

S

A

G

G

A

A

A

C

A

W

A

V

A

A

A

Q

I

E

Q

I

V

A

A

K

K

T

R

V

P

Q

E

D

N

A

A

-

G

-

K

V

L

I

I

V

A

F

V

V

V

V

F

C

P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

Q

V

L

F

F

Sites not aligning to the query:

61 modified: N-acetylalanine

1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
43% identity, 99% coverage: 4:300/300 of query aligns to 5:308/320 of 1z7yA

query
sites
1z7yA

Q

K

T

D

I

V

E

T

D

E

A

L

V

I

G

G

K

N

T

T

P

P

L

L

V

V

A

Y

L

L

Q

-

R

-

I

-

A

-

D

N

N

N

A

V

A

A

R

E

G

G

N

C

I

V

-

G

-

R

L

V

L

A

A

A

K

K

L

L

E

E

G

M

N

M

N

E

P

P

A

C

G

S

S

S

V

V

K
x
A

D

D

R

R

P

I

A

G

L

F

S

S

M

M

I

I

R

S

R

D

A

A

E

E

E

K

R

K

G

G

D

L

I

I

K

K

P

P

G

G

D

E

T

S

-

V

L

L

I

I

E

E

A

P

T

T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

M

F

A

T

A

A

I

A

K

K

G

G

Y

Y

R

K

M

L

I

I

L

I

I

T

M

M

P

P

E

A

D

S

L

M

S

S

I

T

E

E

R

R

A

R

Q

I

T

I

M

L

K

L

A

A

F

F

G

G

A

V

E

E

L

L

I

V

L

L

T

T

P

D

K

P

S

A

A

K

G

G

M

M

E

K

Y

G

C

A

R

I

D

A

L

K

A

A

D

E

R

E

-

I

M

L

Q

A

Q

K

E

T

G

P

K

N

G

G

R

Y

V

M

L

L

D

Q

Q
|
Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

R

K

I

I

H

H

Y

Y

E

E

T

T

G

G

P

P

E

E

I

I

W

W

T

K

D

G

T

T

F

G

G

G

K

K

I

I

T

D

H

G

F

F

V

V

S

S

A

G

M
x
I

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

T

T

G

G

V

A

S

G

R

K

Y

Y

L

L

K

K

E

E

Q

Q

N

N

P

A

A

N

I

V

Q

K

I

L

I

Y

G

G

A

V

Q

E

P

P

S

V

E

E

G

S

S

A

R

I

I

L

P

S

G

G

I

G

R

K

K

P

W

G

P

P

Q

H

E

K

-

I

-

Q

-
x
G

-

I

-

G

-

A

-

G

Y

F

L

I

P

P

K

S

I

V

Y

L

D

N

P

V

S

D

A

L

V

I

D

D

E

E

L

V

V

V

L

Q

V

V

S

S

Q

S

L

D

D

E

A

S

E

I

D

D

M

M

C

A

R

R

R

Q

M

L

A

A

R

L

E

K

E

E

G

G

I

L

F

L

A

V

G

G

I

I

S
|
S

A

S

A

G

G

A

A

A

C

A

W

A

V

A

A

A

Q

I

E

K

I

L

A

A

K

Q

T

R

V

P

Q

E

D

N

A

A

V

G

I

K

V

L

F

F

V

V

V

A

C

I

-

F

-
x
P

D
x
S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

V

V

L

F

F

P

D

A

A

active site: A44 (≠ K47), S267 (= S261)
binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: G74 (= G76), N75 (= N77), T76 (= T78), Q145 (= Q146), I178 (≠ M179), G179 (= G180), T180 (= T181), G181 (≠ T182), T183 (= T184), G223 (vs. gap), S267 (= S261), P294 (vs. gap), S295 (≠ D287)

7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
43% identity, 98% coverage: 4:298/300 of query aligns to 5:306/309 of 7n2tA

query
sites
7n2tA

Q

K

T

D

I

V

E

T

D

E

A

L

V

I

G

G

K

N

T

T

P

P

L

L

V

V

A

Y

L

L

Q

N

R

R

I

V

A

V

A

D

A

G

D

-

N

C

A

V

A

A

R

R

G

-

N

-

I

-

L

V

L

A

A

A

K

K

L

L

E

E

G

M

N

M

N

E

P

P

A

C

G

S

S

S

V

V

K

K

D

D

R

R

P

I

A

G

L

Y

S

S

M

M

I

I

R

S

R

D

A

A

E

E

E

N

R

K

G

G

D

L

I

I

K

T

P

P

G

G

D

E

T

S

-

V

L

L

I

I

E

E

A

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

M

F

A

I

A

A

I

A

K

K

G

G

Y

Y

R

R

M

L

I

I

L

I

I

C

M

M

P

P

E

A

D

S

L

M

S

S

I

L

E

E

R

R

A

R

Q

T

T

I

M

L

K

R

A

A

F

F

G

G

A

A

E

E

L

L

I

V

L

L

T

T

P

D

K

P

S

A

A

R

G

G

M

M

E

K

Y

G

C

A

R

V

D

Q

L

K

A

A

D

E

R

E

M

I

Q

K

-

A

Q

K

E

T

G

P

K

N

G

S

R

Y

V

I

L

L

D

Q

Q

Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

R

K

I

I

H

H

Y

Y

E

E

T

T

G

G

P

P

E

E

I

I

W

W

T

R

D

G

T

S

F

G

G

G

K

K

I

I

T

D

H

A

F

L

V

V

S

S

A

G

M

I

G

G

T

T

T

G

G

G

T

T

I

V

T

T

G

G

V

A

S

G

R

K

Y

Y

L

L

K

K

E

E

Q

Q

N

N

P

P

A

N

I

I

Q

K

I

L

I

Y

G

G

A

V

Q

E

P

P

S

V

E

E

G

S

S

A

R

I

I

L

P

S

G

G

I

G

R

K

K

P

W

G

P

P

Q

H

E

K

-

I

-

Q

-

G

-

I

-

G

-

A

-

G

Y

F

L

I

P

P

K

G

I

V

Y

L

D

D

P

V

S

N

A

L

V

L

D

D

E

E

L

V

V

I

L

Q

V

V

S

S

Q

S

L

E

D

E

A

S

E

I

D

E

M

T

C

A

R

K

R

L

M

L

A

A

R

L

E

K

E

E

G

G

I

L

F

L

A

V

G

G

I

I

S

S

A

S

A

G

G

A

A

A

C

A

W

A

V

A

A

A

Q

I

E
x
R

I

I

A

A

K

K

T
x
R

V

P

Q

E

D

N

A

A

-

G

-

K

V

L

I

I

V

V

F

A

V

V

V

F

C

P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

V

V

L

F

F

binding phosphate ion: R277 (≠ E271), R281 (≠ T275)

3vbeC Crystal structure of beta-cyanoalanine synthase in soybean (see paper)
40% identity, 98% coverage: 4:298/300 of query aligns to 13:314/329 of 3vbeC

query
sites
3vbeC

Q

K

T

H

I

V

E

S

D

Q

A

L

V

I

G

G

K

R

T

T

P

P

L

L

V

V

A

Y

L

L

Q

N

R

K

I

V

A

T

A

E

A

G

D

C

N

-

A

-

R

-

G

G

N

A

I

Y

L

V

L

A

A

V

K

K

L

Q

E

E

G

M

N

M

N

Q

P

P

A

T

G

A

S

S

V

I

K
|
K

D

D

R

R

P

P

A

A

L

Y

S

A

M

M

I

I

R

T

R

D

A

A

E

E

R

K

G

N

D

L

I

I

K

T

P

P

G

G

D

K

T

T

-

T

L

L

I

I

E

E

A

P

T

T

S
|
S

G

G

N
|
N

T

M

G

G

I

I

A

S

L

M

A

A

M

F

A

M

A

A

I

M

K

K

G

G

Y

Y

R

K

M

M

I

V

L

L

I

T

M

M

P

P

E

S

D

Y

L

T

S

S

I

L

E

E

R

R

A

R

Q

V

T

T

M

M

K

R

A

A

F

F

G

G

A

A

E

E

L

L

I

I

L

L

T

T

P

D

K

P

S

A

A

K

G

G

M

M

E

G

-

G

Y

T

C

V

R

K

D

K

L

A

A

Y

D

E

R

L

M

L

Q

E

Q

N

E

T

G

P

K

N

G

A

R

H

V

M

L

L

D

Q

Q

Q

F

F

A

S

N

N

P

P

D

A

N

N

P

T

R

Q

I

V

H

H

Y

F

E

E

T

T

G

G

P

P

E

E

I

I

W

W

T

E

D

D

T

T

F

N

G

G

K

Q

I

V

T

D

H

I

F

F

V

V

S
x
M

A

G

M

I

G
|
G

T
x
S

T
x
G

G

G

T
|
T

I

V

T

S

G

G

V

V

S

G

R

Q

Y

Y

L

L

K

K

E

S

Q

K

N

N

P

P

A

N

I

V

Q

K

I

I

I

Y

G

G

A

V

Q
x
E

P

P

S

S

E

E

G
x
S

S

N

R

V

I

L

P

N

G

G

I

G

R

K

K

P

W

G

P

P

Q

H

E

H

-

I

-

T

-
x
G

-

N

-

G

-

V

-

G

Y

F

L

K

P

P

K

D

I

I

Y

L

D

D

P

L

S

D

A

V

V

M

D

E

E

K

L

V

V

L

L

E

V

V

S

S

Q

S

L

E

D

D

A

A

E

V

D

N

M

M

C

A

R

R

R

V

M

L

A

A

R

L

E

K

E

E

G

G

I

L

F

M

A

V

G

G

I

I

S
|
S

A

S

A

G

G

A

A

N

C

T

W

V

V

A

A

A

Q

L

E

R

I

L

A

A

K

Q

T

L

V

P

Q

E

D

N

-

K

-

G

A

K

V

L

I

I

V

V

F

T

V

V

V

H

C
x
P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

S

G

V

V

L

F

F

active site: K52 (= K47), S81 (= S75), E212 (≠ Q205), S216 (≠ G209), S275 (= S261), P302 (≠ C286)
binding pyridoxal-5'-phosphate: K52 (= K47), N83 (= N77), M184 (≠ S177), G187 (= G180), S188 (≠ T181), G189 (≠ T182), T191 (= T184), G231 (vs. gap), S275 (= S261), P302 (≠ C286)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
42% identity, 99% coverage: 3:298/300 of query aligns to 5:306/310 of 5xoqA

query
sites
5xoqA

F

F

Q

K

T

D

I

N

E

S

D

E

A

S

V

I

G

G

K

R

T

T

P

P

L

L

V

V

A

Q

L

I

Q

N

R

R

I

L

A

T

A

A

A

G

D

L

N

S

A

S

A

R

R

-

G

-

N

-

I

-

L

V

L

L

A

A

K

K

L

I

E

E

G

G

N

R

N

N

P

P

A

A

G

Y

S

S

V

V

K

K

D

C

R

R

P

I

A

G

L

A

S

A

M

M

I

I

R

W

R

D

A

A

E

E

E

Q

R

S

G

G

D

K

I

L

K

K

P

P

G

G

D

M

T

H

L

V

I

V

E

E

A

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

M

F

A

V

A

C

A

A

I

A

K

R

G

G

Y

Y

R

K

M

L

I

T

L

L

I

T

M

M

P

P

E

E

D

T

L

M

S

S

I

I

E

E

R

R

A

R

Q

M

T

M

M

L

K

K

A

S

F

F

G

G

A

A

E

D

L

L

I

V

L

L

T

T

P

P

K

G

S

A

A

D

G

G

M
|
M

E

K

Y

G

C

A

R

I

D

S

L

K

A

A

D

E

R

E

M

L

Q

A

E

Q

G

P

K

G

G

W

R

F

V

I

L

P

D

Q

Q
|
Q

F

F

A

K

N

N

P

P

D

A

N

N

P

P

R

A

I

I

H

H

Y

V

E

K

T

T

G

G

P

P

E

E

I

I

W

W

T

N

D

D

T

T

F

E

G

G

K

Q

I

V

T

D

H

V

F

F

V

V

S

A

A

G

M

V

G

G

T

T

T

G

G

G

T

T

I

I

T

T

G

G

V

V

S

A

R

R

Y

F

L

L

K

K

-

H

E

E

Q

K

N

K

P

H

A

P

I

V

Q

H

I

V

G

A

A

V

Q

E

P

P

S

A

E

A

G

S

S

P

R

V

I

L

P

A

G

G

-

G

-

P

-

A

-

G

-
x
R

-
x
H

-

K

I

I

R
x
Q

K
x
G

W

I

P

G

Q
x
A

E

G

Y

F

L

V

P

P

K

D

I

T

Y

F

D

D

P

R

S

S

A

V

V

V

D

D

E

E

L

I

V

L

L

S

V

V

S

T

Q

D

L

D

D

E

A

A

E

I

D

E

M

T

C

A

R

R

R

K

M

L

A

A

R

M

E

E

G

G

I

I

F

S

A

C

G

G

I

I

S

S

A

C

A

G

G

A

A

A

C

M

W

A

V

G

A

A

Q

L

E

K

I

V

A

A

K

A

T

R

V

P

Q

E

D

F

A

A

-

G

-

K

V

T

I

I

V

V

F

T

V

V

V

L

C

P

D

D

R

A

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

A

V

L

F

F

binding : T72 (= T74), S73 (= S75), G74 (= G76), T76 (= T78), M123 (= M125), Q144 (= Q146), R218 (vs. gap), H219 (vs. gap), Q222 (≠ R216), G223 (≠ K217), A226 (≠ Q220)

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
42% identity, 97% coverage: 4:293/300 of query aligns to 5:300/300 of 3zeiA

query
sites
3zeiA

Q

E

T

D

I

I

E

T

D

Q

A

L

V

I

G

G

K

R

T

T

P

P

L

L

V

V

A

R

L

L

Q

R

R

R

I

V

-

T

-

D

-

G

A

A

A

V

A

A

D

D

N

-

A

-

R

-

G

-

N

-

I

-

L

I

L

V

A

A

K

K

L

L

E

E

G

F

N

F

N

N

P

P

A

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

S

A

M

M

I

L

R

Q

R

A

A

A

E

E

E

Q

R

A

G

G

D

L

I

I

K

K

P

P

G

D

D

T

T

I

L

I

I

L

E

E

A

P

T
|
T

S
|
S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

M

A

V

A

C

A

A

I

A

K

R

G

G

Y

Y

R

R

M

C

I

V

L

L

I

T

M

M

P

P

E

E

D

T

L

M

S

S

I

L

E

E

R

R

A

R

Q

M

T

L

M

L

K

R

A

A

F

Y

G

G

A

A

E

E

L

L

I

I

L

L

T

T

P

P

K

G

S

A

A

D

G

G

M

M

E

S

Y

G

C

A

R
x
I

D

A

L

K

A

A

D

E

R

E

M

L

-

A

Q

K

Q

T

E

D

G

Q

K

R

G

Y

R

F

V

V

L

P

D

Q

Q
|
Q

F
|
F

A

E

N

N

P

P

D

A

N

N

P

P

R

A

I

I

H

H

Y

R

E

V

T

T

G

A

P

E

E

E

I

V

W

W

T

R

D

D

T

T

F

D

G

G

K

K

I

V

T

D

H

I

F

V

V

V

S

A

A

G

M
x
V

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

T

T

G

G

V

V

S

A

R

Q

Y

V

L

I

K

K

E

E

Q

R

N

K

P

P

A

S

I

A

Q

R

I

F

I

V

G

A

A

V

Q

E

P

P

S

A

E

A

G

S

S

P

R

V

I

L

P

S

G

G

I

G

R

Q

K
|
K

W

G

P

P

Q

H

E

P

-

I

-

Q

-
x
G

-

I

-

G

-
x
A

-

G

Y
x
F

L

V

P

P

K

P

I

V

Y

L

D

D

P

Q

S

D

A

L

V

V

D

D

E

E

L

I

V

I

L

T

V

V

S

G

Q

N

L

E

D

D

A

A

E

L

D

N

M

V

C

A

R

R

M

L

A

A

R

R

E

E

G

G

I

L

F

L

A

V

G

G

I

I

S
|
S

A

S

A

G

G

A

A

A

C

T

W

V

V

A

A

A

Q

L

E

Q

I

V

A

A

K

R

T

R

V

P

Q

E

D

N

A

A

-

G

-

K

V

L

I

I

V

V

F

V

V

V

V

L

C
x
P

D
|
D

R

F

G

G

D

E

R

R

Y

Y

L

L

active site: K44 (= K47), S266 (= S261), P293 (≠ C286)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T74), S72 (= S75), I126 (≠ R129), Q144 (= Q146), F145 (= F147), K215 (= K217), G222 (vs. gap), A225 (vs. gap), F227 (≠ Y222)
binding pyridoxal-5'-phosphate: K44 (= K47), N74 (= N77), V177 (≠ M179), G178 (= G180), T179 (= T181), G180 (≠ T182), T182 (= T184), G222 (vs. gap), S266 (= S261), P293 (≠ C286), D294 (= D287)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
42% identity, 97% coverage: 4:293/300 of query aligns to 5:300/300 of 2q3cA

query
sites
2q3cA

Q

E

T

D

I

I

E

T

D

Q

A

L

V

I

G

G

K

R

T

T

P

P

L

L

V

V

A

R

L

L

Q

R

R

R

I

V

-

T

-

D

-

G

A

A

A

V

A

A

D

D

N

-

A

-

R

-

G

-

N

-

I

-

L

I

L

V

A

A

K

K

L

L

E

E

G

F

N

F

N

N

P

P

A

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

S

A

M

M

I

L

R

Q

R

A

A

A

E

E

E

Q

R

A

G

G

D

L

I

I

K

K

P

P

G

D

D

T

T

I

L

I

I

L

E

E

A

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

M

M

A

V

A

C

A

A

I

A

K

R

G

G

Y

Y

R

R

M

C

I

V

L

L

I

T

M

M

P

P

E

E

D

T

L

M

S

S

I

L

E

E

R

R

A

R

Q

M

T

L

M

L

K

R

A

A

F

Y

G

G

A

A

E

E

L

L

I

I

L

L

T

T

P

P

K

G

S

A

A

D

G

G

M
|
M

E

S

Y

G

C

A

R

I

D

A

L

K

A

A

D

E

R

E

M

L

-

A

Q

K

Q

T

E

D

G

Q

K

R

G

Y

R

F

V

V

L

P

D

Q

Q
|
Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

R

A

I

I

H

H

Y

R

E

V

T

T

G

A

P

E

E

E

I

V

W

W

T

R

D

D

T

T

F

D

G

G

K

K

I

V

T

D

H

I

F

V

V

V

S

A

A

G

M

V

G

G

T

T

T

G

G

G

T

T

I

I

T

T

G

G

V

V

S

A

R

Q

Y

V

L

I

K

K

E

E

Q

R

N

K

P

P

A

S

I

A

Q

R

I

F

I

V

G

A

A

V

Q

E

P

P

S

A

E

A

G

S

S

P

R

V

I

L

P

S

G

G

I

G

R

Q

K
|
K

W

G

P

P

Q

H

E

P

-

I

-

Q

-
x
G

-

I

-

G

-
x
A

-

G

Y

F

L

V

P

P

K

P

I

V

Y

L

D

D

P

Q

S

D

A

L

V

V

D

D

E

E

L

I

V

I

L

T

V

V

S

G

Q

N

L

E

D

D

A

A

E

L

D

N

M

V

C

A

R

R

M

L

A

A

R

R

E

E

G

G

I

L

F

L

A

V

G

G

I

I

S
|
S

A

S

A

G

G

A

A

A

C

T

W

V

V

A

A

A

Q

L

E

Q

I

V

A

A

K

R

T

R

V

P

Q

E

D

N

A

A

-

G

-

K

V

L

I

I

V

V

F

V

V

V

V

L

C
x
P

D

D

R

F

G

G

D

E

R

R

Y

Y

L

L

active site: K44 (= K47), S266 (= S261), P293 (≠ C286)
binding : T71 (= T74), S72 (= S75), G73 (= G76), T75 (= T78), M122 (= M125), Q144 (= Q146), K215 (= K217), G222 (vs. gap), A225 (vs. gap)

3vc3A Crystal structure of beta-cyanoalanine synthase k95a mutant in soybean (see paper)
40% identity, 98% coverage: 6:298/300 of query aligns to 8:307/322 of 3vc3A

query
sites
3vc3A

I

V

E

S

D

Q

A

L

V

I

G

G

K

R

T

T

P

P

L

L

V

V

A

Y

L

L

Q

N

R

K

I

V

A

T

A

E

A

G

D

-

N

-

A

-

R

C

G

G

N

A

I

Y

L

V

L

A

A

V

K

K

L

Q

E

E

G

M

N

M

N

Q

P

P

A

T

G

A

S

S

V

I

K
x
A

D

D

R

R

P

P

A

A

L

Y

S

A

M

M

I

I

R

T

R

D

A

A

E

E

R

K

G

N

D

L

I

I

K

T

P

P

G

G

D

K

T

T

-

T

L

L

I

I

E

E

A

P

T
|
T

S
|
S

G

G

N
|
N

T
x
M

G

G

I

I

A

S

L

M

A

A

M

F

A

M

A

A

I

M

K

K

G

G

Y

Y

R

K

M

M

I

V

L

L

I

T

M

M

P

P

E

S

D

Y

L

T

S

S

I

L

E

E

R

R

A

R

Q

V

T

T

M

M

K

R

A

A

F

F

G

G

A

A

E

E

L

L

I

I

L

L

T

T

P

D

K

P

S

A

A

K

G

G

M

M

E

G

-

G

Y

T

C

V

R

K

D

K

L

A

A

Y

D

E

R

L

M

L

Q

E

Q

N

E

T

G

P

K

N

G

A

R

H

V

M

L

L

D

Q

Q
|
Q

F

F

A

S

N

N

P

P

D

A

N

N

P

T

R

Q

I

V

H

H

Y

F

E

E

T

T

G

G

P

P

E

E

I

I

W

W

T

E

D

D

T

T

F

N

G

G

K

Q

I

V

T

D

H

I

F

F

V

V

S
x
M

A

G

M

I

G
|
G

T
x
S

T
x
G

G

G

T
|
T

I

V

T

S

G

G

V

V

S

G

R

Q

Y

Y

L

L

K

K

E

S

Q

K

N

N

P

P

A

N

I

V

Q

K

I

I

I

Y

G

G

A

V

Q

E

P

P

S

S

E

E

G

S

S

N

R

V

I

L

P

N

G

G

I

G

R

K

K

P

W

G

P

P

Q

H

E

H

-

I

-

T

-
x
G

-

N

-

G

-

V

-

G

Y

F

L

K

P

P

K

D

I

I

Y

L

D

D

P

L

S

D

A

V

V

M

D

E

E

K

L

V

V

L

L

E

V

V

S

S

Q

S

L

E

D

D

A

A

E

V

D

N

M

M

C

A

R

R

R

V

M

L

A

A

R

L

E

K

E

E

G

G

I

L

F

M

A

V

G

G

I

I

S
|
S

A

S

A

G

G

A

A

N

C

T

W

V

V

A

A

A

Q

L

E

R

I

L

A

A

K

Q

T

L

V

P

Q

E

D

N

-

K

-

G

A

K

V

L

I

I

V

V

F

T

V

V

V

H

C
x
P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

S

G

V

V

L

F

F

active site: A45 (≠ K47), S268 (= S261), P295 (≠ C286)
binding n-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-cysteine: T73 (= T74), S74 (= S75), N76 (= N77), M77 (≠ T78), Q146 (= Q146), M177 (≠ S177), G180 (= G180), S181 (≠ T181), G182 (≠ T182), T184 (= T184), G224 (vs. gap), S268 (= S261), P295 (≠ C286)

Q9FS29 Bifunctional L-3-cyanoalanine synthase/cysteine synthase 2, mitochondrial; EC 2.5.1.47; EC 4.4.1.9 from Solanum tuberosum (Potato) (see paper)
39% identity, 98% coverage: 6:298/300 of query aligns to 32:331/347 of Q9FS29

query
sites
Q9FS29

I

V

E

S

D

Q

A

L

V

I

G

G

K

K

T

T

P

P

L

M

V

V

A

Y

L

L

Q

K

R

K

I

V

A

T

A

E

A

G

D

-

N

-

A

-

R

C

G

G

N

A

I

Y

L

I

L

A

A

V

K

K

L

Q

E

E

G

M

N

F

N

Q

P

P

A

T

G

S

S

S

V

I

K

K

D

D

R

R

P

P

A

A

L

L

S

A

M

M

I

I

R

N

R

D

A

A

E

E

E

K

R

K

G

G

D

L

I

I

K

S

P

P

-

E

G

K

D

T

T

T

L

L

I

I

E

E

A

P

T

T

S

S

G

G

N

N

T

M

G

G

I

I

A

S

L

M

A

A

M

F

A

M

A

A

I

M

K

K

G

G

Y

Y

R

K

M

M

I

V

L

L

I

T

M

M

P

P

E

S

D

Y

L

T

S

S

I

M

E

E

R

R

A

R

Q

V

T

T

M

M

K

R

A

A

F

F

G

G

A

A

E

D

L

L

I

I

L

L

T

T

P

D

K

P

S

T

A

K

G

G

M

M

E

G

-

G

Y

T

C

V

R

K

D

K

L

A

A

Y

D
x
E

R

L

M

L

Q

E

Q

S

E

T

G

P

K

N

G

A

R

F

V

M

L

L

D

Q

Q

Q

F

F

A

S

N

N

P

P

D

A

N

N

P

T

R

Q

I

V

H

H

Y

F

E

D

T

T

G

G

P

P

E

E

I

I

W

W

T

E

D

E

T

T

F

L

G

G

K

N

I

V

T

D

H

I

F

F

V

V

S

M

A

G

M

I

G

G

T

S

T

G

G

G

T

T

I

V

T

T

G

G

V

V

S

G

R

L

Y

Y

L

L

K

K

E

S

Q

K

N

N

P

P

A

N

I

V

Q

K

I

I

I

Y

G

G

A

L

Q

E

P

P

S

T

E

E

G

S

S

N

R

I

I

L

P

N

G

G

I

G

R

K

K

P

W

G

P

P

Q

H

E

H

Y

I

L

T

-

G

-

N

-

G

-

V

-

G

-

S

-

K

P

P

K

D

I

I

Y

V

D

D

P

M

S

D

A

L

V

M

D

E

E

E

L

V

V

L

L

M

V

V

S

S

Q

S

L

E

D

D

A

A

E

V

D

N

M

M

C

A

R

R

R

E

M

L

A

A

R

V

E

K

E

E

G

G

I

L

F

M

A

V

G

G

I

I

S

S

A

S

A

G

G

A

A

N

C

T

W

V

V

A

A

A

Q

L

E

R

I

L

A

A

K

Q

T

K

V

P

Q

E

D

N

-

K

-

G

A

K

V

L

I

I

V

V

F

T

V

V

V

H

C

A

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

S

G

V

V

L

F

Y

E157 (≠ D133) mutation E->N,Q: No effect on catalytic activities.

Query Sequence

>WP_011802541.1 NCBI__GCF_000015505.1:WP_011802541.1
MKFQTIEDAVGKTPLVALQRIAAADNAARGNILLAKLEGNNPAGSVKDRPALSMIRRAEE
RGDIKPGDTLIEATSGNTGIALAMAAAIKGYRMILIMPEDLSIERAQTMKAFGAELILTP
KSAGMEYCRDLADRMQQEGKGRVLDQFANPDNPRIHYETTGPEIWTDTFGKITHFVSAMG
TTGTITGVSRYLKEQNPAIQIIGAQPSEGSRIPGIRKWPQEYLPKIYDPSAVDELVLVSQ
LDAEDMCRRMAREEGIFAGISAAGACWVAQEIAKTVQDAVIVFVVCDRGDRYLSTGVFPA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory