SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011803058.1 NCBI__GCF_000015505.1:WP_011803058.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

5uhsA Structure of a semisweet d57a mutant (see paper)
48% identity, 92% coverage: 3:81/86 of query aligns to 1:79/81 of 5uhsA

query
sites
5uhsA

L

M

T

E

D

N

L

L

I

I

G

G

Y

Y

C

V

A

A

A

A

T

F

L

L

T

T

T

T

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S

F

F

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P

P

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Q

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T

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D

Q

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R

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S

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N

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M

Y

Y

S

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F
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F

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F

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L

G

G

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W
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W

T

F

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V

Y

Y

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G

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L

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A

A

W

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P

P

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A

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F

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A

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Y

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K

L

L

binding beta-D-glucopyranose: F41 (= F43), W48 (= W50)

B0SR19 Sugar transporter SemiSWEET from Leptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris) (see paper)
47% identity, 92% coverage: 3:81/86 of query aligns to 1:79/85 of B0SR19

query
sites
B0SR19

L

M

T

E

D

N

L

L

I

I

G

G

Y

Y

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A

A

A

A

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F

L

L

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T

T

T

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S

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F

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L

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P

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Q

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L

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A

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N

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F

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K

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L

W48 (= W50) mutation to A: Impairs glucose transport.
N64 (= N66) mutation to A: Impairs glucose transport.

P0DMV3 Sugar transporter SemiSWEET from Escherichia coli (strain UMEA 3162-1) (see paper)
41% identity, 71% coverage: 21:81/86 of query aligns to 21:81/89 of P0DMV3

query
sites
P0DMV3

P
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P

Q
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Q

A

S

W

I

H

K

T

T

F

I

T

R

T

T

R

R

D

N

V

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R

E

G

G

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M

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Y

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F

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W

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Y

G

G

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L

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M
x
R

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D

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F

P

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V

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A

N
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N

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F

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P21 (= P21) mutation to A: Strongly decreases sucrose transporter activity.
Q22 (= Q22) mutation to A: Slightly decreases sucrose transporter activity.
M39 (= M39) mutation to A: No effect on sucrose transporter activity.
Y40 (= Y40) mutation to A: Decreases sucrose transporter activity.
W50 (= W50) mutation to A: Increases sucrose transporter activity.; mutation to F: No effect on sucrose transporter activity.
Y53 (= Y53) mutation to F: Increases sucrose transporter activity.
R57 (≠ M57) mutation to A: Increases sucrose transporter activity.
N66 (= N66) mutation to A: Impairs sucrose transporter activity.

Sites not aligning to the query:

19 F→A: Decreases sucrose transporter activity.

Query Sequence

>WP_011803058.1 NCBI__GCF_000015505.1:WP_011803058.1
MLLTDLIGYCAATLTTASFVPQAWHTFTTRDVRGISLGMYSAFTVGVMCWTVYGLLMDAW
PIVVANCITLVLALAILVMKLRYHQA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory