SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011808619.1 NCBI__GCF_000015565.1:WP_011808619.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P78061 Gamma-glutamylputrescine synthetase PuuA; Gamma-Glu-Put synthetase; Glutamate--putrescine ligase; EC 6.3.1.11 from Escherichia coli (strain K12) (see paper)
34% identity, 97% coverage: 13:453/456 of query aligns to 31:469/472 of P78061

query
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P78061

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H282 (= H266) mutation to N: Activity is impaired to 9% of wild-type.
R357 (= R342) mutation to Q: Activity is impaired to 3% of wild-type.

8s59O Glutamine synthetase
33% identity, 90% coverage: 43:453/456 of query aligns to 25:443/446 of 8s59O

query
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8s59O

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binding 2-oxoglutaric acid: R25 (= R43), A39 (= A57), R86 (vs. gap), V92 (≠ T103), Q168 (≠ I180), R171 (≠ V183), R172 (≠ N184), S188 (≠ L207)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 23

8tfbA Glutamine synthetase (see paper)
33% identity, 90% coverage: 43:453/456 of query aligns to 22:440/443 of 8tfbA

query
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8tfbA

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V

V

F

T

S

V

H

Y

Y

S

L

E

C

I

A

K

K

E

E

L

M

E

E

F

W

D

D

E

E

F

Y

M

K

K

A

V

V

I

V

S

H

P

P

W

W

E

E

R

L

E

S

H

R

L

Y

L

L

binding magnesium ion: E133 (= E147), E188 (= E210), E195 (= E217)

8tfkA Glutamine synthetase (see paper)
33% identity, 90% coverage: 43:453/456 of query aligns to 19:437/440 of 8tfkA

query
sites
8tfkA

R

R

A

T

K

Q

F

F

T

T

E

D

D

T

R

L

G

G

M

I

R

I

L

K

P

S

Q

W

A

A

I

I

V

P

A

A

M

E

G

Q

V

L

T

E

G

E

E

A

F

F

P

-

Q

E

S

N

G

G

P

V

Y

M

Y

F

D

D

-

G

-

S

-

I

-

Q

-

G

V

F

I

T

D

R

P

I

T

E

D

E

K

S

D

D

M

M

Q

K

L

L

R

A

P

L

D

D

P

P

S

S

S

T

V

F

R

R

I

I

V

L

P

P

W

W

-

R

-

P

A

A

T

T

D

G

P

A

T

V

A

A

Q

R

V

I

I

L

H

G

D

D

C

V

F

Y

D

L

N

P

E

D

G

G

N

N

M

P

V

F

P

K

F

G

A

D

P

P

R

R

S

Y

V

V

L

L

R

K

R

T

V

A

L

I

G

K

L

E

F

A

A

E

A

K

Q

M

G

G

L

F

Q

S

P

M

I

N

V

V

A

G

P

P

E
|
E

L

L

E
|
E

F

F

Y

F

L

L

T

F

A

-

R

-

N

K

T

L

D

D

P

A

N

N

M

G

L

N

P

P

R

T

P

T

P

E

I

L

-

T

G

D

R

Q

S

G

G

G

R

Y

A

F

E

D

T

F

S

A

R

P

Q

L

A

D

Y

R

S

A

I

Q

D

D

A

V

V

R

N

R

E

D

F

I

D

D

P

Y

L

A

F

L

E

E

E

H

I

-

Y

-

D

-

F

-

S

-

D

-

K

-

M

M

E

G

L

F

N

Q

V

I

D

E

T

A

L

S

I

H

H

H

E
|
E

V

V

G

A

A

P

G

S

Q

Q

M

H

E
|
E

I

I

N
x
D

F
|
F

F

R

H
x
F

N

G

V

D

P

V

L

L

G

C

L

T

A

A

D

D

E

N

V

V

F

V

F

T

F

F

K

K

R

Y

T

V

V

V

R

K

E

S

S

I

A

A

L

Y

R

H

H

K

D

G

M

Y

Y

Y

A

A

T

S

F

F

M

M

A

P

K

K

P

P

I

L

S

F

G

G

E

V

P

N

G
|
G

S

S

A

G

M

M

H
|
H

V

S

H
x
N

Q

Q

S

S

L

L

L

F

D

-

V

-

A

K

S

D

G

G

K

K

N

N

V

V

F

F

S

Y

N

D

E

P

D

D

G

-

T

T

P

P

S

T

A

K

M

L

-

S

-

Q

-

D

F

A

M

M

H

Y

Y

Y

I

I

G

G

L

L

Q

L

R

K

Y

H

I

I

P

R

A

E

A

F

M

T

V

A

L

V

V

T

A

N

P

P

Y

V

V

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

S

V

R

P

N

G

T

Y

A
x
E

A

A

P

P

I

V

N

Y

I

I

E

S

W

W

G

S

Y

A

D

Q

N

N

R
|
R

T

S

V

S

G

L

I

I

R
|
R

S

I

P

P

I

A

S

T

T

R

P

G

A

N

A
x
G

R

T

R
|
R

V

I

E
|
E

N

L

R
|
R

V

C

I

P

G

D

A

P

D

A

A

C

N

N

P

P

Y

Y

V

L

A

A

M

F

A

A

M

L

T

M

L

L

A

R

C

A

G

G

Y

L

L

E

G

G

I

I

M

K

N

N

K

K

I

I

E

D

P

P

K

G

A

E

E

P

M

T

K

N

G

V

D

N

A

I

Y

F

L

H

C

L

A

S

-

D

-

K

-

E

-

R

-

E

-

R

-

G

-

I

Y

R

A

S

L

L

P

P

R

A

S

D

L

L

A

K

E

E

A

A

L

I

D

D

W

E

L

M

R

K

K

G

E

S

T

K

D

F

L

V

H

K

E

E

V

A

L

L

G

G

K

E

E

H

F

V

V

F

T

S

V

H

Y

Y

S

L

E

C

I

A

K

K

E

E

L

M

E

E

F

W

D

D

E

E

F

Y

M

K

K

A

V

V

I

V

S

H

P

P

W

W

E

E

R

L

E

S

H

R

L

Y

L

L

binding adenosine-5'-diphosphate: E128 (= E145), D194 (≠ N219), F195 (= F220), F197 (≠ H222), N243 (≠ H268), R312 (= R337), R317 (= R342), G325 (≠ A350), R327 (= R352)
binding magnesium ion: E128 (= E145), E128 (= E145), E130 (= E147), E185 (= E210), E192 (= E217), E192 (= E217), H241 (= H266), E329 (= E354)
binding l-methionine-s-sulfoximine phosphate: E128 (= E145), E130 (= E147), E185 (= E210), E192 (= E217), G237 (= G262), H241 (= H266), R294 (= R319), E300 (≠ A325), R312 (= R337), R331 (= R356)

8oozA Glutamine synthetase (see paper)
32% identity, 84% coverage: 71:453/456 of query aligns to 46:427/430 of 8oozA

query
sites
8oozA

G

G

P

I

Y

H

Y

F

D

D

V

G

I

S

D

S

P

I

T

E

D

G

K

S

D

D

M

M

Q

V

L

L

R

R

P

P

D

D

P

P

S

D

S

T

V

F

R

R

I

V

V

L

P

P

W

W

A

S

T

T

D

-

P

A

T

E

A

A

Q

R

V

L

I

I

H

C

D

D

C

I

F

E

D

L

N

P

E

D

G

G

N

K

M

P

V

F

P

M

F

G

A

C

P

P

R

R

S

Q

V

V

L

L

R

K

V

N

L

M

G

E

L

E

F

A

A

A

A

K

Q

L

G

G

L

Y

Q

V

P

M

I

N

V

T

A
x
G

P

P

E
|
E

L

M

E

E

F

F

Y

F

L

L

T

F

A

K

R

R

N

Q

T

D

D

-

P

-

N

G

M

M

L

P

P

T

R

N

P

I

P

P

I

Q

G

D

R

R

S

G

G

G

R

Y

A

F

E

D

T

L

S

A

R

-

Q

-

A

-

Y

-

S

P

I

I

D

D

A

L

V

A

N

E

E

E

F

I

D

K

P

-

L

-

F

-

E

R

E

E

I

I

Y

V

D

L

F

V

S

L

D

E

K

E

M

M

E

G

L

F

N

E

V

V

D
x
E

T

A

L

A

I

H

H

H

E

E

V

V

G

A

A

F

G

G

Q

Q

M

H

E

E

I

I

N

D

F
|
F

F
x
K

H
x
Y

N

D

V

N

P

A

L

L

G

A

L

T

A

A

D

D

E

N

V

V

F

I

F

T

F

L

K

K

R

Y

T

V

V

A

R

K

E

T

S

L

A

A

L

L

R

Q

H

H

D

G

M

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

I

S

F

G

G

E

V

P

N

G

G

S

S

A

G

M

M

H
|
H

V

T

H
x
N

Q

T

S
|
S

L

L

L

F

D

-

V

-

A

K

S

D

G

G

K

K

N

N

V

A

F

F

S

Y

N

D

E

P

D

D

G

A

-

P

-

D

T

Q

P

I

S

S

A

D

M

T

F

L

M

R

H

Y

Y

F

I

V

G

G

L

V

Q

L

R

K

Y

H

I

I

P

R

A

A

A

I

M

T

V

A

L

I

V

T

A

N

P

P

Y

L

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

S

V

R

P

N

G

T

Y

A

E

A

A

P

P

I

V

N

Y

I

I

E

T

W

W

G

S

Y

G

D

P

N

N

R

R

T

S

V

S

G

L

I

I

R

R

S

V

P

P

I

A

S

P

T

R

P

G

A

N

A
x
S

R

T

R
|
R

V

I

E
|
E

N

I

R

R

V

S

I

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

V

L

A

A

M

F

A

A

M

A

T

I

L

L

A

A

C

A

G

G

Y

L

L

D

G

G

I

V

M

K

N

N

K

K

I

I

E

E

P

P

K

P

A

E

E

R

M

V

K

E

G

K

D

N

A

I

Y

Y

-

K

-

L

-

T

-

E

-

R

-

E

-

K

L

L

C

G

A

I

Y

G

A

M

L

L

P

P

R

G

S

T

L

L

A

K

E

E

A

A

L

I

D

E

W

C

L

F

R

K

K

E

E

D

T

E

D

L

L

L

H

V

E

S

V

A

L

L

G

G

K

E

E

H

F

V

V

S

T

Q

V

S

Y

I

S

I

E

N

I

V

K

A

E

M

L

A

E

D

F

W

D

D

E

S

F

Y

M

R

K

T

V

Q

I

V

S

H

P

Q

W

W

E

E

R

L

E

D

H

R

L

Y

L

L

binding adenosine-5'-triphosphate: G117 (≠ A143), E170 (≠ D205), F185 (= F220), K186 (≠ F221), Y187 (≠ H222), N233 (≠ H268), S235 (= S270), S315 (≠ A350), R317 (= R352)
binding magnesium ion: E119 (= E145), H231 (= H266), E319 (= E354)

5dm3C Crystal structure of glutamine synthetase from chromohalobacter salexigens dsm 3043(csal_0679, target efi-550015) with bound adp
33% identity, 80% coverage: 86:448/456 of query aligns to 56:390/396 of 5dm3C

query
sites
5dm3C

L

M

R

K

P

P

D

D

P

L

S

A

S

T

V

L

R

R

I

C

V

V

P

P

W

W

A

-

T

L

D

E

P

G

T

T

A

A

Q

M

V

V

I

L

H

C

D

D

C

L

F

L

D

D

N

H

E

R

G

T

N

H

M

A

-

E

V

V

P

P

F

H

A

A

P

P

R

R

S

A

V

I

L

L

R

K

R

R

V

Q

L

L

G

A

L

R

F

L

A

E

A

A

Q

M

G

G

L

L

Q

E

P

A

I

I

V

M

A

A

P

T

E
|
E

L

L

E
|
E

F

F

Y

F

L

L

T

F

A

E

R

K

N

S

T

L

D

D

P

E

N

I

M

-

L

-

P

-

R

-

P

-

I

-

G

-

R

R

S

K

G

G

R

R

A

F

E

R

T

T

S

T

R

K

Q

E

A

E

Y

H

S

V

I

L

D

R

A

-

V

-

N

-

E

-

F

-

D

-

P

P

L

L

F

R

E

N

E

H

I

L

Y

H

D

A

F

A

S

G

D

-

K

-

M

-

E

-

L

I

N

P

V

V

D

E

T

G

L

T

I

K

H

G

E
|
E

V

A

G

G

A

A

G

G

Q

Q

M

E

E
|
E

I

L

N

N

F

I

F
x
R

H
x
C

N

A

V

K

P

A

L

L

G

D

L

T

A

A

D

D

E

Y

V

H

F

T

F

I

F

A

K

K

R

H

T

A

V

T

R

K

E

E

S

I

A

A

L

W

R

Q

H

Q

D

G

M

R

Y

A

A

V

T

T

F

F

M

L

A

S

K

K

P

W

I

H

S

H

G

A

E

H

P

A

G

G

S

S

A

S

M

S

H
|
H

V

I

H
|
H

Q

Q

S
|
S

L

L

L

W

D

K

V

-

A

-

S

Q

G

G

K

L

N

P

V

A

F

F

S

H

N

D

E

E

D

R

G

D

T

A

-

L

-

G

P

M

S

S

A

A

M

L

F

M

M

K

H

H

Y

Y

I

L

G

A

G

G

L

L

Q

L

R

K

Y

Y

I

A

P

P

A

D

A

Y

M

T

V

Y

L

F

V

L

A

A

P

P

Y

Y

V

L

N

N

S

S

Y

Y

R

K

R

R

L

F

S

Q

R

-

N

-

T

-

A

F

A

A

P

P

I

T

N

R

I

T

E

V

W

W

G

S

Y

V

D

D

N

N

R
|
R

T

T

V

A

G

G

I

F

R

R

S

L

P

C

I

A

S

E

T

G

P

T

A

R

A

A

R

V

R
|
R

V

I

E
|
E

N

C

R
|
R

V

I

I

G

G

G

A

S

D

D

A

L

N

N

P

P

Y

Y

V

L

A

A

M

M

A

A

M

G

T

Q

L

L

A

A

C

A

G

G

Y

I

L

K

G

G

I

I

M

E

N

E

K

C

I

L

E

A

P

L

K

P

A

P

E

P

M

A

K

S

G

G

D

-

A

-

Y

-

L

-

C

-

A

-

Y

-

A

L

L

I

P

P

R

Q

S

N

L

L

A

R

E

D

A

A

L

M

D

E

W

A

L

L

R

R

K

G

E

S

T

T

D

M

L

L

H

R

E

E

V

A

L

M

G

G

K

E

E

D

F

V

V

V

T

D

V

H

Y

Y

S

V

E

R

I

A

K

A

E

E

L

V

E

E

F

L

D

E

E

D

F

F

M

Q

K

R

V

V

I

V

S

S

P

D

W

Y

E

E

active site: E115 (= E145), E117 (= E147), E162 (= E210), E169 (= E217), H218 (= H266), R286 (= R337), E303 (= E354), R305 (= R356)
binding adenosine-5'-diphosphate: R173 (≠ F221), C174 (≠ H222), H220 (= H268), S222 (= S270), R301 (= R352)

8ooqB Glutamine synthetase from Methanothermococcus thermolithotrophicus (see paper)
31% identity, 83% coverage: 69:448/456 of query aligns to 50:439/446 of 8ooqB

query
sites
8ooqB

Q

E

S

N

G

G

P

L

Y

Y

Y

F

D

D

-

G

-

S

-

I

-

E

-

G

V

F

I

V

D

G

P

I

T

N

D

E

K

S

D

D

M

M

Q

M

L

L

R

K

P

P

D

D

P

L

S

S

S

T

V

F

R

S

I

V

V

L

P

P

W

W

-
x
R

-

P

A

S

T

E

D

K

P

S

T
x
V

A

A

Q

R

V

V

I

I

H

C

D

D

C

V

F

Y

D

T

N

T

E

K

G

G

N

K

M

P

V

F

P

E

F

G

A

D

P

P

R

R

S

G

V

C

L

L

R

K

R

R

V

V

L

M

G

E

L

E

F

F

A

K

Q

E

-

F

G

N

L

G

Q

E

P

Y

I

F

V

V

A

G

P

P

E

E

L

P

E
|
E

F

F

Y

F

L

L

T

L

A

K

R

K

N

-

T

-

D

D

P

P

-

H

N

N

M

P

L

H

P

K

R

Y

P

I

P

P

I

A

G

D

R

D

S

G

G

G

R

Y

A

-

E

-

T

-

S

-

R

-

Q

-

A

-

Y

F

S

D

I

L

D

E

A

P

V

M

N

D

E

E

F

A

D
x
P

P

D

L

I

F
x
R

E
x
R

E

D

I

I

Y

V

D

F

F

A

S

L

D

E

K

N

M

L

E

G

L

F

N

H

V

V

D

E

T

A

L
x
S

I

H

H

H

E
|
E

V

V

G

A

A

P

G

G

Q

Q

M

H

E
|
E

I

V

N

D

F

F

F

K

H

F

N

D

V

D

P

A

L

L

G

K

L

T

A

A

D

D

E

S

V

V

F

I

F

T

F

F

K

K

R

T

T

T

V

I

R

K

E

T

S

I

A

A

L

E

R

Q

H

H

D

G

M

L

Y

K

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

F

S

F

G

G

E

M

P

N

G

G

S

S

A

G

M

M

H

H

V

C

H

H

Q

Q

S

S

L

I

L

W

D

-

V

-

A

L

S

N

G

G

K

E

N

P

V

S

F

F

S

Y

N

D

E

E

D

N

G

A

-

P

-

Y

T

Q

P

L

S

S

A

E

M

T

F

C

M

M

H

N

Y

Y

I

V

G

A

G

G

L

I

Q

L

R

K

Y

H

I

A

P

K

A

A

A

I

M

V

V

A

L

I

V

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

S

V

R

P

N

G

T

Y

A

E

A

A

P

P

I

V

N

N

I

I

E

A

W

W

G

A

Y

N

D

S

N

N

R

R

T

S

V

A

G

I

I

I

R

R

S

V

P

P

I

A

S

A

T

R

P

G

A

K

A

G

R

T

R

R

V

I

E

E

N

F

R

R

V

A

I

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

V

L

A

A

M

F

A

T

M

V

T

M

L

L

A

A

C

A

G

G

Y

L

L

D

G

G

I

V

M

K

N

N

K

K

I

L

E

D

P

A

K

P

A

E

E

P

M

V

K

E

G

R

D

N

A

I

Y

F

L

A

C

M

A

S

Y

E

A

A

-

E

-

K

-

E

-

L

-

G

-

I

-

E

-

S

L

V

P

P

R

A

S

N

L

L

A

K

E

A

A

A

L

L

D

D

W

E

L

L

R

E

K

N

E

N

T

D

D

V

L

L

H

K

E

N

V

A

L

L

G

G

K

K

E

H

F

I

V

F

T

E

V

S

Y

F

S

L

E

E

I

I

K

K

E

N

L

A

E

E

F

W

D

D

E

S

F

F

M

R

K

T

V

S

I

V

S

T

P

D

W

W

E

E

binding 2-oxoglutaric acid: R86 (vs. gap), V92 (≠ T103), P169 (≠ D187), R172 (≠ F190), R173 (≠ E191), S189 (≠ L207)
binding magnesium ion: E137 (= E147), E192 (= E210), E199 (= E217)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 16, 18, 32

8oooA Glutamine synthetase from methanothermococcus thermolithotrophicus in complex with 2-oxoglutarate and mgatp at 2.15 a resolution (see paper)
31% identity, 83% coverage: 69:448/456 of query aligns to 51:440/447 of 8oooA

query
sites
8oooA

Q

E

S

N

G

G

P

L

Y

Y

Y

F

D

D

-

G

-

S

-

I

-

E

-

G

V

F

I

V

D

G

P

I

T

N

D

E

K

S

D

D

M

M

Q

M

L

L

R

K

P

P

D

D

P

L

S

S

S

T

V

F

R

S

I

V

V

L

P

P

W

W

-
x
R

-

P

A

S

T

E

D

K

P

S

T
x
V

A

A

Q

R

V

V

I

I

H

C

D

D

C

V

F

Y

D

T

N

T

E

K

G

G

N

K

M

P

V

F

P

E

F

G

A

D

P

P

R

R

S

G

V

C

L

L

R

K

R

R

V

V

L

M

G

E

L

E

F

F

A

K

Q

E

-

F

G

N

L

G

Q

E

P

Y

I

F

V

V

A

G

P

P

E
|
E

L

P

E

E

F

F

Y

F

L

L

T

L

A

K

R

K

N

-

T

-

D

D

P

P

-

H

N

N

M

P

L

H

P

K

R

Y

P

I

P

P

I

A

G

D

R

D

S

G

G

G

R

Y

A

-

E

-

T

-

S

-

R

-

Q

-

A

-

Y

F

S

D

I

L

D

E

A

P

V

M

N

D

E

E

F

A

D
x
P

P

D

L

I

F
x
R

E
x
R

E

D

I

I

Y

V

D

F

F

A

S

L

D

E

K

N

M

L

E

G

L

F

N

H

V

V

D
x
E

T

A

L
x
S

I

H

H

H

E

E

V

V

G

A

A

P

G

G

Q

Q

M

H

E

E

I

V

N

D

F
|
F

F
x
K

H
x
F

N

D

V

D

P

A

L

L

G

K

L

T

A

A

D

D

E

S

V

V

F

I

F

T

F

F

K

K

R

T

T

T

V

I

R

K

E

T

S

I

A

A

L

E

R

Q

H

H

D

G

M

L

Y

K

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

F

S

F

G

G

E

M

P

N

G

G

S

S

A

G

M

M

H

H

V

C

H
|
H

Q

Q

S
|
S

L

I

L

W

D

-

V

-

A

L

S

N

G

G

K

E

N

P

V

S

F

F

S

Y

N

D

E

E

D

N

G

A

-

P

-

Y

T

Q

P

L

S

S

A

E

M

T

F

C

M

M

H

N

Y

Y

I

V

G

A

G

G

L

I

Q

L

R

K

Y

H

I

A

P

K

A

A

A

I

M

V

V

A

L

I

V

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

S

V

R

P

N

G

T

Y

A

E

A

A

P

P

I

V

N

N

I

I

E

A

W

W

G

A

Y

N

D

S

N

N

R

R

T

S

V

A

G

I

I

I

R
|
R

S

V

P

P

I

A

S

A

T

R

P

G

A

K

A

G

R

T

R
|
R

V

I

E

E

N

F

R

R

V

A

I

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

V

L

A

A

M

F

A

T

M

V

T

M

L

L

A

A

C

A

G

G

Y

L

L

D

G

G

I

V

M

K

N

N

K

K

I

L

E

D

P

A

K

P

A

E

E

P

M

V

K

E

G

R

D

N

A

I

Y

F

L

A

C

M

A

S

Y

E

A

A

-

E

-

K

-

E

-

L

-

G

-

I

-

E

-

S

L

V

P

P

R

A

S

N

L

L

A

K

E

A

A

A

L

L

D

D

W

E

L

L

R

E

K

N

E

N

T

D

D

V

L

L

H

K

E

N

V

A

L

L

G

G

K

K

E

H

F

I

V

F

T

E

V

S

Y

F

S

L

E

E

I

I

K

K

E

N

L

A

E

E

F

W

D

D

E

S

F

F

M

R

K

T

V

S

I

V

S

T

P

D

W

W

E

E

binding 2-oxoglutaric acid: R87 (vs. gap), V93 (≠ T103), P170 (≠ D187), R173 (≠ F190), R174 (≠ E191), S190 (≠ L207)
binding adenosine-5'-triphosphate: E136 (= E145), E188 (≠ D205), F203 (= F220), K204 (≠ F221), F205 (≠ H222), H251 (= H268), S253 (= S270), R325 (= R342), R335 (= R352)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 17, 19, 33

8ooxB Glutamine synthetase (see paper)
32% identity, 82% coverage: 81:453/456 of query aligns to 62:435/438 of 8ooxB

query
sites
8ooxB

D

E

K

S

D

D

M

M

Q

V

L

L

R

R

P

P

D

D

P

P

S

D

S

T

V

F

R

R

I

V

V

L

P

P

W

W

A

S

T

G

D

N

P

T

T

A

-

E

A

A

Q

R

V

L

I

I

H

C

D

D

C

I

F

E

D

L

N

P

E

D

G

G

N

K

M

P

V

F

P

M

F

G

A

C

P

P

R

R

S

Q

V

V

L

L

R

K

V

N

L

M

G

E

L

E

F

A

A

A

A

K

Q

L

G

G

L

Y

Q

V

P

M

I

N

V

T

A

G

P

P

E

E

L

M

E
|
E

F

F

Y

F

L

L

T

F

A

K

R

R

N

Q

T

D

D

-

P

-

N

G

M

M

L

P

P

T

R

N

P

I

P

P

I

Q

G

D

R

R

S

G

G

G

R

Y

A

F

E

D

T

L

S

A

R

-

Q

-

A

-

Y

-

S

P

I

I

D

D

A

L

V

A

N

E

E

E

F

I

D

K

P

-

L

-

F

-

E

R

E

E

I

I

Y

V

D

L

F

V

S

L

D

E

K

E

M

M

E

G

L

F

N

E

V

V

D

E

T

A

L

A

I

H

H

H

E
|
E

V

V

G

A

A

F

G

G

Q

Q

M

H

E
|
E

I

I

N

D

F

F

F

K

H

Y

N

D

V

N

P

A

L

L

G

A

L

T

A

A

D

D

E

N

V

V

F

I

F

T

F

L

K

K

R

Y

T

V

V

A

R

K

E

T

S

L

A

A

L

L

R

Q

H

H

D

G

M

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

I

S

F

G

G

E

V

P

N

G

G

S

S

A

G

M

M

H

H

V

T

H

N

Q

T

S

S

L

L

L

F

D

-

V

-

A

K

S

D

G

G

K

K

N

N

V

A

F

F

S

Y

N

D

E

P

D

D

G

A

-

P

-

D

T

Q

P

I

S

S

A

D

M

T

F

L

M

R

H

Y

Y

F

I

V

G

G

L

V

Q

L

R

K

Y

H

I

I

P

R

A

A

A

I

M

T

V

A

L

I

V

T

A

N

P

P

Y

L

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

S

V

R

P

N

G

T

Y

A

E

A

A

P

P

I

V

N

Y

I

I

E

T

W

W

G

S

Y

G

D

P

N

N

R

R

T

S

V

S

G

L

I

I

R

R

S

V

P

P

I

A

S

P

T

R

P

G

A

N

A

S

R

T

R

R

V

I

E

E

N

I

R

R

V

S

I

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

V

L

A

A

M

F

A

A

M

A

T

I

L

L

A

A

C

A

G

G

Y

L

L

D

G

G

I

V

M

K

N

N

K

K

I

I

E

E

P

P

K

P

A

E

E

R

M

V

K

E

G

K

D

N

A

I

Y

Y

-

K

-

L

-

T

-

E

-

R

-

E

-

K

L

L

C

G

A

I

Y

G

A

M

L

L

P

P

R

G

S

T

L

L

A

K

E

E

A

A

L

I

D

E

W

C

L

F

R

K

K

E

E

D

T

E

D

L

L

L

H

V

E

S

V

A

L

L

G

G

K

E

E

H

F

V

V

S

T

Q

V

S

Y

I

S

I

E

N

I

V

K

A

E

M

L

A

E

D

F

W

D

D

E

S

F

Y

M

R

K

T

V

Q

I

V

S

H

P

Q

W

W

E

E

R

L

E

D

H

R

L

Y

L

L

binding magnesium ion: E129 (= E147), E183 (= E210), E190 (= E217)

4s0rD Structure of gs-tnra complex (see paper)
29% identity, 97% coverage: 12:453/456 of query aligns to 11:444/447 of 4s0rD

query
sites
4s0rD

D

D

L

I

E

E

Q

K

W

L

L

V

N

K

E

E

R

E

H

N

V

V

T

K

E

Y

V

I

E

R

C

L

L

Q

V

F

P

T

D

D

L

I

T

L

G

G

V

T

A

I

R

K

G

N

K

V

I

E

L

I

P

P

R

V

A

S

K

Q

F

L

T

-

E

-

D

-

R

-

G

G

M

K

R

A

L

L

P

D

Q

N

A

K

I

V

V

M

A

F

M
x
D

G

G

V

S

T

S

G

I

E

E

F

-

P

-

Q

-

S

-

G

-

P

-

Y

-

D

G

V
x
F

I
x
V

D
x
R

P
x
I

T

E

D

E

K

S

D

D

M

M

Q

Y

L

L

R

Y

P

P

D

D

P

L

S

N

S

T

V

F

R

V

I

I

V

F

P

P

W

W

A

T

T

A

D

E

P

K

-

G

-

K

T

V

A

A

Q

R

V

F

I

I

H

C

D

D

C

I

F

Y

D

N

N

P

E

D

G

G

N

T

M

P

V

F

P

E

F

G

A

D

P

P

R

R

S

N

V

N

L

L

R

K

R

R

V

I

L

L

G

K

L

E

F

M

A

E

A

D

Q

L

G

G

L

F

Q

S

P

D

I

F

-

N

V

L

A

G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

T

F

A

-

R

-

N

K

T

L

D

D

P

E

N

K

M

G

L

E

P

P

R

T

P

L

P

E

I

L

G

N

R

D

S

K

G

G

R

-

A

-

E

-

T

-

S

-

R

-

Q

G

A

Y

Y

F

S
x
D

I

L

D

A

A

P

V

T

N

D

E

L

F

G

D

E

P

N

L

C

F

R

E

R

E

D

I

I

Y

V

D

L

F

E

S

L

D

E

K

E

M

M

E

G

L

F

N

E

V

I

D

E

T

A

L

S

I

H

H

H

E
|
E

V

V

G

A

A

P

G

G

Q

Q

M

H

E
|
E

I

I

N

D

F

F

F

K

H

Y

N

A

V

G

P

A

L

V

G

R

L

S

A

C

D

D

E

D

V

I

F

Q

F

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

S

I

A

A

L

R

R

K

H

H

D

G

M

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

S

F

G
|
G

E
x
V

P
x
N

G

G

S

S

A

G

M

M

H
|
H

V

C

H

N

Q

L

S

S

L

L

L

F

D

-

V

-

A

K

S

N

G

G

K

V

N

N

V

A

F

F

-

F

-

D

S

E

N

N

E

A

D

D

G

L

T

Q

P

L

S

S

A

E

M

T

F

A

M

K

H

H

Y

F

I

I

G

A

G

G

L

I

Q

V

R

K

Y

H

I

A

P

T

A

S

A

F

M

T

V

A

L

V

V

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

S

V

R

P

N
x
G

T
x
Y

A
x
E

A

A

P

P

I

C

N

Y

I

V

E

A

W

W

G

S

Y

A

D

Q

N

N

R
|
R

T

S

V

P

G

L

I

I

R

R

S

I

P

P

I

A

S

S

T

R

P

G

A

I

A

S

R

T

R

R

V

V

E
|
E

N

V

R
|
R

V

S

I

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

V

L

A

A

M

L

A

S

M

V

T

L

L

L

A

A

C

A

G

G

Y

L

L

D

G

G

I

I

M

K

N

N

K

K

I

L

E

E

P

A

K

P

A

A

E

P

M

I

K

D

G

R

D

N

A

I

Y
|
Y

L

V

C

M

A

S

Y

K

A

E

-

E

-

R

-

M

-

E

-

N

-

G

-

I

-

V

-

D

L

L

P

P

R

A

S

T

L

L

A

A

E

E

A

A

L

L

D

E

W

E

L

F

R

K

K

S

E

N

T

E

D

V

L

M

H

V

E

K

V

A

L

L

G

G

K

E

E

H

F

L

V

F

T

E

V
x
H

Y

F

S

I

E

E

I

A

K

K

E

E

L
x
I

E

E

F

W

D

D

E
x
M

F

F

M

R

K

T

V

Q

I

V

S

H

P

P

W

W

E

E

R

R

E

E

H

Q

L

Y

L

M

active site: D56 (≠ M61), E135 (= E145), E137 (= E147), E192 (= E210), E199 (= E217), H248 (= H266), R319 (= R337), E336 (= E354), R338 (= R356)
binding glutamine: E137 (= E147), E192 (= E210), R301 (= R319), E307 (≠ A325)
binding magnesium ion: I66 (≠ P79), E135 (= E145), E135 (= E145), E199 (= E217), H248 (= H266), H248 (= H266), E336 (= E354), H419 (≠ V428)
binding : F63 (≠ V76), V64 (≠ I77), R65 (≠ D78), I66 (≠ P79), D161 (≠ S179), G241 (= G259), V242 (≠ E260), N243 (≠ P261), G305 (≠ N323), Y306 (≠ T324), Y376 (= Y394), I426 (≠ L435), M430 (≠ E439)

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
29% identity, 97% coverage: 12:453/456 of query aligns to 7:440/443 of 4lnkA

query
sites
4lnkA

D

D

L

I

E

E

Q

K

W

L

L

V

N

K

E

E

R

E

H

N

V

V

T

K

E

Y

V

I

E

R

C

L

L

Q

V

F

P

T

D

D

L

I

T

L

G

G

V

T

A

I

R

K

G

N

K

V

I

E

L

I

P

P

R

V

A

S

K

Q

F

L

T

-

E

-

D

-

R

-

G

G

M
x
K

R

A

L

L

P

D

Q

N

A

K

I

V

V
x
M

A

F

M
x
D

G

G

V

S

T

S

G

I

E

E

F

-

P

-

Q

-

S

-

G

-

P

-

Y

-

D

G

V

F

I

V

D

R

P

I

T

E

D

E

K

S

D

D

M

M

Q

Y

L

L

R

Y

P

P

D

D

P

L

S

N

S

T

V

F

R

V

I

I

V

F

P

P

W

W

A

T

T

A

D

E

P

K

-

G

-

K

T

V

A

A

Q

R

V

F

I

I

H

C

D

D

C

I

F

Y

D

N

N

P

E

D

G

G

N

T

M

P

V

F

P

E

F

G

A

D

P

P

R

R

S

N

V

N

L

L

R

K

R

R

V

I

L

L

G

K

L

E

F

M

A

E

A

D

Q

L

G

G

L

F

Q

S

P

D

I

F

-

N

V

L

A

G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

T

F

A

-

R

-

N

K

T

L

D

D

P

E

N

K

M

G

L

E

P

P

R

T

P

L

P

E

I

L

G

N

R

D

S

K

G

G

R

-

A

-

E

-

T

-

S

-

R

-

Q

G

A

Y

Y

F

S

D

I

L

D

A

A

P

V

T

N

D

E

L

F

G

D

E

P

N

L

C

F

R

E

R

E

D

I

I

Y

V

D

L

F

E

S

L

D

E

K

E

M

M

E

G

L

F

N

E

V

I

D

E

T

A

L

S

I

H

H

H

E
|
E

V
|
V

G

A

A

P

G

G

Q

Q

M

H

E
|
E

I

I

N

D

F
|
F

F

K

H
x
Y

N

A

V

G

P

A

L

V

G

R

L

S

A

C

D

D

E

D

V

I

F

Q

F

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

S

I

A

A

L

R

R

K

H

H

D

G

M

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

S

F

G

G

E

V

P
x
N

G
|
G

S

S

A
x
G

M

M

H
|
H

V

C

H
x
N

Q

L

S
|
S

L

L

L

F

D

-

V

-

A

K

S

N

G

G

K

V

N

N

V

A

F

F

-

F

-

D

S

E

N

N

E

A

D

D

G

L

T

Q

P

L

S

S

A

E

M

T

F

A

M

K

H

H

Y

F

I

I

G

A

G

G

L

I

Q

V

R

K

Y

H

I

A

P

T

A

S

A

F

M

T

V

A

L

V

V

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

S

V

R

P

N

G

T

Y

A
x
E

A

A

P

P

I

C

N

Y

I

V

E

A

W

W

G

S

Y

A

D

Q

N

N

R
|
R

T

S

V

P

G

L

I

I

R

R

S

I

P

P

I

A

S
|
S

T

R

P

G

A

I

A
x
S

R

T

R
|
R

V

V

E
|
E

N

V

R
|
R

V

S

I

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

V

L

A

A

M

L

A

S

M

V

T

L

L

L

A

A

C

A

G

G

Y

L

L

D

G

G

I

I

M

K

N

N

K

K

I

L

E

E

P

A

K

P

A

A

E

P

M

I

K

D

G

R

D

N

A

I

Y

Y

L

V

C

M

A

S

Y

K

A

E

-

E

-

R

-

M

-

E

-

N

-

G

-

I

-

V

-

D

L

L

P

P

R

A

S

T

L

L

A

A

E

E

A

A

L

L

D

E

W

E

L

F

R

K

K

S

E

N

T

E

D

V

L

M

H

V

E

K

V

A

L

L

G

G

K

E

E

H

F

L

V

F

T

E

V

H

Y

F

S

I

E

E

I

A

K

K

E

E

L

I

E

E

F

W

D

D

E

M

F

F

M

R

K

T

V

Q

I

V

S

H

P

P

W

W

E

E

R

R

E

E

H

Q

L

Y

L

M

active site: D52 (≠ M61), E131 (= E145), E133 (= E147), E188 (= E210), E195 (= E217), H244 (= H266), R315 (= R337), E332 (= E354), R334 (= R356)
binding adenosine-5'-diphosphate: K43 (≠ M52), M50 (≠ V59), F198 (= F220), Y200 (≠ H222), N246 (≠ H268), S248 (= S270), S324 (= S346), S328 (≠ A350), R330 (= R352)
binding glutamic acid: E133 (= E147), E188 (= E210), V189 (= V211), N239 (≠ P261), G240 (= G262), G242 (≠ A264), E303 (≠ A325)
binding magnesium ion: E131 (= E145), E188 (= E210), E195 (= E217), H244 (= H266), E332 (= E354)

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
29% identity, 97% coverage: 12:453/456 of query aligns to 7:440/443 of 4lniA

query
sites
4lniA

D

D

L

I

E

E

Q

K

W

L

L

V

N

K

E

E

R

E

H

N

V

V

T

K

E

Y

V

I

E

R

C

L

L

Q

V

F

P

T

D

D

L

I

T

L

G

G

V

T

A

I

R

K

G

N

K

V

I

E

L

I

P

P

R

V

A

S

K

Q

F

L

T

-

E

-

D

-

R

-

G

G

M

K

R

A

L

L

P

D

Q

N

A

K

I

V

V

M

A

F

M
x
D

G

G

V

S

T

S

G

I

E

E

F

-

P

-

Q

-

S

-

G

-

P

-

Y

-

D

G

V

F

I

V

D

R

P

I

T

E

D

E

K

S

D

D

M

M

Q

Y

L

L

R

Y

P

P

D

D

P

L

S

N

S

T

V

F

R

V

I

I

V

F

P

P

W

W

A

T

T

A

D

E

P

K

-

G

-

K

T

V

A

A

Q

R

V

F

I

I

H

C

D

D

C

I

F

Y

D

N

N

P

E

D

G

G

N

T

M

P

V

F

P

E

F

G

A

D

P

P

R

R

S

N

V

N

L

L

R

K

R

R

V

I

L

L

G

K

L

E

F

M

A

E

A

D

Q

L

G

G

L

F

Q

S

P

D

I

F

-

N

V

L

A

G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

T

F

A

-

R

-

N

K

T

L

D

D

P

E

N

K

M

G

L

E

P

P

R

T

P

L

P

E

I

L

G

N

R

D

S

K

G

G

R

-

A

-

E

-

T

-

S

-

R

-

Q

G

A

Y

Y

F

S

D

I

L

D

A

A

P

V

T

N

D

E

L

F

G

D

E

P

N

L

C

F

R

E

R

E

D

I

I

Y

V

D

L

F

E

S

L

D

E

K

E

M

M

E

G

L

F

N

E

V

I

D
x
E

T

A

L

S

I

H

H

H

E
|
E

V

V

G

A

A

P

G

G

Q

Q

M

H

E
|
E

I

I

N
x
D

F

F

F

K

H
x
Y

N

A

V

G

P

A

L

V

G

R

L

S

A

C

D

D

E

D

V

I

F

Q

F

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

S

I

A

A

L

R

R

K

H

H

D

G

M

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

S

F

G

G

E

V

P

N

G

G

S

S

A

G

M

M

H
|
H

V

C

H
x
N

Q

L

S
|
S

L

L

L

F

D

-

V

-

A

K

S

N

G

G

K

V

N

N

V

A

F

F

-

F

-

D

S

E

N

N

E

A

D

D

G

L

T

Q

P

L

S

S

A

E

M

T

F

A

M

K

H

H

Y

F

I

I

G

A

G

G

L

I

Q

V

R

K

Y

H

I

A

P

T

A

S

A

F

M

T

V

A

L

V

V

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

S

V

R

P

N

G

T

Y

A
x
E

A

A

P

P

I

C

N

Y

I

V

E

A

W

W

G

S

Y

A

D

Q

N

N

R
|
R

T

S

V

P

G

L

I

I

R
|
R

S

I

P

P

I

A

S

S

T

R

P

G

A

I

A

S

R

T

R
|
R

V

V

E
|
E

N

V

R
|
R

V

S

I

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

V

L

A

A

M

L

A

S

M

V

T

L

L

L

A

A

C

A

G

G

Y

L

L

D

G

G

I

I

M

K

N

N

K

K

I

L

E

E

P

A

K

P

A

A

E

P

M

I

K

D

G

R

D

N

A

I

Y

Y

L

V

C

M

A

S

Y

K

A

E

-

E

-

R

-

M

-

E

-

N

-

G

-

I

-

V

-

D

L

L

P

P

R

A

S

T

L

L

A

A

E

E

A

A

L

L

D

E

W

E

L

F

R

K

K

S

E

N

T

E

D

V

L

M

H

V

E

K

V

A

L

L

G

G

K

E

E

H

F

L

V

F

T

E

V

H

Y

F

S

I

E

E

I

A

K

K

E

E

L

I

E

E

F

W

D

D

E

M

F

F

M

R

K

T

V

Q

I

V

S

H

P

P

W

W

E

E

R

R

E

E

H

Q

L

Y

L

M

active site: D52 (≠ M61), E131 (= E145), E133 (= E147), E188 (= E210), E195 (= E217), H244 (= H266), R315 (= R337), E332 (= E354), R334 (= R356)
binding adenosine-5'-diphosphate: E131 (= E145), E183 (≠ D205), D197 (≠ N219), Y200 (≠ H222), N246 (≠ H268), S248 (= S270), R320 (= R342), R330 (= R352)
binding magnesium ion: E131 (= E145), E131 (= E145), E133 (= E147), E188 (= E210), E195 (= E217), E195 (= E217), H244 (= H266), E332 (= E354)
binding l-methionine-s-sulfoximine phosphate: E133 (= E147), E188 (= E210), H244 (= H266), R297 (= R319), E303 (≠ A325), R315 (= R337), R334 (= R356)

P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 5 papers)
29% identity, 97% coverage: 12:453/456 of query aligns to 8:441/444 of P12425

query
sites
P12425

D

D

L

I

E

E

Q

K

W

L

L

V

N

K

E

E

R

E

H

N

V

V

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K

E

Y

V

I

E

R

C

L

L

Q

V

F

P

T

D

D

L

I

T

L

G

G

V

T

A

I

R

K

G

N

K

V

I

E

L

I

P

P

R

V

A

S

K

Q

F

L

T

-

E

-

D

-

R

-

G

G

M

K

R

A

L

L

P

D

Q

N

A

K

I

V

V

M

A

F

M

D

G

G

V

S

T

S

G

I

E

E

F

-

P

-

Q

-

S

-

G

-

P

-

Y

-

D
x
G

V

F

I

V

D
x
R

P

I

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E

D

E

K

S

D

D

M

M

Q

Y

L

L

R

Y

P

P

D

D

P

L

S

N

S

T

V

F

R

V

I

I

V

F

P

P

W

W

A

T

T

A

D

E

P

K

-

G

-

K

T

V

A

A

Q

R

V

F

I

I

H

C

D

D

C

I

F

Y

D

N

N

P

E

D

G

G

N

T

M

P

V

F

P

E

F

G

A

D

P

P

R

R

S

N

V

N

L

L

R

K

R

R

V

I

L

L

G

K

L

E

F

M

A

E

A

D

Q

L

G

G

L

F

Q

S

P

D

I

F

-

N

V

L

A

G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

T

F

A

-

R

-

N

K

T

L

D

D

P

E

N

K

M

G

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E

P

P

R

T

P

L

P

E

I

L

G

N

R

D

S

K

G

G

R

-

A

-

E

-

T

-

S

-

R

-

Q

G

A

Y

Y

F

S

D

I

L

D

A

A

P

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T

N

D

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D

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M

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E

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V

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A

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M

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E

I

I

N

D

F

F

F

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H

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P

A

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A

C

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F

M

M

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G

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G
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G

S

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H
|
H

V

C

H

N

Q

L

S

S

L

L

L

F

D

-

V

-

A

K

S

N

G

G

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V

N

N

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A

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F

-

F

-

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N

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A

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A

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A

F

M

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A

L

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A

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P

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Y

T

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V

N

N

S

S

Y

Y

R

K

R

R

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x
G

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A
x
E

A

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P
|
P

I

C

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N

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G

A

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R

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E

N

V

R

R

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G

D

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D

A

A

A

N

N

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P

Y

Y

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A

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T

L

L

L

A

A

C

A

G

G

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L

L

D

G

G

I

I

M

K

N

N

K

K

I

L

E

E

P

A

K

P

A

A

E

P

M

I

K

D

G

R

D

N

A

I

Y

Y

L

V

C

M

A

S

Y

K

A

E

-

E

-

R

-

M

-

E

-

N

-

G

-

I

-

V

-

D

L

L

P

P

R

A

S

T

L

L

A

A

E

E

A

A

L

L

D

E

W

E

L

F

R

K

K

S

E

N

T

E

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L

M

H

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V

A

L

L

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G

K

E

E

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L

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F

T

E

V

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Y

F

S

I

E

E

I

A

K

K

E

E

L

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E
|
E

F

W

D

D

E

M

F

F

M

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R

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M

G59 (≠ D75) mutation to R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
R62 (≠ D78) Important for inhibition by glutamine; mutation to A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.
E132 (= E145) binding Mg(2+)
E134 (= E147) binding Mg(2+)
E189 (= E210) binding Mg(2+)
V190 (= V211) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E217) binding Mg(2+)
G241 (= G262) binding L-glutamate
H245 (= H266) binding Mg(2+)
G302 (≠ N323) mutation to E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
E304 (≠ A325) mutation to A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
P306 (= P327) mutation to H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
E333 (= E354) binding Mg(2+)
E424 (= E436) mutation to K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

7tfaB Glutamine synthetase (see paper)
32% identity, 82% coverage: 81:453/456 of query aligns to 63:438/441 of 7tfaB

query
sites
7tfaB

D

E

K

S

D

D

M

M

Q

Y

L

L

R

Y

P

P

D

D

P

L

S

D

S

T

V

W

R

V

I

V

V

F

P

P

W

W

A

V

T

T

-

S

D

D

P

R

T

V

A

A

Q

R

V

L

I

I

H

C

D

D

C

I

F

Y

D

K

N

P

E

D

G

G

N

S

M

-

V

-

P

P

F

F

A

A

-

G

-

D

P

P

R

R

S

G

V

I

L

L

R

K

R

R

V

V

L

L

G

K

L

E

F

A

A

E

Q

L

G

G

L

Y

Q

T

P

S

I

M

-

N

V

V

A

G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

T

F

A

-

R

-

N

K

T

T

D

D

P

E

N

K

M

G

L

D

P

P

R

T

P

T

P

E

I

L

G

N

R

D

S

Q

G

G

R

-

A

-

E

-

T

-

S

-

R

-

Q

G

A
x
Y

Y

F

S

D

I

L

D

A

A

P

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M

N

D

E

L

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M

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A

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E

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E

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G

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H

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Q

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D

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A

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M

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A

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N

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Y

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R
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R

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L

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V

R

P

N
x
G

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x
Y

A
x
E

A

A

P

P

I

C

N

Y

I

V

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A

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W

G

S

Y

A

D

S

N

N

R
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R

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S

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M

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I

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R

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I

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P

I

A

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S

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R

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G

A

L

A

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R

R

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E
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E

N

V

R

R

V

N

I

P

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

V

L

A

A

M

L

A

A

M

V

T

M

L

L

A

R

C

A

G

G

Y

L

L

D

G

G

I

I

M

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N

R

K

Q

I

M

E

A

P

L

K

P

A

A

E

P

M

I

K

D

G

R

D

N

A

I

Y

Y

L

V

C

M

A

S

Y

E

-

E

-

R

-

I

-

E

-

G

-

I

-

P

A

S

L

L

P

P

R

A

S

D

L

L

A

K

E

E

A

A

L

L

D

S

W

E

L

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I

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R

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T

E

D

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L

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D

V

A

L

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G

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D

E

H

F

A

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A

V

Y

Y

F

S

Y

E

E

I

L

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K

E

E

L

I

E

E

F

W

D

D

E
x
M

F

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M

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W

E

E

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R

E

D

H

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L

L

binding glutamine: E131 (= E147), Y153 (≠ A177), E186 (= E210), G238 (= G262), H242 (= H266), R295 (= R319), E301 (≠ A325)
binding magnesium ion: E129 (= E145), E131 (= E147), E186 (= E210), E193 (= E217), H242 (= H266), E330 (= E354)
binding : V187 (= V211), N237 (≠ P261), G299 (≠ N323), Y300 (≠ T324), R313 (= R337), M424 (≠ E439)

Sites not aligning to the query:

binding : 58, 60

7tdpA Structure of paenibacillus polymyxa gs bound to met-sox-p-adp (transition state complex) to 1.98 angstom (see paper)
32% identity, 82% coverage: 81:453/456 of query aligns to 63:436/439 of 7tdpA

query
sites
7tdpA

D

E

K

S

D

D

M

M

Q

Y

L

L

R

Y

P

P

D

D

P

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A

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D

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R

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V

A

A

Q

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I

H

C

D

D

C

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D

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P

E

D

G

G

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-

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-

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F

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A

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G

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L

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A

A

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G

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x
N

V

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A
x
G

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P

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E

L

P

E
|
E

F

F

Y

F

L

L

T

F

A

-

R

-

N

K

T

T

D

D

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T

P

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E

I

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R

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G

G

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-

A

-

E

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-

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R

-

Q

G

A

Y

Y

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I

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E
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E

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G

A

A

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G

G

Q

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M

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E
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E

I

I

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x
D

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x
Y

N

A

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A

L

V

G

K

L

A

A

A

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K

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V

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A

A

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H

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L

Y

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A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

S

F

G

G

E

V

P

N

G
|
G

S

S

A

G

M

M

H
|
H

V

C

H
x
N

Q

Q

S
|
S

L

L

L

F

D

-

V

-

A

K

S

D

G

N

K

E

N

N

V

V

F

F

S

Y

N

D

E

E

D

T

G

D

T

E

P

L

-

G

-

L

S

S

A

Q

M

T

F

A

M

R

H

H

Y

Y

I

M

G

A

G

G

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I

Q

L

R

K

Y

H

I

A

P

R

A

A

A

M

M

A

V

A

L

I

V

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

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R
|
R

L

L

S

V

R

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N

G

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Y

A
x
E

A

A

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P

I

C

N

Y

I

V

E

A

W

W

G

S

Y

A

D

S

N

N

R
|
R

T

S

V

P

G

M

I

I

R
|
R

S

I

P

P

I

A

S

S

T

R

P

G

A

L

A

S

R

T

R
|
R

V

V

E
|
E

N

V

R
|
R

V

N

I

P

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

V

L

A

A

M

L

A

A

M

V

T

M

L

L

A

R

C

A

G

G

Y

L

L

D

G

G

I

I

M

K

N

R

K

Q

I

M

E

A

P

L

K

P

A

A

E

P

M

I

K

D

G

R

D

N

A

I

Y

Y

L

V

C

M

A

S

Y

E

-

E

-

R

-

I

-

E

-

G

-

I

-

P

A

S

L

L

P

P

R

A

S

D

L

L

A

K

E

E

A

A

L

L

D

S

W

E

L

L

R

I

K

R

E

S

T

E

D

V

L

I

H

S

E

D

V

A

L

L

G

G

K

D

E

H

F

A

V

L

T

A

V

Y

Y

F

S

Y

E

E

I

L

K

K

E

E

L

I

E

E

F

W

D

D

E

M

F

Y

M

R

K

T

V

Q

I

V

S

H

P

Q

W

W

E

E

R

R

E

D

H

Q

L

Y

L

L

binding adenosine-5'-diphosphate: N123 (vs. gap), G125 (≠ A143), E127 (= E145), E179 (≠ D205), D193 (≠ N219), Y196 (≠ H222), N242 (≠ H268), S244 (= S270), R316 (= R342), R326 (= R352)
binding magnesium ion: E127 (= E145), E127 (= E145), E129 (= E147), E184 (= E210), E191 (= E217), E191 (= E217), H240 (= H266), E328 (= E354)
binding l-methionine-s-sulfoximine phosphate: E127 (= E145), E129 (= E147), E184 (= E210), E191 (= E217), G236 (= G262), H240 (= H266), R293 (= R319), E299 (≠ A325), R311 (= R337), R330 (= R356)

7tf9S L. Monocytogenes gs(14)-q-glnr peptide (see paper)
28% identity, 97% coverage: 12:453/456 of query aligns to 7:440/443 of 7tf9S

query
sites
7tf9S

D

D

L

I

E

F

Q

R

W

F

L

A

N

D

E

E

R

Q

H

N

V

V

T

K

E

F

V

I

E

R

C

L

L

Q

V

F

P

T

D

D

L

I

T

L

G

G

V

I

A

I

R

K

G

N

K

V

I

E

L

I

P

P

R

V

A

S

K

Q

F

L

T

K

E

K

D

-

R

-

G

-

M

-

R

A

L

L

P

D

Q

N

A

K

I

I

V

M

A

F

M

D

G

G

V

S

T

S

G

I

E

E

F

-

P

-

Q

-

S

-

G

-

P

-

Y

-

D

G

V
x
F

I
x
V

D

R

P

I

T

E

D

E

K

S

D

D

M

M

Q

Y

L

L

R

F

P

P

D

D

P

L

S

D

S

T

V

W

R

V

I

V

V

F

P

P

W

W

A

T

T

A

D

E

P

K

-

G

-

K

T

V

A

A

Q

R

V

M

I

I

H

C

D

D

C

I

F

Y

D

N

N

P

E

-

G

-

N

D

M

M

V

T

P

P

F

F

A

A

-

G

-

D

P

P

R

R

S

A

V

N

L

L

R

K

R

R

V

V

L

L

G

K

L

E

F

M

A

E

Q

L

G

G

L

F

Q

T

P

E

I

F

-

N

V

L

A

G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

T

F

A

-

R

-

N

K

T

L

D

D

P

E

N

N

M

R

L

R

P

P

R

T

P

L

P

E

I

L

G

N

R

D

S

S

G

G

R

-

A

-

E

-

T

-

S

-

R

-

Q

G

A
x
Y

Y

F

S

D

I

L

D

A

A

P

V

T

N

D

E

L

F

G

D

E

P

N

L

C

F

R

E

R

E

D

I

I

Y

V

D

L

F

E

S

L

D

E

K

E

M

M

E

G

L

F

N

E

V

I

D

E

T

A

L

S

I

H

H

H

E
|
E

V

V

G

A

A

P

G

G

Q

Q

M

H

E
|
E

I

I

N

D

F

F

F

K

H

Y

N

E

V

D

P

A

L

I

G

T

L

A

A

C

D

D

E

S

V

I

F

Q

F

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

S

I

A

A

L

R

R

K

H

H

D

G

M

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

S

F

G

G

E

V

P

N

G
|
G

S

S

A
x
G

M

M

H
|
H

V

F

H

N

Q

M

S

S

L

L

L

F

D

N

V

E

A

K

S

G

G

-

K

-

N

N

V

A

F

F

S

F

N

D

E

E

D

S

G

G

T

E

P

L

-

E

-

L

S

S

A

Q

M

T

F

A

M

Y

H

H

Y

F

I

L

G

A

G

G

L

M

Q

L

R

K

Y

H

I

A

P

R

A

G

A

Y

M

T

V

A

L

V

V

T

A

N

P

P

Y

T

V

I

N

N

S

S

Y

F

R

K

R
|
R

L

L

S

V

R

P

N

G

T

Y

A
x
E

A

A

P

P

I

C

N

Y

I

I

E

A

W

W

G

S

Y

G

D

K

N

N

R

R

T

S

V

P

G

L

I

V

R

R

S

V

P

P

I

S

S

S

T

R

P

G

A

L

A

S

R

T

R

R

V

L

E
|
E

N

L

R

R

V

S

I

V

G

D

A

P

D

S

A

A

N

N

P

P

Y

Y

V

L

A

A

M

M

A

A

M

V

T

L

L

L

A

K

C

A

G

G

Y

L

L

S

G

G

I

I

M

K

N

D

K

E

I

L

E

T

P

P

K

P

A

A

E

P

M

V

K

D

G

R

D

N

A

I

Y

Y

-

G

-

M

-

N

-

E

-

R

-

E

-

A

L

T

C

G

A

I

Y

Y

A

D

L

L

P

P

R

E

S

S

L

L

A

G

E

H

A

A

L

L

D

I

W

E

L

L

R

E

K

K

E

N

T

E

D

I

L

I

H

K

E

D

V

G

L

L

G

G

K

E

E

H

F

I

V

F

T

E

V

H

Y

F

S

I

E
|
E

I

A

K

K

E

T

L
x
I

E

E

F

C

D

D

E
x
M

F

F

M

R

K

T

V

A

I

V

S

H

P

P

W

W

E

E

R

R

E

E

H

Q

L

Y

L

L

binding glutamine: E133 (= E147), Y155 (≠ A177), E188 (= E210), G240 (= G262), G242 (≠ A264), R297 (= R319), E303 (≠ A325)
binding magnesium ion: E131 (= E145), E133 (= E147), E188 (= E210), E195 (= E217), H244 (= H266), E332 (= E354)
binding : F59 (≠ V76), V60 (≠ I77), E418 (= E431), I422 (≠ L435), M426 (≠ E439)

7tenA Glutamine synthetase (see paper)
28% identity, 97% coverage: 12:453/456 of query aligns to 6:439/442 of 7tenA

query
sites
7tenA

D

D

L

I

E

F

Q

R

W

F

L

A

N

D

E

E

R

Q

H

N

V

V

T

K

E

F

V

I

E

R

C

L

L

Q

V

F

P

T

D

D

L

I

T

L

G

G

V

I

A

I

R

K

G

N

K

V

I

E

L

I

P

P

R

V

A

S

K

Q

F

L

T

K

E

K

D

-

R

-

G

-

M

-

R

A

L

L

P

D

Q

N

A

K

I

I

V

M

A

F

M

D

G

G

V

S

T

S

G

I

E

E

F

-

P

-

Q

-

S

-

G

-

P

-

Y

-

D

G

V

F

I

V

D

R

P

I

T

E

D

E

K

S

D

D

M

M

Q

Y

L

L

R

F

P

P

D

D

P

L

S

D

S

T

V

W

R

V

I

V

V

F

P

P

W

W

A

T

T

A

D

E

P

K

-

G

-

K

T

V

A

A

Q

R

V

M

I

I

H

C

D

D

C

I

F

Y

D

N

N

P

E

-

G

-

N

D

M

M

V

T

P

P

F

F

A

A

-

G

-

D

P

P

R

R

S

A

V

N

L

L

R

K

R

R

V

V

L

L

G

K

L

E

F

M

A

E

Q

L

G

G

L

F

Q

T

P

E

I

F

-

N

V

L

A
x
G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

T

F

A

-

R

-

N

K

T

L

D

D

P

E

N

N

M

R

L

R

P

P

R

T

P

L

P

E

I

L

G

N

R

D

S

S

G

G

R

-

A

-

E

-

T

-

S

-

R

-

Q

G

A

Y

Y

F

S

D

I

L

D

A

A

P

V

T

N

D

E

L

F

G

D

E

P

N

L

C

F

R

E

R

E

D

I

I

Y

V

D

L

F

E

S

L

D

E

K

E

M

M

E

G

L

F

N

E

V

I

D
x
E

T

A

L

S

I

H

H

H

E
|
E

V

V

G

A

A

P

G

G

Q

Q

M

H

E
|
E

I

I

N
x
D

F
|
F

F
x
K

H
x
Y

N

E

V

D

P

A

L

I

G

T

L

A

A

C

D

D

E

S

V

I

F

Q

F

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

S

I

A

A

L

R

R

K

H

H

D

G

M

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

S

F

G

G

E

V

P
x
N

G
|
G

S

S

A

G

M

M

H
|
H

V

F

H
x
N

Q

M

S
|
S

L

L

L

F

D

N

V

E

A

K

S

G

G

-

K

-

N

N

V

A

F

F

S

F

N

D

E

E

D

S

G

G

T

E

P

L

-

E

-

L

S

S

A

Q

M

T

F

A

M

Y

H

H

Y

F

I

L

G

A

G

G

L

M

Q

L

R

K

Y

H

I

A

P

R

A

G

A

Y

M

T

V

A

L

V

V

T

A

N

P

P

Y

T

V

I

N

N

S

S

Y

F

R

K

R
|
R

L

L

S

V

R

P

N

G

T

Y

A
x
E

A

A

P

P

I

C

N

Y

I

I

E

A

W

W

G

S

Y

G

D

K

N

N

R
|
R

T

S

V

P

G

L

I

V

R
|
R

S

V

P

P

I

S

S

S

T

R

P

G

A

L

A
x
S

R

T

R
|
R

V

L

E

E

N

L

R
|
R

V

S

I

V

G

D

A

P

D

S

A

A

N

N

P

P

Y

Y

V

L

A

A

M

M

A

A

M

V

T

L

L

L

A

K

C

A

G

G

Y

L

L

S

G

G

I

I

M

K

N

D

K

E

I

L

E

T

P

P

K

P

A

A

E

P

M

V

K

D

G

R

D

N

A

I

Y

Y

-

G

-

M

-

N

-

E

-

R

-

E

-

A

L

T

C

G

A

I

Y

Y

A

D

L

L

P

P

R

E

S

S

L

L

A

G

E

H

A

A

L

L

D

I

W

E

L

L

R

E

K

K

E

N

T

E

D

I

L

I

H

K

E

D

V

G

L

L

G

G

K

E

E

H

F

I

V

F

T

E

V

H

Y

F

S

I

E

E

I

A

K

K

E

T

L

I

E

E

F

C

D

D

E

M

F

F

M

R

K

T

V

A

I

V

S

H

P

P

W

W

E

E

R

R

E

E

H

Q

L

Y

L

L

binding adenosine-5'-diphosphate: G128 (≠ A143), E130 (= E145), E182 (≠ D205), D196 (≠ N219), F197 (= F220), K198 (≠ F221), Y199 (≠ H222), N245 (≠ H268), S247 (= S270), R319 (= R342), S327 (≠ A350), R329 (= R352)
binding l-methionine-s-sulfoximine phosphate: E130 (= E145), E132 (= E147), E187 (= E210), E194 (= E217), N238 (≠ P261), G239 (= G262), H243 (= H266), R296 (= R319), E302 (≠ A325), R314 (= R337), R333 (= R356)

5dm3A Crystal structure of glutamine synthetase from chromohalobacter salexigens dsm 3043(csal_0679, target efi-550015) with bound adp
32% identity, 80% coverage: 86:448/456 of query aligns to 52:368/374 of 5dm3A

query
sites
5dm3A

L

M

R

K

P

P

D

D

P

L

S

A

S

T

V

L

R

R

I

C

V

V

P

P

W

W

A

-

T

L

D

E

P

G

T

T

A

A

Q

M

V

V

I

L

H

C

D

D

C

L

F

L

D

H

N

A

E

E

G

-

N

-

M

-

V

V

P

P

F

H

A

A

P

P

R

R

S

A

V

I

L

L

R

K

R

R

V

Q

L

L

G

A

L

R

F

L

A

E

A

A

Q

M

G

G

L

L

Q

E

P

A

I
|
I

V

M

A

A

P

T

E
|
E

L

L

E
|
E

F

F

Y

F

L

L

T

F

A

E

R

K

N

S

T

L

D

D

P

-

N

-

M

-

L

-

P

-

R

-

P

-

I

-

G

-

R

-

S

-

G

-

R

-

A

-

E

-

T

T

S

T

R

K

Q

E

A

E

Y

H

S

V

I

L

D

R

A

-

V

-

N

-

E

-

F

-

D

-

P

P

L

L

F

R

E

N

E

H

I

L

Y

H

D

A

F

A

S

G

D

I

K

P

M

V

E
|
E

L

G

N

T

V

K

D

-

T

-

L

-

I

-

H

-

E

-

V

-

G

G

A
x
E

G

G

Q

Q

M

E

E
|
E

I

L

N

N

F

I

F
x
R

H
x
C

N

A

V

K

P

A

L

L

G

D

L

T

A

A

D

D

E

Y

V

H

F

T

F

I

F

A

K

K

R

H

T

A

V

T

R

K

E

E

S

I

A

A

L

W

R

Q

H

Q

D

G

M

R

Y

A

A

V

T

T

F

F

M

L

A

S

K

K

P

W

I

H

S

H

G

A

E

H

P

A

G

G

S

S

A

S

M

S

H
|
H

V

I

H
|
H

Q

Q

S
|
S

L

L

L

W

D

K

V

-

A

-

S

Q

G

G

K

L

N

P

V

A

F

F

S

H

N

D

E

E

D

R

G

D

T

A

-

L

-

G

P

M

S

S

A

A

M

L

F

M

M

K

H

H

Y

Y

I

L

G

A

G

G

L

L

Q

L

R

K

Y

Y

I

A

P

P

A

D

A

Y

M

T

V

Y

L

F

V

L

A

A

P

P

Y

Y

V

L

N

N

S

S

Y

Y

R

K

R

R

L

F

S

Q

R

-

N

-

T

-

A

-

P

P

I

T

N

R

I

T

E

V

W

W

G

S

Y

V

D

D

N

N

R
|
R

T

T

V

A

G

G

I

F

R

R

S

L

P

C

I

A

S

E

T

G

P

T

A

R

A

A

R

V

R
|
R

V

I

E
|
E

N

C

R
|
R

V

I

I

G

G

G

A

S

D

D

A

L

N

N

P

P

Y

Y

V

L

A

A

M

M

A

A

M

G

T

Q

L

L

A

A

C

A

G

G

Y

I

L

K

G

G

I

I

M

E

N

E

K

C

I

L

E

A

-

L

-

P

P

P

K

P

A

A

E

G

M

L

K

-

G

-

D

-

A

-

Y

-

L

-

C

-

A

-

Y

-

A

-

L

I

P

P

R

Q

S

N

L

L

A

R

E

D

A

A

L

M

D

E

W

A

L

L

R

R

K

G

E

S

T

T

D

M

L

L

H

R

E

E

V

A

L

M

G

G

K

E

E

D

F

V

V

V

T

D

V

H

Y

Y

S

V

E

R

I

A

K

A

E

E

L

V

E

E

F

L

D

E

E

D

F

F

M

Q

K

R

V

V

I

V

S

S

P

D

W

Y

E

E

active site: E107 (= E145), E109 (= E147), E146 (≠ A213), E150 (= E217), H199 (= H266), R265 (= R337), E282 (= E354), R284 (= R356)
binding adenosine-5'-diphosphate: I103 (= I141), E141 (= E201), R154 (≠ F221), C155 (≠ H222), H201 (= H268), S203 (= S270), R280 (= R352)

A0R079 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
30% identity, 92% coverage: 1:421/456 of query aligns to 1:437/478 of A0R079

query
sites
A0R079

M

M

S

A

E

E

K

K

N

T

K

S

P

-

M

-

N

-

F

-

N

D

D

D

L

I

E

F

Q

K

W

L

L

I

N
x
K

E

D

R

E

H

N

V

V

T

E

E

Y

V

V

E

D

C

I

L

R

V

F

P

C

D

D

L

L

T

P

G

G

V

V

A

V

R

Q

G

H

K

F

I

S

L

I

P

P

R

A

A

S

K

A

F

F

T

D

E

E

D

-

R

-

G

-

M

-

R

-

L

-

P

-

Q

-

A

S

I

V

V

F

A

E

M

D

G

G

V

L

T

A

G

F

E

D

F

G

P

S

Q

S

S

V

G

R

P

G

Y

F

Y

Q

D

S

V

I

I

-

D

-

P

-

T

H

D

E

K

S

D

D

M

M

Q

M

L

L

R

L

P

P

D

D

P

P

S

N

S

T

V

A

R

R

I

I

V

D

P

P

W

F

A

R

T

A

D

A

P

K

T

T

A

L

Q

N

V

M

-

N

-

F

I

V

H

H

D

D

C

P

F

F

D

T

N

R

E

E

G

A

N

-

M

-

V

Y

P

S

F

R

A

D

P

P

R

R

S

N

V

V

L

A

R

R

R

K

V

A

L

E

G

N

L

Y

F

L

A

A

A

S

Q

T

G

G

L

I

-

A

-

D

Q

T

P

A

I

F

V

F

A

G

P

A

E

E

L

A

E

E

F

F

Y

Y

L

I

-

F

-

D

-

S

T

V

A

S

R

F

N

D

T

S

D

K

P

I

N

N

M

G

L

T

P

F

R

Y

P

E

P

V

I

D

G

S

R

E

S

S

G

G

R

W

A

W

E

N

T

T

-

G

-

E

-

P

-

F

-

E

-

S

-

D

-

G

-

S

S

A

R

N

Q

R

A

G

Y

Y

S

K

I

V

D

R

A

P

V

K

N

G

E

G

F

Y

D

F

P

P

L

V

-

A

-

P

F

Y

E

D

E

H

I

Y

Y

V

D

D

F

L

S

R

D

D

K

Q

M

M

E

A

L

T

N

N

V

L

D

Q

T

N

L

A

-

G

-

F

-

T

-

L

-

E

-

R

-

G

I

H

H

H

E

E

V

V

G

G

-

T

A

A

G

G

Q

Q

M

A

E

E

I

I

N

N

F

Y

F

K

H

F

N

N

V

T

P

L

L

L

G

A

L

A

A

A

D

D

E

D

V

V

F

L

F

F

K

K

R

Y

T

I

V

I

R

K

E

N

S

T

A

A

L

W

R

Q

H

A

D

G

M

K

Y

T

A

V

T

T

F

F

M

M

A

P

K

K

P

P

I

L

S

F

G

G

E

D

P

N

G

G

S

S

A

G

M

M

H

H

V

A

H

H

Q

Q

S

S

L

L

L

W

D

-

V

-

A

K

S

D

G

G

K

Q

N

P

V

L

F

F

S

H

N

D

E

E

D

S

G

G

T

Y

P

A

-

G

-

L

S

S

A

D

M

I

F

A

M

R

H

H

Y

Y

I

I

G

G

L

I

Q

L

R

H

Y

H

I

A

P

P

A

S

A

L

M

L

V

A

L

F

V

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

S

V

R

P

N

G

T

Y

A

E

A

A

P

P

I

I

N

N

I

L

E

V

W

Y

G

S

Y

Q

D

R

N

N

R

R

T

S

V

A

G

C

I

V

R

R

S

I

P

P

I

I

S

T

-

G

-

N

T

N

P

P

A

K

A

A

R

K

R

R

V

L

E

E

N

F

R

R

V

C

I

P

G

D

A

S

D

S

A

G

N

N

P

P

Y

Y

V

L

A

A

M

F

A

A

M

A

T

M

L

L

A

M

C

A

G

G

Y

I

L

D

G

G

I

I

M

K

N

K

K

K

I

I

E

E

P

P

K

L

A

Q

E

P

M

V

K

D

G

K

D

D

A

L

Y

Y

L

E

C

L

-

P

-

D

-

E

A

A

Y

A

A

A

L

I

P

P

R

Q

-

A

-

P

-

T

S

S

L

L

A

S

E

A

A

V

L

I

D

D

W

K

L

L

R

E

K

E

E

D

T

-

D

-

L

-

H

H

E

E

V

Y

L

L

K14 (≠ N18) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

8wwvA Glutamine synthetase (see paper)
30% identity, 90% coverage: 31:441/456 of query aligns to 38:464/490 of 8wwvA

query
sites
8wwvA

D

D

L

Q

T

Y

G

G

V

L

A

L

R

R

G

G

K

K

I

S

L

L

P

T

-

V

-

A

-

S

-

L

R

K

A

S

K

A

F

F

T

K

E

E

D

G

R

S

G

E

M

V

R

A

L

I

P

G

Q

P

-

F

A

D

I

L

V

V

A

S

M

S

G

M

V

V

T

F

G

N

E

L

F

F

P

T

Q

T

S

A

G

G

P

D

Y

F

Y

E

D

D

V

E

I

L

-

S

-

G

D

N

P

P

T

T

D

-

K

-

D

-

M

V

Q

V

L

M

R

V

P

P

D

D

P

P

S

T

V

F

R

K

I

V

V

L

P

P

W

W

A

A

T

-

D

D

P

K

T

T

A

G

Q

W

V

M

I

L

H

A

D

D

C

L

F

H

D

W

N

K

E

S

G

G

N

E

M

P

V

F

P

P

F

L

A

C

P

P

R

R

S

G

V

I

L

M

R

K

V

A

L

V

G

K

L

S

F

L

A

S

A

D

Q

E

G

G

L

Y

Q

L

P

F

I

K

V

C

A
x
G

P

I

E
|
E

L

L

E
|
E

F

W

Y

Y

L

L

T

T

A

K

-

I

-

V

-

D

R

R

N

S

T

L

D

S

P

P

N

E

M

S

L

L

P

G

R

A

P

P

P

G

I

V

G

Q

R

P

S

D

G

A

-

I

R

Q

A

V

E

Q

T

P

S

V

R

A

Q

Q

A

G

Y

Y

S

S

I

V

-

L

-

E

D

H

A

H

V

L

N

D

E

Q

F

V

D

D

P

D

L

I

F

M

E

S

E

K

I

V

Y

R

D

K

F

G

S

L

D

L

K

E

M

L

E

N

L

L

N

P

V

L

D
x
R

T

S

L

I

I

E

H

D

E
|
E

V

W

G

A

A

P

G

S

Q

Q

M

M

E
|
E

I

T

N

T

F
|
F

F
x
D

H
x
V

N

M

V

E

P

G

L

L

G

E

L

A

A

A

D

D

E

A

V

A

F

L

F

I

K

K

R

S

T

A

V

I

R

K

E

Q

S

I

A

C

L

S

R

R

H

H

D

G

M

Y

Y

H

A

A

T

T

F

F

M

M

A

C

K

K

P

P

-

A

I

I

S

N

G

G

E

F

P

F

G
x
A

S

S

A

G

M

W

H
|
H

V

M

H
|
H

Q

Q

S
|
S

L

L

L

V

D

D

V

K

A

D

S

T

G

R

K

K

N

N

V

L

F

F

S

I

N

P

E

S

D

E

G

G

-

E

T

V

P

L

S

S

A

P

M

L

F

G

M

R

H

A

Y

Y

I

A

G

G

L

L

Q

L

R

A

Y

N

I

G

P

S

A

A

M

S

V

S

L

F

V

T

A

T

P

P

Y

T

V

V

N

N

S

G

Y

Y

R

R

R
|
R

L

R

S

Q

R

P

N

Y

T

S

A

L

A

A

P

P

I

D

N

R

I

R

E

A

W

W

G

A

Y

K

D

D

N

N

R
x
K

T

A

V

A

G

M

I

V

R

R

-

V

S

S

P

A

I

T

S

G

T

D

P

P

A

A

A

S

R
|
R

R

-

V

I

E
|
E

N

N

R

R

V

I

I

G

G

E

A

P

D

G

A

A

N

N

P

P

Y

Y

V

L

A

Y

M

M

A

A

M

S

T

Q

L

I

A

V

C

S

G

G

Y

L

L

D

G

G

I

I

M

K

N

N

K

K

I

K

E

D

P

P

K

-

A

G

E

E

M

L

K

Q

G

E

D

S

A

P

Y

Y

L

D

C

A

A

Q

Y

V

-

P

A

M

L

L

P

P

R

T

S

T

L

L

A

A

E

E

A

A

L

L

D

D

W

A

L

L

R

E

K

H

E

D

T

S

D

E

L

L

-

F

H

R

E

S

V

C

L

F

G

G

K

D

E

T

F

F

V

I

T

K

V

Y

Y

W

S

L

E

Q

I

L

K

R

E

R

L

S

E

E

F

W

D

A

E

R

F

F

M

L

binding adenosine-5'-diphosphate: G155 (≠ A143), E157 (= E145), R224 (≠ D205), F239 (= F220), D240 (≠ F221), V241 (≠ H222), H288 (= H268), S290 (= S270), R374 (= R351), E376 (= E354)
binding magnesium ion: E157 (= E145), E236 (= E217)
binding manganese (ii) ion: E157 (= E145), E159 (= E147), E229 (= E210), E236 (= E217), H286 (= H266), E376 (= E354)
binding l-methionine-s-sulfoximine phosphate: E157 (= E145), E159 (= E147), E229 (= E210), E236 (= E217), A282 (≠ G262), H286 (= H266), R340 (= R319), K358 (≠ R337)

Query Sequence

>WP_011808619.1 NCBI__GCF_000015565.1:WP_011808619.1
MSEKNKPMNFNDLEQWLNERHVTEVECLVPDLTGVARGKILPRAKFTEDRGMRLPQAIVA
MGVTGEFPQSGPYYDVIDPTDKDMQLRPDPSSVRIVPWATDPTAQVIHDCFDNEGNMVPF
APRSVLRRVLGLFAAQGLQPIVAPELEFYLTARNTDPNMLPRPPIGRSGRAETSRQAYSI
DAVNEFDPLFEEIYDFSDKMELNVDTLIHEVGAGQMEINFFHNVPLGLADEVFFFKRTVR
ESALRHDMYATFMAKPISGEPGSAMHVHQSLLDVASGKNVFSNEDGTPSAMFMHYIGGLQ
RYIPAAMVLVAPYVNSYRRLSRNTAAPINIEWGYDNRTVGIRSPISTPAARRVENRVIGA
DANPYVAMAMTLACGYLGIMNKIEPKAEMKGDAYLCAYALPRSLAEALDWLRKETDLHEV
LGKEFVTVYSEIKELEFDEFMKVISPWEREHLLLHV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory