SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011811717.1 NCBI__GCF_000015565.1:WP_011811717.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P81600 Alcohol dehydrogenase class-3 chain H; Alcohol dehydrogenase class-III chain H; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Gadus morhua (Atlantic cod) (see 2 papers)
65% identity, 100% coverage: 1:369/369 of query aligns to 7:375/375 of P81600

query
sites
P81600

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Sites not aligning to the query:

1 modified: N-acetylalanine

P80467 Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Saara hardwickii (Indian spiny-tailed lizard) (Uromastyx hardwickii) (see paper)
65% identity, 100% coverage: 1:369/369 of query aligns to 5:373/373 of P80467

query
sites
P80467

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Sites not aligning to the query:

1 modified: N-acetylalanine

P81601 Alcohol dehydrogenase class-3 chain L; Alcohol dehydrogenase class-III chain L; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Gadus morhua (Atlantic cod) (see 2 papers)
64% identity, 100% coverage: 1:369/369 of query aligns to 7:375/375 of P81601

query
sites
P81601

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Sites not aligning to the query:

1 modified: N-acetylalanine

4dl9A Crystal structure of s-nitrosoglutathione reductase from tomato (solanum lycopersicum) in complex with NAD+ (see paper)
63% identity, 99% coverage: 4:367/369 of query aligns to 11:375/379 of 4dl9A

query
sites
4dl9A

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G

A

A

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N

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T

A

A

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K

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V

K

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P

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x
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x
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I

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active site: C47 (= C40), H48 (= H41), T49 (= T42), Y52 (≠ F45), H69 (= H62), E70 (= E63), C99 (= C92), C102 (= C95), C105 (= C98), C113 (= C106), R117 (= R110), C177 (= C169), T181 (= T173), R372 (= R364)
binding nicotinamide-adenine-dinucleotide: Y95 (= Y88), T181 (= T173), G204 (= G196), V206 (≠ I198), D226 (= D218), I227 (≠ T219), K231 (= K223), C271 (= C263), I272 (= I264), V295 (≠ I287), G296 (= G288), V297 (= V289), A321 (= A313), F322 (= F314)
binding zinc ion: C47 (= C40), H69 (= H62), E70 (= E63), C99 (= C92), C102 (= C95), C105 (= C98), C113 (= C106), C177 (= C169)

P81431 Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Octopus vulgaris (Common octopus) (see paper)
62% identity, 99% coverage: 1:367/369 of query aligns to 8:374/378 of P81431

query
sites
P81431

M

I

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A

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Sites not aligning to the query:

1 modified: N-acetylthreonine

P80360 Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Myxine glutinosa (Atlantic hagfish) (see 2 papers)
63% identity, 99% coverage: 4:368/369 of query aligns to 11:375/376 of P80360

query
sites
P80360

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A

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N

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A

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Q

Sites not aligning to the query:

1 modified: N-acetylserine

8co4C Crystal structure of apo s-nitrosoglutathione reductase from arabidopsis thalina (see paper)
62% identity, 99% coverage: 4:367/369 of query aligns to 11:375/379 of 8co4C

query
sites
8co4C

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A

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C

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C

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A

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-

I

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N

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G

G

Q

T

S

C

I

L

R

R

T

C

V

V

I

L

binding zinc ion: C47 (= C40), H69 (= H62), C99 (= C92), C102 (= C95), C105 (= C98), C113 (= C106), C177 (= C169)

Q96533 Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; Nitrosoglutathione reductase; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.-; EC 1.1.1.284 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
62% identity, 99% coverage: 4:367/369 of query aligns to 11:375/379 of Q96533

query
sites
Q96533

R

K

A

A

V

V

A

A

F

Y

E

E

A

P

G

N

K

K

P

P

L

L

Q

V

I

I

V

E

E

D

I

V

D

Q

V

V

E

A

P

P

R

Q

A

A

G

G

E

E

V

V

L

R

V

I

K

K

I

I

T

L

H

Y

T

T

G

A

V

L

C
|
C

H
|
H

T

T

D

D

A

A

F

Y

T

T

L

W

S

S

G

G

D

K

D

D

P

P

E

E

G

G

I

L

F

F

P

P

A

C

V

I

L

L

G

G

H
|
H

E
|
E

G

A

A

A

G

G

V

I

V

V

V

E

E

S

V

V

G

G

A

E

G

G

V

V

V

T

A

E

L

V

A

Q

P

A

G

G

D

D

H

H

V

V

I

I

P

P

L

C

Y

Y

T

Q

A

A

E

E

C
|
C

R

R

A

E

C
|
C

K

K

F

F

C
|
C

L

K

S

S

G

G

K

K

T

T

N

N

L

L

C
|
C

Q

G

K

K

V

V

R

R

A

S

T

A

Q

T

G

G

K

V

G

G

-

I

L

M

M

M

P

N

D

D

A

R

S

K

S

S

R

R

F

F

S

S

Y

V

Q

N

G

G

Q

K

P

P

I

I

F

Y

H

H

Y

F

M

M

G

G

C

T

S

S

T

T

F

F

S

S

E

Q

Y

Y

T

T

V

V

N

H

E

D

I

V

S

S

L

V

A

A

K

K

I

I

N

D

P

P

A

T

A

A

N

P

H

L

E

D

Q

K

V

V

C

C

L

L

G

G

C
|
C

G

G

V

V

T

P

T

T

G

G

L

L

G

G

A

A

V

V

K

W

N

N

T

T

A

A

K

K

V

V

K

E

P

P

G

G

D

S

T

N

V

V

A

A

V

I

F

F

G
|
G

L
|
L

G
|
G

G
x
T

I
x
V

G
|
G

L

L

A

A

V

V

I

A

Q

E

G

G

A

A

K

K

L

T

A

A

R

G

A

A

G

S

R

R

I

I

G

G

V

I

D
|
D

T

I

N

D

P

S

G

K

K
|
K

F

Y

D

E

L

T

A

A

R

K

T

K

M

F

G

G

A

V

T

N

D

E

C

F

V

V

N

N

P

P

K

K

D

D

H

H

A

D

K

K

P

P

I

I

Q

Q

D

E

V

V

I

I

V

V

E

D

M

L

T

T

D

D

G

G

V

V

E

D

F

Y

S

S

F

F

E

E

C

C

I
|
I

G

G

N

N

V

V

D

S

V

V

M

M

R

R

A

A

L

L

E

E

C

C

H

H

K

K

G

G

W

W

G

G

E

T

S

S

V

V

I

I

I
x
V

G
|
G

V
|
V

A

A

G

A

A

S

G

G

Q

Q

E

E

I

I

R

S

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

V

W

W

R

K

G

G

S
x
T

A
|
A

F
|
F

G

G

V

F

R

K

G

S

R

R

T

T

E

Q

L

V

P

P

G

W

M

L

V

V

D

E

D

K

A

Y

M

M

A

N

G

K

R

E

I

I

Q

K

L

V

A

D

P

E

F

Y

V

I

T

T

H

H

T

N

L

L

P

T

L

L

E

G

K

E

I

I

N

N

E

K

A

A

F

F

D

D

L

L

M

L

H

H

N

E

G

G

Q

T

S

C

I

L

R
|
R

T

C

V

V

I

L

C47 (= C40) binding Zn(2+)
H48 (= H41) binding NAD(+)
H69 (= H62) binding Zn(2+)
E70 (= E63) binding Zn(2+)
C99 (= C92) binding Zn(2+)
C102 (= C95) binding Zn(2+)
C105 (= C98) binding Zn(2+)
C113 (= C106) binding Zn(2+)
C177 (= C169) binding Zn(2+)
GLGTVG 202:207 (≠ GLGGIG 194:199) binding NAD(+)
D226 (= D218) binding NAD(+)
K231 (= K223) binding NAD(+)
I272 (= I264) binding NAD(+)
VGV 295:297 (≠ IGV 287:289) binding NAD(+)
TAF 320:322 (≠ SAF 312:314) binding NAD(+)
R372 (= R364) binding NAD(+)

4gl4A Crystal structure of oxidized s-nitrosoglutathione reductase from arabidopsis thalina, complex with nadh
62% identity, 99% coverage: 4:367/369 of query aligns to 10:374/378 of 4gl4A

query
sites
4gl4A

R

K

A

A

V

V

A

A

F

Y

E

E

A

P

G

N

K

K

P

P

L

L

Q

V

I

I

V

E

E

D

I

V

D

Q

V

V

E

A

P

P

R

Q

A

A

G

G

E

E

V

V

L

R

V

I

K

K

I

I

T

L

H

Y

T

T

G

A

V

L

C
|
C

H
|
H

T
|
T

D

D

A

A

F
x
Y

T

T

L

W

S

S

G

G

D

K

D

D

P

P

E

E

G

G

I

L

F

F

P

P

A

C

V

I

L

L

G

G

H
|
H

E
|
E

G

A

A

A

G

G

V

I

V

V

V

E

E

S

V

V

G

G

A

E

G

G

V

V

V

T

A

E

L

V

A

Q

P

A

G

G

D

D

H

H

V

V

I

I

P

P

L

C

Y
|
Y

T

Q

A

A

E

E

C
|
C

R

R

A

E

C
|
C

K

K

F

F

C
|
C

L

K

S

S

G

G

K

K

T

T

N

N

L

L

C
|
C

Q

G

K

K

V

V

R
|
R

A

S

T

A

Q

T

G

G

K

V

G

G

-

I

L

M

M

M

P

N

D

D

A

R

S

K

S

S

R

R

F

F

S

S

Y

V

Q

N

G

G

Q

K

P

P

I

I

F

Y

H

H

Y

F

M

M

G

G

C

T

S

S

T

T

F

F

S

S

E

Q

Y

Y

T

T

V

V

N

H

E

D

I

V

S

S

L

V

A

A

K

K

I

I

N

D

P

P

A

T

A

A

N

P

H

L

E

D

Q

K

V

V

C

C

L

L

G

G

C
|
C

G

G

V

V

T

P

T
|
T

G

G

L

L

G

G

A

A

V

V

K

W

N

N

T

T

A

A

K

K

V

V

K

E

P

P

G

G

D

S

T

N

V

V

A

A

V

I

F

F

G

G

L

L

G
|
G

G

T

I
x
V

G

G

L

L

A

A

V

V

I

A

Q

E

G

G

A

A

K

K

L

T

A

A

R

G

A

A

G

S

R

R

I

I

G

G

V

I

D
|
D

T
x
I

N

D

P

S

G

K

K

K

F

Y

D

E

L

T

A

A

R

K

T

K

M

F

G

G

A

V

T

N

D

E

C

F

V

V

N

N

P

P

K

K

D

D

H

H

A

D

K

K

P

P

I

I

Q

Q

D

E

V

V

I

I

V

V

E

D

M

L

T

T

D

D

G

G

V

V

E

D

F

Y

S

S

F

F

E

E

C
|
C

I
|
I

G

G

N

N

V

V

D

S

V

V

M

M

R

R

A

A

L

L

E

E

C

C

H

H

K

K

G

G

W

W

G

G

E

T

S

S

V

V

I

I

I
x
V

G
|
G

V
|
V

A

A

G

A

A

S

G

G

Q

Q

E

E

I

I

R

S

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

V

W

W

R

K

G

G

S

T

A
|
A

F

F

G

G

V

F

R

K

G

S

R

R

T

T

E

Q

L

V

P

P

G

W

M

L

V

V

D

E

D

K

A

Y

M

M

A

N

G

K

R

E

I

I

Q

K

L

V

A

D

P

E

F

Y

V

I

T

T

H

H

T

N

L

L

P

T

L

L

E

G

K

E

I

I

N

N

E

K

A

A

F

F

D

D

L

L

M

L

H

H

N

E

G

G

Q

T

S

C

I

L

R
|
R

T

C

V

V

I

L

active site: C46 (= C40), H47 (= H41), T48 (= T42), Y51 (≠ F45), H68 (= H62), E69 (= E63), C98 (= C92), C101 (= C95), C104 (= C98), C112 (= C106), R116 (= R110), C176 (= C169), T180 (= T173), R371 (= R364)
binding 1,4-dihydronicotinamide adenine dinucleotide: H47 (= H41), Y94 (= Y88), T180 (= T173), G203 (= G196), V205 (≠ I198), D225 (= D218), I226 (≠ T219), C270 (= C263), I271 (= I264), V294 (≠ I287), G295 (= G288), V296 (= V289), A320 (= A313)
binding zinc ion: C46 (= C40), H68 (= H62), E69 (= E63), C98 (= C92), C101 (= C95), C104 (= C98), C112 (= C106), C176 (= C169)

3ukoA Crystal structure of s-nitrosoglutathione reductase from arabidopsis thaliana, complex with nadh
62% identity, 99% coverage: 4:367/369 of query aligns to 10:374/378 of 3ukoA

query
sites
3ukoA

R

K

A

A

V

V

A

A

F

Y

E

E

A

P

G

N

K

K

P

P

L

L

Q

V

I

I

V

E

E

D

I

V

D

Q

V

V

E

A

P

P

R

Q

A

A

G

G

E

E

V

V

L

R

V

I

K

K

I

I

T

L

H

Y

T

T

G

A

V

L

C
|
C

H
|
H

T
|
T

D

D

A

A

F
x
Y

T

T

L

W

S

S

G

G

D

K

D

D

P

P

E

E

G

G

I

L

F

F

P

P

A

C

V

I

L

L

G

G

H
|
H

E
|
E

G

A

A

A

G

G

V

I

V

V

V

E

E

S

V

V

G

G

A

E

G

G

V

V

V

T

A

E

L

V

A

Q

P

A

G

G

D

D

H

H

V

V

I

I

P

P

L

C

Y

Y

T

Q

A

A

E

E

C
|
C

R

R

A

E

C
|
C

K

K

F

F

C
|
C

L

K

S

S

G

G

K

K

T

T

N

N

L

L

C
|
C

Q

G

K

K

V

V

R
|
R

A

S

T

A

Q

T

G

G

K

V

G

G

-

I

L

M

M

M

P

N

D

D

A

R

S

K

S

S

R

R

F

F

S

S

Y

V

Q

N

G

G

Q

K

P

P

I

I

F

Y

H

H

Y

F

M

M

G

G

C

T

S

S

T

T

F

F

S

S

E

Q

Y

Y

T

T

V

V

N

H

E

D

I

V

S

S

L

V

A

A

K

K

I

I

N

D

P

P

A

T

A

A

N

P

H

L

E

D

Q

K

V

V

C

C

L

L

G

G

C
|
C

G

G

V

V

T

P

T
|
T

G

G

L

L

G

G

A

A

V

V

K

W

N

N

T

T

A

A

K

K

V

V

K

E

P

P

G

G

D

S

T

N

V

V

A

A

V

I

F

F

G

G

L

L

G
|
G

G

T

I
x
V

G

G

L

L

A

A

V

V

I

A

Q

E

G

G

A

A

K

K

L

T

A

A

R

G

A

A

G

S

R

R

I

I

G

G

V

I

D
|
D

T
x
I

N

D

P

S

G

K

K
|
K

F

Y

D

E

L

T

A

A

R

K

T

K

M

F

G

G

A

V

T

N

D

E

C

F

V

V

N

N

P

P

K

K

D

D

H

H

A

D

K

K

P

P

I

I

Q

Q

D

E

V

V

I

I

V

V

E

D

M

L

T

T

D

D

G

G

V

V

E

D

F

Y

S

S

F

F

E

E

C
|
C

I
|
I

G

G

N

N

V

V

D

S

V

V

M

M

R

R

A

A

L

L

E

E

C

C

H

H

K

K

G

G

W

W

G

G

E

T

S

S

V

V

I

I

I
x
V

G
|
G

V
|
V

A

A

G

A

A

S

G

G

Q

Q

E

E

I

I

R

S

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

V

W

W

R

K

G

G

S

T

A
|
A

F

F

G

G

V

F

R

K

G

S

R

R

T

T

E

Q

L

V

P

P

G

W

M

L

V

V

D

E

D

K

A

Y

M

M

A

N

G

K

R

E

I

I

Q

K

L

V

A

D

P

E

F

Y

V

I

T

T

H

H

T

N

L

L

P

T

L

L

E

G

K

E

I

I

N

N

E

K

A

A

F

F

D

D

L

L

M

L

H

H

N

E

G

G

Q

T

S

C

I

L

R
|
R

T

C

V

V

I

L

active site: C46 (= C40), H47 (= H41), T48 (= T42), Y51 (≠ F45), H68 (= H62), E69 (= E63), C98 (= C92), C101 (= C95), C104 (= C98), C112 (= C106), R116 (= R110), C176 (= C169), T180 (= T173), R371 (= R364)
binding nicotinamide-adenine-dinucleotide: H47 (= H41), T180 (= T173), G203 (= G196), V205 (≠ I198), D225 (= D218), I226 (≠ T219), K230 (= K223), C270 (= C263), I271 (= I264), V294 (≠ I287), G295 (= G288), V296 (= V289), A320 (= A313)
binding zinc ion: C46 (= C40), H68 (= H62), E69 (= E63), C98 (= C92), C101 (= C95), C104 (= C98), C112 (= C106), C176 (= C169)

P19854 Alcohol dehydrogenase class-3; Alcohol dehydrogenase 5; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Equus caballus (Horse) (see 2 papers)
64% identity, 99% coverage: 1:367/369 of query aligns to 6:372/374 of P19854

query
sites
P19854

M

I

K

K

S

C

R

K

A

A

V

V

A

A

F

W

E

E

A

A

G

G

K

K

P

P

L

V

Q

S

I

I

V

E

E

E

I

V

D

E

V

V

E

A

P

P

R

K

A

A

G

H

E

E

V

V

L

R

V

I

K

K

I

I

T

I

H

A

T

T

G

A

V

V

C

C

H

H

T

T

D

D

A

A

F

Y

T

T

L

L

S

S

G

G

D

A

D

D

P

P

E

E

G

G

I

S

F

F

P

P

A

V

V

I

L

L

G

G

H

H

E

E

G

G

A

A

G

G

V

I

V

V

V

E

E

S

V

V

G

G

A

E

G

G

V

V

V

T

A

K

L

L

A

K

P

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y

Y

T

I

A

P

E

Q

C

C

R

G

A

E

C

C

K

K

F

F

C

C

L

L

S

N

G

P

K

Q

T

T

N

N

L

L

C

C

Q

Q

K

K

V

I

R

R

A

T

T

T

Q

Q

G

G

K

K

G

G

L

L

M

M

P

P

D

D

A

G

S

T

S

S

R

R

F

F

S

T

Y

C

Q

K

G

G

Q

K

P

T

I

I

F

L

H

H

Y

Y

M

M

G

G

C

T

S

S

T

T

F

F

S

S

E

E

Y

Y

T

T

V

V

N

A

E

D

I

I

S

S

L

V

A

A

K

K

I

I

N

D

P

P

A

L

A

A

N

P

H

L

E

D

Q

K

V

V

C

C

L

L

G

G

C

C

G

G

V

V

T

S

T

T

G

G

L

Y

G

G

A

A

V

A

K

V

N

N

T

T

A

A

K

K

V

V

K

E

P

P

G

G

D

S

T

T

V

C

A

A

V

I

F

F

G

G

L

L

G

G

I

V

G

G

L

L

A

A

V

V

I

I

Q

M

G

G

A

C

K

K

L

V

A

A

R

G

A

A

G

S

R

R

I

I

G

G

V

V

D

D

T

I

N

N

P

K

G

D

K

K

F

F

D

A

L

K

A

A

R

K

T

E

M

F

G

G

A

A

T

S

D

E

C

C

V

I

N

N

P

P

K

Q

D

D

H

F

A

S

K

K

P

P

I

I

Q

Q

D

E

V

V

I

L

V

I

E

E

M

M

T

T

D

D

G

G

V

V

E

D

F

Y

S

S

F

F

E

E

C

C

I

I

G

G

N

N

V

V

D

K

V

V

M

M

R

R

A

A

L

L

E

E

C

A

C

C

H

H

K

K

G

G

W

W

G

G

E

V

S

S

V

V

I

V

G

G

V

V

A

A

G

A

A

S

G

G

Q

E

E

E

I

I

R

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

R

K

G

G

S

T

A

A

F

F

G

G

V

W

R

K

G

S

R

V

T

E

E

S

L

I

P

P

G

K

M

L

V

V

D

S

D

E

A

Y

M

M

A

S

G

K

R

K

I

I

Q

K

L

V

A

D

P

E

F

F

V

V

T

T

H

H

T

S

L

L

P

S

L

F

E

D

K

Q

I

I

N

N

E

E

A

A

F

F

D

E

L

L

M

M

H

H

N

A

G

G

Q

K

S

S

I

I

R

R

T

T

V

V

I

V

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine

P11766 Alcohol dehydrogenase class-3; Alcohol dehydrogenase 5; Alcohol dehydrogenase class chi chain; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Homo sapiens (Human) (see 3 papers)
64% identity, 99% coverage: 1:367/369 of query aligns to 6:372/374 of P11766

query
sites
P11766

M

I

K

K

S

C

R

K

A

A

V

V

A

A

F

W

E

E

A

A

G

G

K

K

P

P

L

L

Q

S

I

I

V

E

E

E

I

I

D

E

V

V

E

A

P

P

R

K

A

A

G

H

E

E

V

V

L

R

V

I

K

K

I

I

T

I

H

A

T

T

G

A

V

V

C
|
C

H

H

T

T

D

D

A

A

F

Y

T

T

L

L

S

S

G

G

D

A

D

D

P

P

E

E

G

G

I

C

F

F

P

P

A

V

V

I

L

L

G

G

H
|
H

E

E

G

G

A

A

G

G

V

I

V

V

V

E

E

S

V

V

G

G

A

E

G

G

V

V

V

T

A

K

L

L

A

K

P

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y

Y

T

I

A

P

E

Q

C
|
C

R

G

A

E

C
|
C

K

K

F

F

C
|
C

L

L

S

N

G

P

K

K

T

T

N

N

L

L

C
|
C

Q

Q

K

K

V

I

R
|
R

A

V

T

T

Q

Q

G

G

K

K

G

G

L

L

M

M

P

P

D

D

A

G

S

T

S

S

R

R

F

F

S

T

Y

C

Q

K

G

G

Q

K

P

T

I

I

F

L

H

H

Y

Y

M

M

G

G

C

T

S

S

T

T

F

F

S

S

E

E

Y

Y

T

T

V

V

N

A

E

D

I

I

S

S

L

V

A

A

K

K

I

I

N

D

P

P

A
x
L

A

A

N

P

H

L

E

D

Q

K

V

V

C

C

L

L

G

G

C
|
C

G

G

V

I

T

S

T

T

G

G

L

Y

G

G

A

A

V

A

K

V

N

N

T

T

A

A

K

K

V

L

K

E

P

P

G

G

D

S

T

V

V

C

A

A

V

V

F

F

G

G

L

L

G

G

I

V

G

G

L

L

A

A

V

V

I

I

Q

M

G

G

A

C

K

K

L

V

A

A

R

G

A

A

G

S

R

R

I

I

G

G

V

V

D

D

T

I

N

N

P

K

G

D

K

K

F

F

D

A

L

R

A

A

R

K

T

E

M

F

G

G

A

A

T

T

D

E

C

C

V

I

N

N

P

P

K

Q

D

D

H

F

A

S

K

K

P

P

I

I

Q

Q

D

E

V

V

I

L

V

I

E

E

M

M

T

T

D

D

G

G

V

V

E

D

F

Y

S

S

F

F

E

E

C

C

I

I

G

G

N

N

V

V

D

K

V

V

M

M

R

R

A

A

L

L

E

E

C

A

C

C

H

H

K

K

G

G

W

W

G

G

E

V

S

S

V

V

I

V

G

G

V

V

A

A

G

A

A

S

G

G

Q

E

E

E

I

I

R

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V
|
V

T

T

G

G

R

R

V

T

W

W

R

K

G

G

S

T

A

A

F

F

G

G

V

W

R

K

G

S

R

V

T

E

E

S

L

V

P

P

G

K

M

L

V

V

D

S

D

E

A

Y

M

M

A

S

G

K

R

K

I

I

Q

K

L

V

A

D

P

E

F

F

V

V

T

T

H

H

T

N

L

L

P

S

L

F

E

D

K

E

I

I

N

N

E

K

A

A

F

F

D

E

L

L

M

M

H

H

N

S

G

G

Q

K

S

S

I

I

R

R

T

T

V

V

I

V

C45 (= C40) binding Zn(2+)
H67 (= H62) binding Zn(2+)
C97 (= C92) binding Zn(2+)
C100 (= C95) binding Zn(2+)
C103 (= C98) binding Zn(2+)
C111 (= C106) binding Zn(2+)
R115 (= R110) Important for FDH activity and activation by fatty acids; mutation R->A,D: Loss of FDH activity and loss of activation by fatty acids.
L163 (≠ A158) to S: in dbSNP:rs28730623
C174 (= C169) binding Zn(2+)
V309 (= V304) to I: in dbSNP:rs28730628

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

2fzeA Crystal structure of the binary complex of human glutathione-dependent formaldehyde dehydrogenase with adp-ribose (see paper)
64% identity, 99% coverage: 1:367/369 of query aligns to 5:371/373 of 2fzeA

query
sites
2fzeA

M

I

K

K

S

C

R

K

A

A

V

V

A

A

F

W

E

E

A

A

G

G

K

K

P

P

L

L

Q

S

I

I

V

E

E

E

I

I

D

E

V

V

E

A

P

P

R

K

A

A

G

H

E

E

V

V

L

R

V

I

K

K

I

I

T

I

H

A

T

T

G

A

V

V

C
|
C

H
|
H

T
|
T

D

D

A

A

F
x
Y

T

T

L

L

S

S

G

G

D

A

D

D

P

P

E

E

G

G

I

C

F

F

P

P

A

V

V

I

L

L

G

G

H
|
H

E
|
E

G

G

A

A

G

G

V

I

V

V

V

E

E

S

V

V

G

G

A

E

G

G

V

V

V

T

A

K

L

L

A
x
K

P

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y

Y

T

I

A

P

E

Q

C
|
C

R

G

A

E

C
|
C

K

K

F

F

C
|
C

L

L

S

N

G

P

K

K

T

T

N

N

L

L

C
|
C

Q

Q

K

K

V

I

R
|
R

A

V

T

T

Q

Q

G

G

K

K

G

G

L

L

M

M

P

P

D

D

A

G

S

T

S

S

R

R

F

F

S

T

Y

C

Q

K

G

G

Q

K

P

T

I

I

F

L

H

H

Y

Y

M

M

G

G

C

T

S

S

T

T

F

F

S

S

E

E

Y

Y

T

T

V

V

N

A

E

D

I

I

S

S

L

V

A

A

K
|
K

I

I

N

D

P

P

A

L

A

A

N

P

H

L

E

D

Q

K

V

V

C

C

L

L

G

G

C
|
C

G

G

V

I

T

S

T
|
T

G

G

L

Y

G

G

A

A

V

A

K

V

N

N

T

T

A

A

K

K

V

L

K

E

P

P

G

G

D

S

T

V

V

C

A

A

V

V

F

F

G

G

L

L

G
|
G

G

G

I
x
V

G

G

L

L

A

A

V

V

I

I

Q

M

G

G

A

C

K

K

L

V

A

A

R

G

A

A

G

S

R

R

I

I

G

G

V

V

D
|
D

T
x
I

N

N

P

K

G

D

K

K

F

F

D

A

L

R

A

A

R

K

T

E

M

F

G

G

A

A

T

T

D

E

C

C

V

I

N

N

P

P

K

Q

D

D

H

F

A

S

K

K

P

P

I

I

Q

Q

D

E

V

V

I

L

V

I

E

E

M

M

T

T

D

D

G

G

V

V

E

D

F

Y

S

S

F

F

E

E

C

C

I
|
I

G

G

N

N

V

V

D

K

V

V

M

M

R

R

A

A

L

L

E

E

C

A

C

C

H

H

K

K

G

G

W

W

G

G

E

V

S

S

V

V

I

V

G
|
G

V

V

A

A

G

A

A

S

G

G

Q

E

E

E

I

I

R

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

R

K

G

G

S

T

A

A

F

F

G

G

V

W

R

K

G

S

R

V

T

E

E

S

L

V

P

P

G

K

M

L

V

V

D

S

D

E

A

Y

M

M

A

S

G

K

R

K

I

I

Q

K

L

V

A

D

P

E

F

F

V

V

T

T

H

H

T

N

L

L

P

S

L

F

E

D

K

E

I

I

N

N

E

K

A

A

F

F

D

E

L

L

M

M

H

H

N

S

G

G

Q

K

S

S

I

I

R
|
R

T

T

V

V

I

V

active site: C44 (= C40), H45 (= H41), T46 (= T42), Y49 (≠ F45), H66 (= H62), E67 (= E63), C96 (= C92), C99 (= C95), C102 (= C98), C110 (= C106), R114 (= R110), C173 (= C169), T177 (= T173), R368 (= R364)
binding adenosine-5-diphosphoribose: G200 (= G196), V202 (≠ I198), D222 (= D218), I223 (≠ T219), I268 (= I264), G292 (= G288)
binding phosphate ion: K83 (≠ A79), K158 (= K154)
binding zinc ion: H66 (= H62), C96 (= C92), C99 (= C95), C102 (= C98), C110 (= C106), C173 (= C169)

1mc5A Ternary complex of human glutathione-dependent formaldehyde dehydrogenase with s-(hydroxymethyl)glutathione and nadh (see paper)
64% identity, 99% coverage: 1:367/369 of query aligns to 5:371/373 of 1mc5A

query
sites
1mc5A

M

I

K

K

S

C

R

K

A

A

V

V

A

A

F

W

E

E

A

A

G

G

K

K

P

P

L

L

Q

S

I

I

V

E

E

E

I

I

D

E

V

V

E

A

P

P

R

K

A

A

G

H

E

E

V

V

L

R

V

I

K

K

I

I

T

I

H

A

T

T

G

A

V

V

C
|
C

H
|
H

T
|
T

D

D

A

A

F
x
Y

T

T

L

L

S

S

G

G

D

A

D

D

P
|
P

E
|
E

G

G

I

C

F

F

P

P

A

V

V

I

L

L

G

G

H
|
H

E
|
E

G

G

A

A

G

G

V

I

V

V

V

E

E

S

V

V

G

G

A

E

G

G

V

V

V

T

A

K

L

L

A

K

P

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y
|
Y

T

I

A

P

E

Q

C
|
C

R

G

A

E

C
|
C

K

K

F

F

C
|
C

L

L

S

N

G

P

K

K

T

T

N

N

L

L

C
|
C

Q

Q

K

K

V

I

R
|
R

A

V

T

T

Q

Q

G

G

K

K

G

G

L

L

M

M

P

P

D

D

A

G

S

T

S

S

R

R

F

F

S

T

Y

C

Q

K

G

G

Q

K

P

T

I

I

F

L

H

H

Y

Y

M

M

G

G

C

T

S

S

T

T

F

F

S

S

E

E

Y

Y

T

T

V

V

N

A

E

D

I

I

S

S

L

V

A

A

K

K

I

I

N

D

P

P

A

L

A

A

N

P

H

L

E

D

Q

K

V

V

C

C

L

L

G

G

C
|
C

G

G

V

I

T

S

T
|
T

G

G

L

Y

G

G

A

A

V

A

K

V

N

N

T

T

A

A

K

K

V

L

K

E

P

P

G

G

D

S

T

V

V

C

A

A

V

V

F

F

G
|
G

L

L

G

G

G
|
G

I
x
V

G

G

L

L

A

A

V

V

I

I

Q

M

G

G

A

C

K

K

L

V

A

A

R

G

A

A

G

S

R

R

I

I

G

G

V

V

D
|
D

T
x
I

N

N

P

K

G

D

K
|
K

F

F

D

A

L

R

A

A

R

K

T

E

M

F

G

G

A

A

T

T

D

E

C

C

V

I

N

N

P

P

K

Q

D

D

H

F

A

S

K

K

P

P

I

I

Q

Q

D

E

V

V

I

L

V

I

E

E

M

M

T

T

D

D

G

G

V

V

E

D

F

Y

S

S

F

F

E

E

C
|
C

I
|
I

G

G

N

N

V

V

D

K

V

V

M

M

R

R

A

A

L

L

E

E

C

A

C

C

H

H

K

K

G

G

W

W

G

G

E

V

S

S

V

V

I

V

I
x
V

G
|
G

V
|
V

A

A

G

A

A

S

G

G

Q

E

E

E

I

I

R

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

R

K

G

G

S

T

A
|
A

F

F

G

G

V

W

R

K

G

S

R

V

T

E

E

S

L

V

P

P

G

K

M

L

V

V

D

S

D

E

A

Y

M

M

A

S

G

K

R

K

I

I

Q

K

L

V

A

D

P

E

F

F

V

V

T

T

H

H

T

N

L

L

P

S

L

F

E

D

K

E

I

I

N

N

E

K

A

A

F

F

D

E

L

L

M

M

H

H

N

S

G

G

Q

K

S

S

I

I

R
|
R

T

T

V

V

I

V

active site: C44 (= C40), H45 (= H41), T46 (= T42), Y49 (≠ F45), H66 (= H62), E67 (= E63), C96 (= C92), C99 (= C95), C102 (= C98), C110 (= C106), R114 (= R110), C173 (= C169), T177 (= T173), R368 (= R364)
binding 2-amino-4-[1-carboxymethyl-carbamoyl)-2-hydroxymethylsulfanyl-ethylcarbamoyl]-butyric acid: T46 (= T42), P56 (= P52), E57 (= E53), H66 (= H62), Y92 (= Y88), R114 (= R110), C173 (= C169), V293 (= V289)
binding nicotinamide-adenine-dinucleotide: C44 (= C40), H45 (= H41), T46 (= T42), C173 (= C169), T177 (= T173), G198 (= G194), G201 (= G197), V202 (≠ I198), D222 (= D218), I223 (≠ T219), K227 (= K223), C267 (= C263), I268 (= I264), V291 (≠ I287), G292 (= G288), V293 (= V289), A317 (= A313), R368 (= R364)
binding zinc ion: C44 (= C40), H66 (= H62), C96 (= C92), C99 (= C95), C102 (= C98), C110 (= C106), C173 (= C169)

1ma0A Ternary complex of human glutathione-dependent formaldehyde dehydrogenase with NAD+ and dodecanoic acid (see paper)
64% identity, 99% coverage: 1:367/369 of query aligns to 5:371/373 of 1ma0A

query
sites
1ma0A

M

I

K

K

S

C

R

K

A

A

V

V

A

A

F

W

E

E

A

A

G

G

K

K

P

P

L

L

Q

S

I

I

V

E

E

E

I

I

D

E

V

V

E

A

P

P

R

K

A

A

G

H

E

E

V

V

L

R

V

I

K

K

I

I

T

I

H

A

T

T

G

A

V

V

C
|
C

H
|
H

T
|
T

D

D

A

A

F
x
Y

T

T

L

L

S

S

G

G

D

A

D

D

P

P

E

E

G

G

I

C

F

F

P

P

A

V

V

I

L

L

G

G

H
|
H

E
|
E

G

G

A

A

G

G

V

I

V

V

V

E

E

S

V

V

G

G

A

E

G

G

V

V

V

T

A

K

L

L

A

K

P

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y

Y

T

I

A

P

E

Q

C
|
C

R

G

A

E

C
|
C

K

K

F

F

C
|
C

L

L

S

N

G

P

K

K

T

T

N

N

L

L

C
|
C

Q

Q

K

K

V

I

R
|
R

A

V

T

T

Q

Q

G

G

K

K

G

G

L

L

M

M

P

P

D

D

A

G

S

T

S

S

R

R

F

F

S

T

Y

C

Q

K

G

G

Q

K

P

T

I

I

F

L

H

H

Y

Y

M

M

G

G

C

T

S

S

T

T

F

F

S

S

E

E

Y

Y

T

T

V

V

N

A

E

D

I

I

S

S

L

V

A

A

K

K

I

I

N

D

P

P

A

L

A

A

N

P

H

L

E

D

Q

K

V

V

C

C

L

L

G

G

C
|
C

G

G

V

I

T

S

T
|
T

G

G

L

Y

G

G

A

A

V

A

K

V

N

N

T

T

A

A

K

K

V

L

K

E

P

P

G

G

D

S

T

V

V

C

A

A

V

V

F

F

G

G

L

L

G
|
G

G

G

I
x
V

G

G

L

L

A

A

V

V

I

I

Q

M

G

G

A

C

K

K

L

V

A

A

R

G

A

A

G

S

R

R

I

I

G

G

V

V

D
|
D

T
x
I

N

N

P

K

G

D

K

K

F

F

D

A

L

R

A

A

R

K

T

E

M

F

G

G

A

A

T

T

D

E

C

C

V

I

N

N

P

P

K

Q

D

D

H

F

A

S

K

K

P

P

I

I

Q

Q

D

E

V

V

I

L

V

I

E

E

M

M

T

T

D

D

G

G

V

V

E

D

F

Y

S

S

F

F

E

E

C
|
C

I
|
I

G

G

N

N

V

V

D

K

V

V

M

M

R

R

A

A

L

L

E

E

C

A

C

C

H

H

K

K

G

G

W

W

G

G

E

V

S

S

V

V

I

V

I
x
V

G
|
G

V
|
V

A

A

G

A

A

S

G

G

Q

E

E

E

I

I

R

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

R

K

G

G

S

T

A
|
A

F
|
F

G

G

V

W

R

K

G

S

R

V

T

E

E

S

L

V

P

P

G

K

M

L

V

V

D

S

D

E

A

Y

M

M

A

S

G

K

R

K

I

I

Q

K

L

V

A

D

P

E

F

F

V

V

T

T

H

H

T

N

L

L

P

S

L

F

E

D

K

E

I

I

N

N

E

K

A

A

F

F

D

E

L

L

M

M

H

H

N

S

G

G

Q

K

S

S

I

I

R
|
R

T

T

V

V

I

V

active site: C44 (= C40), H45 (= H41), T46 (= T42), Y49 (≠ F45), H66 (= H62), E67 (= E63), C96 (= C92), C99 (= C95), C102 (= C98), C110 (= C106), R114 (= R110), C173 (= C169), T177 (= T173), R368 (= R364)
binding nicotinamide-adenine-dinucleotide: H45 (= H41), T177 (= T173), G200 (= G196), V202 (≠ I198), D222 (= D218), I223 (≠ T219), C267 (= C263), I268 (= I264), V291 (≠ I287), G292 (= G288), V293 (= V289), A317 (= A313), F318 (= F314)
binding zinc ion: C44 (= C40), H66 (= H62), E67 (= E63), C96 (= C92), C99 (= C95), C102 (= C98), C110 (= C106), C173 (= C169)

1m6wA Binary complex of human glutathione-dependent formaldehyde dehydrogenase and 12-hydroxydodecanoic acid (see paper)
64% identity, 99% coverage: 1:367/369 of query aligns to 5:371/373 of 1m6wA

query
sites
1m6wA

M

I

K

K

S

C

R

K

A

A

V

V

A

A

F

W

E

E

A

A

G

G

K

K

P

P

L

L

Q

S

I

I

V

E

E

E

I

I

D

E

V

V

E

A

P

P

R

K

A

A

G

H

E

E

V

V

L

R

V

I

K

K

I

I

T

I

H

A

T

T

G

A

V

V

C
|
C

H
|
H

T
|
T

D

D

A

A

F
x
Y

T

T

L

L

S

S

G

G

D

A

D

D

P

P

E

E

G

G

I

C

F

F

P

P

A

V

V

I

L

L

G

G

H
|
H

E
|
E

G

G

A

A

G

G

V

I

V

V

V

E

E

S

V

V

G

G

A

E

G

G

V

V

V

T

A

K

L

L

A

K

P

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y
|
Y

T

I

A

P

E

Q

C
|
C

R

G

A

E

C
|
C

K

K

F

F

C
|
C

L

L

S

N

G

P

K

K

T

T

N

N

L

L

C
|
C

Q
|
Q

K

K

V

I

R
|
R

A

V

T

T

Q

Q

G

G

K

K

G

G

L

L

M

M

P

P

D

D

A

G

S

T

S

S

R

R

F

F

S

T

Y

C

Q

K

G

G

Q

K

P

T

I

I

F

L

H

H

Y

Y

M

M

G

G

C

T

S

S

T

T

F

F

S

S

E

E

Y

Y

T

T

V

V

N

A

E

D

I

I

S

S

L

V

A

A

K

K

I

I

N

D

P

P

A

L

A

A

N

P

H

L

E

D

Q

K

V

V

C

C

L

L

G

G

C
|
C

G

G

V

I

T

S

T
|
T

G

G

L

Y

G

G

A

A

V

A

K

V

N

N

T

T

A

A

K

K

V

L

K

E

P

P

G

G

D

S

T

V

V

C

A

A

V

V

F

F

G

G

L

L

G

G

I

V

G

G

L

L

A

A

V

V

I

I

Q

M

G

G

A

C

K

K

L

V

A

A

R

G

A

A

G

S

R

R

I

I

G

G

V

V

D

D

T

I

N

N

P

K

G

D

K

K

F

F

D

A

L

R

A

A

R

K

T

E

M

F

G

G

A

A

T

T

D

E

C

C

V

I

N

N

P

P

K

Q

D

D

H

F

A

S

K

K

P

P

I

I

Q

Q

D

E

V

V

I

L

V

I

E

E

M

M

T

T

D

D

G

G

V

V

E

D

F

Y

S

S

F

F

E

E

C

C

I

I

G

G

N

N

V

V

D

K

V

V

M

M

R

R

A

A

L

L

E

E

C

A

C

C

H

H

K

K

G

G

W

W

G

G

E

V

S

S

V

V

I

V

G

G

V

V

A

A

G

A

A

S

G

G

Q

E

E

E

I

I

R

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

R

K

G

G

S

T

A

A

F

F

G

G

V

W

R

K

G

S

R

V

T

E

E

S

L

V

P

P

G

K

M

L

V

V

D

S

D

E

A

Y

M

M

A

S

G

K

R

K

I

I

Q

K

L

V

A

D

P

E

F

F

V

V

T

T

H

H

T

N

L

L

P

S

L

F

E

D

K

E

I

I

N

N

E

K

A

A

F

F

D

E

L

L

M

M

H

H

N

S

G

G

Q

K

S

S

I

I

R
|
R

T

T

V

V

I

V

active site: C44 (= C40), H45 (= H41), T46 (= T42), Y49 (≠ F45), H66 (= H62), E67 (= E63), C96 (= C92), C99 (= C95), C102 (= C98), C110 (= C106), R114 (= R110), C173 (= C169), T177 (= T173), R368 (= R364)
binding 12-hydroxydodecanoic acid: T46 (= T42), H66 (= H62), Y92 (= Y88), Q111 (= Q107), R114 (= R110), C173 (= C169)
binding zinc ion: C44 (= C40), H66 (= H62), C96 (= C92), C99 (= C95), C102 (= C98), C110 (= C106), C173 (= C169)

3qj5A S-nitrosoglutathione reductase (gsnor) in complex with n6022 (see paper)
64% identity, 99% coverage: 1:367/369 of query aligns to 4:370/372 of 3qj5A

query
sites
3qj5A

M

I

K

K

S

C

R

K

A

A

V

V

A

A

F

W

E

E

A

A

G

G

K

K

P

P

L

L

Q

S

I

I

V

E

E

E

I

I

D

E

V

V

E

A

P

P

R

K

A

A

G

H

E

E

V

V

L

R

V

I

K

K

I

I

T

I

H

A

T

T

G

A

V

V

C
|
C

H
|
H

T
|
T

D

D

A

A

F
x
Y

T

T

L

L

S

S

G

G

D

A

D

D

P

P

E

E

G

G

I

C

F

F

P

P

A

V

V

I

L

L

G

G

H
|
H

E
|
E

G

G

A

A

G

G

V

I

V

V

V

E

E

S

V

V

G

G

A

E

G

G

V

V

V

T

A

K

L

L

A

K

P

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y
|
Y

T
x
I

A

P

E

Q

C
|
C

R

G

A

E

C
|
C

K

K

F

F

C
|
C

L

L

S

N

G

P

K

K

T

T

N

N

L

L

C
|
C

Q
|
Q

K

K

V

I

R
|
R

A

V

T

T

Q
|
Q

G

G

K

K

G

G

L

L

M

M

P

P

D

D

A

G

S

T

S

S

R

R

F

F

S

T

Y

C

Q

K

G

G

Q

K

P

T

I

I

F

L

H

H

Y

Y

M

M

G

G

C

T

S

S

T

T

F

F

S

S

E

E

Y

Y

T

T

V

V

N

A

E

D

I

I

S

S

L

V

A

A

K

K

I

I

N

D

P

P

A

L

A

A

N

P

H

L

E

D

Q

K

V

V

C

C

L

L

G

G

C
|
C

G

G

V

I

T

S

T
|
T

G

G

L

Y

G

G

A

A

V

A

K

V

N

N

T

T

A

A

K

K

V

L

K

E

P

P

G

G

D

S

T

V

V

C

A

A

V

V

F

F

G

G

L

L

G
|
G

G

G

I
x
V

G

G

L

L

A

A

V

V

I

I

Q

M

G

G

A

C

K

K

L

V

A

A

R

G

A

A

G

S

R

R

I

I

G

G

V

V

D
|
D

T
x
I

N

N

P

K

G

D

K

K

F

F

D

A

L

R

A

A

R

K

T

E

M

F

G

G

A

A

T

T

D

E

C

C

V

I

N

N

P

P

K

Q

D

D

H

F

A

S

K

K

P

P

I

I

Q

Q

D

E

V

V

I

L

V

I

E

E

M

M

T

T

D

D

G

G

V

V

E

D

F

Y

S

S

F

F

E

E

C
|
C

I
|
I

G

G

N

N

V

V

D

K

V

V

M

M

R

R

A

A

L

L

E

E

C

A

C

C

H

H

K

K

G

G

W

W

G

G

E

V

S

S

V

V

I

V

I
x
V

G
|
G

V
|
V

A

A

G
x
A

A

S

G

G

Q

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active site: C43 (= C40), H44 (= H41), T45 (= T42), Y48 (≠ F45), H65 (= H62), E66 (= E63), C95 (= C92), C98 (= C95), C101 (= C98), C109 (= C106), R113 (= R110), C172 (= C169), T176 (= T173), R367 (= R364)
binding 3-{1-(4-carbamoyl-2-methylphenyl)-5-[4-(1H-imidazol-1-yl)phenyl]-1H-pyrrol-2-yl}propanoic acid: C43 (= C40), T45 (= T42), H65 (= H62), Y91 (= Y88), I92 (≠ T89), Q110 (= Q107), Q116 (= Q113), C172 (= C169)
binding pentane-1,5-diamine: H44 (= H41), Y48 (≠ F45), V292 (= V289), A294 (≠ G291)
binding nicotinamide-adenine-dinucleotide: C172 (= C169), T176 (= T173), G199 (= G196), V201 (≠ I198), D221 (= D218), I222 (≠ T219), C266 (= C263), I267 (= I264), V290 (≠ I287), G291 (= G288), V292 (= V289), A316 (= A313), F317 (= F314)
binding zinc ion: C43 (= C40), H65 (= H62), C95 (= C92), C98 (= C95), C101 (= C98), C109 (= C106), C172 (= C169)

8gv3A The cryo-em structure of gsnor with nyy001
64% identity, 99% coverage: 1:367/369 of query aligns to 4:370/373 of 8gv3A

query
sites
8gv3A

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binding nicotinamide-adenine-dinucleotide: C172 (= C169), V201 (≠ I198), D221 (= D218), I222 (≠ T219), C266 (= C263), I267 (= I264), V290 (≠ I287), G291 (= G288)
binding (4P)-4-{2-[4-(1H-imidazol-1-yl)phenyl]-5-[3-oxo-3-(2-oxo-1,3-thiazolidin-3-yl)propyl]-1H-pyrrol-1-yl}-3-methylbenzamide: T45 (= T42), Y91 (= Y88), I92 (≠ T89), P93 (≠ A90), L108 (= L105), C109 (= C106), M139 (= M136), C172 (= C169)
binding zinc ion: C43 (= C40), H65 (= H62), C101 (= C98), C109 (= C106), C172 (= C169)

P12711 Alcohol dehydrogenase class-3; Alcohol dehydrogenase 2; Alcohol dehydrogenase 5; Alcohol dehydrogenase B2; ADH-B2; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Rattus norvegicus (Rat) (see 2 papers)
64% identity, 100% coverage: 1:368/369 of query aligns to 6:373/374 of P12711

query
sites
P12711

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Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine

P80572 Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Pisum sativum (Garden pea) (Lathyrus oleraceus) (see 2 papers)
61% identity, 99% coverage: 4:367/369 of query aligns to 10:374/378 of P80572

query
sites
P80572

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Sites not aligning to the query:

1 modified: N-acetylalanine

Query Sequence

>WP_011811717.1 NCBI__GCF_000015565.1:WP_011811717.1
MKSRAAVAFEAGKPLQIVEIDVEPPRAGEVLVKITHTGVCHTDAFTLSGDDPEGIFPAVL
GHEGAGVVVEVGAGVVALAPGDHVIPLYTAECRACKFCLSGKTNLCQKVRATQGKGLMPD
ASSRFSYQGQPIFHYMGCSTFSEYTVVNEISLAKINPAANHEQVCLLGCGVTTGLGAVKN
TAKVKPGDTVAVFGLGGIGLAVIQGAKLARAGRIIGVDTNPGKFDLARTMGATDCVNPKD
HAKPIQDVIVEMTDGGVEFSFECIGNVDVMRAALECCHKGWGESVIIGVAGAGQEIRTRP
FQLVTGRVWRGSAFGGVRGRTELPGMVDDAMAGRIQLAPFVTHTLPLEKINEAFDLMHNG
QSIRTVIHY

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory