SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011812370.1 NCBI__GCF_000015565.1:WP_011812370.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 11 hits to proteins with known functional sites (download)

P13804 Electron transfer flavoprotein subunit alpha, mitochondrial; Alpha-ETF from Homo sapiens (Human) (see 6 papers)
57% identity, 100% coverage: 2:310/310 of query aligns to 21:330/333 of P13804

query
sites
P13804

A
x
S

A
x
T

L
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L

V
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V

I
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I

A
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A

E
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E

H
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H

D
x
A

H
x
N

A
x
D

S
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S

I
x
L

K
x
A

P
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P

A
x
I

T
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T

L
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L

N
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N

T
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T

V
x
I

T
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T

A
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A

I
x
T

A
x
R

C
x
L

G
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G

E
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E

V
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V

H
x
S

V
x
C

L
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L

V
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V

A
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A

G
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G

A
x
T

N
x
K

A
x
C

A
x
D

E
x
K

A
x
V

G
x
A

K
x
Q

A
x
D

A
x
L

A
x
C

Q
x
K

I
x
V

A
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A

G
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G

V
x
I

A
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A

K
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K

V
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V

I
x
L

V
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V

A
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A

D
x
Q

S
x
H

P
x
D

S
x
V

L
x
Y

A
x
K

H
x
G

N
x
L

L
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L

A
x
P

E
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E

N
x
E

V
x
L

A
x
T

A
x
P

Q
x
L

V
x
I

L
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L

T
x
A

I
x
T

A
x
Q

G
x
K

-
x
Q

-
x
F

N
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N

Y
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Y

S
x
T

H
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H

I
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I

L
x
C

F
x
A

P
x
G

S
x
A

T
x
S

A
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A

G
x
F

G
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G

K
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K

N
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N

V
x
L

A
x
L

P
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P

R
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R

V
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V

A
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A

K
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K

L
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L

D
x
E

V
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V

A
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A

Q
x
P

I
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I

S
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S

D
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D

I
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I

T
x
I

K
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A

V
x
I

I
x
K

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S

A
x
P

D
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D

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T

F
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F

E
x
V

R
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R

P
x
T

I
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I

Y
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Y

A
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A

G
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G

N
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N

A
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A

I
x
L

A
x
C

T
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T

V
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V

Q
x
K

S
x
C

A
x
D

D
x
E

A
x
K

T
x
V

K
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K

V
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V

I
x
F

T
x
S

V
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V

R
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R

T
x
G

T
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T

G
x
S

F
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F

D
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D

A
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A

T
x
A

A
x
T

T
x
S

G
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G

S
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S

A
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A

T
x
S

V
x
S

E
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E

N
x
K

A
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A

E
x
S

G
x
S

V
x
T

A
x
S

D
x
P

S
x
V

G
x
E

K
x
I

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S

A
x
E

F
x
W

V
x
L

G
x
D

A
x
Q

E
x
K

I
x
L

A
x
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K
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K

N
x
S

D
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D

R
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R

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P

E
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E

L
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L

T
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T

A
x
G

A
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A

K
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K

I
x
V

V
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V

S
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S

G
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G

R
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R

A
x
G

L
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L

G
x
K

S
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S

A
x
G

E
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E

K
x
N

F
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F

T

-

E
x
K

V
x
L

M
x
L

T
x
Y

P
x
D

L
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L

A
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A

D
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D

K
x
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L
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L

N
x
H

A
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A

G
x
A

L
x
V

G
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G

A
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A

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S

R
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R

A
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A

V
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V

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D

A
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A

G
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G

Y
x
F

A
x
V

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P

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N

D
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D

W
x
M

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Q

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V

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G

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Q

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T

G
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G

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K

I
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I

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V

A
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A

P
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P

Q
x
E

L
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L

Y
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Y

I
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I

A
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A

A
x
V

G
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G

I
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I

S
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S

G
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G

A
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A

I
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I

Q
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Q

H
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H

L
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L

A
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A

G
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G

M
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M

K
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K

D
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D

S
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S

K
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K

V
x
T

I
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I

V
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V

A
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A

I
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I

N
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N

K
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K

D
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D

E
x
P

E
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E

A
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A

P
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P

I
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I

F
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F

S
x
Q

V
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V

A
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A

D
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D

Y
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Y

G
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G

L
x
I

V
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V

A
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A

D
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D

L
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L

F
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F

V
x
K

A
x
V

V
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V

P
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P

E
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E

L
x
M

T
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T

T
x
E

T
x
I

I
x
L

G116 (= G95) to R: in GA2A; impaired protein stability and loss of electron transfer activity; dbSNP:rs119458971
T171 (= T150) to I: decreased protein stability; dbSNP:rs1801591
R223 (= R202) binding FAD
S248 (= S228) binding FAD
R249 (= R229) mutation to A: Loss of electron transfer activity.
VGQT 263:266 (= VGQT 243:246) binding FAD
T266 (= T246) to M: in GA2A; decreased electron transfer activity; dbSNP:rs119458970
SGAIQH 281:286 (= SGAIQH 261:266) binding FAD
N300 (= N280) binding FAD
DL 318:319 (= DL 298:299) binding FAD

Sites not aligning to the query:

20:204 Domain I
205:333 Domain II

2a1uA Crystal structure of the human etf e165betaa mutant (see paper)
57% identity, 100% coverage: 2:310/310 of query aligns to 3:312/315 of 2a1uA

query
sites
2a1uA

A

S

A

T

L

L

V

V

I

I

A

A

E

E

H

H

D

A

H

N

A

D

S

S

I

L

K

A

P

P

A

I

T

T

L

L

N

N

T

T

V

I

T

T

A

A

I

T

A

R

C

L

G

G

E

E

V

V

H

S

V

C

L

L

V

V

A

A

G

G

A

T

N

K

A

C

A

D

E

K

A

V

G

A

K

Q

A

D

A

L

A

C

Q

K

I

V

A

A

G

G

V

I

A

A

K

K

V

V

I

L

V

V

A

A

D

Q

S

H

P

D

S

V

L

Y

A

K

H

G

N

L

L

L

A

P

E

E

N

E

V

L

A

T

A

P

Q

L

V

I

L

L

T

A

I

T

A

Q

G

K

-

Q

-

F

N

N

Y

Y

S

T

H

H

I

I

L

C

F

A

P

G

S

A

T

S

A

A

G

F

G

G

K

K

N

N

V

L

A

L

P

P

R

R

V

V

A

A

K

K

L

L

D

E

V

V

A

A

Q

P

I

I

S

S

D

D

I

I

T

I

K

A

V

I

I

K

S

S

A

P

D

D

T

T

F

F

E

V

R

R

P

T

I

I

Y

Y

A

A

G

G

N

N

A

A

I

L

A

C

T

T

V

V

Q

K

S

C

A

D

D

E

A

K

T

V

K

K

V

V

I

F

T

S

V

V

R

R

T

G

T

T

G

S

F

F

D

D

A

A

T

A

A

T

T

S

G

G

S

S

A

A

T

S

V

S

E

E

N

K

A

A

E

S

G

S

V

T

A

S

D

P

S

V

G

E

K

I

S

S

A

E

F

W

V

L

G

D

A

Q

E

K

I

L

A

T

K

K

N

S

D

D

R

R

P

P

E

E

L

L

T

T

A

G

A

A

K

K

I

V

V

V

S

S

G

G

G
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G

R
|
R

A

G

L

L

G

K

S

S

A

G

E

E

K

N

F

F

T

-

E

K

V

L

M

L

T

Y

P

D

L

L

A

A

D

D

K

Q

L

L

N

H

A

A

G

A

L

V

G

G

A

A

S
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S

R
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R

A
|
A

A

A

V

V

D

D

A

A

G

G

Y

F

A

V

P

P

N

N

D

D

W

M

Q
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Q

V
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V

G
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G

Q

Q

T
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T

G

G

K

K

I

I

V

V

A

A

P

P

Q

E

L

L

Y

Y

I

I

A

A

A

V

G
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G

I
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I

S
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S

G

G

A
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A

I

I

Q
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Q

H
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H

L

L

A

A

G

G

M

M

K

K

D

D

S

S

K

K

V

T

I

I

V

V

A

A

I

I

N
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N

K
|
K

D

D

E

P

E

E

A

A

P

P

I

I

F

F

S

Q

V

V

A

A

D

D

Y

Y

G

G

L

I

V

V

A

A

D
|
D

L
|
L

F

F

V

K

A

V

V

V

P

P

E

E

L

M

T

T

T

E

T

I

I

L

binding flavin-adenine dinucleotide: G204 (= G201), R205 (= R202), S230 (= S228), R231 (= R229), A232 (= A230), Q244 (= Q242), V245 (= V243), G246 (= G244), T248 (= T246), G261 (= G259), I262 (= I260), S263 (= S261), A265 (= A263), Q267 (= Q265), H268 (= H266), N282 (= N280), K283 (= K281), D300 (= D298), L301 (= L299)

1efpA Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
65% identity, 90% coverage: 30:307/310 of query aligns to 29:304/307 of 1efpA

query
sites
1efpA

G

G

E

D

V

V

H

T

V

V

L

L

V

C

A

A

G

G

A

A

N

S

A

A

A

K

E

A

A

A

G

A

K

E

A

E

A

A

Q

K

I

I

A

A

G

G

V

V

A

A

K

K

V

V

I

L

V

V

A

A

D

E

S

D

P

A

S

L

L

Y

A

G

H

H

N

R

L

L

A

A

E

E

N

P

V

T

A

A

Q

L

V

I

L

V

T

G

I

L

A

A

G

G

N

D

Y

Y

S

S

H

H

I

I

L

A

F

A

P

P

S

A

T

T

A

T

G

D

G

A

K

K

N

N

V

V

A

M

P

P

R

R

V

V

A

A

K

L

L

L

D

D

V

V

A

M

Q

V

I

L

S

S

D

D

I

V

T

S

K

A

V

I

I

L

S

D

A

A

D

D

T

T

F

F

E

E

R

R

P

P

I

I

Y

Y

A

A

G

G

N

N

A

A

I

I

A

Q

T

V

V

V

Q

K

S

S

A

K

D

D

A

A

T

K

K

K

V

V

I

F

T

T

V

I

R

R

T

T

T

A

G

S

F

F

D

D

A

A

T

-

A

G

T

E

G

G

S

T

A

A

T

P

V

V

E

T

N

E

A

T

E

A

G

A

V

A

A

A

D

D

S

P

G

G

K

L

S

S

A

S

F

W

V

V

G

A

A

D

E

E

I

V

A

A

K

E

N

S

D

D

R

R

P

P

E

E

L

L

T

T

A

S

A

A

K

R

I

R

I

V

V

V

S

S

G

G

G
|
G

R
|
R

A
x
G

L

L

G

G

S

S

A

K

E

E

K

S

F

F

T

A

E

-

V

I

M

I

T

E

P

E

L

L

A

A

D

D

K

K

L

L

N

G

A

A

G

A

L

V

G

G

A

A

S
|
S

R
|
R

A
|
A

A

A

V

V

D

D

A

S

G

G

Y

Y

A

A

P

P

N

N

D

D

W

W

Q
|
Q

V
|
V

G
|
G

Q

Q

T
|
T

G

G

K

K

I

V

V

V

A

A

P

P

Q

E

L

L

Y

Y

I

V

A

A

A

V

G
|
G

I
|
I

S
|
S

G

G

A
|
A

I

I

Q
|
Q

H
|
H

L

L

A

A

G

G

M

M

K

K

D

D

S

S

K

K

V

V

I

I

V

V

A

A

I

I

N
|
N

K
|
K

D

D

E

E

A

A

P

P

I

I

F

F

S

Q

V

I

A

A

D

D

Y

Y

G

G

L

L

V

V

A

G

D
|
D

L
|
L

F

F

V

S

A

V

V

V

P

P

E

E

L

L

T

T

binding flavin-adenine dinucleotide: G199 (= G201), R200 (= R202), G201 (≠ A203), S225 (= S228), R226 (= R229), A227 (= A230), Q239 (= Q242), V240 (= V243), G241 (= G244), T243 (= T246), G256 (= G259), I257 (= I260), S258 (= S261), A260 (= A263), Q262 (= Q265), H263 (= H266), N277 (= N280), K278 (= K281), D295 (= D298), L296 (= L299)

6fahE Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
38% identity, 97% coverage: 5:304/310 of query aligns to 70:382/393 of 6fahE

query
sites
6fahE

V

V

I

F

A

A

E

E

H

Q

D

R

H

E

A

G

S

K

I

I

K

M

P

P

A

V

T

V

L

F

N

E

T

L

V

L

-

G

-

E

-

G

-

K

T

K

A

L

A

A

I

N

A

E

C

I

G

G

G

T

E

E

V

L

H

C

V

A

L

I

V

L

A

C

G

G

A

S

N

N

A

V

A

A

E

E

A

L

G

T

K

D

A

E

A

L

A

F

Q

A

I

Y

A

-

G

G

V

A

A

D

K

K

V

V

I

Y

V

L

A

A

D

D

S

A

P

P

S

E

L

L

A

E

H

K

N

Y

L

T

A

T

E

D

N

G

V

Y

A

S

A

K

Q

I

V

I

L

N

T

E

I

A

A

I

G

G

N

L

Y

Y

S

K

H

P

-

E

-

I

I

V

L

L

F

Y

P

G

S

A

T

T

A

H

G

I

G

G

K

R

N

D

V

L

A

A

P

P

R

C

V

L

A

A

A

V

K

K

L

V

D

N

V

T

A

G

Q
x
L

I

T

S

A

D

D

I

C

T

T

K

K

V

L

-

E

I

I

S

D

A

P

D

D

T

D

F

K

E

K

-

I

-

R

-

Q

-

T

R
|
R

P

P

I

A

Y

F

A

G

G

G

N

N

A

L

I

M

A

A

T

T

-

I

V

V

Q

C

S

P

A

G

D

S

A

R

T

P

K

Q

V

M

I

S

T

T

V

V

R

R

T

P

T

G

G

V

F

M

D

D

-

K

A

A

T

Y

A

D

T

P

G

S

G

Q

S

K

A

G

T

E

V

V

E

I

N

K

A

L

E

D

G

A

V

T

A

F

D

N

S

E

G

G

K

D

S

I

A

R

F

T

V

K

G

V

A

L

E

E

I

I

A

V

K

K

N

T

-

T

D

D

R

N

P

I

E

S

L

I

T

S

A

D

A

A

K

D

I

F

I

I

V

V

S

S

G

G

R
x
M

A
x
G

L

L

G

G

S

K

A

P

E

E

K

G

F

F

T

-

E

E

V

L

M

L

T

K

P

Q

L

L

A

A

D

D

K

K

L

L

N

G

A

G

G

T

L

V

G

A

A

T

S

S

R
|
R

A
|
A

A

C

V

V

D

D

A

A

G

G

Y

W

A

A

P

D

N

H

D

A

W

Q

Q
|
Q

V
|
V

G
|
G

Q
|
Q

T
|
T

G

G

K

T

I

T

V

V

A

K

P

P

Q

Q

L

I

Y

Y

I

F

A

A

A

C

G
|
G

I
|
I

S
|
S

G

G

A

A

I

I

Q
|
Q

H
|
H

L

I

A

A

G

G

M

M

K

Q

D

D

S

S

K

D

V

I

I

I

V

I

A

A

I

I

N
|
N

K
|
K

D

N

E

E

E

N

A

A

P

P

I

I

F

F

S

E

V

V

A

A

D

D

Y

Y

G

G

L

I

V

V

A

G

D

D

L
|
L

F

Y

V

K

A

V

V

I

P

P

binding flavin-adenine dinucleotide: L180 (≠ Q110), R200 (= R125), M281 (≠ R202), G282 (≠ A203), R307 (= R229), A308 (= A230), Q320 (= Q242), V321 (= V243), G322 (= G244), Q323 (= Q245), T324 (= T246), G337 (= G259), I338 (= I260), S339 (= S261), Q343 (= Q265), H344 (= H266), N358 (= N280), K359 (= K281), L377 (= L299)

Sites not aligning to the query:

binding iron/sulfur cluster: 7, 10, 13, 17, 18, 35, 36, 37, 38, 41, 45, 49

5ow0A Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
38% identity, 78% coverage: 65:306/310 of query aligns to 48:287/292 of 5ow0A

query
sites
5ow0A

L

L

A

S

H

D

N

T

L

L

A

G

E

A

N

N

V

L

A

G

A

S

Q

S

V

L

-

S

-

D

L

L

T

I

I

K

A

R

G

E

N

R

Y

Y

S

E

H

L

I

I

L

L

F

L

P

S

S

T

T

T

A

A

G

I

G

G

K

S

N

G

V

L

A

A

P

G

R

P

V

L

A

A

A

V

K

S

L

L

D

G

V

A

A

P

Q

I

I

L

S

S

D

E

I

V

T

T

K

A

V

I

I

S

S

P

A

D

D

L

T

T

F

I

E

E

R

R

P

S

I

L

Y

Y

A

G

G

S

N

K

A

A

I

V

A

A

T

R

V

Y

Q

K

S

L

A

E

D

S

A

G

T

P

K

L

V

V

I

L

T

T

V

I

R

K

T

R

T

K

G

Y

F

F

D

E

A

A

T

T

A

L

T

E

G

G

G

T

S

T

A

A

T

T

V

E

E

E

N

L

A

P

E

V

G

G

V

P

A

Q

D

-

S

-

G

-

K

K

S

I

A

T

F

L

V

L

G

E

A

E

E

I

I

E

A

E

K

E

N

R

D

T

R

G

P

I

E

P

L

L

T

E

A

D

A

A

K

E

I

V

V

V

S

T

G

G

G
|
G

R
|
R

A
x
G

L

I

G

G

S

S

A

G

E

D

K

N

F

F

T

S

E

-

V

I

M

L

T

K

P

E

L

I

A

A

D

G

K

M

L

L

N

N

A

G

G

A

L

V

G

G

A

A

S
|
S

R
|
R

A

G

A

A

V

V

D

D

A

E

G

G

Y

W

A

M

P

P

N

P

D

G

W

A

Q
|
Q

V
x
I

G
|
G

Q

Q

T
|
T

G

G

K

K

I

I

V

V

A

A

P

P

Q

T

L

V

Y

Y

I

F

A

A

A

V

G
|
G

I
x
V

S
|
S

G

G

A
|
A

I

S

Q
|
Q

H
|
H

L

L

A

A

G

G

M

I

K

S

D

N

S

A

K

K

V

C

I

V

V

I

A

A

I

I

N
|
N

K
|
K

D

D

E

N

E

E

A

A

P

N

I

I

F

F

S

K

V

R

A

A

D

R

Y

F

G

G

L

I

V

V

A

G

D
|
D

L
x
Y

F

K

V

K

A

A

V

V

P

P

E

A

L

L

binding flavin-adenine dinucleotide: G183 (= G201), R184 (= R202), G185 (≠ A203), S209 (= S228), R210 (= R229), Q223 (= Q242), I224 (≠ V243), G225 (= G244), T227 (= T246), G240 (= G259), V241 (≠ I260), S242 (= S261), A244 (= A263), Q246 (= Q265), H247 (= H266), N261 (= N280), K262 (= K281), D279 (= D298), Y280 (≠ L299)

5ol2A The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
36% identity, 83% coverage: 53:310/310 of query aligns to 57:324/331 of 5ol2A

query
sites
5ol2A

G

G

V

A

A

D

K

E

V

V

I

I

V

V

A

V

D

D

S

D

P

E

S

A

L

L

A

A

H

V

N

Y

L

T

A

T

E
|
E

-

P

-

Y

N

T

V

K

A

A

Q

Y

V

E

L

A

T

I

I

K

A

A

G

A

N

D

Y

P

S

I

H

V

I

V

L

L

F

F

P

G

S

A

T

T

A

S

G

I

G

G

K

R

N

D

V

L

A

A

P

P

R

R

V

V

A

S

A

A

K

R

L

I

D

H

V

T

A

G

Q

L

I
x
T

S

A

D

D

I

C

T

T

K

G

V

L

-

A

I

V

S

A

A

E

D

D

T

T

-

K

-

L

F

M

E

T

R
|
R

P

P

I

A

Y

F

A

G

G

G

N

N

A

I

I

M

A

A

T

T

V
x
I

Q

V

S

C

A

K

D

D

A

F

T

R

K

P

V

Q

I

M

T

S

V

T

R

V

T

R

T

P

G

G

F

-

D

-

A

-

T

-

A

V

T

M

G

K

S

N

A

E

T

P

V

D

E

E

N

T

A

K

E

E

G

A

V

V

A

I

D

N

S

R

G

F

K

K

S

V

A

E

F

F

V

N

G

D

A

A

-
x
D

-

K

-

L

-

V

-

Q

-

V

E

Q

I

V

A

I

K

K

N

E

D

A

R

K

P

K

E

Q

-

V

-

K

L

I

T

E

A

D

A

A

K

K

I

I

I

L

V

V

S

S

G

A

G
|
G

R
|
R

A
x
G

L

M

G

G

S

G

A

K

E

E

K

N

F

L

T

-

E

D

V

I

M

L

T

Y

P

E

L

L

A

A

D

E

K

I

L

I

N

G

A

G

G

E

L

V

G

S

A

G

S
|
S

R
|
R

A
|
A

A

T

V

I

D

D

A

A

G

G

Y

W

A

L

P

D

N

K

D

A

W

R

Q
|
Q

V
|
V

G
|
G

Q

Q

T
|
T

G

G

K

K

I

T

V

V

A

R

P

P

Q

D

L

L

Y

Y

I

I

A

A

A

C

G
|
G

I
|
I

S
|
S

G

G

A
|
A

I

I

Q
|
Q

H
|
H

L

I

A

A

G

G

M

M

K

E

D

D

S

A

K

E

V

F

I

I

V

V

A

A

I

I

N
|
N

K
|
K

D

N

E

P

E

E

A

A

P

P

I

I

F

F

S

K

V

Y

A

A

D

D

Y

V

G

G

L

I

V

V

A

G

D
|
D

L
x
V

F

H

V

K

A

V

V

L

P

P

E

E

L

L

T

I

T

S

T

Q

I

L

binding calcium ion: E75 (= E71), D188 (vs. gap)
binding flavin-adenine dinucleotide: T117 (≠ I111), R136 (= R125), I147 (≠ V136), G216 (= G201), R217 (= R202), G218 (≠ A203), S242 (= S228), R243 (= R229), A244 (= A230), Q256 (= Q242), V257 (= V243), G258 (= G244), T260 (= T246), G273 (= G259), I274 (= I260), S275 (= S261), A277 (= A263), Q279 (= Q265), H280 (= H266), N294 (= N280), K295 (= K281), D312 (= D298), V313 (≠ L299)

7qh2A Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
32% identity, 99% coverage: 5:310/310 of query aligns to 15:331/337 of 7qh2A

query
sites
7qh2A

V

V

I

Y

A

V

E

D

H

H

D

I

H

E

A

G

S

Q

I

I

K

H

P

P

A

V

T

T

L

F

N

E

T

L

V

I

-

G

-

K

-

A

-

R

T

E

A

L

A

A

I

A

A

V

C

I

G

G

G

H

E

P

V

V

H

Y

V

A

L

L

V

L

A

M

G

G

A

T

N

N

A

I

A

T

E

E

A

K

G

A

K

D

A

E

A

L

Q

K

I

-

A

Y

G

G

V

V

A

D

K

K

V

V

I

F

V

V

A

Y

D

D

S

K

P

P

S

E

L

L

A

K

H

H

-

F

-

V

-

I

-

E

-

P

-

Y

-

A

N

N

L

V

A

L

E

E

N

D

V

F

A

I

A

E

Q

K

V

V

L

-

T

-

I

-

A

-

G

-

N

K

Y

P

S

S

H

S

I

I

L

L

F

V

P

G

S

A

T

T

A

N

G

V

G

G

K

R

N

S

V

L

A

A

P

P

R

R

V

V

A

A

K

R

L

Y

D

R

V

T

A

G

Q
x
L

I
x
T

S

A

D

D

I

C

T

T

K

I

V

L

I

E

S

M

A

K

D

E

T

N

F

T

E

D

-

L

-

V

-

Q

-

I

R
|
R

P

P

I

A

Y

F

A

G

G

G

N

N

A

I

I

M

A

A

T

Q

V
x
I

Q

V

S

T

A

E

D

N

A

T

T

R

-

P

K

Q

V

F

I

C

T

T

V

V

R

R

T

Y

T

K

G

V

F

F

D

T

A

A

A

P

T

E

A

R

T

V

G

N

G

E

S

P

-

W

A

G

T

D

V

V

E

E

N

-

A

-

E

-

G

-

V

M

A

M

D

D

S

I

G

E

K

K

S

A

A

K

F

L

V

V

G

S

A

A

-

I

-

E

-

V

-

M

E

E

I

V

A

I

K

K

N

K

D

E

R

K

-

G

P

I

E

D

L

L

T

S

A

E

A

A

K

E

I

T

I

I

V

V

S

A

G

V

G

G

R
|
R

A
x
G

L

V

G

-

S

K

A

C

E

E

K

K

F

D

T

L

E

D

V

M

M

I

T

H

P

E

L

F

A

A

D

E

K

K

L

I

N

G

A

A

G

T

L

V

G

A

A

C

S
x
T

R
|
R

A

P

A

G

V

I

D

E

A

A

G

G

Y

W

A

F

P

D

N

A

D

R

W

L

Q
|
Q

V
x
I

G
|
G

Q
x
L

T
x
S

G

G

K

R

I

T

V

V

A

K

P

P

Q

K

L

L

Y

I

I

I

A

A

A

L

G
|
G

I
|
I

S
|
S

G

G

A

A

I

V

Q
|
Q

H

F

L

A

A

A

G

G

M

M

K

Q

D

N

S

S

K

E

V

Y

I

I

V

I

A

A

I

I

N
|
N

K
x
S

D
|
D

E

P

E

K

A

A

P

P

I

I

F

F

S

N

V

I

A

A

D

H

Y

C

G

G

L

M

V

V

A

G

D
|
D

L
|
L

F

Y

V

E

A

I

V

L

P

P

E

E

L

L

T

L

T

T

T

M

I

I

binding flavin-adenine dinucleotide: L125 (≠ Q110), T126 (≠ I111), R144 (= R125), I155 (≠ V136), R224 (= R202), G225 (≠ A203), T249 (≠ S228), R250 (= R229), Q263 (= Q242), I264 (≠ V243), G265 (= G244), L266 (≠ Q245), S267 (≠ T246), G280 (= G259), I281 (= I260), S282 (= S261), Q286 (= Q265), N301 (= N280), S302 (≠ K281), D303 (= D282), D319 (= D298), L320 (= L299)

4kpuA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
32% identity, 82% coverage: 57:310/310 of query aligns to 70:332/338 of 4kpuA

query
sites
4kpuA

V

V

I

Y

V

V

A

C

D

Q

S

D

P

P

S

A

L

F

A

K

H

Y

N

Y

L

S

A

T

E

D

N

E

V

Y

A

T

A

N

Q

A

V

F

L

C

T

E

I

M

A

I

G

D

N

E

Y

Y

-

Q

-

P

S

S

H

S

I

V

L

F

F

I

P

G

S

A

T

T

A

N

G

D

G

G

K

R

N

D

V

L

A

G

P

P

R

R

V

I

A

A

K

R

L

V

D

N

V

T

A

G

Q
x
L

I

C

S

A

D

D

I

C

T

T

K

-

V

I

I

L

S

D

A

A

D

E

T

E

-

D

-

G

-

L

-

I

-

E

F

W

E

T

R
|
R

P

P

I

A

Y

A

A

G

G

G

N

N

A

I

I

M

A

A

T

T

V
x
I

Q

L

S

C

A

K

D

E

A

H

T

R

K

P

V

Q

I

M

-

G

T

T

V

V

R

R

T

P

T

K

G

T

F

F

D

K

A

A

-

M

-

E

-

P

-

D

A

A

T

S

A

R

T

T

G

G

G

E

-

V

-

I

S

N

A

Y

T

T

V

L

E

K

N

N

A

H

E

-

G

-

V

-

A

V

D

D

S

D

G

R

K

V

S

T

A

C

F

I

V

R

G

R

A

E

E

E

I

V

A

V

K

S

N

E

D

G

R

E

P

M

E

A

L

I

T

D

A

D

A

A

K

P

I

F

I

V

V

C

S

S

G

G

G
|
G

R
|
R

A
x
G

L

M

G

K

S

A

A

K

E

E

K

N

F

F

T

S

E

-

V

L

M

L

T

Y

P

D

L

L

A

A

D

H

K

A

L

L

N

G

A

G

G

A

L

V

G

G

A

G

S
|
S

R
|
R

A
|
A

A

A

V

V

D

D

A

E

G

G

Y

F

A

I

P

E

N

H

D

P

W

R

Q
|
Q

V
|
V

G
|
G

Q
|
Q

T
x
S

G

G

K

K

I

T

V

V

A

T

P

P

Q

K

L

I

Y

Y

I

F

A

A

A

C

G
|
G

I
|
I

S
|
S

G

G

A
x
S

I

V

Q
|
Q

H
|
H

L

K

A

A

G

G

M

M

K

S

D

K

S

S

K

D

V

T

I

I

V

V

A

C

I

I

N
|
N

K
|
K

D

D

E

P

E

D

A

A

P

P

I

M

F

F

S

E

V

I

A

S

D

K

Y

Y

G

G

L

I

V

V

A

G

D
|
D

L
x
A

F

L

V

K

A

I

V

L

P

P

E

L

L

L

T

T

T

A

T

K

I

I

binding flavin-adenine dinucleotide: L125 (≠ Q110), R144 (= R125), I155 (≠ V136), G224 (= G201), R225 (= R202), G226 (≠ A203), S250 (= S228), R251 (= R229), A252 (= A230), Q264 (= Q242), V265 (= V243), G266 (= G244), Q267 (= Q245), S268 (≠ T246), G281 (= G259), I282 (= I260), S283 (= S261), S285 (≠ A263), Q287 (= Q265), H288 (= H266), N302 (= N280), K303 (= K281), D320 (= D298), A321 (≠ L299)

P53571 Electron transfer flavoprotein subunit alpha; Alpha-ETF; Electron transfer flavoprotein large subunit; ETFLS from Methylophilus methylotrophus (Bacterium W3A1) (see 2 papers)
33% identity, 98% coverage: 4:306/310 of query aligns to 5:315/321 of P53571

query
sites
P53571

L

L

V

V

I

I

A

A

E

E

H

H

D

R

H

R

A

N

S

D

I

L

K

R

P

P

A

V

T

S

L

L

N

E

T

L

V

I

T

G

A

A

-

N

-

G

I

L

A

K

C

K

G

S

G

G

E

E

V

D

H

K

V

V

L

V

V

V

A

A

G

V

-

I

A

G

N

S

A

Q

A

A

E

D

A

A

G

F

K

V

A

P

A

A

A

L

Q

S

I

V

A

N

G

G

V

V

A

D

K

E

V

L

I

V

V

V

A

V

D

K

S

G

P

S

S

S

L

I

A

D

H

F

N

D

-

P

-

D

-

V

L

F

A

E

E

A

N

S

V

V

A

S

A

A

Q

-

V

-

L

L

T

I

I

A

A

A

G

H

N

N

Y

P

S

S

H

V

I

V

L

L

F

L

P

P

S

H

T

S

A

V

G

D

G

S

K

L

N

G

V

Y

A

A

P

S

R

S

V

L

A

A

A

S

K

K

L

T

D

G

V

Y

A

G

Q

F

I

A

S

T

D

D

I

V

T

Y

K

I

V

V

-

E

I

Y

S

Q

A

G

D

D

T

E

F

L

E

V

R

A

P

T

I

R

Y

G

A

G

G

Y

N

N

A

Q

I

K

A

V

T

N

V

V

Q

E

-

V

-

D

-

F

S

P

A

G

D

K

A

S

T

T

K

V

V

V

I

L

T

T

V

I

R

R

T

P

T

S

G

V

F

F

D

K

A

P

A

L

T

E

A

G

T

A

G

G

G

-

S

S

A

P

T

V

V

V

E

S

N

N

A

V

E

D

G

A

V

P

A

S

D

V

S

Q

G

S

K

R

S

S

A

Q

-

N

-

K

-

D

F

Y

V

V

G

-

A

-

E

E

I

V

A

G

K

G

N

G

D

N

R

D

P

I

E

D

L

I

T

T

A

T

A

V

K

D

I

F

I

I

V

M

S

S

G

I

G

G

R
|
R

A

G

L

I

G

G

-

E

-

T

S

N

A

V

E

E

K

Q

F

F

T

R

E

E

V

-

M

-

T

-

P

-

L

L

A

A

D

D

K

E

L

A

N

G

A

A

G

T

L

L

G

C

A

C

S
|
S

R
|
R

A

P

A

I

V

A

D

D

A

A

G

G

Y

W

A

L

P

P

N

K

D

S

W

R

Q
|
Q

V
|
V

G
|
G

Q
|
Q

T
x
S

G

G

K

K

I

V

V

V

-

G

A

S

P

C

Q

K

L

L

Y

Y

I

V

A

A

A

M

G
|
G

I
|
I

S
|
S

G
|
G

A
x
S

I
|
I

Q
|
Q

H
|
H

L

M

A

A

G

G

M

M

K

K

D

H

S

V

K

P

V

T

I

I

V

I

A

A

I

V

N
|
N

K

T

D

D

E

P

E

G

A

A

P

S

I

I

F

F

S

T

V

I

A

A

D

K

Y

Y

G

G

L

I

V

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A

A

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|
D

L
x
I

F

F

V

D

A

I

V

E

P

E

E

E

L

L

R211 (= R202) binding FAD
SR 236:237 (= SR 228:229) binding FAD
QVGQS 250:254 (≠ QVGQT 242:246) binding FAD
268:275 (vs. 259:266, 88% identical) binding FAD
N289 (= N280) binding FAD
DI 307:308 (≠ DL 298:299) binding FAD

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

7koeB Electron bifurcating flavoprotein fix/etfabcx (see paper)
39% identity, 72% coverage: 87:310/310 of query aligns to 97:327/336 of 7koeB

query
sites
7koeB

I

F

L

L

F

I

P

G

S

A

T

T

A

L

G

E

G

G

K

R

N

E

V

L

A

A

P

G

R

M

V

V

A

A

A

T

K

E

L

L

D

E

V

T

A

G

Q

L

I
x
T

S

A

D

D

I

C

T

T

K

G

V

L

-

D

I

I

S

I

A

P

D

D

T

K

-

K

-

L

-

A

F

M

E

T

R
|
R

P

P

I
x
T

Y

F

A

G

G

G

N

N

A

L

I

M

A

A

T

T

V

I

Q

M

S

C

A

P

D

D

A

H

T

R

K

P

V

Q

I

M

T

A

V

T

R

V

T

R

T

P

G

G

F

V

D

M

A

K

A

E

T

L

A

P

T

P

G

D

G

P

S

E

A

R

T

T

V

G

E

E

N

I

A

I

E

E

G

E

V

E

A

Y

D

D

S

L

G

G

-

T

-

F

-

D

K

K

S

L

A

I

F

E

V

I

G

L

A

E

E

T

I

I

A

P

K

L

N

Q

D

T

R

Q

P

V

E

N

L

L

T

E

A

Y

A

A

K

P

I

V

V

V

S

A

G

G

G
|
G

R
x
K

A
x
G

L

V

G

G

S

G

A

P

E

E

K

G

F

F

T

K

E

K

V

-

M

L

T

K

P

E

L

L

A

A

D

D

K

L

L

L

N

G

A

G

G

E

L

V

G

G

A

A

S
|
S

R
|
R

A
|
A

A

A

V

V

D

K

A

A

G

G

Y

W

A

I

P

S

N

P

D

E

W

H

Q
|
Q

V
|
V

G
|
G

Q
|
Q

T
|
T

G

G

K

K

I

T

V

V

A

R

P

P

Q

V

L

L

Y

Y

I

F

A

A

A

C

G
|
G

I

I

S
|
S

G

G

A

A

I

I

Q
|
Q

H
|
H

L

V

A

V

G

G

M

I

K

K

D

E

S

S

K

E

V

I

I

I

V

V

A

A

I

I

N
|
N

K
x
I

D

D

E

E

E

K

A

A

P

P

I

I

F

F

S

D

V

I

A

A

D

D

Y

I

G

G

L

I

V

V

A

G

D

D

L
|
L

F

H

V

K

A

V

V

V

P

P

E

A

L

L

T

T

T

A

T

K

I

L

binding flavin-adenine dinucleotide: T121 (≠ I111), R140 (= R125), T142 (≠ I127), G219 (= G201), K220 (≠ R202), G221 (≠ A203), S245 (= S228), R246 (= R229), A247 (= A230), Q259 (= Q242), V260 (= V243), G261 (= G244), Q262 (= Q245), T263 (= T246), G276 (= G259), S278 (= S261), Q282 (= Q265), H283 (= H266), N297 (= N280), I298 (≠ K281), L316 (= L299)

3clrD Crystal structure of the r236a etf mutant from m. Methylotrophus (see paper)
33% identity, 98% coverage: 4:306/310 of query aligns to 4:314/319 of 3clrD

query
sites
3clrD

L

L

V

V

I

I

A

A

E

E

H

H

D

R

H

R

A

N

S

D

I

L

K

R

P

P

A

V

T

S

L

L

N

E

T

L

V

I

T

G

A

A

-

N

-

G

I

L

A

K

C

K

G

S

G

G

E

E

V

D

H

K

V

V

L

V

V

V

A

A

G

V

-

I

A

G

N

S

A

Q

A

A

E

D

A

A

G

F

K

V

A

P

A

A

A

L

Q

S

I

V

A

N

G

G

V

V

A

D

K

E

V

L

I

V

V

V

A

V

D

K

S

G

P

S

S

S

L

I

A

D

H

F

N

D

-

P

-

D

-

V

L

F

A

E

E

A

N

S

V

V

A

S

A

A

Q

-

V

-

L

L

T

I

I

A

A

A

G

H

N

N

Y

P

S

S

H

V

I

V

L

L

F

L

P

P

S

H

T

S

A

V

G

D

G

S

K

L

N

G

V

Y

A

A

P

S

R

S

V

L

A

A

A

S

K

K

L

T

D

G

V

Y

A

G

Q

F

I

A

S

T

D

D

I

V

T

Y

K

I

V

V

-

E

I

Y

S

Q

A

G

D

D

T

E

F

L

E

V

R

A

P

T

I

R

Y

G

A

G

G

Y

N

N

A

Q

I

K

A

V

T

N

V

V

Q

E

-

V

-

D

-

F

S

P

A

G

D

K

A

S

T

T

K

V

V

V

I

L

T

T

V

I

R

R

T

P

T

S

G

V

F

F

D

K

A

P

A

L

T

E

A

G

T

A

G

G

G

-

S

S

A

P

T

V

V

V

E

S

N

N

A

V

E

D

G

A

V

P

A

S

D

V

S

Q

G

S

K

R

S

S

A

Q

-

N

-

K

-

D

F

Y

V

V

G

-

A

-

E

E

I

V

A

G

K

G

N

G

D

N

R

D

P

I

E

D

L

I

T

T

A

T

A

V

K

D

I

F

I

I

V

M

S

S

G

I

G
|
G

R
|
R

A
x
G

L

I

G

G

-

E

-

T

S

N

A

V

E

E

K

Q

F

F

T

R

E

E

V

-

M

-

T

-

P

-

L

L

A

A

D

D

K

E

L

A

N

G

A

A

G

T

L

L

G

C

A

C

S
|
S

R
x
A

A
x
P

A

I

V

A

D

D

A

A

G

G

Y

W

A

L

P

P

N

K

D

S

W

R

Q
|
Q

V
|
V

G
|
G

Q
|
Q

T
x
S

G

G

K

K

I

V

V

V

-

G

A

S

P

C

Q

K

L

L

Y

Y

I

V

A

A

A

M

G
|
G

I
|
I

S
|
S

G

G

A
x
S

I

I

Q
|
Q

H
|
H

L

M

A

A

G

G

M

M

K

K

D

H

S

V

K

P

V

T

I

I

V

I

A

A

I

V

N
|
N

K
x
T

D

D

E

P

E

G

A

A

P

S

I

I

F

F

S

T

V

I

A

A

D

K

Y

Y

G

G

L

I

V

V

A

A

D
|
D

L
x
I

F

F

V

D

A

I

V

E

P

E

E

E

L

L

binding flavin-adenine dinucleotide: G209 (= G201), R210 (= R202), G211 (≠ A203), S235 (= S228), A236 (≠ R229), P237 (≠ A230), Q249 (= Q242), V250 (= V243), G251 (= G244), Q252 (= Q245), S253 (≠ T246), G267 (= G259), I268 (= I260), S269 (= S261), S271 (≠ A263), Q273 (= Q265), H274 (= H266), N288 (= N280), T289 (≠ K281), D306 (= D298), I307 (≠ L299)

Query Sequence

>WP_011812370.1 NCBI__GCF_000015565.1:WP_011812370.1
MAALVIAEHDHASIKPATLNTVTAAIACGGEVHVLVAGANAAEAGKAAAQIAGVAKVIVA
DSPSLAHNLAENVAAQVLTIAGNYSHILFPSTAGGKNVAPRVAAKLDVAQISDITKVISA
DTFERPIYAGNAIATVQSADATKVITVRTTGFDAATATGGSATVENAEGVADSGKSAFVG
AEIAKNDRPELTAAKIIVSGGRALGSAEKFTEVMTPLADKLNAGLGASRAAVDAGYAPND
WQVGQTGKIVAPQLYIAAGISGAIQHLAGMKDSKVIVAINKDEEAPIFSVADYGLVADLF
VAVPELTTTI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory