SitesBLAST
Comparing WP_011812871.1 NCBI__GCF_000015585.1:WP_011812871.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3pm9A Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
27% identity, 29% coverage: 171:544/1286 of query aligns to 43:357/465 of 3pm9A
- active site: A149 (= A286), L159 (≠ T296)
- binding flavin-adenine dinucleotide: P69 (= P197), Q70 (≠ R198), G71 (= G199), G72 (= G200), N73 (≠ G201), T74 (= T202), G75 (= G203), L76 (≠ Y204), G79 (= G207), Q80 (≠ A208), L91 (≠ T220), L133 (≠ P270), G134 (≠ T271), A135 (≠ S272), C139 (≠ S276), T140 (= T277), G142 (= G279), G143 (= G280), S146 (= S283), T147 (≠ M284), A149 (= A286), G150 (= G287), E200 (= E383), G201 (= G384), I205 (≠ L388), I206 (= I389)
Sites not aligning to the query:
6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
22% identity, 29% coverage: 171:539/1286 of query aligns to 49:358/466 of 6lpxA
- binding 2-oxoglutaric acid: R333 (= R513), T337 (≠ A517), K348 (≠ N529)
- binding flavin-adenine dinucleotide: P75 (= P197), Q76 (≠ R198), G77 (= G199), G78 (= G200), N79 (≠ G201), T80 (= T202), G81 (= G203), M82 (≠ Y204), G85 (= G207), S86 (≠ A208), L139 (≠ P270), G140 (≠ T271), A141 (≠ S272), C145 (≠ S276), G149 (= G280), N150 (= N281), A152 (≠ S283), T153 (≠ M284), G157 (= G288), G207 (= G384), I212 (= I389)
Sites not aligning to the query:
6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
22% identity, 29% coverage: 171:539/1286 of query aligns to 49:358/466 of 6lpuA
- binding flavin-adenine dinucleotide: P75 (= P197), G77 (= G199), G78 (= G200), N79 (≠ G201), T80 (= T202), G81 (= G203), G85 (= G207), S86 (≠ A208), L139 (≠ P270), G140 (≠ T271), A141 (≠ S272), C145 (≠ S276), H146 (≠ T277), G148 (= G279), G149 (= G280), N150 (= N281), A152 (≠ S283), T153 (≠ M284), A155 (= A286), E206 (= E383), G207 (= G384), I211 (≠ L388), I212 (= I389)
- binding (2s)-2-hydroxypentanedioic acid: R333 (= R513), T337 (≠ A517), K348 (≠ N529)
Sites not aligning to the query:
6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal) (see paper)
22% identity, 29% coverage: 171:539/1286 of query aligns to 49:358/466 of 6lpqA
- binding flavin-adenine dinucleotide: P75 (= P197), G77 (= G199), G78 (= G200), N79 (≠ G201), T80 (= T202), G81 (= G203), G85 (= G207), S86 (≠ A208), L139 (≠ P270), G140 (≠ T271), A141 (≠ S272), C145 (≠ S276), H146 (≠ T277), G149 (= G280), N150 (= N281), A152 (≠ S283), T153 (≠ M284), A155 (= A286), G157 (= G288), E206 (= E383), G207 (= G384), I211 (≠ L388), I212 (= I389)
- binding d-malate: M82 (≠ Y204), R333 (= R513), T337 (≠ A517), K348 (≠ N529)
Sites not aligning to the query:
6lppA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-2-hydroxyglutarate (d-2-hg) (see paper)
22% identity, 29% coverage: 171:539/1286 of query aligns to 49:358/466 of 6lppA
- binding (2R)-2-hydroxypentanedioic acid: R333 (= R513), T337 (≠ A517), K348 (≠ N529)
- binding flavin-adenine dinucleotide: P75 (= P197), G77 (= G199), G78 (= G200), N79 (≠ G201), T80 (= T202), G81 (= G203), M82 (≠ Y204), G85 (= G207), S86 (≠ A208), L139 (≠ P270), G140 (≠ T271), A141 (≠ S272), C145 (≠ S276), G149 (= G280), N150 (= N281), A152 (≠ S283), T153 (≠ M284), A155 (= A286), G157 (= G288), G207 (= G384), I212 (= I389)
Sites not aligning to the query:
6lpnB Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
22% identity, 29% coverage: 171:539/1286 of query aligns to 50:359/467 of 6lpnB
- binding flavin-adenine dinucleotide: P76 (= P197), G78 (= G199), G79 (= G200), N80 (≠ G201), T81 (= T202), G82 (= G203), M83 (≠ Y204), G86 (= G207), S87 (≠ A208), L140 (≠ P270), A142 (≠ S272), C146 (≠ S276), H147 (≠ T277), G150 (= G280), N151 (= N281), A153 (≠ S283), T154 (≠ M284), G208 (= G384), I212 (≠ L388), I213 (= I389)
Sites not aligning to the query:
Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
22% identity, 29% coverage: 171:539/1286 of query aligns to 102:411/521 of Q8N465
- S109 (≠ E178) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
- N127 (≠ I196) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
- G131 (= G200) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
- I147 (≠ V217) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
- M153 (≠ L223) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
- C172 (≠ A250) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
- P189 (≠ A267) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
- A205 (≠ S283) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
- A231 (= A309) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
- G233 (≠ A311) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734
- D375 (≠ S502) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
- R386 (= R513) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- T390 (≠ A517) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Significantly reduced catalytic activity.
- V399 (≠ K527) to M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
- K401 (≠ N529) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Loss of catalytic activity.
Sites not aligning to the query:
- 15 R → G: in dbSNP:rs4675887
- 169:521 natural variant: Missing (in D2HGA1; uncertain significance)
- 400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)
- 419 R → H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
- 426 A → T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
- 434 binding Zn(2+); H→A: Loss of catalytic activity.
- 436 G → V: slight reduction in catalytic activity
- 439 N → D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
- 441 binding Zn(2+); H→A: Loss of catalytic activity.
- 443 binding (R)-2-hydroxyglutarate; N→A: Significantly reduced catalytic activity.
- 444 V → A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
- 446 A → V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
- 475 binding Zn(2+); E→A: Loss of catalytic activity.
- 476 binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; H→A: Loss of catalytic activity.
- 477 G → R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640
8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
24% identity, 35% coverage: 154:604/1286 of query aligns to 25:406/456 of 8jdsA
- binding flavin-adenine dinucleotide: E32 (≠ A161), P68 (= P197), G70 (= G199), T71 (≠ G200), G72 (= G201), T73 (= T202), G74 (= G203), G78 (= G207), V79 (≠ A208), L90 (≠ T220), P132 (= P270), G133 (≠ T271), A134 (≠ S272), G140 (= G280), M141 (≠ N281), A143 (≠ S283), T144 (≠ M284), A146 (= A286), S147 (≠ G287), E200 (= E383), G201 (= G384), I206 (= I389), W323 (vs. gap)
- binding manganese (ii) ion: H370 (≠ Y568), H377 (≠ E575)
- binding pyruvic acid: R319 (= R513), H370 (≠ Y568), H377 (≠ E575)
Sites not aligning to the query:
8jdrA Crystal structure of h405a mldhd in complex with d-2-hydroxy-3-methyl- valeric acid (see paper)
24% identity, 35% coverage: 154:604/1286 of query aligns to 25:406/456 of 8jdrA
- binding flavin-adenine dinucleotide: P68 (= P197), G70 (= G199), T71 (≠ G200), G72 (= G201), T73 (= T202), G74 (= G203), G78 (= G207), V79 (≠ A208), L90 (≠ T220), P132 (= P270), G133 (≠ T271), A134 (≠ S272), G140 (= G280), M141 (≠ N281), A143 (≠ S283), T144 (≠ M284), A146 (= A286), S147 (≠ G287), E200 (= E383), G201 (= G384), I206 (= I389), Y324 (vs. gap), H370 (≠ Y568)
- binding (2R,3S)-3-methyl-2-oxidanyl-pentanoic acid: R319 (= R513), W323 (vs. gap)
Sites not aligning to the query:
8jdqA Crystal structure of h405a mldhd in complex with d-2-hydroxyisocaproic acid (see paper)
24% identity, 35% coverage: 154:604/1286 of query aligns to 25:406/456 of 8jdqA
- binding (2R)-2-hydroxy-4-methylpentanoic acid: R319 (= R513), W323 (vs. gap), H370 (≠ Y568)
- binding flavin-adenine dinucleotide: P68 (= P197), G70 (= G199), T71 (≠ G200), G72 (= G201), T73 (= T202), G74 (= G203), G78 (= G207), V79 (≠ A208), L90 (≠ T220), P132 (= P270), G133 (≠ T271), A134 (≠ S272), G140 (= G280), M141 (≠ N281), A143 (≠ S283), T144 (≠ M284), A146 (= A286), S147 (≠ G287), E200 (= E383), G201 (= G384), I206 (= I389), H370 (≠ Y568)
Sites not aligning to the query:
8jdoA Crystal structure of h405a mldhd in complex with d-2-hydroxyhexanoic acid (see paper)
24% identity, 35% coverage: 154:604/1286 of query aligns to 25:406/456 of 8jdoA
- binding (2R)-2-hydroxyhexanoic acid: R319 (= R513), W323 (vs. gap)
- binding flavin-adenine dinucleotide: P68 (= P197), G70 (= G199), T71 (≠ G200), G72 (= G201), T73 (= T202), G74 (= G203), G78 (= G207), V79 (≠ A208), L90 (≠ T220), P132 (= P270), G133 (≠ T271), A134 (≠ S272), G140 (= G280), M141 (≠ N281), A143 (≠ S283), T144 (≠ M284), A146 (= A286), S147 (≠ G287), E200 (= E383), G201 (= G384), I206 (= I389), Y324 (vs. gap), H370 (≠ Y568)
Sites not aligning to the query:
8jdnA Crystal structure of h405a mldhd in complex with d-2-hydroxyvaleric acid (see paper)
24% identity, 35% coverage: 154:604/1286 of query aligns to 25:406/456 of 8jdnA
- binding flavin-adenine dinucleotide: P68 (= P197), G70 (= G199), T71 (≠ G200), G72 (= G201), T73 (= T202), G74 (= G203), G78 (= G207), V79 (≠ A208), L90 (≠ T220), P132 (= P270), G133 (≠ T271), A134 (≠ S272), G140 (= G280), M141 (≠ N281), A143 (≠ S283), T144 (≠ M284), A146 (= A286), S147 (≠ G287), E200 (= E383), G201 (= G384), I206 (= I389), H370 (≠ Y568)
- binding (2R)-2-oxidanylpentanoic acid: R319 (= R513), W323 (vs. gap)
Sites not aligning to the query:
8jdgA Crystal structure of h405a mldhd in complex with d-2-hydroxybutanoic acid (see paper)
24% identity, 35% coverage: 154:604/1286 of query aligns to 25:406/456 of 8jdgA
- binding flavin-adenine dinucleotide: P68 (= P197), G70 (= G199), T71 (≠ G200), G72 (= G201), T73 (= T202), G74 (= G203), G78 (= G207), V79 (≠ A208), L90 (≠ T220), P132 (= P270), G133 (≠ T271), A134 (≠ S272), G140 (= G280), M141 (≠ N281), A143 (≠ S283), T144 (≠ M284), A146 (= A286), S147 (≠ G287), E200 (= E383), G201 (= G384), I206 (= I389), H370 (≠ Y568)
- binding (2R)-2-oxidanylbutanoic acid: R319 (= R513)
Sites not aligning to the query:
8jdbA Crystal structure of h405a mldhd in complex with d-2-hydroxyoctanoic acid (see paper)
24% identity, 35% coverage: 154:604/1286 of query aligns to 25:406/456 of 8jdbA
- binding flavin-adenine dinucleotide: P68 (= P197), G70 (= G199), T71 (≠ G200), G72 (= G201), T73 (= T202), G74 (= G203), G78 (= G207), V79 (≠ A208), L90 (≠ T220), P132 (= P270), G133 (≠ T271), A134 (≠ S272), G140 (= G280), M141 (≠ N281), A143 (≠ S283), T144 (≠ M284), A146 (= A286), S147 (≠ G287), E200 (= E383), G201 (= G384), I206 (= I389), Y324 (vs. gap), H370 (≠ Y568)
- binding (2R)-2-oxidanyloctanoic acid: V75 (≠ Y204), R319 (= R513), W323 (vs. gap)
Sites not aligning to the query:
8jdvA Crystal structure of mldhd in complex with 2-ketohexanoic acid (see paper)
24% identity, 35% coverage: 154:604/1286 of query aligns to 25:404/454 of 8jdvA
- binding 2-Ketohexanoic acid: V75 (≠ Y204), R317 (= R513), W321 (vs. gap), H368 (≠ Y568), H375 (≠ E575)
- binding flavin-adenine dinucleotide: P68 (= P197), G70 (= G199), T71 (≠ G200), G72 (= G201), T73 (= T202), G74 (= G203), G78 (= G207), V79 (≠ A208), L90 (≠ T220), P132 (= P270), G133 (≠ T271), A134 (≠ S272), G140 (= G280), M141 (≠ N281), A143 (≠ S283), T144 (≠ M284), A146 (= A286), S147 (≠ G287), E200 (= E383), G201 (= G384), I206 (= I389), W321 (vs. gap), Y322 (vs. gap)
- binding manganese (ii) ion: H368 (≠ Y568), H375 (≠ E575)
Sites not aligning to the query:
8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid (see paper)
24% identity, 35% coverage: 154:604/1286 of query aligns to 25:405/455 of 8jduA
- binding 2-oxopentanoic acid: R318 (= R513), W322 (vs. gap), H369 (≠ Y568), H376 (≠ E575)
- binding flavin-adenine dinucleotide: P68 (= P197), G70 (= G199), T71 (≠ G200), G72 (= G201), T73 (= T202), G74 (= G203), G78 (= G207), V79 (≠ A208), L90 (≠ T220), P132 (= P270), G133 (≠ T271), A134 (≠ S272), G140 (= G280), M141 (≠ N281), A143 (≠ S283), T144 (≠ M284), A146 (= A286), S147 (≠ G287), E200 (= E383), G201 (= G384), I206 (= I389), W322 (vs. gap)
- binding manganese (ii) ion: H369 (≠ Y568), H376 (≠ E575)
Sites not aligning to the query:
8jdeA Crystal structure of mldhd in complex with d-lactate (see paper)
24% identity, 35% coverage: 154:604/1286 of query aligns to 25:405/455 of 8jdeA
- binding flavin-adenine dinucleotide: P68 (= P197), G70 (= G199), T71 (≠ G200), G72 (= G201), T73 (= T202), G74 (= G203), G78 (= G207), V79 (≠ A208), L90 (≠ T220), P132 (= P270), G133 (≠ T271), A134 (≠ S272), G140 (= G280), M141 (≠ N281), A143 (≠ S283), T144 (≠ M284), A146 (= A286), S147 (≠ G287), E200 (= E383), G201 (= G384), I206 (= I389), W322 (vs. gap)
- binding lactic acid: R318 (= R513), H369 (≠ Y568), H376 (≠ E575)
- binding manganese (ii) ion: H369 (≠ Y568), H376 (≠ E575)
Sites not aligning to the query:
8jdpA Crystal structure of h405a mldhd in complex with d-2-hydroxyisovaleric acid (see paper)
25% identity, 35% coverage: 154:604/1286 of query aligns to 25:405/455 of 8jdpA
- binding flavin-adenine dinucleotide: P68 (= P197), G70 (= G199), T71 (≠ G200), G72 (= G201), T73 (= T202), G74 (= G203), G78 (= G207), V79 (≠ A208), L90 (≠ T220), P132 (= P270), G133 (≠ T271), A134 (≠ S272), G140 (= G280), M141 (≠ N281), A143 (≠ S283), T144 (≠ M284), A146 (= A286), S147 (≠ G287), E200 (= E383), G201 (= G384), I206 (= I389), H369 (≠ Y568)
- binding deaminohydroxyvaline: R319 (= R513)
Sites not aligning to the query:
8jdtA Crystal structure of mldhd in complex with 2-ketobutanoic acid (see paper)
24% identity, 35% coverage: 154:604/1286 of query aligns to 25:405/455 of 8jdtA
- binding 2-ketobutyric acid: R318 (= R513), H369 (≠ Y568), H376 (≠ E575)
- binding flavin-adenine dinucleotide: P68 (= P197), G70 (= G199), T71 (≠ G200), G72 (= G201), T73 (= T202), G74 (= G203), G78 (= G207), V79 (≠ A208), L90 (≠ T220), P132 (= P270), G133 (≠ T271), A134 (≠ S272), G140 (= G280), M141 (≠ N281), A143 (≠ S283), T144 (≠ M284), A146 (= A286), S147 (≠ G287), E200 (= E383), G201 (= G384), I206 (= I389), W322 (vs. gap)
- binding manganese (ii) ion: H369 (≠ Y568), H376 (≠ E575)
Sites not aligning to the query:
8jdxA Crystal structure of mldhd in complex with 2-ketoisovaleric acid (see paper)
24% identity, 35% coverage: 154:604/1286 of query aligns to 25:405/455 of 8jdxA
- binding flavin-adenine dinucleotide: E32 (≠ A161), P68 (= P197), G70 (= G199), T71 (≠ G200), G72 (= G201), T73 (= T202), G74 (= G203), G78 (= G207), V79 (≠ A208), L90 (≠ T220), P132 (= P270), G133 (≠ T271), A134 (≠ S272), G140 (= G280), M141 (≠ N281), A143 (≠ S283), T144 (≠ M284), A146 (= A286), S147 (≠ G287), E200 (= E383), G201 (= G384), I206 (= I389), W322 (vs. gap)
- binding 3-methyl-2-oxobutanoic acid: R318 (= R513), H369 (≠ Y568), H376 (≠ E575)
- binding manganese (ii) ion: H369 (≠ Y568), H376 (≠ E575)
Sites not aligning to the query:
Query Sequence
>WP_011812871.1 NCBI__GCF_000015585.1:WP_011812871.1
MAAINQEPTRIREIPYNYTSFSDREIVVRFLGEETWDLLNELRGERRTGISARMLFEVLG
DLWVVSRNPYIQDDLINTPKRLRALVSSMHHRVDQIFARANGNQKGIDLGERARRAVQEF
EQHYRRQRQRRQRIKRRLARFTRSDNICFDGLSRVSHATDATDWRVEIPFLVLTPDTEEE
IAPLVATCIEEGLTIIPRGGGTGYTGGAVPLDGDSAVINTEKLDAISPVEEVDLPGVDTP
VSTIHVGAGAVTRRVSERAEAHGRVFAVDPTSQDASTIGGNISMNAGGKKAVLWGTTLDN
IVSYRMITAEAKWIEVERLDHNLGKLDDLDTVRFRVTRYARDGQTQEGEPEILSFSGTSF
RKPGLGKDVTDKFLGGLPGVQKEGCDGLITSARMLVHRMPEHQRTVCLEFYGDDLHEAVP
AITEIRDDLLADEQVALAGLEHLDERYIQAVDYSPKANRSEIPKMILLADLASDHPDAVG
EAASRIVRLANQRNGEGFIAVSEEAQRRFWAERSRTAAIASHTNAFKINEDVVIPLERLA
EYTEGVERINVEQSMRNKLAMLSELLDYLGGDMPEVRQVKDFEDSEEGDRILADKKAAAR
DHLSQVRERWQQILEAFDAAAADRDDLLDDTARADLRAGDRIIDLLLRRSLRISYRREVE
RPLEEFFKGRDLQPVRARFTEIHDRVLRSRLFVALHMHAGDGNVHTNIPVNSGDYGMMRE
AERIVDRIMELAQSLGGVITGEHGVGLTKMQYLDPEQVQRFAEFIDRVDPEGRFNRGKLM
PGSGLENAYTPSLFLVEEEALILEESELGELNRDIKDCLRCGKCKPVCSTHVPRANLLYS
PRNKILAAGLIIEAFLYEEQTRRGLSLRHFDVMNDVADHCTICHKCLKPCPVNIDFGDVT
IRLRDILQRRKQRRTRLQTRVAMGFLNLTDPRLIKALRLGMARFGFAAQRQVSQGLRRMG
VFPLKRRPGSTTERSSIPAQAVHFLEKPMPAAVPPQGMRQILGAEESDHIPIIRDPQRTD
ENSEAVFYFPGCGPERLFSQVGMATLAMLHDSGVKTVLPPGYLCCGYPQHAGGYIDRAKE
ITTANRVLLHRVANTLNYMDIQTVVVSCGTCISQLELYEFNRIFPGCRLIDIHEFLLEKG
YRLEGSKETQYLYHDPCHPSIKTQNPTGVASELMGQPVQLSDRCCGESGIFAVSRPDISS
QVRHRKQEEIRQGVQSLTGAARAEDGNVRMLTTCPSCLQGLSRYREDTGVEADYIVTEMA
RELLGEDWQERFTEQARKGSLEKVLL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory