SitesBLAST
Comparing WP_011812925.1 NCBI__GCF_000015585.1:WP_011812925.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P04805 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Escherichia coli (strain K12) (see 4 papers)
37% identity, 99% coverage: 4:475/477 of query aligns to 1:470/471 of P04805
- C98 (= C109) mutation to S: 10-fold decrease in activity. Strong decrease in zinc content.
- C100 (= C111) mutation to S: Loss of activity. Strong decrease in zinc content.; mutation to Y: Does not prevent zinc binding. Reduces only 2-fold the binding affinity for tRNA(Glu), but reduces more than 10-fold the affinity for glutamate in the presence of tRNA(Glu).
- C125 (= C136) mutation to S: Loss of activity. Strong decrease in zinc content.
- H127 (≠ L139) mutation to Q: 10-fold decrease in activity. Strong decrease in zinc content.
- H129 (≠ A141) mutation to Q: No change in activity or in zinc content.
- H131 (≠ E143) mutation to Q: No change in activity or in zinc content.
- H132 (≠ R148) mutation to Q: No change in activity or in zinc content.
- C138 (≠ P154) mutation to S: No change in activity or in zinc content.
- S239 (= S255) modified: Phosphoserine; mutation to D: Does not aminoacylate tRNA(Glu), not phosphorylated by HipA.
8i9iA Glutamyl-tRNA synthetase from escherichia coli bound to glutamate and zinc
37% identity, 98% coverage: 4:472/477 of query aligns to 1:467/468 of 8i9iA
4griB Crystal structure of a glutamyl-tRNA synthetase glurs from borrelia burgdorferi bound to glutamic acid and zinc (see paper)
32% identity, 97% coverage: 5:465/477 of query aligns to 1:478/485 of 4griB
- active site: S9 (= S13), K253 (= K256)
- binding glutamic acid: R5 (= R9), A7 (= A11), S9 (= S13), E41 (= E45), Y194 (= Y197), R212 (= R215), W216 (≠ H219)
- binding zinc ion: C105 (= C109), C107 (= C111), Y128 (= Y132), C132 (= C136)
8vc5A Crystal structure of glutamyl-tRNA synthetase glurs from pseudomonas aeruginosa (zinc bound)
34% identity, 97% coverage: 5:468/477 of query aligns to 3:468/488 of 8vc5A
Q8DLI5 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1) (see paper)
37% identity, 98% coverage: 4:472/477 of query aligns to 1:482/485 of Q8DLI5
- R6 (= R9) binding L-glutamate
- Y192 (= Y197) binding L-glutamate
2cfoA Non-discriminating glutamyl-tRNA synthetase from thermosynechococcus elongatus in complex with glu (see paper)
36% identity, 98% coverage: 5:472/477 of query aligns to 1:481/484 of 2cfoA
3al0C Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
34% identity, 96% coverage: 6:465/477 of query aligns to 103:558/564 of 3al0C
- active site: S110 (= S13), K335 (= K256)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R106 (= R9), A108 (= A11), P109 (= P12), G118 (= G21), T122 (= T25), E142 (= E45), Y276 (= Y197), R294 (= R215), G295 (= G216), D297 (= D218), H298 (= H219), L324 (= L245), I325 (= I246), L333 (= L254)
- binding : T144 (= T47), D145 (= D48), R148 (= R51), Y208 (≠ C111), P213 (= P133), K252 (≠ H173), M255 (≠ Q176), I266 (= I187), K269 (≠ R190), S270 (≠ A191), Y276 (= Y197), D297 (= D218), H298 (= H219), L299 (= L220), S300 (≠ T221), N301 (= N222), K304 (≠ R225), R330 (≠ G251), P332 (= P253), G363 (= G284), W364 (≠ H285), R365 (≠ T286), E370 (≠ A291), S387 (= S308), K389 (≠ A310), V391 (≠ S312), I392 (≠ R313), K397 (≠ H318), W400 (≠ A326), R407 (= R333), E446 (≠ G359), K447 (≠ N360), Q453 (≠ E366), I457 (≠ W370), R509 (≠ K412), K520 (≠ Q428), Q524 (≠ M431), R527 (= R434), V535 (≠ S442), T536 (≠ G443), G538 (≠ E445), L539 (= L446)
2cv2A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu) and an enzyme inhibitor, glu-ams (see paper)
33% identity, 96% coverage: 6:465/477 of query aligns to 2:466/468 of 2cv2A
- active site: K246 (= K256)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R5 (= R9), A7 (= A11), S9 (= S13), G17 (= G21), I21 (≠ T25), E41 (= E45), Y187 (= Y197), R205 (= R215), A206 (≠ G216), E208 (≠ D218), W209 (≠ H219), L235 (= L245), L236 (≠ I246)
- binding : S9 (= S13), T43 (= T47), D44 (= D48), R47 (= R51), V145 (≠ A155), R163 (≠ H173), Y168 (≠ F178), E172 (≠ D182), V177 (≠ I187), K180 (≠ R190), S181 (≠ A191), Y187 (= Y197), E207 (≠ D217), E208 (≠ D218), W209 (≠ H219), V211 (≠ T221), R237 (≠ T247), K241 (≠ G251), L272 (≠ R282), M273 (≠ V283), G274 (= G284), E282 (≠ A291), S299 (= S308), P303 (≠ S312), V304 (≠ R313), K309 (≠ H318), W312 (= W322), R319 (= R333), P357 (≠ G359), R358 (≠ N360), R417 (≠ E416), Q432 (≠ M431), R435 (= R434), L442 (≠ C441), E443 (≠ S442), T444 (≠ G443), G446 (≠ E445), L447 (= L446), F448 (≠ Q447)
2cv1A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu), atp, and an analog of l-glutamate: a quaternary complex
33% identity, 96% coverage: 6:465/477 of query aligns to 2:466/468 of 2cv1A
- active site: K246 (= K256)
- binding adenosine-5'-triphosphate: P8 (= P12), S9 (= S13), G17 (= G21), T18 (≠ N22), I21 (≠ T25), R47 (= R51), A206 (≠ G216), W209 (≠ H219), L235 (= L245), L236 (≠ I246)
- binding (4s)-4-amino-5-hydroxypentanoic acid: R5 (= R9), A7 (= A11), E41 (= E45), Y187 (= Y197), R205 (= R215), W209 (≠ H219)
- binding : S9 (= S13), E41 (= E45), T43 (= T47), D44 (= D48), R47 (= R51), V145 (≠ A155), R163 (≠ H173), V166 (≠ Q176), E172 (≠ D182), V177 (≠ I187), K180 (≠ R190), S181 (≠ A191), Y187 (= Y197), E207 (≠ D217), E208 (≠ D218), W209 (≠ H219), V211 (≠ T221), R237 (≠ T247), K241 (≠ G251), K243 (≠ P253), M273 (≠ V283), G274 (= G284), S276 (≠ T286), E282 (≠ A291), S299 (= S308), P303 (≠ S312), V304 (≠ R313), K309 (≠ H318), W312 (= W322), R319 (= R333), P357 (≠ G359), R358 (≠ N360), R417 (≠ E416), L427 (≠ G426), Q432 (≠ M431), R435 (= R434), L442 (≠ C441), E443 (≠ S442), T444 (≠ G443), G446 (≠ E445), L447 (= L446), F448 (≠ Q447)
2cuzA Glutamyl-tRNA synthetase from thermus thermophilus in complex with l- glutamate (see paper)
33% identity, 96% coverage: 6:465/477 of query aligns to 2:466/468 of 2cuzA
1n78A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with tRNA(glu) and glutamol-amp. (see paper)
33% identity, 96% coverage: 6:465/477 of query aligns to 2:466/468 of 1n78A
- active site: K246 (= K256)
- binding glutamol-amp: R5 (= R9), A7 (= A11), P8 (= P12), S9 (= S13), G17 (= G21), T18 (≠ N22), I21 (≠ T25), E41 (= E45), Y187 (= Y197), N191 (= N201), R205 (= R215), A206 (≠ G216), E208 (≠ D218), W209 (≠ H219), L235 (= L245), L236 (≠ I246)
- binding : S9 (= S13), T43 (= T47), D44 (= D48), R47 (= R51), V145 (≠ A155), R163 (≠ H173), V166 (≠ Q176), Y168 (≠ F178), E172 (≠ D182), V177 (≠ I187), K180 (≠ R190), S181 (≠ A191), Y187 (= Y197), E207 (≠ D217), E208 (≠ D218), W209 (≠ H219), L210 (= L220), V211 (≠ T221), R237 (≠ T247), K241 (≠ G251), M273 (≠ V283), G274 (= G284), E282 (≠ A291), R297 (= R306), P303 (≠ S312), V304 (≠ R313), K309 (≠ H318), W312 (= W322), R319 (= R333), P357 (≠ G359), R358 (≠ N360), R417 (≠ E416), L427 (≠ G426), Q432 (≠ M431), R435 (= R434), L442 (≠ C441), E443 (≠ S442), T444 (≠ G443), G446 (≠ E445), L447 (= L446), F448 (≠ Q447)
1j09A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with atp and glu (see paper)
33% identity, 96% coverage: 6:465/477 of query aligns to 2:466/468 of 1j09A
- active site: K246 (= K256)
- binding adenosine-5'-triphosphate: H15 (= H19), E208 (≠ D218), L235 (= L245), L236 (≠ I246), K243 (≠ P253), I244 (≠ L254), S245 (= S255), K246 (= K256), R247 (= R257)
- binding glutamic acid: R5 (= R9), A7 (= A11), S9 (= S13), E41 (= E45), Y187 (= Y197), N191 (= N201), R205 (= R215), W209 (≠ H219)
P27000 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
33% identity, 96% coverage: 6:465/477 of query aligns to 2:466/468 of P27000
- R358 (≠ N360) mutation to Q: Reduces affinity for tRNA and abolishes the ability to discriminate between tRNA(Glu) and tRNA(Gln).
1g59A Glutamyl-tRNA synthetase complexed with tRNA(glu). (see paper)
33% identity, 96% coverage: 6:465/477 of query aligns to 2:466/468 of 1g59A
- binding : D44 (= D48), R45 (= R49), A46 (≠ E50), R47 (= R51), P109 (≠ E113), V145 (≠ A155), R163 (≠ H173), V166 (≠ Q176), E172 (≠ D182), V177 (≠ I187), K180 (≠ R190), S181 (≠ A191), D182 (= D192), E207 (≠ D217), E208 (≠ D218), R237 (≠ T247), K241 (≠ G251), T242 (≠ A252), K243 (≠ P253), M273 (≠ V283), G274 (= G284), E282 (≠ A291), S299 (= S308), L300 (≠ T309), P303 (≠ S312), V304 (≠ R313), K309 (≠ H318), W312 (= W322), R319 (= R333), P357 (≠ G359), R358 (≠ N360), R417 (≠ E416), K426 (= K425), L427 (≠ G426), Q432 (≠ M431), R435 (= R434), L442 (≠ C441), E443 (≠ S442), T444 (≠ G443), P445 (= P444), G446 (≠ E445), L447 (= L446), F448 (≠ Q447)
4g6zA Crystal structure of a glutamyl-tRNA synthetase glurs from burkholderia thailandensis bound to l-glutamate (see paper)
39% identity, 71% coverage: 6:343/477 of query aligns to 3:319/380 of 4g6zA
6brlA Crystal structure of a glutamate tRNA ligase from elizabethkingia meningosepticum ccug26117 in complex with its amino acid (see paper)
29% identity, 96% coverage: 6:465/477 of query aligns to 3:497/502 of 6brlA
P27305 Glutamyl-Q tRNA(Asp) synthetase; Glu-Q-RSs; EC 6.1.1.- from Escherichia coli (strain K12) (see paper)
33% identity, 54% coverage: 1:258/477 of query aligns to 11:243/308 of P27305
- E55 (= E45) binding L-glutamate
- Y182 (= Y197) binding L-glutamate
- R200 (= R215) binding L-glutamate
4a91A Crystal structure of the glutamyl-queuosine trnaasp synthetase from e. Coli complexed with l-glutamate (see paper)
34% identity, 52% coverage: 9:257/477 of query aligns to 7:230/290 of 4a91A
- active site: S11 (= S13), K229 (= K256)
- binding glutamic acid: R7 (= R9), A9 (= A11), S11 (= S13), E43 (= E45), Y170 (= Y197), R188 (= R215), L192 (≠ H219)
- binding zinc ion: C99 (= C109), C101 (= C111), Y113 (= Y132), C117 (= C136)
3aiiA Archaeal non-discriminating glutamyl-tRNA synthetase from methanothermobacter thermautotrophicus (see paper)
29% identity, 58% coverage: 6:284/477 of query aligns to 11:278/455 of 3aiiA
P46655 Glutamate--tRNA ligase, cytoplasmic; Glutamyl-tRNA synthetase; (c)ERS; GluRS; P85; EC 6.1.1.17 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
24% identity, 48% coverage: 6:235/477 of query aligns to 202:420/708 of P46655
Sites not aligning to the query:
- 148 R→A: Abolishes interaction with ARC1.
Query Sequence
>WP_011812925.1 NCBI__GCF_000015585.1:WP_011812925.1
MNEMTVKTRFAPSPTGALHLGNLRTALFNALLAYGHGGRFVLRIEDTDRERYSPEATRSL
MGDLRWLGLDWQEGPEVGGPAPPYHQFERGFLYDAYYRQLEAEGWAYPCFCSERELEMAR
RAQRSAGQPPRYPGTCARLTAEEVARKRAEGMQPALRFRVPSGDEVRFEDRIHGEQRFRT
DDIGDFIIRRADGTAAYLFTNAVDDALMGITDVLRGDDHLTNTPRQLLILQALGLAAPRY
GHMGLITGNDGAPLSKRNGSRSVGELARDGYLPEAMLNYLARVGHTCESEALLDLAGLAE
GFDPARISTAPSRFDPDHLRHWQEEAVHGSSVRRLRPWMGIDALVPPGYAEDLVNAVRGN
VRFPAEARDWAKRVFAGPPELDADAEAAIVSAGAEFFEAAAGAAEEGYTRFKGLTEAVKE
RTGVKGKQLFMPLRAALTGRCSGPELQPVVDLMGGERVVERLRRAATLAAEQGAQGE
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory