SitesBLAST
Comparing WP_011812941.1 NCBI__GCF_000015585.1:WP_011812941.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3aexA Catalytic intermediate analogue of threonine synthase from thermus thermophilus hb8 (see paper)
54% identity, 91% coverage: 10:353/379 of query aligns to 5:327/351 of 3aexA
- active site: K61 (= K66), T85 (= T90), P212 (= P238), G216 (= G242), Q218 (= Q244), A240 (= A266), T317 (= T343)
- binding (3E)-4-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}-2-oxobut-3-enoic acid: K61 (= K66), S84 (= S89), T85 (= T90), N87 (= N92), T88 (= T93), V186 (= V191), G187 (= G192), N188 (= N193), A189 (= A194), G190 (= G195), N191 (= N196), A240 (= A266), I241 (= I267), E287 (= E313), T317 (= T343)
- binding phosphate ion: K61 (= K66), T88 (= T93), N154 (= N159), S155 (= S160), R160 (= R165), N188 (= N193)
1v7cA Crystal structure of threonine synthase from thermus thermophilus hb8 in complex with a substrate analogue (see paper)
54% identity, 91% coverage: 10:353/379 of query aligns to 5:327/351 of 1v7cA
- active site: K61 (= K66), T85 (= T90), P212 (= P238), G216 (= G242), Q218 (= Q244), A240 (= A266), T317 (= T343)
- binding (2e)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]-5-phosphonopent-2-enoic acid: K61 (= K66), S84 (= S89), T85 (= T90), N87 (= N92), T88 (= T93), F134 (= F139), N154 (= N159), S155 (= S160), R160 (= R165), V186 (= V191), G187 (= G192), N188 (= N193), A189 (= A194), G190 (= G195), N191 (= N196), A240 (= A266), I241 (= I267), E287 (= E313), T317 (= T343)
1uimA Crystal structure of threonine synthase from thermus thermophilus hb8, orthorhombic crystal form (see paper)
54% identity, 91% coverage: 10:353/379 of query aligns to 5:327/350 of 1uimA
- active site: K61 (= K66), T85 (= T90), P212 (= P238), G216 (= G242), Q218 (= Q244), A240 (= A266), T317 (= T343)
- binding pyridoxal-5'-phosphate: F60 (= F65), K61 (= K66), N87 (= N92), G187 (= G192), N188 (= N193), A189 (= A194), G190 (= G195), N191 (= N196), A240 (= A266), E287 (= E313), T317 (= T343), G318 (= G344)
A0R220 Threonine synthase; TS; EC 4.2.3.1 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
56% identity, 92% coverage: 7:353/379 of query aligns to 12:336/360 of A0R220
- K151 (≠ E148) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
3aeyA Apo form of threonine synthase from thermus thermophilus hb8 (see paper)
54% identity, 91% coverage: 10:353/379 of query aligns to 4:326/350 of 3aeyA
- active site: K60 (= K66), T84 (= T90), P211 (= P238), G215 (= G242), Q217 (= Q244), A239 (= A266), T316 (= T343)
- binding sulfate ion: K60 (= K66), K60 (= K66), G85 (= G91), N86 (= N92), T87 (= T93), T87 (= T93), S154 (= S160), R159 (= R165), N187 (= N193), R228 (≠ G255), V230 (= V257), E231 (≠ A258), R232 (≠ E259), A239 (= A266)
2zsjA Crystal structure of threonine synthase from aquifex aeolicus vf5
50% identity, 98% coverage: 6:375/379 of query aligns to 1:348/350 of 2zsjA
- active site: K61 (= K66), T85 (= T90), Q218 (= Q244), A222 (≠ S248), A240 (= A266), T317 (= T343)
- binding pyridoxal-5'-phosphate: F60 (= F65), K61 (= K66), N87 (= N92), V186 (= V191), G187 (= G192), N188 (= N193), A189 (= A194), G190 (= G195), N191 (= N196), A240 (= A266), T317 (= T343), G318 (= G344)
P9WG59 Threonine synthase; TS; EC 4.2.3.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
56% identity, 92% coverage: 7:353/379 of query aligns to 12:336/360 of P9WG59
- K69 (= K66) modified: N6-(pyridoxal phosphate)lysine
- N95 (= N92) binding pyridoxal 5'-phosphate
- K151 (≠ E148) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
- GNAGN 196:200 (= GNAGN 192:196) binding pyridoxal 5'-phosphate
- T326 (= T343) binding pyridoxal 5'-phosphate
2d1fA Structure of mycobacterium tuberculosis threonine synthase (see paper)
56% identity, 92% coverage: 7:353/379 of query aligns to 3:327/349 of 2d1fA
- active site: K60 (= K66), T84 (= T90), D209 (≠ E235), R213 (= R239), L215 (≠ V241), A240 (= A266), T317 (= T343)
- binding pyridoxal-5'-phosphate: F59 (= F65), K60 (= K66), N86 (= N92), V186 (= V191), G187 (= G192), N188 (= N193), A189 (= A194), G190 (= G195), N191 (= N196), A240 (= A266), T317 (= T343)
6nmxA Threonine synthase from bacillus subtilis atcc 6633 with plp and appa (see paper)
52% identity, 92% coverage: 7:353/379 of query aligns to 3:325/350 of 6nmxA
- active site: K60 (= K66), T84 (= T90), E216 (≠ Q244), S220 (= S248), A238 (= A266), T315 (= T343)
- binding (2E,3Z)-2-{[(Z)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4(1H)-ylidene}methyl]imino}-5-phosphonopent-3-enoic acid: K60 (= K66), S83 (= S89), T84 (= T90), N86 (= N92), T87 (= T93), F133 (= F139), N153 (= N159), S154 (= S160), R159 (= R165), V185 (= V191), G186 (= G192), N187 (= N193), A188 (= A194), G189 (= G195), N190 (= N196), A238 (= A266), I239 (= I267), E285 (= E313), T315 (= T343)
6cgqB Threonine synthase from bacillus subtilis atcc 6633 with plp and plp- ala (see paper)
52% identity, 92% coverage: 7:353/379 of query aligns to 1:323/345 of 6cgqB
- active site: K58 (= K66), T82 (= T90), E214 (≠ Q244), S218 (= S248), A236 (= A266), T313 (= T343)
- binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-alanine: K58 (= K66), S81 (= S89), T82 (= T90), N84 (= N92), T85 (= T93), V183 (= V191), G184 (= G192), N185 (= N193), A186 (= A194), N188 (= N196), A236 (= A266), I237 (= I267), E283 (= E313), T313 (= T343)
- binding phosphate ion: K58 (= K66), T85 (= T93), N151 (= N159), S152 (= S160), R157 (= R165), N185 (= N193)
6cgqA Threonine synthase from bacillus subtilis atcc 6633 with plp and plp- ala (see paper)
51% identity, 91% coverage: 9:353/379 of query aligns to 1:315/339 of 6cgqA
- active site: K56 (= K66), T80 (= T90), E206 (≠ Q244), S210 (= S248), A228 (= A266), T305 (= T343)
- binding pyridoxal-5'-phosphate: F55 (= F65), K56 (= K66), N82 (= N92), V175 (= V191), G176 (= G192), N177 (= N193), A178 (= A194), G179 (= G195), N180 (= N196), A228 (= A266), E275 (= E313), T305 (= T343), G306 (= G344)
2c2bA Crystallographic structure of arabidopsis thaliana threonine synthase complexed with pyridoxal phosphate and s-adenosylmethionine (see paper)
34% identity, 99% coverage: 5:379/379 of query aligns to 67:444/444 of 2c2bA
- binding pyridoxal-5'-phosphate: F127 (= F65), K128 (= K66), D159 (≠ N92), G259 (≠ V191), G260 (= G192), N261 (= N193), L262 (≠ A194), G263 (= G195), N264 (= N196), A321 (= A266), H369 (≠ A315), T397 (= T343)
- binding s-adenosylmethionine: P67 (= P5), G69 (≠ Y7), S90 (≠ G28), F92 (≠ G30), N97 (≠ P35), L98 (= L36), W100 (≠ Q38), W115 (≠ Y53), W115 (≠ Y53), Q246 (≠ E179), F247 (≠ L180)
Sites not aligning to the query:
Q9S7B5 Threonine synthase 1, chloroplastic; Protein METHIONINE OVER-ACCUMULATOR 2; EC 4.2.3.1 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
34% identity, 99% coverage: 5:379/379 of query aligns to 142:519/526 of Q9S7B5
- N172 (≠ P35) binding S-adenosyl-L-methionine
- L173 (= L36) binding S-adenosyl-L-methionine
- K181 (≠ A44) binding in monomer B; binding in monomer A
- N187 (≠ V50) binding in monomer B
- L205 (≠ R68) mutation to R: In mto2-1; causes a strong decrease in the concentration of soluble threonine and over-accumulation of methionine.
2c2gA Crystal structure of threonine synthase from arabidopsis thaliana in complex with its cofactor pyridoxal phosphate (see paper)
33% identity, 99% coverage: 5:379/379 of query aligns to 85:446/448 of 2c2gA
7mfjBBB Beta-cyanoalanine synthase (see paper)
27% identity, 84% coverage: 32:348/379 of query aligns to 22:318/319 of 7mfjBBB
3pc2A Full length structure of cystathionine beta-synthase from drosophila (see paper)
30% identity, 51% coverage: 32:224/379 of query aligns to 46:240/500 of 3pc2A
- active site: K80 (= K66)
- binding protoporphyrin ix containing fe: A187 (≠ Q167), P190 (≠ K170), L191 (≠ T171), Y194 (≠ F174), R227 (= R210)
- binding pyridoxal-5'-phosphate: K80 (= K66), N110 (= N92), A216 (≠ V191), G217 (= G192), T218 (≠ N193), A219 (= A194), T221 (≠ N196)
Sites not aligning to the query:
- active site: 310
- binding protoporphyrin ix containing fe: 12, 13, 14, 15, 16, 24, 25, 26
- binding pyridoxal-5'-phosphate: 266, 310, 336, 337
3pc4A Full length structure of cystathionine beta-synthase from drosophila in complex with serine (see paper)
30% identity, 51% coverage: 32:224/379 of query aligns to 48:242/504 of 3pc4A
- active site: K82 (= K66)
- binding protoporphyrin ix containing fe: A189 (≠ Q167), P192 (≠ K170), L193 (≠ T171), Y196 (≠ F174), R229 (= R210)
- binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine: K82 (= K66), T109 (≠ S89), S110 (≠ T90), N112 (= N92), T113 (= T93), Q185 (≠ P163), A218 (≠ V191), G219 (= G192), T220 (≠ N193), A221 (= A194), T223 (≠ N196)
Sites not aligning to the query:
- active site: 312
- binding protoporphyrin ix containing fe: 14, 15, 16, 17, 18, 26, 27, 28, 276
- binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine: 268, 269, 271, 312, 338, 339
3pc3A Full length structure of cystathionine beta-synthase from drosophila in complex with aminoacrylate (see paper)
30% identity, 51% coverage: 32:224/379 of query aligns to 48:242/504 of 3pc3A
- active site: K82 (= K66)
- binding protoporphyrin ix containing fe: A189 (≠ Q167), P192 (≠ K170), L193 (≠ T171), Y196 (≠ F174), R229 (= R210)
- binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: K82 (= K66), T109 (≠ S89), S110 (≠ T90), N112 (= N92), T113 (= T93), Q185 (≠ P163), A218 (≠ V191), G219 (= G192), T220 (≠ N193), A221 (= A194), T223 (≠ N196)
Sites not aligning to the query:
- active site: 312
- binding protoporphyrin ix containing fe: 14, 15, 16, 17, 18, 26, 27, 28
- binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: 268, 269, 312, 338, 339
6xo2A Structural characterization of beta cyanoalanine synthase from tetranychus urticae (two-spotted spider mite) (see paper)
26% identity, 84% coverage: 32:350/379 of query aligns to 23:294/294 of 6xo2A
- binding pyridoxal-5'-phosphate: L53 (≠ F65), K54 (= K66), N84 (= N92), C195 (= C223), G196 (= G224), T197 (vs. gap), G198 (= G225), C200 (= C227), I226 (≠ Q273), T259 (≠ A315), C287 (≠ T343), D288 (≠ G344)
4pcuA Crystal structure of delta516-525 e201s human cystathionine beta- synthase with adomet (see paper)
26% identity, 78% coverage: 32:325/379 of query aligns to 43:315/486 of 4pcuA
- active site: K77 (= K66), S105 (≠ T90), D237 (≠ Q244), S305 (≠ A315)
- binding protoporphyrin ix containing fe: A182 (≠ S160), P185 (= P163), L186 (= L166), Y189 (≠ Q169), R222 (≠ E214), T269 (≠ A280)
- binding pyridoxal-5'-phosphate: K77 (= K66), N107 (= N92), G212 (= G192), T213 (≠ N193), G214 (≠ A194), T216 (≠ N196), G261 (≠ P272), S305 (≠ A315)
Sites not aligning to the query:
- binding protoporphyrin ix containing fe: 8, 9, 10, 11, 12, 20, 21, 22, 23
- binding pyridoxal-5'-phosphate: 331, 332
- binding s-adenosylmethionine: 376, 396, 397, 398, 399, 476, 478, 479
Query Sequence
>WP_011812941.1 NCBI__GCF_000015585.1:WP_011812941.1
MPFRPRYTGLIAKYLDRLPISDDARILGLGEGNTPLIQLTRIPAELGRDVDLYVKFEGLN
PTGSFKDRGMTMAVTKAVEQGAKAIICASTGNTSASAAAYAARAGISCFVLIPDGKIAMG
KLAQAIMHGAQVLQIRGNFDAGMRLVKELAEHAPLTIVNSINPYRLQGQKTAAFEIIEEL
ERAPDYHCLPVGNAGNITAHWIGYSECAGRTGDEQLTAACAFCGGQCRYASALVERRPRM
VGYQASGSAPFLRGGPVAEPETVATAIRIGDPQSWDYAQAVREESGGWFDELSDEEILQA
QRMLADHEGVFCEPASATSVAGAMRDIRSGRIPEGSTVVCTLTGHGLKDPDVASAQAGDA
VQTVDADYQAVREAILKRL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory