SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011813685.1 NCBI__GCF_000015585.1:WP_011813685.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 5 hits to proteins with known functional sites (download)

O53289 Phosphoserine phosphatase SerB2; PSP; PSPase; O-phosphoserine phosphohydrolase; Protein-serine/threonine phosphatase; EC 3.1.3.3; EC 3.1.3.16 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
25% identity, 88% coverage: 5:203/227 of query aligns to 183:365/409 of O53289

query
sites
O53289

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D185 (= D7) mutation to G: Completely abolishes enzymatic activity.; mutation to N: Completely abolishes enzymatic activity.
V186 (≠ L8) mutation to Q: Decreases enzymatic activity by 50%.
D187 (= D9) mutation to N: Decreases enzymatic activity by 15%.
S188 (≠ N10) mutation to A: No effect on enzymatic activity.
S273 (≠ T111) mutation to A: Completely abolishes enzymatic activity (PubMed:25521849). Decreases enzymatic activity by 60% (PubMed:25037224).
K318 (= K156) mutation to A: Decreases enzymatic activity by 50%.; mutation to E: Completely abolishes enzymatic activity.
D341 (= D179) mutation to G: Decreases enzymatic activity by 80%.; mutation to N: Decreases enzymatic activity by 85%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-345.
D345 (= D183) mutation to N: Decreases enzymatic activity by 55%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-341.

Sites not aligning to the query:

18 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.
108 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.

8a21A Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with phenylimidazole (see paper)
27% identity, 88% coverage: 5:203/227 of query aligns to 181:363/396 of 8a21A

query
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8a21A

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binding magnesium ion: D183 (= D7), D185 (= D9), D339 (= D179)
binding 4-phenyl-1h-imidazole: D185 (= D9), E192 (≠ D16), V193 (≠ S17), I194 (≠ D18), T211 (= T39), M215 (≠ F43), F221 (≠ I54), R228 (≠ A61), G273 (≠ T113)

Sites not aligning to the query:

binding 4-phenyl-1h-imidazole: 13, 18, 37

8a1zA Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with 1-(2,4-dichlorophenyl)-3-hydroxyurea (see paper)
27% identity, 88% coverage: 5:203/227 of query aligns to 181:363/396 of 8a1zA

query
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8a1zA

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binding 1-(2,4-dichlorophenyl)-3-oxidanyl-urea: D185 (= D9), E192 (≠ D16), M215 (≠ F43), F221 (≠ I54), L225 (= L58), R228 (≠ A61), G272 (≠ A112), F274 (≠ N114), D339 (= D179)
binding magnesium ion: D183 (= D7), D185 (= D9), D339 (= D179)

A0QJI1 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Mycobacterium avium (strain 104) (see paper)
27% identity, 88% coverage: 5:203/227 of query aligns to 185:367/411 of A0QJI1

query
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A0QJI1

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D187 (= D7) binding Mg(2+)
D189 (= D9) binding Mg(2+)
D343 (= D179) binding Mg(2+)

5jlpA Crystal structure of mycobacterium avium serb2 in complex with serine at act domain
27% identity, 88% coverage: 5:203/227 of query aligns to 181:363/396 of 5jlpA

query
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5jlpA

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active site: D183 (= D7), V184 (≠ L8), D185 (= D9), G272 (≠ A112), K316 (= K156), D343 (= D183)
binding magnesium ion: D183 (= D7), D339 (= D179)

Sites not aligning to the query:

binding serine: 13, 14

Query Sequence

>WP_011813685.1 NCBI__GCF_000015585.1:WP_011813685.1
MSVALFDLDNTLIAGDSDSLWGEYLVACGAVDSEAFAETHRRFHQDYIEGRLDIDAFLAF
ALSPLAQHPEEQLHAWRADFTRDWIEPLVLPAAEALLEEHRRAGHHLAIVTATNRFVTAP
IAERLDVGTLLATEPERRDGRYTGRHTGTPTFREGKIRVVEAWLREHGLEQAEKWFYSDS
LNDLPLLRHVEHPVAVDPDDTLRAEARRNGWPVLTLRQAETPECCSA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory