SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011813799.1 NCBI__GCF_000015585.1:WP_011813799.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P12282 Molybdopterin-synthase adenylyltransferase; MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase; Sulfur carrier protein MoaD adenylyltransferase; EC 2.7.7.80 from Escherichia coli (strain K12) (see 2 papers)
48% identity, 99% coverage: 1:245/247 of query aligns to 4:249/249 of P12282

query
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P12282

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R14 (= R11) mutation R->A,K: No effect.; mutation to A: No activity; when associated with A-73.
C44 (≠ S41) mutation to A: No effect.
R73 (= R70) mutation to A: No effect. No activity; when associated with A-14.; mutation to K: Substantially reduced activity.
C128 (≠ A125) mutation to A: No effect.; mutation to Y: No activity.
D130 (= D127) mutation to A: No activity.; mutation to E: Substantially reduced activity.
C142 (= C139) mutation to A: No effect.
C172 (= C168) mutation to A: No zinc bound and no enzyme activity.
C175 (= C171) mutation to A: No zinc bound and no enzyme activity.
C187 (= C183) mutation to A: No effect.
C231 (≠ M227) mutation to A: No effect.
C244 (= C240) mutation to A: No zinc bound and almost no enzyme activity.
C247 (= C243) mutation to A: No zinc bound and almost no enzyme activity.

1jwbB Structure of the covalent acyl-adenylate form of the moeb-moad protein complex (see paper)
47% identity, 98% coverage: 1:243/247 of query aligns to 3:239/240 of 1jwbB

query
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1jwbB

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active site: R13 (= R11), D129 (= D127)
binding adenosine monophosphate: G37 (= G35), G39 (= G37), G40 (= G38), D61 (= D59), F62 (≠ D60), K85 (= K83), L108 (≠ R106), C127 (≠ A125), T128 (= T126), D129 (= D127), N130 (= N128), V133 (≠ T131)
binding zinc ion: C171 (= C168), C236 (= C240), C239 (= C243)

1jw9B Structure of the native moeb-moad protein complex (see paper)
47% identity, 98% coverage: 1:243/247 of query aligns to 3:239/240 of 1jw9B

query
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1jw9B

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active site: R13 (= R11), D129 (= D127)
binding zinc ion: C171 (= C168), C236 (= C240), C239 (= C243)

1zfnA Structural analysis of escherichia coli thif (see paper)
47% identity, 98% coverage: 1:243/247 of query aligns to 1:243/244 of 1zfnA

query
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1zfnA

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active site: R11 (= R11), D127 (= D127)
binding adenosine-5'-triphosphate: I34 (≠ V34), G35 (= G35), G37 (= G37), G38 (= G38), D59 (= D59), R70 (= R70), Q71 (= Q71), K83 (= K83), T126 (= T126), D127 (= D127), T131 (= T131)
binding zinc ion: C169 (= C168), C172 (= C171), C240 (= C240), C243 (= C243)

P30138 Sulfur carrier protein ThiS adenylyltransferase; EC 2.7.7.73 from Escherichia coli (strain K12) (see 3 papers)
47% identity, 98% coverage: 1:243/247 of query aligns to 1:243/251 of P30138

query
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P30138

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Q

A

A

V

L

E

E

A

A

I

I

K

K

I

L

L

L

T

S

G

G

A

I

G

E

T

T

P

P

L

-

F

A

G

G

R

E

L

L

L

R

V

L

V

F

D

D

A

G

L

K

R

S

M

S

R

Q

L

W

R

R

T

S

L

L

T

A

V

L

N

R

G

R

D

A

P

S

H

G

C
|
C

P

P

V

V

C
|
C

C169 (= C168) binding Zn(2+)
C172 (= C171) binding Zn(2+)
W174 (≠ Y173) mutation to A: No adenylation of ThiS.
C184 (= C183) mutation to S: No cross-link formed with ThiS. No effect on ThiS thiocarboxylate formation in vitro. Does not support growth.
C240 (= C240) binding Zn(2+)
C243 (= C243) binding Zn(2+)

D4GSF3 SAMP-activating enzyme E1; Ubiquitin-like activating enzyme of archaea; Ubl-activating enzyme; EC 2.7.7.- from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see paper)
48% identity, 98% coverage: 5:245/247 of query aligns to 9:250/270 of D4GSF3

query
sites
D4GSF3

Q

Q

L

L

M

D

R

R

Y

Y

S

S

R

R

Q

H

I

I

M

I

L

M

P

D

E

E

L

V

D

G

I

P

T

E

G

G

Q

Q

E

G

R

R

L

L

A

L

A

S

S

S

R

R

A

V

L

V

I

V

V

V

G

G

A

A

G

G

L

L

G

G

S

A

P

P

V

A

A

I

L

Q

Y

Y

L

L

G

A

A

A

A

V

G

G

V

V

G

G

E

E

L

L

R

V

I

V

A

V

D

D

V

V

D

E

L

R

S

S

N

N

L

L

Q

Q

R

R

Q

Q

I

V

A

V

H

H

R

C

Q

D

D

D

A

D

L

V

G

G

Q

T

P

P

K

K

A

A

A

E

S

S

A

A

T

A

R

A

A

F

V

V

T

R

A

G

R

L

N

N

P

P

E

D

I

V

T

S

V

V

T

E

P

P

L

V

E

E

E

A

R

R

L

V

D

D

G

K

E

S

R

N

L

V

G

H

A

E

E

V

V

V

G

A

A

G

A

S

D

D

V

V

I

V

D

D

A

A

T

S

D

D

N

N

F

F

A

P

T

T

R

R

F

Y

A

L

L

L

N

N

A

D

A

V

C

C

V

R

A

F

A

E

H

G

R

I

P

P

L

L

V

V

S

H

A

G

A

A

V

I

I

Y

R

K

W

F

E

E

L

G

Q

Q

V

A

S

T

A

T

F

L

R

V

P

P

G

D

G

G

R

-

P

P

C

C

Y

Y

R

R

C

C

I

L

Y

F

A

P

E

E

G

A

D

P

E

E

P

P

Q

G

L

T

T

V

-

P

-

D

C
|
C

S

A

E

T

S

T

G

G

V

V

L

L

G

G

P

V

L

L

P

P

G

G

V

T

A

V

G

G

S

C

L

I

E

Q

A

A

V

T

E

E

A

A

I

M

K

K

I

L

L

L

T

L

G

D

A

E

G

G

T

E

P

A

L

L

F

D

G

G

R

R

L

L

V

F

V

Y

D

D

A

A

L

M

R

D

M

M

R

T

L

F

R

E

T

T

L

V

T

P

V

Y

N

R

G

T

D

N

P

P

H

D

C

C

P

P

V

V

C

C

A

G

E

E

C188 (= C183) mutation to S: Loss of activity since this mutant is not able to complement a ubaA deletion in trans to restore sampylation and tRNA thiolation.

1zud3 Structure of this-thif protein complex (see paper)
46% identity, 98% coverage: 1:243/247 of query aligns to 1:238/240 of 1zud3

query
sites
1zud3

M

M

N

N

D

D

E

R

Q

D

L

F

M

M

R

R

Y

Y

S

S

R
|
R

Q

Q

I

I

M
x
L

L

L

P
x
D

E

D

L

I

D

A

I

L

T

D

G

G

Q

Q

E

Q

R

K

L

L

A

L

A

D

S

S

R

Q

A

V

L

L

I

I

V

I

G

G

A

L

G

G

L

L

G

G

S

T

P

P

V

A

A

A

L

L

Y

Y

L

L

G

A

A

G

A

A

G

G

V

V

G

G

E

T

L

L

R

V

I

L

A

A

D

D

V

D

V

V

D

H

L

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

I

I

A

L

H

F

R

T

Q

T

D

E

A

D

L

I

G

D

Q

R

P

P

K

K

A

S

A

Q

S

V

A

S

T

Q

R

Q

A

R

V

L

T

T

A

Q

R

L

N

N

P

P

E

D

I

I

T

Q

V

L

T

T

P

A

L

L

E

Q

R

R

L

L

D

T

G

G

E

E

R

A

L

L

G

K

A

D

E

A

V

V

G

A

A

R

A

A

D

D

V

V

I

L

D

D

A

C

T

T

D
|
D

N

N

F

M

A

A

T

T

R

R

F

Q

A

E

L

I

N

N

A

A

C

C

V

V

A

A

A

L

H

N

R

T

P

P

L

L

V

I

S

T

A

A

A

S

V

A

I

V

R

G

W

F

E

G

L

G

Q

Q

V

L

S

M

A

V

F

L

R

T

-

P

P

P

G

W

G

E

R

Q

P

G

C
|
C

Y

Y

R

R

C

C

I

L

Y

W

A

P

E

D

G

N

D

Q

E

E

P

P

Q

-

L

-

T

-

C

-

S

-

E

T

S

A

G

G

V

V

L

V

G

G

P

P

L

V

P

V

G

G

V

V

A

M

G

G

S

T

L

L

E

Q

A

A

V

L

E

E

A

A

I

I

K

K

I

L

L

L

T

S

G

G

A

I

G

E

T

T

P

P

L

-

F

A

G

G

R

E

L

L

L

R

V

L

V

F

D

D

A

G

L

K

R

S

M

S

R

Q

L

W

R

R

T

S

L

L

T

A

V

L

N

R

G

R

D

A

P

S

H

G

C
|
C

P

P

V

V

C
|
C

active site: R11 (= R11), D127 (= D127)
binding calcium ion: L14 (≠ M14), D16 (≠ P16)
binding zinc ion: C169 (= C168), C235 (= C240), C238 (= C243)

1jwaB Structure of the atp-bound moeb-moad protein complex (see paper)
44% identity, 95% coverage: 1:234/247 of query aligns to 3:215/217 of 1jwaB

query
sites
1jwaB

M

L

N

S

D

D

E

Q

Q

E

L

M

M

L

R

R

Y

Y

S

N

R
|
R

Q

Q

I

I

M

I

L

L

P

R

E

G

L

F

D

D

I

F

T

D

G

G

Q

Q

E

E

R

A

L

L

A

K

A

D

S

S

R

R

A

V

L

L

I

I

V

V

G

G

A

L

G
|
G

L

L

G

G

S

C

P

A

V

A

A

S

L

Q

Y

Y

L

L

G

A

A

S

A

A

G

G

V

V

G

G

E

N

L

L

R

T

I

L

A

L

D
|
D

D
x
F

D

D

V

T

V

V

D

S

L

L

S

S

N

N

L

L

Q

Q

R
|
R

Q

Q

I

T

A

L

H

H

R

S

Q

D

D

A

A

T

L

V

G

G

Q

Q

P

P

K
|
K

A

V

A

E

S

S

A

A

T

R

R

D

A

A

V

L

T

T

A

R

R

I

N

N

P

P

E

H

I

I

T

A

V

I

T

T

P

P

L

V

E

N

E

A

R
x
L

L

L

D

D

G

D

E

A

R

E

L

L

G

A

A

A

E

L

V

I

G

A

A

E

A

H

D

D

V

L

V

V

I

L

D

D

A

C

T

T

D
|
D

N
|
N

F

V

A

A

T
x
V

R

R

F

N

A

Q

L

L

N

N

A

A

A

G

C

C

V

F

A

A

H

K

R

V

P

P

L

L

V

V

S

S

A

G

A

A

V

A

I

I

R

R

W

M

E

E

L

G

Q

Q

V

I

S

T

A

V

F

F

R

-

P

-

G

-

R

-

P

-

C

-

Y

-

R

-

C

-

I

-

Y

-

A

-

E

-

G

-

D

-

E

-

P

-

Q

-

L

-

T

-

C

T

S

Y

E

E

S

A

G

G

V

V

L

M

G

A

P

P

L

L

P

I

G

G

V

V

A

I

G

G

S

S

L

L

E

Q

A

A

V

M

E

E

A

A

I

I

K

K

I

M

L

L

T

A

G

G

A

Y

G

G

T

K

P

P

L

A

F

S

G

G

R

K

L

I

L

V

V

M

V

Y

D

D

A

A

L

M

R

T

M

C

R

Q

L

F

R

R

T

E

L

M

T

K

V

L

active site: R13 (= R11), D129 (= D127)
binding adenosine-5'-triphosphate: G39 (= G37), G40 (= G38), D61 (= D59), F62 (≠ D60), R72 (= R70), K85 (= K83), L108 (≠ R106), D129 (= D127), N130 (= N128), V133 (≠ T131)

Q9VLJ8 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Ubiquitin activating enzyme 4; EC 2.7.7.80; EC 2.8.1.11 from Drosophila melanogaster (Fruit fly) (see paper)
39% identity, 100% coverage: 1:247/247 of query aligns to 64:313/453 of Q9VLJ8

query
sites
Q9VLJ8

M

L

N

T

D

N

E

D

Q

D

L

I

M

A

R

R

Y

Y

S

S

R

R

Q

Q

I

L

M

I

L

L

P

P

E

D

L

F

D

G

I

V

T

Q

G

G

Q

Q

E

L

R

K

L

L

A

K

A

N

S

S

R

S

A

V

L

L

I

I

V

V

G

G

A

L

G

G

L

L

G

G

S

C

P

P

V

A

A

A

L

Q

Y

Y

L

L

G

A

A

A

G

G

V

C

G

G

E

H

L

L

R

G

I

L

A

V

D

D

D

Y

D

D

V

E

V

V

D

E

L

R

S

S

N

N

L

F

Q

H

R

R

Q

Q

I

I

A

L

H

H

R

S

Q

E

D

D

A

R

L

C

G

G

Q

M

P

S

K

K

A

A

A

E

S

S

A

A

T

R

R

I

A

A

V

L

T

L

A

E

R

L

N

N

P

P

E

H

I

C

T

E

V

I

T

Q

P

C

L

H

E

S

E

R

R

M

L

L

D

Y

G

P

E

H

R

N

L

A

G

M

A

H

E

I

V

I

G

R

A

G

A

Y

D

D

V

V

I

L

D

D

A

C

T

T

D

D

N

N

F

V

A

P

T

T

R

R

F

Y

A

L

L

L

N

S

A

D

A

A

C

C

V

V

A

M

A

L

H

S

R

K

P

P

L

L

V

V

S

S

A

G

A

S

V

A

I

L

R

K

W

M

E

D

L

G

Q

Q

V

L

S

T

A

V

F

Y

R

N

P

Y

G

A

G

N

R

G

P

P

C

C

Y

Y

R

R

C

C

I

I

Y

F

A

P

E

V

G

P

D

P

E

P

P

P

Q

E

-

A

-

V

L

T

T

N

C

C

S

G

E

D

S

G

G

G

V

V

L

L

G

G

P

A

L

V

P

T

G

G

V

T

A

I

G

G

S

A

L

M

E

Q

A

A

V

L

E

E

A

A

I

I

K

K

I

V

L

I

T

V

G

G

A

M

G

G

T

D

P

V

L

L

F

A

G

G

R

R

L

L

V

I

V

F

D

D

A

G

L

G

R

S

M

G

R

V

L

F

R

R

T

N

L

I

T

R

V

I

N

R

G

S

D

K

-

R

P

P

H

N

C

C

P

H

V

M

C

C

A

S

E

A

A

Q

P

P

Sites not aligning to the query:

62 modified: Phosphothreonine

Q72J02 Sulfur carrier protein adenylyltransferase; E1-like protein TtuC; Sulfur carrier protein MoaD adenylyltransferase; Sulfur carrier protein ThiS adenylyltransferase; Sulfur carrier protein TtuB adenylyltransferase; tRNA two-thiouridine-synthesizing protein C; EC 2.7.7.80; EC 2.7.7.73; EC 2.7.7.- from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
43% identity, 100% coverage: 2:247/247 of query aligns to 4:256/271 of Q72J02

query
sites
Q72J02

N

T

D

K

E

E

Q

E

L

L

M

D

R

R

Y

Y

S

H

R

R

Q

Q

I

M

M

I

L

L

P

P

E

Q

L

V

D

G

I

P

T

E

G

G

Q

Q

E

E

R

R

L

L

A

K

A

R

S

A

R

S

A

V

L

V

I

V

V

V

G

G

A

A

G

G

L

L

G

G

S

V

P

P

V

V

A

L

L

Q

Y

Y

L

L

G

V

A

A

G

G

V

V

G

G

E

R

L

V

R

G

I

V

A

V

D

E

D

M

D

D

V

R

V

V

D

E

L

V

S

S

N

N

L

L

Q

H

R

R

Q

Q

I

V

A

L

H

Y

R

T

Q

T

D

E

A

D

L

V

G

G

Q

E

P

P

K

K

A

A

A

L

S

V

A

A

T

Q

R

K

A

R

V

L

T

Q

A

A

R

L

N

N

P

P

E

L

I

V

T

R

V

V

T

E

P

A

L

Y

E

P

E

V

R

R

L

L

D

T

G

S

E

E

R

N

L

A

G

L

A

E

E

I

V

L

G

R

A

P

A

Y

D

D

V

L

V

V

I

V

D

D

A

A

T

S

D

D

N

N

F

F

A

P

T

T

R

R

F

Y

A

L

L

V

N

N

A

D

A

A

C

A

V

V

A

L

H

G

R

K

P

P

L

L

V

V

S

F

A

G

A

A

V

I

I

Y

R

Q

W

F

E

D

L

G

Q

Q

V

V

S

A

A

V

F

F

R

H

P

H

G

P

G

T

R

L

-

H

-

G

-

E

-

M

-

G

P

P

C

C

Y

Y

R

R

C

C

I

L

Y

F

-

P

-

K

-

P

A

P

E

P

G

G

D

A

E

V

P

P

Q

-

L

-

T

S

C
|
C

S

A

E

E

S

A

G

G

V

V

L

F

G

G

P

V

L

L

P

P

G

A

V

V

A

V

G

G

S

S

L

L

E

M

A

A

V

A

E

E

A

A

I

L

K

K

I

V

L

L

T

L

G

G

A

I

G

G

T

K

P

P

L

L

F

A

G

G

R

H

L

L

V

L

V

Y

D

D

A

A

L

L

R

E

M

A

R

S

L

F

R

R

T

K

L

L

T

T

V

V

N

R

G

R

D

N

P

P

H

R

C

C

P

P

V

V

C

C

A

G

E

D

A

E

P

P

C192 (= C183) modified: Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in TtuB); mutation to S: Not able to form a thioester complex with TtuB.

Sites not aligning to the query:

268 C→S: Still able to form a thioester complex with TtuB.

O95396 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; MPT synthase sulfurylase; EC 2.7.7.80; EC 2.8.1.11 from Homo sapiens (Human) (see 5 papers)
43% identity, 100% coverage: 1:247/247 of query aligns to 55:304/460 of O95396

query
sites
O95396

M

L

N

S

D

R

E

D

Q

E

L

I

M

L

R

R

Y

Y

S

S

R

R

Q

Q

I

L

M

V

L

L

P

P

E

E

L

L

D

G

I

V

T

H

G

G

Q

Q

E

L

R

R

L

L

A

G

A

T

S

A

R

C

A

V

L

L

I

I

V

V

G

G

A

C

G

G

L

L

G

G

S

C

P

P

V

L

A

A

L

Q

Y

Y

L

L

G

A

A

A

G

G

V

V

G

G

E

R

L

L

R

G

I

L

A

V

D

D

D

Y

D

D

V

V

D

E

L

M

S

S

N

N

L

L

Q

A

R

R

Q

Q

I

V

A

L

H

H

R

G

Q

E

D

A

A

L

L

A

G

G

Q

Q

P

A

K

K

A

A

A

F

S

S

A

A

T

A

R

A

A

S

V

L

T

R

A

R

R

L

N

N

P

S

E

A

I

V

T

E

V

C

T

V

P

P

L

Y

E
x
T

E
x
Q

R
x
A

L
|
L

D
x
T

G
x
P

E
x
A

R
x
T

L
x
A

G
x
L

A
x
D

E
x
L

V
|
V

G
x
R

A
x
R

A
x
Y

D
|
D

V
|
V

I
x
A

D
|
D

A
x
C

T
x
S

D
|
D

N
|
N

F
x
V

A
x
P

T
|
T

R
|
R

F
x
Y

A
x
L

L
x
V

N
|
N

A
x
D

A
|
A

C
|
C

V
|
V

A
x
L

A
|
A

H
x
G

R
|
R

P
|
P

L
|
L

V
|
V

S
|
S

A
|
A

A
x
S

V
x
A

I
x
L

R
|
R

W
x
F

E
|
E

L
x
G

Q
|
Q

V
x
I

S
x
T

A
x
V

F
x
Y

R
x
H

P
x
Y

G
x
D

G
|
G

R
x
G

P
|
P

C
|
C

Y
|
Y

R
|
R

C
|
C

I
|
I

Y
x
F

A
x
P

E
x
Q

G
x
P

D
x
P

-
x
P

-
x
A

E
|
E

P
x
T

Q
x
V

L
x
T

T
x
N

C
|
C

S

A

E

D

S

G

G

G

V

V

L

L

G

G

P

V

L

V

P

T

G

G

V

V

A

L

G

G

S

C

L

L

E

Q

A

A

V

L

E

E

A

V

I

L

K

K

I

I

L

A

T

A

G

G

A

L

G

G

T

P

P

S

L

Y

F

S

G

G

R

S

L

L

V

L

V

F

D

D

A

A

L

L

R

R

M

G

R

H

L

F

-

R

R

S

T

I

L

R

T

L

V

R

N

S

G

R

D

R

P

L

H

D

C

C

P

A

V

A

C

C

A

G

E

E

A

R

P

P

158:238 (vs. 104:182, 40% identical) Interaction with NFS1
C239 (= C183) mutation to A: Impairs sulfurtransferase activity.

Sites not aligning to the query:

316 modified: Disulfide link with 324; C→A: Does not affect sulfurtransferase activity.
324 modified: Disulfide link with 316; C→A: Does not affect sulfurtransferase activity.
365 C→A: Does not affect sulfurtransferase activity.
412 active site, Cysteine persulfide intermediate; for sulfurtransferase activity; modified: Cysteine persulfide; C→A: Abolishes sulfurtransferase activity.
413 K→R: Does not affect sulfurtransferase specificity and activity.
414 L→K: Does not affect sulfurtransferase specificity and activity.
415 G→A: Does not affect sulfurtransferase specificity and activity.
416 N→V: Does not affect sulfurtransferase specificity and activity.
417 D→R: Results in 470-fold increased activity.; D→T: Results in 90-fold increased activity.
458 P→G: Does not affect sulfurtransferase specificity and activity.
460 Y→A: Does not affect sulfurtransferase specificity and activity.

O59954 Adenylyltransferase and sulfurtransferase uba4; Common component for nitrate reductase and xanthine dehydrogenase protein F; Ubiquitin-like protein activator 4; EC 2.7.7.80; EC 2.8.1.11 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
37% identity, 99% coverage: 1:244/247 of query aligns to 61:319/482 of O59954

query
sites
O59954

M

L

N

H

D

G

E

E

Q

E

L

Y

M

R

R

R

Y

Y

S

G

R

R

Q

Q

I

M

M

I

L

V

P

P

E

Q

L

F

D

G

I

L

T

Q

G
|
G

Q

Q

E

L

R

K

L

L

A

R

A

D

S

A

R

K

A

V

L

L

I

I

V

V

G

G

A

A

G

G

L

L

G
|
G

S

C

P

P

V

A

A

A

L

L

Y

Y

L

L

G

A

A

G

A

A

G

G

V

V

G

G

E

T

L

I

R

G

I

L

A

V

D

D

D

G

D

D

V

T

V

V

D

E

L

A

S

S

N

N

L

L

Q

H

R
|
R

Q

Q

I

V

A

L

H

H

R

R

Q

S

D

R

A

N

L

V

G

G

Q

K

P

L

K

K

A

V

A

D

S

S

A

A

T

I

R

E

A

Y

V

L

T

R

A

E

R

L

N

N

P

P

E

H

I

P

T

T

V

Y

T

I

P

A

L

H

E

Q

E

A

R

H

L

L

D

T

G

P

E

R

R

E

L

A

G

P

A

D

E

I

V

F

G

K

A

D

A

Y

D

D

V

L

V

I

I

L

D

D

A
x
C

T

T

D

D

N

N

F

P

A

A

T

T

R

R

F

Y

A

L

L

I

N

S

A

D

A

T

C

A

V

V

A

L

H

G

R

K

P

P

L

L

V

V

S

S

A

A

A

S

V

A

I

L

R

R

W

T

E
|
E

L

G

Q

Q

V

L

S

M

A

V

F

L

R

N

-

N

-

P

-

Q

-

P

P

P

G

G

-

D

-

K

-

T

G

G

R

G

P

P

C

C

Y

Y

R

R

C

C

I

V

Y

F

A

P

E

K

G

P

D

P

E

P

P

A

Q

N

-

S

-

V

L

T

T

S

C

C

S

A

E

D

S

G

G

G

V

I

L

L

G

G

P

P

L

V

P

V

G
|
G

V

T

A

M

G

G

S

V

L

L

E

Q

A

A

V

S

E

E

A

A

I

I

K

K

I

V

L

L

T

T

G

S

A

A

G

G

T

E

P

S

L

V

F

E

G

A

-

T

-

P

R

S

L

L

V

I

V

F

D

S

A

A

L

Y

R

S

M

S

-

P

R

Q

L

F

R

R

T

T

L

I

T

K

V

L

N

R

G

S

-

R

D

R

P

P

H

N

C

C

P

A

V

V

C

C

A

S

G82 (= G22) mutation to D: In cnxF21ts and cnxF24ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.
G100 (= G40) mutation to S: In cnxF1285; impairs molybdopterin biosynthesis.
R130 (= R70) mutation to Q: In cnxF200; impairs molybdopterin biosynthesis.
C185 (≠ A125) mutation to Y: In cnxF472; impairs molybdopterin biosynthesis.
E215 (= E155) mutation to K: In cnxF119; impairs molybdopterin biosynthesis.
G264 (= G194) mutation to S: In cnxF142ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.

6yubB Crystal structure of uba4 from chaetomium thermophilum (see paper)
38% identity, 84% coverage: 1:208/247 of query aligns to 7:212/289 of 6yubB

query
sites
6yubB

M

L

N

S

D

K

E

E

Q

E

L

I

M

E

R

R

Y

Y

S

S

R

R

Q

Q

I

M

M

I

L

V

P

P

E

G

L

M

D

G

I

K

T

E

G

G

Q

Q

E

L

R

R

L

L

A

M

A

N

S

A

R

K

A

V

L

L

I

I

V

I

G

G

A

A

G

G

L

L

G

G

S

C

P

P

V

A

A

A

L

Q

Y

Y

L

L

G

A

A

G

A

A

G

G

V

V

G

G

E

T

L

I

R

G

I

I

A

V

D

D

D

G

D

D

V

S

V

V

D

E

L

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

I

V

A

A

H

H

R

A

Q

T

D

K

A

R

L

V

G

G

Q

M

P

L

K

K

A

V

A

D

S

S

A

L

T

I

R

T

A

H

V

L

T

I

A

E

R

I

N

N

P

P

E

L

I

P

T

V

V

Y

T

V

P

P

L

Y

E

R

E

F

R

D

L

L

D

T

G

P

E

Q

R

N

L

A

G

A

A

Q

E

I

V

I

G

K

A

P

A

W

D

D

V

V

V

I

I

L

D

D

A

C

T

T

D

D

N

N

F

P

A

A

T

T

R

R

F

Y

A

L

L

I

N

S

A

D

A

V

C

C

V

V

A

L

H

G

R

K

P

P

L

L

V

V

S

S

A

A

V

S

I

V

R

Q

W

K

E

S

L

G

Q

Q

V

L

S

I

A

V

F

L

R

N

-

C

-

P

-

T

P

P

G

Q

G

G

-

V

-

N

-

K

-

A

R

A

P

P

C
|
C

Y

Y

R

R

C
|
C

I

C

Y

F

A

-

E

-

G

-

D

-

E

-

P

-

Q

-

L

-

T

-

C

-

S

-

E

-

S

K

G

G

V

I

L

M

G

G

P

P

L

V

P

V

G

G

V

M

A

M

G

G

S

V

L

A

E

Q

A

A

V

G

E

E

A

A

I

I

K

K

I

I

L

L

binding zinc ion: C184 (= C168), C187 (= C171)

Sites not aligning to the query:

binding zinc ion: 265

6yubA Crystal structure of uba4 from chaetomium thermophilum (see paper)
38% identity, 84% coverage: 1:208/247 of query aligns to 6:213/423 of 6yubA

query
sites
6yubA

M

L

N

S

D

K

E

E

Q

E

L

I

M

E

R

R

Y

Y

S

S

R

R

Q

Q

I

M

M

I

L

V

P

P

E

G

L

M

D

G

I

K

T

E

G

G

Q

Q

E

L

R

R

L

L

A

M

A

N

S

A

R

K

A

V

L

L

I

I

V

I

G

G

A

A

G

G

L

L

G

G

S

C

P

P

V

A

A

A

L

Q

Y

Y

L

L

G

A

A

G

A

A

G

G

V

V

G

G

E

T

L

I

R

G

I

I

A

V

D

D

D

G

D

D

V

S

V

V

D

E

L

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

I

V

A

A

H

H

R

A

Q

T

D

K

A

R

L

V

G

G

Q

M

P

L

K

K

A

V

A

D

S

S

A

L

T

I

R

T

A

H

V

L

T

I

A

E

R

I

N

N

P

P

E

L

I

P

T

V

V

Y

T

V

P

P

L

Y

E

R

E

F

R

D

L

L

D

T

G

P

E

Q

R

N

L

A

G

A

A

Q

E

I

V

I

G

K

A

P

A

W

D

D

V

V

V

I

I

L

D

D

A

C

T

T

D

D

N

N

F

P

A

A

T

T

R

R

F

Y

A

L

L

I

N

S

A

D

A

V

C

C

V

V

A

L

H

G

R

K

P

P

L

L

V

V

S

S

A

A

V

S

I

V

R

Q

W

K

E

S

L

G

Q

Q

V

L

S

I

A

V

F

L

R

N

-

C

-

P

-

T

P

P

G

Q

G

G

-

V

-

N

-

K

-

A

R

A

P

P

C
|
C

Y

Y

R

R

C
|
C

I

C

Y

F

A

-

E

-

G

-

D

-

E

-

P

-

Q

-

L

-

T

-

C

-

S

K

E

K

S

P

G

G

V

I

L

M

G

G

P

P

L

V

P

V

G

G

V

M

A

M

G

G

S

V

L

A

E

Q

A

A

V

G

E

E

A

A

I

I

K

K

I

I

L

L

binding zinc ion: C183 (= C168), C186 (= C171)

Sites not aligning to the query:

binding zinc ion: 266, 269

P38820 Adenylyltransferase and sulfurtransferase UBA4; Needs CLA4 to survive protein 3; Ubiquitin-like protein activator 4; EC 2.7.7.-; EC 2.8.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
37% identity, 85% coverage: 4:213/247 of query aligns to 42:255/440 of P38820

query
sites
P38820

E

E

Q

E

L

Y

M

Q

R

R

Y

Y

S

G

R

R

Q

Q

I

M

M

I

L

V

P

E

E

E

L

T

D

G

-

G

I

V

T

A

G

G

Q

Q

E

V

R

K

L

L

A

K

A

N

S

T

R

K

A

V

L

L

I

V

V

V

G

G

A

A

G

G

L

L

G

G

S

C

P

P

V

A

A

L

L

P

Y

Y

L

L

G

A

A

G

A

A

G

G

V

V

G

G

E

Q

L

I

R

G

I

I

A

V

D

D

D

N

D

D

V

V

D

E

L

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

I

V

A

L

H

H

R

D

Q

S

D

S

A

R

L

V

G

G

Q

M

P

L

K

K

A

C

A

E

S

S

A

A

T

R

R

Q

A

Y

V

I

T

T

A

K

R

L

N

N

P

P

E

H

I

I

T

N

V

V

T

V

P

T

L

Y

E

P

E

V

R

R

L

L

D

N

G

S

E

S

R

N

L

A

G

F

A

D

E

I

V

F

G

K

A

G

A

Y

D

N

V

Y

V

I

I

L

D

D

A

C

T

T

D

D

N

S

F

P

A

L

T

T

R

R

F

Y

A

L

L

V

N

S

A

D

A

V

C

A

V

V

A

N

A

L

H

G

R

I

P

T

L

V

V

V

S

S

A

A

A

S

V

G

I

L

R

G

W

T

E

E

L

G

Q

Q

V

L

S

T

-

I

-

L

A

N

F

F

R

N

P

N

G

I

G

G

R

-

P

P

C

C

Y

Y

R

R

C

C

I

F

Y

Y

A

P

E

T

G

P

D

P

E

P

P

P

Q

N

-

A

-

V

L

T

T

S

C
|
C

S

Q

E

E

S

G

G

G

V

V

L

I

G

G

P

P

L

C

P

I

G

G

V

L

A

V

G

G

S

T

L

M

E

M

A

A

V

V

E

E

A

T

I

L

K

K

I

L

L

I

T

L

G

G

A

I

G

Y

T

T

C225 (= C183) mutation C->A,S: Abolishes adenylyltransferase activity but not sulfurtransferase activity.

Sites not aligning to the query:

397 mutation C->A,S: Abolishes sulfurtransferase activity but not adenylyltransferase activity.

3h9jA Crystal structure of e. Coli mccb + ampcpp + semet mcca (see paper)
25% identity, 98% coverage: 2:243/247 of query aligns to 85:337/339 of 3h9jA

query
sites
3h9jA

N

N

D

S

E

T

Q

E

L

N

M

N

R

R

Y

Y

S

S

R

R

Q

N

I

F

M

L

L

H

P

Y

E

Q

L

S

D

Y

-

G

-

A

-

N

-

P

I

V

T

L

G

V

Q

Q

E

D

R

K

L

L

A

K

A

N

S

A

R

K

A

V

L

V

I

I

V

L

G

G

A

C

G
|
G

L

I

G

G

S

N

P

H

V

V

A

S

L

V

Y

I

L

L

G

A

A

T

A

S

G

G

V

I

G

G

E

E

L

I

R

I

I

L

A

I

D
|
D

D

N

D
|
D

V

Q

V

I

D

E

L

N

S

T

N

N

L

L

Q

T

R
|
R

Q

Q

I

V

A

L

H

F

R

S

Q

E

D

D

A

D

L

V

G

G

Q

K

P

N

K
|
K

A

T

A

E

S

V

A

I

T

K

R

R

A

E

V

L

T

L

A

K

R

R

N

N

P

S

E

E

I

I

T

S

V

V

T

S

P

E

L

I

E

A

E

L

R
x
N

L
x
I

D

N

G

D

E

Y

R

T

L

D

G

L

A

H

E

K

V

V

G

P

A

E

A

A

D

D

V

I

V

W

I

V

D

V

A

S

T
x
A

D
|
D

N
x
H

-

P

F

F

A

N

T

L

R

I

F

N

A

W

L

V

N

N

A

K

A

Y

C

C

V

V

A

R

A

A

H

N

R

Q

P

P

L

Y

V

I

S

N

A

A

A

G

V

Y

I

V

R

N

W

-

E

D

L

I

Q

A

V

V

S

F

A

G

-

P

-

L

F

Y

R

V

P

P

G

G

G

K

R

T

P

G

C
|
C

Y

Y

R

E

C

C

I

Q

Y

K

A

K

E

E

G

N

D

I

E

D

P

H

Q

K

L

I

T

K

C

L

S

I

E

N

S

S

-

R

-

F

-

K

-

P

G

A

V

T

L

F

G

A

P

P

L

V

P

N

G

N

V

V

A

A

G

A

S

A

L

L

E

C

A

A

V

A

E

D

A

V

I

I

K

K

I

F

L

I

T

G

G

K

A

Y

G

S

T

E

P

P

L

L

-

S

F

L

G

N

R

K

L
x
R

L

I

V
x
G

V

I

D

W

A

S

L

D

R

E

M

I

R

K

L

I

R

H

T

S

L

Q

T

N

V

M

N

G

G

R

D

S

P

P

H

V

C
|
C

P

S

V

V

C
|
C

active site: R157 (= R70)
binding diphosphomethylphosphonic acid adenosyl ester: G124 (= G37), G125 (= G38), D146 (= D59), D148 (= D61), R157 (= R70), K170 (= K83), N193 (≠ R106), I194 (≠ L107), A213 (≠ T126), D214 (= D127), H215 (≠ N128)
binding zinc ion: C257 (= C168), C334 (= C240), C337 (= C243)
binding : R313 (≠ L219), G315 (≠ V221)

3h5nA Crystal structure of e. Coli mccb + atp (see paper)
25% identity, 98% coverage: 2:243/247 of query aligns to 85:337/338 of 3h5nA

query
sites
3h5nA

N

N

D

S

E

T

Q

E

L

N

M

N

R

R

Y

Y

S

S

R

R

Q

N

I

F

M

L

L

H

P

Y

E

Q

L

S

D

Y

-

G

-

A

-

N

-

P

I

V

T

L

G

V

Q

Q

E

D

R

K

L

L

A

K

A

N

S

A

R

K

A

V

L

V

I

I

V

L

G

G

A

C

G
|
G

G

G

L

I

G

G

S

N

P

H

V

V

A

S

L

V

Y

I

L

L

G

A

A

T

A

S

G

G

V

I

G

G

E

E

L

I

R

I

I

L

A

I

D
|
D

D

N

D

D

V

Q

V

I

D

E

L

N

S

T

N
|
N

L

L

Q

T

R
|
R

Q
|
Q

I

V

A

L

H

F

R

S

Q

E

D

D

A

D

L

V

G

G

Q

K

P

N

K
|
K

A

T

A

E

S

V

A

I

T

K

R

R

A

E

V

L

T

L

A

K

R

R

N

N

P

S

E

E

I

I

T

S

V

V

T

S

P

E

L

I

E

A

E

L

R
x
N

L
x
I

D

N

G

D

E

Y

R

T

L

D

G

L

A

H

E

K

V

V

G

P

A

E

A

A

D

D

V

I

V

W

I

V

D

V

A

S

T
x
A

D
|
D

N
x
H

-

P

F

F

A

N

T

L

R

I

F

N

A

W

L

V

N

N

A

K

A

Y

C

C

V

V

A

R

A

A

H

N

R

Q

P

P

L

Y

V

I

S

N

A

A

A

G

V

Y

I

V

R

N

W

-

E

D

L

I

Q

A

V

V

S

F

A

G

-

P

-

L

F

Y

R

V

P

P

G

G

G

K

R

T

P

G

C
|
C

Y

Y

R

E

C
|
C

I

Q

Y

K

A

K

E

E

G

N

D

I

E

D

P

H

Q

K

L

I

T

K

C

L

S

I

E

N

S

S

-

R

-

F

-

K

-

P

G

A

V

T

L

F

G

A

P

P

L

V

P

N

G

N

V

V

A

A

G

A

S

A

L

L

E

C

A

A

V

A

E

D

A

V

I

I

K

K

I

F

L

I

T

G

G

K

A

Y

G

S

T

E

P

P

L

L

-

S

F

L

G

N

R

K

L

R

L

I

V

G

V

I

D

W

A

S

L

D

R

E

M

I

R

K

L

I

R

H

T

S

L

Q

T

N

V

M

N

G

G

R

D

S

P

P

H

V

C
|
C

P

S

V

V

C
|
C

active site: R157 (= R70)
binding adenosine-5'-triphosphate: G124 (= G37), D146 (= D59), N154 (= N67), R157 (= R70), Q158 (= Q71), K170 (= K83), N193 (≠ R106), I194 (≠ L107), A213 (≠ T126), D214 (= D127), H215 (≠ N128)
binding zinc ion: C257 (= C168), C260 (= C171), C334 (= C240), C337 (= C243)

3h5rA Crystal structure of e. Coli mccb + succinimide (see paper)
25% identity, 98% coverage: 2:243/247 of query aligns to 85:338/340 of 3h5rA

query
sites
3h5rA

N

N

D

S

E

T

Q

E

L

N

M

N

R

R

Y

Y

S

S

R

R

Q

N

I

F

M

L

L

H

P

Y

E

Q

L

S

D

Y

-

G

-

A

-

N

-

P

I

V

T

L

G

V

Q

Q

E

D

R

K

L

L

A

K

A

N

S

A

R

K

A

V

L

V

I

I

V

L

G

G

A

C

G

G

L
x
I

G

G

S

N

P

H

V

V

A

S

L

V

Y

I

L

L

G

A

A

T

A

S

G

G

V

I

G

G

E

E

L

I

R

I

I

L

A

I

D

D

D

N

D

D

V

Q

V

I

D

E

L

N

S

T

N

N

L

L

Q

T

R
|
R

Q

Q

I

V

A

L

H

F

R

S

Q

E

D

D

A

D

L

V

G

G

Q

K

P

N

K

K

A

T

A

E

S

V

A

I

T

K

R

R

A

E

V

L

T

L

A

K

R

R

N

N

P

S

E

E

I

I

T

S

V

V

T

S

P

E

L

I

E

A

E

L

R

N

L

I

D

N

G

D

E

Y

R

T

L

D

G

L

A

H

E

K

V

V

G

P

A

E

A

A

D

D

V

I

V

W

I

V

D

V

A
x
S

T
x
A

D

D

N

H

-

P

F

F

A

N

T

L

R

I

F

N

A

W

L

V

N

N

A

K

A

Y

C

C

V

V

A

R

A

A

H

N

R

Q

P

P

L

Y

V

I

S

N

A
|
A

A
x
G

V
x
Y

I

V

R

N

W

-

E

D

L

I

Q

A

V
|
V

S

F

A

G

-

P

-

L

F

Y

R

V

P

P

G

G

G

K

R

T

P

G

C
|
C

Y

Y

R

E

C
|
C

-

Q

I

K

Y

V

A

K

E

E

G

N

D

I

E

D

P

H

Q

K

L

I

T

K

C

L

S

I

E

N

S

S

-

R

-

F

-

K

-
x
P

G

A

V

T

L

F

G

A

P

P

L

V

P

N

G

N

V

V

A

A

G

A

S

A

L

L

E

C

A

A

V

A

E

D

A

V

I

I

K

K

I

F

L

I

T

G

G

K

A

Y

G

S

T

E

P

P

L

L

-

S

F

L

G

N

R

K

L
x
R

L

I

V
x
G

V

I

D

W

A

S

L

D

R

E

M

I

R

K

L

I

R

H

T

S

L

Q

T

N

V

M

N

G

G

R

D

S

P

P

H

V

C
|
C

P

S

V

V

C
|
C

active site: R157 (= R70)
binding zinc ion: C257 (= C168), C260 (= C171), C335 (= C240), C338 (= C243)
binding : I126 (≠ L39), S212 (≠ A125), A213 (≠ T126), A237 (= A149), G238 (≠ A150), Y239 (≠ V151), V245 (= V158), P280 (vs. gap), R314 (≠ L219), G316 (≠ V221)

Sites not aligning to the query:

binding : 10, 14, 26

3h5aC Crystal structure of e. Coli mccb (see paper)
25% identity, 98% coverage: 2:243/247 of query aligns to 85:346/358 of 3h5aC

query
sites
3h5aC

N

N

D

S

E

T

Q

E

L

N

M

N

R

R

Y

Y

S

S

R

R

Q

N

I

F

M

L

L

H

P

Y

E

Q

L

S

D

Y

-

G

-

A

-

N

-

P

I

V

T

L

G

V

Q

Q

E

D

R

K

L

L

A

K

A

N

S

A

R

K

A

V

L

V

I

I

V

L

G

G

A

C

G

G

L

I

G

G

S

N

P

H

V

V

A

S

L

V

Y

I

L

L

G

A

A

T

A

S

G

G

V

I

G

G

E

E

L

I

R

I

I

L

A

I

D

D

D

N

D

D

V

Q

V

I

D

E

L

N

S

T

N

N

L

L

Q

T

R
|
R

Q

Q

I

V

A

L

H

F

R

S

Q

E

D

D

A

D

L

V

G

G

Q

K

P

N

K

K

A

T

A

E

S

V

A

I

T

K

R

R

A

E

V

L

T

L

A

K

R

R

N

N

P

S

E

E

I

I

T

S

V

V

T

S

P

E

L

I

E

A

E

L

R

N

L

I

D

N

G

D

E

Y

R

T

L

D

G

L

A

H

E

K

V

V

G

P

A

E

A

A

D

D

V

I

V

W

I

V

D

V

A

S

T

A

D

D

N

H

-

P

F

F

A

N

T

L

R

I

F

N

A

W

L

V

N

N

A

K

A

Y

C

C

V

V

A

R

A

A

H

N

R

Q

P

P

L

Y

V

I

S

N

A

A

A

G

V

Y

I

V

R

N

W

-

E

D

L

I

Q

A

V

V

S

F

A

G

-

P

-

L

F

Y

R

V

P

P

G

G

G

K

R

T

P

G

C
|
C

Y

Y

R

E

C
|
C

-

Q

-

K

-

V

-

A

-

D

I

L

Y

Y

A

G

E

S

G

E

D

K

E

E

P

N

-

I

-

D

-

H

-

K

-

I

Q

K

L

L

T

I

C

N

S

S

-

R

-

F

E

K

S

P

G

A

V

T

L

F

G

A

P

P

L

V

P

N

G

N

V

V

A

A

G

A

S

A

L

L

E

C

A

A

V

A

E

D

A

V

I

I

K

K

I

F

L

I

T

G

G

K

A

Y

G

S

T

E

P

P

L

L

-

S

F

L

G

N

R

K

L

R

L

I

V

G

V

I

D

W

A

S

L

D

R

E

M

I

R

K

L

I

R

H

T

S

L

Q

T

N

V

M

N

G

G

R

D

S

P

P

H

V

C
|
C

P

S

V

V

C

C

active site: R157 (= R70)
binding zinc ion: C257 (= C168), C260 (= C171), C343 (= C240)

6om4B The structure of microcin c7 biosynthetic enzyme mccb in complex with n-formylated mcca (see paper)
25% identity, 89% coverage: 23:243/247 of query aligns to 107:343/344 of 6om4B

query
sites
6om4B

Q

Q

E

D

R

K

L

L

A

K

A

N

S

A

R

K

A

V

L

V

I

I

V

L

G

G

A

C

G
|
G

G

G

L
x
I

G

G

S

N

P

H

V

V

A

S

L

V

Y

I

L

L

G

A

A

T

A

S

G

G

V

I

G

G

E

E

L

I

R

I

I

L

A

I

D
|
D

D
x
N

D
|
D

V

Q

V

I

D
x
E

L

N

S

T

N
|
N

L

L

Q

T

R
|
R

Q
|
Q

I

V

A

L

H

F

R

S

Q

E

D

D

A

D

L

V

G

G

Q

K

P

N

K
|
K

A

T

A

E

S

V

A

I

T

K

R

R

A

E

V

L

T

L

A

K

R

R

N

N

P

S

E

E

I

I

T

S

V

V

T

S

P

E

L

I

E

A

E

L

R

N

L
x
I

D

N

G

D

E

Y

R

T

L

D

G

L

A

H

E

K

V

V

G

P

A

E

A

A

D

D

V

I

V

W

I

V

D

V

A
x
S

T
x
A

D
|
D

N
x
H

-

P

F

F

A

N

T

L

R

I

F

N

A

W

L

V

N

N

A

K

A

Y

C

C

V

V

A

R

A

A

H

N

R

Q

P

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C

binding magnesium ion: D145 (= D61), E148 (≠ D64)
binding 5'-O-[(S)-amino(hydroxy)phosphoryl]adenosine: G121 (= G37), D143 (= D59), N144 (≠ D60), K167 (= K83), I191 (≠ L107), S209 (≠ A125), A210 (≠ T126), D211 (= D127), H212 (≠ N128)
binding pyrophosphate 2-: N151 (= N67), R154 (= R70), Q155 (= Q71), K167 (= K83)
binding zinc ion: C254 (= C168), C257 (= C171), C340 (= C240), C343 (= C243)
binding : I123 (≠ L39), S209 (≠ A125), A210 (≠ T126), D211 (= D127), N233 (≠ S148), A234 (= A149), G235 (≠ A150), Y236 (≠ V151), V237 (≠ I152), V242 (= V158), V261 (vs. gap), A262 (vs. gap), Y265 (= Y173), R319 (≠ L219), G321 (≠ V221), W323 (≠ D223), Q332 (≠ L232)

Sites not aligning to the query:

binding : 10, 14, 23, 26

Query Sequence

>WP_011813799.1 NCBI__GCF_000015585.1:WP_011813799.1
MNDEQLMRYSRQIMLPELDITGQERLAASRALIVGAGGLGSPVALYLGAAGVGELRIADD
DVVDLSNLQRQIAHRQDALGQPKAASATRAVTARNPEITVTPLEERLDGERLGAEVGAAD
VVIDATDNFATRFALNAACVAAHRPLVSAAVIRWELQVSAFRPGGRPCYRCIYAEGDEPQ
LTCSESGVLGPLPGVAGSLEAVEAIKILTGAGTPLFGRLLVVDALRMRLRTLTVNGDPHC
PVCAEAP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory