SitesBLAST
Comparing WP_011814191.1 NCBI__GCF_000015585.1:WP_011814191.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7cmyC Isocitrate lyase from bacillus cereus atcc 14579 in complex with magnessium ion, glyoxylate, and succinate
72% identity, 98% coverage: 8:422/422 of query aligns to 8:417/417 of 7cmyC
6lrtA Crystal structure of isocitrate lyase (caur_3889) from chloroflexus aurantiacus in complex with isocitrate and manganese ion
72% identity, 98% coverage: 8:422/422 of query aligns to 10:423/423 of 6lrtA
P0A9G6 Isocitrate lyase; ICL; Isocitrase; Isocitratase; EC 4.1.3.1 from Escherichia coli (strain K12) (see 3 papers)
69% identity, 98% coverage: 8:422/422 of query aligns to 11:434/434 of P0A9G6
- SGW 91:93 (= SGW 86:88) binding substrate
- D157 (= D147) binding Mg(2+)
- C195 (= C185) active site, Proton acceptor; mutation to A: Large decrease in activity.; mutation to S: Large decrease in activity.
- A219 (= A209) mutation to C: Isocitrate lyase activity is reduced compared to the wild-type.
- R232 (= R222) binding substrate
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
6xppA Crystal structure of itaconate modified mycobaterium tuberculosis isocitrate lyase (see paper)
66% identity, 98% coverage: 8:422/422 of query aligns to 12:426/426 of 6xppA
- active site: Y88 (= Y84), D107 (= D103), D152 (= D147), E154 (= E149), H179 (= H174), E181 (= E176), C190 (= C185), H192 (= H187), R227 (= R222), E284 (= E279), Q307 (≠ K302), S314 (= S309), S316 (= S311)
- binding 2-methylidenebutanedioic acid: W92 (= W88), C190 (= C185), H192 (= H187), R227 (= R222), N312 (= N307), S314 (= S309), S316 (= S311), T346 (= T341)
- binding magnesium ion: A275 (= A270), A278 (= A273), Q307 (≠ K302)
7rb1A Isocitrate lyase-1 from mycobacterium tuberculosis covalently modified by 5-descarboxy-5-nitro-d-isocitric acid (see paper)
66% identity, 98% coverage: 8:422/422 of query aligns to 13:427/427 of 7rb1A
- binding dihydroxyacetic acid: Y89 (= Y84), S91 (= S86), W93 (= W88), D153 (= D147), R228 (= R222), T347 (= T341)
- binding (3E)-3-(hydroxyimino)propanoic acid: C191 (= C185), G192 (= G186), H193 (= H187), R228 (= R222), S315 (= S309), S317 (= S311), T347 (= T341)
- binding magnesium ion: A276 (= A270), A279 (= A273), Q308 (≠ K302)
6wsiA Intact cis-2,3-epoxysuccinic acid bound to isocitrate lyase-1 from mycobacterium tuberculosis (see paper)
66% identity, 98% coverage: 8:422/422 of query aligns to 13:427/427 of 6wsiA
- active site: Y89 (= Y84), D108 (= D103), D153 (= D147), E155 (= E149), H180 (= H174), E182 (= E176), C191 (= C185), H193 (= H187), R228 (= R222), E285 (= E279), Q308 (≠ K302), S315 (= S309), S317 (= S311)
- binding magnesium ion: A276 (= A270), A279 (= A273), Q308 (≠ K302)
- binding (2R,3S)-oxirane-2,3-dicarboxylic acid: C191 (= C185), G192 (= G186), H193 (= H187), R228 (= R222), E285 (= E279), N313 (= N307), S315 (= S309), S317 (= S311), T347 (= T341)
6vb9A Covalent adduct of cis-2,3-epoxysuccinic acid with isocitrate lyase-1 from mycobacterium tuberculosis (see paper)
66% identity, 98% coverage: 8:422/422 of query aligns to 13:427/427 of 6vb9A
- active site: Y89 (= Y84), D108 (= D103), D153 (= D147), E155 (= E149), H180 (= H174), E182 (= E176), C191 (= C185), H193 (= H187), R228 (= R222), E285 (= E279), Q308 (≠ K302), S315 (= S309), S317 (= S311)
- binding magnesium ion: A276 (= A270), A279 (= A273), Q308 (≠ K302)
- binding oxalic acid: Y89 (= Y84), S91 (= S86), G92 (= G87), W93 (= W88), D153 (= D147), C191 (= C185), R228 (= R222), W283 (= W277), T347 (= T341)
5dqlA Crystal structure of 2-vinyl glyoxylate modified isocitrate lyase from mycobacterium tuberculosis (see paper)
66% identity, 98% coverage: 8:422/422 of query aligns to 13:427/427 of 5dqlA
- active site: Y89 (= Y84), D108 (= D103), D153 (= D147), E155 (= E149), H180 (= H174), E182 (= E176), C191 (= C185), H193 (= H187), R228 (= R222), E285 (= E279), Q308 (≠ K302), S315 (= S309), S317 (= S311)
- binding magnesium ion: A276 (= A270), A279 (= A273), Q308 (≠ K302)
- binding 4-hydroxy-2-oxobutanoic acid: W93 (= W88), D108 (= D103), C191 (= C185), H193 (= H187), S315 (= S309), S317 (= S311), T347 (= T341), L348 (= L342)
6c4aA Crystal structure of 3-nitropropionate modified isocitrate lyase from mycobacterium tuberculosis with pyruvate (see paper)
66% identity, 98% coverage: 8:422/422 of query aligns to 14:428/428 of 6c4aA
- active site: Y90 (= Y84), D109 (= D103), D154 (= D147), E156 (= E149), H181 (= H174), E183 (= E176), C192 (= C185), H194 (= H187), R229 (= R222), E286 (= E279), Q309 (≠ K302), S316 (= S309), S318 (= S311)
- binding 3-nitropropanoic acid: Y357 (= Y350), S358 (= S351), R380 (≠ A373)
- binding magnesium ion: A277 (= A270), A280 (= A273), Q309 (≠ K302)
- binding pyruvic acid: Y90 (= Y84), S92 (= S86), G93 (= G87), W94 (= W88), D154 (= D147), C192 (= C185), R229 (= R222), W284 (= W277), T348 (= T341)
P9WKK7 Isocitrate lyase; ICL; Isocitrase; Isocitratase; EC 4.1.3.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
66% identity, 98% coverage: 8:422/422 of query aligns to 13:427/428 of P9WKK7
- SGW 91:93 (= SGW 86:88) binding substrate
- D153 (= D147) binding Mg(2+)
- C191 (= C185) mutation to S: Adopts a conformation almost identical to the wild-type.
- GH 192:193 (= GH 186:187) binding substrate
- R228 (= R222) binding substrate
- NCSPS 313:317 (= NCSPS 307:311) binding substrate
- K334 (≠ D328) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
- T347 (= T341) binding substrate
1f8iA Crystal structure of isocitrate lyase:nitropropionate:glyoxylate complex from mycobacterium tuberculosis (see paper)
66% identity, 98% coverage: 8:422/422 of query aligns to 13:427/427 of 1f8iA
- active site: Y89 (= Y84), D108 (= D103), D153 (= D147), E155 (= E149), H180 (= H174), E182 (= E176), S191 (≠ C185), H193 (= H187), R228 (= R222), E285 (= E279), Q308 (≠ K302), S315 (= S309), S317 (= S311)
- binding glyoxylic acid: Y89 (= Y84), S91 (= S86), W93 (= W88), D153 (= D147), T347 (= T341)
1igwC Crystal structure of the isocitrate lyase from the a219c mutant of escherichia coli (see paper)
68% identity, 94% coverage: 8:405/422 of query aligns to 10:416/416 of 1igwC
- active site: Y88 (= Y84), D107 (= D103), D156 (= D147), E158 (= E149), H183 (= H174), E185 (= E176), C194 (= C185), R231 (= R222), E288 (= E279), K311 (= K302), S318 (= S309), S320 (= S311)
- binding pyruvic acid: S90 (= S86), G91 (= G87), W92 (= W88), D156 (= D147), R231 (= R222), T350 (= T341)
7rbxC Crystal structure of isocitrate lyase and phosphorylmutase:isocitrate lyase from brucella melitensis biovar abortus 2308 bound to itaconic acid (see paper)
68% identity, 96% coverage: 17:422/422 of query aligns to 14:423/425 of 7rbxC
1igwA Crystal structure of the isocitrate lyase from the a219c mutant of escherichia coli (see paper)
64% identity, 94% coverage: 8:405/422 of query aligns to 10:396/396 of 1igwA
- active site: Y88 (= Y84), D107 (= D103), D156 (= D147), E158 (= E149), H183 (= H174), E185 (= E176), C194 (= C185), R227 (= R222), E284 (= E279), K307 (= K302)
- binding pyruvic acid: S90 (= S86), W92 (= W88), D156 (= D147), R227 (= R222), T330 (= T341)
5e9gD Structural insights of isocitrate lyases from magnaporthe oryzae (see paper)
35% identity, 94% coverage: 10:405/422 of query aligns to 24:486/486 of 5e9gD
- active site: Y100 (= Y84), D119 (= D103), D173 (= D147), D175 (≠ E149), H200 (= H174), E202 (= E176), C211 (= C185), H213 (= H187), R248 (= R222), E363 (= E279), Q386 (≠ K302), S393 (= S309), S395 (= S311)
- binding glyoxylic acid: Y100 (= Y84), S102 (= S86), G103 (= G87), W104 (= W88), D173 (= D147), H200 (= H174), R248 (= R222), T424 (= T341)
- binding glycerol: C211 (= C185), G212 (= G186), H213 (= H187), R248 (= R222)
5e9fD Structural insights of isocitrate lyases from magnaporthe oryzae (see paper)
35% identity, 93% coverage: 10:403/422 of query aligns to 23:452/453 of 5e9fD
- active site: Y99 (= Y84), D118 (= D103), D172 (= D147), D174 (≠ E149), H199 (= H174), E201 (= E176), R240 (= R222), E330 (= E279), Q353 (≠ K302), S360 (= S309), S362 (= S311)
- binding magnesium ion: D118 (= D103), D172 (= D147)
6edwB Crystal structure of mycobacterium tuberculosis icl2 in the apo form (see paper)
38% identity, 58% coverage: 3:245/422 of query aligns to 9:264/746 of 6edwB
Sites not aligning to the query:
6edzA Crystal structure of mycobacterium tuberculosis icl2 in complex with acetyl-coa, form i (see paper)
38% identity, 58% coverage: 3:245/422 of query aligns to 9:264/733 of 6edzA
Sites not aligning to the query:
- active site: 437, 460, 467, 469
- binding acetyl coenzyme *a: 643, 644, 645, 646, 652, 653, 655, 657, 658, 679, 681, 682, 684, 688, 692, 732
7ebeA Crystal structure of isocitrate lyase-1 from candida albicans (see paper)
38% identity, 59% coverage: 3:251/422 of query aligns to 17:276/544 of 7ebeA
- active site: Y99 (= Y84), D118 (= D103), D172 (= D147), D174 (≠ E149), H199 (= H174), E201 (= E176), C210 (= C185), H212 (= H187), R247 (= R222)
- binding magnesium ion: G102 (= G87), W103 (= W88), D172 (= D147)
Sites not aligning to the query:
6edwC Crystal structure of mycobacterium tuberculosis icl2 in the apo form (see paper)
38% identity, 58% coverage: 3:245/422 of query aligns to 9:264/724 of 6edwC
Sites not aligning to the query:
Query Sequence
>WP_011814191.1 NCBI__GCF_000015585.1:WP_011814191.1
MLKDQAAIERDWAENPRWKGVQRGYGADEVVRLRGTVHVEYSLARQGAEKLWQSMHEMPY
VNALGALTGNQALQQVKAGLNAIYLSGWQVAADANLGQTMYPDQSLYPANSVPAVVDRIN
NALLRADEINHAEGNPPFDFMKPIVADAEAGFGGVLNAFELMKGMIRAGAAGVHFEDQLA
SVKKCGHMGGKVLLPTQEAVQKLIAARLAADTMDVPTILVARTDAEAADLLTSDVDDNDK
PFITGERTAEGFFRTKPGIEQAISRGLAYAPYADVIWCETGKPDLEFAREFAQAIHEKYP
GKLLAYNCSPSFNWAGNLDEATIRKFQDELGKMGFKFQFITLAGFHSLNYSMFELARGYK
ERQMEAYSELQQAEFAAEKHGYTATRHQREVGAGYFDQVTNVIQGGQSSVTALKGSTEEE
QF
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory