SitesBLAST
Comparing WP_011814258.1 NCBI__GCF_000015585.1:WP_011814258.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3jtxB Crystal structure of aminotransferase (np_283882.1) from neisseria meningitidis z2491 at 1.91 a resolution
48% identity, 98% coverage: 1:390/399 of query aligns to 1:388/393 of 3jtxB
2x5dD Crystal structure of a probable aminotransferase from pseudomonas aeruginosa (see paper)
27% identity, 95% coverage: 17:394/399 of query aligns to 7:368/380 of 2x5dD
- active site: Y113 (= Y126), D191 (= D203), A193 (≠ C205), K225 (= K243)
- binding pyridoxal-5'-phosphate: G87 (= G99), S88 (≠ T100), K89 (≠ R101), Y113 (= Y126), D191 (= D203), A193 (≠ C205), Y194 (= Y206), T222 (≠ S240), S224 (= S242), K225 (= K243), R233 (= R251)
2o1bA Structure of aminotransferase from staphylococcus aureus
26% identity, 93% coverage: 26:395/399 of query aligns to 17:373/376 of 2o1bA
- active site: Y115 (= Y126), D192 (= D203), A194 (≠ C205), K225 (= K243)
- binding pyridoxal-5'-phosphate: T90 (= T100), K91 (≠ R101), Y115 (= Y126), N164 (= N175), D192 (= D203), A194 (≠ C205), Y195 (= Y206), S222 (= S240), S224 (= S242), K225 (= K243), R233 (= R251)
1gdeA Crystal structure of pyrococcus protein a-1 e-form (see paper)
26% identity, 93% coverage: 25:395/399 of query aligns to 22:383/388 of 1gdeA
- active site: K232 (= K243)
- binding glutamic acid: F120 (≠ Y126), N170 (= N175), R361 (= R373)
- binding pyridoxal-5'-phosphate: G94 (= G99), A95 (≠ T100), N96 (≠ R101), F120 (≠ Y126), N166 (= N171), D198 (= D203), Y201 (= Y206), S231 (= S242), K232 (= K243), R240 (= R251)
1gd9A Crystall structure of pyrococcus protein-a1 (see paper)
26% identity, 93% coverage: 25:395/399 of query aligns to 22:383/388 of 1gd9A
- active site: K232 (= K243)
- binding pyridoxal-5'-phosphate: G94 (= G99), A95 (≠ T100), N96 (≠ R101), F120 (≠ Y126), N170 (= N175), D198 (= D203), V200 (≠ C205), Y201 (= Y206), S231 (= S242), K232 (= K243), R240 (= R251)
6l1nA Substrate bound bacf structure from bacillus subtillis (see paper)
28% identity, 95% coverage: 13:392/399 of query aligns to 21:385/393 of 6l1nA
- binding glycine: Y127 (= Y126), N177 (= N175), Y324 (= Y327), R366 (= R373)
- binding pyridoxal-5'-phosphate: G102 (≠ T100), Y127 (= Y126), N173 (= N171), N177 (= N175), D205 (= D203), Y208 (= Y206), S235 (= S240), S237 (= S242), R246 (= R251)
Sites not aligning to the query:
6l1oB Product bound bacf structure from bacillus subtillis (see paper)
28% identity, 91% coverage: 28:392/399 of query aligns to 32:386/392 of 6l1oB
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G103 (≠ T100), K104 (≠ R101), Y128 (= Y126), N174 (= N171), D206 (= D203), Y209 (= Y206), S236 (= S240), S238 (= S242), K239 (= K243), R247 (= R251)
- binding tyrosine: Q39 (≠ I35), G40 (= G36), K104 (≠ R101), Y128 (= Y126), E130 (≠ I128), Y325 (= Y327), R367 (= R373)
Sites not aligning to the query:
6l1lB Apo-bacf structure from bacillus subtillis (see paper)
28% identity, 91% coverage: 28:392/399 of query aligns to 32:386/393 of 6l1lB
1o4sB Crystal structure of aspartate aminotransferase (tm1255) from thermotoga maritima at 1.90 a resolution (see paper)
25% identity, 91% coverage: 31:395/399 of query aligns to 40:383/384 of 1o4sB
- active site: W131 (≠ Y126), D209 (= D203), V211 (≠ C205), K242 (= K243)
- binding pyridoxal-5'-phosphate: G105 (= G99), A106 (≠ T100), K107 (≠ R101), W131 (≠ Y126), N181 (= N175), D209 (= D203), V211 (≠ C205), Y212 (= Y206), S241 (= S242), K242 (= K243), R250 (= R251)
1v2fA Crystal structure of t.Th hb8 glutamine aminotransferase complex with 3-phenylpropionate (see paper)
30% identity, 81% coverage: 31:355/399 of query aligns to 28:334/368 of 1v2fA
- active site: F112 (≠ Y126), A181 (≠ C193), A183 (≠ R195), K222 (= K243)
- binding hydrocinnamic acid: Q32 (≠ I35), G33 (= G36), Y57 (= Y60), F112 (≠ Y126), F253 (≠ C274)
- binding pyridoxal-5'-phosphate: G86 (= G99), A87 (≠ T100), T88 (≠ R101), F112 (≠ Y126), N163 (= N175), D191 (= D203), V193 (≠ C205), Y194 (= Y206), S219 (= S240), K222 (= K243), R230 (= R251)
Sites not aligning to the query:
1v2eA Crystal structure of t.Th hb8 glutamine aminotransferase complex with a-keto-g-methylthiobutyrate (see paper)
30% identity, 81% coverage: 31:355/399 of query aligns to 28:334/368 of 1v2eA
- active site: F112 (≠ Y126), A181 (≠ C193), A183 (≠ R195), K222 (= K243)
- binding 4-(methylsulfanyl)-2-oxobutanoic acid: Q32 (≠ I35), G33 (= G36), F112 (≠ Y126), K222 (= K243), F309 (≠ Y327)
- binding pyridoxal-5'-phosphate: G86 (= G99), A87 (≠ T100), T88 (≠ R101), F112 (≠ Y126), D191 (= D203), V193 (≠ C205), Y194 (= Y206), S219 (= S240), K222 (= K243), R230 (= R251)
Sites not aligning to the query:
1j32A Aspartate aminotransferase from phormidium lapideum
27% identity, 90% coverage: 36:395/399 of query aligns to 38:385/388 of 1j32A
- active site: W124 (≠ Y126), D202 (= D203), I204 (≠ C205), K237 (= K243)
- binding pyridoxal-5'-phosphate: G98 (= G99), G99 (≠ T100), K100 (≠ R101), W124 (≠ Y126), N174 (= N175), D202 (= D203), I204 (≠ C205), Y205 (= Y206), K237 (= K243), R245 (= R251)
P14909 Aspartate aminotransferase; AspAT; Transaminase A; EC 2.6.1.1 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see 3 papers)
23% identity, 92% coverage: 31:396/399 of query aligns to 38:397/402 of P14909
- K203 (≠ G197) modified: N6-methyllysine; partial
- K385 (≠ I384) modified: N6-methyllysine; partial
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
2zyjA Crystal structure of lysn, alpha-aminoadipate aminotransferase (complexed with n-(5'-phosphopyridoxyl)-l-glutamate), from thermus thermophilus hb27 (see paper)
27% identity, 80% coverage: 48:365/399 of query aligns to 58:366/397 of 2zyjA
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: G99 (= G99), S100 (≠ T100), Q101 (≠ R101), Y125 (= Y126), N174 (= N175), D202 (= D203), Y205 (= Y206), S235 (= S240), S237 (= S242), K238 (= K243), R245 (= R251)
Sites not aligning to the query:
Q72LL6 2-aminoadipate transaminase; 2-aminoadipate aminotransferase; Alpha-aminoadipate aminotransferase; AAA-AT; AadAT; EC 2.6.1.39 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see 2 papers)
27% identity, 80% coverage: 48:365/399 of query aligns to 58:366/397 of Q72LL6
- Y70 (= Y60) binding pyridoxal 5'-phosphate
- N174 (= N175) binding pyridoxal 5'-phosphate; binding substrate
- R245 (= R251) binding pyridoxal 5'-phosphate
Sites not aligning to the query:
- 20 S→E: Strongly decreases the affinity for AAA and Glu. A mild decrease of affinity is observed for 2-oxoglutarate. Increases the affinity for leucine and 2-oxoisocaproate.
- 23 R→A: Strongly decreases the affinity for AAA and Glu. A mild decrease of affinity is observed for 2-oxoglutarate which has the same chain length as Glu, but differs by the presence of a 2-oxo group which is not recognized by R-23. Increases the affinity for leucine and 2-oxoisocaproate due to the absence of gamma-carboxyl group.; R→Q: Strongly decreases the affinity for AAA and Glu. A mild decrease of affinity is observed for 2-oxoglutarate which has the same chain length as Glu, but differs by the presence of a 2-oxo group which is not recognized by R-23. Increases the affinity for leucine and 2-oxoisocaproate due to the absence of gamma-carboxyl group.
- 40 binding substrate
- 368 binding substrate
3cbfA Crystal structure of lysn, alpha-aminoadipate aminotransferase, from thermus thermophilus hb27 (see paper)
27% identity, 80% coverage: 48:365/399 of query aligns to 54:362/392 of 3cbfA
- binding (2S)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]hexanedioic acid: G95 (= G99), S96 (≠ T100), Q97 (≠ R101), Y121 (= Y126), N170 (= N175), D198 (= D203), Y201 (= Y206), S231 (= S240), S233 (= S242), K234 (= K243), R241 (= R251)
Sites not aligning to the query:
2egyA Crystal structure of lysn, alpha-aminoadipate aminotransferase (substrate free form), from thermus thermophilus hb27
27% identity, 80% coverage: 48:365/399 of query aligns to 54:362/392 of 2egyA
- binding pyridoxal-5'-phosphate: G95 (= G99), S96 (≠ T100), Q97 (≠ R101), Y121 (= Y126), N170 (= N175), D198 (= D203), A200 (≠ C205), Y201 (= Y206), S231 (= S240), S233 (= S242), K234 (= K243), R241 (= R251)
2z1yA Crystal structure of lysn, alpha-aminoadipate aminotransferase (complexed with n-(5'-phosphopyridoxyl)-l-leucine), from thermus thermophilus hb27
27% identity, 81% coverage: 41:365/399 of query aligns to 34:358/389 of 2z1yA
- binding leucine: Y117 (= Y126)
- binding pyridoxal-5'-phosphate: G91 (= G99), S92 (≠ T100), Q93 (≠ R101), Y117 (= Y126), N166 (= N175), D194 (= D203), Y197 (= Y206), S227 (= S240), S229 (= S242), K230 (= K243), R237 (= R251)
Sites not aligning to the query:
4wljA High resolution crystal structure of human kynurenine aminotransferase-i in complex with aminooxyacetate (see paper)
24% identity, 91% coverage: 31:393/399 of query aligns to 28:409/413 of 4wljA
- active site: F122 (≠ Y126), D204 (= D203), V206 (≠ C205), K238 (= K243)
- binding 4'-deoxy-4'-acetylyamino-pyridoxal-5'-phosphate: G96 (= G99), G97 (≠ T100), Y98 (≠ R101), F122 (≠ Y126), N172 (= N171), N176 (= N175), D204 (= D203), V206 (≠ C205), Y207 (= Y206), S235 (= S240), K238 (= K243), K246 (≠ R251), R389 (= R373)
Sites not aligning to the query:
1wstA Crystal structure of multiple substrate aminotransferase (msat) from thermococcus profundus
26% identity, 72% coverage: 34:321/399 of query aligns to 37:319/403 of 1wstA
- binding pyridoxal-5'-phosphate: G102 (= G99), S103 (≠ T100), Q104 (≠ R101), Y128 (= Y126), V177 (≠ L170), N182 (= N175), D210 (= D203), P212 (≠ C205), Y213 (= Y206), T240 (≠ S240), S242 (= S242), K243 (= K243), R250 (= R251)
Query Sequence
>WP_011814258.1 NCBI__GCF_000015585.1:WP_011814258.1
MNRGLSELQPYPFERMERLLTGRRAGPAAPIRLGIGEPQDPPPELLTRVLRDNLDQVARY
PATRGLESLREAIAGWLRRRYRLPEAAINPDEHVLPVAGTREALFAIAQTVIGRGRPYVA
MPNPFYQIYEGAALLSGARPLYLPIEPGSGLPDLDAIEPRTWQRVQLLYLNSPANPTGAV
ADSDYYRRVLELCDRYGFIIAADECYSEIYADEANPPPGLLQVCQAEGRADFHRCLVFHS
LSKRSNAPGLRSGFVAGDPGLIHAFLRYRTYQGCALPLHVQAASAAAWRDEAHVIENRAR
YRERYEEVCTILAEALDEVAPPPATFYLWPRTPIDDQTFTRRLWEEENVTVLPGSFLSRP
GGDGTPPGAGRIRMALVPDLATCIEAAERIRRFVHRNFQ
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory