SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011814259.1 NCBI__GCF_000015585.1:WP_011814259.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 7 hits to proteins with known functional sites (download)

3tdtA Complex of tetrahydrodipicolinate n-succinyltransferase with 2-amino- 6-oxopimelate and coenzyme a (see paper)
70% identity, 99% coverage: 4:272/273 of query aligns to 3:271/274 of 3tdtA

query
sites
3tdtA

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active site: D141 (= D142), G166 (= G167), E189 (= E190)
binding 2-amino-6-oxopimelic acid: R104 (= R105), M124 (= M125), M139 (= M140), D141 (= D142)
binding coenzyme a: G185 (= G186), A186 (= A187), S203 (= S204), M204 (= M205), R217 (= R218), V234 (= V235), K254 (= K255), K259 (= K260), T260 (= T261), K263 (= K264)

2tdtA Complex of tetrahydrodipicolinate n-succinyltransferase with 2- aminopimelate and coenzyme a (see paper)
70% identity, 99% coverage: 4:272/273 of query aligns to 3:271/274 of 2tdtA

query
sites
2tdtA

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active site: D141 (= D142), G166 (= G167), E189 (= E190)
binding coenzyme a: S159 (= S160), F183 (= F184), G185 (= G186), S203 (= S204), M204 (= M205), R217 (= R218), K254 (= K255), K259 (= K260), T260 (= T261), K263 (= K264)
binding (2S)-2-aminoheptanedioic acid: R104 (= R105), M124 (= M125), M139 (= M140), D141 (= D142)

1kgtA Crystal structure of tetrahydrodipicolinate n-succinyltransferase in complex with pimelate and succinyl-coa (see paper)
70% identity, 99% coverage: 4:272/273 of query aligns to 3:271/274 of 1kgtA

query
sites
1kgtA

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active site: D141 (= D142), G166 (= G167), E189 (= E190)
binding pimelic acid: R104 (= R105), M124 (= M125), M139 (= M140)
binding succinyl-coenzyme a: D141 (= D142), S159 (= S160), A186 (= A187), S203 (= S204), R217 (= R218), K259 (= K260), T260 (= T261), K263 (= K264)

1kgqA Crystal structure of tetrahydrodipicolinate n-succinyltransferase in complex with l-2-aminopimelate and succinamide-coa (see paper)
70% identity, 99% coverage: 4:272/273 of query aligns to 3:271/274 of 1kgqA

query
sites
1kgqA

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active site: D141 (= D142), G166 (= G167), E189 (= E190)
binding (2S)-2-aminoheptanedioic acid: R104 (= R105), M124 (= M125), M139 (= M140), D141 (= D142)
binding succinamide-coa: F183 (= F184), G185 (= G186), S203 (= S204), M204 (= M205), R217 (= R218), V234 (= V235), K254 (= K255), K259 (= K260), T260 (= T261), K263 (= K264)

3r8yA Structure of the bacillus anthracis tetrahydropicolinate succinyltransferase
48% identity, 28% coverage: 122:198/273 of query aligns to 95:171/203 of 3r8yA

query
sites
3r8yA

V

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x
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active site: D115 (= D142), G140 (= G167), V163 (≠ E190)
binding calcium ion: G121 (= G148), G122 (≠ S149)

3r5bA Pseudomonas aeruginosa dapd (pa3666) in complex with l-2-aminopimelate (see paper)
27% identity, 51% coverage: 76:215/273 of query aligns to 155:284/321 of 3r5bA

query
sites
3r5bA

M

L

R

K

G

G

G

K

V

L

T

L

N

E

F

V

F

F

-

S

-

V

D

D

K

K

V

F

P

P

M

K

K

M

F

-

A

-

G

-

W

-

G

-

E

T

D

D

A

Y

L

V

R

V

E

P

S

A

G

G

V

V

R

R

V

I

V

A

P

D

P

T

A

A

A

R

A

V

R
|
R

R

L

G

G

S

A

Y

Y

I

I

A

G

P

E

G

G

V

T

V

T

L

V

M

M

-

H

P

E

S

G

Y

F

V

V

N

N

I

F

G

N

A

A

Y

G

V

T

D

E

E

G

G

P

T

G

M

M

V

I

D

E

T

G

W

R

A

V

T
x
S

V

A

G

G

S

V

C

F

A

-

Q

-

I

V

G

G

K

K

N

G

V

S

H

D

L

L

S

G

G

G

G

G

V

C

G

S

I

T

G

M

G

G

V

T

L

L

E

N

P

I

L

V

Q

I

A

S

N

-

P

-

T

-

I

-

I

V

E

G

D

E

D

G

C

C

F

L

I

I

G

G

A

A

R

N

S

A

E

G

I

I

V

-

E

-

G

G

V

I

T

P

V

L

G

G

R

D

G

R

A

N

V

I

I

V

S

E

M

A

G

G

V

L

Y

Y

V

I

G

T

Q

A

S

G

T

T

K

K

I

V

binding (2S)-2-aminoheptanedioic acid: R189 (= R113), S223 (≠ T146)

Sites not aligning to the query:

binding (2S)-2-aminoheptanedioic acid: 127

3r5aA Pseudomonas aeruginosa dapd (pa3666) in complex with d-2-aminopimelate (see paper)
27% identity, 51% coverage: 76:215/273 of query aligns to 155:284/321 of 3r5aA

query
sites
3r5aA

M

L

R

K

G

G

G

K

V

L

T

L

N

E

F

V

F

F

-

S

-

V

D

D

K

K

V

F

P

P

M

K

K

M

F

-

A

-

G

-

W

-

G

-

E

T

D

D

A

Y

L

V

R

V

E

P

S

A

G

G

V

V

R
|
R

V

I

V

A

P

D

P

T

A

A

A

R

A

V

R
|
R

R

L

G

G

S

A

Y

Y

I

I

A

G

P

E

G

G

V

T

V

T

L

V

M
|
M

-

H

P

E

S

G

Y

F

V

V

N
|
N

I

F

G

N

A

A

Y

G

V

T

D

E

E

G

G

P

T

G

M
|
M

V

I

D

E

T

G

W

R

A

V

T
x
S

V
x
A

G

G

S

V

C

F

A

-

Q

-

I

V

G

G

K

K

N

G

V

S

H

D

L

L

S

G

G

G

G

G

V

C

G

S

I

T

G

M

G

G

V

T

L

L

E

N

P

I

L

V

Q

I

A

S

N

-

P

-

T

-

I

-

I

V

E

G

D

E

D

G

C

C

F

L

I

I

G

G

A

A

R

N

S

A

E

G

I

I

V

-

E

-

G

G

V

I

T

P

V

L

G

G

R

D

G

R

A

N

V

I

I

V

S

E

M

A

G

G

V

L

Y

Y

V

I

G

T

Q

A

S

G

T

T

K

K

I

V

binding (2R)-2-aminoheptanedioic acid: R181 (= R105), R189 (= R113), M201 (= M125), N207 (= N130), M217 (= M140), S223 (≠ T146), A224 (≠ V147)

Sites not aligning to the query:

binding (2R)-2-aminoheptanedioic acid: 127

Query Sequence

>WP_011814259.1 NCBI__GCF_000015585.1:WP_011814259.1
MAAQIQKVIDEAFEQRAQLNPNEAPAEIREAVEEALRRLDSGQARVAEPGADGWQVNEWL
KKAVLLSFRLNDNRLMRGGVTNFFDKVPMKFAGWGEDALRESGVRVVPPAAARRGSYIAP
GVVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEPLQANPTIIE
DDCFIGARSEIVEGVTVGRGAVISMGVYVGQSTKIYDRTTGEVHFGHVPEGAVVVPGNLP
AADGSHSLYCAVIIKYADEKTRAKVGINELLRP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory