SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011814939.1 NCBI__GCF_000015585.1:WP_011814939.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7kb1C Complex of o-acety-l-homoserine aminocarboxypropyltransferase (mety) from thermotoga maritima and a key reaction intermediate (see paper)
56% identity, 99% coverage: 5:421/422 of query aligns to 9:424/428 of 7kb1C

query
sites
7kb1C

T

T

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R

A

A

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H

H

A

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Y

Y

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D

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H

-

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C

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M

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F

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N

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A

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G

G

I

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E

T

T

L

L

P

S

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K

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I

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D

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A

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N

I
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I

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D

A

A

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T

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A

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C

I

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H

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E

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A

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R

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binding pyridoxal-5'-phosphate: Y57 (= Y52), R59 (= R54)
binding (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)imino]but-3-enoic acid: G87 (= G82), Q88 (≠ M83), Y112 (= Y107), N160 (= N154), D185 (= D179), S206 (= S201), T208 (= T203), K209 (= K204), N369 (= N366), I370 (= I367), R404 (= R401)

7kb1A Complex of o-acety-l-homoserine aminocarboxypropyltransferase (mety) from thermotoga maritima and a key reaction intermediate (see paper)
56% identity, 99% coverage: 5:421/422 of query aligns to 9:424/428 of 7kb1A

query
sites
7kb1A

T

T

Q

R

A

A

V

L

H

H

A

A

G

G

Y

Y

S

E

P

P

-

P

D

E

P

Q

T

A

T

T

R

G

A

S

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R

A

A

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Y

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S

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Y

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F

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Q

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A

A

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E

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G

I

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G

G

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A

L

L

A

A

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A

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x
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A

A

A

A

I

I

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Y

A

A

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L

A

N

I

I

T

A

R

G

A

P

G

G

D

D

N

E

I

I

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S

R

A

L

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Y

G

G

G

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T

T

Y

Y

N

N

L

L

F

F

A

R

H

H

S

T

L

L

-

Y

P

K

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K

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G

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I

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I

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F

F

A

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E

D

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D

D

Y

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L

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A

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G

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E

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T

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S

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E

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A

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I

G

G

D

D

A

A

K

R

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T

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L

A

A

C

I

H

H

P

P

A

A

S

S

T

T

T

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H

H

R

Q

Q

Q

L

L

N

T

D

E

E

E

E

E

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A

T

G

G

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D

D

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R

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G

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E

E

H

D

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E

D

D

I

I

L

I

A

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D

I

L

D

D

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A

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R

binding (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)imino]but-3-enoic acid: Y57 (= Y52), R59 (= R54), G87 (= G82), Q88 (≠ M83), Y112 (= Y107), N160 (= N154), D185 (= D179), S206 (= S201), T208 (= T203), K209 (= K204), N369 (= N366), I370 (= I367), R404 (= R401)

8erbK Crystal structure of fub7 in complex with vinylglycine ketimine (see paper)
53% identity, 100% coverage: 2:421/422 of query aligns to 5:425/429 of 8erbK

query
sites
8erbK

K

N

L

F

E

E

T

T

Q

L

A

Q

V

L

H

H

A

A

G

G

Y

Y

S

T

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D

D

P

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T

H

T

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R

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A

A

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P

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I

Y

Y

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S

Y

Y

A

T

F

F

D

N

D

D

T

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Q

A

H

H

G

G

A

A

D

R

L

L

F

F

D

G

L

L

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K

V

E

E

L

G

G

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N

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Y

T

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I

L

M

M

N

N

P

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T

T

N

V

A

D

V

V

L

F

E

E

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A

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L

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E

G

G

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I

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G

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A

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M
x
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A

A

A

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x
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T

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Y

L

A

T

I

I

Q

A

A

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I

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T

A

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K

A

A

G

G

D

D

N

N

I

I

V

V

S

A

T

S

S

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R

H

L

L

Y
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Y

G

G

G

G

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T

Y

Y

N

N

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F

L

A

N

H

V

S

L

L

L

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I

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H

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G

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I

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F

F

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A

A

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A

I

I

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T

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R

A

A

L

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E

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S

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E

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D
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D

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T

A

-

G

P

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Y

Y

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S

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K
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K

Y

W

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I

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H

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I

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F

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A

A

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C

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F

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A

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R

L

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V

G

G

L

I

E

E

H

H

V

V

D

D

D

D

I

I

L

I

A

A

D

D

I

F

D

E

Q

Q

A

S

L

F

Q

Q

binding (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)imino]but-3-enoic acid: Y55 (= Y52), R57 (= R54), G85 (= G82), Q86 (≠ M83), Q89 (≠ I86), Y110 (= Y107), N157 (= N154), D182 (= D179), S205 (= S201), T207 (= T203), K208 (= K204), T385 (= T381), R405 (= R401)

8erjB Crystal structure of fub7 in complex with e-2-aminocrotonate (see paper)
53% identity, 100% coverage: 2:421/422 of query aligns to 4:424/428 of 8erjB

query
sites
8erjB

K

N

L

F

E

E

T

T

Q

L

A

Q

V

L

H

H

A

A

G

G

Y

Y

S

T

P

P

D

D

P

P

T

H

T

T

R

R

A

S

V

T

A

A

V

V

P

P

I

I

Y

Y

Q

A

T

T

T

S

S

S

Y

Y

A

T

F

F

D

N

D

D

T

S

Q

A

H

H

G

G

A

A

D

R

L

L

F

F

D

G

L

L

K

K

V

E

E

L

G

G

N

N

I

I

Y

Y

T

S

R

R

I

L

M

M

N

N

P

P

T

T

N

V

A

D

V

V

L

F

E

E

Q

K

R

R

V

I

A

A

A

A

M

L

E

E

G

G

G

G

I

I

G

A

G

A

L

A

A

A

V

T

A

S

S

S

G
|
G

M
x
Q

A

A

A

A

I
x
Q

T

F

Y

L

A

T

I

I

Q

A

A

T

I

L

T

A

R

K

A

A

G

G

D

D

N

N

I

I

V

V

S

A

T

S

S

S

R

H

L

L

Y
|
Y

G

G

G

G

T

T

Y

Y

N

N

L

Q

F

L

A

N

H

V

S

L

L

L

P

P

I

R

H

F

G

G

I

I

E

K

V

T

R

K

F

F

A

V

A

R

Q

S

D

G

D

K

Y

L

E

E

R

D

L

Y

E

A

A

A

Q

A

I

I

D

D

E

D

R

Q

T

T

K

R

A

A

L

I

F

Y

C

V

E

E

S

S

V

M

G

S

N

N

P

P

S

D

G

Y

E

V

V

V

V

P

D

D

V

F

E

E

R

G

L

I

A

A

E

K

I

I

A

A

H

H

R

E

H

H

G

G

I

I

P

P

L

L

M

V

V

V

D
|
D

N

N

T

T

V

L

P

G

T

A

-

G

P

G

Y

Y

L

Y

W

I

R

R

P

P

I

I

D

E

N

H

G

G

A

A

D

D

I

I

V

V

I

V

H

H

S
|
S

L

A

T

T

K
|
K

Y

W

M

I

G

G

G

G

H

H

G

G

T

T

T

T

V

I

G

G

G

G

V

V

I

I

V

V

D

D

S

S

G

G

K

R

F

F

P

N

W

W

A

N

D

K

H

H

G

S

D

D

R

R

F

F

P

P

M

E

M

M

V

V

E

E

P

P

D

S

P

P

S

S

Y

Y

H

H

G

G

V

L

V

K

Y

Y

T

W

E

E

D

A

M

F

G

G

E

P

A

A

A

T

Y

F

I

I

T

T

R

R

C

I

R

R

V

V

V

E

P

M

L

L

R

R

N

D

M

I

G

G

A

A

A

C

L

L

S

S

P

P

F

F

N

S

A

A

F

Q

Q

Q

L

L

L

L

Q

L

G

G

I

I

E

E

T

T

L

L

P

G

V

L

R

R

M

A

D

E

R

R

H

H

C

A

D

Q

N

N

A

T

Q

E

R

K

V

L

A

S

E

K

Y

Y

L

F

Q

E

A

S

H

S

P

P

R

N

V

V

S

S

W

W

V

V

K

L

F

W

A

P

G

G

L

S

E

E

D

S

S

H

P

P

Y

T

K

Y

P

S

L

Q

A

A

D

K

R

K

Y

Y

M

L

G

T

G

R

R

G

A

F

A

G

S

A

I

M

L

L

S

S

F

I

G

G

V

V

Q

K

G

G

G

D

F

A

E

S

A

A

G

G

A

S

R

K

F

V

I

V

D

D

A

G

L

L

Q

K

L

L

I

V

T

S

R

N

L

L

V
x
A

N
|
N

I

V

G

G

D

D

A

A

K

K

S

S

L

L

A

A

C

I

H

H

P

P

A

W

S

S

T

T

T
|
T

H

H

R

E

Q

Q

L

L

N

S

D

E

E

D

E

E

L

R

E

L

S

A

A

S

G

G

V

V

S

T

R

E

D

D

L

M

V

I

R
|
R

L

I

S

S

I

V

G

G

L

I

E

E

H

H

V

V

D

D

D

D

I

I

L

I

A

A

D

D

I

F

D

E

Q

Q

A

S

L

F

Q

Q

binding (2S)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]but-3-enoic acid: G84 (= G82), Q85 (≠ M83), Q88 (≠ I86), Y109 (= Y107), D181 (= D179), S204 (= S201), K207 (= K204), A368 (≠ V365), N369 (= N366), T384 (= T381), R404 (= R401)

8erjA Crystal structure of fub7 in complex with e-2-aminocrotonate (see paper)
53% identity, 100% coverage: 2:421/422 of query aligns to 4:424/428 of 8erjA

query
sites
8erjA

K

N

L

F

E

E

T

T

Q

L

A

Q

V

L

H

H

A

A

G

G

Y

Y

S

T

P

P

D

D

P

P

T

H

T

T

R

R

A

S

V

T

A

A

V

V

P

P

I

I

Y

Y

Q

A

T

T

T

S

S

S

Y

Y

A

T

F

F

D

N

D

D

T

S

Q

A

H

H

G

G

A

A

D

R

L

L

F

F

D

G

L

L

K

K

V

E

E

L

G

G

N

N

I

I

Y

Y

T

S

R

R

I

L

M

M

N

N

P

P

T

T

N

V

A

D

V

V

L

F

E

E

Q

K

R

R

V

I

A

A

A

A

M

L

E

E

G

G

G

G

I

I

G

A

G

A

L

A

A

A

V

T

A

S

S
|
S

G
|
G

M
x
Q

A

A

A

A

I
x
Q

T

F

Y

L

A

T

I

I

Q

A

A

T

I

L

T

A

R

K

A

A

G

G

D

D

N

N

I

I

V

V

S

A

T

S

S

S

R

H

L

L

Y
|
Y

G

G

G

G

T

T

Y

Y

N

N

L

Q

F

L

A

N

H

V

S

L

L

L

P

P

I

R

H

F

G

G

I

I

E

K

V

T

R

K

F

F

A

V

A

R

Q

S

D

G

D

K

Y

L

E

E

R

D

L

Y

E

A

A

A

Q

A

I

I

D

D

E

D

R

Q

T

T

K

R

A

A

L

I

F

Y

C

V

E

E

S

S

V

M

G

S

N
|
N

P

P

S

D

G

Y

E

V

V

V

V

P

D

D

V

F

E

E

R

G

L

I

A

A

E

K

I

I

A

A

H

H

R

E

H

H

G

G

I

I

P

P

L

L

M

V

V

V

D
|
D

N

N

T

T

V

L

P

G

T

A

-

G

P

G

Y

Y

L

Y

W

I

R

R

P

P

I

I

D

E

N

H

G

G

A

A

D

D

I

I

V

V

I

V

H

H

S
|
S

L

A

T
|
T

K
|
K

Y

W

M

I

G

G

G

G

H

H

G

G

T

T

T

T

V

I

G

G

G

G

V

V

I

I

V

V

D

D

S

S

G

G

K

R

F

F

P

N

W

W

A

N

D

K

H

H

G

S

D

D

R

R

F

F

P

P

M

E

M

M

V

V

E

E

P

P

D

S

P

P

S

S

Y

Y

H

H

G

G

V

L

V

K

Y

Y

T

W

E

E

D

A

M

F

G

G

E

P

A

A

A

T

Y

F

I

I

T

T

R

R

C

I

R

R

V

V

V

E

P

M

L

L

R

R

N

D

M

I

G

G

A

A

A

C

L

L

S

S

P

P

F

F

N

S

A

A

F

Q

Q

Q

L

L

L

L

Q

L

G

G

I

I

E

E

T

T

L

L

P

G

V

L

R

R

M

A

D

E

R

R

H

H

C

A

D

Q

N

N

A

T

Q

E

R

K

V

L

A

S

E

K

Y

Y

L

F

Q

E

A

S

H

S

P

P

R

N

V

V

S

S

W

W

V

V

K

L

F

W

A

P

G

G

L

S

E

E

D

S

S

H

P

P

Y

T

K

Y

P

S

L

Q

A

A

D

K

R

K

Y

Y

M

L

G

T

G

R

R

G

A

F

A

G

S

A

I

M

L

L

S

S

F

I

G

G

V

V

Q

K

G

G

G

D

F

A

E

S

A

A

G

G

A

S

R

K

F

V

I

V

D

D

A

G

L

L

Q

K

L

L

I

V

T

S

R

N

L

L

V

A

N

N

I

V

G

G

D

D

A

A

K

K

S

S

L

L

A

A

C

I

H

H

P

P

A

W

S

S

T

T

T

T

H

H

R

E

Q

Q

L

L

N

S

D

E

E

D

E

E

L

R

E

L

S

A

A

S

G

G

V

V

S

T

R

E

D

D

L

M

V

I

R
|
R

L

I

S

S

I

V

G

G

L

I

E

E

H

H

V

V

D

D

D

D

I

I

L

I

A

A

D

D

I

F

D

E

Q

Q

A

S

L

F

Q

Q

binding (2E)-2-{[(1E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}but-2-enoic acid: S83 (= S81), G84 (= G82), Q85 (≠ M83), Q88 (≠ I86), Y109 (= Y107), N156 (= N154), D181 (= D179), S204 (= S201), T206 (= T203), K207 (= K204), R404 (= R401)

O13326 Homocysteine synthase; O-acetylhomoserine sulfhydrylase; OAH SHL; OAH sulfhydrylase; EC 2.5.1.49 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
54% identity, 99% coverage: 3:421/422 of query aligns to 8:427/429 of O13326

query
sites
O13326

L

F

E

E

T

T

Q

L

A

Q

V

L

H

H

A

A

G

G

Y

Q

S

E

P

P

D

D

P

A

T

A

T

T

R

S

A

S

V

R

A

A

V

V

P

P

I

I

Y

Y

Q

A

T

T

T

T

S

S

Y

Y

A

V

F

F

D

R

D

D

T

C

Q

D

H

H

G

G

A

G

D

R

L

L

F

F

D

G

L

L

K

Q

V

E

E

P

G

G

N

Y

I

I

Y

Y

T

S

R

R

I

M

M

M

N

N

P

P

T

T

N

A

A

D

V

V

L

F

E

E

Q

K

R

R

V

I

A

A

A

A

M

L

E

E

G

H

G

G

I

A

G

A

G

A

L

I

A

A

V

T

A

S

S

S

G

G

M

T

A

S

A

A

I

L

T

F

Y

M

A

A

I

L

Q

T

A

T

I

L

T

A

R

K

A

A

G

G

D

D

N

N

I

I

V

V

S

S

T

T

S

S

R

Y

L

L

Y

Y

G

G

G

G

T

T

Y

Y

N

N

L

L

F

F

A

K

H

V

S

T

L

L

P

P

I

R

H

L

G

G

I

I

E

T

V

T

R

K

F

F

A

V

A

N

Q

G

D

D

D

D

Y

P

E

N

R

D

L

L

E

A

A

A

Q

Q

I

I

D

D

E

E

R

N

T

T

K

K

A

A

L

V

F

Y

C

V

E

E

S

S

V

I

G

G

N

N

P

P

S

M

G

Y

E

N

V

V

V

P

D

D

V

F

E

E

R

R

L

I

A

A

E

E

I

V

A

A

H

H

R

A

H

A

G

G

I

V

P

P

L

L

M

M

V

V

D

D

N

N

T

T

V

F

-

G

P

G

T

G

P

G

Y

Y

L

L

W

V

R

R

P

P

I

I

D

D

N

H

G

G

A

A

D

D

I

I

V

V

I

T

H

H

S

S

L

A

T

T

K

K

Y

W

M

I

G

G

G

G

H

H

G

G

T

T

T

T

V

I

G

G

G

G

V

V

I

I

V

V

D

D

S

S

G

G

K

K

F

F

P

D

W

W

A

K

D

K

H

N

G

S

D

K

R

R

F

F

P

P

M

E

M

F

V

N

E

E

P

P

D

H

P

P

S

G

Y

Y

H

H

G

G

V

M

V

V

Y

F

T

T

E

E

D

T

M

F

G

G

E

N

A

L

A

A

Y

Y

I

A

T

F

R

A

C

C

R

R

V

T

V

Q

P

T

L

L

R

R

N

D

M

V

G

G

A

G

A

N

L

A

S

N

P

P

F

F

N

G

A

V

F

F

Q

L

L

L

L

L

Q

Q

G

G

I

L

E

E

T

T

L

L

P

S

V

L

R

R

M

M

D

E

R

R

H

H

C

V

D

Q

N

N

A

A

Q

F

R

A

V

L

A

A

E

K

Y

Y

L

L

Q

E

A

K

H

H

P

P

R

K

V

V

S

N

W

W

V

V

K

S

F

Y

A

P

G

G

L

L

E

E

D

S

S

H

P

V

Y

S

K

H

P

K

L

L

A

A

D

K

R

K

Y

Y

M

L

G

K

G

N

R

G

A

Y

A

G

S

A

I

V

L

L

S

S

F

F

G

G

V

A

Q

K

G

G

G

G

F

P

E

D

A

Q

G

S

A

R

R

K

F

V

I

V

D

N

A

A

L

L

Q

K

L

L

I

A

T

S

R

Q

L

L

V

A

N

N

I

V

G

G

D

D

A

A

K

K

S

T

L

L

A

V

C

I

H

A

P

P

A

A

S

Y

T

T

T

T

H

H

R

L

Q

Q

L

L

N

T

D

D

E

E

E

E

L

Q

E

I

S

S

A

A

G

G

V

V

S

T

R

K

D

D

L

L

V

I

R

R

L

V

S

A

I

V

G
|
G

L

I

E

E

H

H

V

I

D

D

D

D

I

I

L

I

A

A

D

D

I

F

D

A

Q

Q

A

A

L

L

Q

E

G411 (= G405) mutation to D: Impairs homocysteine synthase activity.

2ctzA Crystal structure of o-acetyl homoserine sulfhydrylase from thermus thermophilus hb8
51% identity, 100% coverage: 1:420/422 of query aligns to 1:420/421 of 2ctzA

query
sites
2ctzA

M

M

K

R

L

F

E

E

T

T

Q

L

A

Q

V

L

H

H

A

A

G

G

Y

Y

S

E

P

P

D

E

P

P

T

T

T

T

R

L

A

S

V

R

A

Q

V

V

P

P

I

I

Y

Y

Q

P

T

T

T

T

S

S

Y

Y

A

V

F

F

D

K

D

S

T

P

Q

E

H

H

G

A

A

A

D

N

L

L

F

F

D

A

L

L

K

K

V

E

E

F

G

G

N

N

I

I

Y

Y

T

S

R
|
R

I

I

M

M

N

N

P

P

T

T

N

V

A

D

V

V

L

L

E

E

Q

K

R

R

V

L

A

A

A

A

M

L

E

E

G

G

G

G

I

K

G

A

G

A

L

L

A

A

V

T

A

A

S
|
S

G
|
G

M
x
H

A

A

A

A

I
x
Q

T

F

Y

L

A

A

I

L

Q

T

A

T

I

L

T

A

R

Q

A

A

G

G

D

D

N

N

I

I

V

V

S

S

T

T

S

P

R

N

L

L

Y
|
Y

G

G

G

G

T

T

Y

F

N

N

L

Q

F

F

A

K

H

V

S

T

L

L

P

K

I

R

H

L

G

G

I

I

E

E

V

V

R

R

F

F

A

T

A

S

Q

R

D

E

D

E

Y

R

-

P

E

E

R

E

L

F

E

L

A

A

Q

L

I

T

D

D

E

E

R

K

T

T

K

R

A

A

L

W

F

W

C

V

E

E

S

S

V

I

G

G

N

N

P

P

S

A

G

L

E

N

V

I

V

P

D

D

V

L

E

E

R

A

L

L

A

A

E

Q

I

A

A

A

H

R

R

E

H

K

G

G

I

V

P

A

L

L

M

I

V

V

D
|
D

N

N

T
|
T

V

F

P

G

T

M

P

G

-

G

Y

Y

L

L

W

L

R

R

P

P

I

L

D

A

N

W

G

G

A

A

D

A

I

L

V

V

I

T

H

H

S
|
S

L

L

T
|
T

K
|
K

Y

W

M

V

G

G

G

G

H

H

G

G

T

A

T

V

V

I

G

A

G

G

V

A

I

I

V

V

D

D

S

G

G

G

K

N

F

F

P

P

W

W

A

-

D

-

H

E

G

G

D

G

R

R

F

Y

P

P

M

L

M

L

V

T

E

E

P

P

D

Q

P

P

S

G

Y

Y

H

H

G

G

V

L

V

R

Y

L

T

T

E

E

D

A

M

F

G

G

E

E

A

L

A

A

Y

F

I

I

T

V

R

K

C

A

R

R

V

V

V

D

P

G

L

L

R

R

N

D

M

Q

G

G

A

Q

A

A

L

L

S

G

P

P

F

F

N

E

A

A

F

W

Q

V

L

V

L

L

Q

L

G

G

I

M

E

E

T

T

L

L

P

S

V

L

R

R

M

A

D

E

R

R

H

H

C

V

D

E

N

N

A

T

Q

L

R

H

V

L

A

A

E

H

Y

W

L

L

Q

L

A

E

H

Q

P

P

R

Q

V

V

S

A

W

W

V

V

K

N

F

Y

A

P

G

G

L

L

E

P

D

H

S

H

P

P

Y

H

K

H

P

D

L

R

A

A

D

Q

R

K

Y

Y

M

F

G

K

G

G

R

K

A

P

A

G

S

A

I

V

L

L

S

T

F

F

G

G

V

L

Q

K

G

G

G

G

F

Y

E

E

A

A

G

A

A

K

R

R

F

F

I

I

D

S

A

R

L

L

Q

K

L

L

I

I

T

S

R

H

L

L

V

A

N

N

I

V

G

G

D

D

A

T

K

R

S

T

L

L

A

A

C

I

H

H

P

P

A

A

S

S

T

T

T

T

H

H

R

S

Q

Q

L

L

N

S

D

P

E

E

E

E

L

Q

E

A

S

Q

A

A

G

G

V

V

S

S

R

P

D

E

L

M

V

V

R

R

L

L

S

S

I

V

G

G

L

L

E

E

H

H

V

V

D

E

D

D

I

L

L

K

A

A

D

E

I

L

D

K

Q

E

A

A

L

L

active site: R54 (= R54), Y107 (= Y107), D180 (= D179), K206 (= K204)
binding pyridoxal-5'-phosphate: S81 (= S81), G82 (= G82), H83 (≠ M83), Q86 (≠ I86), Y107 (= Y107), D180 (= D179), T182 (= T181), S203 (= S201), T205 (= T203), K206 (= K204)

Q5SK88 O-acetyl-L-homoserine sulfhydrylase 1; OAH-sulfhydrylase 1; EC 2.5.1.- from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
51% identity, 100% coverage: 1:420/422 of query aligns to 1:420/421 of Q5SK88

query
sites
Q5SK88

M

M

K

R

L

F

E

E

T

T

Q

L

A

Q

V

L

H

H

A

A

G

G

Y

Y

S

E

P

P

D

E

P

P

T

T

T

T

R

L

A

S

V

R

A

Q

V

V

P

P

I

I

Y

Y

Q

P

T

T

T

T

S

S

Y

Y

A

V

F

F

D

K

D

S

T

P

Q

E

H

H

G

A

A

A

D

N

L

L

F

F

D

A

L

L

K

K

V

E

E

F

G

G

N

N

I

I

Y

Y

T

S

R

R

I

I

M

M

N

N

P

P

T

T

N

V

A

D

V

V

L

L

E

E

Q

K

R

R

V

L

A

A

A

A

M

L

E

E

G

G

G

G

I

K

G

A

G

A

L

L

A

A

V

T

A

A

S

S

G

G

M

H

A

A

A

A

I

Q

T

F

Y

L

A

A

I

L

Q

T

A

T

I

L

T

A

R

Q

A

A

G

G

D

D

N

N

I

I

V

V

S

S

T

T

S

P

R

N

L

L

Y

Y

G

G

G

G

T

T

Y

F

N

N

L

Q

F

F

A

K

H

V

S

T

L

L

P

K

I

R

H

L

G

G

I

I

E

E

V

V

R

R

F

F

A

T

A

S

Q

R

D

E

D

E

Y

R

-

P

E

E

R

E

L

F

E

L

A

A

Q

L

I

T

D

D

E

E

R

K

T

T

K

R

A

A

L

W

F

W

C

V

E

E

S

S

V

I

G

G

N

N

P

P

S

A

G

L

E

N

V

I

V

P

D

D

V

L

E

E

R

A

L

L

A

A

E

Q

I

A

A

A

H

R

R

E

H

K

G

G

I

V

P

A

L

L

M

I

V

V

D

D

N

N

T

T

V

F

P

G

T

M

P

G

-

G

Y

Y

L

L

W

L

R

R

P

P

I

L

D

A

N

W

G

G

A

A

D

A

I

L

V

V

I

T

H

H

S

S

L

L

T

T

K
|
K

Y

W

M

V

G

G

G

G

H

H

G

G

T

A

T

V

V

I

G

A

G

G

V

A

I

I

V

V

D

D

S

G

G

G

K

N

F

F

P

P

W

W

A

-

D

-

H

E

G

G

D

G

R

R

F

Y

P

P

M

L

M

L

V

T

E

E

P

P

D

Q

P

P

S

G

Y

Y

H

H

G

G

V

L

V

R

Y

L

T

T

E

E

D

A

M

F

G

G

E

E

A

L

A

A

Y

F

I

I

T

V

R

K

C

A

R

R

V

V

V

D

P

G

L

L

R

R

N

D

M

Q

G

G

A

Q

A

A

L

L

S

G

P

P

F

F

N

E

A

A

F

W

Q

V

L

V

L

L

Q

L

G

G

I

M

E

E

T

T

L

L

P

S

V

L

R

R

M

A

D

E

R

R

H

H

C

V

D

E

N

N

A

T

Q

L

R

H

V

L

A

A

E

H

Y

W

L

L

Q

L

A

E

H

Q

P

P

R

Q

V

V

S

A

W

W

V

V

K

N

F

Y

A

P

G

G

L

L

E

P

D

H

S

H

P

P

Y

H

K

H

P

D

L

R

A

A

D

Q

R

K

Y

Y

M

F

G

K

G

G

R

K

A

P

A

G

S

A

I

V

L

L

S

T

F

F

G

G

V

L

Q

K

G

G

G

G

F

Y

E

E

A

A

G

A

A

K

R

R

F

F

I

I

D

S

A

R

L

L

Q

K

L

L

I

I

T

S

R

H

L

L

V

A

N

N

I

V

G

G

D

D

A

T

K

R

S

T

L

L

A

A

C

I

H

H

P

P

A

A

S

S

T

T

T

T

H

H

R

S

Q

Q

L

L

N

S

D

P

E

E

E

E

L

Q

E

A

S

Q

A

A

G

G

V

V

S

S

R

P

D

E

L

M

V

V

R

R

L

L

S

S

I

V

G

G

L

L

E

E

H

H

V

V

D

E

D

D

I

L

L

K

A

A

D

E

I

L

D

K

Q

E

A

A

L

L

K206 (= K204) modified: N6-(pyridoxal phosphate)lysine

8wkoA Crystal structure of o-acetylhomoserine sulfhydrylase from lactobacillus plantarum in the closed form (see paper)
51% identity, 99% coverage: 3:420/422 of query aligns to 9:422/425 of 8wkoA

query
sites
8wkoA

L

F

E

E

T

T

Q

L

A

Q

V

V

H

H

A

A

G

G

Y

Q

S

T

P

V

D

D

P

E

T

T

T

-

R

G

A

A

V

R

A

A

V

V

P

P

I

I

Y

Y

Q

Q

T

T

T

T

S

S

Y

Y

A

V

F

F

D

K

D

D

T

A

Q

K

H

Q

G

A

A

A

D

G

L

R

F

F

D

A

L

L

K

T

V

D

E

A

G

G

N

N

I

I

Y

Y

T

T

R

R

I

L

M

T

N

N

P

P

T

T

N

T

A

D

V

V

L

V

E

D

Q

K

R

R

V

V

A

A

A

A

M

L

E

E

G

H

G

G

I

T

G

A

G

G

L

V

A

T

V

L

A

A

S

T

G
|
G

M
x
S

A

A

A

A

I

I

T

T

Y

A

A

A

I

I

Q

L

A

N

I

I

T

A

R

Q

A

Q

G

G

D

D

N

E

I

I

V

V

S

A

T

A

S

N

R

T

L

L

Y
|
Y

G

G

G

G

T

T

Y

Y

N

D

L

L

F

F

A

S

H

V

S

T

L

L

P

K

I

K

H

L

G

G

I

I

E

T

V

T

R

H

F

F

A

V

A

D

Q

P

D

D

D

E

Y

P

E

A

R

N

L

F

E

E

A

T

Q

A

I

I

D

N

E

D

R

H

T

T

K

K

A

A

L

L

F

Y

C

V

E
|
E

S

S

V

I

G

G

N

N

P

P

S

G

G

I

E

N

V

L

V

V

D

D

V

F

E

E

R

A

L

I

A

G

E

K

I

I

A

A

H

H

R

D

H

H

G

G

I

I

P

I

L

F

M

I

V

V

D
|
D

N

N

T
|
T

V

F

P

G

T

T

P

P

Y

Y

L

L

W

V

R

R

P

P

I

L

D

E

N

H

G

G

A

A

D

D

I

V

V

V

I

V

H

H

S
|
S

L
x
A

T
|
T

K

-

Y
x
F

M

I

G

G

G
|
G

H
|
H

G

G

T

T

T

T

V
x
M

G

G

G

G

V

V

I

I

V

V

D

E

S

G

G

G

K

Q

F

F

P

D

W

W

A

R

D

A

H

S

G

G

D

-

R

K

F

Y

P

P

M

D

M

F

V

T

E

T

P

P

D

D

P

P

S

Q

Y

Y

H

N

G

G

V

L

V

V

Y

F

T

A

E

-

D

D

M

L

G

G

E

G

A

A

A

A

Y

F

I

T

T

T

R

K

C

V

R

R

V

A

V

E

P

T

L

L

R

R

N

D

M

T

G

G

A

A

A

T

L

I

S

S

P

P

F

F

N

N

A

S

F

F

Q

L

L

L

L

L

Q
|
Q

G

G

I

L

E

E

T
x
S

L

L

P

S

V

L

R

R

M

V

D

E

R

R

H

H

C

V

D

T

N

N

A

T

Q

R

R

K

V

I

A

V

E

A

Y

F

L

L

Q

K

A

A

H

H

P

P

R

K

V

V

S

A

W

W

V

I

K

N

F

Y

A

P

G

E

L

L

E

E

D

D

S

S

P

P

Y

Y

K

H

P

D

L

L

A

A

D

T

R

K

Y

Y

M

F

G

P

G

N

R

G

A

V

A

G

S

S

I

I

L

F

S

T

F

L

G

G

V

L

Q

T

G

G

G

G

F

E

E

A

A

A

G

G

A

K

R

A

F

L

I

I

D

E

A

H

L

L

Q

Q

L

L

I

F

T

S

R

L

L

L

V

A

N

N

I
x
V

G
x
A

D

D

A

A

K

K

S

S

L

L

A

I

C

I

H

H

P

P

A

A

S

S

T

T

T

T

H

H

R

A

Q

Q

L

L

N

N

D

E

E

Q

E

E

L

L

E

L

S

A

A

A

G

G

V

V

S

T

R

P

D

D

L

L

V

I

R

R

L

I

S

S

I

V

G

G

L

V

E

E

H

N

V

A

D

D

D

D

I

L

L

I

A

A

D

D

I

L

D

D

Q

Q

A

A

L

L

binding (2S)-2-amino-6-[[3-hydroxy-2-methyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]hexanoic acid: G87 (= G82), S88 (≠ M83), Y112 (= Y107), E155 (= E150), D184 (= D179), T186 (= T181), S206 (= S201), A207 (≠ L202), T208 (= T203), F209 (≠ Y205), G212 (= G208), M217 (≠ V213), V369 (≠ I367), A370 (≠ G368)
binding proline: H213 (= H209), Q284 (= Q282), S288 (≠ T286)

4hf8A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with glycine (see paper)
40% identity, 99% coverage: 3:421/422 of query aligns to 8:393/396 of 4hf8A

query
sites
4hf8A

L

F

E

N

T

T

Q

Q

A

I

V

V

H

H

A

A

G

G

Y

Q

S

Q

P

P

D

D

P

P

T

S

T

T

R

G

A

A

V

L

A

S

V

T

P

P

I

I

Y

F

Q

Q

T

T

T

S

S

T

Y

F

A

V

F

F

D

D

D

S

T

A

Q

E

H

Q

G

G

A

A

D

A

L

R

F

F

D

A

L

L

K

E

V

E

E

S

G

G

N

Y

I

I

Y

Y

T

T

R
|
R

I

L

M

G

N

N

P

P

T

T

N

T

A

D

V

A

L

L

E

E

Q

K

R

K

V

L

A

A

A

V

M

L

E

E

G

R

G

G

I

E

G

A

G

G

L

L

A

A

V

T

A

A

S

S

G
|
G

M
x
I

A

S

A

A

I

I

T

T

Y

T

A

T

I

L

Q

L

A

T

I

L

T

C

R

Q

A

Q

G

G

D

D

N

H

I

I

V

V

S

S

T

A

S

S

R

A

L

I

Y
|
Y

G

G

G

C

T

T

Y

H

N

A

L

F

F

L

A

S

H

H

S

S

L

M

P

P

I

K

H

F

G

G

I

I

E

N

V

V

R

R

F

F

A

V

A

D

Q

A

D

A

D

K

Y

P

E

E

R

E

L

I

E

R

A

A

Q

A

I

M

D

R

E

P

R

E

T

T

K

K

A

V

L

V

F

Y

C

I

E
|
E

S

T

V

P

G

A

N
|
N

P

P

S

T

G

L

E

S

V

L

V

V

D

D

V

I

E

E

R

T

L

V

A

A

E

G

I

I

A

A

H

H

R

Q

H

Q

G

G

I

A

P

L

L

L

M

V

V

V

D
|
D

N

N

T

T

V

F

P

M

T

S

P

P

Y

Y

L

C

W

Q

R

Q

P

P

I

L

D

Q

N

L

G

G

A

A

D

D

I

I

V

V

I

V

H

H

S
|
S

L

V

T

T

K
|
K

Y

Y

M

I

G

N

G

G

H

H

G

G

T

D

T

V

V

I

G

G

G

G

V

I

I

I

V

V

D

-

S

-

G

-

K

-

F

-

P

-

W

-

A

-

D

-

H

-

G

-

D

-

R

-

F

-

P

-

M

-

M

-

V

-

E

-

P

-

D

-

P

-

S

-

Y

-

H

-

G

-

V

-

V

-

Y

-

T

-

E

-

D

-

M

-

G

G

E

K

A

Q

A

E

Y

F

I

I

T

D

R

Q

C

A

R

R

V

F

V

V

P

G

L

L

R

K

N

D

M

I

-

T

G

G

A

G

A

C

L

M

S

S

P

P

F

F

N

N

A

A

F

W

Q

L

L

T

L

L

Q

R

G

G

I

V

E

K

T

T

L

L

P

G

V

I

R

R

M

M

D

E

R

R

H

H

C

C

D

E

N

N

A

A

Q

L

R

K

V

I

A

A

E

R

Y

F

L

L

Q

E

A

G

H

H

P

P

R

S

V

I

S

T

W

R

V

V

K

Y

F

Y

A

P

G

G

L

L

E

S

D

S

S

H

P

P

Y

Q

K

Y

P

E

L

L

A

G

D

Q

R

R

Y

Q

M

M

G

-

G

S

R

L

A

P

A

G

S

G

I

I

L

I

S

S

F

F

G

E

V

I

Q

A

G

G

G

G

F

L

E

E

A

A

G

G

A

R

R

R

F

M

I

I

D

N

A

S

L

V

Q

E

L

L

I

C

T

L

R

L

L

A

V

V

N
x
S

I

L

G

G

D

D

A

T

K

E

S

T

L

L

A

I

C

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

P

L

V

N

A

D

P

E

E

E

E

L

R

E

L

S

K

A

A

G

G

V

I

S

T

R

D

D

G

L

L

V

I

R
|
R

L

L

S

S

I

V

G

G

L

L

E

E

H

D

V

P

D

E

D

D

I

I

L

I

A

N

D

D

I

L

D

E

Q

H

A

A

L

I

Q

R

active site: R59 (= R54), Y112 (= Y107), D184 (= D179), K209 (= K204)
binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: G87 (= G82), I88 (≠ M83), Y112 (= Y107), E155 (= E150), N159 (= N154), D184 (= D179), S206 (= S201), K209 (= K204), S338 (≠ N366), R373 (= R401)

4omaA The crystal structure of methionine gamma-lyase from citrobacter freundii in complex with l-cycloserine pyridoxal-5'-phosphate (see paper)
40% identity, 99% coverage: 3:421/422 of query aligns to 8:393/396 of 4omaA

query
sites
4omaA

L

F

E

N

T

T

Q

Q

A

I

V

V

H

H

A

A

G

G

Y

Q

S

Q

P

P

D

D

P

P

T

S

T

T

R

G

A

A

V

L

A

S

V

T

P

P

I

I

Y

F

Q

Q

T

T

T

S

S

T

Y

F

A

V

F

F

D

D

D

S

T

A

Q

E

H

Q

G

G

A

A

D

A

L

R

F

F

D

A

L

L

K

E

V

E

E

S

G

G

N

Y

I

I

Y

Y

T

T

R
|
R

I

L

M

G

N

N

P

P

T

T

N

T

A

D

V

A

L

L

E

E

Q

K

R

K

V

L

A

A

A

V

M

L

E

E

G

R

G

G

I

E

G

A

G

G

L

L

A

A

V

T

A

A

S

S

G
|
G

M
x
I

A

S

A

A

I

I

T

T

Y

T

A

T

I

L

Q

L

A

T

I

L

T

C

R

Q

A

Q

G

G

D

D

N

H

I

I

V

V

S

S

T

A

S

S

R

A

L

I

Y
|
Y

G

G

G

C

T

T

Y

H

N

A

L

F

F

L

A

S

H

H

S

S

L

M

P

P

I

K

H

F

G

G

I

I

E

N

V

V

R

S

F

F

A

V

A

D

Q

A

D

A

D

K

Y

P

E

E

R

E

L

I

E

R

A

A

Q

A

I

M

D

R

E

P

R

E

T

T

K

K

A

V

L

V

F

Y

C

I

E

E

S

T

V

P

G

A

N

N

P

P

S

T

G

L

E

S

V

L

V

V

D

D

V

I

E

E

R

T

L

V

A

A

E

G

I

I

A

A

H

H

R

Q

H

Q

G

G

I

A

P

L

L

L

M

V

V

V

D
|
D

N

N

T

T

V

F

P

M

T

S

P

P

Y

Y

L

C

W

Q

R

Q

P

P

I

L

D

Q

N

L

G

G

A

A

D

D

I

I

V

V

I

V

H

H

S
|
S

L

V

T
|
T

K
|
K

Y

Y

M

I

G

N

G

G

H

H

G

G

T

D

T

V

V

I

G

G

G

G

V

I

I

I

V

V

D

-

S

-

G

-

K

-

F

-

P

-

W

-

A

-

D

-

H

-

G

-

D

-

R

-

F

-

P

-

M

-

M

-

V

-

E

-

P

-

D

-

P

-

S

-

Y

-

H

-

G

-

V

-

V

-

Y

-

T

-

E

-

D

-

M

-

G

G

E

K

A

Q

A

E

Y

F

I

I

T

D

R

Q

C

A

R

R

V

F

V

V

P

G

L

L

R

K

N

D

M

I

-

T

G

G

A

G

A

C

L

M

S

S

P

P

F

F

N

N

A

A

F

W

Q

L

L

T

L

L

Q

R

G

G

I

V

E

K

T

T

L

L

P

G

V

I

R

R

M

M

D

E

R

R

H

H

C

C

D

E

N

N

A

A

Q

L

R

K

V

I

A

A

E

R

Y

F

L

L

Q

E

A

G

H

H

P

P

R

S

V

I

S

T

W

R

V

V

K

Y

F

Y

A

P

G

G

L

L

E

S

D

S

S

H

P

P

Y

Q

K

Y

P

E

L

L

A

G

D

Q

R

R

Y

Q

M

M

G

-

G

S

R

L

A

P

A

G

S

G

I

I

L

I

S

S

F

F

G

E

V

I

Q

A

G

G

G

G

F

L

E

E

A

A

G

G

A

R

R

R

F

M

I

I

D

N

A

S

L

V

Q

E

L

L

I

C

T

L

R

L

L

A

V
|
V

N
x
S

I

L

G

G

D

D

A

T

K

E

S

T

L

L

A

I

C

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

P

L

V

N

A

D

P

E

E

E

E

L

R

E

L

S

K

A

A

G

G

V

I

S

T

R

D

D

G

L

L

V

I

R
|
R

L

L

S

S

I

V

G

G

L

L

E

E

H

D

V

P

D

E

D

D

I

I

L

I

A

N

D

D

I

L

D

E

Q

H

A

A

L

I

Q

R

active site: R59 (= R54), Y112 (= Y107), D184 (= D179), K209 (= K204)
binding [5-hydroxy-6-methyl-4-({[(4E)-3-oxo-1,2-oxazolidin-4-ylidene]amino}methyl)pyridin-3-yl]methyl dihydrogen phosphate: G87 (= G82), I88 (≠ M83), Y112 (= Y107), D184 (= D179), S206 (= S201), T208 (= T203), K209 (= K204), V337 (= V365), S338 (≠ N366), R373 (= R401)

3jwbA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with norleucine (see paper)
40% identity, 99% coverage: 3:421/422 of query aligns to 8:393/396 of 3jwbA

query
sites
3jwbA

L

F

E

N

T

T

Q

Q

A

I

V

V

H

H

A

A

G

G

Y

Q

S

Q

P

P

D

D

P

P

T

S

T

T

R

G

A

A

V

L

A

S

V

T

P

P

I

I

Y

F

Q

Q

T

T

T

S

S

T

Y

F

A

V

F

F

D

D

D

S

T

A

Q

E

H

Q

G

G

A

A

D

A

L

R

F

F

D

A

L

L

K

E

V

E

E

S

G

G

N

Y

I

I

Y

Y

T

T

R
|
R

I

L

M

G

N

N

P

P

T

T

N

T

A

D

V

A

L

L

E

E

Q

K

R

K

V

L

A

A

A

V

M

L

E

E

G

R

G

G

I

E

G

A

G

G

L

L

A

A

V

T

A

A

S

S

G

G

M

I

A

S

A

A

I

I

T

T

Y

T

A

T

I

L

Q

L

A

T

I

L

T

C

R

Q

A

Q

G

G

D

D

N

H

I

I

V

V

S

S

T

A

S

S

R

A

L

I

Y
|
Y

G

G

G

C

T

T

Y

H

N

A

L

F

F

L

A

S

H

H

S

S

L

M

P

P

I

K

H

F

G

G

I

I

E

N

V

V

R

S

F

F

A

V

A

D

Q

A

D

A

D

K

Y

P

E

E

R

E

L

I

E

R

A

A

Q

A

I

M

D

R

E

P

R

E

T

T

K

K

A

V

L

V

F

Y

C

I

E

E

S

T

V

P

G

A

N

N

P

P

S

T

G

L

E

S

V

L

V

V

D

D

V

I

E

E

R

T

L

V

A

A

E

G

I

I

A

A

H

H

R

Q

H

Q

G

G

I

A

P

L

L

L

M

V

V

V

D
|
D

N

N

T

T

V

F

P

M

T

S

P

P

Y

Y

L

C

W

Q

R

Q

P

P

I

L

D

Q

N

L

G

G

A

A

D

D

I

I

V

V

I

V

H

H

S

S

L

V

T

T

K
|
K

Y

Y

M

I

G

N

G

G

H

H

G

G

T

D

T

V

V

I

G

G

G

G

V

I

I

I

V

V

D

-

S

-

G

-

K

-

F

-

P

-

W

-

A

-

D

-

H

-

G

-

D

-

R

-

F

-

P

-

M

-

M

-

V

-

E

-

P

-

D

-

P

-

S

-

Y

-

H

-

G

-

V

-

V

-

Y

-

T

-

E

-

D

-

M

-

G

G

E

K

A

Q

A

E

Y

F

I

I

T

D

R

Q

C

A

R

R

V

F

V

V

P

G

L

L

R

K

N

D

M

I

-

T

G

G

A

G

A

C

L

M

S

S

P

P

F

F

N

N

A

A

F

W

Q

L

L

T

L

L

Q

R

G

G

I

V

E

K

T

T

L

L

P

G

V

I

R

R

M

M

D

E

R

R

H

H

C

C

D

E

N

N

A

A

Q

L

R

K

V

I

A

A

E

R

Y

F

L

L

Q

E

A

G

H

H

P

P

R

S

V

I

S

T

W

R

V

V

K

Y

F

Y

A

P

G

G

L

L

E

S

D

S

S

H

P

P

Y

Q

K

Y

P

E

L

L

A

G

D

Q

R

R

Y

Q

M

M

G

-

G

S

R

L

A

P

A

G

S

G

I

I

L

I

S

S

F

F

G

E

V

I

Q

A

G

G

G

G

F

L

E

E

A

A

G

G

A

R

R

R

F

M

I

I

D

N

A

S

L

V

Q

E

L

L

I

C

T

L

R

L

L

A

V

V

N
x
S

I

L

G

G

D

D

A

T

K

E

S

T

L

L

A

I

C

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

P

L

V

N

A

D

P

E

E

E

E

L

R

E

L

S

K

A

A

G

G

V

I

S

T

R

D

D

G

L

L

V

I

R
|
R

L

L

S

S

I

V

G

G

L

L

E

E

H

D

V

P

D

E

D

D

I

I

L

I

A

N

D

D

I

L

D

E

Q

H

A

A

L

I

Q

R

active site: R59 (= R54), Y112 (= Y107), D184 (= D179), K209 (= K204)
binding norleucine: Y112 (= Y107), K209 (= K204), S338 (≠ N366), R373 (= R401)

3jwaA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with methionine phosphinate (see paper)
40% identity, 99% coverage: 3:421/422 of query aligns to 8:393/396 of 3jwaA

query
sites
3jwaA

L

F

E

N

T

T

Q

Q

A

I

V

V

H

H

A

A

G

G

Y

Q

S

Q

P

P

D

D

P

P

T

S

T

T

R

G

A

A

V

L

A

S

V

T

P

P

I

I

Y

F

Q

Q

T

T

T

S

S

T

Y

F

A

V

F

F

D

D

D

S

T

A

Q

E

H

Q

G

G

A

A

D

A

L

R

F

F

D

A

L

L

K

E

V

E

E

S

G

G

N

Y

I

I

Y

Y

T

T

R
|
R

I

L

M

G

N

N

P

P

T

T

N

T

A

D

V

A

L

L

E

E

Q

K

R

K

V

L

A

A

A

V

M

L

E

E

G

R

G

G

I

E

G

A

G

G

L

L

A

A

V

T

A

A

S

S

G

G

M

I

A

S

A

A

I

I

T

T

Y

T

A

T

I

L

Q

L

A

T

I

L

T

C

R

Q

A

Q

G

G

D

D

N

H

I

I

V

V

S

S

T

A

S

S

R

A

L

I

Y
|
Y

G

G

G

C

T

T

Y

H

N

A

L

F

F

L

A

S

H

H

S

S

L

M

P

P

I

K

H

F

G

G

I

I

E

N

V

V

R

S

F

F

A

V

A

D

Q

A

D

A

D

K

Y

P

E

E

R

E

L

I

E

R

A

A

Q

A

I

M

D

R

E

P

R

E

T

T

K

K

A

V

L

V

F

Y

C

I

E

E

S

T

V

P

G

A

N

N

P

P

S

T

G

L

E

S

V

L

V

V

D

D

V

I

E

E

R

T

L

V

A

A

E

G

I

I

A

A

H

H

R

Q

H

Q

G

G

I

A

P

L

L

L

M

V

V

V

D
|
D

N

N

T

T

V

F

P

M

T

S

P

P

Y

Y

L

C

W

Q

R

Q

P

P

I

L

D

Q

N

L

G

G

A

A

D

D

I

I

V

V

I

V

H

H

S

S

L

V

T

T

K
|
K

Y

Y

M

I

G

N

G

G

H

H

G

G

T

D

T

V

V

I

G

G

G

G

V

I

I

I

V

V

D

-

S

-

G

-

K

-

F

-

P

-

W

-

A

-

D

-

H

-

G

-

D

-

R

-

F

-

P

-

M

-

M

-

V

-

E

-

P

-

D

-

P

-

S

-

Y

-

H

-

G

-

V

-

V

-

Y

-

T

-

E

-

D

-

M

-

G

G

E

K

A

Q

A

E

Y

F

I

I

T

D

R

Q

C

A

R

R

V

F

V

V

P

G

L

L

R

K

N

D

M

I

-

T

G

G

A

G

A

C

L

M

S

S

P

P

F

F

N

N

A

A

F

W

Q

L

L

T

L

L

Q

R

G

G

I

V

E

K

T

T

L

L

P

G

V

I

R

R

M

M

D

E

R

R

H

H

C

C

D

E

N

N

A

A

Q

L

R

K

V

I

A

A

E

R

Y

F

L

L

Q

E

A

G

H

H

P

P

R

S

V

I

S

T

W

R

V

V

K

Y

F

Y

A

P

G

G

L

L

E

S

D

S

S

H

P

P

Y

Q

K

Y

P

E

L

L

A

G

D

Q

R

R

Y

Q

M

M

G

-

G

S

R

L

A

P

A

G

S

G

I

I

L

I

S

S

F

F

G

E

V

I

Q

A

G

G

G

G

F

L

E

E

A

A

G

G

A

R

R

R

F

M

I

I

D

N

A

S

L

V

Q

E

L

L

I

C

T

L

R

L

L

A

V
|
V

N
x
S

I

L

G

G

D

D

A

T

K

E

S

T

L

L

A

I

C

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

P

L

V

N

A

D

P

E

E

E

E

L

R

E

L

S

K

A

A

G

G

V

I

S

T

R

D

D

G

L

L

V

I

R
|
R

L

L

S

S

I

V

G

G

L

L

E

E

H

D

V

P

D

E

D

D

I

I

L

I

A

N

D

D

I

L

D

E

Q

H

A

A

L

I

Q

R

active site: R59 (= R54), Y112 (= Y107), D184 (= D179), K209 (= K204)
binding (1-amino-3-methylsulfanyl-propyl)-phosphinic acid: Y112 (= Y107), K209 (= K204), V337 (= V365), S338 (≠ N366), R373 (= R401)

3jw9A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with s-ethyl-cysteine (see paper)
40% identity, 99% coverage: 3:421/422 of query aligns to 8:393/396 of 3jw9A

query
sites
3jw9A

L

F

E

N

T

T

Q

Q

A

I

V

V

H

H

A

A

G

G

Y

Q

S

Q

P

P

D

D

P

P

T

S

T

T

R

G

A

A

V

L

A

S

V

T

P

P

I

I

Y

F

Q

Q

T

T

T

S

S

T

Y

F

A

V

F

F

D

D

D

S

T

A

Q

E

H

Q

G

G

A

A

D

A

L

R

F

F

D

A

L

L

K

E

V

E

E

S

G

G

N

Y

I

I

Y

Y

T

T

R
|
R

I

L

M

G

N

N

P

P

T

T

N

T

A

D

V

A

L

L

E

E

Q

K

R

K

V

L

A

A

A

V

M

L

E

E

G

R

G

G

I

E

G

A

G

G

L

L

A

A

V

T

A

A

S

S

G

G

M

I

A

S

A

A

I

I

T

T

Y

T

A

T

I

L

Q

L

A

T

I

L

T

C

R

Q

A

Q

G

G

D

D

N

H

I

I

V

V

S

S

T

A

S

S

R

A

L

I

Y
|
Y

G

G

G

C

T

T

Y

H

N

A

L

F

F

L

A

S

H

H

S

S

L

M

P

P

I

K

H

F

G

G

I

I

E

N

V

V

R

S

F

F

A

V

A

D

Q

A

D

A

D

K

Y

P

E

E

R

E

L

I

E

R

A

A

Q

A

I

M

D

R

E

P

R

E

T

T

K

K

A

V

L

V

F

Y

C

I

E

E

S

T

V

P

G

A

N

N

P

P

S

T

G

L

E

S

V

L

V

V

D

D

V

I

E

E

R

T

L

V

A

A

E

G

I

I

A

A

H

H

R

Q

H

Q

G

G

I

A

P

L

L

L

M

V

V

V

D
|
D

N

N

T

T

V

F

P

M

T

S

P

P

Y

Y

L

C

W

Q

R

Q

P

P

I

L

D

Q

N

L

G

G

A

A

D

D

I

I

V

V

I

V

H

H

S

S

L

V

T

T

K
|
K

Y

Y

M

I

G

N

G

G

H

H

G

G

T

D

T

V

V

I

G

G

G

G

V

I

I

I

V

V

D

-

S

-

G

-

K

-

F

-

P

-

W

-

A

-

D

-

H

-

G

-

D

-

R

-

F

-

P

-

M

-

M

-

V

-

E

-

P

-

D

-

P

-

S

-

Y

-

H

-

G

-

V

-

V

-

Y

-

T

-

E

-

D

-

M

-

G

G

E

K

A

Q

A

E

Y

F

I

I

T

D

R

Q

C

A

R

R

V

F

V

V

P

G

L

L

R

K

N

D

M

I

-

T

G

G

A

G

A

C

L

M

S

S

P

P

F

F

N

N

A

A

F

W

Q

L

L

T

L

L

Q

R

G

G

I

V

E

K

T

T

L

L

P

G

V

I

R

R

M

M

D

E

R

R

H

H

C

C

D

E

N

N

A

A

Q

L

R

K

V

I

A

A

E

R

Y

F

L

L

Q

E

A

G

H

H

P

P

R

S

V

I

S

T

W

R

V

V

K

Y

F

Y

A

P

G

G

L

L

E

S

D

S

S

H

P

P

Y

Q

K

Y

P

E

L

L

A

G

D

Q

R

R

Y

Q

M

M

G

-

G

S

R

L

A

P

A

G

S

G

I

I

L

I

S

S

F

F

G

E

V

I

Q

A

G

G

G

G

F

L

E

E

A

A

G

G

A

R

R

R

F

M

I

I

D

N

A

S

L

V

Q

E

L

L

I

C

T

L

R

L

L

A

V
|
V

N
x
S

I

L

G

G

D

D

A

T

K

E

S

T

L

L

A

I

C

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

P

L

V

N

A

D

P

E

E

E

E

L

R

E

L

S

K

A

A

G

G

V

I

S

T

R

D

D

G

L

L

V

I

R

R

L

L

S

S

I

V

G

G

L

L

E

E

H

D

V

P

D

E

D

D

I

I

L

I

A

N

D

D

I

L

D

E

Q

H

A

A

L

I

Q

R

active site: R59 (= R54), Y112 (= Y107), D184 (= D179), K209 (= K204)
binding S-ethyl-L-cysteine: Y112 (= Y107), K209 (= K204), V337 (= V365), S338 (≠ N366)

5m3zA Crystal structure of citrobacter freundii methionine gamma-lyase with c115h replacement in the complex with l-norleucine (see paper)
40% identity, 99% coverage: 3:421/422 of query aligns to 7:392/395 of 5m3zA

query
sites
5m3zA

L

F

E

N

T

T

Q

Q

A

I

V

V

H

H

A

A

G

G

Y

Q

S

Q

P

P

D

D

P

P

T

S

T

T

R

G

A

A

V

L

A

S

V

T

P

P

I

I

Y

F

Q

Q

T

T

T

S

S

T

Y

F

A

V

F

F

D

D

D

S

T

A

Q

E

H

Q

G

G

A

A

D

A

L

R

F

F

D

A

L

L

K

E

V

E

E

S

G

G

N

Y

I

I

Y

Y

T

T

R
|
R

I

L

M

G

N

N

P

P

T

T

N

T

A

D

V

A

L

L

E

E

Q

K

R

K

V

L

A

A

A

V

M

L

E

E

G

R

G

G

I

E

G

A

G

G

L

L

A

A

V

T

A

A

S

S

G
|
G

M
x
I

A

S

A

A

I

I

T

T

Y

T

A

T

I

L

Q

L

A

T

I

L

T

C

R

Q

A

Q

G

G

D

D

N

H

I

I

V

V

S

S

T

A

S

S

R

A

L

I

Y
|
Y

G

G

G
x
H

T

T

Y

H

N

A

L

F

F

L

A

S

H

H

S

S

L

M

P

P

I

K

H

F

G

G

I

I

E

N

V

V

R

S

F

F

A

V

A

D

Q

A

D

A

D

K

Y

P

E

E

R

E

L

I

E

R

A

A

Q

A

I

M

D

R

E

P

R

E

T

T

K

K

A

V

L

V

F

Y

C

I

E
|
E

S

T

V

P

G

A

N

N

P

P

S

T

G

L

E

S

V

L

V

V

D

D

V

I

E

E

R

T

L

V

A

A

E

G

I

I

A

A

H

H

R

Q

H

Q

G

G

I

A

P

L

L

L

M

V

V

V

D
|
D

N

N

T
|
T

V

F

P

M

T

S

P

P

Y

Y

L

C

W

Q

R

Q

P

P

I

L

D

Q

N

L

G

G

A

A

D

D

I

I

V

V

I

V

H

H

S
|
S

L

V

T
|
T

K
|
K

Y

Y

M

I

G

N

G

G

H

H

G

G

T

D

T

V

V

I

G

G

G

G

V

I

I

I

V

V

D

-

S

-

G

-

K

-

F

-

P

-

W

-

A

-

D

-

H

-

G

-

D

-

R

-

F

-

P

-

M

-

M

-

V

-

E

-

P

-

D

-

P

-

S

-

Y

-

H

-

G

-

V

-

V

-

Y

-

T

-

E

-

D

-

M

-

G

G

E

K

A

Q

A

E

Y

F

I

I

T

D

R

Q

C

A

R

R

V

F

V

V

P

G

L

L

R

K

N

D

M

I

-

T

G

G

A

G

A

C

L

M

S

S

P

P

F

F

N

N

A

A

F

W

Q

L

L

T

L

L

Q

R

G

G

I

V

E

K

T

T

L

L

P

G

V

I

R

R

M

M

D

E

R

R

H

H

C

C

D

E

N

N

A

A

Q

L

R

K

V

I

A

A

E

R

Y

F

L

L

Q

E

A

G

H

H

P

P

R

S

V

I

S

T

W

R

V

V

K

Y

F

Y

A

P

G

G

L

L

E

S

D

S

S

H

P

P

Y

Q

K

Y

P

E

L

L

A

G

D

Q

R

R

Y

Q

M

M

G

-

G

S

R

L

A

P

A

G

S

G

I

I

L

I

S

S

F

F

G

E

V

I

Q

A

G

G

G

G

F

L

E

E

A

A

G

G

A

R

R

R

F

M

I

I

D

N

A

S

L

V

Q

E

L

L

I

C

T

L

R

L

L

A

V
|
V

N
x
S

I

L

G

G

D

D

A

T

K

E

S

T

L

L

A

I

C

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

P

L

V

N

A

D

P

E

E

E

E

L

R

E

L

S

K

A

A

G

G

V

I

S

T

R

D

D

G

L

L

V

I

R
|
R

L

L

S

S

I

V

G

G

L

L

E

E

H

D

V

P

D

E

D

D

I

I

L

I

A

N

D

D

I

L

D

E

Q

H

A

A

L

I

Q

R

active site: R58 (= R54), Y111 (= Y107), D183 (= D179), K208 (= K204)
binding norleucine: Y111 (= Y107), H113 (≠ G109), K208 (= K204), V336 (= V365), S337 (≠ N366)
binding pyridoxal-5'-phosphate: G86 (= G82), I87 (≠ M83), Y111 (= Y107), E154 (= E150), D183 (= D179), T185 (= T181), S205 (= S201), T207 (= T203), K208 (= K204)
binding 2-[o-phosphonopyridoxyl]-amino-hexanoic acid: G86 (= G82), I87 (≠ M83), Y111 (= Y107), D183 (= D179), S205 (= S201), T207 (= T203), K208 (= K204), V336 (= V365), S337 (≠ N366), R372 (= R401)

6egrA Crystal structure of citrobacter freundii methionine gamma-lyase with v358y replacement (see paper)
40% identity, 99% coverage: 3:421/422 of query aligns to 8:393/396 of 6egrA

query
sites
6egrA

L

F

E

N

T

T

Q

Q

A

I

V

V

H

H

A

A

G

G

Y

Q

S

Q

P

P

D

D

P

P

T

S

T

T

R

G

A

A

V

L

A

S

V

T

P

P

I

I

Y

F

Q

Q

T

T

T

S

S

T

Y

F

A

V

F

F

D

D

D

S

T

A

Q

E

H

Q

G

G

A

A

D

A

L

R

F

F

D

A

L

L

K

E

V

E

E

S

G

G

N

Y

I

I

Y

Y

T

T

R

R

I

L

M

G

N

N

P

P

T

T

N

T

A

D

V

A

L

L

E

E

Q

K

R

K

V

L

A

A

A

V

M

L

E

E

G

R

G

G

I

E

G

A

G

G

L

L

A

A

V

T

A

A

S

S

G
|
G

M
x
I

A

S

A

A

I

I

T

T

Y

T

A

T

I

L

Q

L

A

T

I

L

T

C

R

Q

A

Q

G

G

D

D

N

H

I

I

V

V

S

S

T

A

S

S

R

A

L

I

Y
|
Y

G

G

G

C

T

T

Y

H

N

A

L

F

F

L

A

S

H

H

S

S

L

M

P

P

I

K

H

F

G

G

I

I

E

N

V

V

R

S

F

F

A

V

A

D

Q

A

D

A

D

K

Y

P

E

E

R

E

L

I

E

R

A

A

Q

A

I

M

D

R

E

P

R

E

T

T

K

K

A

V

L

V

F

Y

C

I

E
|
E

S

T

V

P

G

A

N

N

P

P

S

T

G

L

E

S

V

L

V

V

D

D

V

I

E

E

R

T

L

V

A

A

E

G

I

I

A

A

H

H

R

Q

H

Q

G

G

I

A

P

L

L

L

M

V

V

V

D
|
D

N

N

T
|
T

V
x
F

P

M

T

S

P

P

Y

Y

L

C

W

Q

R

Q

P

P

I

L

D

Q

N

L

G

G

A

A

D

D

I

I

V

V

I

V

H

H

S
|
S

L

V

T
|
T

K
|
K

Y

Y

M

I

G

N

G

G

H

H

G

G

T

D

T

V

V

I

G

G

G

G

V

I

I

I

V

V

D

-

S

-

G

-

K

-

F

-

P

-

W

-

A

-

D

-

H

-

G

-

D

-

R

-

F

-

P

-

M

-

M

-

V

-

E

-

P

-

D

-

P

-

S

-

Y

-

H

-

G

-

V

-

V

-

Y

-

T

-

E

-

D

-

M

-

G

G

E

K

A

Q

A

E

Y

F

I

I

T

D

R

Q

C

A

R

R

V

F

V

V

P

G

L

L

R

K

N

D

M

I

-

T

G

G

A

G

A

C

L

M

S

S

P

P

F

F

N

N

A

A

F

W

Q

L

L

T

L

L

Q

R

G

G

I

V

E

K

T

T

L

L

P

G

V

I

R

R

M

M

D

E

R

R

H

H

C

C

D

E

N

N

A

A

Q

L

R

K

V

I

A

A

E

R

Y

F

L

L

Q

E

A

G

H

H

P

P

R

S

V

I

S

T

W

R

V

V

K

Y

F

Y

A

P

G

G

L

L

E

S

D

S

S

H

P

P

Y

Q

K

Y

P

E

L

L

A

G

D

Q

R

R

Y

Q

M

M

G

-

G

S

R

L

A

P

A

G

S

G

I

I

L

I

S

S

F

F

G

E

V

I

Q

A

G

G

G

G

F

L

E

E

A

A

G

G

A

R

R

R

F

M

I

I

D

N

A

S

L

V

Q

E

L

L

I

C

T

L

R

L

L

A

V

V

N

S

I

L

G

G

D

D

A

T

K

E

S

T

L

L

A

I

C

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

P

L

Y

N

A

D

P

E

E

E

E

L

R

E

L

S

K

A

A

G

G

V

I

S

T

R

D

D

G

L

L

V

I

R

R

L

L

S

S

I

V

G

G

L

L

E

E

H

D

V

P

D

E

D

D

I

I

L

I

A

N

D

D

I

L

D

E

Q

H

A

A

L

I

Q

R

binding pyridoxal-5'-phosphate: G87 (= G82), I88 (≠ M83), Y112 (= Y107), E155 (= E150), D184 (= D179), T186 (= T181), F187 (≠ V182), S206 (= S201), T208 (= T203), K209 (= K204)

8ovhA Crystal structure of o-acetyl-l-homoserine sulfhydrolase from saccharomyces cerevisiae in complex with pyridoxal-5'-phosphate (see paper)
45% identity, 99% coverage: 3:421/422 of query aligns to 5:392/400 of 8ovhA

query
sites
8ovhA

L

F

E

D

T

T

Q

V

A

Q

V

L

H

H

A

A

G

G

Y

-

S

-

P

-

D

-

P

-

T

Q

T

E

R

N

A

S

V

R

A

A

V

V

P

P

I

I

Y

Y

Q

A

T

T

T

T

S

S

Y

Y

A

-

F

-

D

-

D

-

T

-

Q

-

H

-

G

-

A

-

D

-

L

-

F

-

D

-

L

-

K

-

V

-

E

-

G

-

N

-

I

-

Y

-

T

-

R

-

I

-

M

-

N

N

P

P

T

T

N

S

A

N

V

V

L

L

E

E

Q

E

R

R

V

I

A

A

A

A

M

L

E

E

G

G

G

G

I

A

G

A

G

A

L

L

A

A

V

V

A

S

S

S

G
|
G

M
x
Q

A

A

A

A

I
x
Q

T

T

Y

L

A

A

I

I

Q

Q

A

G

I

L

T

A

R

H

A

T

G

G

D

D

N

N

I

I

V

V

S

S

T

T

S

S

R

Y

L

L

Y
|
Y

G

G

G

G

T

T

Y

Y

N

N

L

Q

F

F

A

K

H

I

S

S

L

F

P

K

I

R

H

F

G

G

I

I

E

E

V

A

R

R

F

F

A

V

A

E

Q

G

D

D

D

N

Y

P

E

E

R

E

L

F

E

E

A

K

Q

V

I

F

D

D

E

E

R

R

T

T

K

K

A

A

L

V

F

Y

C

L

E

E

S

T

V

I

G

G

N

N

P

P

S

K

G

Y

E

N

V

V

V

P

D

D

V

F

E

E

R

K

L

I

A

V

E

A

I

I

A

A

H

H

R

K

H

H

G

G

I

I

P

P

L

V

M

V

V

V

D
|
D

N

N

T
|
T

V

F

P

G

T

A

P

G

-

G

Y

Y

L

F

W

C

R

Q

P

P

I

I

D

K

N

Y

G

G

A

A

D

D

I

I

V

V

I

T

H

H

S
|
S

L

A

T
|
T

K
|
K

Y

W

M

I

G

G

G

G

H

H

G

G

T

T

T

T

V

I

G

G

G

G

V

I

I

I

V

V

D

D

S

S

G

G

K

K

F

F

P

P

W

W

A

K

D

D

H

Y

G

P

D

E

R

K

F

F

P

P

M

Q

M

F

V

S

E

Q

P

P

D

A

P

E

S

G

Y

Y

H

H

G

G

V

T

V

I

Y

Y

T

N

E

E

D

A

M

Y

G

G

E

N

A

L

A

A

Y

Y

I

I

T

V

R

H

C

V

R

R

V

T

V

E

P

L

L

L

R

R

N

D

M

L

G

G

A

P

A

L

L

M

S

N

P

P

F

F

N

A

A

S

F

F

Q

L

L

L

L

L

Q

Q

G

G

I

V

E

E

T

T

L

L

P

S

V

L

R

R

M

A

D

E

R

R

H

H

C

G

D

E

N

N

A

A

Q

L

R

K

V

L

A

A

E

K

Y

W

L

L

Q

E

A

Q

H

S

P

P

R

Y

V

V

S

S

W

W

V

V

K

S

F

Y

A

P

G

G

L

L

E

A

D

S

S

H

P

S

Y

H

K

H

P

E

L

N

A

A

D

K

R

K

Y

Y

M

L

G

S

G

N

R

G

A

F

A

G

S

G

I

V

L

L

S

S

F

F

G

G

V

V

Q

K

G

D

G

L

F

P

E

N

A

A

-

D

-

K

-

E

-

T

-

D

-

P

-

F

-

K

-

L

-

S

G

G

A

A

R

Q

F

V

I

V

D

D

A

N

L

L

Q

K

L

L

I

A

T

S

R

N

L

L

V

A

N

N

I

V

G

G

D

D

A

A

K

K

S

T

L

L

A

V

C

I

H

A

P

P

A

Y

S

F

T

T

T

T

H

-

R

-

Q

-

L

-

N

-

D

-

E

-

E

-

L

-

E

-

S

-

A

-

G

G

V

V

S

T

R

K

D

D

L

L

V

I

R

R

L

V

S

S

I

V

G

G

L

I

E

E

H

F

V

I

D

D

D

D

I

I

L

I

A

A

D

D

I

F

D

Q

Q

Q

A

S

L

F

Q

E

binding pyridoxal-5'-phosphate: G54 (= G82), Q55 (≠ M83), Q58 (≠ I86), Y79 (= Y107), D151 (= D179), T153 (= T181), S174 (= S201), T176 (= T203), K177 (= K204)

5x30C Crystal structure of pseudomonas putida methionine gamma-lyase c116h mutant with l-homocysteine intermediates. (see paper)
41% identity, 99% coverage: 5:422/422 of query aligns to 7:391/393 of 5x30C

query
sites
5x30C

T

T

Q

R

A

A

V

I

H

H

A

H

G

G

Y

Y

S

D

P

P

D

Q

P

D

T

H

T

G

R

G

A

A

V

L

A

V

V

P

P

P

I

V

Y

Y

Q

Q

T

T

T

A

S

T

Y

F

A

T

F

F

D

P

D

T

T

V

Q

E

H

Y

G

G

A

A

D

A

L

C

F

F

D

A

L

G

K

E

V

Q

E

A

G

G

N

H

I

F

Y
|
Y

T

S

R
|
R

I

I

M

S

N

N

P

P

T

T

N

L

A

N

V

L

L

L

E

E

Q

A

R

R

V

M

A

A

A

S

M

L

E

E

G

G

G

G

I

E

G

A

G

G

L

L

A

A

V

L

A

A

S

S

G

G

M

M

A

G

A

A

I

I

T

T

Y

S

A

T

I

L

Q

W

A

T

I

L

T

L

R

R

A

P

G

G

D

D

N

E

I

V

V

L

S

L

T

G

S

N

R

T

L

L

Y
|
Y

G

G

G

H

T

T

Y

F

N

A

L

F

F

L

A

H

H

H

S

G

L

I

P

G

I

E

H

F

G

G

I

V

E

K

V

L

R

R

F

H

A

V

A

D

Q

M

D

A

D

D

Y

L

E

Q

R

A

L

L

E

E

A

A

Q

A

I

M

D

T

E

P

R

A

T

T

K

R

A

V

L

I

F

Y

C

F

E

E

S

S

V

P

G

A

N

N

P

P

S

N

G

M

E

H

V

M

V

A

D

D

V

I

E

A

R

G

L

V

A

A

E

K

I

I

A

A

H

R

R

K

H

H

G

G

I

A

P

T

L

V

M

V

V

V

D
|
D

N

N

T

T

V

Y

P

C

T

T

P

P

Y

Y

L

L

W

Q

R

R

P

P

I

L

D

E

N

L

G

G

A

A

D

D

I

L

V

V

I

V

H

H

S

S

L

A

T

T

K
|
K

Y

Y

M

L

G

S

G

G

H

H

G

G

T

D

T

I

V

T

G

A

G

G

V

I

I

V

V

V

D

-

S

-

G

-

K

-

F

-

P

-

W

-

A

-

D

-

H

-

G

-

D

-

R

-

F

-

P

-

M

-

M

-

V

-

E

-

P

-

D

-

P

-

S

-

Y

-

H

-

G

-

V

-

V

-

Y

-

T

-

E

-

D

-

M

-

G

G

E

S

A

Q

A

A

Y

L

I

V

T

D

R

R

C

I

R

R

V

L

V

Q

P

G

L

L

R

K

N

D

M

M

-

T

G

G

A

A

A

V

L

L

S

S

P

P

F

H

N

D

A

A

F

A

Q

L

L

L

L

M

Q

R

G

G

I

I

E

K

T

T

L

L

P

N

V

L

R

R

M

M

D

D

R

R

H

H

C

C

D

A

N

N

A

A

Q

Q

R

V

V

L

A

A

E

E

Y

F

L

L

Q

A

A

R

H

Q

P

P

R

Q

V

V

S

E

W

L

V

I

K

H

F

Y

A

P

G

G

L

L

E

A

D

S

S

F

P

P

Y

Q

K

Y

P

T

L

L

A

A

D

R

R

Q

Y

Q

M

M

G

S

G

-

R

Q

A

P

A

G

S

G

I

M

L

I

S

A

F

F

G

E

V

L

Q

K

G

G

G

G

F

I

E

G

A

A

G

G

A

R

R

R

F

F

I

M

D

N

A

A

L

L

Q

Q

L

L

I

F

T

S

R

R

L

A

V

V

N

S

I

L

G

G

D

D

A

A

K

E

S

S

L

L

A

A

C

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

S

L

Y

N

T

D

P

E

E

E

E

L

R

E

A

S

H

A

Y

G

G

V

I

S

S

R

E

D

G

L

L

V

V

R

R

L

L

S

S

I

V

G

G

L

L

E

E

H

D

V

I

D

D

D

D

I

L

L

L

A

A

D

D

I

V

D

Q

Q

Q

A

A

L

L

Q

K

A

A

active site: R56 (= R54), Y109 (= Y107), D181 (= D179), K206 (= K204)
binding (2~{S})-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylamino]-4-sulfanyl-butanoic acid: Y54 (= Y52), R56 (= R54)

5x2xA Crystal structure of pseudomonas putida methionine gamma-lyase wild type with l-homocysteine intermediates (see paper)
41% identity, 99% coverage: 5:422/422 of query aligns to 6:390/392 of 5x2xA

query
sites
5x2xA

T

T

Q

R

A

A

V

I

H

H

A

H

G

G

Y

Y

S

D

P

P

D

Q

P

D

T

H

T

G

R

G

A

A

V

L

A

V

V

P

P

P

I

V

Y

Y

Q

Q

T

T

T

A

S

T

Y

F

A

T

F

F

D

P

D

T

T

V

Q

E

H

Y

G

G

A

A

D

A

L

C

F

F

D

A

L

G

K

E

V

Q

E

A

G

G

N

H

I

F

Y
|
Y

T

S

R
|
R

I

I

M

S

N

N

P

P

T

T

N

L

A

N

V

L

L

L

E

E

Q

A

R

R

V

M

A

A

A

S

M

L

E

E

G

G

G

G

I

E

G

A

G

G

L

L

A

A

V

L

A

A

S

S

G
|
G

M
|
M

A

G

A

A

I

I

T

T

Y

S

A

T

I

L

Q

W

A

T

I

L

T

L

R

R

A

P

G

G

D

D

N

E

I

V

V

L

S

L

T

G

S

N

R

T

L

L

Y
|
Y

G

G

G

C

T

T

Y

F

N

A

L

F

F

L

A

H

H

H

S

G

L

I

P

G

I

E

H

F

G

G

I

V

E

K

V

L

R

R

F

H

A

V

A

D

Q

M

D

A

D

D

Y

L

E

Q

R

A

L

L

E

E

A

A

Q

A

I

M

D

T

E

P

R

A

T

T

K

R

A

V

L

I

F

Y

C

F

E

E

S

S

V

P

G

A

N
|
N

P

P

S

N

G

M

E

H

V

M

V

A

D

D

V

I

E

A

R

G

L

V

A

A

E

K

I

I

A

A

H

R

R

K

H

H

G

G

I

A

P

T

L

V

M

V

V

V

D
|
D

N

N

T

T

V

Y

P

C

T

T

P

P

Y

Y

L

L

W

Q

R

R

P

P

I

L

D

E

N

L

G

G

A

A

D

D

I

L

V

V

I

V

H

H

S
|
S

L

A

T
|
T

K
|
K

Y

Y

M

L

G

S

G

G

H

H

G

G

T

D

T

I

V

T

G

A

G

G

V

I

I

V

V

V

D

-

S

-

G

-

K

-

F

-

P

-

W

-

A

-

D

-

H

-

G

-

D

-

R

-

F

-

P

-

M

-

M

-

V

-

E

-

P

-

D

-

P

-

S

-

Y

-

H

-

G

-

V

-

V

-

Y

-

T

-

E

-

D

-

M

-

G

G

E

S

A

Q

A

A

Y

L

I

V

T

D

R

R

C

I

R

R

V

L

V

Q

P

G

L

L

R

K

N

D

M

M

-

T

G

G

A

A

A

V

L

L

S

S

P

P

F

H

N

D

A

A

F

A

Q

L

L

L

L

M

Q

R

G

G

I

I

E

K

T

T

L

L

P

N

V

L

R

R

M

M

D

D

R

R

H

H

C

C

D

A

N

N

A

A

Q

Q

R

V

V

L

A

A

E

E

Y

F

L

L

Q

A

A

R

H

Q

P

P

R

Q

V

V

S

E

W

L

V

I

K

H

F

Y

A

P

G

G

L

L

E

A

D

S

S

F

P

P

Y

Q

K

Y

P

T

L

L

A

A

D

R

R

Q

Y

Q

M

M

G

S

G

-

R

Q

A

P

A

G

S

G

I

M

L

I

S

A

F

F

G

E

V

L

Q

K

G

G

G

G

F

I

E

G

A

A

G

G

A

R

R

R

F

F

I

M

D

N

A

A

L

L

Q

Q

L

L

I

F

T

S

R

R

L

A

V
|
V

N
x
S

I

L

G

G

D

D

A

A

K

E

S

S

L

L

A

A

C

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

S

L

Y

N

T

D

P

E

E

E

E

L

R

E

A

S

H

A

Y

G

G

V

I

S

S

R

E

D

G

L

L

V

V

R
|
R

L

L

S

S

I

V

G

G

L

L

E

E

H

D

V

I

D

D

D

D

I

L

L

L

A

A

D

D

I

V

D

Q

Q

Q

A

A

L

L

Q

K

A

A

active site: R55 (= R54), Y108 (= Y107), D180 (= D179), K205 (= K204)
binding (2E)-2-{[(1E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}but-2-enoic acid: Y53 (= Y52), R55 (= R54), G83 (= G82), M84 (= M83), Y108 (= Y107), N155 (= N154), D180 (= D179), S202 (= S201), T204 (= T203), K205 (= K204), V333 (= V365), S334 (≠ N366), R369 (= R401)

5x2wA Crystal structure of pseudomonas putida methionine gamma-lyase wild type with l-methionine intermediates (see paper)
41% identity, 99% coverage: 5:422/422 of query aligns to 6:390/392 of 5x2wA

query
sites
5x2wA

T

T

Q

R

A

A

V

I

H

H

A

H

G

G

Y

Y

S

D

P

P

D

Q

P

D

T

H

T

G

R

G

A

A

V

L

A

V

V

P

P

P

I

V

Y

Y

Q

Q

T

T

T

A

S

T

Y

F

A

T

F

F

D

P

D

T

T

V

Q

E

H

Y

G

G

A

A

D

A

L

C

F

F

D

A

L

G

K

E

V

Q

E

A

G

G

N

H

I

F

Y
|
Y

T

S

R
|
R

I

I

M

S

N

N

P

P

T

T

N

L

A

N

V

L

L

L

E

E

Q

A

R

R

V

M

A

A

A

S

M

L

E

E

G

G

G

G

I

E

G

A

G

G

L

L

A

A

V

L

A

A

S
|
S

G
|
G

M
|
M

A

G

A

A

I

I

T

T

Y

S

A

T

I

L

Q

W

A

T

I

L

T

L

R

R

A

P

G

G

D

D

N

E

I

V

V

L

S

L

T

G

S

N

R

T

L

L

Y
|
Y

G

G

G

C

T

T

Y

F

N

A

L

F

F

L

A

H

H

H

S

G

L

I

P

G

I

E

H

F

G

G

I

V

E

K

V

L

R

R

F

H

A

V

A

D

Q

M

D

A

D

D

Y

L

E

Q

R

A

L

L

E

E

A

A

Q

A

I

M

D

T

E

P

R

A

T

T

K

R

A

V

L

I

F

Y

C

F

E

E

S

S

V

P

G

A

N

N

P

P

S

N

G

M

E

H

V

M

V

A

D

D

V

I

E

A

R

G

L

V

A

A

E

K

I

I

A

A

H

R

R

K

H

H

G

G

I

A

P

T

L

V

M

V

V

V

D
|
D

N

N

T

T

V

Y

P

C

T

T

P

P

Y

Y

L

L

W

Q

R

R

P

P

I

L

D

E

N

L

G

G

A

A

D

D

I

L

V

V

I

V

H

H

S
|
S

L

A

T

T

K
|
K

Y

Y

M

L

G

S

G

G

H

H

G

G

T

D

T

I

V

T

G

A

G

G

V

I

I

V

V

V

D

-

S

-

G

-

K

-

F

-

P

-

W

-

A

-

D

-

H

-

G

-

D

-

R

-

F

-

P

-

M

-

M

-

V

-

E

-

P

-

D

-

P

-

S

-

Y

-

H

-

G

-

V

-

V

-

Y

-

T

-

E

-

D

-

M

-

G

G

E

S

A

Q

A

A

Y

L

I

V

T

D

R

R

C

I

R

R

V

L

V

Q

P

G

L

L

R

K

N

D

M

M

-

T

G

G

A

A

A

V

L

L

S

S

P

P

F

H

N

D

A

A

F

A

Q

L

L

L

L

M

Q

R

G

G

I

I

E

K

T

T

L

L

P

N

V

L

R

R

M

M

D

D

R

R

H

H

C

C

D

A

N

N

A

A

Q

Q

R

V

V

L

A

A

E

E

Y

F

L

L

Q

A

A

R

H

Q

P

P

R

Q

V

V

S

E

W

L

V

I

K

H

F

Y

A

P

G

G

L

L

E

A

D

S

S

F

P

P

Y

Q

K

Y

P

T

L

L

A

A

D

R

R

Q

Y

Q

M

M

G

S

G

-

R

Q

A

P

A

G

S

G

I

M

L

I

S

A

F

F

G

E

V

L

Q

K

G

G

G

G

F

I

E

G

A

A

G

G

A

R

R

R

F

F

I

M

D

N

A

A

L

L

Q

Q

L

L

I

F

T

S

R

R

L

A

V
|
V

N
x
S

I

L

G

G

D

D

A

A

K

E

S

S

L

L

A

A

C

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

S

L

Y

N

T

D

P

E

E

E

E

L

R

E

A

S

H

A

Y

G

G

V

I

S

S

R

E

D

G

L

L

V

V

R
|
R

L

L

S

S

I

V

G

G

L

L

E

E

H

D

V

I

D

D

D

D

I

L

L

L

A

A

D

D

I

V

D

Q

Q

Q

A

A

L

L

Q

K

A

A

active site: R55 (= R54), Y108 (= Y107), D180 (= D179), K205 (= K204)
binding (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]-4-(methylsulfanyl)but-2-enoic acid: Y53 (= Y52), R55 (= R54), S82 (= S81), G83 (= G82), M84 (= M83), Y108 (= Y107), D180 (= D179), S202 (= S201), K205 (= K204), V333 (= V365), S334 (≠ N366), R369 (= R401)

Query Sequence

>WP_011814939.1 NCBI__GCF_000015585.1:WP_011814939.1
MKLETQAVHAGYSPDPTTRAVAVPIYQTTSYAFDDTQHGADLFDLKVEGNIYTRIMNPTN
AVLEQRVAAMEGGIGGLAVASGMAAITYAIQAITRAGDNIVSTSRLYGGTYNLFAHSLPI
HGIEVRFAAQDDYERLEAQIDERTKALFCESVGNPSGEVVDVERLAEIAHRHGIPLMVDN
TVPTPYLWRPIDNGADIVIHSLTKYMGGHGTTVGGVIVDSGKFPWADHGDRFPMMVEPDP
SYHGVVYTEDMGEAAYITRCRVVPLRNMGAALSPFNAFQLLQGIETLPVRMDRHCDNAQR
VAEYLQAHPRVSWVKFAGLEDSPYKPLADRYMGGRAASILSFGVQGGFEAGARFIDALQL
ITRLVNIGDAKSLACHPASTTHRQLNDEELESAGVSRDLVRLSIGLEHVDDILADIDQAL
QA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory