SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011815179.1 NCBI__GCF_000015585.1:WP_011815179.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6yu6B Crystal structure of mhst in complex with l-leucine (see paper)
46% identity, 97% coverage: 11:448/453 of query aligns to 4:440/446 of 6yu6B

query
sites
6yu6B

E

Q

W

W

G

A

S

S

R

R

W

L

A

G

F

F

I

I

L

L

A

A

A

A

T

M

G

G

S
|
S

A
|
A

V

V

G
|
G

L

L

G
|
G

N

N

I

I

W

W

R

R

F

F

P

S

Y

Y

V

V

V

T

G

G

E

E

Y

N

G

G

G

G

G

A

A

A

F

F

I

L

L

L

I

V

Y

Y

L

L

A

G

C

F

I

I

L

A

A

L

I

I

G

G

I

I

P

P

V

I

M

V

M

L

A

A

E

E

I

F

L

T

I

I

G

G

R

R

R

R

G

A

R

Q

Q

S

S

D

P

A

I

V

N

G

T

S

M

F

R

E

A

K

L

L

P

-

A

-

E

-

F

-

G

A

A

P

G

G

R

K

W

P

W

W

Q

K

I

V

L

A

G

G

W

L

L

M

G

G

V

V

I

A

A

A

G

G

F

F

L

L

V

I

L

L

S

S

F

F

Y

Y

S

G

V

V

V

I

A

A

G

G

W

W

S

I

L

L

A

F

Y

Y

V

L

P

F

Y

N

T

Y

A

I

A

T

G

G

T

Q

F

L

T

W

G

S

T

A

E

P

T

A

S

E

Q

G

V

F

E

G

A

G

I

F

F

F

N

E

D

G

L

F

L

I

A

A

S

N

P

P

W

T

Q

L

M

P

L

L

F

F

W

W

H

Q

T

A

V

L

F

F

M

M

G

I

A

A

T

T

A

I

F

W

I

I

V

V

L

A

R

I

G

G

V

V

Q

K

N

K

G

G

L

I

E

E

R

R

A

S

V

N

R

K

L

I

L

L

M

M

P

P

I

L

L

L

F

G

L

V

L

L

L

L

L

I

V

A

M

L

V

A

G

I

Y

Y

G

S

M

L

G

T

A

L

G

G

A

G

F

A

V

K

E

E

A

G

M

L

D

A

F

F

M

L

F

F

S

S

P

P

D

D

F

W

S

S

A

A

I

L

G

K

G

D

D

P

A

G

V

V

-

Y

L

L

V

A

A

A

M

I

G

S

Q

Q

A

A

F

F

F
|
F

T
|
T

L

L

S
|
S

L

L

G

G

L
x
M

G

G

A

A

I

L

M

I

A

T

Y

Y

G

G

S

S

Y

Y

L

V

S

S

K

K

R

D

S

S

S

R

I

L

P

P

G

G

N

A

S

A

G

V

L

S

I

V

A

A

G

G

A

L

D

D

T

T

V

A

I

F

A

A

L

I

L

I

A

A

G

G

L

I

A

M

I

I

F

F

P

P

I

A

V

V

L

F

A

A

A

L

G

G

L

L

D

S

P

P

D

S

S

G

G

G

P

P

G

G

L

L

V

V

F

F

V

V

T

V

L

L

S

P

Y

D

G

I

F

F

G

D

Q

S

M

I

P

R

L

L

G

G

A

P

L

I

F

V

G

G

T

I

L

A

F

F

F

F

L

I

L

L

L

L

S

G

F

A

A

A

A

A

L

L

T

S

S

S

A

A

I

V

S

S

I
x
L

M

L

E

E

P

V

A

P

T

V

A

A

Y

Y

L

F

V

M

E

R

T

K

R

F

G

D

W

W

K

S

R

R

P

K

K

Q

A

A

A

A

A

I

A

T

V

L

A

G

A

V

A

I

A

I

W

T

L

L

V

L

G

G

I

I

G

P

S

S

L

S

L

L

A

S

F

F

N

G

V

V

W

L

A

G

G

E

F

V

T

T

-

I

I

I

A

P

G

G

L

L

N

N

F

I

Q

F

E

D

L

S

A

V

E

D

Y

F

L

I

S

A

T

S

S

S

V

V

M

F

L

L

P

P

L

L

G

G

G

G

L

M

L

I

M

I

A

A

L

L

F

F

A

I

G

G

W

W

V

G

L

W

P

K

R

T

S

S

V

D

T

A

T

L

E

A

E

E

L

S

A

D

L

L

P

T

D

D

W

S

V

V

Y

W

-

G

R

K

G

L

W

W

L

I

V

L

L

S

V

L

R

R

F

F

V

I

V

A

P

P

A

I

A

A

I

I

L

L

V

I

V

V

F

F

A

L

N

S

S

A

binding leucine: S18 (= S25), A19 (= A26), G21 (= G28), G23 (= G30), F223 (= F233), T224 (= T234), S226 (= S236), M229 (≠ L239), L321 (≠ I331)

6yu5A Crystal structure of mhst in complex with l-valine (see paper)
46% identity, 97% coverage: 11:448/453 of query aligns to 4:440/441 of 6yu5A

query
sites
6yu5A

E

Q

W

W

G

A

S

S

R

R

W

L

A

G

F

F

I

I

L

L

A

A

A

A

T

M

G

G

S
|
S

A
|
A

V

V

G

G

L

L

G
|
G

N

N

I

I

W

W

R

R

F

F

P

S

Y

Y

V

V

V

T

G

G

E

E

Y

N

G

G

G

G

G

A

A

A

F

F

I

L

L

L

I

V

Y

Y

L

L

A

G

C

F

I

I

L

A

A

L

I

I

G

G

I

I

P

P

V

I

M

V

M

L

A

A

E

E

I

F

L

T

I

I

G

G

R

R

R

R

G

A

R

Q

Q

S

S

D

P

A

I

V

N

G

T

S

M

F

R

E

A

K

L

L

P

-

A

-

E

-

F

-

G

A

A

P

G

G

R

K

W

P

W

W

Q

K

I

V

L

A

G

G

W

L

L

M

G

G

V

V

I

A

A

A

G

G

F

F

L

L

V

I

L

L

S

S

F

F

Y
|
Y

S

G

V

V

V

I

A

A

G

G

W

W

S

I

L

L

A

F

Y

Y

V

L

P

F

Y

N

T

Y

A

I

A

T

G

G

T

Q

F

L

T

W

G

S

T

A

E

P

T

A

S

E

Q

G

V

F

E

G

A

G

I

F

F

F

N

E

D

G

L

F

L

I

A

A

S

N

P

P

W

T

Q

L

M

P

L

L

F

F

W

W

H

Q

T

A

V

L

F

F

M

M

G

I

A

A

T

T

A

I

F

W

I

I

V

V

L

A

R

I

G

G

V

V

Q

K

N

K

G

G

L

I

E

E

R

R

A

S

V

N

R

K

L

I

L

L

M

M

P

P

I

L

L

L

F

G

L

V

L

L

L

L

L

I

V

A

M

L

V

A

G

I

Y

Y

G

S

M

L

G

T

A

L

G

G

A

G

F

A

V

K

E

E

A

G

M

L

D

A

F

F

M

L

F

F

S

S

P

P

D

D

F

W

S

S

A

A

I

L

G

K

G

D

D

P

A

G

V

V

-

Y

L

L

V

A

A

A

M

I

G

S

Q

Q

A

A

F

F

F
|
F

T
|
T

L

L

S
|
S

L

L

G

G

L

M

G

G

A

A

I

L

M

I

A

T

Y

Y

G

G

S

S

Y

Y

L

V

S

S

K

K

R

D

S

S

S

R

I

L

P

P

G

G

N

A

S

A

G

V

L

S

I

V

A

A

G

G

A

L

D

D

T

T

V

A

I

F

A

A

L

I

L

I

A

A

G

G

L

I

A

M

I

I

F

F

P

P

I

A

V

V

L

F

A

A

A

L

G

G

L

L

D

S

P

P

D

S

S

G

G

G

P

P

G

G

L

L

V

V

F

F

V

V

T

V

L

L

S

P

Y

D

G

I

F

F

G

D

Q

S

M

I

P

R

L

L

G

G

A

P

L

I

F

V

G

G

T

I

L

A

F

F

F

F

L

I

L

L

L

L

S

G

F

A

A

A

A

A

L

L

T

S

S
|
S

A

A

I

V

S

S

I

L

M

L

E

E

P

V

A

P

T

V

A

A

Y

Y

L

F

V

M

E

R

T

K

R

F

G

D

W

W

K

S

R

R

P

K

K

Q

A

A

A

A

A

I

A

T

V

L

A

G

A

V

A

I

A

I

W

T

L

L

V

L

G

G

I

I

G

P

S

S

L

S

L

L

A

S

F

F

N

G

V

V

W

L

A

G

G

E

F

V

T

T

-

I

I

I

A

P

G

G

L

L

N

N

F

I

Q

F

E

D

L

S

A

V

E

D

Y

F

L

I

S

A

T

S

S

S

V

V

M

F

L

L

P

P

L

L

G

G

G

G

L

M

L

I

M

I

A

A

L

L

F

F

A

I

G

G

W

W

V

G

L

W

P

K

R

T

S

S

V

D

T

A

T

L

E

A

E

E

L

S

A

D

L

L

P

T

D

D

W

S

V

V

Y

W

-

G

R

K

G

L

W

W

L

I

V

L

L

S

V

L

R

R

F

F

V

I

V

A

P

P

A

I

A

A

I

I

L

L

V

I

V

V

F

F

A

L

N

S

S

A

binding valine: S18 (= S25), A19 (= A26), G23 (= G30), Y101 (= Y112), F223 (= F233), T224 (= T234), S226 (= S236), S317 (= S327)

6yu3A Crystal structure of mhst in complex with l-phenylalanine (see paper)
46% identity, 97% coverage: 11:448/453 of query aligns to 4:440/441 of 6yu3A

query
sites
6yu3A

E

Q

W

W

G

A

S

S

R

R

W

L

A

G

F

F

I

I

L

L

A

A

A

A

T

M

G

G

S
|
S

A
|
A

V

V

G
|
G

L

L

G
|
G

N

N

I

I

W

W

R

R

F

F

P

S

Y

Y

V

V

V

T

G

G

E

E

Y

N

G

G

G

G

G

A

A

A

F

F

I

L

L

L

I

V

Y

Y

L

L

A

G

C

F

I

I

L

A

A

L

I

I

G

G

I

I

P

P

V

I

M

V

M

L

A

A

E

E

I

F

L

T

I

I

G

G

R

R

R

R

G

A

R

Q

Q

S

S

D

P

A

I

V

N

G

T

S

M

F

R

E

A

K

L

L

P

-

A

-

E

-

F

-

G

A

A

P

G

G

R

K

W

P

W

W

Q

K

I

V

L

A

G

G

W

L

L

M

G

G

V

V

I

A

A

A

G

G

F

F

L

L

V

I

L

L

S

S

F

F

Y
|
Y

S

G

V

V

V

I

A

A

G

G

W

W

S

I

L

L

A

F

Y

Y

V

L

P

F

Y

N

T

Y

A

I

A

T

G

G

T

Q

F

L

T

W

G

S

T

A

E

P

T

A

S

E

Q

G

V

F

E

G

A

G

I

F

F

F

N

E

D

G

L

F

L

I

A

A

S

N

P

P

W

T

Q

L

M

P

L

L

F

F

W

W

H

Q

T

A

V

L

F

F

M

M

G

I

A

A

T

T

A

I

F

W

I

I

V

V

L

A

R

I

G

G

V

V

Q

K

N

K

G

G

L

I

E

E

R

R

A

S

V

N

R

K

L

I

L

L

M

M

P

P

I

L

L

L

F

G

L

V

L

L

L

L

L

I

V

A

M

L

V

A

G

I

Y

Y

G

S

M

L

G

T

A

L

G

G

A

G

F

A

V

K

E

E

A

G

M

L

D

A

F

F

M

L

F

F

S

S

P

P

D

D

F

W

S

S

A

A

I

L

G

K

G

D

D

P

A

G

V

V

-

Y

L

L

V

A

A

A

M

I

G

S

Q

Q

A

A

F

F

F
|
F

T
|
T

L

L

S
|
S

L

L

G

G

L
x
M

G

G

A

A

I

L

M

I

A

T

Y

Y

G

G

S

S

Y

Y

L

V

S

S

K

K

R

D

S

S

S

R

I

L

P

P

G

G

N

A

S

A

G

V

L

S

I

V

A

A

G

G

A

L

D

D

T

T

V

A

I

F

A

A

L

I

L

I

A

A

G

G

L

I

A

M

I

I

F

F

P

P

I

A

V

V

L

F

A

A

A

L

G

G

L

L

D

S

P

P

D

S

S

G

G

G

P

P

G

G

L

L

V

V

F

F

V

V

T

V

L

L

S

P

Y

D

G

I

F

F

G

D

Q

S

M

I

P

R

L

L

G

G

A

P

L

I

F

V

G

G

T

I

L

A

F

F

F

F

L

I

L

L

L

L

S

G

F

A

A

A

A

A

L

L

T

S

S

S

A

A

I

V

S

S

I
x
L

M

L

E

E

P

V

A

P

T

V

A

A

Y

Y

L

F

V

M

E

R

T

K

R

F

G

D

W

W

K

S

R

R

P

K

K

Q

A

A

A

A

A

I

A

T

V

L

A

G

A

V

A

I

A

I

W

T

L

L

V

L

G

G

I

I

G

P

S

S

L

S

L

L

A

S

F

F

N

G

V

V

W

L

A

G

G

E

F

V

T

T

-

I

I

I

A

P

G

G

L

L

N

N

F

I

Q

F

E

D

L

S

A

V

E

D

Y

F

L

I

S

A

T

S

S

S

V

V

M

F

L

L

P

P

L

L

G

G

G

G

L

M

L

I

M

I

A

A

L

L

F

F

A

I

G

G

W

W

V

G

L

W

P

K

R

T

S

S

V

D

T

A

T

L

E

A

E

E

L

S

A

D

L

L

P

T

D

D

W

S

V

V

Y

W

-

G

R

K

G

L

W

W

L

I

V

L

L

S

V

L

R

R

F

F

V

I

V

A

P

P

A

I

A

A

I

I

L

L

V

I

V

V

F

F

A

L

N

S

S

A

binding phenylalanine: S18 (= S25), A19 (= A26), G21 (= G28), G23 (= G30), Y101 (= Y112), F223 (= F233), T224 (= T234), S226 (= S236), M229 (≠ L239), L321 (≠ I331)

6yu2A Crystal structure of mhst in complex with l-isoleucine (see paper)
46% identity, 97% coverage: 11:448/453 of query aligns to 4:440/441 of 6yu2A

query
sites
6yu2A

E

Q

W

W

G

A

S

S

R

R

W

L

A

G

F

F

I

I

L

L

A

A

A

A

T

M

G

G

S
|
S

A
|
A

V

V

G
|
G

L

L

G
|
G

N

N

I

I

W

W

R

R

F

F

P

S

Y

Y

V

V

V

T

G

G

E

E

Y

N

G

G

G

G

G

A

A

A

F

F

I

L

L

L

I

V

Y

Y

L

L

A

G

C

F

I

I

L

A

A

L

I

I

G

G

I

I

P

P

V

I

M

V

M

L

A

A

E

E

I

F

L

T

I

I

G

G

R

R

R

R

G

A

R

Q

Q

S

S

D

P

A

I

V

N

G

T

S

M

F

R

E

A

K

L

L

P

-

A

-

E

-

F

-

G

A

A

P

G

G

R

K

W

P

W

W

Q

K

I

V

L

A

G

G

W

L

L

M

G

G

V

V

I

A

A

A

G

G

F

F

L

L

V

I

L

L

S

S

F

F

Y
|
Y

S

G

V

V

V

I

A

A

G

G

W

W

S

I

L

L

A

F

Y

Y

V

L

P

F

Y

N

T

Y

A

I

A

T

G

G

T

Q

F

L

T

W

G

S

T

A

E

P

T

A

S

E

Q

G

V

F

E

G

A

G

I

F

F

F

N

E

D

G

L

F

L

I

A

A

S

N

P

P

W

T

Q

L

M

P

L

L

F

F

W

W

H

Q

T

A

V

L

F

F

M

M

G

I

A

A

T

T

A

I

F

W

I

I

V

V

L

A

R

I

G

G

V

V

Q

K

N

K

G

G

L

I

E

E

R

R

A

S

V

N

R

K

L

I

L

L

M

M

P

P

I

L

L

L

F

G

L

V

L

L

L

L

L

I

V

A

M

L

V

A

G

I

Y

Y

G

S

M

L

G

T

A

L

G

G

A

G

F

A

V

K

E

E

A

G

M

L

D

A

F

F

M

L

F

F

S

S

P

P

D

D

F

W

S

S

A

A

I

L

G

K

G

D

D

P

A

G

V

V

-

Y

L

L

V

A

A

A

M

I

G

S

Q

Q

A

A

F

F

F
|
F

T

T

L

L

S

S

L

L

G

G

L
x
M

G

G

A

A

I

L

M

I

A

T

Y

Y

G

G

S

S

Y

Y

L

V

S

S

K

K

R

D

S

S

S

R

I

L

P

P

G

G

N

A

S

A

G

V

L

S

I

V

A

A

G

G

A

L

D

D

T

T

V

A

I

F

A

A

L

I

L

I

A

A

G

G

L

I

A

M

I

I

F

F

P

P

I

A

V

V

L

F

A

A

A

L

G

G

L

L

D

S

P

P

D

S

S

G

G

G

P

P

G

G

L

L

V

V

F

F

V

V

T

V

L

L

S

P

Y

D

G

I

F

F

G

D

Q

S

M

I

P

R

L

L

G

G

A

P

L

I

F

V

G

G

T

I

L

A

F

F

F

F

L

I

L

L

L

L

S

G

F

A

A

A

A

A

L

L

T

S

S
|
S

A

A

I

V

S

S

I

L

M

L

E

E

P

V

A

P

T

V

A

A

Y

Y

L

F

V

M

E

R

T

K

R

F

G

D

W

W

K

S

R

R

P

K

K

Q

A

A

A

A

A

I

A

T

V

L

A

G

A

V

A

I

A

I

W

T

L

L

V

L

G

G

I

I

G

P

S

S

L

S

L

L

A

S

F

F

N

G

V

V

W

L

A

G

G

E

F

V

T

T

-

I

I

I

A

P

G

G

L

L

N

N

F

I

Q

F

E

D

L

S

A

V

E

D

Y

F

L

I

S

A

T

S

S

S

V

V

M

F

L

L

P

P

L

L

G

G

G

G

L

M

L

I

M

I

A

A

L

L

F

F

A

I

G

G

W

W

V

G

L

W

P

K

R

T

S

S

V

D

T

A

T

L

E

A

E

E

L

S

A

D

L

L

P

T

D

D

W

S

V

V

Y

W

-

G

R

K

G

L

W

W

L

I

V

L

L

S

V

L

R

R

F

F

V

I

V

A

P

P

A

I

A

A

I

I

L

L

V

I

V

V

F

F

A

L

N

S

S

A

binding isoleucine: S18 (= S25), A19 (= A26), G21 (= G28), G23 (= G30), Y101 (= Y112), F223 (= F233), M229 (≠ L239), S317 (= S327)

4us3A Crystal structure of the bacterial nss member mhst in an occluded inward-facing state (see paper)
46% identity, 97% coverage: 11:448/453 of query aligns to 4:440/441 of 4us3A

query
sites
4us3A

E

Q

W

W

G

A

S

S

R

R

W

L

A

G

F

F

I

I

L

L

A

A

A

A

T

M

G
|
G

S
|
S

A
|
A

V
|
V

G
|
G

L
|
L

G
|
G

N
|
N

I

I

W

W

R

R

F

F

P

S

Y

Y

V

V

V

T

G

G

E

E

Y

N

G

G

G

G

G

A

A

A

F

F

I

L

L

L

I

V

Y

Y

L

L

A

G

C

F

I

I

L

A

A

L

I

I

G

G

I

I

P

P

V

I

M

V

M

L

A

A

E

E

I

F

L

T

I

I

G

G

R

R

R

R

G

A

R

Q

Q

S

S

D

P

A

I

V

N

G

T

S

M

F

R

E

A

K

L

L

P

-

A

-

E

-

F

-

G

A

A

P

G

G

R

K

W

P

W

W

Q

K

I

V

L

A

G

G

W

L

L

M

G

G

V

V

I

A

A

A

G

G

F

F

L

L

V

I

L

L

S

S

F

F

Y
|
Y

S

G

V

V

V

I

A

A

G

G

W

W

S

I

L

L

A

F

Y

Y

V

L

P

F

Y

N

T

Y

A

I

A

T

G

G

T

Q

F

L

T

W

G

S

T

A

E

P

T

A

S

E

Q

G

V

F

E

G

A

G

I

F

F

F

N

E

D

G

L

F

L

I

A

A

S

N

P

P

W

T

Q

L

M

P

L

L

F

F

W

W

H

Q

T

A

V

L

F

F

M

M

G

I

A

A

T

T

A

I

F

W

I

I

V

V

L

A

R

I

G

G

V

V

Q

K

N

K

G

G

L

I

E

E

R

R

A

S

V

N

R

K

L

I

L

L

M

M

P

P

I

L

L

L

F

G

L

V

L

L

L

L

L

I

V

A

M

L

V

A

G

I

Y

Y

G

S

M

L

G

T

A

L

G

G

A

G

F

A

V

K

E

E

A

G

M

L

D

A

F

F

M

L

F

F

S

S

P

P

D

D

F

W

S

S

A

A

I

L

G

K

G

D

D

P

A

G

V

V

-

Y

L

L

V

A

A

A

M

I

G

S

Q

Q

A

A

F

F

F
|
F

T
|
T

L

L

S
|
S

L

L

G

G

L
x
M

G

G

A

A

I

L

M

I

A

T

Y

Y

G

G

S

S

Y

Y

L

V

S

S

K

K

R

D

S

S

S

R

I

L

P

P

G

G

N

A

S

A

G

V

L

S

I

V

A

A

G

G

A

L

D
|
D

T

T

V

A

I

F

A

A

L

I

L

I

A

A

G

G

L

I

A

M

I

I

F

F

P

P

I

A

V

V

L

F

A

A

A

L

G

G

L

L

D

S

P

P

D

S

S

G

G

G

P

P

G

G

L

L

V

V

F

F

V

V

T

V

L

L

S

P

Y

D

G

I

F

F

G

D

Q

S

M

I

P

R

L

L

G

G

A

P

L

I

F

V

G

G

T

I

L

A

F

F

F

F

L

I

L

L

L

L

S

G

F

A

A
|
A

A

A

L

L

T
x
S

S
|
S

A

A

I

V

S
|
S

I
x
L

M

L

E

E

P

V

A

P

T

V

A

A

Y

Y

L

F

V

M

E

R

T

K

R

F

G

D

W

W

K

S

R

R

P

K

K

Q

A

A

A

A

A

I

A

T

V

L

A

G

A

V

A

I

A

I

W

T

L

L

V

L

G

G

I

I

G

P

S

S

L

S

L

L

A

S

F

F

N

G

V

V

W

L

A

G

G

E

F

V

T

T

-

I

I

I

A

P

G

G

L

L

N

N

F

I

Q

F

E

D

L

S

A

V

E

D

Y

F

L

I

S

A

T

S

S

S

V

V

M

F

L

L

P

P

L

L

G

G

G

G

L

M

L

I

M

I

A

A

L

L

F

F

A

I

G

G

W

W

V

G

L

W

P

K

R

T

S

S

V

D

T

A

T

L

E

A

E

E

L

S

A

D

L

L

P

T

D

D

W

S

V

V

Y

W

-

G

R

K

G

L

W

W

L

I

V

L

L

S

V

L

R

R

F

F

V

I

V

A

P

P

A

I

A

A

I

I

L

L

V

I

V

V

F

F

A

L

N

S

S

A

binding sodium ion: G17 (= G24), A19 (= A26), V20 (= V27), V20 (= V27), G21 (= G28), N24 (= N31), T224 (= T234), D256 (= D266), A313 (= A323), S316 (≠ T326), S317 (= S327)
binding tryptophan: S18 (= S25), A19 (= A26), L22 (= L29), G23 (= G30), Y101 (= Y112), F223 (= F233), T224 (= T234), S226 (= S236), M229 (≠ L239), S320 (= S330), L321 (≠ I331)

6yu7A Crystal structure of mhst in complex with l-tyrosine (see paper)
46% identity, 97% coverage: 11:448/453 of query aligns to 4:440/442 of 6yu7A

query
sites
6yu7A

E

Q

W

W

G

A

S

S

R

R

W

L

A

G

F

F

I

I

L

L

A

A

A

A

T

M

G

G

S
|
S

A
|
A

V

V

G
|
G

L

L

G
|
G

N

N

I

I

W

W

R

R

F

F

P

S

Y

Y

V

V

V

T

G

G

E

E

Y

N

G

G

G

G

G

A

A

A

F

F

I

L

L

L

I

V

Y

Y

L

L

A

G

C

F

I

I

L

A

A

L

I

I

G

G

I

I

P

P

V

I

M

V

M

L

A

A

E

E

I

F

L

T

I

I

G

G

R

R

R

R

G

A

R

Q

Q

S

S

D

P

A

I

V

N

G

T

S

M

F

R

E

A

K

L

L

P

-

A

-

E

-

F

-

G

A

A

P

G

G

R

K

W

P

W

W

Q

K

I

V

L

A

G

G

W

L

L

M

G

G

V

V

I

A

A

A

G

G

F

F

L

L

V

I

L

L

S

S

F

F

Y
|
Y

S

G

V

V

V

I

A

A

G

G

W

W

S

I

L

L

A

F

Y

Y

V

L

P

F

Y

N

T

Y

A

I

A

T

G

G

T

Q

F

L

T

W

G

S

T

A

E

P

T

A

S

E

Q

G

V

F

E

G

A

G

I

F

F

F

N

E

D

G

L

F

L

I

A

A

S

N

P

P

W

T

Q

L

M

P

L

L

F

F

W

W

H

Q

T

A

V

L

F

F

M

M

G

I

A

A

T

T

A

I

F

W

I

I

V

V

L

A

R

I

G

G

V

V

Q

K

N

K

G

G

L

I

E

E

R

R

A

S

V

N

R

K

L

I

L

L

M

M

P

P

I

L

L

L

F

G

L

V

L

L

L

L

L

I

V

A

M

L

V

A

G

I

Y

Y

G

S

M

L

G

T

A

L

G

G

A

G

F

A

V

K

E

E

A

G

M

L

D

A

F

F

M

L

F

F

S

S

P

P

D

D

F

W

S

S

A

A

I

L

G

K

G

D

D

P

A

G

V

V

-

Y

L

L

V

A

A

A

M

I

G

S

Q

Q

A

A

F

F

F
|
F

T
|
T

L

L

S
|
S

L

L

G

G

L
x
M

G

G

A

A

I

L

M

I

A

T

Y

Y

G

G

S

S

Y

Y

L

V

S

S

K

K

R

D

S

S

S

R

I

L

P

P

G

G

N

A

S

A

G

V

L

S

I

V

A

A

G

G

A

L

D

D

T

T

V

A

I

F

A

A

L

I

L

I

A

A

G

G

L

I

A

M

I

I

F

F

P

P

I

A

V

V

L

F

A

A

A

L

G

G

L

L

D

S

P

P

D

S

S

G

G

G

P

P

G

G

L

L

V

V

F

F

V

V

T

V

L

L

S

P

Y

D

G

I

F

F

G

D

Q

S

M

I

P

R

L

L

G

G

A

P

L

I

F

V

G

G

T

I

L

A

F

F

F

F

L

I

L

L

L

L

S

G

F

A

A

A

A

A

L

L

T

S

S

S

A

A

I

V

S

S

I
x
L

M

L

E

E

P

V

A

P

T

V

A

A

Y

Y

L

F

V

M

E

R

T

K

R

F

G

D

W

W

K

S

R

R

P

K

K

Q

A

A

A

A

A

I

A

T

V

L

A

G

A

V

A

I

A

I

W

T

L

L

V

L

G

G

I

I

G

P

S

S

L

S

L

L

A

S

F

F

N

G

V

V

W

L

A

G

G

E

F

V

T

T

-

I

I

I

A

P

G

G

L

L

N

N

F

I

Q

F

E

D

L

S

A

V

E

D

Y

F

L

I

S

A

T

S

S

S

V

V

M

F

L

L

P

P

L

L

G

G

G

G

L

M

L

I

M

I

A

A

L

L

F

F

A

I

G

G

W

W

V

G

L

W

P

K

R

T

S

S

V

D

T

A

T

L

E

A

E

E

L

S

A

D

L

L

P

T

D

D

W

S

V

V

Y

W

-

G

R

K

G

L

W

W

L

I

V

L

L

S

V

L

R

R

F

F

V

I

V

A

P

P

A

I

A

A

I

I

L

L

V

I

V

V

F

F

A

L

N

S

S

A

binding tyrosine: S18 (= S25), A19 (= A26), G21 (= G28), G23 (= G30), Y101 (= Y112), F223 (= F233), T224 (= T234), S226 (= S236), M229 (≠ L239), L321 (≠ I331)

6yu4A Crystal structure of mhst in complex with l-4f-phenylalanine (see paper)
46% identity, 97% coverage: 11:448/453 of query aligns to 4:440/442 of 6yu4A

query
sites
6yu4A

E

Q

W

W

G

A

S

S

R

R

W

L

A

G

F

F

I

I

L

L

A

A

A

A

T

M

G

G

S
|
S

A
|
A

V

V

G
|
G

L
|
L

G
|
G

N

N

I

I

W

W

R

R

F

F

P

S

Y

Y

V

V

V

T

G

G

E

E

Y

N

G

G

G

G

G

A

A

A

F

F

I

L

L

L

I

V

Y

Y

L

L

A

G

C

F

I

I

L

A

A

L

I

I

G

G

I

I

P

P

V

I

M

V

M

L

A

A

E

E

I

F

L

T

I

I

G

G

R

R

R

R

G

A

R

Q

Q

S

S

D

P

A

I

V

N

G

T

S

M

F

R

E

A

K

L

L

P

-

A

-

E

-

F

-

G

A

A

P

G

G

R

K

W

P

W

W

Q

K

I

V

L

A

G

G

W

L

L

M

G

G

V

V

I

A

A

A

G

G

F

F

L

L

V

I

L

L

S

S

F

F

Y
|
Y

S

G

V

V

V

I

A

A

G

G

W

W

S

I

L

L

A

F

Y

Y

V

L

P

F

Y

N

T

Y

A

I

A

T

G

G

T

Q

F

L

T

W

G

S

T

A

E

P

T

A

S

E

Q

G

V

F

E

G

A

G

I

F

F

F

N

E

D

G

L

F

L

I

A

A

S

N

P

P

W

T

Q

L

M

P

L

L

F

F

W

W

H

Q

T

A

V

L

F

F

M

M

G

I

A

A

T

T

A

I

F

W

I

I

V

V

L

A

R

I

G

G

V

V

Q

K

N

K

G

G

L

I

E

E

R

R

A

S

V

N

R

K

L

I

L

L

M

M

P

P

I

L

L

L

F

G

L

V

L

L

L

L

L

I

V

A

M

L

V

A

G

I

Y

Y

G

S

M

L

G

T

A

L

G

G

A

G

F

A

V

K

E

E

A

G

M

L

D

A

F

F

M

L

F

F

S

S

P

P

D

D

F

W

S

S

A

A

I

L

G

K

G

D

D

P

A

G

V

V

-

Y

L

L

V

A

A

A

M

I

G

S

Q

Q

A

A

F

F

F
|
F

T
|
T

L

L

S
|
S

L

L

G

G

L
x
M

G

G

A

A

I

L

M

I

A

T

Y

Y

G

G

S

S

Y

Y

L

V

S

S

K

K

R

D

S

S

S

R

I

L

P

P

G

G

N

A

S

A

G

V

L

S

I

V

A

A

G

G

A

L

D

D

T

T

V

A

I

F

A

A

L

I

L

I

A

A

G

G

L

I

A

M

I

I

F

F

P

P

I

A

V

V

L

F

A

A

A

L

G

G

L

L

D

S

P

P

D

S

S

G

G

G

P

P

G

G

L

L

V

V

F

F

V

V

T

V

L

L

S

P

Y

D

G

I

F

F

G

D

Q

S

M

I

P

R

L

L

G

G

A

P

L

I

F

V

G

G

T

I

L

A

F

F

F

F

L

I

L

L

L

L

S

G

F

A

A

A

A

A

L

L

T

S

S

S

A

A

I

V

S

S

I
x
L

M

L

E

E

P

V

A

P

T

V

A

A

Y

Y

L

F

V

M

E

R

T

K

R

F

G

D

W

W

K

S

R

R

P

K

K

Q

A

A

A

A

A

I

A

T

V

L

A

G

A

V

A

I

A

I

W

T

L

L

V

L

G

G

I

I

G

P

S

S

L

S

L

L

A

S

F

F

N

G

V

V

W

L

A

G

G

E

F

V

T

T

-

I

I

I

A

P

G

G

L

L

N

N

F

I

Q

F

E

D

L

S

A

V

E

D

Y

F

L

I

S

A

T

S

S

S

V

V

M

F

L

L

P

P

L

L

G

G

G

G

L

M

L

I

M

I

A

A

L

L

F

F

A

I

G

G

W

W

V

G

L

W

P

K

R

T

S

S

V

D

T

A

T

L

E

A

E

E

L

S

A

D

L

L

P

T

D

D

W

S

V

V

Y

W

-

G

R

K

G

L

W

W

L

I

V

L

L

S

V

L

R

R

F

F

V

I

V

A

P

P

A

I

A

A

I

I

L

L

V

I

V

V

F

F

A

L

N

S

S

A

binding 4-fluoro-l-phenylalanine: S18 (= S25), A19 (= A26), G21 (= G28), L22 (= L29), G23 (= G30), Y101 (= Y112), F223 (= F233), T224 (= T234), S226 (= S236), M229 (≠ L239), L321 (≠ I331)

4us4A Crystal structure of the bacterial nss member mhst in an occluded inward-facing state (lipidic cubic phase form) (see paper)
46% identity, 95% coverage: 18:448/453 of query aligns to 2:431/433 of 4us4A

query
sites
4us4A

F

F

I

I

L

L

A

A

A

A

T

M

G
|
G

S
|
S

A
|
A

V
|
V

G
|
G

L

L

G
|
G

N
|
N

I

I

W

W

R

R

F

F

P

S

Y

Y

V

V

V

T

G

G

E

E

Y

N

G

G

G

G

G

A

A

A

F

F

I

L

L

L

I

V

Y

Y

L

L

A

G

C

F

I

I

L

A

A

L

I

I

G

G

I

I

P

P

V

I

M

V

M

L

A

A

E

E

I

F

L

T

I

I

G

G

R

R

R

R

G

A

R

Q

Q

S

S

D

P

A

I

V

N

G

T

S

M

F

R

E

A

K

L

L

P

-

A

-

E

-

F

-

G

A

A

P

G

G

R

K

W

P

W

W

Q

K

I

V

L

A

G

G

W

L

L

M

G

G

V

V

I

A

A

A

G

G

F

F

L

L

V

I

L

L

S

S

F

F

Y
|
Y

S

G

V

V

V

I

A

A

G

G

W

W

S

I

L

L

A

F

Y

Y

V

L

P

F

Y

N

T

Y

A

I

A

T

G

G

T

Q

F

L

T

W

G

S

T

A

E

P

T

A

S

E

Q

G

V

F

E

G

A

G

I

F

F

F

N

E

D

G

L

F

L

I

A

A

S

N

P

P

W

T

Q

L

M

P

L

L

F

F

W

W

H

Q

T

A

V

L

F

F

M

M

G

I

A

A

T

T

A

I

F

W

I

I

V

V

L

A

R

I

G

G

V

V

Q

K

N

K

G

G

L

I

E

E

R

R

A

S

V

N

R

K

L

I

L

L

M

M

P

P

I

L

L

L

F

G

L

V

L

L

L

L

L

I

V
x
A

M

L

V

A

G

I

Y

Y

G

S

M

L

G
x
T

A

L

G

G

A

G

F

A

V

K

E

E

A

G

M

L

D

A

F

F

M

L

F

F

S

S

P

P

D

D

F

W

S

S

A

A

I

L

G

K

G

D

D

P

A

G

V

V

-

Y

L

L

V

A

A

A

M

I

G

S

Q

Q

A

A

F

F

F
|
F

T
|
T

L

L

S
|
S

L

L

G

G

L
x
M

G

G

A

A

I

L

M

I

A

T

Y

Y

G

G

S

S

Y

Y

L

V

S

S

K

K

R

D

S

S

S

R

I

L

P

P

G

G

N

A

S

A

G

V

L

S

I

V

A

A

G

G

A

L

D
|
D

T

T

V

A

I

F

A

A

L

I

L

I

A

A

G

G

L

I

A

M

I

I

F

F

P

P

I

A

V

V

L

F

A

A

A

L

G

G

L

L

D

S

P

P

D

S

S

G

G

G

P

P

G

G

L

L

V

V

F

F

V

V

T

V

L

L

S

P

Y

D

G

I

F

F

G

D

Q

S

M
x
I

P
x
R

L
|
L

G

G

A

P

L

I

F

V

G

G

T

I

L

A

F

F

F

F

L

I

L

L

L

L

S

G

F

A

A
|
A

A

A

L

L

T
x
S

S
|
S

A

A

I

V

S

S

I
x
L

M

L

E

E

P

V

A

P

T

V

A

A

Y

Y

L

F

V

M

E

R

T

K

R

F

G

D

W

W

K

S

R

R

P

K

K

Q

A

A

A

A

A

I

A

T

V

L

A

G

A

V

A

I

A

I

W

T

L

L

V

L

G

G

I

I

G

P

S

S

L

S

L

L

A

S

F

F

N

G

V

V

W

L

A

G

G

E

F

V

T

T

-

I

I

I

A

P

G

G

L

L

N

N

F

I

Q

F

E

D

L

S

A

V

E

D

Y

F

L

I

S

A

T

S

S

S

V

V

M

F

L

L

P

P

L

L

G

G

G

G

L

M

L

I

M

I

A

A

L

L

F

F

A

I

G

G

W

W

V

G

L

W

P

K

R

T

S

S

V

D

T

A

T

L

E

A

E

E

L

S

A

D

L

L

P

T

D

D

W

S

V

V

Y

W

-

G

R

K

G

L

W

W

L

I

V

L

L

S

V

L

R

R

F

F

V

I

V

A

P

P

A

I

A

A

I

I

L

L

V

I

V

V

F

F

A

L

N

S

S

A

binding (2s)-2,3-dihydroxypropyl(7z)-pentadec-7-enoate: A173 (≠ V193), T180 (≠ G200), I287 (≠ M306), R288 (≠ P307), L289 (= L308)
binding sodium ion: G8 (= G24), S9 (= S25), A10 (= A26), V11 (= V27), V11 (= V27), N15 (= N31), T215 (= T234), D247 (= D266), A304 (= A323), S307 (≠ T326), S308 (= S327)
binding tryptophan: S9 (= S25), A10 (= A26), G12 (= G28), G14 (= G30), Y92 (= Y112), F214 (= F233), T215 (= T234), S217 (= S236), M220 (≠ L239), L312 (≠ I331)

3gwwA Leucine transporter leut in complex with s-fluoxetine (see paper)
33% identity, 80% coverage: 11:374/453 of query aligns to 3:399/501 of 3gwwA

query
sites
3gwwA

E

H

W

W

G

A

S

T

R

R

W

L

A

G

F

L

I

I

L

L

A

A

A

M

T

A

G

G

S

N

A
|
A

V

V

G
|
G

L
|
L

G
|
G

N

N

I

F

W

L

R
|
R

F

F

P

P

Y

V

V

Q

V

A

G

A

E

E

Y

N

G

G

G

G

G

G

A

A

F

F

I

M

L

I

I

P

Y

Y

L

I

A

I

C

A

I

F

L

L

A

L

I

V

G

G

I

I

P

P

V

L

M

M

M

W

A

I

E

E

I

W

L

A

I

M

G

G

R

R

R

Y

G

G

R

G

Q

A

S

Q

P

G

I

H

N

G

T

T

M

T

R

P

A

A

L

I

P

F

A

Y

E

L

F

L

G

W

A

R

G

N

R

R

W

F

W

A

Q

K

I

I

L

L

G

G

W

V

L

F

G

G

V

L

I

W

A

I

G

P

F

L

L

V

V

V

L

A

S

I

F

Y

Y
|
Y

S

V

V

Y

V

I

A

E

G

S

W

W

S

T

L

L

A

G

Y

F

V

A

P

I

Y

K

T

F

A

L

A

V

G

G

T

L

F

V

T

P

G

E

T

P

E

P

T

P

S

D

Q

S

V

I

E

L

A

R

I

P

F

F

N

K

D

E

L

F

L

L

A

Y

S

S

P

Y

W

I

Q

G

M

V

-

P

-

K

-

G

-

D

-

E

-

P

-

I

-

L

-

K

-

P

-

S

L

L

F

F

W

A

H

Y

T

I

V

V

F

F

-

L

-

I

-

T

M

M

G

F

A

I

T

N

A

V

F

S

I

I

V

L

L

I

R

R

G

G

V

I

Q

S

N

K

G

G

L

I

E

E

R

R

A

F

V

A

R

K

L

I

L

A

M

M

P

P

I

T

L

L

F

F

L

I

L

L

L

A

L

V

V

F

M

L

V

V

G

I

Y

R

-

V

-

F

-

L

-

L

G

E

M

T

G

P

A

N

G

G

A

T

F

A

V

A

E

D

A

G

M

L

D

N

F

F

M

L

F

W

S

T

P

P

D

D

F

F

S

E

A

K

I

L

G

K

G

D

D

P

A

G

V

V

-

W

L

I

V

A

A

A

M

V

G

G

Q

Q

A

I

F

F

F
|
F

T
|
T

L

L

S
|
S

L

L

G

G

L
x
F

G

G

A

A

I

I

M

I

A

T

Y

Y

G

A

S

S

Y

Y

L

V

S

R

K

K

R

D

S

Q

S

D

I

I

P

V

G

-

N

L

S

S

G

G

L

L

I

T

A

A

G

A

-

T

-

L

-

N

-

E

-

K

A

A

D

E

T

V

V

I

-

L

-

G

-

G

-

S

I

I

A

S

L

I

L

P

A

A

G

A

L

V

A

A

I

F

F

F

P

G

I

V

V

A

L

N

A

A

A

V

G

A

L

I

D

A

P

K

D

A

S

G

G
x
A

P
x
F

G

N

L

L

V

G

F

F

V

I

T

T

L

L

S

P

Y

A

G

I

F

F

G

S

Q

Q

M

T

P

A

L

G

G

G

A

T

L

F

F

L

G

G

T

F

L

L

F

W

F

F

L

F

L

L

L

L

S

F

F

F

A

A

A

G

L

L

T

T

S

S

A

S

I

I

S

A

I
|
I

M

M

E

Q

P

P

A

M

T

I

A

A

Y

F

L

L

V

E

E

D

T

E

R

L

G

K

W

L

K

S

R

R

P

K

K

H

A

A

-

V

-

L

-

W

-

T

A

A

A

A

A

I

V

V

A

F

A

F

A

S

A

A

W

H

L

L

V

V

G

M

I

F

-

L

-

N

G

K

S

S

L

L

L
x
D

A

E

F

M

N
x
D

V

F

W

W

A

A

G

G

binding leucine: A18 (= A26), G20 (= G28), G22 (= G30), Y104 (= Y112), F244 (= F233), T245 (= T234), S247 (= S236), F250 (≠ L239), I350 (= I331)
binding (3S)-N-methyl-3-phenyl-3-[4-(trifluoromethyl)phenoxy]propan-1-amine: L21 (= L29), G22 (= G30), R26 (= R34), Y104 (= Y112), A310 (≠ G291), F311 (≠ P292), D392 (≠ L367), D395 (≠ N370)

3mpnA F177r1 mutant of leut (see paper)
33% identity, 80% coverage: 11:374/453 of query aligns to 3:402/505 of 3mpnA

query
sites
3mpnA

E

H

W

W

G

A

S

T

R

R

W

L

A

G

F

L

I

I

L

L

A

A

A

M

T

A

G

G

S
x
N

A
|
A

V

V

G
|
G

L
|
L

G
|
G

N

N

I

F

W

L

R

R

F

F

P

P

Y

V

V

Q

V

A

G

A

E

E

Y

N

G

G

G

G

G

G

A

A

F

F

I

M

L

I

I

P

Y

Y

L

I

A

I

C

A

I

F

L

L

A

L

I

V

G

G

I

I

P

P

V

L

M

M

M

W

A

I

E

E

I

W

L

A

I

M

G

G

R

R

R

Y

G

G

R

G

Q

A

S

Q

P

G

I

H

N

G

T

T

M

T

R

P

A

A

L

I

P

F

A

Y

E

L

F

L

G

W

A

R

G

N

R

R

W

F

W

A

Q

K

I

I

L

L

G

G

W

V

L

F

G

G

V

L

I

W

A

I

G

P

F

L

L

V

V

V

L

A

S

I

F

Y

Y
|
Y

S

V

V

Y

V

I

A

E

G

S

W

W

S

T

L

L

A

G

Y

F

V

A

P

I

Y

K

T

F

A

L

A

V

G

G

T

L

F

V

T

P

G

E

T

P

E

P

T

P

S

T

Q

D

V

P

E

D

A

S

I

I

-

L

-

R

-

P

F

F

N

K

D

E

L

F

L

L

A

Y

S

S

P

Y

W

I

Q

G

M

V

-

P

-

K

-

G

-

D

-

E

-

P

-

I

-

L

-

K

-

P

-

S

L

L

F

F

W

A

H

Y

T

I

V

V

F

F

-

L

-

I

-

T

M

M

G
x
C

A

I

T

N

A

V

F

S

I

I

V

L

L

I

R

R

G

G

V

I

Q

S

N

K

G

G

L

I

E

E

R

R

A

F

V

A

R

K

L

I

L

A

M

M

P

P

I

T

L

L

F

F

L

I

L

L

L

A

L

V

V

F

M

L

V

V

G

I

Y

R

-

V

-

F

-

L

-

L

G

E

M

T

G

P

A

N

G

G

A

T

F

A

V

A

E

D

A

G

M

L

D

N

F

F

M

L

F

W

S

T

P

P

D

D

F

F

S

E

A

K

I

L

G

K

G

D

D

P

A

G

V

V

-

W

L

I

V

A

A

A

M

V

G

G

Q

Q

A

I

F

F

F
|
F

T
|
T

L

L

S
|
S

L

L

G

G

L
x
F

G

G

A

A

I

I

M

I

A

T

Y

Y

G

A

S

S

Y

Y

L

V

S

R

K

K

R

D

S

Q

S

D

I

I

P

V

G

-

N

L

S

S

G

G

L

L

I

T

A

A

G

A

-

T

-

L

-

N

-

E

-

K

A

A

D

E

T

V

V

I

-

L

-

G

-

G

-

S

I

I

A

S

L

I

L

P

A

A

G

A

L

V

A

A

I

F

F

F

P

G

I

V

V

A

L

N

A

A

A

V

G

A

L

I

D

A

P

K

D

A

S

G

G

A

P

F

G

N

L

L

V

G

F

F

V

I

T

T

L

L

S

P

Y

A

G

I

F

F

G

S

Q

Q

M

T

P

A

L

G

G

G

A

T

L

F

F

L

G

G

T

F

L

L

F

W

F

F

L

F

L

L

L

L

S

F

F

F

A

A

A

G

L

L

T

T

S
|
S

A

S

I

I

S

A

I

I

M

M

E

Q

P

P

A

M

T

I

A

A

Y

F

L

L

V

E

E

D

T

E

R

L

G

K

W

L

K

S

R

R

P

K

K

H

A

A

-

V

-

L

-

W

-

T

A
|
A

A

A

A

I

V

V

A

F

A

F

A

S

A

A

W

H

L

L

V

V

G

M

I

F

-

L

-

N

G

K

S

S

L

L

L

D

A

E

F

M

N

D

V

F

W

W

A

A

G

G

binding leucine: N17 (≠ S25), A18 (= A26), G20 (= G28), L21 (= L29), G22 (= G30), Y104 (= Y112), F247 (= F233), T248 (= T234), S250 (= S236), F253 (≠ L239), S349 (= S327)
binding S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate: C171 (≠ G162), A377 (= A351)

2q6hA Crystal structure analysis of leut complexed with l-leucine, sodium, and clomipramine (see paper)
33% identity, 80% coverage: 11:374/453 of query aligns to 5:404/512 of 2q6hA

query
sites
2q6hA

E

H

W

W

G

A

S

T

R

R

W

L

A

G

F

L

I

I

L

L

A

A

A

M

T

A

G
|
G

S

N

A
|
A

V
|
V

G
|
G

L

L

G
|
G

N
|
N

I

F

W

L

R
|
R

F

F

P

P

Y

V

V
x
Q

V

A

G

A

E

E

Y

N

G

G

G

G

G

G

A

A

F

F

I

M

L

I

I

P

Y

Y

L

I

A

I

C

A

I

F

L

L

A

L

I

V

G

G

I

I

P

P

V

L

M

M

M

W

A

I

E

E

I

W

L

A

I

M

G

G

R

R

R

Y

G

G

R

G

Q

A

S

Q

P

G

I

H

N

G

T

T

M

T

R

P

A

A

L

I

P

F

A

Y

E

L

F

L

G

W

A

R

G

N

R

R

W

F

W

A

Q

K

I

I

L

L

G

G

W

V

L

F

G

G

V

L

I

W

A

I

G

P

F

L

L

V

V

V

L

A

S

I

F

Y

Y
|
Y

S

V

V

Y

V

I

A

E

G

S

W

W

S

T

L

L

A

G

Y

F

V

A

P

I

Y

K

T

F

A

L

A

V

G

G

T

L

F

V

T

P

G

E

T

P

E

P

T

P

S

T

Q

D

V

P

E

D

A

S

I

I

-

L

-

R

-

P

F

F

N

K

D

E

L

F

L

L

A

Y

S

S

P

Y

W

I

Q

G

M

V

-

P

-

K

-

G

-

D

-

E

-

P

-

I

-

L

-

K

-

P

-

S

L

L

F

F

W

A

H

Y

T

I

V

V

F

F

-

L

-

I

-

T

M

M

G

F

A

I

T

N

A

V

F

S

I

I

V

L

L

I

R

R

G

G

V

I

Q

S

N

K

G

G

L

I

E

E

R
|
R

A
x
F

V

A

R

K

L
x
I

L

A

M

M

P

P

I

T

L

L

F

F

L

I

L

L

L

A

L

V

V

F

M

L

V

V

G

I

Y

R

-

V

-

F

-

L

-

L

G

E

M

T

G

P

A

N

G

G

A

T

F

A

V

A

E

D

A

G

M

L

D

N

F

F

M

L

F

W

S

T

P

P

D

D

F

F

S

E

A

K

I

L

G

K

G

D

D

P

A

G

V

V

-

W

L

I

V

A

A

A

M

V

G

G

Q

Q

A

I

F

F

F
|
F

T
|
T

L

L

S
|
S

L

L

G

G

L
x
F

G

G

A

A

I

I

M

I

A

T

Y

Y

G

A

S

S

Y

Y

L

V

S

R

K

K

R

D

S

Q

S

D

I

I

P

V

G

-

N

L

S

S

G

G

L

L

I

T

A

A

G

A

-

T

-

L

-
x
N

-

E

-

K

A

A

D

E

T

V

V

I

-

L

-

G

-

G

-

S

I

I

A

S

L

I

L

P

A

A

G

A

L

V

A

A

I

F

F

F

P

G

I

V

V

A

L

N

A

A

A

V

G

A

L

I

D

A

P

K

D

A

S

G

G

A

P
x
F

G

N

L

L

V

G

F

F

V

I

T

T

L

L

S

P

Y

A

G

I

F

F

G

S

Q

Q

M

T

P

A

L

G

G

G

A

T

L

F

F

L

G

G

T

F

L

L

F

W

F

F

L

F

L

L

L

L

S

F

F
|
F

A
|
A

A

G

L

L

T
|
T

S
|
S

A

S

I

I

S

A

I

I

M

M

E

Q

P

P

A

M

T

I

A

A

Y

F

L

L

V

E

E

D

T

E

R

L

G

K

W

L

K

S

R

R

P

K

K

H

A

A

-

V

-

L

-

W

-

T

A

A

A

A

A

I

V

V

A

F

A

F

A

S

A

A

W

H

L

L

V

V

G

M

I

F

-

L

-

N

G

K

S

S

L
|
L

L
x
D

A

E

F

M

N

D

V

F

W

W

A

A

G

G

binding 3-(3-chloro-5h-dibenzo[b,f]azepin-5-yl)-n,n-dimethylpropan-1-amine: R28 (= R34), Q32 (≠ V38), R189 (= R178), F190 (≠ A179), I193 (≠ L182), F316 (≠ P292), F346 (= F322), L396 (= L366), D397 (≠ L367)
binding leucine: A20 (= A26), G22 (= G28), G24 (= G30), Y106 (= Y112), F249 (= F233), T250 (= T234), S252 (= S236), F255 (≠ L239)
binding sodium ion: G18 (= G24), A20 (= A26), V21 (= V27), V21 (= V27), G22 (= G28), N25 (= N31), T250 (= T234), N282 (vs. gap), A347 (= A323), T350 (= T326), S351 (= S327)

3gwuA Leucine transporter leut in complex with sertraline (see paper)
33% identity, 80% coverage: 11:374/453 of query aligns to 3:402/509 of 3gwuA

query
sites
3gwuA

E

H

W

W

G

A

S

T

R

R

W

L

A

G

F

L

I

I

L

L

A

A

A

M

T

A

G

G

S

N

A
|
A

V

V

G
|
G

L

L

G
|
G

N

N

I

F

W
x
L

R
|
R

F

F

P

P

Y

V

V

Q

V

A

G

A

E

E

Y

N

G

G

G

G

G

G

A

A

F

F

I

M

L

I

I

P

Y

Y

L

I

A

I

C

A

I

F

L

L

A

L

I

V

G

G

I

I

P

P

V

L

M

M

M

W

A

I

E

E

I

W

L

A

I

M

G

G

R

R

R

Y

G

G

R

G

Q

A

S

Q

P

G

I

H

N

G

T

T

M

T

R

P

A

A

L

I

P

F

A

Y

E

L

F

L

G

W

A

R

G

N

R

R

W

F

W

A

Q

K

I

I

L

L

G

G

W

V

L

F

G

G

V

L

I

W

A

I

G

P

F

L

L

V

V

V

L

A

S

I

F

Y

Y
|
Y

S

V

V

Y

V

I

A

E

G

S

W

W

S

T

L

L

A

G

Y

F

V

A

P

I

Y

K

T

F

A

L

A

V

G

G

T

L

F

V

T

P

G

E

T

P

E

P

T

P

S

T

Q

D

V

P

E

D

A

S

I

I

-

L

-

R

-

P

F

F

N

K

D

E

L

F

L

L

A

Y

S

S

P

Y

W

I

Q

G

M

V

-

P

-

K

-

G

-

D

-

E

-

P

-

I

-

L

-

K

-

P

-

S

L

L

F

F

W

A

H

Y

T

I

V

V

F

F

-

L

-

I

-

T

M

M

G

F

A

I

T

N

A

V

F

S

I

I

V

L

L

I

R

R

G

G

V

I

Q

S

N

K

G

G

L

I

E

E

R

R

A

F

V

A

R

K

L

I

L

A

M

M

P

P

I

T

L

L

F

F

L

I

L

L

L

A

L

V

V

F

M

L

V

V

G

I

Y

R

-

V

-

F

-

L

-

L

G

E

M

T

G

P

A

N

G

G

A

T

F

A

V

A

E

D

A

G

M

L

D

N

F

F

M

L

F

W

S

T

P

P

D

D

F

F

S

E

A

K

I

L

G

K

G

D

D

P

A

G

V

V

-

W

L

I

V

A

A

A

M

V

G

G

Q

Q

A

I

F

F

F
|
F

T
|
T

L

L

S
|
S

L

L

G

G

L
x
F

G

G

A

A

I

I

M

I

A

T

Y

Y

G

A

S

S

Y

Y

L

V

S

R

K

K

R

D

S

Q

S

D

I

I

P

V

G

-

N

L

S

S

G

G

L

L

I

T

A

A

G

A

-

T

-

L

-

N

-

E

-

K

A

A

D

E

T

V

V

I

-

L

-

G

-

G

-

S

I

I

A

S

L

I

L

P

A

A

G

A

L

V

A

A

I

F

F

F

P

G

I

V

V

A

L

N

A

A

A

V

G

A

L

I

D

A

P

K

D

A

S

G

G
x
A

P

F

G

N

L

L

V

G

F

F

V

I

T

T

L

L

S

P

Y

A

G

I

F

F

G

S

Q

Q

M

T

P

A

L

G

G

G

A

T

L

F

F

L

G

G

T

F

L

L

F

W

F

F

L

F

L

L

L

L

S

F

F

F

A

A

A

G

L

L

T

T

S

S

A

S

I

I

S

A

I

I

M

M

E

Q

P

P

A

M

T

I

A

A

Y

F

L

L

V

E

E

D

T

E

R

L

G

K

W

L

K

S

R

R

P

K

K

H

A

A

-

V

-

L

-

W

-

T

A

A

A

A

A

I

V

V

A

F

A

F

A

S

A

A

W

H

L

L

V

V

G

M

I

F

-

L

-

N

G

K

S

S

L

L

L
x
D

A

E

F

M

N
x
D

V

F

W

W

A

A

G

G

binding leucine: A18 (= A26), G20 (= G28), G22 (= G30), Y104 (= Y112), F247 (= F233), T248 (= T234), S250 (= S236), F253 (≠ L239)
binding (1S,4S)-4-(3,4-dichlorophenyl)-N-methyl-1,2,3,4-tetrahydronaphthalen-1-amine: L25 (≠ W33), R26 (= R34), Y104 (= Y112), F247 (= F233), A313 (≠ G291), D395 (≠ L367), D398 (≠ N370)

3f4jA Crystal structure of leut bound to glycine and sodium (see paper)
33% identity, 80% coverage: 11:374/453 of query aligns to 3:402/509 of 3f4jA

query
sites
3f4jA

E

H

W

W

G

A

S

T

R

R

W

L

A

G

F

L

I

I

L

L

A

A

A

M

T

A

G
|
G

S

N

A
|
A

V
|
V

G
|
G

L

L

G
|
G

N
|
N

I

F

W

L

R

R

F

F

P

P

Y

V

V

Q

V

A

G

A

E

E

Y

N

G

G

G

G

G

G

A

A

F

F

I

M

L

I

I

P

Y

Y

L

I

A

I

C

A

I

F

L

L

A

L

I

V

G

G

I

I

P

P

V

L

M

M

M

W

A

I

E

E

I

W

L

A

I

M

G

G

R

R

R

Y

G

G

R

G

Q

A

S

Q

P

G

I

H

N

G

T

T

M

T

R

P

A

A

L

I

P

F

A

Y

E

L

F

L

G

W

A

R

G

N

R

R

W

F

W

A

Q

K

I

I

L

L

G

G

W

V

L

F

G

G

V

L

I

W

A

I

G

P

F

L

L

V

V

V

L

A

S

I

F

Y

Y
|
Y

S

V

V

Y

V

I

A

E

G

S

W

W

S

T

L

L

A

G

Y

F

V

A

P

I

Y

K

T

F

A

L

A

V

G

G

T

L

F

V

T

P

G

E

T

P

E

P

T

P

S

T

Q

D

V

P

E

D

A

S

I

I

-

L

-

R

-

P

F

F

N

K

D

E

L

F

L

L

A

Y

S

S

P

Y

W

I

Q

G

M

V

-

P

-

K

-

G

-

D

-

E

-

P

-

I

-

L

-

K

-

P

-

S

L

L

F

F

W

A

H

Y

T

I

V

V

F

F

-

L

-

I

-

T

M

M

G

F

A

I

T

N

A

V

F

S

I

I

V

L

L

I

R

R

G

G

V

I

Q

S

N

K

G

G

L

I

E

E

R

R

A

F

V

A

R

K

L

I

L

A

M

M

P

P

I

T

L

L

F

F

L

I

L

L

L

A

L

V

V

F

M

L

V

V

G

I

Y

R

-

V

-

F

-

L

-

L

G

E

M

T

G

P

A

N

G

G

A

T

F

A

V

A

E

D

A

G

M

L

D

N

F

F

M

L

F

W

S

T

P

P

D

D

F

F

S

E

A

K

I

L

G

K

G

D

D

P

A

G

V

V

-

W

L

I

V

A

A

A

M

V

G

G

Q

Q

A

I

F

F

F
|
F

T
|
T

L

L

S
|
S

L

L

G

G

L

F

G

G

A

A

I

I

M

I

A

T

Y

Y

G

A

S

S

Y

Y

L

V

S

R

K

K

R

D

S

Q

S

D

I

I

P

V

G

-

N

L

S

S

G

G

L

L

I

T

A

A

G

A

-

T

-

L

-
x
N

-

E

-

K

A

A

D

E

T

V

V

I

-

L

-

G

-

G

-

S

I

I

A

S

L

I

L

P

A

A

G

A

L

V

A

A

I

F

F

F

P

G

I

V

V

A

L

N

A

A

A

V

G

A

L

I

D

A

P

K

D

A

S

G

G

A

P

F

G

N

L

L

V

G

F

F

V

I

T

T

L

L

S

P

Y

A

G

I

F

F

G

S

Q

Q

M

T

P

A

L

G

G

G

A

T

L

F

F

L

G

G

T

F

L

L

F

W

F

F

L

F

L

L

L

L

S

F

F

F

A
|
A

A
x
G

L

L

T
|
T

S
|
S

A

S

I

I

S

A

I

I

M

M

E

Q

P

P

A

M

T

I

A

A

Y

F

L

L

V

E

E

D

T

E

R

L

G

K

W

L

K

S

R

R

P

K

K

H

A

A

-

V

-

L

-

W

-

T

A

A

A

A

A

I

V

V

A

F

A

F

A

S

A

A

W

H

L

L

V

V

G

M

I

F

-

L

-

N

G

K

S

S

L

L

L

D

A

E

F

M

N

D

V

F

W

W

A

A

G

G

binding glycine: A18 (= A26), G20 (= G28), G22 (= G30), Y104 (= Y112), F247 (= F233), T248 (= T234), S250 (= S236)
binding sodium ion: G16 (= G24), A18 (= A26), V19 (= V27), V19 (= V27), G20 (= G28), G22 (= G30), N23 (= N31), T248 (= T234), N280 (vs. gap), A345 (= A323), G346 (≠ A324), T348 (= T326), S349 (= S327)

3f3dA Crystal structure of leut bound to l-methionine and sodium (see paper)
33% identity, 80% coverage: 11:374/453 of query aligns to 3:402/509 of 3f3dA

query
sites
3f3dA

E

H

W

W

G

A

S

T

R

R

W

L

A

G

F

L

I

I

L

L

A

A

A

M

T

A

G
|
G

S
x
N

A
|
A

V
|
V

G
|
G

L

L

G
|
G

N
|
N

I

F

W

L

R

R

F

F

P

P

Y

V

V

Q

V

A

G

A

E

E

Y

N

G

G

G

G

G

G

A

A

F

F

I

M

L

I

I

P

Y

Y

L

I

A

I

C

A

I

F

L

L

A

L

I

V

G

G

I

I

P

P

V

L

M

M

M

W

A

I

E

E

I

W

L

A

I

M

G

G

R

R

R

Y

G

G

R

G

Q

A

S

Q

P

G

I

H

N

G

T

T

M

T

R

P

A

A

L

I

P

F

A

Y

E

L

F

L

G

W

A

R

G

N

R

R

W

F

W

A

Q

K

I

I

L

L

G

G

W

V

L

F

G

G

V

L

I

W

A

I

G

P

F

L

L

V

V

V

L

A

S

I

F

Y

Y
|
Y

S

V

V

Y

V

I

A

E

G

S

W

W

S

T

L

L

A

G

Y

F

V

A

P

I

Y

K

T

F

A

L

A

V

G

G

T

L

F

V

T

P

G

E

T

P

E

P

T

P

S

T

Q

D

V

P

E

D

A

S

I

I

-

L

-

R

-

P

F

F

N

K

D

E

L

F

L

L

A

Y

S

S

P

Y

W

I

Q

G

M

V

-

P

-

K

-

G

-

D

-

E

-

P

-

I

-

L

-

K

-

P

-

S

L

L

F

F

W

A

H

Y

T

I

V

V

F

F

-

L

-

I

-

T

M

M

G

F

A

I

T

N

A

V

F

S

I

I

V

L

L

I

R

R

G

G

V

I

Q

S

N

K

G

G

L

I

E

E

R

R

A

F

V

A

R

K

L

I

L

A

M

M

P

P

I

T

L

L

F

F

L

I

L

L

L

A

L

V

V

F

M

L

V

V

G

I

Y

R

-

V

-

F

-

L

-

L

G

E

M

T

G

P

A

N

G

G

A

T

F

A

V

A

E

D

A

G

M

L

D

N

F

F

M

L

F

W

S

T

P

P

D

D

F

F

S

E

A

K

I

L

G

K

G

D

D

P

A

G

V

V

-

W

L

I

V

A

A

A

M

V

G

G

Q

Q

A

I

F

F

F
|
F

T
|
T

L

L

S
|
S

L

L

G

G

L

F

G

G

A

A

I

I

M

I

A

T

Y

Y

G

A

S

S

Y

Y

L

V

S

R

K

K

R

D

S

Q

S

D

I

I

P

V

G

-

N

L

S

S

G

G

L

L

I

T

A

A

G

A

-

T

-

L

-
x
N

-

E

-

K

A

A

D

E

T

V

V

I

-

L

-

G

-

G

-

S

I

I

A

S

L

I

L

P

A

A

G

A

L

V

A

A

I

F

F

F

P

G

I

V

V

A

L

N

A

A

A

V

G

A

L

I

D

A

P

K

D

A

S

G

G

A

P

F

G

N

L

L

V

G

F

F

V

I

T

T

L

L

S

P

Y

A

G

I

F

F

G

S

Q

Q

M

T

P

A

L

G

G

G

A

T

L

F

F

L

G

G

T

F

L

L

F

W

F

F

L

F

L

L

L

L

S

F

F

F

A
|
A

A

G

L

L

T
|
T

S
|
S

A

S

I

I

S

A

I
|
I

M

M

E

Q

P

P

A

M

T

I

A

A

Y

F

L

L

V

E

E

D

T

E

R

L

G

K

W

L

K

S

R

R

P

K

K

H

A

A

-

V

-

L

-

W

-

T

A

A

A

A

A

I

V

V

A

F

A

F

A

S

A

A

W

H

L

L

V

V

G

M

I

F

-

L

-

N

G

K

S

S

L

L

L

D

A

E

F

M

N

D

V

F

W

W

A

A

G

G

binding methionine: N17 (≠ S25), A18 (= A26), G20 (= G28), G22 (= G30), Y104 (= Y112), F247 (= F233), T248 (= T234), S250 (= S236), S349 (= S327), I353 (= I331)
binding sodium ion: G16 (= G24), A18 (= A26), V19 (= V27), V19 (= V27), G20 (= G28), N23 (= N31), T248 (= T234), N280 (vs. gap), A345 (= A323), T348 (= T326), S349 (= S327)

3f3cA Crystal structure of leut bound to 4-fluoro-l-phenylalanine and sodium (see paper)
33% identity, 80% coverage: 11:374/453 of query aligns to 3:402/509 of 3f3cA

query
sites
3f3cA

E

H

W

W

G

A

S

T

R

R

W

L

A

G

F

L

I

I

L

L

A

A

A

M

T

A

G
|
G

S
x
N

A
|
A

V
|
V

G
|
G

L

L

G
|
G

N
|
N

I

F

W

L

R

R

F

F

P

P

Y

V

V

Q

V

A

G

A

E

E

Y

N

G

G

G

G

G

G

A

A

F

F

I

M

L

I

I

P

Y

Y

L

I

A

I

C

A

I

F

L

L

A

L

I

V

G

G

I

I

P

P

V

L

M

M

M

W

A

I

E

E

I

W

L

A

I

M

G

G

R

R

R

Y

G

G

R

G

Q

A

S

Q

P

G

I

H

N

G

T

T

M

T

R

P

A

A

L

I

P

F

A

Y

E

L

F

L

G

W

A

R

G

N

R

R

W

F

W

A

Q

K

I

I

L

L

G

G

W

V

L

F

G

G

V

L

I

W

A

I

G

P

F

L

L

V

V

V

L

A

S

I

F

Y

Y
|
Y

S

V

V

Y

V

I

A

E

G

S

W

W

S

T

L

L

A

G

Y

F

V

A

P

I

Y

K

T

F

A

L

A

V

G

G

T

L

F

V

T

P

G

E

T

P

E

P

T

P

S

T

Q

D

V

P

E

D

A

S

I

I

-

L

-

R

-

P

F

F

N

K

D

E

L

F

L

L

A

Y

S

S

P

Y

W

I

Q

G

M

V

-

P

-

K

-

G

-

D

-

E

-

P

-

I

-

L

-

K

-

P

-

S

L

L

F

F

W

A

H

Y

T

I

V

V

F

F

-

L

-

I

-

T

M

M

G

F

A

I

T

N

A

V

F

S

I

I

V

L

L

I

R

R

G

G

V

I

Q

S

N

K

G

G

L

I

E

E

R

R

A

F

V

A

R

K

L

I

L

A

M

M

P

P

I

T

L

L

F

F

L

I

L

L

L

A

L

V

V

F

M

L

V

V

G

I

Y

R

-

V

-

F

-

L

-

L

G

E

M

T

G

P

A

N

G

G

A

T

F

A

V

A

E

D

A

G

M

L

D

N

F

F

M

L

F

W

S

T

P

P

D

D

F

F

S

E

A

K

I

L

G

K

G

D

D

P

A

G

V

V

-

W

L

I

V

A

A

A

M

V

G

G

Q

Q

A

I

F

F

F
|
F

T
|
T

L

L

S
|
S

L

L

G

G

L
x
F

G

G

A

A

I

I

M

I

A

T

Y

Y

G

A

S

S

Y

Y

L

V

S

R

K

K

R

D

S

Q

S

D

I

I

P

V

G

-

N

L

S

S

G

G

L

L

I

T

A

A

G

A

-

T

-

L

-
x
N

-

E

-

K

A

A

D

E

T

V

V

I

-

L

-

G

-

G

-

S

I

I

A

S

L

I

L

P

A

A

G

A

L

V

A

A

I

F

F

F

P

G

I

V

V

A

L

N

A

A

A

V

G

A

L

I

D

A

P

K

D

A

S

G

G

A

P

F

G

N

L

L

V

G

F

F

V

I

T

T

L

L

S

P

Y

A

G

I

F

F

G

S

Q

Q

M

T

P

A

L

G

G

G

A

T

L

F

F

L

G

G

T

F

L

L

F

W

F

F

L

F

L

L

L

L

S

F

F

F

A
|
A

A

G

L

L

T
|
T

S
|
S

A

S

I

I

S

A

I
|
I

M

M

E

Q

P

P

A

M

T

I

A

A

Y

F

L

L

V

E

E

D

T

E

R

L

G

K

W

L

K

S

R

R

P

K

K

H

A

A

-

V

-

L

-

W

-

T

A

A

A

A

A

I

V

V

A

F

A

F

A

S

A

A

W

H

L

L

V

V

G

M

I

F

-

L

-

N

G

K

S

S

L

L

L

D

A

E

F

M

N

D

V

F

W

W

A

A

G

G

binding sodium ion: G16 (= G24), A18 (= A26), V19 (= V27), V19 (= V27), G20 (= G28), N23 (= N31), T248 (= T234), N280 (vs. gap), A345 (= A323), T348 (= T326), S349 (= S327)
binding 4-fluoro-l-phenylalanine: N17 (≠ S25), A18 (= A26), G20 (= G28), G22 (= G30), Y104 (= Y112), F247 (= F233), T248 (= T234), S250 (= S236), F253 (≠ L239), S349 (= S327), I353 (= I331)

3gwvA Leucine transporter leut in complex with r-fluoxetine (see paper)
33% identity, 80% coverage: 11:374/453 of query aligns to 3:402/498 of 3gwvA

query
sites
3gwvA

E

H

W

W

G

A

S

T

R

R

W

L

A

G

F

L

I

I

L

L

A

A

A

M

T

A

G

G

S

N

A
|
A

V

V

G
|
G

L
|
L

G
|
G

N

N

I

F

W

L

R
|
R

F

F

P

P

Y

V

V

Q

V

A

G

A

E

E

Y

N

G

G

G

G

G

G

A

A

F

F

I

M

L

I

I

P

Y

Y

L

I

A

I

C

A

I

F

L

L

A

L

I

V

G

G

I

I

P

P

V

L

M

M

M

W

A

I

E

E

I

W

L

A

I

M

G

G

R

R

R

Y

G

G

R

G

Q

A

S

Q

P

G

I

H

N

G

T

T

M

T

R

P

A

A

L

I

P

F

A

Y

E

L

F

L

G

W

A

R

G

N

R

R

W

F

W

A

Q

K

I

I

L

L

G

G

W

V

L

F

G

G

V

L

I

W

A

I

G

P

F

L

L

V

V

V

L

A

S

I

F

Y

Y
|
Y

S

V

V

Y

V

I

A

E

G

S

W

W

S

T

L

L

A

G

Y

F

V

A

P

I

Y

K

T

F

A

L

A

V

G

G

T

L

F

V

T

P

G

E

T

P

E

P

T

P

S

T

Q

D

V

P

E

D

A

S

I

I

-

L

-

R

-

P

F

F

N

K

D

E

L

F

L

L

A

Y

S

S

P

Y

W

I

Q

G

M

V

-

P

-

K

-

G

-

D

-

E

-

P

-

I

-

L

-

K

-

P

-

S

L

L

F

F

W

A

H

Y

T

I

V

V

F

F

-

L

-

I

-

T

M

M

G

F

A

I

T

N

A

V

F

S

I

I

V

L

L

I

R

R

G

G

V

I

Q

S

N

K

G

G

L

I

E

E

R

R

A

F

V

A

R

K

L

I

L

A

M

M

P

P

I

T

L

L

F

F

L

I

L

L

L

A

L

V

V

F

M

L

V

V

G

I

Y

R

-

V

-

F

-

L

-

L

G

E

M

T

G

P

A

N

G

G

A

T

F

A

V

A

E

D

A

G

M

L

D

N

F

F

M

L

F

W

S

T

P

P

D

D

F

F

S

E

A

K

I

L

G

K

G

D

D

P

A

G

V

V

-

W

L

I

V

A

A

A

M

V

G

G

Q

Q

A

I

F

F

F
|
F

T
|
T

L

L

S
|
S

L

L

G

G

L
x
F

G

G

A

A

I

I

M

I

A

T

Y

Y

G

A

S

S

Y

Y

L

V

S

R

K

K

R

D

S

Q

S

D

I

I

P

V

G

-

N

L

S

S

G

G

L

L

I

T

A

A

G

A

-

T

-

L

-

N

-

E

-

K

A

A

D

E

T

V

V

I

-

L

-

G

-

G

-

S

I

I

A

S

L

I

L

P

A

A

G

A

L

V

A

A

I

F

F

F

P

G

I

V

V

A

L

N

A

A

A

V

G

A

L

I

D

A

P

K

D

A

S

G

G
x
A

P
x
F

G

N

L

L

V

G

F

F

V

I

T

T

L

L

S

P

Y

A

G

I

F

F

G

S

Q

Q

M

T

P

A

L

G

G

G

A

T

L

F

F

L

G

G

T

F

L

L

F

W

F

F

L

F

L

L

L

L

S

F

F

F

A

A

A

G

L

L

T

T

S

S

A

S

I

I

S

A

I

I

M

M

E

Q

P

P

A

M

T

I

A

A

Y

F

L

L

V

E

E

D

T

E

R

L

G

K

W

L

K

S

R

R

P

K

K

H

A

A

-

V

-

L

-

W

-

T

A

A

A

A

A

I

V

V

A

F

A

F

A

S

A

A

W

H

L

L

V

V

G

M

I

F

-

L

-

N

G

K

S

S

L
|
L

L
x
D

A

E

F

M

N
x
D

V

F

W

W

A

A

G

G

binding leucine: A18 (= A26), G20 (= G28), G22 (= G30), Y104 (= Y112), F247 (= F233), T248 (= T234), S250 (= S236), F253 (≠ L239)
binding (3R)-N-methyl-3-phenyl-3-[4-(trifluoromethyl)phenoxy]propan-1-amine: L21 (= L29), R26 (= R34), A313 (≠ G291), F314 (≠ P292), L394 (= L366), D395 (≠ L367), D398 (≠ N370)

2qeiA Crystal structure analysis of leut complexed with l-alanine, sodium, and clomipramine (see paper)
33% identity, 80% coverage: 11:374/453 of query aligns to 4:403/511 of 2qeiA

query
sites
2qeiA

E

H

W

W

G

A

S

T

R

R

W

L

A

G

F

L

I

I

L

L

A

A

A

M

T

A

G
|
G

S

N

A
|
A

V
|
V

G
|
G

L

L

G
|
G

N
|
N

I

F

W

L

R
|
R

F

F

P

P

Y
x
V

V
x
Q

V

A

G

A

E

E

Y

N

G

G

G

G

G

G

A

A

F

F

I

M

L

I

I

P

Y

Y

L

I

A

I

C

A

I

F

L

L

A

L

I

V

G

G

I

I

P

P

V

L

M

M

M

W

A

I

E

E

I

W

L

A

I

M

G

G

R

R

R

Y

G

G

R

G

Q

A

S

Q

P

G

I

H

N

G

T

T

M

T

R

P

A

A

L

I

P

F

A

Y

E

L

F

L

G

W

A

R

G

N

R

R

W

F

W

A

Q

K

I

I

L

L

G

G

W

V

L

F

G

G

V

L

I

W

A

I

G

P

F

L

L

V

V

V

L

A

S

I

F
x
Y

Y
|
Y

S

V

V

Y

V

I

A

E

G

S

W

W

S

T

L

L

A

G

Y

F

V

A

P

I

Y

K

T

F

A

L

A

V

G

G

T

L

F

V

T

P

G

E

T

P

E

P

T

P

S

T

Q

D

V

P

E

D

A

S

I

I

-

L

-

R

-

P

F

F

N

K

D

E

L

F

L

L

A

Y

S

S

P

Y

W

I

Q

G

M

V

-

P

-

K

-

G

-

D

-

E

-

P

-

I

-

L

-

K

-

P

-

S

L

L

F

F

W

A

H

Y

T

I

V

V

F

F

-

L

-

I

-

T

M

M

G

F

A

I

T

N

A

V

F

S

I

I

V

L

L

I

R
|
R

G

G

V

I

Q

S

N

K

G

G

L

I

E

E

R
|
R

A
x
F

V

A

R

K

L

I

L

A

M

M

P

P

I

T

L

L

F

F

L

I

L

L

L

A

L

V

V

F

M

L

V

V

G

I

Y

R

-

V

-

F

-

L

-

L

G

E

M

T

G

P

A

N

G

G

A

T

F

A

V

A

E

D

A

G

M

L

D

N

F

F

M

L

F

W

S

T

P

P

D

D

F

F

S

E

A

K

I

L

G

K

G

D

D

P

A

G

V

V

-

W

L

I

V

A

A

A

M

V

G

G

Q

Q

A

I

F

F

F
|
F

T
|
T

L

L

S
|
S

L

L

G

G

L

F

G

G

A

A

I

I

M

I

A

T

Y

Y

G

A

S

S

Y

Y

L

V

S

R

K

K

R

D

S

Q

S

D

I

I

P

V

G

-

N

L

S

S

G

G

L

L

I

T

A

A

G

A

-

T

-

L

-
x
N

-

E

-

K

A

A

D

E

T

V

V

I

-

L

-

G

-

G

-

S

I

I

A

S

L

I

L

P

A

A

G

A

L

V

A

A

I

F

F

F

P

G

I

V

V

A

L

N

A

A

A

V

G

A

L

I

D

A

P

K

D

A

S

G

G

A

P
x
F

G

N

L

L

V

G

F

F

V

I

T

T

L

L

S

P

Y

A

G

I

F

F

G

S

Q

Q

M

T

P

A

L

G

G

G

A

T

L

F

F

L

G

G

T

F

L

L

F

W

F

F

L

F

L

L

L

L

S

F

F
|
F

A
|
A

A

G

L

L

T
|
T

S
|
S

A

S

I

I

S

A

I

I

M

M

E

Q

P

P

A

M

T

I

A

A

Y

F

L

L

V

E

E

D

T

E

R

L

G

K

W

L

K

S

R

R

P

K

K

H

A

A

-

V

-

L

-

W

-

T

A

A

A

A

A

I

V

V

A

F

A

F

A

S

A

A

W

H

L

L

V

V

G

M

I

F

-

L

-

N

G

K

S

S

L

L

L
x
D

A

E

F

M

N
x
D

V

F

W

W

A

A

G

G

binding alanine: A19 (= A26), G21 (= G28), G23 (= G30), Y105 (= Y112), F248 (= F233), T249 (= T234), S251 (= S236)
binding 3-(3-chloro-5h-dibenzo[b,f]azepin-5-yl)-n,n-dimethylpropan-1-amine: R27 (= R34), V30 (≠ Y37), Q31 (≠ V38), Y104 (≠ F111), R180 (= R170), R188 (= R178), F189 (≠ A179), F315 (≠ P292), F345 (= F322), D396 (≠ L367), D399 (≠ N370)
binding sodium ion: G17 (= G24), A19 (= A26), V20 (= V27), V20 (= V27), G21 (= G28), N24 (= N31), T249 (= T234), N281 (vs. gap), A346 (= A323), T349 (= T326), S350 (= S327)

2qb4A Crystal structure analysis of leut complexed with l-leucine, sodium and desipramine (see paper)
33% identity, 80% coverage: 11:374/453 of query aligns to 3:402/510 of 2qb4A

query
sites
2qb4A

E

H

W

W

G

A

S

T

R

R

W

L

A

G

F

L

I

I

L

L

A

A

A

M

T

A

G
|
G

S

N

A
|
A

V
|
V

G
|
G

L
|
L

G
|
G

N
|
N

I

F

W

L

R
|
R

F

F

P

P

Y
x
V

V
x
Q

V

A

G

A

E

E

Y

N

G

G

G

G

G

G

A

A

F

F

I

M

L

I

I

P

Y

Y

L

I

A

I

C

A

I

F

L

L

A

L

I

V

G

G

I

I

P

P

V

L

M

M

M

W

A

I

E

E

I

W

L

A

I

M

G

G

R

R

R

Y

G

G

R

G

Q

A

S

Q

P

G

I

H

N

G

T

T

M

T

R

P

A

A

L

I

P

F

A

Y

E

L

F

L

G

W

A

R

G

N

R

R

W

F

W

A

Q

K

I

I

L

L

G

G

W

V

L

F

G

G

V

L

I

W

A

I

G

P

F

L

L

V

V

V

L

A

S

I

F

Y

Y
|
Y

S

V

V

Y

V
x
I

A

E

G

S

W

W

S

T

L

L

A

G

Y

F

V

A

P

I

Y

K

T

F

A

L

A

V

G

G

T

L

F

V

T

P

G

E

T

P

E

P

T

P

S

T

Q

D

V

P

E

D

A

S

I

I

-

L

-

R

-

P

F

F

N

K

D

E

L

F

L

L

A

Y

S

S

P

Y

W

I

Q

G

M

V

-

P

-

K

-

G

-

D

-

E

-

P

-

I

-

L

-

K

-

P

-

S

L

L

F

F

W

A

H

Y

T

I

V

V

F

F

-

L

-

I

-

T

M

M

G

F

A

I

T

N

A

V

F

S

I

I

V

L

L

I

R

R

G

G

V

I

Q

S

N

K

G

G

L

I

E

E

R
|
R

A
x
F

V

A

R

K

L
x
I

L

A

M

M

P

P

I

T

L

L

F

F

L

I

L

L

L

A

L

V

V

F

M

L

V

V

G

I

Y

R

-

V

-

F

-

L

-

L

G

E

M

T

G

P

A

N

G

G

A

T

F

A

V

A

E

D

A

G

M

L

D

N

F

F

M

L

F

W

S

T

P

P

D

D

F

F

S

E

A

K

I

L

G

K

G

D

D

P

A

G

V

V

-

W

L

I

V

A

A

A

M

V

G

G

Q

Q

A

I

F

F

F
|
F

T
|
T

L

L

S
|
S

L

L

G

G

L
x
F

G

G

A

A

I

I

M

I

A

T

Y

Y

G

A

S

S

Y

Y

L

V

S

R

K

K

R

D

S

Q

S

D

I

I

P

V

G

-

N

L

S

S

G

G

L

L

I

T

A

A

G

A

-

T

-

L

-
x
N

-

E

-

K

A

A

D

E

T

V

V

I

-

L

-

G

-

G

-

S

I

I

A

S

L

I

L

P

A

A

G

A

L

V

A

A

I

F

F

F

P

G

I

V

V

A

L

N

A

A

A

V

G

A

L

I

D

A

P

K

D

A

S

G

G
x
A

P
x
F

G

N

L

L

V

G

F

F

V

I

T

T

L

L

S

P

Y

A

G

I

F

F

G

S

Q

Q

M

T

P

A

L

G

G

G

A

T

L

F

F

L

G

G

T

F

L

L

F

W

F

F

L

F

L

L

L

L

S

F

F
|
F

A
|
A

A

G

L

L

T
|
T

S
|
S

A

S

I

I

S

A

I

I

M

M

E

Q

P

P

A

M

T

I

A

A

Y

F

L

L

V

E

E

D

T

E

R

L

G

K

W

L

K

S

R

R

P

K

K

H

A

A

-

V

-

L

-

W

-

T

A

A

A

A

A

I

V

V

A

F

A

F

A

S

A

A

W

H

L

L

V

V

G

M

I

F

-

L

-

N

G

K

S

S

L

L

L
x
D

A

E

F

M

N

D

V

F

W

W

A

A

G

G

binding 3-(10,11-dihydro-5h-dibenzo[b,f]azepin-5-yl)-n-methylpropan-1-amine: R26 (= R34), V29 (≠ Y37), Q30 (≠ V38), I107 (≠ V115), R187 (= R178), F188 (≠ A179), I191 (≠ L182), A313 (≠ G291), F314 (≠ P292), F344 (= F322), D395 (≠ L367)
binding leucine: A18 (= A26), G20 (= G28), L21 (= L29), G22 (= G30), Y104 (= Y112), F247 (= F233), T248 (= T234), S250 (= S236), F253 (≠ L239)
binding sodium ion: G16 (= G24), A18 (= A26), V19 (= V27), V19 (= V27), G20 (= G28), N23 (= N31), T248 (= T234), N280 (vs. gap), A345 (= A323), T348 (= T326), S349 (= S327)

2q72A Crystal structure analysis of leut complexed with l-leucine, sodium, and imipramine (see paper)
33% identity, 80% coverage: 11:374/453 of query aligns to 4:403/511 of 2q72A

query
sites
2q72A

E

H

W

W

G

A

S

T

R

R

W

L

A

G

F

L

I

I

L

L

A

A

A

M

T

A

G
|
G

S

N

A
|
A

V
|
V

G
|
G

L
|
L

G
|
G

N
|
N

I

F

W

L

R
|
R

F

F

P

P

Y
x
V

V
x
Q

V

A

G

A

E

E

Y

N

G

G

G

G

G

G

A

A

F

F

I

M

L

I

I

P

Y

Y

L

I

A

I

C

A

I

F

L

L

A

L

I

V

G

G

I

I

P

P

V

L

M

M

M

W

A

I

E

E

I

W

L

A

I

M

G

G

R

R

R

Y

G

G

R

G

Q

A

S

Q

P

G

I

H

N

G

T

T

M

T

R

P

A

A

L

I

P

F

A

Y

E

L

F

L

G

W

A

R

G

N

R

R

W

F

W

A

Q

K

I

I

L

L

G

G

W

V

L

F

G

G

V

L

I

W

A

I

G

P

F

L

L

V

V

V

L

A

S

I

F

Y

Y
|
Y

S

V

V

Y

V

I

A

E

G

S

W

W

S

T

L

L

A

G

Y

F

V

A

P

I

Y

K

T

F

A

L

A

V

G

G

T

L

F

V

T

P

G

E

T

P

E

P

T

P

S

T

Q

D

V

P

E

D

A

S

I

I

-

L

-

R

-

P

F

F

N

K

D

E

L

F

L

L

A

Y

S

S

P

Y

W

I

Q

G

M

V

-

P

-

K

-

G

-

D

-

E

-

P

-

I

-

L

-

K

-

P

-

S

L

L

F

F

W

A

H

Y

T

I

V

V

F

F

-

L

-

I

-

T

M

M

G

F

A

I

T

N

A

V

F

S

I

I

V

L

L

I

R

R

G

G

V

I

Q

S

N

K

G

G

L

I

E

E

R
|
R

A
x
F

V

A

R

K

L
x
I

L

A

M

M

P

P

I

T

L

L

F

F

L

I

L

L

L

A

L

V

V

F

M

L

V

V

G

I

Y

R

-

V

-

F

-

L

-

L

G

E

M

T

G

P

A

N

G

G

A

T

F

A

V

A

E

D

A

G

M

L

D

N

F

F

M

L

F

W

S

T

P

P

D

D

F

F

S

E

A

K

I

L

G

K

G

D

D

P

A

G

V

V

-

W

L

I

V

A

A

A

M

V

G

G

Q

Q

A

I

F

F

F
|
F

T
|
T

L

L

S
|
S

L

L

G

G

L
x
F

G

G

A

A

I

I

M

I

A

T

Y

Y

G

A

S

S

Y

Y

L

V

S

R

K

K

R

D

S

Q

S

D

I

I

P

V

G

-

N

L

S

S

G

G

L

L

I

T

A

A

G

A

-

T

-

L

-
x
N

-

E

-

K

A

A

D

E

T

V

V

I

-

L

-

G

-

G

-

S

I

I

A

S

L

I

L

P

A

A

G

A

L

V

A

A

I

F

F

F

P

G

I

V

V

A

L

N

A

A

A

V

G

A

L

I

D

A

P

K

D

A

S

G

G
x
A

P
x
F

G

N

L

L

V

G

F

F

V

I

T

T

L

L

S

P

Y

A

G

I

F

F

G

S

Q

Q

M

T

P

A

L

G

G

G

A

T

L

F

F

L

G

G

T

F

L

L

F

W

F

F

L

F

L

L

L

L

S

F

F
|
F

A
|
A

A

G

L

L

T
|
T

S
|
S

A

S

I

I

S

A

I

I

M

M

E

Q

P

P

A

M

T

I

A

A

Y

F

L

L

V

E

E

D

T

E

R

L

G

K

W

L

K

S

R

R

P

K

K

H

A

A

-

V

-

L

-

W

-

T

A

A

A

A

A

I

V

V

A

F

A

F

A

S

A

A

W

H

L

L

V

V

G

M

I

F

-

L

-

N

G

K

S

S

L

L

L
x
D

A

E

F

M

N

D

V

F

W

W

A

A

G

G

binding 3-(5h-dibenzo[b,f]azepin-5-yl)-n,n-dimethylpropan-1-amine: R27 (= R34), V30 (≠ Y37), Q31 (≠ V38), R188 (= R178), F189 (≠ A179), I192 (≠ L182), A314 (≠ G291), F315 (≠ P292), F345 (= F322), D396 (≠ L367)
binding leucine: A19 (= A26), G21 (= G28), L22 (= L29), G23 (= G30), Y105 (= Y112), F248 (= F233), T249 (= T234), S251 (= S236), F254 (≠ L239)
binding sodium ion: G17 (= G24), A19 (= A26), V20 (= V27), V20 (= V27), G21 (= G28), N24 (= N31), T249 (= T234), N281 (vs. gap), A346 (= A323), T349 (= T326), S350 (= S327)

3uspA Crystal structure of leut in heptyl-beta-d-selenoglucoside (see paper)
33% identity, 80% coverage: 11:374/453 of query aligns to 3:402/503 of 3uspA

query
sites
3uspA

E

H

W

W

G

A

S

T

R

R

W

L

A

G

F

L

I

I

L

L

A

A

A

M

T

A

G
|
G

S

N

A
|
A

V
|
V

G
|
G

L

L

G
|
G

N
|
N

I

F

W

L

R

R

F

F

P

P

Y

V

V

Q

V

A

G

A

E

E

Y

N

G

G

G

G

G

G

A

A

F

F

I

M

L

I

I

P

Y

Y

L

I

A

I

C

A

I

F

L

L

A

L

I

V

G

G

I

I

P

P

V

L

M

M

M

W

A

I

E

E

I

W

L

A

I

M

G

G

R

R

R

Y

G

G

R

G

Q

A

S

Q

P

G

I

H

N

G

T

T

M

T

R

P

A

A

L

I

P

F

A

Y

E

L

F

L

G

W

A

R

G

N

R

R

W

F

W

A

Q

K

I

I

L

L

G

G

W

V

L

F

G

G

V

L

I

W

A

I

G

P

F

L

L

V

V

V

L

A

S

I

F

Y

Y

Y

S

V

V

Y

V

I

A

E

G

S

W

W

S

T

L

L

A

G

Y

F

V

A

P

I

Y
x
K

T

F

A

L

A

V

G

G

T
x
L

F

V

T

P

G

E

T

P

E

P

T

P

S

T

Q

D

V

P

E

D

A

S

I

I

-

L

-

R

-

P

F

F

N

K

D

E

L

F

L

L

A

Y

S

S

P

Y

W

I

Q

G

M

V

-

P

-

K

-

G

-

D

-
x
E

-

P

-
x
I

-

L

-
x
K

-
x
P

-

S

L

L

F
|
F

W

A

H
x
Y

T

I

V

V

F

F

-

L

-

I

-

T

M

M

G

F

A

I

T

N

A

V

F

S

I

I

V

L

L

I

R

R

G

G

V

I

Q

S

N

K

G

G

L

I

E

E

R

R

A

F

V

A

R

K

L

I

L

A

M

M

P

P

I

T

L

L

F

F

L

I

L

L

L

A

L

V

V

F

M

L

V

V

G

I

Y

R

-

V

-

F

-

L

-

L

G

E

M

T

G

P

A

N

G

G

A

T

F

A

V

A

E

D

A

G

M

L

D

N

F

F

M

L

F

W

S

T

P

P

D

D

F

F

S

E

A

K

I

L

G

K

G

D

D

P

A

G

V

V

-

W

L

I

V

A

A

A

M

V

G

G

Q

Q

A

I

F

F

F
|
F

T
|
T

L

L

S
|
S

L

L

G

G

L
x
F

G

G

A

A

I

I

M

I

A

T

Y

Y

G

A

S

S

Y

Y

L

V

S

R

K

K

R

D

S

Q

S

D

I

I

P

V

G

-

N

L

S

S

G

G

L

L

I

T

A

A

G

A

-

T

-

L

-
x
N

-

E

-

K

A

A

D

E

T

V

V

I

-

L

-

G

-

G

-

S

I

I

A

S

L

I

L

P

A

A

G

A

L

V

A

A

I

F

F

F

P

G

I

V

V

A

L

N

A

A

A

V

G

A

L

I

D

A

P

K

D

A

S

G

G

A

P

F

G

N

L

L

V

G

F

F

V

I

T

T

L

L

S

P

Y

A

G

I

F

F

G

S

Q

Q

M

T

P

A

L

G

G

G

A

T

L

F

F

L

G

G

T

F

L

L

F

W

F

F

L

F

L

L

L

L

S

F

F

F

A
|
A

A

G

L

L

T
|
T

S
|
S

A

S

I

I

S

A

I

I

M

M

E

Q

P

P

A

M

T

I

A

A

Y

F

L

L

V

E

E

D

T

E

R

L

G

K

W

L

K

S

R

R

P

K

K

H

A

A

-

V

-

L

-

W

-

T

A

A

A

A

A

I

V

V

A

F

A

F

A

S

A

A

W
x
H

L
|
L

V

V

G

M

I
x
F

-

L

-

N

G

K

S

S

L
|
L

L
x
D

A

E

F

M

N
x
D

V

F

W

W

A

A

G

G

binding heptyl 1-seleno-beta-D-glucopyranoside: K117 (≠ Y125), L122 (≠ T130), E153 (vs. gap), I155 (vs. gap), K157 (vs. gap), P158 (vs. gap), F161 (= F155), Y163 (≠ H157), H385 (≠ W359), H385 (≠ W359), L386 (= L360), F389 (≠ I363), F389 (≠ I363), L394 (= L366), D395 (≠ L367), D398 (≠ N370)
binding leucine: A18 (= A26), G20 (= G28), G22 (= G30), F247 (= F233), T248 (= T234), S250 (= S236), F253 (≠ L239)
binding sodium ion: G16 (= G24), A18 (= A26), V19 (= V27), V19 (= V27), G20 (= G28), N23 (= N31), T248 (= T234), N280 (vs. gap), A345 (= A323), T348 (= T326), S349 (= S327)

Sites not aligning to the query:

binding heptyl 1-seleno-beta-D-glucopyranoside: 439, 442

Query Sequence

>WP_011815179.1 NCBI__GCF_000015585.1:WP_011815179.1
MKDLRQSIHGEWGSRWAFILAATGSAVGLGNIWRFPYVVGEYGGGAFILIYLACILAIGI
PVMMAEILIGRRGRQSPINTMRALPAEFGAGRWWQILGWLGVIAGFLVLSFYSVVAGWSL
AYVPYTAAGTFTGTETSQVEAIFNDLLASPWQMLFWHTVFMGATAFIVLRGVQNGLERAV
RLLMPILFLLLLVMVGYGMGAGAFVEAMDFMFSPDFSAIGGDAVLVAMGQAFFTLSLGLG
AIMAYGSYLSKRSSIPGNSGLIAGADTVIALLAGLAIFPIVLAAGLDPDSGPGLVFVTLS
YGFGQMPLGALFGTLFFLLLSFAALTSAISIMEPATAYLVETRGWKRPKAAAAVAAAAWL
VGIGSLLAFNVWAGFTIAGLNFQELAEYLSTSVMLPLGGLLMALFAGWVLPRSVTTEELA
LPDWVYRGWLVLVRFVVPAAILVVFANSLGLLG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory