SitesBLAST

P39976 D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3; D-2HG--pyruvate transhydrogenase DLD3; (R)-2-hydroxyglutarate--pyruvate transhydrogenase; D-lactate dehydrogenase [cytochrome] 3; D-lactate ferricytochrome C oxidoreductase; D-LCR; EC 1.1.99.40; EC 1.1.2.4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
25% identity, 52% coverage: 15:206/368 of query aligns to 80:272/496 of P39976

query
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P39976

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Sites not aligning to the query:

17 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid (see paper)
27% identity, 39% coverage: 45:188/368 of query aligns to 85:226/455 of 8jduA

query
sites
8jduA

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binding flavin-adenine dinucleotide: L90 (= L50), P132 (= P92), G133 (≠ A96), A134 (≠ P97), G140 (= G103), M141 (= M104), A143 (= A106), T144 (≠ A107), A146 (≠ L109), S147 (= S110), E200 (≠ L160), G201 (= G161), I206 (≠ L166)

Sites not aligning to the query:

binding 2-oxopentanoic acid: 318, 322, 369, 376, 414
binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 322, 413, 450
binding manganese (ii) ion: 369, 376, 413

8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
27% identity, 39% coverage: 45:188/368 of query aligns to 85:226/456 of 8jdsA

query
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8jdsA

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binding flavin-adenine dinucleotide: L90 (= L50), P132 (= P92), G133 (≠ A96), A134 (≠ P97), G140 (= G103), M141 (= M104), A143 (= A106), T144 (≠ A107), A146 (≠ L109), S147 (= S110), E200 (≠ L160), G201 (= G161), I206 (≠ L166)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 32, 68, 70, 71, 72, 73, 74, 78, 79, 323, 414, 415, 451
binding manganese (ii) ion: 370, 377, 414
binding pyruvic acid: 319, 370, 377, 415

8jdeA Crystal structure of mldhd in complex with d-lactate (see paper)
27% identity, 39% coverage: 45:188/368 of query aligns to 85:226/455 of 8jdeA

query
sites
8jdeA

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binding flavin-adenine dinucleotide: L90 (= L50), P132 (= P92), G133 (≠ A96), A134 (≠ P97), G140 (= G103), M141 (= M104), A143 (= A106), T144 (≠ A107), A146 (≠ L109), S147 (= S110), E200 (≠ L160), G201 (= G161), I206 (≠ L166)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 322, 413, 414, 450
binding lactic acid: 318, 369, 376, 414
binding manganese (ii) ion: 369, 376, 413

8jdzA Crystal structure of mldhd in complex with 2-keto-3-methylvaleric acid (see paper)
27% identity, 39% coverage: 45:188/368 of query aligns to 85:226/454 of 8jdzA

query
sites
8jdzA

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binding flavin-adenine dinucleotide: L90 (= L50), P132 (= P92), G133 (≠ A96), A134 (≠ P97), G140 (= G103), M141 (= M104), A143 (= A106), T144 (≠ A107), A146 (≠ L109), S147 (= S110), E200 (≠ L160), G201 (= G161), I206 (≠ L166)

Sites not aligning to the query:

binding (3S)-3-methyl-2-oxopentanoic acid: 318, 322, 369, 376, 413
binding flavin-adenine dinucleotide: 32, 68, 70, 71, 72, 73, 74, 78, 79, 322, 412, 413, 449
binding manganese (ii) ion: 369, 376, 412

8jdyA Crystal structure of mldhd in complex with 2-ketoisocaproic acid (see paper)
27% identity, 39% coverage: 45:188/368 of query aligns to 85:226/454 of 8jdyA

query
sites
8jdyA

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binding flavin-adenine dinucleotide: L90 (= L50), P132 (= P92), G133 (≠ A96), A134 (≠ P97), G140 (= G103), M141 (= M104), A143 (= A106), T144 (≠ A107), A146 (≠ L109), S147 (= S110), E200 (≠ L160), G201 (= G161), I206 (≠ L166)

Sites not aligning to the query:

binding 2-oxo-4-methylpentanoic acid: 318, 322, 336, 369, 376, 413
binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 412, 449
binding manganese (ii) ion: 369, 376, 412

8jdtA Crystal structure of mldhd in complex with 2-ketobutanoic acid (see paper)
27% identity, 39% coverage: 45:188/368 of query aligns to 85:226/455 of 8jdtA

query
sites
8jdtA

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A

A

binding flavin-adenine dinucleotide: L90 (= L50), P132 (= P92), G133 (≠ A96), A134 (≠ P97), G140 (= G103), M141 (= M104), A143 (= A106), T144 (≠ A107), A146 (≠ L109), S147 (= S110), E200 (≠ L160), G201 (= G161), I206 (≠ L166)

Sites not aligning to the query:

binding 2-ketobutyric acid: 318, 369, 376, 414
binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 322, 413, 414, 450
binding manganese (ii) ion: 369, 376, 413

8jdrA Crystal structure of h405a mldhd in complex with d-2-hydroxy-3-methyl- valeric acid (see paper)
27% identity, 39% coverage: 45:188/368 of query aligns to 85:226/456 of 8jdrA

query
sites
8jdrA

G

G

D

V

L

C

L

I

D

N

L
|
L

R

T

P

H

L

M

A

D

G

Q

V

I

R

T

S

E

Y

L

E

N

P

T

T

E

E

D

L

F

V

S

V

V

T

V

V

V

R

E

A

P

G

G

E

V

P

T

L

R

A

K

R

A

L

L

E

N

A

T

L

H

L

L

A

R

D

D

R

S

G

G

Q

L

H

W

L

F

A

P

F

V

E

D

P
|
P

P

-

R

-

F

-

A
x
G

P
x
A

G

D

G

A

T

S

V

L

G

C

G
|
G

M
|
M

V

A

A
|
A

A
x
T

G

G

L
x
A

S
|
S

G

G

P

T

G

N

R

A

I

V

A

R

A

Y

G

G

A

T

L

M

R

R

D

D

H

N

V

V

L

I

G

N

V

L

R

E

L

V

N

L

G

P

R

D

G

G

E

R

C

L

L

L

S

H

F

T

G

A

G

G

-

R

-

G

-

R

Q

H

M

Y

I

R

K

K

N

S

V

A

A

A

G

G

Y

Y

D

N

V

L

S

T

R

G

L

L

M

F

A

V

G

G

S

S

L
x
E

G
|
G

I

T

L

L

G

G

V

I

L
x
I

L

T

E

S

V

T

S

T

L

L

K

R

V

L

L

H

P

P

R

A

P

P

P

E

A

A

S

-

V

-

T

T

I

V

S

A

L

A

D

T

C

C

A

A

binding flavin-adenine dinucleotide: L90 (= L50), P132 (= P92), G133 (≠ A96), A134 (≠ P97), G140 (= G103), M141 (= M104), A143 (= A106), T144 (≠ A107), A146 (≠ L109), S147 (= S110), E200 (≠ L160), G201 (= G161), I206 (≠ L166)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 324, 370, 414, 451
binding (2R,3S)-3-methyl-2-oxidanyl-pentanoic acid: 319, 323, 415

8jdqA Crystal structure of h405a mldhd in complex with d-2-hydroxyisocaproic acid (see paper)
27% identity, 39% coverage: 45:188/368 of query aligns to 85:226/456 of 8jdqA

query
sites
8jdqA

G

G

D

V

L

C

L

I

D

N

L
|
L

R

T

P

H

L

M

A

D

G

Q

V

I

R

T

S

E

Y

L

E

N

P

T

T

E

E

D

L

F

V

S

V

V

T

V

V

V

R

E

A

P

G

G

E

V

P

T

L

R

A

K

R

A

L

L

E

N

A

T

L

H

L

L

A

R

D

D

R

S

G

G

Q

L

H

W

L

F

A

P

F

V

E

D

P
|
P

P

-

R

-

F

-

A
x
G

P
x
A

G

D

G

A

T

S

V

L

G

C

G
|
G

M
|
M

V

A

A
|
A

A
x
T

G

G

L
x
A

S
|
S

G

G

P

T

G

N

R

A

I

V

A

R

A

Y

G

G

A

T

L

M

R

R

D

D

H

N

V

V

L

I

G

N

V

L

R

E

L

V

N

L

G

P

R

D

G

G

E

R

C

L

L

L

S

H

F

T

G

A

G

G

-

R

-

G

-

R

Q

H

M

Y

I

R

K

K

N

S

V

A

A

A

G

G

Y

Y

D

N

V

L

S

T

R

G

L

L

M

F

A

V

G

G

S

S

L
x
E

G
|
G

I

T

L

L

G

G

V

I

L
x
I

L

T

E

S

V

T

S

T

L

L

K

R

V

L

L

H

P

P

R

A

P

P

P

E

A

A

S

-

V

-

T

T

I

V

S

A

L

A

D

T

C

C

A

A

binding flavin-adenine dinucleotide: L90 (= L50), P132 (= P92), G133 (≠ A96), A134 (≠ P97), G140 (= G103), M141 (= M104), A143 (= A106), T144 (≠ A107), A146 (≠ L109), S147 (= S110), E200 (≠ L160), G201 (= G161), I206 (≠ L166)

Sites not aligning to the query:

binding (2R)-2-hydroxy-4-methylpentanoic acid: 319, 323, 370, 415
binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 370, 414, 451

8jdoA Crystal structure of h405a mldhd in complex with d-2-hydroxyhexanoic acid (see paper)
27% identity, 39% coverage: 45:188/368 of query aligns to 85:226/456 of 8jdoA

query
sites
8jdoA

G

G

D

V

L

C

L

I

D

N

L
|
L

R

T

P

H

L

M

A

D

G

Q

V

I

R

T

S

E

Y

L

E

N

P

T

T

E

E

D

L

F

V

S

V

V

T

V

V

V

R

E

A

P

G

G

E

V

P

T

L

R

A

K

R

A

L

L

E

N

A

T

L

H

L

L

A

R

D

D

R

S

G

G

Q

L

H

W

L

F

A

P

F

V

E

D

P
|
P

P

-

R

-

F

-

A
x
G

P
x
A

G

D

G

A

T

S

V

L

G

C

G
|
G

M
|
M

V

A

A
|
A

A
x
T

G

G

L
x
A

S
|
S

G

G

P

T

G

N

R

A

I

V

A

R

A

Y

G

G

A

T

L

M

R

R

D

D

H

N

V

V

L

I

G

N

V

L

R

E

L

V

N

L

G

P

R

D

G

G

E

R

C

L

L

L

S

H

F

T

G

A

G

G

-

R

-

G

-

R

Q

H

M

Y

I

R

K

K

N

S

V

A

A

A

G

G

Y

Y

D

N

V

L

S

T

R

G

L

L

M

F

A

V

G

G

S

S

L
x
E

G
|
G

I

T

L

L

G

G

V

I

L
x
I

L

T

E

S

V

T

S

T

L

L

K

R

V

L

L

H

P

P

R

A

P

P

P

E

A

A

S

-

V

-

T

T

I

V

S

A

L

A

D

T

C

C

A

A

binding flavin-adenine dinucleotide: L90 (= L50), P132 (= P92), G133 (≠ A96), A134 (≠ P97), G140 (= G103), M141 (= M104), A143 (= A106), T144 (≠ A107), A146 (≠ L109), S147 (= S110), E200 (≠ L160), G201 (= G161), I206 (≠ L166)

Sites not aligning to the query:

binding (2R)-2-hydroxyhexanoic acid: 319, 323, 415
binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 324, 370, 414, 451

8jdnA Crystal structure of h405a mldhd in complex with d-2-hydroxyvaleric acid (see paper)
27% identity, 39% coverage: 45:188/368 of query aligns to 85:226/456 of 8jdnA

query
sites
8jdnA

G

G

D

V

L

C

L

I

D

N

L
|
L

R

T

P

H

L

M

A

D

G

Q

V

I

R

T

S

E

Y

L

E

N

P

T

T

E

E

D

L

F

V

S

V

V

T

V

V

V

R

E

A

P

G

G

E

V

P

T

L

R

A

K

R

A

L

L

E

N

A

T

L

H

L

L

A

R

D

D

R

S

G

G

Q

L

H

W

L

F

A

P

F

V

E

D

P
|
P

P

-

R

-

F

-

A
x
G

P
x
A

G

D

G

A

T

S

V

L

G

C

G
|
G

M
|
M

V

A

A
|
A

A
x
T

G

G

L
x
A

S
|
S

G

G

P

T

G

N

R

A

I

V

A

R

A

Y

G

G

A

T

L

M

R

R

D

D

H

N

V

V

L

I

G

N

V

L

R

E

L

V

N

L

G

P

R

D

G

G

E

R

C

L

L

L

S

H

F

T

G

A

G

G

-

R

-

G

-

R

Q

H

M

Y

I

R

K

K

N

S

V

A

A

A

G

G

Y

Y

D

N

V

L

S

T

R

G

L

L

M

F

A

V

G

G

S

S

L
x
E

G
|
G

I

T

L

L

G

G

V

I

L
x
I

L

T

E

S

V

T

S

T

L

L

K

R

V

L

L

H

P

P

R

A

P

P

P

E

A

A

S

-

V

-

T

T

I

V

S

A

L

A

D

T

C

C

A

A

binding flavin-adenine dinucleotide: L90 (= L50), P132 (= P92), G133 (≠ A96), A134 (≠ P97), G140 (= G103), M141 (= M104), A143 (= A106), T144 (≠ A107), A146 (≠ L109), S147 (= S110), E200 (≠ L160), G201 (= G161), I206 (≠ L166)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 370, 414, 451
binding (2R)-2-oxidanylpentanoic acid: 319, 323, 415

8jdgA Crystal structure of h405a mldhd in complex with d-2-hydroxybutanoic acid (see paper)
27% identity, 39% coverage: 45:188/368 of query aligns to 85:226/456 of 8jdgA

query
sites
8jdgA

G

G

D

V

L

C

L

I

D

N

L
|
L

R

T

P

H

L

M

A

D

G

Q

V

I

R

T

S

E

Y

L

E

N

P

T

T

E

E

D

L

F

V

S

V

V

T

V

V

V

R

E

A

P

G

G

E

V

P

T

L

R

A

K

R

A

L

L

E

N

A

T

L

H

L

L

A

R

D

D

R

S

G

G

Q

L

H

W

L

F

A

P

F

V

E

D

P
|
P

P

-

R

-

F

-

A
x
G

P
x
A

G

D

G

A

T

S

V

L

G

C

G
|
G

M
|
M

V

A

A
|
A

A
x
T

G

G

L
x
A

S
|
S

G

G

P

T

G

N

R

A

I

V

A

R

A

Y

G

G

A

T

L

M

R

R

D

D

H

N

V

V

L

I

G

N

V

L

R

E

L

V

N

L

G

P

R

D

G

G

E

R

C

L

L

L

S

H

F

T

G

A

G

G

-

R

-

G

-

R

Q

H

M

Y

I

R

K

K

N

S

V

A

A

A

G

G

Y

Y

D

N

V

L

S

T

R

G

L

L

M

F

A

V

G

G

S

S

L
x
E

G
|
G

I

T

L

L

G

G

V

I

L
x
I

L

T

E

S

V

T

S

T

L

L

K

R

V

L

L

H

P

P

R

A

P

P

P

E

A

A

S

-

V

-

T

T

I

V

S

A

L

A

D

T

C

C

A

A

binding flavin-adenine dinucleotide: L90 (= L50), P132 (= P92), G133 (≠ A96), A134 (≠ P97), G140 (= G103), M141 (= M104), A143 (= A106), T144 (≠ A107), A146 (≠ L109), S147 (= S110), E200 (≠ L160), G201 (= G161), I206 (≠ L166)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 370, 414, 451
binding (2R)-2-oxidanylbutanoic acid: 319, 415

8jdbA Crystal structure of h405a mldhd in complex with d-2-hydroxyoctanoic acid (see paper)
27% identity, 39% coverage: 45:188/368 of query aligns to 85:226/456 of 8jdbA

query
sites
8jdbA

G

G

D

V

L

C

L

I

D

N

L
|
L

R

T

P

H

L

M

A

D

G

Q

V

I

R

T

S

E

Y

L

E

N

P

T

T

E

E

D

L

F

V

S

V

V

T

V

V

V

R

E

A

P

G

G

E

V

P

T

L

R

A

K

R

A

L

L

E

N

A

T

L

H

L

L

A

R

D

D

R

S

G

G

Q

L

H

W

L

F

A

P

F

V

E

D

P
|
P

P

-

R

-

F

-

A
x
G

P
x
A

G

D

G

A

T

S

V

L

G

C

G
|
G

M
|
M

V

A

A
|
A

A
x
T

G

G

L
x
A

S
|
S

G

G

P

T

G

N

R

A

I

V

A

R

A

Y

G

G

A

T

L

M

R

R

D

D

H

N

V

V

L

I

G

N

V

L

R

E

L

V

N

L

G

P

R

D

G

G

E

R

C

L

L

L

S

H

F

T

G

A

G

G

-

R

-

G

-

R

Q

H

M

Y

I

R

K

K

N

S

V

A

A

A

G

G

Y

Y

D

N

V

L

S

T

R

G

L

L

M

F

A

V

G

G

S

S

L
x
E

G
|
G

I

T

L

L

G

G

V

I

L
x
I

L

T

E

S

V

T

S

T

L

L

K

R

V

L

L

H

P

P

R

A

P

P

P

E

A

A

S

-

V

-

T

T

I

V

S

A

L

A

D

T

C

C

A

A

binding flavin-adenine dinucleotide: L90 (= L50), P132 (= P92), G133 (≠ A96), A134 (≠ P97), G140 (= G103), M141 (= M104), A143 (= A106), T144 (≠ A107), A146 (≠ L109), S147 (= S110), E200 (≠ L160), G201 (= G161), I206 (≠ L166)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 324, 370, 414, 451
binding (2R)-2-oxidanyloctanoic acid: 75, 319, 323, 415

8jdxA Crystal structure of mldhd in complex with 2-ketoisovaleric acid (see paper)
27% identity, 39% coverage: 45:188/368 of query aligns to 85:226/455 of 8jdxA

query
sites
8jdxA

G

G

D

V

L

C

L

I

D

N

L
|
L

R

T

P

H

L

M

A

D

G

Q

V

I

R

T

S

E

Y

L

E

N

P

T

T

E

E

D

L

F

V

S

V

V

T

V

V

V

R

E

A

P

G

G

E

V

P

T

L

R

A

K

R

A

L

L

E

N

A

T

L

H

L

L

A

R

D

D

R

S

G

G

Q

L

H

W

L

F

A

P

F

V

E

D

P
|
P

P

-

R

-

F

-

A
x
G

P
x
A

G

D

G

A

T

S

V

L

G

C

G
|
G

M
|
M

V

A

A
|
A

A
x
T

G

G

L
x
A

S
|
S

G

G

P

T

G

N

R

A

I

V

A

R

A

Y

G

G

A

T

L

M

R

R

D

D

H

N

V

V

L

I

G

N

V

L

R

E

L

V

N

L

G

P

R

D

G

G

E

R

C

L

L

L

S

H

F

T

G

A

G

G

-

R

-

G

-

R

Q

H

M

Y

I

R

K

K

N

S

V

A

A

A

G

G

Y

Y

D

N

V

L

S

T

R

G

L

L

M

F

A

V

G

G

S

S

L
x
E

G
|
G

I

T

L

L

G

G

V

I

L
x
I

L

T

E

S

V

T

S

T

L

L

K

R

V

L

L

H

P

P

R

A

P

P

P

E

A

A

S

-

V

-

T

T

I

V

S

A

L

A

D

T

C

C

A

A

binding flavin-adenine dinucleotide: L90 (= L50), P132 (= P92), G133 (≠ A96), A134 (≠ P97), G140 (= G103), M141 (= M104), A143 (= A106), T144 (≠ A107), A146 (≠ L109), S147 (= S110), E200 (≠ L160), G201 (= G161), I206 (≠ L166)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 32, 68, 70, 71, 72, 73, 74, 78, 79, 322, 413, 414, 450
binding 3-methyl-2-oxobutanoic acid: 318, 369, 376, 414
binding manganese (ii) ion: 369, 376, 413

8jdpA Crystal structure of h405a mldhd in complex with d-2-hydroxyisovaleric acid (see paper)
27% identity, 39% coverage: 45:188/368 of query aligns to 85:226/455 of 8jdpA

query
sites
8jdpA

G

G

D

V

L

C

L

I

D

N

L
|
L

R

T

P

H

L

M

A

D

G

Q

V

I

R

T

S

E

Y

L

E

N

P

T

T

E

E

D

L

F

V

S

V

V

T

V

V

V

R

E

A

P

G

G

E

V

P

T

L

R

A

K

R

A

L

L

E

N

A

T

L

H

L

L

A

R

D

D

R

S

G

G

Q

L

H

W

L

F

A

P

F

V

E

D

P
|
P

P

-

R

-

F

-

A
x
G

P
x
A

G

D

G

A

T

S

V

L

G

C

G
|
G

M
|
M

V

A

A
|
A

A
x
T

G

G

L
x
A

S
|
S

G

G

P

T

G

N

R

A

I

V

A

R

A

Y

G

G

A

T

L

M

R

R

D

D

H

N

V

V

L

I

G

N

V

L

R

E

L

V

N

L

G

P

R

D

G

G

E

R

C

L

L

L

S

H

F

T

G

A

G

G

-

R

-

G

-

R

Q

H

M

Y

I

R

K

K

N

S

V

A

A

A

G

G

Y

Y

D

N

V

L

S

T

R

G

L

L

M

F

A

V

G

G

S

S

L
x
E

G
|
G

I

T

L

L

G

G

V

I

L
x
I

L

T

E

S

V

T

S

T

L

L

K

R

V

L

L

H

P

P

R

A

P

P

P

E

A

A

S

-

V

-

T

T

I

V

S

A

L

A

D

T

C

C

A

A

binding flavin-adenine dinucleotide: L90 (= L50), P132 (= P92), G133 (≠ A96), A134 (≠ P97), G140 (= G103), M141 (= M104), A143 (= A106), T144 (≠ A107), A146 (≠ L109), S147 (= S110), E200 (≠ L160), G201 (= G161), I206 (≠ L166)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 369, 413, 450
binding deaminohydroxyvaline: 319, 414

8jdvA Crystal structure of mldhd in complex with 2-ketohexanoic acid (see paper)
27% identity, 39% coverage: 45:188/368 of query aligns to 85:226/454 of 8jdvA

query
sites
8jdvA

G

G

D

V

L

C

L

I

D

N

L
|
L

R

T

P

H

L

M

A

D

G

Q

V

I

R

T

S

E

Y

L

E

N

P

T

T

E

E

D

L

F

V

S

V

V

T

V

V

V

R

E

A

P

G

G

E

V

P

T

L

R

A

K

R

A

L

L

E

N

A

T

L

H

L

L

A

R

D

D

R

S

G

G

Q

L

H

W

L

F

A

P

F

V

E

D

P
|
P

P

-

R

-

F

-

A
x
G

P
x
A

G

D

G

A

T

S

V

L

G

C

G
|
G

M
|
M

V

A

A
|
A

A
x
T

G

G

L
x
A

S
|
S

G

G

P

T

G

N

R

A

I

V

A

R

A

Y

G

G

A

T

L

M

R

R

D

D

H

N

V

V

L

I

G

N

V

L

R

E

L

V

N

L

G

P

R

D

G

G

E

R

C

L

L

L

S

H

F

T

G

A

G

G

-

R

-

G

-

R

Q

H

M

Y

I

R

K

K

N

S

V

A

A

A

G

G

Y

Y

D

N

V

L

S

T

R

G

L

L

M

F

A

V

G

G

S

S

L
x
E

G
|
G

I

T

L

L

G

G

V

I

L
x
I

L

T

E

S

V

T

S

T

L

L

K

R

V

L

L

H

P

P

R

A

P

P

P

E

A

A

S

-

V

-

T

T

I

V

S

A

L

A

D

T

C

C

A

A

binding flavin-adenine dinucleotide: L90 (= L50), P132 (= P92), G133 (≠ A96), A134 (≠ P97), G140 (= G103), M141 (= M104), A143 (= A106), T144 (≠ A107), A146 (≠ L109), S147 (= S110), E200 (≠ L160), G201 (= G161), I206 (≠ L166)

Sites not aligning to the query:

binding 2-Ketohexanoic acid: 75, 317, 321, 368, 375, 413
binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 321, 322, 412, 413, 449
binding manganese (ii) ion: 368, 375, 412

Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
26% identity, 53% coverage: 4:199/368 of query aligns to 104:298/521 of Q8N465

query
sites
Q8N465

L

L

S

R

P

P

S

R

D

T

A
x
S

T

E

E

E

L

V

R

S

L

H

V

I

E

L

Q

R

V

H

L

C

E

H

A

E

R

R

N

N

E

L

G

A

R

-

L

V

E
x
N

I

P

R

Q

G

G

G
|
G

G

N

T

T

K

G

R

M

F

V

Y

G

G

G

G

S

A

V

P

P

H

V

G

F

D

D

-

E

-

I

L
x
I

L

L

D

S

L

T

R

A

P

R

L
x
M

A

N

G

R

V

V

R

L

S

S

Y

F

E

H

P

S

T

V

E

S

L

G

V

I

V

L

T

V

V

C

R
x
Q

A
|
A

G
|
G

E
x
C

P
x
V

L
|
L

A
x
E

R
x
E

L
|
L

E
x
S

A
x
R

L
x
Y

L
x
V

A
x
E

D
x
E

R
|
R

G
x
D

Q

-

H

-

L

-

A
x
F

F
x
I

E
x
M

P
|
P

P
x
L

R
x
D

F
x
L

A
x
G

P
x
A

G
x
K

G
|
G

T
x
S

-
x
C

-
x
H

V
x
I

G
|
G

M
x
N

V
|
V

A
|
A

A
x
T

G
x
N

L
x
A

S
x
G

G
|
G

P
x
L

G
x
R

R
x
F

I
x
L

A
x
R

A
x
Y

G
|
G

A
x
S

L
|
L

R
x
H

D
x
G

H
x
T

V
|
V

L
|
L

G
|
G

V
x
L

R
x
E

L
x
V

N
x
L

G
x
A

R
x
D

G
|
G

E
x
T

C
x
V

L
|
L

S
x
D

F
x
C

G
x
L

G
x
T

Q
x
S

M
x
L

I
x
R

K
|
K

N
x
D

V
x
N

A
x
T

G
|
G

Y
|
Y

D
|
D

V
x
L

S
x
K

R
x
Q

L
|
L

M
x
F

A
x
I

G
|
G

S
|
S

L
x
E

G
|
G

I
x
T

L
|
L

G
|
G

V
x
I

L
x
I

L
x
T

E
x
T

V
|
V

S
|
S

L
x
I

K
x
L

V
x
C

L
x
P

P
|
P

R
x
K

P
|
P

P
x
R

A
|
A

S
x
V

V
x
N

T
x
V

I
x
A

S
x
F

L
|
L

D
x
G

C
|
C

A
x
P

T
x
G

Q
x
F

A
|
A

D
x
E

A
x
V

L
|
L

R
x
Q

R
x
T

L
x
F

A
x
S

A
x
T

S109 (≠ A9) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
N127 (≠ E29) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
G131 (= G33) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
I147 (≠ L47) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
M153 (≠ L53) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
C172 (≠ E72) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
P189 (= P92) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
A205 (= A106) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
A231 (≠ G132) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
G233 (= G134) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734

Sites not aligning to the query:

15 R → G: in dbSNP:rs4675887
169:521 natural variant: Missing (in D2HGA1; uncertain significance)
375 D → Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
386 binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; R→A: Loss of catalytic activity.
390 binding (R)-2-hydroxyglutarate; binding (R)-malate; T→A: Significantly reduced catalytic activity.
399 V → M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)
401 binding (R)-2-hydroxyglutarate; binding (R)-malate; K→A: Loss of catalytic activity.
419 R → H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
426 A → T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
434 binding Zn(2+); H→A: Loss of catalytic activity.
436 G → V: slight reduction in catalytic activity
439 N → D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
441 binding Zn(2+); H→A: Loss of catalytic activity.
443 binding (R)-2-hydroxyglutarate; N→A: Significantly reduced catalytic activity.
444 V → A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
446 A → V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
475 binding Zn(2+); E→A: Loss of catalytic activity.
476 binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; H→A: Loss of catalytic activity.
477 G → R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640

Query Sequence

>WP_011828560.1 NCBI__GCF_000015725.1:WP_011828560.1
MEPLSPSDATELRLVEQVLEARNEGRRLEIRGGGTKRFYGGAPHGDLLDLRPLAGVRSYE
PTELVVTVRAGEPLARLEALLADRGQHLAFEPPRFAPGGTVGGMVAAGLSGPGRIAAGAL
RDHVLGVRLLNGRGECLSFGGQMIKNVAGYDVSRLMAGSLGILGVLLEVSLKVLPRPPAS
VTISLDCATQADALRRLAAWASQPLSIGATAWHDGHLRVRLDGAAAAVRAARVALGGDEL
HERAAASWWTSVRDQLHPFFDLDPSAQLRGVRLWRLSVPRGVPPLELSGETFVEWGGALR
WLRSREPIACVRQAAARVGGHATLMRGDGREAGPFSPLSPALLQLHHRLKQAFDPERLFN
PGRLHEGL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory