SitesBLAST
Comparing WP_011840076.1 NCBI__GCF_000015985.1:WP_011840076.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
8y46B SDR family oxidoreductase (see paper)
59% identity, 98% coverage: 4:235/236 of query aligns to 5:247/248 of 8y46B
8y46A SDR family oxidoreductase (see paper)
59% identity, 98% coverage: 4:235/236 of query aligns to 8:250/251 of 8y46A
8y4jA SDR family oxidoreductase (see paper)
59% identity, 98% coverage: 4:235/236 of query aligns to 6:248/249 of 8y4jA
8y11A Crystal structure of l-2-keto-3-deoxyfuconate 4-dehydrogenase bound to NAD(h) and sulfate ion (see paper)
59% identity, 98% coverage: 4:235/236 of query aligns to 6:248/249 of 8y11A
- binding nicotinamide-adenine-dinucleotide: A12 (= A10), Q15 (= Q13), G16 (= G14), I17 (= I15), D36 (= D34), I37 (≠ L35), D57 (= D51), V58 (= V52), C80 (= C74), I130 (= I124), Y146 (= Y140), K150 (= K144), P176 (= P170), G177 (= G171), T178 (= T172), I179 (≠ V173), S181 (= S175), P182 (= P176), S183 (= S177), R187 (= R181)
D4A1J4 Dehydrogenase/reductase SDR family member 6; (R)-beta-hydroxybutyrate dehydrogenase; 3-hydroxybutyrate dehydrogenase type 2; 4-oxo-L-proline reductase; Oxidoreductase UCPA; Short chain dehydrogenase/reductase family 15C member 1; EC 1.1.1.-; EC 1.1.1.30; EC 1.1.1.104 from Rattus norvegicus (Rat) (see paper)
47% identity, 100% coverage: 1:236/236 of query aligns to 4:245/245 of D4A1J4
- Y147 (= Y140) mutation to F: Loss of function.
Q8JZV9 Dehydrogenase/reductase SDR family member 6; (R)-beta-hydroxybutyrate dehydrogenase; 3-hydroxybutyrate dehydrogenase type 2; 4-oxo-L-proline reductase; Oxidoreductase UCPA; Short chain dehydrogenase/reductase family 15C member 1; EC 1.1.1.-; EC 1.1.1.30; EC 1.1.1.104 from Mus musculus (Mouse) (see paper)
47% identity, 99% coverage: 4:236/236 of query aligns to 7:245/245 of Q8JZV9
- Y147 (= Y140) active site, Proton acceptor; mutation to F: Loss of function.
Q9BUT1 Dehydrogenase/reductase SDR family member 6; (R)-beta-hydroxybutyrate dehydrogenase; 3-hydroxybutyrate dehydrogenase type 2; 4-oxo-L-proline reductase; Oxidoreductase UCPA; Short chain dehydrogenase/reductase family 15C member 1; EC 1.1.1.-; EC 1.1.1.30; EC 1.1.1.104 from Homo sapiens (Human) (see 4 papers)
48% identity, 91% coverage: 23:236/236 of query aligns to 26:245/245 of Q9BUT1
- D37 (= D34) binding NAD(+)
- D58 (= D51) binding NAD(+)
- N70 (≠ A63) to S: in dbSNP:rs1054707
- K151 (= K144) binding NAD(+)
- VDTPS 180:184 (≠ VESPS 173:177) binding NAD(+)
Sites not aligning to the query:
2ag5A Crystal structure of human dhrs6 (see paper)
48% identity, 91% coverage: 23:236/236 of query aligns to 26:245/246 of 2ag5A
- active site: S133 (= S126), Y147 (= Y140), K151 (= K144), R192 (≠ T185)
- binding nicotinamide-adenine-dinucleotide: D37 (= D34), I38 (≠ L35), D58 (= D51), V59 (= V52), V81 (≠ C74), G83 (= G76), L104 (≠ I97), Y147 (= Y140), K151 (= K144), P177 (= P170), V180 (= V173), T182 (≠ S175), S184 (= S177)
- binding sulfate ion: R144 (= R137), R188 (= R181), F202 (= F195), R205 (= R198)
Sites not aligning to the query:
2cfcA Structural basis for stereo selectivity in the (r)- and (s)- hydroxypropylethane thiosulfonate dehydrogenases (see paper)
41% identity, 98% coverage: 4:235/236 of query aligns to 3:248/250 of 2cfcA
- active site: G13 (= G14), S142 (= S126), Y155 (= Y140), K159 (= K144)
- binding (2-[2-ketopropylthio]ethanesulfonate: F149 (≠ A134), R152 (= R137), Y155 (= Y140), W195 (≠ E180), R196 (= R181)
- binding nicotinamide-adenine-dinucleotide: G9 (≠ A10), S12 (≠ Q13), G13 (= G14), N14 (≠ I15), D33 (= D34), L34 (= L35), A59 (vs. gap), D60 (= D51), V61 (= V52), N87 (≠ C74), A88 (= A75), G89 (= G76), I140 (= I124), P185 (= P170), G186 (= G171), M187 (≠ T172), I188 (≠ V173), T190 (≠ S175), P191 (= P176), M192 (≠ S177), T193 (≠ L178)
Q56840 2-(R)-hydroxypropyl-CoM dehydrogenase; R-HPCDH; 2-[(R)-2-hydroxypropylthio]ethanesulfonate dehydrogenase; Aliphatic epoxide carboxylation component III; Epoxide carboxylase component III; RHPCDH1; EC 1.1.1.268 from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) (see 4 papers)
41% identity, 98% coverage: 4:235/236 of query aligns to 3:248/250 of Q56840
- SGN 12:14 (≠ QGI 13:15) binding NAD(+)
- D33 (= D34) binding NAD(+)
- DV 60:61 (= DV 51:52) binding NAD(+)
- N87 (≠ C74) binding NAD(+)
- S142 (= S126) mutation to A: Retains weak activity. 120-fold decrease in kcat.; mutation to C: Loss of activity.
- R152 (= R137) binding 2-oxopropyl-coenzyme M; mutation to A: Almost loss of activity with the natural substrate 2-KPC, but does not affect activity with 2-butanone as substrate.
- Y155 (= Y140) mutation Y->E,F: Loss of activity.
- K159 (= K144) mutation to A: Loss of activity.
- R179 (= R164) mutation to A: Loss of activity.
- IETPM 188:192 (≠ VESPS 173:177) binding NAD(+)
- WR 195:196 (≠ ER 180:181) binding 2-oxopropyl-coenzyme M
- R196 (= R181) mutation to A: Almost loss of activity with the natural substrate 2-KPC, but does not affect activity with 2-butanone as substrate.
- R203 (= R192) mutation to A: Slight decrease in catalytic efficiency.
- R209 (= R198) mutation to A: Does not affect catalytic efficiency.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
37% identity, 100% coverage: 1:235/236 of query aligns to 5:253/255 of 5itvA
- active site: G18 (= G14), S141 (= S126), Y154 (= Y140), K158 (= K144)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (≠ A10), S17 (≠ Q13), G18 (= G14), I19 (= I15), D38 (= D34), I39 (≠ L35), T61 (vs. gap), I63 (≠ V52), N89 (≠ C74), G91 (= G76), T139 (≠ I124), S141 (= S126), Y154 (= Y140), K158 (= K144), P184 (= P170), G185 (= G171), I186 (≠ T172), I187 (≠ V173)
6qheA Alcohol dehydrogenase from arthrobacter sp. Ts-15 in complex with NAD+
34% identity, 100% coverage: 1:236/236 of query aligns to 5:256/261 of 6qheA
- binding nicotinamide-adenine-dinucleotide: G14 (≠ A10), M17 (≠ Q13), G18 (= G14), M19 (≠ I15), D38 (= D34), R39 (vs. gap), D63 (= D51), I64 (≠ V52), A90 (≠ C74), A91 (= A75), S142 (= S126), Y156 (= Y140), K160 (= K144), P186 (= P170), G187 (= G171), M189 (≠ V173), T191 (≠ S175), P192 (= P176), M193 (≠ S177)
4nbtA Crystal structure of fabg from acholeplasma laidlawii (see paper)
37% identity, 99% coverage: 1:233/236 of query aligns to 3:236/239 of 4nbtA
- active site: G16 (= G14), S132 (= S126), Y145 (= Y140), K149 (= K144)
- binding nicotinamide-adenine-dinucleotide: G12 (≠ A10), K15 (≠ Q13), G16 (= G14), L17 (≠ I15), D36 (= D34), L37 (= L35), L52 (= L50), N53 (≠ D51), V54 (= V52), N80 (≠ C74), A81 (= A75), G82 (= G76), I130 (= I124), S132 (= S126), Y145 (= Y140), K149 (= K144), P177 (= P170), G178 (= G171), I180 (≠ V173), T182 (≠ S175)
Q9KJF1 (2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA dehydrogenase subunit BbsD; (S,R)-2-(alpha-hydroxybenzyl)succinyl-CoA dehydrogenase subunit BbsD; EC 1.1.1.429 from Thauera aromatica (see 2 papers)
34% identity, 99% coverage: 1:233/236 of query aligns to 3:242/248 of Q9KJF1
- S15 (≠ Q13) binding NAD(+)
- D36 (= D34) binding NAD(+)
- D62 (= D51) binding NAD(+)
- I63 (≠ V52) binding NAD(+)
- N89 (≠ C74) binding NAD(+)
- Y153 (= Y140) binding NAD(+)
- K157 (= K144) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
7pcsB Structure of the heterotetrameric sdr family member bbscd (see paper)
34% identity, 99% coverage: 1:233/236 of query aligns to 2:241/247 of 7pcsB
- binding nicotinamide-adenine-dinucleotide: G11 (≠ A10), M16 (≠ I15), D35 (= D34), I36 (≠ L35), I62 (≠ V52), N88 (≠ C74), G90 (= G76), I138 (= I124), S140 (= S126), Y152 (= Y140), K156 (= K144), I185 (≠ V173)
5ts3A Crystal structure of a 3-oxoacyl-[acyl-carrier protein] reductase with bound NAD from brucella melitensis
39% identity, 98% coverage: 2:233/236 of query aligns to 18:261/265 of 5ts3A
- active site: G30 (= G14), S158 (= S126), V168 (≠ D136), Y172 (= Y140), K176 (= K144)
- binding nicotinamide-adenine-dinucleotide: G26 (≠ A10), R29 (≠ Q13), G30 (= G14), I31 (= I15), D50 (= D34), L51 (= L35), D77 (= D51), V78 (= V52), N101 (≠ C74), V129 (≠ I97), T156 (≠ I124), Y172 (= Y140), K176 (= K144), P202 (= P170), I205 (≠ V173), T207 (≠ S175)
7djsD Crystal structure of isopiperitenol dehydrogenase from pseudomonas aeruginosa complexed with NAD
40% identity, 98% coverage: 4:235/236 of query aligns to 6:249/251 of 7djsD
- binding nicotinamide-adenine-dinucleotide: G12 (≠ A10), G16 (= G14), I17 (= I15), D36 (= D34), L37 (= L35), C61 (vs. gap), D62 (= D51), V63 (= V52), N89 (≠ C74), A90 (= A75), T140 (≠ I124), S142 (= S126), Y155 (= Y140), K159 (= K144), A186 (≠ G171), V187 (≠ T172)
A7IQH5 2-(S)-hydroxypropyl-CoM dehydrogenase 3; S-HPCDH 3; 2-[(S)-2-hydroxypropylthio]ethanesulfonate dehydrogenase 3; Aliphatic epoxide carboxylation component IV; Epoxide carboxylase component IV; SHPCDH3; EC 1.1.1.269 from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) (see 2 papers)
36% identity, 90% coverage: 24:235/236 of query aligns to 28:253/255 of A7IQH5
- D38 (= D34) binding NAD(+)
- DV 64:65 (= DV 51:52) binding NAD(+)
- N91 (≠ C74) binding NAD(+)
- S143 (= S126) binding (S)-2-hydroxypropyl-coenzyme M; mutation to A: Retains very weak activity.
- Y156 (= Y140) binding (S)-2-hydroxypropyl-coenzyme M; mutation to A: Retains some activity but with more than 2200-fold decrease in catalytic efficiency.; mutation to F: Loss of activity.
- K160 (= K144) binding NAD(+); mutation to A: Loss of activity.
- T188 (= T172) binding (S)-2-hydroxypropyl-coenzyme M
- VTSTG 189:193 (≠ VESPS 173:177) binding NAD(+)
- R211 (≠ F195) mutation to A: Severely impaired in the oxidation of S-HPC or reduction of 2-KPC but largely unaffected in the oxidation and reduction of aliphatic alcohols and ketones.
- K214 (≠ R198) mutation to A: Severely impaired in the oxidation of S-HPC or reduction of 2-KPC but largely unaffected in the oxidation and reduction of aliphatic alcohols and ketones.
- Y215 (≠ Q199) binding (S)-2-hydroxypropyl-coenzyme M
Sites not aligning to the query:
4ituA Crystal structure of s-2-hydroxypropyl coenzyme m dehydrogenase (s- hpcdh) bound to s-hpc and nadh (see paper)
36% identity, 90% coverage: 24:235/236 of query aligns to 26:251/253 of 4ituA
- active site: N113 (= N98), S141 (= S126), Y154 (= Y140), K158 (= K144)
- binding 2-{[(2S)-2-hydroxypropyl]sulfanyl}ethanesulfonic acid: S141 (= S126), Y154 (= Y140), T186 (= T172), R209 (≠ F195), Y213 (≠ Q199)
- binding 1,4-dihydronicotinamide adenine dinucleotide: D36 (= D34), L37 (= L35), D62 (= D51), V63 (= V52), N89 (≠ C74), V112 (≠ I97), F139 (≠ I124), S141 (= S126), Y154 (= Y140), K158 (= K144), P184 (= P170), T186 (= T172), V187 (= V173), T190 (≠ P176), M192 (≠ L178)
Sites not aligning to the query:
3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
34% identity, 100% coverage: 1:236/236 of query aligns to 6:246/247 of 3op4A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (≠ A10), S17 (≠ G12), R18 (≠ Q13), I20 (= I15), T40 (≠ A32), N62 (≠ D51), V63 (= V52), N89 (≠ C74), A90 (= A75), I92 (≠ V77), V139 (≠ I124), S141 (= S126), Y154 (= Y140), K158 (= K144), P184 (= P170), G185 (= G171), I187 (≠ V173), T189 (≠ S175), M191 (≠ S177)
Query Sequence
>WP_011840076.1 NCBI__GCF_000015985.1:WP_011840076.1
MQEKTVLITAAGQGIGRACAERFLAAGARVLASDLDARLLDGLAAEGHALDVRDGAAVEA
LVAGLGPVDVLVNCAGVVHGGTILDQTEAELDLALDINLRGTIRTIRAVLPGMLAAGSGS
IVNIASVVSSLKGAPDRCVYGTSKAAVIGLTKSVAADFVSRGIRCNAICPGTVESPSLRE
RLGATGDAEAARAAFVARQPMGRLGTPEEIADLALWLACSGYTTGQAIAIDGGWTI
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory