SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011840643.1 NCBI__GCF_000015985.1:WP_011840643.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P50457 4-aminobutyrate aminotransferase PuuE; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; EC 2.6.1.19 from Escherichia coli (strain K12) (see paper)
59% identity, 99% coverage: 1:420/425 of query aligns to 1:420/421 of P50457

query
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P50457

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K267 (= K267) mutation to A: No GABA-AT activity.

1sffA Structure of gamma-aminobutyrate aminotransferase complex with aminooxyacetate (see paper)
54% identity, 99% coverage: 3:424/425 of query aligns to 3:425/425 of 1sffA

query
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1sffA

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active site: V18 (= V18), Y137 (≠ F137), E205 (= E205), D238 (= D238), Q241 (= Q241), K267 (= K267), T296 (= T296), R397 (= R396)
binding 4'-deoxy-4'-acetylyamino-pyridoxal-5'-phosphate: Q78 (= Q78), G110 (= G110), S111 (≠ A111), Y137 (≠ F137), H138 (= H138), R140 (= R140), E205 (= E205), D238 (= D238), V240 (= V240), Q241 (= Q241), K267 (= K267), T296 (= T296)
binding sulfate ion: N152 (≠ E152), Y393 (≠ G392)

1sf2A Structure of e. Coli gamma-aminobutyrate aminotransferase (see paper)
54% identity, 99% coverage: 3:424/425 of query aligns to 3:425/425 of 1sf2A

query
sites
1sf2A

N

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active site: V18 (= V18), Y137 (≠ F137), E205 (= E205), D238 (= D238), Q241 (= Q241), K267 (= K267), T296 (= T296), R397 (= R396)
binding pyridoxal-5'-phosphate: G110 (= G110), S111 (≠ A111), Y137 (≠ F137), H138 (= H138), E205 (= E205), D238 (= D238), V240 (= V240), Q241 (= Q241), K267 (= K267)
binding sulfate ion: N152 (≠ E152), Y393 (≠ G392)

P22256 4-aminobutyrate aminotransferase GabT; 5-aminovalerate transaminase; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; L-AIBAT; EC 2.6.1.19; EC 2.6.1.48 from Escherichia coli (strain K12) (see 2 papers)
54% identity, 99% coverage: 3:424/425 of query aligns to 4:426/426 of P22256

query
sites
P22256

N

N

A

K

D

E

V

L

S

M

R

Q

R

R

T

S

E

Q

A

A

V

I

S

P

R

R

G

G

V

V

G

G

M

Q

Q

I

T

H

Q

P

I

I

Y

F

A

A

D

D

R

R

A

A

L

E

N

N

A

C

E

R

I

V

W

W

D

D

I

V

E

E

G

G

R

R

R

E

Y

Y

I

L

D

D

F

F

A

A

A

G

G

G

I
|
I

A

A

V

V

V

L

N

N

T

T

G

G

H

H

C

L

H

H

P

P

K

K

V

V

M

V

A

A

V

V

A

E

E

A

Q

Q

M

L

G

K

R

K

F

L

T

S

H

H

T

T

C

C

H

F

Q

Q

V

V

L

L

P

A

Y

Y

E

E

N

P

Y

Y

I

L

R

E

L

L

A

C

E

E

R

I

L

M

N

N

A

Q

A

K

A

V

P

P

G

G

D

D

F

F

A

A

K

K

T

T

V

L

F

L

V

V

T

T

G
|
G

A
x
S

E

E

S

A

V

V

E

E

N

N

A

A

I

V

K

K

I

I

A

A

R

R

I

A

H

A

T

T

G

K

R

R

S

S

A

G

V

T

V

I

A

A

F

F

G

S

G

G

G

A

F

Y

H

H

G

G

R

R

T

T

F

H

M

Y

T

T

M

L

S

A

L

L

T

T

G

G

K

K

V

V

E

N

P

P

Y

Y

K

S

K

A

G

G

F

M

G

G

T

L

M

M

M

P

P

G

E

H

V

V

Y

Y

H

R

V

A

P

L

F

Y

P

P

Q

C

A

P

L

L

H

H

G

G

I

I

S

S

T

E

E

D

A

A

M

I

A

A

A

S

L

I

A

H

R

R

L

I

F

F

K

K

A

N

D

D

L

A

D

A

P

P

G

E

R

D

V

I

A

A

I

I

I

V

F

I

E

E

P

P

V

V

Q

Q

G

G

E
|
E

G

G

F

F

Y

Y

P

A

A

S

P

S

P

P

D

A

L

F

V

M

R

Q

A

R

I

L

R

R

Q

A

L

L

C

C

D

D

A

E

H

H

G

G

I

I

V

M

M

L

I

I

A

A

D

D

E

E

V
|
V

Q
|
Q

T

S

G

G

F

A

A

G

R

R

T

T

G

G

T

T

L

L

F

F

A

A

M

M

H

E

G

Q

Y

M

D

G

V

V

A

A

P

P

D

D

L

L

V

T

T

T

M

F

A

A

K
|
K

G

S

L

I

G

A

G

G

L

F

P

P

I

L

A

A

A

G

V

V

T

T

G

G

R

R

A

A

E

E

I

V

M

M

D

D

A

A

A

V

H

A

P

P

G

G

L

L

G

G

T
|
T

Y

Y

G

A

G

G

N

N

P

P

L

I

G

A

I

C

A

V

A

A

H

L

A

E

V

V

L

L

D

K

V

V

I

F

E

E

E

Q

E

E

N

N

L

L

C

L

A

Q

R

K

A

A

T

N

E

D

L

L

G

G

S

Q

R

K

L

L

K

K

Q

D

K

G

L

L

A

L

E

A

I

I

R

A

A

E

T

K

A

H

P

P

E

E

I

I

A

G

D

D

I

V

R

R

G

G

P

L

G

G

F

A

M

M

V

I

G

A

A

I

E

E

-

L

F

F

A

E

D

D

P

G

G

D

S

H

K

N

A

K

P

P

D

D

A

A

G

K

F

L

T

T

N

A

R

E

V

I

R

V

E

A

E

R

A

A

L

R

K

D

R

K

G

G

L

L

I

I

L

L

T

S

C

C

G

G

V

P

H

Y

G

Y

N

N

V

V

I

L

R

R

F

I

L

L

A

V

P

P

I

L

T

T

I

I

P

E

E

D

P

A

H

Q

F

I

A

R

E

Q

A

G

L

L

E

E

I

I

L

I

E

S

A

Q

S

C

V

F

A

D

A

E

A

A

R

K

Q

Q

I50 (= I49) mutation to Q: 3-fold decrease in catalytic activity and 12-fold decrease in affinity for GABA.
GS 111:112 (≠ GA 110:111) binding pyridoxal 5'-phosphate
E211 (= E210) mutation to S: 100-fold decrease in catalytic activity and 15-fold decrease in affinity for GABA.
V241 (= V240) mutation to A: 25-fold decrease in catalytic activity and 5-fold decrease in affinity for GABA.
Q242 (= Q241) binding pyridoxal 5'-phosphate
K268 (= K267) modified: N6-(pyridoxal phosphate)lysine
T297 (= T296) binding pyridoxal 5'-phosphate

1szkA The structure of gamma-aminobutyrate aminotransferase mutant: e211s (see paper)
53% identity, 99% coverage: 3:424/425 of query aligns to 3:425/425 of 1szkA

query
sites
1szkA

N

N

A

K

D

E

V

L

S

M

R

Q

R

R

T

S

E

Q

A

A

V

I

S

P

R

R

G

G

V
|
V

G

G

M

Q

Q

I

T

H

Q

P

I

I

Y

F

A

A

D

D

R

R

A

A

L

E

N

N

A

C

E

R

I

V

W

W

D

D

I

V

E

E

G

G

R

R

R

E

Y

Y

I

L

D

D

F

F

A

A

A

G

G

G

I

I

A

A

V

V

V

L

N

N

T

T

G

G

H

H

C

L

H

H

P

P

K

K

V

V

M

V

A

A

V

V

A

E

E

A

Q

Q

M

L

G

K

R

K

F

L

T

S

H

H

T

T

C

C

H

F

Q

Q

V

V

L

L

P

A

Y

Y

E

E

N

P

Y

Y

I

L

R

E

L

L

A

C

E

E

R

I

L

M

N

N

A

Q

A

K

A

V

P

P

G

G

D

D

F

F

A

A

K

K

T

T

V

L

F

L

V

V

T

T

G
|
G

A
x
S

E

E

S

A

V

V

E

E

N

N

A

A

I

V

K

K

I

I

A

A

R

R

I

A

H

A

T

T

G

K

R

R

S

S

A

G

V

T

V

I

A

A

F

F

G

S

G

G

G

A

F
x
Y

H
|
H

G

G

R

R

T

T

F

H

M

Y

T

T

M

L

S

A

L

L

T

T

G

G

K

K

V

V

E

N

P

P

Y

Y

K

S

K

A

G

G

F

M

G

G

T

L

M

M

M

P

P

G

E

H

V

V

Y

Y

H

R

V

A

P

L

F

Y

P

P

Q

C

A

P

L

L

H

H

G

G

I

I

S

S

T

E

E

D

A

A

M

I

A

A

A

S

L

I

A

H

R

R

L

I

F

F

K

K

A

N

D

D

L

A

D

A

P

P

G

E

R

D

V

I

A

A

I

I

I

V

F

I

E
|
E

P

P

V

V

Q

Q

G

G

E

S

G

G

F

F

Y

Y

P

A

A

S

P

S

P

P

D

A

L

F

V

M

R

Q

A

R

I

L

R

R

Q

A

L

L

C

C

D

D

A

E

H

H

G

G

I

I

V

M

M

L

I

I

A

A

D
|
D

E

E

V
|
V

Q
|
Q

T

S

G

G

F

A

A

G

R

R

T

T

G

G

T

T

L

L

F

F

A

A

M

M

H

E

G

Q

Y

M

D

G

V

V

A

A

P

P

D

D

L

L

V

T

T

T

M

F

A

A

K
|
K

G

S

L

I

G

A

G

G

L

F

P

P

I

L

A

A

A

G

V

V

T

T

G

G

R

R

A

A

E

E

I

V

M

M

D

D

A

A

A

V

H

A

P

P

G

G

L

L

G

G

T
|
T

Y

Y

G

A

G

G

N

N

P

P

L

I

G

A

I

C

A

V

A

A

H

L

A

E

V

V

L

L

D

K

V

V

I

F

E

E

E

Q

E

E

N

N

L

L

C

L

A

Q

R

K

A

A

T

N

E

D

L

L

G

G

S

Q

R

K

L

L

K

K

Q

D

K

G

L

L

A

L

E

A

I

I

R

A

A

E

T

K

A

H

P

P

E

E

I

I

A

G

D

D

I

V

R

R

G

G

P

L

G

G

F

A

M

M

V

I

G

A

A

I

E

E

-

L

F

F

A

E

D

D

P

G

G

D

S

H

K

N

A

K

P

P

D

D

A

A

G

K

F

L

T

T

N

A

R

E

V

I

R

V

E

A

E

R

A

A

L

R

K

D

R

K

G

G

L

L

I

I

L

L

T

S

C

C

G

G

V

P

H

Y

G

Y

N

N

V

V

I

L

R
|
R

F

I

L

L

A

V

P

P

I

L

T

T

I

I

P

E

E

D

P

A

H

Q

F

I

A

R

E

Q

A

G

L

L

E

E

I

I

L

I

E

S

A

Q

S

C

V

F

A

D

A

E

A

A

R

K

Q

Q

active site: V18 (= V18), Y137 (≠ F137), E205 (= E205), D238 (= D238), Q241 (= Q241), K267 (= K267), T296 (= T296), R397 (= R396)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G110 (= G110), S111 (≠ A111), Y137 (≠ F137), H138 (= H138), E205 (= E205), D238 (= D238), V240 (= V240), Q241 (= Q241), K267 (= K267)

3q8nC Crystal structure of 4-aminobutyrate transaminase from mycobacterium smegmatis (see paper)
47% identity, 99% coverage: 2:423/425 of query aligns to 16:439/439 of 3q8nC

query
sites
3q8nC

L

I

N

S

A

Q

D

E

V

L

S

Q

R

A

R

R

R

K

T

Q

E

S

A

A

V

V

S

A

R

A

G

G

V
|
V

G

G

M

V

Q

T

T

L

Q

P

I

V

Y

Y

A

V

D

V

R

A

A

A

L

G

N

G

A

G

E

V

I

L

W

A

D

D

I

A

E

D

G

G

R

N

R

Q

Y

L

I

I

D

D

F

F

A

G

A

S

G

G

I

I

A

A

V

V

V

T

N

T

T

V

G

G

H

N

C

S

H

A

P

P

K

A

V

V

M

V

A

D

A

A

V

V

A

T

E

Q

Q

Q

M

V

G

A

R

A

F

F

T

T

H

H

T

T

C

C

H

F

Q

M

V

V

L

T

P

P

Y

Y

E

E

N

G

Y

Y

I

V

R

K

L

V

A

A

E

E

R

H

L

L

N

N

A

R

A

L

A

T

P

P

G

G

D

D

F

H

A

E

K

K

K

R

T

T

V

A

F

L

V

F

T

N

T

S

G
|
G

A
|
A

E

E

S

A

V

V

E

E

N

N

A

A

I

V

K

K

I

I

A

A

R

R

I

A

H

Y

T

T

G

R

R

R

S

Q

A

A

V

V

A

V

F

F

G

D

G

H

G

A

F
x
Y

H

H

G

G

R

R

T

T

F

N

M

L

T

T

M

M

S

A

L

M

T

T

G

A

K

K

V

N

E

Q

P

P

Y

Y

K

K

K

H

G

G

F

F

G

G

T

P

M

F

M

A

P

N

E

E

V

V

Y

Y

H

R

V

V

P

P

F

T

P

S

Q

Y

A

P

L

F

H

R

G

D

I

G

S

E

T

T

E

D

-

G

-

A

E

A

A

A

M

A

A

A

A

H

L

A

A

L

R

D

L

L

F

I

K

N

A

K

D

Q

L

V

D

G

P

A

G

D

R

N

V

V

A

A

I

V

F

I

E
|
E

P

P

V

V

Q

H

G

G

E

E

G

G

F

F

Y

V

P

V

A

P

P

A

P

P

D

G

L

F

V

L

R

G

A

A

I

L

R

Q

Q

K

L

W

C

C

D

T

A

D

H

N

G

G

I

A

V

V

M

F

I

V

A

A

D
|
D

E

E

V
|
V

Q
|
Q

T

T

G

G

F

F

A

A

R

R

T

T

G

G

T

A

L

L

F

F

A

A

M

C

H

E

G

H

Y

E

D

N

V

V

A

V

P

P

D

D

L

L

V

I

T

V

M

T

A

A

K
|
K

G

G

L

I

G

A

G

G

L

L

P

P

I

L

A

S

A

A

V

V

T

T

G

G

R

R

A

A

E

E

I

I

M

M

D

D

A

G

A

P

H

Q

P

S

G

G

L

L

G

G

T
|
T

Y

Y

G

G

N

N

P

P

L

L

G

A

I

C

A

A

H

L

A

A

V

V

L

I

D

D

V

T

I

I

E

E

E

R

E

E

N

N

L

L

C

V

A

A

R

R

A

A

T

R

E

A

L

I

G

G

S

E

R

T

L

M

K

L

Q

S

K

R

L

L

A

G

E

A

I

L

R

A

A

A

T

A

A

D

P

P

E

R

I

I

A

G

D

E

I

V

R

R

G

G

P

R

G

G

F

A

M

M

V

I

G

A

A

V

E

E

F

L

A

V

D

K

P

P

G

G

S

T

K

T

A

E

P

P

D

D

A

A

G

D

F

L

T

T

N

K

R

R

V

V

R

A

E

A

A

A

L

H

K

A

R

Q

G

G

L

L

I

V

L

V

L

L

T

T

C

C

G

G

V

T

H

Y

G

G

N

N

V

V

I

L

R
|
R

F

F

L

L

A

P

P

P

I

L

T

S

I

M

P

P

E

D

P

H

H

L

F

L

A

D

E

E

A

G

L

L

E

D

I

I

L

L

E

A

A

A

S

V

V

F

A

A

A

E

A

V

R

K

active site: V32 (= V18), Y151 (≠ F137), E221 (= E205), D254 (= D238), Q257 (= Q241), K283 (= K267), T312 (= T296), R412 (= R396)
binding 4-oxobutanoic acid: G124 (= G110), A125 (= A111), V256 (= V240), K283 (= K267)

4atqF Gaba-transaminase a1r958 in complex with external aldimine plp-gaba adduct (see paper)
46% identity, 98% coverage: 6:421/425 of query aligns to 23:443/444 of 4atqF

query
sites
4atqF

V

L

S

A

R

E

R

R

T

S

E

A

A

V

V

V

S

A

R

A

G

G

V
|
V

G

A

M

S

Q

G

T

V

Q

P

I

V

Y

Y

A

V

D

E

R

D

A

A

L

D

N

G

A

G

E

I

I

I

W

R

D

D

I

V

E

D

G

G

R

N

R

S

Y

F

I

I

D

D

F

L

A

G

A

S

G

G

I

I

A

A

V

V

V

T

N

S

T

V

G

G

H

A

C

S

H

D

P

P

K

A

V

V

M

V

A

A

V

V

A

Q

E

E

Q

A

M

A

G

A

R

H

F

F

T

T

H

H

T

T

C

C

H

F

Q
x
M

V

V

L

T

P

P

Y

Y

E

E

N

G

Y

Y

I

V

R

A

L

V

A

T

E

E

R

Q

L

L

N

N

A

R

A

L

A

T

P

P

G

G

D

D

F

H

A

A

K

K

K

R

T

T

V

V

F

L

V

F

T

N

T

S

G
|
G

A
|
A

E

E

S

A

V

V

E

E

N

N

A

A

I

V

K

K

I

V

A

A

R

R

I

L

H

A

T

T

G

G

R

R

S

D

A

A

V

V

A

A

F

F

G

D

G

H

G

A

F
x
Y

H
|
H

G

G

R
|
R

T

T

F

N

M

L

T

T

M

M

S

A

L

L

T

T

G

A

K

K

V

A

E

M

P

P

Y

Y

K

K

K

T

G

N

F

F

G

G

T

P

M

F

M

A

P

P

E

E

V

V

Y

Y

H

R

V

M

-

P

-

M

-

S

-

Y

P

P

F

F

P

R

Q

E

A

E

L

N

H

P

G

E

I

I

S

T

T

G

E

A

E

E

A

A

M

A

A

K

A

R

L

A

A

I

R

T

L

M

F

I

K

E

A

K

D

Q

L

I

D

G

P

G

G

D

R

Q

V

V

A

A

I

I

F

I

E
|
E

P

P

V

I

Q

Q

G

G

E
|
E

G

G

F

F

Y

I

P

V

A

P

P

A

P

E

D

G

L

F

V

L

R

P

A

A

I

L

R

S

Q

E

L

W

C

A

D

K

A

E

H

K

G

G

I

I

V

V

M

F

I

I

A

A

D
|
D

E

E

V
|
V

Q
|
Q

T

S

G

G

F

F

A

C

R

R

T

T

G

G

T

E

L

W

F

F

A

A

M

V

H

D

G

H

Y

E

D

G

V

V

A

V

P

P

D

D

L

I

V

I

T

T

M

M

A

A

K
|
K

G

G

L

I

G

A

G

G

L

L

P

P

I

L

A

S

A

A

V

I

T

T

G

G

R

R

A

A

E

D

I

L

M

L

D

D

A

A

A

V

H

H

P

P

G

G

L

L

G

G

G
|
G

T
|
T

Y

Y

G

G

N

N

P

P

L

V

G

A

I

C

A

A

H

L

A

A

V

A

L

I

D

D

V

T

I

M

E

E

E

Q

E

H

N

D

L

L

C

N

A

G

R

R

A

A

T

R

E

H

L

I

G

E

S

E

R

L

L

A

K

L

Q

G

K

K

L

L

A

R

E

E

I

L

R

A

A

A

T

E

A

G

P

G

E

S

I

V

A

V

-

G

D

D

I

I

R

R

G

G

P

R

G

G

F

A

M

M

V

L

G

A

A

I

E

E

F

L

A

V

D

Q

P

P

G

G

S

S

K

K

A

E

P

P

D

N

A

A

G

E

F

L

T

T

N

K

R

A

V

V

R

A

E

A

A

C

L

L

K

K

R

E

G

G

L

V

I

I

L

I

L

L

T

T

C

C

G

G

V

T

H

Y

G

G

N

N

V

V

I

I

R
|
R

F

L

L

L

A

P

P

P

I

L

T

V

I

I

P

S

E

D

P

E

H

L

F

L

A

I

E

D

A

G

L

L

E

E

I

V

L

L

E

A

A

A

S

A

V

I

A

K

A

A

active site: V35 (= V18), Y154 (≠ F137), E226 (= E205), D259 (= D238), Q262 (= Q241), K288 (= K267), T317 (= T296), R418 (= R396)
binding gamma-amino-butanoic acid: M95 (≠ Q78), Y154 (≠ F137), R157 (= R140), E231 (= E210), K288 (= K267), G316 (= G295)
binding pyridoxal-5'-phosphate: G127 (= G110), A128 (= A111), Y154 (≠ F137), H155 (= H138), D259 (= D238), V261 (= V240)

6j2vA Gaba aminotransferase from corynebacterium glutamicum (see paper)
44% identity, 97% coverage: 6:419/425 of query aligns to 23:435/440 of 6j2vA

query
sites
6j2vA

V

L

S

D

R

E

R

R

T

Q

E

A

A

A

V

V

S

A

R

R

G

A

V
x
L

G

A

M

P

Q

G

T

L

Q

P

I

G

Y

Y

A

V

D

V

R

D

A

A

L

D

N

G

A

G

E

I

I

L

W

A

D

D

I

A

E

D

G

G

R

N

R

R

Y

F

I

I

D

D

F

L

A

A

A

S

G

G

I

I

A

A

V

V

V

T

N

T

T

V

G

G

H

G

C

S

H

N

P

A

K

A

V

V

M

A

A

K

A

A

V

V

A

G

E

A

Q

A

M

A

G

A

R

R

F

F

T

T

H

H

T

T

C

C

H

F

Q

M

V

V

L

S

P

P

Y

Y

E

E

N

T

Y

Y

I

V

R

A

L

M

A

A

E

E

R

R

L

L

N

N

A

A

A

L

A

T

P

P

G

G

D

D

F

H

A

D

K

K

T

S

V

A

F

L

V

F

T

N

T

S

G
|
G

A
|
A

E

E

S

A

V

V

E

E

N

N

A

A

I

V

K

K

I

V

A

A

R

R

I

A

H

Y

T

T

G

G

R

K

S

G

A

A

V

V

A

V

F

F

G

D

G

N

G

A

F
x
Y

H
|
H

G

G

R
|
R

T

T

F

N

M

L

T

T

M

M

S

A

L

M

T

T

G

A

K

K

V

N

E

R

P

P

Y

Y

K

K

K

S

G

G

F

F

G

G

T

P

M

L

M

A

P

A

E

D

V

V

Y

Y

H

R

V

A

P

P

F

M

P

S

Q

Y

A

P

L

L

H

R

-

D

G

G

I

L

S

S

T

G

E

P

E

E

A

A

M

A

A

E

A

R

L

A

A

I

R

S

L

V

F

I

K

E

A

S

D

Q

L

V

D

G

P

A

G

E

R

N

V

L

A

A

A

C

I

V

F

I

E
|
E

P

P

V

I

Q

Q

G

G

E
|
E

G

G

F

F

Y

I

P

V

A

P

P

A

P

P

D

G

L

F

V

L

R

A

A

A

I

I

R

S

Q

T

L

W

C

C

D

R

A

E

H

N

G

D

I

V

V

V

M

F

I

I

A

A

D
|
D

E

E

V
x
I

Q

Q

T

S

G

G

F

F

A

L

R

R

T

T

G

G

T

D

L

W

F

F

A

A

M

S

H

D

G

A

Y

E

D

G

V

V

A

I

P

P

D

D

L

V

V

I

T

T

M

T

A

A

K
|
K

G

G

L

I

G

A

G

G

L

M

P

P

I

L

A

S

A

A

V

V

T

T

G

G

R

R

A

A

E

E

I

I

M

M

D

D

A

A

A

P

H

G

P

P

G

G

G

A

L

L

G

G

G
|
G

T
|
T

Y

Y

G

G

N

N

P

P

L

V

G

A

I

C

A

A

H

L

A

A

V

A

L

I

D

E

V

V

I

M

E

E

E

Q

E

A

N

D

L

L

C

K

A

T

R

R

A

A

T

Q

E

E

L

I

G

E

S

T

R

I

L

I

K

R

Q

D

K

E

L

F

A

A

E

Q

I

L

R

S

A

A

T

F

A

-

P

P

E

E

I

V

A

A

D

E

I

I

R

R

G

G

P

R

G

G

F

A

M

M

V

M

G

A

A

I

E

E

F

L

A

I

D

D

P

A

G

T

S

G

K

R

A

-

P

P

D

N

A

A

G

A

F

L

T

T

N

A

R

A

V

V

R

A

E

A

E

R

A

A

L

K

K

A

R

E

G

G

L

V

I

L

L

L

T

T

C

C

G

G

V

T

H

D

G

G

N

N

V

V

I

I

R

R

F

L

L

L

A

P

P

P

I

L

T

V

I

I

P

A

E

E

P

D

H

T

F

L

A

R

E

D

A

G

L

L

E

Q

I

V

L

L

E

V

A

A

S

A

V

L

active site: L35 (≠ V18), Y154 (≠ F137), D256 (= D238), K285 (= K267)
binding 4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]butanoic acid: G127 (= G110), A128 (= A111), Y154 (≠ F137), H155 (= H138), R157 (= R140), E223 (= E205), E228 (= E210), D256 (= D238), I258 (≠ V240), K285 (= K267), G313 (= G295), T314 (= T296)

O58478 Alanine/serine racemase; ASR; Ala/Ser racemase; EC 5.1.1.-; EC 5.1.1.1 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
34% identity, 99% coverage: 5:425/425 of query aligns to 34:464/474 of O58478

query
sites
O58478

D

E

V

L

S

I

R

E

R

R

R

E

T

K

E

K

A

V

V

L

S

S

R

T

G

G

V

I

G

G

M

V

Q

K

T

L

-

F

Q

P

I

L

Y

V

A

P

D

K

R

R

A

G

L

F

N

G

A

P

E

F

I

I

W

E

D

D

I

V

E

D

G

G

R

N

R

V

Y

F

I

I

D

D

F

F

A

L

A

A

G

G

I

A

A

A

V

A

N

S

T

T

G

G

H

Y

C

A

H

H

P

P

K

K

V

L

M

V

A

K

A

A

V

V

A

K

E

E

Q

Q

M

V

G

E

R

L

F

I

T

Q

H

H

T

S

C

M

H

I

Q

G

V

Y

L

T

P

H

Y

S

E

E

N

R

Y

A

I

I

R

R

L

V

A

A

E

E

R

K

L

L

N

V

A

E

A

I

A

S

P

P

G

I

D

E

F

-

A

N

K

S

K

K

T

V

V

I

F

F

V

G

T

L

T

S

G

G

A

S

E

D

S

A

V

V

E

D

N

M

A

A

I

I

K

K

I

V

A

S

R

K

I

F

H

S

T

T

G

R

R

R

S

P

A

W

V

I

V

L

A

A

F

F

G

I

G

G

G

A

F

Y

H

H

G

G

R

Q

T

T

F

L

M

G

T

A

M

T

S

S

L

V

T

A

G

S

K

F

V

Q

E

V

P

S

Y

Q

K

K

K

R

G

G

F

Y

G

S

T

P

M

L

M

M

P

P

E

N

V

V

Y

F

H

W

V

I

P

P

F

Y

P

P

Q

N

A

P

L

F

H

R

G

N

I

I

S

W

-

G

-

I

-

N

-

G

T

Y

E

E

A

P

M

D

A

E

A

L

L

I

A

N

R

R

L

V

-

L

-

D

-

Y

-

L

-

E

-

Y

-

V

F

F

K

S

A

H

D

V

L

V

D

P

P

P

G

D

R

E

V

V

A

A

I

L

I

F

F

A

E

E

P

P

V

I

Q

Q

G

G

E
x
D

G

A

G

G

F

I

Y

V

P

V

A

P

P

P

P

E

D

N

L

F

V

F

R

K

A

E

I

L

R

K

Q

K

L

L

C

L

D

E

A

E

H

Y

G

G

I

I

V

L

M

L

I

V

A

M

D

D

E

E

V

V

Q

Q

T

T

G

G

F

I

A

G

R

R

T

T

G

G

T

K

L

W

F

F

A

A

M

S

H

E

G

W

Y

F

D

N

V

V

A

K

P

P

D

D

L

M

V

I

T

I

M

F

A

G

K
|
K

G

G

L

V

G

A

G

S

G

G

L

M

P

G

I

L

A

S

A

G

V

V

T

I

G

G

R

R

A

K

E

E

I

I

M

M

D

D

A

I

A

T

H

S

P

G

G

S

G

A

L

L

G

-

G

L

T

T

Y

P

G

A

N

N

P

P

L

V

G

I

I

S

A

A

H

E

A

A

V

T

L

L

D

E

V

I

I

I

E

E

N

N

L

L

C

L

A

K

R

N

A

A

T

L

E

E

L

V

G

G

S

E

R

F

L

I

K

M

Q

G

K

R

L

L

A

K

E

E

I

I

R

K

A

E

T

R

A

F

P

E

E

I

I

I

A

G

D

D

I

V

R

R

G

G

P

K

G

G

F

L

M

M

V

I

G

G

A

V

E

E

F

I

A

V

D

K

P

E

G

N

S

G

K

R

A

-

P

P

D

D

A

P

G

E

F

M

T

T

N

G

R

K

V

I

R

C

E

W

E

R

A

A

L

F

K

E

R

L

G

G

L

L

I

I

L

L

L

P

T

S

C

Y

G

G

V

M

H

F

G

G

N

N

V

V

I

I

R

R

F

I

L

T

A

P

P

P

I

L

T

V

I

L

P

T

E

K

P

E

H

V

F

A

A

E

E

K

A

A

L

L

E

E

I

I

L

I

E

E

A

R

S

A

V

I

A

K

A

D

A

T

R

L

Q

T

G

G

D251 (≠ E210) mutation to A: Loss of activity.
K308 (= K267) mutation to A: Loss of activity.

5wyfA Structure of amino acid racemase, 2.12 a (see paper)
33% identity, 94% coverage: 27:425/425 of query aligns to 32:437/446 of 5wyfA

query
sites
5wyfA

D

D

R

H

A

A

L

H

N

G

A

A

E

T

I

L

W

V

D

D

I

V

E

D

G

G

R

N

R

K

Y

Y

I

I

D

D

F

L

A

L

A

A

G

S

I
x
A

A

S

V

A

V

I

N

N

T

V

G

G

H

H

C

T

H

H

P

E

K

K

V

V

M

V

A

K

A

A

V

I

A

A

E

D

Q

Q

M

A

G

Q

R

K

F

L

T

I

H

H

T

Y

C

T

H

P

Q

A

V
x
Y

L

F

P

H

Y

H

E

V

N

P

Y

G

I

M

R

E

L

L

A

S

E

E

R

K

L

L

N

A

A

K

A

I

A

A

P

P

G

G

D

N

F

S

A

P

K

K

K

M

T

V

V

S

F

F

V

G

T

N

T

S

G
|
G

A
x
S

E

D

S

A

V

N

E

D

N

A

A

I

I

I

K

K

I

F

A

A

R

R

I

A

H

Y

T

T

G

G

R

R

S

Q

A

Y

V

I

V

V

A

S

F

Y

G

M

G

G

G

S

F
x
Y

H
|
H

G

G

R

S

T

T

F

Y

M

G

T

S

M

Q

S

T

L

L

T

S

G

G

K

S

V

S

E

L

P

N

Y

M

K

T

K

R

G

K

F

I

G

G

T

P

M

M

M

L

P

P

E

S

V

V

Y

V

H

H

V

V

P

P

F

Y

P

P

Q

D

A

S

L

Y

-

R

-

T

-

Y

-

P

-

G

-

E

-

T

-

E

H

H

G

D

I

V

S

S

T

L

E

-

E

R

A

Y

M

F

A

N

A

E

L

F

A

K

R

K

L

P

F

F

K

E

A

S

D

F

L

L

D

P

P

A

G

D

R

E

V

T

A

A

A

C

I

V

I

L

F

I

E
|
E

P

P

V

I

Q

Q

G

G

E
x
D

G

G

F

I

Y

I

P

K

A

A

P

P

P

E

D

E

L

Y

V

M

R

Q

A

L

I

V

R

Y

Q

K

L

F

C

C

D

H

A

E

H

H

G

G

I

I

V

L

M

F

I

A

A

I

D
|
D

E

E

V
|
V

Q
x
N

T

Q

G

G

F

L

A

G

R

R

T

T

G

G

T

K

L

M

F

W

A

A

M

I

H

Q

G

Q

Y

F

-

K

D

D

V

I

A

E

P

P

D

D

L

L

V

M

T

S

M

V

A

G

K
|
K

G

S

L

L

G

A

G

S

G

G

L

M

P

P

I

L

A

S

A

A

V

V

T

I

G

G

R

K

A

K

E

E

I

V

M

M

D

Q

A

S

A

L

H

D

P

A

G

P

G

A

L

H

G

L

G
x
F

T
|
T

Y

T

G

A

G

G

N

N

P

P

L

V

G

C

I

S

A

A

A

S

H

L

A

A

V

T

L

L

D

D

V

V

I

I

E

E

E

Y

E

E

N

G

L

L

C

V

A

E

R

K

A

S

T

A

E

T

L

D

G

G

S

A

R

Y

L

A

K

K

Q

Q

K

R

L

F

A

L

E

E

I

M

R

Q

A

Q

T

R

A

H

P

P

E

M

I

I

A

G

D

D

I

V

R

R

G

M

P

W

G

G

F

L

M

N

V

G

G

G

A

I

E

E

F

L

A

V

-

K

D

D

P

P

G

K

S

T

K

K

A

E

P

P

D

D

A

S

G

D

F

A

T

A

N

T

R

K

V

V

R

I

E

Y

A

A

L

F

K

A

R

H

G

G

L

V

I

V

L

I

T

T

C

L

G

A

V

-

H

-

G

G

N

N

V

I

I

L

R
|
R

F

F

L

Q

A

P

P

P

I

L

T

V

I

I

P

P

E

R

P

E

H

Q

F

L

A

D

E

Q

A

A

L

L

E

Q

I

V

L

L

E

D

A

D

S

A

V

F

A

T

A

A

A

V

R

E

Q

N

G

G

active site: Y142 (≠ F137), E217 (= E205), D250 (= D238), N253 (≠ Q241), K280 (= K267), T309 (= T296), R408 (= R396)
binding n-[o-phosphono-pyridoxyl]-isoleucine: A54 (≠ I49), Y84 (≠ V79), G115 (= G110), S116 (≠ A111), Y142 (≠ F137), H143 (= H138), D222 (≠ E210), D250 (= D238), V252 (= V240), N253 (≠ Q241), K280 (= K267), F308 (≠ G295), T309 (= T296), R408 (= R396)

Sites not aligning to the query:

active site: 22

5wyaA Structure of amino acid racemase, 2.65 a (see paper)
33% identity, 94% coverage: 27:425/425 of query aligns to 30:435/439 of 5wyaA

query
sites
5wyaA

D

D

R

H

A

A

L

H

N

G

A

A

E

T

I

L

W

V

D

D

I

V

E

D

G

G

R

N

R

K

Y

Y

I

I

D

D

F

L

A

L

A

A

G

S

I
x
A

A

S

V

A

V

I

N

N

T

V

G

G

H

H

C

T

H

H

P

E

K

K

V

V

M

V

A

K

A

A

V

I

A

A

E

D

Q

Q

M

A

G

Q

R

K

F

L

T

I

H

H

T

Y

C

T

H

P

Q

A

V
x
Y

L

F

P

H

Y

H

E

V

N

P

Y

G

I

M

R

E

L

L

A

S

E

E

R

K

L

L

N

A

A

K

A

I

A

A

P

P

G

G

D

N

F

S

A

P

K

K

K

M

T

V

V

S

F

F

V

G

T

N

T
x
S

G
|
G

A
x
S

E

D

S

A

V

N

E

D

N

A

A

I

I

I

K

K

I

F

A

A

R

R

I

A

H

Y

T

T

G

G

R

R

S

Q

A

Y

V

I

V

V

A

S

F

Y

G

M

G

G

G

S

F
x
Y

H
|
H

G

G

R

S

T

T

F

Y

M

G

T

S

M

Q

S

T

L

L

T

S

G

G

K

S

V

S

E

L

P

N

Y

M

K

T

K

R

G

K

F

I

G

G

T

P

M

M

M

L

P

P

E

S

V

V

Y

V

H

H

V

V

P

P

F

Y

P

P

Q

D

A

S

L

Y

-

R

-

T

-

Y

-

P

-

G

-

E

-

T

-

E

H

H

G

D

I

V

S

S

T

L

E

-

E

R

A

Y

M

F

A

N

A

E

L

F

A

K

R

K

L

P

F

F

K

E

A

S

D

F

L

L

D

P

P

A

G

D

R

E

V

T

A

A

A

C

I

V

I

L

F

I

E
|
E

P

P

V

I

Q

Q

G

G

E

D

G

G

F

I

Y

I

P

K

A

A

P

P

P

E

D

E

L

Y

V

M

R

Q

A

L

I

V

R

Y

Q

K

L

F

C

C

D

H

A

E

H

H

G

G

I

I

V

L

M

F

I

A

A

I

D
|
D

E

E

V
|
V

Q
x
N

T

Q

G

G

F

L

A

G

R

R

T

T

G

G

T

K

L

M

F

W

A

A

M

I

H

Q

G

Q

Y

F

-

K

D

D

V

I

A

E

P

P

D

D

L

L

V

M

T

S

M

V

A

G

K
|
K

G

S

L

L

G

A

G

S

G

G

L

M

P

P

I

L

A

S

A

A

V

V

T

I

G

G

R

K

A

K

E

E

I

V

M

M

D

Q

A

S