SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011840919.1 NCBI__GCF_000015985.1:WP_011840919.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q8DLI5 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1) (see paper)
39% identity, 97% coverage: 1:269/277 of query aligns to 1:270/485 of Q8DLI5

query
sites
Q8DLI5

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R6 (= R7) binding L-glutamate
Y192 (= Y192) binding L-glutamate

2cfoA Non-discriminating glutamyl-tRNA synthetase from thermosynechococcus elongatus in complex with glu (see paper)
38% identity, 95% coverage: 6:269/277 of query aligns to 4:269/484 of 2cfoA

query
sites
2cfoA

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active site: S9 (= S11), L239 (= L240), K250 (= K251)
binding glutamic acid: R5 (= R7), Y191 (= Y192), R209 (= R210), H213 (≠ L214)

P27305 Glutamyl-Q tRNA(Asp) synthetase; Glu-Q-RSs; EC 6.1.1.- from Escherichia coli (strain K12) (see paper)
40% identity, 92% coverage: 5:260/277 of query aligns to 17:250/308 of P27305

query
sites
P27305

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E55 (= E41) binding L-glutamate
Y182 (= Y192) binding L-glutamate
R200 (= R210) binding L-glutamate

4a91A Crystal structure of the glutamyl-queuosine trnaasp synthetase from e. Coli complexed with l-glutamate (see paper)
40% identity, 90% coverage: 5:252/277 of query aligns to 5:230/290 of 4a91A

query
sites
4a91A

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active site: S11 (= S11), K229 (= K251)
binding glutamic acid: R7 (= R7), A9 (= A9), S11 (= S11), E43 (= E41), Y170 (= Y192), R188 (= R210), L192 (= L214)
binding zinc ion: C99 (= C97), C101 (= C99), Y113 (= Y124), C117 (= C128)

2cv2A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu) and an enzyme inhibitor, glu-ams (see paper)
33% identity, 99% coverage: 5:277/277 of query aligns to 3:275/468 of 2cv2A

query
sites
2cv2A

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R

V

F

V

H
x
Y

E

D

T

N

G

-

P

-

E

-

A

-

E

-

G

-

W

-

H

-

A

-

P

-

Q

-

D

-

L

-

A

-

R

Q

S
x
E

V

I

G

P

D

D

V

V

V
|
V

L

L

A

L

R
x
K

R
x
S

G

D

F

G

G

Y

T

P

S

T

Y
|
Y

H

H

L

L

A

A

V

N

V

V

D

D

A

H

A

L

Q

M

G

G

V

V

T

T

E

D

V

V

V

I

R
|
R

G
x
A

A
x
E

D
x
E

L
x
W

F

L

E
x
V

A

S

T

T

A

P

I

I

H

H

V

V

L

L

Q

Y

R

R

L

A

L

F

G

G

L

W

P

E

V

A

P

P

D

R

F

F

H

Y

H

H

H

M

R

P

L
|
L

I
x
L

R
|
R

D

N

A

P

A

D

G
x
K

K

T

R

K

L

I

A

S

K
|
K

R

R

D

K

D

S

S

H

K

T

A

S

L

L

A

D

K

W

Y

Y

R

K

A

A

E

E

G

G

M

F

S

L

P

P

A

E

E

A

V

L

R

R

-

N

-

Y

-

L

A

C

L
|
L

V
x
M

G
|
G

F

F

active site: K246 (= K251)
binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R5 (= R7), A7 (= A9), S9 (= S11), G17 (= G19), I21 (≠ S23), E41 (= E41), Y187 (= Y192), R205 (= R210), A206 (≠ G211), E208 (≠ D213), W209 (≠ L214), L235 (= L240), L236 (≠ I241)
binding : S9 (= S11), T43 (≠ I43), D44 (= D44), R47 (= R47), V145 (≠ A141), R163 (≠ G154), Y168 (≠ H159), E172 (≠ S177), V177 (= V182), K180 (≠ R185), S181 (≠ R186), Y187 (= Y192), E207 (≠ A212), E208 (≠ D213), W209 (≠ L214), V211 (≠ E216), R237 (= R242), K241 (≠ G246), L272 (= L274), M273 (≠ V275), G274 (= G276)

Sites not aligning to the query:

binding : 282, 299, 303, 304, 309, 312, 319, 357, 358, 417, 432, 435, 442, 443, 444, 446, 447, 448

2cv1A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu), atp, and an analog of l-glutamate: a quaternary complex
33% identity, 99% coverage: 5:277/277 of query aligns to 3:275/468 of 2cv1A

query
sites
2cv1A

V

V

T

T

R
|
R

F

I

A
|
A

P
|
P

S
|
S

P

P

T

T

G

G

P

D

L

P

H

H

L

V

G
|
G

H
x
T

A

A

F

Y

S
x
I

A

A

L

L

-

F

-

N

T

Y

V

A

W

W

Q

A

R

R

A

R

A

N

G

G

T

R

A

F

L

I

L

V

R

R

I

I

E
|
E

D

D

I
x
T

D
|
D

R

R

S

A

R
|
R

C

Y

R

V

P

P

E

G

W

A

E

E

A

E

Q

R

I

I

F

L

N

A

D

A

L

L

H

K

W

W

L

L

G

G

L

L

A

S

W

Y

-

D

-

E

-

G

-

P

-

D

-

V

-

G

P

P

E

H

P

G

V

P

L

Y

R

R

Q

Q

S

S

D

E

R

R

G

L

G

P

A

L

Y

Y

R

Q

A

K

A

Y

L

A

D

E

R

E

L

L

T

L

A

K

M

R

G

G

L

W

T

A

Y

Y

P

-

C

-

R

-

C

-

S

-

R

-

A

-

D

-

I

-

R

-

A

R

A

A

L

F

A

E

A

T

P

P

Q

E

E

E

G

L

V

E

P

Q

V

I

G

R

P

K

D

E

G

K

I

G

V

G

Y

Y

P

D

G

G

T

R

C

A

R

R

G

N

R

I

P

P

M

P

A

E

E

E

A

A

-

E

-

R

-

A

-

R

G

G

P

E

D

P

D

H

A
x
V

I

I

R

R

L

L

D

K

M

V

A

P

R

R

-

P

-

G

-

T

-

E

A

V

E

K

E

D

H

E

A

L

G
x
R

G

G

L

V

R
x
V

F

V

H

Y

E

D

T

N

G

-

P

-

E

-

A

-

E

-

G

-

W

-

H

-

A

-

P

-

Q

-

D

-

L

-

A

-

R

Q

S
x
E

V

I

G

P

D

D

V

V

V
|
V

L

L

A

L

R
x
K

R
x
S

G

D

F

G

G

Y

T

P

S

T

Y
|
Y

H

H

L

L

A

A

V

N

V

V

D

D

A

H

A

L

Q

M

G

G

V

V

T

T

E

D

V

V

V

I

R
|
R

G
x
A

A
x
E

D
x
E

L
x
W

F

L

E
x
V

A

S

T

T

A

P

I

I

H

H

V

V

L

L

Q

Y

R

R

L

A

L

F

G

G

L

W

P

E

V

A

P

P

D

R

F

F

H

Y

H

H

H

M

R

P

L
|
L

I
x
L

R
|
R

D

N

A

P

A

D

G
x
K

K

T

R
x
K

L

I

A

S

K
|
K

R

R

D

K

D

S

S

H

K

T

A

S

L

L

A

D

K

W

Y

Y

R

K

A

A

E

E

G

G

M

F

S

L

P

P

A

E

E

A

V

L

R

R

-

N

-

Y

-

L

A

C

L

L

V
x
M

G
|
G

F

F

active site: K246 (= K251)
binding adenosine-5'-triphosphate: P8 (= P10), S9 (= S11), G17 (= G19), T18 (≠ H20), I21 (≠ S23), R47 (= R47), A206 (≠ G211), W209 (≠ L214), L235 (= L240), L236 (≠ I241)
binding (4s)-4-amino-5-hydroxypentanoic acid: R5 (= R7), A7 (= A9), E41 (= E41), Y187 (= Y192), R205 (= R210), W209 (≠ L214)
binding : S9 (= S11), E41 (= E41), T43 (≠ I43), D44 (= D44), R47 (= R47), V145 (≠ A141), R163 (≠ G154), V166 (≠ R157), E172 (≠ S177), V177 (= V182), K180 (≠ R185), S181 (≠ R186), Y187 (= Y192), E207 (≠ A212), E208 (≠ D213), W209 (≠ L214), V211 (≠ E216), R237 (= R242), K241 (≠ G246), K243 (≠ R248), M273 (≠ V275), G274 (= G276)

Sites not aligning to the query:

binding : 276, 282, 299, 303, 304, 309, 312, 319, 357, 358, 417, 427, 432, 435, 442, 443, 444, 446, 447, 448

2cuzA Glutamyl-tRNA synthetase from thermus thermophilus in complex with l- glutamate (see paper)
33% identity, 99% coverage: 5:277/277 of query aligns to 3:275/468 of 2cuzA

query
sites
2cuzA

V

V

T

T

R
|
R

F

I

A
|
A

P

P

S
|
S

P

P

T

T

G

G

P

D

L

P

H

H

L

V

G

G

H

T

A

A

F

Y

S

I

A

A

L

L

-

F

-

N

T

Y

V

A

W

W

Q

A

R

R

A

R

A

N

G

G

T

R

A

F

L

I

L

V

R

R

I

I

E
|
E

D

D

I

T

D

D

R

R

S

A

R

R

C

Y

R

V

P

P

E

G

W

A

E

E

A

E

Q

R

I

I

F

L

N

A

D

A

L

L

H

K

W

W

L

L

G

G

L

L

A

S

W

Y

-

D

-

E

-

G

-

P

-

D

-

V

-

G

P

P

E

H

P

G

V

P

L

Y

R

R

Q

Q

S

S

D

E

R

R

G

L

G

P

A

L

Y

Y

R

Q

A

K

A

Y

L

A

D

E

R

E

L

L

T

L

A

K

M

R

G

G

L

W

T

A

Y

Y

P

-

C

-

R

-

C

-

S

-

R

-

A

-

D

-

I

-

R

-

A

R

A

A

L

F

A

E

A

T

P

P

Q

E

E

E

G

L

V

E

P

Q

V

I

G

R

P

K

D

E

G

K

I

G

V

G

Y

Y

P

D

G

G

T

R

C

A

R

R

G

N

R

I

P

P

M

P

A

E

E

E

A

A

-

E

-

R

-

A

-

R

G

G

P

E

D

P

D

H

A

V

I

I

R

R

L

L

D

K

M

V

A

P

R

R

-

P

-

G

-

T

-

E

A

V

E

K

E

D

H

E

A

L

G

R

G

G

L

V

R

V

F

V

H

Y

E

D

T

N

G

-

P

-

E

-

A

-

E

-

G

-

W

-

H

-

A

-

P

-

Q

-

D

-

L

-

A

-

R

Q

S

E

V

I

G

P

D

D

V

V

L

L

A

L

R

K

R

S

G

D

F

G

G

Y

T

P

S

T

Y
|
Y

H

H

L

L

A

A

V

N

V

V

D

D

A

H

A

L

Q

M

G

G

V

V

T

T

E

D

V

V

V

I

R
|
R

G

A

A

E

D

E

L

W

F

L

E

V

A

S

T

T

A

P

I

I

H

H

V

V

L

L

Q

Y

R

R

L

A

L

F

G

G

L

W

P

E

V

A

P

P

D

R

F

F

H

Y

H

H

H

M

R

P

L

L

I

L

R

R

D

N

A

P

A

D

G

K

K

T

R

K

L

I

A

S

K
|
K

R

R

D

K

D

S

S

H

K

T

A

S

L

L

A

D

K

W

Y

Y

R

K

A

A

E

E

G

G

M

F

S

L

P

P

A

E

E

A

V

L

R

R

-

N

-

Y

-

L

A

C

L

L

V

M

G

G

F

F

active site: K246 (= K251)
binding glutamic acid: R5 (= R7), A7 (= A9), S9 (= S11), E41 (= E41), Y187 (= Y192), R205 (= R210)

1n78A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with tRNA(glu) and glutamol-amp. (see paper)
33% identity, 99% coverage: 5:277/277 of query aligns to 3:275/468 of 1n78A

query
sites
1n78A

V

V

T

T

R
|
R

F

I

A
|
A

P
|
P

S
|
S

P

P

T

T

G

G

P

D

L

P

H

H

L

V

G
|
G

H
x
T

A

A

F

Y

S
x
I

A

A

L

L

-

F

-

N

T

Y

V

A

W

W

Q

A

R

R

A

R

A

N

G

G

T

R

A

F

L

I

L

V

R

R

I

I

E
|
E

D

D

I
x
T

D
|
D

R

R

S

A

R
|
R

C

Y

R

V

P

P

E

G

W

A

E

E

A

E

Q

R

I

I

F

L

N

A

D

A

L

L

H

K

W

W

L

L

G

G

L

L

A

S

W

Y

-

D

-

E

-

G

-

P

-

D

-

V

-

G

P

P

E

H

P

G

V

P

L

Y

R

R

Q

Q

S

S

D

E

R

R

G

L

G

P

A

L

Y

Y

R

Q

A

K

A

Y

L

A

D

E

R

E

L

L

T

L

A

K

M

R

G

G

L

W

T

A

Y

Y

P

-

C

-

R

-

C

-

S

-

R

-

A

-

D

-

I

-

R

-

A

R

A

A

L

F

A

E

A

T

P

P

Q

E

E

E

G

L

V

E

P

Q

V

I

G

R

P

K

D

E

G

K

I

G

V

G

Y

Y

P

D

G

G

T

R

C

A

R

R

G

N

R

I

P

P

M

P

A

E

E

E

A

A

-

E

-

R

-

A

-

R

G

G

P

E

D

P

D

H

A
x
V

I

I

R

R

L

L

D

K

M

V

A

P

R

R

-

P

-

G

-

T

-

E

A

V

E

K

E

D

H

E

A

L

G
x
R

G

G

L

V

R
x
V

F

V

H
x
Y

E

D

T

N

G

-

P

-

E

-

A

-

E

-

G

-

W

-

H

-

A

-

P

-

Q

-

D

-

L

-

A

-

R

Q

S
x
E

V

I

G

P

D

D

V

V

V
|
V

L

L

A

L

R
x
K

R
x
S

G

D

F

G

G

Y

T

P

S

T

Y
|
Y

H

H

L

L

A

A

V
x
N

V

V

D

D

A

H

A

L

Q

M

G

G

V

V

T

T

E

D

V

V

V

I

R
|
R

G
x
A

A
x
E

D
x
E

L
x
W

F
x
L

E
x
V

A

S

T

T

A

P

I

I

H

H

V

V

L

L

Q

Y

R

R

L

A

L

F

G

G

L

W

P

E

V

A

P

P

D

R

F

F

H

Y

H

H

H

M

R

P

L
|
L

I
x
L

R
|
R

D

N

A

P

A

D

G
x
K

K

T

R

K

L

I

A

S

K
|
K

R

R

D

K

D

S

S

H

K

T

A

S

L

L

A

D

K

W

Y

Y

R

K

A

A

E

E

G

G

M

F

S

L

P

P

A

E

E

A

V

L

R

R

-

N

-

Y

-

L

A

C

L

L

V
x
M

G
|
G

F

F

active site: K246 (= K251)
binding glutamol-amp: R5 (= R7), A7 (= A9), P8 (= P10), S9 (= S11), G17 (= G19), T18 (≠ H20), I21 (≠ S23), E41 (= E41), Y187 (= Y192), N191 (≠ V196), R205 (= R210), A206 (≠ G211), E208 (≠ D213), W209 (≠ L214), L235 (= L240), L236 (≠ I241)
binding : S9 (= S11), T43 (≠ I43), D44 (= D44), R47 (= R47), V145 (≠ A141), R163 (≠ G154), V166 (≠ R157), Y168 (≠ H159), E172 (≠ S177), V177 (= V182), K180 (≠ R185), S181 (≠ R186), Y187 (= Y192), E207 (≠ A212), E208 (≠ D213), W209 (≠ L214), L210 (≠ F215), V211 (≠ E216), R237 (= R242), K241 (≠ G246), M273 (≠ V275), G274 (= G276)

Sites not aligning to the query:

binding : 282, 297, 303, 304, 309, 312, 319, 357, 358, 417, 427, 432, 435, 442, 443, 444, 446, 447, 448

1j09A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with atp and glu (see paper)
33% identity, 99% coverage: 5:277/277 of query aligns to 3:275/468 of 1j09A

query
sites
1j09A

V

V

T

T

R
|
R

F

I

A
|
A

P

P

S
|
S

P

P

T

T

G

G

P

D

L

P

H
|
H

L

V

G

G

H

T

A

A

F

Y

S

I

A

A

L

L

-

F

-

N

T

Y

V

A

W

W

Q

A

R

R

A

R

A

N

G

G

T

R

A

F

L

I

L

V

R

R

I

I

E
|
E

D

D

I

T

D

D

R

R

S

A

R

R

C

Y

R

V

P

P

E

G

W

A

E

E

A

E

Q

R

I

I

F

L

N

A

D

A

L

L

H

K

W

W

L

L

G

G

L

L

A

S

W

Y

-

D

-

E

-

G

-

P

-

D

-

V

-

G

P

P

E

H

P

G

V

P

L

Y

R

R

Q

Q

S

S

D

E

R

R

G

L

G

P

A

L

Y

Y

R

Q

A

K

A

Y

L

A

D

E

R

E

L

L

T

L

A

K

M

R

G

G

L

W

T

A

Y

Y

P

-

C

-

R

-

C

-

S

-

R

-

A

-

D

-

I

-

R

-

A

R

A

A

L

F

A

E

A

T

P

P

Q

E

E

E

G

L

V

E

P

Q

V

I

G

R

P

K

D

E

G

K

I

G

V

G

Y

Y

P

D

G

G

T

R

C

A

R

R

G

N

R

I

P

P

M

P

A

E

E

E

A

A

-

E

-

R

-

A

-

R

G

G

P

E

D

P

D

H

A

V

I

I

R

R

L

L

D

K

M

V

A

P

R

R

-

P

-

G

-

T

-

E

A

V

E

K

E

D

H

E

A

L

G

R

G

G

L

V

R

V

F

V

H

Y

E

D

T

N

G

-

P

-

E

-

A

-

E

-

G

-

W

-

H

-

A

-

P

-

Q

-

D

-

L

-

A

-

R

Q

S

E

V

I

G

P

D

D

V

V

L

L

A

L

R

K

R

S

G

D

F

G

G

Y

T

P

S

T

Y
|
Y

H

H

L

L

A

A

V
x
N

V

V

D

D

A

H

A

L

Q

M

G

G

V

V

T

T

E

D

V

V

V

I

R
|
R

G

A

A

E

D
x
E

L
x
W

F

L

E

V

A

S

T

T

A

P

I

I

H

H

V

V

L

L

Q

Y

R

R

L

A

L

F

G

G

L

W

P

E

V

A

P

P

D

R

F

F

H

Y

H

H

H

M

R

P

L
|
L

I
x
L

R

R

D

N

A

P

A

D

G

K

K

T

R
x
K

L
x
I

A
x
S

K
|
K

R
|
R

D

K

D

S

S

H

K

T

A

S

L

L

A

D

K

W

Y

Y

R

K

A

A

E

E

G

G

M

F

S

L

P

P

A

E

E

A

V

L

R

R

-

N

-

Y

-

L

A

C

L

L

V

M

G

G

F

F

active site: K246 (= K251)
binding adenosine-5'-triphosphate: H15 (= H17), E208 (≠ D213), L235 (= L240), L236 (≠ I241), K243 (≠ R248), I244 (≠ L249), S245 (≠ A250), K246 (= K251), R247 (= R252)
binding glutamic acid: R5 (= R7), A7 (= A9), S9 (= S11), E41 (= E41), Y187 (= Y192), N191 (≠ V196), R205 (= R210), W209 (≠ L214)

P27000 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
33% identity, 99% coverage: 5:277/277 of query aligns to 3:275/468 of P27000

query
sites
P27000

V

V

T

T

R

R

F

I

A

A

P

P

S

S

P

P

T

T

G

G

P

D

L

P

H

H

L

V

G

G

H

T

A

A

F

Y

S

I

A

A

L

L

-

F

-

N

T

Y

V

A

W

W

Q

A

R

R

A

R

A

N

G

G

T

R

A

F

L

I

L

V

R

R

I

I

E

E

D

D

I

T

D

D

R

R

S

A

R

R

C

Y

R

V

P

P

E

G

W

A

E

E

A

E

Q

R

I

I

F

L

N

A

D

A

L

L

H

K

W

W

L

L

G

G

L

L

A

S

W

Y

-

D

-

E

-

G

-

P

-

D

-

V

-

G

P

P

E

H

P

G

V

P

L

Y

R

R

Q

Q

S

S

D

E

R

R

G

L

G

P

A

L

Y

Y

R

Q

A

K

A

Y

L

A

D

E

R

E

L

L

T

L

A

K

M

R

G

G

L

W

T

A

Y

Y

P

-

C

-

R

-

C

-

S

-

R

-

A

-

D

-

I

-

R

-

A

R

A

A

L

F

A

E

A

T

P

P

Q

E

E

E

G

L

V

E

P

Q

V

I

G

R

P

K

D

E

G

K

I

G

V

G

Y

Y

P

D

G

G

T

R

C

A

R

R

G

N

R

I

P

P

M

P

A

E

E

E

A

A

-

E

-

R

-

A

-

R

G

G

P

E

D

P

D

H

A

V

I

I

R

R

L

L

D

K

M

V

A

P

R

R

-

P

-

G

-

T

-

E

A

V

E

K

E

D

H

E

A

L

G

R

G

G

L

V

R

V

F

V

H

Y

E

D

T

N

G

-

P

-

E

-

A

-

E

-

G

-

W

-

H

-

A

-

P

-

Q

-

D

-

L

-

A

-

R

Q

S

E

V

I

G

P

D

D

V

V

L

L

A

L

R

K

R

S

G

D

F

G

G

Y

T

P

S

T

Y

Y

H

H

L

L

A

A

V

N

V

V

D

D

A

H

A

L

Q

M

G

G

V

V

T

T

E

D

V

V

V

I

R

R

G

A

A

E

D

E

L

W

F

L

E

V

A

S

T

T

A

P

I

I

H

H

V

V

L

L

Q

Y

R

R

L

A

L

F

G

G

L

W

P

E

V

A

P

P

D

R

F

F

H

Y

H

H

H

M

R

P

L

L

I

L

R

R

D

N

A

P

A

D

G

K

K

T

R

K

L

I

A

S

K

K

R

R

D

K

D

S

S

H

K

T

A

S

L

L

A

D

K

W

Y

Y

R

K

A

A

E

E

G

G

M

F

S

L

P

P

A

E

E

A

V

L

R

R

-

N

-

Y

-

L

A

C

L

L

V

M

G

G

F

F

Sites not aligning to the query:

358 R→Q: Reduces affinity for tRNA and abolishes the ability to discriminate between tRNA(Glu) and tRNA(Gln).

1g59A Glutamyl-tRNA synthetase complexed with tRNA(glu). (see paper)
33% identity, 99% coverage: 5:277/277 of query aligns to 3:275/468 of 1g59A

query
sites
1g59A

V

V

T

T

R

R

F

I

A

A

P

P

S

S

P

P

T

T

G

G

P

D

L

P

H

H

L

V

G

G

H

T

A

A

F

Y

S

I

A

A

L

L

-

F

-

N

T

Y

V

A

W

W

Q

A

R

R

A

R

A

N

G

G

T

R

A

F

L

I

L

V

R

R

I

I

E

E

D

D

I

T

D
|
D

R
|
R

S
x
A

R
|
R

C

Y

R

V

P

P

E

G

W

A

E

E

A

E

Q

R

I

I

F

L

N

A

D

A

L

L

H

K

W

W

L

L

G

G

L

L

A

S

W

Y

-

D

-

E

-

G

-

P

-

D

-

V

-

A

P

P

E

T

P

G

V

P

L

Y

R

R

Q

Q

S

S

D

E

R

R

G

L

G

P

A

L

Y

Y

R

Q

A

K

A

Y

L

A

D

E

R

E

L

L

T

L

A

K

M

R

G

G

L

W

T

A

Y

Y

P

-

C

-

R

-

C

-

S

-

R

-

A

-

D

-

I

-

R

-

A

R

A

A

L

F

A

E

A

T

P
|
P

Q

E

E

E

G

L

V

E

P

Q

V

I

G

R

P

K

D

E

G

K

I

G

V

G

Y

Y

P

D

G

G

T

R

C

A

R

R

G

N

R

I

P

P

M

P

A

E

E

E

A

A

-

E

-

R

-

A

-

R

G

G

P

E

D

P

D

H

A
x
V

I

I

R

R

L

L

D

K

M

V

A

P

R

R

-

P

-

G

-

T

-

E

A

V

E

K

E

D

H

E

A

L

G
x
R

G

G

L

V

R
x
V

F

V

H

Y

E

D

T

N

G

-

P

-

E

-

A

-

E

-

G

-

W

-

H

-

A

-

P

-

Q

-

D

-

L

-

A

-

R

Q

S
x
E

V

I

G

P

D

D

V

V

V
|
V

L

L

A

L

R
x
K

R
x
S

G
x
D

F

G

G

Y

T

P

S

T

Y

Y

H

H

L

L

A

A

V

N

V

V

D

D

A

H

A

L

Q

M

G

G

V

V

T

T

E

D

V

V

V

I

R

R

G

A

A
x
E

D
x
E

L

W

F

L

E

V

A

S

T

T

A

P

I

I

H

H

V

V

L

L

Q

Y

R

R

L

A

L

F

G

G

L

W

P

E

V

A

P

P

D

R

F

F

H

Y

H

H

H

M

R

P

L

L

I

L

R
|
R

D

N

A

P

A

D

G
x
K

K
x
T

R
x
K

L

I

A

S

K

K

R

R

D

K

D

S

S

H

K

T

A

S

L

L

A

D

K

W

Y

Y

R

K

A

A

E

E

G

G

M

F

S

L

P

P

A

E

E

A

V

L

R

R

-

N

-

Y

-

L

A

C

L

L

V
x
M

G
|
G

F

F

binding : D44 (= D44), R45 (= R45), A46 (≠ S46), R47 (= R47), P109 (= P111), V145 (≠ A141), R163 (≠ G154), V166 (≠ R157), E172 (≠ S177), V177 (= V182), K180 (≠ R185), S181 (≠ R186), D182 (≠ G187), E207 (≠ A212), E208 (≠ D213), R237 (= R242), K241 (≠ G246), T242 (≠ K247), K243 (≠ R248), M273 (≠ V275), G274 (= G276)

Sites not aligning to the query:

binding : 282, 299, 300, 303, 304, 309, 312, 319, 357, 358, 417, 426, 427, 432, 435, 442, 443, 444, 445, 446, 447, 448

P04805 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Escherichia coli (strain K12) (see 4 papers)
34% identity, 95% coverage: 6:268/277 of query aligns to 5:257/471 of P04805

query
sites
P04805

T

T

R

R

F

F

A

A

P

P

S

S

P

P

T

T

G

G

P

Y

L

L

H

H

L

V

G

G

H

G

A

A

F

R

S

T

A

A

L

L

T

Y

V

S

W

W

-

L

-

F

Q

A

R

R

A

N

A

H

G

G

T

E

A

F

L

V

L

L

R

R

I

I

E

E

D

D

I

T

D

D

R

L

S

E

R

R

C

S

R

T

P

P

E

E

W

A

E

I

A

E

Q

A

I

I

F

M

N

D

D

G

L

M

H

N

W

W

L

L

G

S

L

L

A

E

W

W

P

D

E

E

P

G

V

P

L

Y

R

Y

Q

Q

S

T

D

K

R

R

G

F

G

D

A

R

Y

Y

R

N

A

A

A

V

L

I

D

D

R

Q

L

M

T

L

A

E

M

E

G

G

L

T

T

A

Y

Y

P

K

C
|
C

R

Y

C
|
C

S

S

R

K

A

E

D

R

I

L

R

E

A

-

A

A

L

L

A

R

A

E

P

E

Q

Q

E

M

G

A

V

K

P

G

V

E

G

K

P

P

D

R

G

-

I

-

V

-

Y

Y

P

D

G

G

T

R

C
|
C

R

R

G
x
H

R

S

P
x
H

M

E

A
x
H

E
x
H

A

A

G

D

P

-

D

D

D

E

A

P

I
x
C

R

V

L

V

D

R

M

F

A

A

R

N

A

P

E

Q

E

E

H

-

A

-

G

G

G

S

L

V

R

V

F

F

H

D

E

D

T

Q

G

-

P

-

E

-

A

-

E

-

G

-

W

I

H

R

A

G

P

P

Q

I

D

E

L

F

A

S

R

N

S

Q

V

E

G

L

D

D

V

D

V

L

L

I

A

I

R

R

G

T

F

D

G

G

T

S

-

P

S

T

Y

Y

H

N

L

F

A

C

V

V

D

D

A

W

A

D

Q

M

G

E

V

I

T

T

E

H

V

V

V

I

R

R

G

G

A

E

D

D

L

H

F

I

E

N

A

N

T

T

A

P

I

R

H

Q

V

I

L

N

L

I

Q

L

R

K

L

A

L

L

G

K

L

A

P

P

V

V

P

P

D

V

F

Y

H

A

H

H

H

V

R

S

L

M

I

I

R

N

D

G

A

D

G

G

K

K

R

K

L

L

A
x
S

K

K

R

R

D

H

D

G

S

A

K

V

A

S

L

V

A

M

K

Q

Y

Y

R

R

A

D

E

D

G

G

M

Y

S

L

P

P

C98 (= C97) mutation to S: 10-fold decrease in activity. Strong decrease in zinc content.
C100 (= C99) mutation to S: Loss of activity. Strong decrease in zinc content.; mutation to Y: Does not prevent zinc binding. Reduces only 2-fold the binding affinity for tRNA(Glu), but reduces more than 10-fold the affinity for glutamate in the presence of tRNA(Glu).
C125 (= C128) mutation to S: Loss of activity. Strong decrease in zinc content.
H127 (≠ G130) mutation to Q: 10-fold decrease in activity. Strong decrease in zinc content.
H129 (≠ P132) mutation to Q: No change in activity or in zinc content.
H131 (≠ A134) mutation to Q: No change in activity or in zinc content.
H132 (≠ E135) mutation to Q: No change in activity or in zinc content.
C138 (≠ I142) mutation to S: No change in activity or in zinc content.
S239 (≠ A250) modified: Phosphoserine; mutation to D: Does not aminoacylate tRNA(Glu), not phosphorylated by HipA.

8i9iA Glutamyl-tRNA synthetase from escherichia coli bound to glutamate and zinc
33% identity, 95% coverage: 6:268/277 of query aligns to 5:257/468 of 8i9iA

query
sites
8i9iA

T

T

R
|
R

F

F

A
|
A

P
|
P

S
|
S

P

P

T

T

G

G

P

Y

L

L

H

H

L

V

G

G

H

G

A

A

F

R

S

T

A

A

L

L

T

Y

V

S

W

W

-

L

-

F

Q

A

R

R

A

N

A

H

G

G

T

E

A

F

L

V

L

L

R

R

I

I

E

E

D

D

I

T

D

D

R

L

S

E

R

R

C

S

R

T

P

P

E

E

W

A

E

I

A

E

Q

A

I

I

F

M

N

D

D

G

L

M

H

N

W

W

L

L

G

S

L

L

A

E

W

W

P

D

E

E

P

G

V

P

L

Y

R

Y

Q

Q

S

T

D

K

R

R

G

F

G

D

A

R

Y

Y

R

N

A

A

A

V

L

I

D

D

R

Q

L

M

T

L

A

E

M

E

G

G

L

T

T

A

Y

Y

P

K

C

C

R

Y

C
|
C

S

S

R

K

A

E

D

R

I

L

R

E

A

-

A

A

L

L

A

R

A

E

P

E

Q

Q

E

M

G

A

V

K

P

G

V

E

G

K

P

P

D

R

G

-

I

-

V

-

Y
|
Y

P

D

G

G

T

R

C
|
C

R

R

G

H

R

S

P

H

M

E

A

H

E

H

A

A

G

D

P

-

D

D

D

E

A

P

I

C

R

V

L

V

D

R

M

F

A

A

R

N

A

P

E

Q

E

E

H

-

A

-

G

G

G

S

L

V

R

V

F

F

H

D

E

D

T

Q

G

-

P

-

E

-

A

-

E

-

G

-

W

I

H

R

A

G

P

P

Q

I

D

E

L

F

A

S

R

N

S

Q

V

E

G

L

D

D

V

D

V

L

L

I

A

I

R

R

G

T

F

D

G

G

T

S

-

P

S

T

Y

Y

H

N

L

F

A

C

V

V

D

D

A

W

A

D

Q

M

G

E

V

I

T

T

E

H

V

V

V

I

R
|
R

G

G

A

E

D

D

L
x
H

F

I

E

N

A

N

T

T

A

P

I

R

H

Q

V

I

L

N

L

I

Q

L

R

K

L

A

L

L

G

K

L

A

P

P

V

V

P

P

D

V

F

Y

H

A

H

H

H

V

R

S

L

M

I

I

R

N

D

G

A

D

G

G

K

E

R

K

L

L

A

S

K

K

R

R

D

H

D

G

S

A

K

V

A

S

L

V

A

M

K

Q

Y

Y

R

R

A

D

E

D

G

G

M

Y

S

L

P

P

binding glutamic acid: R6 (= R7), A8 (= A9), P9 (= P10), S10 (= S11), R199 (= R210), H203 (≠ L214)
binding zinc ion: C100 (= C99), Y121 (= Y124), C125 (= C128)

4g6zA Crystal structure of a glutamyl-tRNA synthetase glurs from burkholderia thailandensis bound to l-glutamate (see paper)
32% identity, 96% coverage: 6:271/277 of query aligns to 5:245/380 of 4g6zA

query
sites
4g6zA

T

T

R
|
R

F

F

A
|
A

P

P

S
|
S

P

P

T

T

G

G

P

F

L

I

H

H

L

L

G

G

H

N

A

I

F

R

S

S

A

A

L

L

T

Y

V

P

W

W

Q

A

-

F

-

A

R

R

A

K

A

M

G

K

G

G

T

T

A

F

L

V

L

L

R

R

I

I

E
|
E

D

D

I

T

D

D

R

V

S

E

R

R

C

S

R

S

P

Q

E

E

W

A

E

V

A

D

Q

A

I

I

F

L

N

E

D

G

L

M

H

A

W

W

L

L

G

G

L

L

A

D

W

Y

P

D

E

E

P

G

V

P

L

Y

R

Y

Q

Q

S

M

D

Q

R

R

G

M

G

D

A

R

Y

Y

R

R

A

E

A

V

L

L

D

A

R

Q

L

M

T

Q

A

E

M

K

G

G

L

L

T

V

Y

Y

P

P

C

C

R

Y

C

M

S

-

R

R

A

Y

D

D

I

-

R

G

A

T

A

W

L

R

A

P

A

E

P

P

Q

G

E

K

G

V

V

L

P

P

V

E

G

P

P

P

D

A

G

G

I

V

V

A

Y

P

P

V

G

L

T

R

C

F

R

R

G

N

R

P

P

L

M

T

A

G

E

T

A

V

G

A

P

W

D

D

A

A

I

V

R

K

L

G

D

R

M

V

A

E

R

I

A

S

E

N

E

E

H

-

A

-

G

-

L

-

R

-

F

-

H

-

E

-

T

-

G

-

P

-

E

-

A

-

E

-

G

-

W

-

H

-

A

-

P

-

Q

-

D

-

L

-

A

-

R

-

S

E

V

L

G

D

D

D

V

L

V

V

L

V

A

A

R

R

R

P

G

D

F

G

G

T

T

P

S

M

Y

Y

H

N

L

F

A

C

V

V

D

D

A

L

A

D

Q

M

G

G

V

I

T

T

E

H

V

V

V

I

R
|
R

G

G

A

D

D

D

L
x
H

F

V

E

N

A

N

T

T

A

P

I

R

H

Q

V

I

L

N

L

I

Q

L

R

R

L

A

L

L

G

G

L

G

P

E

V

V

P

P

D

V

F

Y

H

A

H

H

H

L

R

P

L
x
T

I

V

R

L

D

N

A

E

A

Q

G

G

K

E

R

K

L

M

A

S

K
|
K

R

R

D

H

D

G

S

A

K

M

A

S

L

V

A

M

K

G

Y

Y

R

R

A

D

E

A

G

G

M

Y

S

L

P

P

A

E

E

A

V

V

active site: S10 (= S11), T214 (≠ L240), K225 (= K251)
binding glutamic acid: R6 (= R7), A8 (= A9), S10 (= S11), E42 (= E41), R184 (= R210), H188 (≠ L214)

8vc5A Crystal structure of glutamyl-tRNA synthetase glurs from pseudomonas aeruginosa (zinc bound)
31% identity, 98% coverage: 6:276/277 of query aligns to 6:279/488 of 8vc5A

query
sites
8vc5A

T

T

R

R

F

I

A

A

P

P

S

S

P

P

T

T

G

G

P

D

L

P

H

H

L

V

G

G

H

T

A

A

F

Y

S

I

A

A

L

L

-

F

-

N

T

L

V

C

W

F

Q

A

R

R

A

Q

A

H

G

G

T

Q

A

F

L

I

L

L

R

R

I

I

E

E

D

D

I

T

D

D

R

Q

S

L

R

R

C

S

R

T

P

R

E

E

W

S

E

E

A

Q

Q

Q

I

I

F

Y

N

D

D

A

L

L

H

R

W

W

L

L

G

G

L

I

A

E

W

W

-

D

-

E

-

G

-

P

-

D

-

V

-

G

P

P

E

H

P

G

V

P

L

Y

R

R

Q

Q

S

S

D

E

R

R

G

G

G

H

A

I

Y

Y

R

K

A

K

A

Y

L

S

D

D

R

E

L

L

T

V

A

E

M

K

G

G

L

H

T

A

Y

F

P

T

C
|
C

R

F

C
|
C

S

T

-

P

-

E

R

R

A

L

D

D

-

A

I

V

R

R

A

A

A

E

L

Q

A

M

A

A

P

R

Q

K

E

E

G

T

V

P

P

R

V

-

G

-

P

-

D

-

G

-

I

-

V

-

Y
|
Y

P

D

G

G

T

H

C
|
C

R

M

G

H

R

L

P

-

M

-

A

-

E

-

A

-

G

-

P

P

D

K

D

D

A

E

I

V

R

Q

L

R

D

R

M

L

A

A

R

A

A

G

E

E

E

S

H

H

A

V

G

T

G

R

L

M

R

K

F

V

H

-

E

-

T

-

G

-

P

-

E

P

A

T

E

E

G

G

W

V

H

C

A

V

P

V

Q

P

D

D

L

M

A

L

R

R

S

-

V

-

G

G

D

D

V

V

-

E

-

I

-

P

-

W

-

D

-

R

-

M

-

D

-

M

-

Q

V

V

L

L

A

M

R

K

R

A

G

D

F

G

G

L

T

P

S

T

Y

Y

H

F

L

L

A

A

V

N

V

V

D

D

A

H

A

L

Q

M

G

G

V

I

T

T

E

H

V

V

V

L

R

R

G

G

A

E

D

E

L

W

F

L

E

P

A

S

T

A

A

P

I

K

H

L

V

I

L

K

L

L

Q

Y

R

E

L

Y

L

F

G

G

L

W

P

E

V

Q

P

P

D

Q

F

L

H

C

H

Y

H

M

R

P

L

L

I

L

R

R

D

N

A

P

A

D

G

K

K

S

R

K

L

L

A

S

K

K

R

R

D

K

D

N

S

P

K

T

A

S

L

I

A

T

K

F

Y

Y

R

E

A

R

E

M

G

G

M

Y

S

L

P

P

A

Q

E

A

V

L

R

L

A

N

L

Y

V

L

G

G

binding zinc ion: C107 (= C97), C109 (= C99), Y130 (= Y124), C134 (= C128)

4griB Crystal structure of a glutamyl-tRNA synthetase glurs from borrelia burgdorferi bound to glutamic acid and zinc (see paper)
31% identity, 97% coverage: 6:275/277 of query aligns to 4:277/485 of 4griB

query
sites
4griB

T

V

R
|
R

F

Y

A
|
A

P

P

S
|
S

P

P

T

T

G

G

P

L

L

Q

H

H

L

I

G

G

H

G

A

I

F

R

S

T

A

A

L

L

-

F

-

N

T

Y

V

F

W

F

Q

A

R

K

A

S

A

C

G

G

T

K

A

F

L

L

R

R

I

I

E
|
E

D

D

I

T

D

D

R

Q

S

S

R

R

C

Y

R

S

P

P

E

E

W

A

E

E

A

N

Q

D

I

L

F

Y

N

S

D

S

L

L

H

K

W

W

L

L

G

G

L

I

A

S

W

F

P

D

E

E

-

G

P

P

V

V

L

V

-

G

-

D

-

Y

-

A

-

P

-

Y

R

V

Q

Q

S

S

D

Q

R

R

G

S

G

A

A

I

Y

Y

R

K

A

Q

A

Y

L

A

D

K

R

Y

L

L

T

I

A

E

M

S

G

G

L

H

T

A

Y

Y

P

Y

C
|
C

R

Y

C
|
C

S

S

R

P

A

E

D

-

I

-

R

R

A

L

A

E

L

R

A

I

A

K

P

K

Q

I

E

Q

G

N

V

I

P

N

V

K

G

M

P

P

D

P

G

G

I

-

V

-

Y
|
Y

P

D

G

R

T

H

C
|
C

R

R

G

N

-

L

-

S

R

N

P

E

M

E

A

V

E

E

A

N

G

A

P

L

D

I

D

K

A

K

I

I

R

K

L

P

D

V

M

V

A

R

R

F

A

K

E

I

E

P

H

L

A

E

G

G

G

D

L

T

R

S

F

F

H

D

E

D

T

I

G

L

P

L

E

G

A

R

E

I

G

T

W

W

H

-

A

A

P

N

Q

K

D

D

L

I

A

-

R

-

S

S

V

P

G

D

D

P

V

V

V

I

L

L

A

K

R

S

R

D

G

G

F

L

G

P

T

T

S

-

Y
|
Y

H

H

L

L

A

A

V

N

V

V

D

D

A

Y

A

L

Q

M

G

K

V

I

T

T

E

H

V

V

V

L

R
|
R

G

A

A

Q

D

E

L
x
W

F

V

E

S

A

S

T

G

A

P

I

L

H

H

V

V

L

L

Q

Y

R

K

L

A

L

F

G

K

L

W

P

K

V

P

P

P

D

I

F

Y

H

C

H

H

H

L

R

P

L

M

I

V

R

M

D

G

A

N

A

D

G

G

K

Q

R

K

L

L

A

S

K
|
K

R

R

D

H

D

G

S

S

K

T

A

A

L

L

A

R

K

Q

Y

F

R

I

A

E

E

D

G

G

M

Y

S

L

P

P

A

E

E

A

V

I

R

I

A

N

L

Y

V

V

active site: S9 (= S11), K253 (= K251)
binding glutamic acid: R5 (= R7), A7 (= A9), S9 (= S11), E41 (= E41), Y194 (= Y192), R212 (= R210), W216 (≠ L214)
binding zinc ion: C105 (= C97), C107 (= C99), Y128 (= Y124), C132 (= C128)

3al0C Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
30% identity, 98% coverage: 6:277/277 of query aligns to 105:358/564 of 3al0C

query
sites
3al0C

T

V

R
|
R

F

F

A
|
A

P
|
P

S
|
S

P

P

T

T

G

G

P

H

L

L

H

H

L

V

G
|
G

H

G

A

A

F

R

S
x
T

A

A

L

L

T

F

V

N

W

W

Q

M

-

F

-

A

R

R

A

K

A

E

G

G

T

K

A

F

L

I

L

L

R

R

I

I

E
|
E

D

D

I
x
T

D
|
D

R

T

S

E

R
|
R

C

S

R

S

P

R

E

E

W

Y

E

E

A

Q

Q

Q

I

I

F

L

N

E

D

S

L

L

H

R

W

W

L

C

G

G

L

L

A

D

W

W

P

D

E

E

-

G

P

P

V

D

L

I

-

G

-

D

-

F

-

G

-

P

-

Y

R

R

Q

Q

S

S

D

E

R

R

G

L

G

E

A

I

Y

Y

R

R

A

E

A

Y

L

A

D

E

R

K

L

L

T

V

A

E

M

D

G

K

L

R

T

A

Y

Y

P

Y

C

V

R

V

C
x
Y

S

D

R

K

A

E

D

D

I
x
P

R

S

A

K

A

E

L

L

A

F

A

T

P

T

Q

Y

E

E

-

Y

-

P

-

H

-

E

-

Y

-

K

-

E

-

K

G

G

V

H

P

P

V

V

G

T

P

I

D

K

G

F

I

K

V

V

Y

L

P

P

G

G

T

-

C

-

R

-

G

-

R

-

P

-

M

-

A

-

E

K

A

T

G

S

P

F

D

E

D

D

A

L

I

L

R
x
K

L

-

D

-

M

-

A

-

R

-

A

-

E

-

H

-

A

-

G

G

G

Y

L
x
M

R

E

F

F

H

D

E

N

T

S

G

T

P

L

E

E

A

-

E

-

G

-

W

-

H

-

A

-

P

-

Q

-

D

-

L

-

A

-

R

-

S

-

V

-

G

D

D

F

V
x
I

V

I

L

M

A
x
K

R
x
S

R

N

G

G

F

F

G

P

T

T

S

-

Y
|
Y

H

N

L

F

A

A

V

V

D

D

A

H

A

L

Q

M

G

R

V

I

T

S

E

H

V

V

V

F

R
|
R

G
|
G

A

E

D
|
D

L
x
H

F
x
L

E
x
S

A
x
N

T

T

A

P

I
x
K

H

Q

V

L

L

M

L

I

Q

Y

R

E

L

A

L

F

G

G

L

W

P

E

V

A

P

P

D

V

F

F

H

M

H

H

H

I

R

P

L
|
L

I
|
I

R

L

D

G

A

S

A

D

G
x
R

K

T

R
x
P

L
|
L

A

S

K
|
K

R

R

D

H

D

G

S

A

K

T

A

S

L

V

A

E

K

H

Y

F

R

R

A

R

E

E

G

G

M

I

S

L

P

S

A

-

E

-

V

-

R

R

A

A

L

L

V

M

G

N

F

Y

active site: S110 (= S11), K335 (= K251)
binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R106 (= R7), A108 (= A9), P109 (= P10), G118 (= G19), T122 (≠ S23), E142 (= E41), Y276 (= Y192), R294 (= R210), G295 (= G211), D297 (= D213), H298 (≠ L214), L324 (= L240), I325 (= I241), L333 (= L249)
binding : T144 (≠ I43), D145 (= D44), R148 (= R47), Y208 (≠ C99), P213 (≠ I104), K252 (≠ R143), M255 (≠ L156), I266 (≠ V181), K269 (≠ A184), S270 (≠ R185), Y276 (= Y192), D297 (= D213), H298 (≠ L214), L299 (≠ F215), S300 (≠ E216), N301 (≠ A217), K304 (≠ I220), R330 (≠ G246), P332 (≠ R248)

Sites not aligning to the query:

binding : 363, 364, 365, 370, 387, 389, 391, 392, 397, 400, 407, 446, 447, 453, 457, 509, 520, 524, 527, 535, 536, 538, 539

6brlA Crystal structure of a glutamate tRNA ligase from elizabethkingia meningosepticum ccug26117 in complex with its amino acid (see paper)
30% identity, 91% coverage: 6:256/277 of query aligns to 5:266/502 of 6brlA

query
sites
6brlA

T

V

R
|
R

F

F

A
|
A

P

P

S
|
S

P

P

T

T

G

G

P

P

L

L

H

H

L

L

G

G

H

G

A

V

F

R

S

T

A

A

L

L

-

Y

-

D

T

Y

V

L

W

F

Q

A

R

K

A

H

A

N

G

G

T

D

A

F

L

I

L

L

R

R

I

I

E
|
E

D

D

I

T

D

D

R

T

S

Q

R

R

C

Y

R

V

P

P

E

G

W

S

E

E

A

E

Q

Y

I

I

F

M

N

E

D

A

L

L

H

E

W

W

L

I

G

G

L

M

A

V

-

P

-

D

-

E

-

S

-

P

-

K

-

H

-

G

W

G

P

P

E

Y

P

A

V

P

L

Y

R

R

Q

Q

S

S

D

E

R

R

G

R

G

D

A

I

Y

Y

R

-

A

-

L

-

D

D

R

R

L

Y

T

T

A

E

M

Q

G

I

L

L

T

K

Y

T

P

D

C

Y

R

A

C

Y

S

L

R

A

A

F

D

D

I

T

R

P

A

E

L

L

A

D

A

Q

P

I

Q

R

E

A

G

E

V

F

P

E

V

A

G

R

P

G

D

D

G

V

I

F

V

A

Y

Y

P

N

G

Y

T

E

C

T

R

R

G

N

R

R

P

-

M

-

A

-

E

-

A

-

G

-

P

-

D

-

A

-

I

L

R

R

L

N

D

S

M

I

A

S

R

L

A

P

E

E

H

E

A

V

G

K

L

L

R

L

F

E

H

E

E

K

T

T

G

-

P

P

E

Y

A

V

E

I

G

R

W

F

H

K

A

M

P

P

Q

L

D

D

L

R

A

I

R

I

S

N

V

L

G

N

D

D

V

I

L

R

A

G

R

K

R

F

G

S

F

V

G

N

T

T

-

N

-

T

-

L

-

D

-

K

-

V

-

L

-

V

-

K

-

N

-

D

-

G

-

M

-

P

S

T

Y
|
Y

H

H

L

F

A

A

V
x
N

V

I

D

D

A

H

A

E

Q

M

G

K

V

I

T

T

E

H

V

V

V

I

R
|
R

G

G

A

E

D

E

L
x
W

F

L

E

P

A

S

T

M

A

A

I

L

H

H

V

V

L

L

Q

Y

R

E

L

A

L

M

G

G

L

W

P

D

V

A

P

P

D

E

F

F

H

A

H

H

H

L

R

S

L

L

I

I

R

L

D

K

A

P

A

E

G

G

K

K

-

G

R

K

L

L

A

S

K
|
K

R

R

D

D

D

G

S

D

K

K

active site: K261 (= K251)
binding glutamic acid: R6 (= R7), A8 (= A9), S10 (= S11), E42 (= E41), Y201 (= Y192), N205 (≠ V196), R219 (= R210), W223 (≠ L214)

3aiiA Archaeal non-discriminating glutamyl-tRNA synthetase from methanothermobacter thermautotrophicus (see paper)
44% identity, 36% coverage: 5:105/277 of query aligns to 12:113/455 of 3aiiA

query
sites
3aiiA

V

V

T

L

R

R

F

F

A

A

P

P

S
x
N

P

P

T

S

G

G

P

P

L

L

H

H

L

I

G

G

H

H

A

A

F

R

S

A

A

A

L

I

T

L

V

N

-

H

-

E

W

Y

Q

A

R

R

A

K

A

Y

G

D

G

G

T

R

A

L

L

I

L

L

R

R

I

I

E

E

D

D

I

T

D

D

R

P

S

R

R

R

C

V

R

D

P

P

E

E

W

A

E

Y

A

D

Q

M

I

I

F

P

N

A

D

D

L

L

H

E

W

W

L

L

G

G

L

V

A

E

W

W

P

D

E

E

P

T

V

V

L

I

R

-

Q

Q

S

S

D

D

R

R

G

M

G

E

A

T

Y

Y

R

Y

A

E

A

Y

L

T

D

E

R

K

L

L

T

I

A

E

M

R

G

G

L

G

T

A

Y

Y

P

V

C
|
C

R

T

C
|
C

S

R

R

P

A

E

D

E

I

F

R

R

active site: N18 (≠ S11)
binding zinc ion: C105 (= C97), C107 (= C99)

Sites not aligning to the query:

active site: 231, 234
binding zinc ion: 122, 124

4h3sA The structure of glutaminyl-tRNA synthetase from saccharomyces cerevisiae (see paper)
32% identity, 37% coverage: 3:105/277 of query aligns to 36:140/585 of 4h3sA

query
sites
4h3sA

G

G

R

K

V

V

-

R

T

T

R

R

F

F

A

P

P

P

S
x
E

P

P

T

N

G

G

P

Y

L

L

H

H

L

I

G

G

H

H

A

S

F

-

S

K

A

A

L

I

T

M

V

V

-

N

-

F

-

G

W

Y

Q

A

R

K

A

Y

A

H

G

N

G

G

T

T

A

C

L

Y

L

L

R

R

I

F

E

D

D

D

I

T

D

N

R

P

S

E

R

K

C

E

R

A

P

P

E

E

W

Y

E

F

A

E

Q

S

I

I

F

K

N

R

D

M

L

V

H

S

W

W

L

L

G

G

L

F

A

K

W

-

P

P

E

W

P

K

V

I

L

T

R

Y

Q

S

S

S

D

D

R

Y

G

F

G

D

A

E

Y

L

R

Y

A

R

A

L

L

A

D

E

R

V

L

L

T

I

A

K

M

N

G

G

L

K

T

A

Y

Y

P

V

C
|
C

R

H

C
|
C

S

T

R

A

A

E

D

E

I

I

R

K

active site: E45 (≠ S11)
binding zinc ion: C132 (= C97), C134 (= C99)

Sites not aligning to the query:

active site: 271, 281
binding zinc ion: 155, 157

Query Sequence

>WP_011840919.1 NCBI__GCF_000015985.1:WP_011840919.1
MAGRVTRFAPSPTGPLHLGHAFSALTVWQRAAGGTALLRIEDIDRSRCRPEWEAQIFNDL
HWLGLAWPEPVLRQSDRGGAYRAALDRLTAMGLTYPCRCSRADIRAALAAPQEGVPVGPD
GIVYPGTCRGRPMAEAGPDDAIRLDMARAEEHAGGLRFHETGPEAEGWHAPQDLARSVGD
VVLARRGFGTSYHLAVVVDDAAQGVTEVVRGADLFEATAIHVLLQRLLGLPVPDFHHHRL
IRDAAGKRLAKRDDSKALAKYRAEGMSPAEVRALVGF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory