SitesBLAST
Comparing WP_011841005.1 NCBI__GCF_000015985.1:WP_011841005.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P60844 Aquaporin Z; Bacterial nodulin-like intrinsic protein; Water channel AqpZ from Escherichia coli (strain K12) (see paper)
49% identity, 85% coverage: 1:207/243 of query aligns to 1:208/231 of P60844
- C9 (≠ L9) mutation to S: No effect.
- C20 (= C20) mutation to S: Loss of oligomerization; no alteration of water permeability.
- T183 (≠ V182) mutation to C: No effect.
- R189 (= R188) mutation R->V,S: Loss of function.
2o9eA Crystal structure of aqpz mutant t183c complexed with mercury (see paper)
48% identity, 85% coverage: 1:207/243 of query aligns to 3:210/232 of 2o9eA
3nkaA Crystal structure of aqpz h174g,t183f (see paper)
48% identity, 85% coverage: 1:207/243 of query aligns to 3:210/230 of 3nkaA
P08995 Nodulin-26; N-26 from Glycine max (Soybean) (Glycine hispida) (see paper)
36% identity, 88% coverage: 3:217/243 of query aligns to 38:239/271 of P08995
Sites not aligning to the query:
- 262 modified: Phosphoserine; by CPK
P55088 Aquaporin-4; AQP-4; Mercurial-insensitive water channel; MIWC; WCH4 from Mus musculus (Mouse) (see 2 papers)
35% identity, 89% coverage: 3:219/243 of query aligns to 36:245/323 of P55088
- S111 (≠ P73) modified: Phosphoserine; by PKG; mutation to A: Loss of phosphorylation by PKG (in vitro). No effect on location at cell membrane.
Sites not aligning to the query:
- 4 natural variant: G -> R
P56402 Aquaporin-2; AQP-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct from Mus musculus (Mouse) (see 2 papers)
37% identity, 88% coverage: 2:214/243 of query aligns to 10:211/271 of P56402
- T126 (≠ S126) mutation to M: Does not cause loss of water channel activity, but impairs trafficking from cytoplasmic vesicles to the cell membrane.
Sites not aligning to the query:
- 256 modified: Phosphoserine; S → L: in cph; loss of a phosphorylation site and loss of trafficking to the apical cell membrane; causes aberrant location at the basolateral cell membrane
P41181 Aquaporin-2; AQP-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct from Homo sapiens (Human) (see 12 papers)
36% identity, 94% coverage: 2:229/243 of query aligns to 10:226/271 of P41181
- G64 (= G55) to R: in NDI2; loss of water channel activity; dbSNP:rs104894326
- G78 (≠ A69) mutation to A: Does not affect interaction with MIAC; when associated with A-79.
- C79 (≠ G70) mutation to A: Does not affect interaction with MIAC; when associated with A-78.
- S148 (≠ A148) mutation to A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; mutation to D: Retained in the endoplasmic reticulum.
- R187 (= R188) to C: in NDI2; loss of water channel activity; mutant protein does not fold properly; dbSNP:rs104894328
- A190 (= A191) to T: in NDI2; mutant protein does not fold properly and is not functional; dbSNP:rs104894341
- V194 (≠ F195) to I: in dbSNP:rs772051028
- S216 (≠ G219) to P: in NDI2; loss of water channel activity; dbSNP:rs104894329
- L217 (= L220) mutation to A: Abolishes interaction with MIAC; when associated with A-221.
- Y221 (≠ S224) mutation to A: Abolishes interaction with MIAC; when associated with A-217.
Sites not aligning to the query:
- 229 S→A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; S→D: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.
- 231 S→A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; S→D: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.
- 232 E→A: Reduces interaction with MIAC.
- 244 T→A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; T→E: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.
- 254 R → L: in NDI2; results in the loss of arginine vasopressin-mediated phosphorylation at S-256; R → Q: in NDI2; exerts a dominant-negative effect on wild-type-AQP2 in that it interferes with its trafficking to the apical membrane; is a loss of function instead of a gain of function mutation on dominant nephrogenic diabetes insipidus
- 256 modified: Phosphoserine; by PKA; S→A: Retained in vesicles.; S→D: Expressed in the apical membrane.
- 258 E → K: in NDI2; retained in the Golgi compartment; dbSNP:rs104894332
- 262 P → L: in NDI2; mutant protein folds properly and is functional but is retained in intracellular vesicles; able to assemble into tetramers with wild-type AQP2 that properly localize to the apical membrane; dbSNP:rs104894339; P→A: No effect on expression at the apical cell membrane.
4nefA X-ray structure of human aquaporin 2 (see paper)
36% identity, 94% coverage: 2:229/243 of query aligns to 9:225/239 of 4nefA
2evuA Crystal structure of aquaporin aqpm at 2.3a resolution (see paper)
34% identity, 91% coverage: 1:222/243 of query aligns to 4:240/245 of 2evuA
P09011 Lens fiber major intrinsic protein; Aquaporin-0; MIP26; MP26 from Rattus norvegicus (Rat) (see 2 papers)
35% identity, 88% coverage: 3:215/243 of query aligns to 9:210/261 of P09011
Sites not aligning to the query:
- 233 modified: Phosphoserine
- 244 N→D: No effects.
8sjxA Structure of lens aquaporin-0 array in sphingomyelin/cholesterol bilayer (2sm:1chol)
35% identity, 87% coverage: 3:214/243 of query aligns to 6:206/220 of 8sjxA
- binding cholesterol: I82 (≠ G78), F193 (≠ L201), W200 (≠ I208)
- binding [(E,2S,3R)-2-(hexadecanoylamino)-3-oxidanyl-octadec-4-enyl] 2-(trimethylazaniumyl)ethyl phosphate: F9 (≠ L6), F9 (≠ L6), F12 (≠ L9), V86 (≠ L82), V86 (≠ L82), L89 (≠ I85), L90 (≠ A86), L90 (≠ A86), L90 (≠ A86), Y100 (≠ F96), Y100 (≠ F96), S101 (≠ L97), I188 (≠ L194), L189 (≠ F195), R191 (≠ K199), F193 (≠ L201)
Sites not aligning to the query:
Q08733 Aquaporin PIP1-3; AtPIP1;3; Plasma membrane intrinsic protein 1c; PIP1c; Transmembrane protein B; TMP-B from Arabidopsis thaliana (Mouse-ear cress) (see paper)
35% identity, 89% coverage: 6:222/243 of query aligns to 55:273/286 of Q08733
Sites not aligning to the query:
- 1 modified: N-acetylmethionine
P55064 Aquaporin-5; AQP-5 from Homo sapiens (Human) (see 2 papers)
38% identity, 91% coverage: 3:222/243 of query aligns to 12:221/265 of P55064
- A38 (≠ I30) to E: in PPKB; retains the ability to traffic to the cell membrane; dbSNP:rs398123054
- I45 (= I35) to S: in PPKB; retains the ability to traffic to the cell membrane; dbSNP:rs398123055
- N123 (≠ T109) to D: in PPKB; retains the ability to traffic to the cell membrane; dbSNP:rs398123057
- S156 (= S155) mutation to A: No effect on location at the cell membrane.; mutation to E: Increased location at the cell membrane.
- I177 (= I177) to F: in PPKB; retains the ability to traffic to the cell membrane; dbSNP:rs398123056
- R188 (= R188) to C: in PPKB; retains the ability to traffic to the cell membrane; dbSNP:rs368292687
8ct2D Local refinement of aqp1 tetramer (c1; refinement mask included d1 of protein 4.2 and ankyrin-1 ar1-5) in class 2 of erythrocyte ankyrin-1 complex (see paper)
36% identity, 91% coverage: 3:222/243 of query aligns to 10:225/247 of 8ct2D
Q06611 Aquaporin PIP1-2; AtPIP1;2; Plasma membrane intrinsic protein 1b; PIP1b; Transmembrane protein A; AthH2; TMP-A from Arabidopsis thaliana (Mouse-ear cress) (see paper)
34% identity, 89% coverage: 6:222/243 of query aligns to 55:273/286 of Q06611
Sites not aligning to the query:
- 1 modified: N-acetylmethionine
Q39196 Probable aquaporin PIP1-4; Plasma membrane intrinsic protein 1-4; AtPIP1;4; Transmembrane protein C; TMP-C from Arabidopsis thaliana (Mouse-ear cress) (see paper)
34% identity, 89% coverage: 6:222/243 of query aligns to 56:274/287 of Q39196
Sites not aligning to the query:
- 1 modified: N-acetylmethionine
5dyeD Crystal structure of the full length s156e mutant of human aquaporin 5 (see paper)
36% identity, 91% coverage: 3:222/243 of query aligns to 11:220/253 of 5dyeD
P47863 Aquaporin-4; AQP-4; Mercurial-insensitive water channel; MIWC; WCH4 from Rattus norvegicus (Rat) (see 4 papers)
34% identity, 89% coverage: 3:219/243 of query aligns to 36:245/323 of P47863
- S180 (= S151) modified: Phosphoserine; by PKC; mutation to A: Decreases internalization from the cell membrane in response to PKC activation.
- H201 (= H173) mutation to P: Partial loss of transport activity.
Sites not aligning to the query:
- 13 modified: S-palmitoyl cysteine; C→A: Reduced palmitoylation. Loss of palmitoylation; when associated with A-17.
- 17 modified: S-palmitoyl cysteine; C→A: Reduced palmitoylation. Loss of palmitoylation; when associated with A-13.
- 285 modified: Phosphoserine
P06624 Lens fiber major intrinsic protein; Aquaporin-0; MIP26; MP26 from Bos taurus (Bovine) (see 4 papers)
34% identity, 87% coverage: 3:214/243 of query aligns to 11:211/263 of P06624
- Y149 (≠ A150) mutation to G: Increases constitutive water permeability. Abolishes regulation by cytoplasmic calcium levels.; mutation to L: Strongly decreases water permeability. Abolishes regulation by cytoplasmic calcium levels.; mutation to S: Slightly decreases water permeability, but has a minor effect on the regulation by cytoplasmic calcium levels.
Sites not aligning to the query:
- 227 L→A: Strongly reduced CALM binding.
- 227:237 Interaction with CALM
- 230 V→A: Strongly reduced CALM binding.
- 235 modified: Phosphoserine
- 243 modified: Phosphoserine; by PKA
- 245 modified: Phosphoserine
- 246 Interaction with BFSP1; modified: Deamidated asparagine
- 250 interaction with BFSP1
P30301 Lens fiber major intrinsic protein; Aquaporin-0; MIP26; MP26 from Homo sapiens (Human) (see 10 papers)
34% identity, 88% coverage: 3:215/243 of query aligns to 11:212/263 of P30301
- R33 (≠ L25) to C: in CTRCT15; reduces cell-to-cell adhesion, reduces cell-to-cell gap junction coupling, no loss of cell membrane localization, no loss of water channel activity; dbSNP:rs864309693
- V107 (≠ I98) to I: in CTRCT15; likely benign; dbSNP:rs74641138
- E134 (= E134) to G: in CTRCT15; non-progressive lamellar cataract; loss of activity; dbSNP:rs121917869
- T138 (= T138) to R: in CTRCT15; progressive polymorphic and lamellar cataract; loss of activity; dbSNP:rs121917867
- D150 (≠ S151) to H: in CTRCT15; loss of plasma membrane expression; dbSNP:rs778327521
- G165 (= G166) to D: in CTRCT15; uncertain significance; loss of plasma membrane expression
- Y177 (≠ N178) to C: in CTRCT15; uncertain significance
Sites not aligning to the query:
- 204:263 natural variant: Missing (in CTRCT15; uncertain significance)
- 211:263 natural variant: Missing (in CTRCT15; uncertain significance)
- 235 modified: Phosphoserine
- 246 modified: Deamidated asparagine; by deterioration
- 259 modified: Deamidated asparagine; by deterioration
Query Sequence
>WP_011841005.1 NCBI__GCF_000015985.1:WP_011841005.1
MTKKLLAELLGTFILVFFGCGAAVLMGPQIGMLGISLAFGLSIVAAAYSLGAISGAHLNP
AVSLGFLMAGRMPMAEFGGYVLAQIAGAFLGSLVVFLIASGKAGYVLATDGLGQNGFGAG
YLGEYSMGAALIFELIATFVFVSVILAATASHVSSASTALAGLAIGLTLTGIHLVGINVT
GVSVNPARSLAPALFVGGKALSDLWVFIVAPLAGGAAAGLAHASGFFRPGGIEPAPATGA
ATL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory