SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011841410.1 NCBI__GCF_000015985.1:WP_011841410.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q5F8J4 Homoserine dehydrogenase; HDH; HSD; NAD(+)-dependent homoserine dehydrogenase; NAD(+)-dependent HSD; NgHSD; EC 1.1.1.3 from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) (see paper)
39% identity, 95% coverage: 23:428/428 of query aligns to 22:433/435 of Q5F8J4

query
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L45 (≠ R46) mutation to R: Shows a marked increase in the catalytic efficiency with NADP(+).
LS 45:46 (≠ RD 46:47) mutation to RD: Does not impair the catalytic activity with NAD(+). Slightly increases the activity, but slightly decreases the affinity for NADP(+).; mutation to RR: Causes a shift in coenzyme preference from NAD(+) to NADP(+) by a factor of 974. Shows a slight decrease in the catalytic efficiency with NAD(+) and a 4.5-fold increase in catalytic efficiency with NADP(+).

6dzsA Mycobacterial homoserine dehydrogenase thra in complex with NADP
41% identity, 99% coverage: 4:426/428 of query aligns to 4:429/431 of 6dzsA

query
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binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G11), L12 (= L12), G13 (= G13), N14 (≠ T14), V15 (= V15), V45 (≠ A45), R46 (= R46), R47 (≠ D47), R52 (= R52), I63 (≠ V66), L78 (≠ V80), M79 (= M81), P84 (≠ A87), A102 (= A105), K104 (= K107), G306 (= G304), T310 (= T308)

4pg7A Crystal structure of s. Aureus homoserine dehydrogenase at ph7.5 (see paper)
35% identity, 74% coverage: 5:320/428 of query aligns to 5:307/402 of 4pg7A

query
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4pg7A

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active site: D190 (= D203), K194 (= K207)
binding lysine: R215 (≠ E228), G219 (≠ Q232)

Sites not aligning to the query:

binding lysine: 394, 395

6a0sA Homoserine dehydrogenase from thermus thermophilus hb8 complexed with hse and NADPH (see paper)
36% identity, 66% coverage: 5:286/428 of query aligns to 4:274/331 of 6a0sA

query
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6a0sA

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x
A

M
|
M

G

G

S

V

E

E

G

A

A

P

A

L

R

R

A

L

S

V

T

L

E

-

A

P

A

A

L

L

A

E

A

A

G

G

K

I

D

P

V

L

V

I

T

T

A
|
A

N

N

K
|
K

A

A

L

L

A

A

H

E

H

A

G

W

Q

E

A

S

L

L

A

R

E

P

M

F

A

A

E

E

A

E

A

-

G

G

L

L

A

-

I

I

R

Y

F

H

E

E

A

A

A

S

V

V

A

M

G

A

G

G

I

T

P

P

V

A

I

L

K

S

A

F

L

L

T

-

E

E

G

T

L

L

A

R

G

G

N

S

Q

E

M

L

R

L

R

E

V

L

M

H

G

G

V

I

M

L

N
|
N

G
|
G

T
|
T

C

T

N

L

Y

Y

I

I

L

L

T

Q

R

E

M

M

E

E

T

K

A

-

G

G

L

R

P

T

Y

Y

D

A

H

E

V

A

F

L

E

L

E

E

A

A

R

Q

Q

R

L

L

G
|
G

Y
|
Y

L

A

E
|
E

A

A

D

D

P

P

N

T

L

L

D
|
D

V

V

G

E

G

G

I

I

D
|
D

A

A

G

A

H

H

K
|
K

L

L

S

T

L

L

A

A

A

R

I

L

A

L

F

V

G

D

T

P

R

G

V

F

S

P

F

F

D

A

D

E

V

V

Q

E

L

A

E

Q

G

G

I

I

G

A

Q

R

I

L

S

T

I

P

D

E

D

V

I

L

R

Q

H

K

A

A

G

E

D

A

L

R

G

G

F

E

R

R

I

V

K

R

L

L

L

-

G

-

V

V

A

A

Q

S

V

L

S

F

G

G

R

E

G

G

L

G

E

R

Q

W

R

R

-

A

M

V

T

A

P

P

C

R

L

R

V

L

P

P

A

Q

D

D

S

H

P

P

L

L

G

A

Q

R

L

A

Q

R

G

G

G

N

T

A

N

L

M

W

V

V

active site: D191 (= D203), K195 (= K207)
binding l-homoserine: K99 (= K107), N150 (= N161), G151 (= G162), T152 (= T163), Y178 (= Y190), E180 (= E192), D186 (= D198), K195 (= K207)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (≠ L12), G12 (= G13), T13 (= T14), V14 (= V15), L42 (≠ C44), V43 (≠ A45), R44 (= R46), D45 (= D47), K48 (= K50), R50 (= R52), A73 (≠ V80), M74 (= M81), A97 (= A105), K99 (= K107), G177 (= G189), E180 (= E192)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 289, 290, 294

2ejwA Homoserine dehydrogenase from thermus thermophilus hb8
36% identity, 66% coverage: 5:286/428 of query aligns to 4:274/331 of 2ejwA

query
sites
2ejwA

L

L

R

K

L

I

G

A

I

L

A

L

G

G

L

G

G

G

T

T

V

V

G

G

I

S

G

A

V

F

V

Y

K

N

I

L

I

V

Q

L

R

E

H

R

A

A

D

E

L

E

I

L

A

S

A

A

R

F

A

G

G

V

R

V

P

P

V

R

I

F

I

L

T

-

A

G

V

V

C

L

A

V

R

R

D

D

R

P

S

R

K

K

N

P

R

R

D

A

A

I

D

-

L

-

S

-

G

-

Y

-

A

-

W

-

E

-

T

P

D

Q

A

E

V

L

A

L

L

R

A

A

Q

E

R

P

A

F

D

D

I

L

-

L

-

E

-

A

D

D

V

L

F

V

V

V

E

E

V

A

M

M

G

G

S

V

E

E

G

A

A

P

A

L

R

R

A

L

S

V

T

L

E

-

A

P

A

A

L

L

A

E

A

A

G

G

K

I

D

P

V

L

V

I

T

T

A

A

N

N

K

K

A

A

L

L

A

A

H

E

H

A

G

W

Q

E

A

S

L

L

A

R

E

P

M

F

A

A

E

E

A

E

A

-

G

G

L

L

A

-

I

I

R

Y

F

H

E
|
E

A

A

A

S

V
|
V

A
x
M

G
x
A

G

G

I
x
T

P

P

V

A

I

L

K

S

A

F

L

L

T

-

E

E

G

T

L

L

A

R

G

G

N

S

Q

E

M

L

R

L

R

E

V

L

M

H

G

G

V

I

M

L

N

N

G

G

T

T

C

T

N

L

Y

Y

I

I

L

L

T

Q

R

E

M

M

E

E

T

K

A

-

G

G

L

R

P

T

Y

Y

D

A

H

E

V

A

F

L

E

L

E

E

A

A

R

Q

Q

R

L

L

G

G

Y

Y

L

A

E

E

A

A

D

D

P

P

N

T

L

L

D

D

V

V

G

E

G

G

I

I

D
|
D

A

A

G

A

H

H

K
|
K

L

L

S

T

L

L

A

A

A

R

I

L

A

L

F

V

G

D

T

P

R

G

V

F

S

P

F

F

D

A

D

E

V

V

Q

E

L

A

E

Q

G

G

I

I

G

A

Q

R

I

L

S

T

I

P

D

E

D

V

I

L

R

Q

H

K

A

A

G

E

D

A

L

R

G

G

F

E

R

R

I

V

K

R

L

L

L

-

G

-

V

V

A

A

Q

S

V

L

S

F

G

G

R

E

G

G

L

G

E

R

Q

W

R

R

-

A

M

V

T

A

P

P

C

R

L

R

V

L

P

P

A

Q

D

D

S

H

P

P

L

L

G

A

Q

R

L

A

Q

R

G

G

G

N

T

A

N

L

M

W

V

V

active site: D191 (= D203), K195 (= K207)
binding magnesium ion: E121 (= E131), V124 (= V134), M125 (≠ A135), A126 (≠ G136), T128 (≠ I138)

6a0tB Homoserine dehydrogenase k99a mutant from thermus thermophilus hb8 complexed with hse and NADP+ (see paper)
36% identity, 66% coverage: 5:286/428 of query aligns to 4:274/332 of 6a0tB

query
sites
6a0tB

L

L

R

K

L

I

G

A

I

L

A

L

G
|
G

L
x
G

G
|
G

T
|
T

V
|
V

G

G

I

S

G

A

V

F

V

Y

K

N

I

L

I

V

Q

L

R

E

H

R

A

A

D

E

L

E

I

L

A

S

A

A

R

F

A

G

G

V

R

V

P

P

V

R

I

F

I

L

T

-

A

G

V

V

C
x
L

A
x
V

R
|
R

D
|
D

R

P

S

R

K
|
K

N

P

R
|
R

D

A

A

I

D

-

L

-

S

-

G

-

Y

-

A

-

W

-

E

-

T

P

D

Q

A

E

V

L

A

L

L

R

A

A

Q

E

R

P

A

F

D

D

I

L

-

L

-

E

-

A

D

D

V

L

F

V

V

V

E

E

V
x
A

M
|
M

G
|
G

G

G

S

V

E

E

G

A

A

P

A

L

R

R

A

L

S

V

T

L

E

-

A

P

A

A

L

L

A

E

A

A

G

G

K

I

D

P

V

L

V

I

T

T

A
|
A

N
|
N

K

K

A

A

L

L

A

A

H

E

H

A

G

W

Q

E

A

S

L

L

A

R

E

P

M

F

A

A

E

E

A

E

A

-

G

G

L

L

A

-

I

I

R

Y

F

H

E

E

A

A

A

S

V

V

A

M

G

A

G

G

I

T

P

P

V

A

I

L

K

S

A

F

L

L

T

-

E

E

G

T

L

L

A

R

G

G

N

S

Q

E

M

L

R

L

R

E

V

L

M

H

G

G

V

I

M

L

N
|
N

G
|
G

T
|
T

C

T

N

L

Y

Y

I

I

L

L

T

Q

R

E

M

M

E

E

T

K

A

-

G

G

L

R

P

T

Y

Y

D

A

H

E

V

A

F

L

E

L

E

E

A

A

R

Q

Q

R

L

L

G
|
G

Y
|
Y

L

A

E
|
E

A

A

D

D

P

P

N

T

L

L

D
|
D

V

V

G

E

G

G

I

I

D
|
D

A

A

G

A

H

H

K
|
K

L

L

S

T

L

L

A

A

A

R

I

L

A

L

F

V

G

D

T

P

R

G

V

F

S

P

F

F

D

A

D

E

V

V

Q

E

L

A

E

Q

G

G

I

I

G

A

Q

R

I

L

S

T

I

P

D

E

D

V

I

L

R

Q

H

K

A

A

G

E

D

A

L

R

G

G

F

E

R

R

I

V

K

R

L

L

L

-

G

-

V

V

A

A

Q

S

V

L

S

F

G

G

R

E

G

G

L

G

E

R

Q

W

R

R

-

A

M

V

T

A

P

P

C

R

L

R

V

L

P

P

A

Q

D

D

S

H

P

P

L

L

G

A

Q

R

L

A

Q

R

G

G

G

N

T

A

N

L

M

W

V

V

active site: D191 (= D203), K195 (= K207)
binding l-homoserine: N150 (= N161), G151 (= G162), T152 (= T163), Y178 (= Y190), E180 (= E192), D186 (= D198), K195 (= K207)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G11), G11 (≠ L12), G12 (= G13), T13 (= T14), V14 (= V15), L42 (≠ C44), V43 (≠ A45), R44 (= R46), D45 (= D47), K48 (= K50), R50 (= R52), A73 (≠ V80), M74 (= M81), G75 (= G82), A97 (= A105), N98 (= N106), G177 (= G189), E180 (= E192)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 289, 290, 294

7f4cA The crystal structure of the immature holo-enzyme of homoserine dehydrogenase complexed with NADP and 1,4-butandiol from the hyperthermophilic archaeon sulfurisphaera tokodaii. (see paper)
30% identity, 74% coverage: 5:321/428 of query aligns to 1:297/300 of 7f4cA

query
sites
7f4cA

L

M

R

K

L

L

G

L

I

L

A
x
F

G
|
G

L
x
Y

G
|
G

T
x
N

V
|
V

G

G

I

K

G

A

V

F

V

R

K

K

I

L

-

H

-

E

Q

K

R

R

H

S

A

P

D

E

L

L

I

-

A

-

R

-

A

-

G

-

R

N

P

D

V

V

I

I

T

G

A

G

V

I

C

V

A
x
T

R
|
R

D
x
R

R

G

S

I

K

M

N

L

R

Q

D

D

A

K

D

E

L

D

S

F

G

T

Y

P

A

D

W

L

E

E

T

G

D

D

A

V

V

F

A

K

L

A

A

F

Q

E

R

K

A

I

D

K

I

P

D

D

V

I

F

I

V

V

E

D

V
|
V

M
x
S

G
x
S

G

A

S

N

E

Y

G

N

A

N

A

G

R

E

A

P

S

S

-

L

-

S

-

L

T

Y

E

K

A

E

A

A

L

I

A

K

A

D

G

G

K

V

D

N

V

I

T

T

A
x
T

N

N

K
|
K

A

A

L

P

L

L

A

A

H

L

H

A

G

F

Q

N

A

E

L

I

A

F

E

S

M

L

A

A

E

R

A

S

A

K

G

G

L

V

A

K

I

I

R

G

F

F

E

Q

A
x
G

A

T

V

V

A

M

G
x
S

G

G

I

T

P

P

V

S

I

I

K

N

A

-

L

L

T

Y

E

R

G

V

L

L

A

P

G

G

N

S

Q

R

M

V

R

I

R

K

V

I

M

R

G

G

V

I

M

L

N

N

G

G

T

T

C

T

N

N

Y

F

I

I

L

L

T

T

R

L

M

M

E

-

T

N

A

K

G

G

L

V

P

S

Y

F

D

E

H

E

V

A

F

L

E

K

E

E

A

A

R

Q

Q

R

L

R

G

G

Y

Y

L

A

E
|
E

A

E

D

D

P

P

N

T

L

L

D

D

V

I

G

N

G

G

I

F

D

D

A

A

G

A

H

A

K

K

L

I

S

T

L

I

L

L

A

A

A

N

I

F

A

M

F

I

G

G

T

N

R

S

V

V

S

T

F

I

D

K

D

D

V

V

Q

K

L

F

E

E

G

G

I

I

G

N

Q

R

I

-

S

-

I

-

D

-

I

-

R

-

H

-

A

-

G

-

D

D

L

L

G

-

F

-

R

K

I

I

K

K

L

L

L

I

G

A

V

Y

A

A

Q

D

V

E

S

K

G

-

R

-

G

-

L

-

E

E

Q

V

R

W

M

V

T

K

P

P

C

L

L

P

V

I

P

S

A

Q

D

D

S

D

P

P

L

L

G

Y

Q

N

L

V

Q

D

G

G

G

V

T

E

N

N

M

A

V

L

V

E

L

I

E

T

G

T

D

D

A

-

V

I

G

Q

Q

S

I

I

V

L

L

I

R

R

G

G

P

P

G

G

A

A

G
|
G

E

P

G

V

P

N

T
x
A

A

A

S

Y

A

G

V

A

M

L

G

S

D

D

V

L

I

I

D

L

L

L

A

K

R

R

binding nadp nicotinamide-adenine-dinucleotide phosphate: F6 (≠ A10), G7 (= G11), Y8 (≠ L12), G9 (= G13), N10 (≠ T14), V11 (= V15), T37 (≠ A45), R38 (= R46), R39 (≠ D47), V72 (= V80), S73 (≠ M81), S74 (≠ G82), T100 (≠ A105), K102 (= K107), G127 (≠ A132), S131 (≠ G136), E185 (= E192), G280 (= G304), A284 (≠ T308)

F9VNG5 Homoserine dehydrogenase; HDH; HSD; StHSD; EC 1.1.1.3 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 3 papers)
30% identity, 74% coverage: 5:321/428 of query aligns to 1:301/304 of F9VNG5

query
sites
F9VNG5

L

M

R

K

L

L

G

L

I

L

A

F

G

G

L
x
Y

G

G

T
x
N

V
|
V

G

G

I

K

G

A

V

F

V

R

K

K

I

L

-

H

-

E

Q

K

R

R

H

S

A

P

D

E

L

L

I

-

A

-

R

-

A

-

G

-

R

N

P

D

V

V

I

I

T

G

A

G

V

I

C

V

A

T

R
|
R

D
x
R

R

G

S

I

K

M

N

L

R

Q

D

D

A

K

D

E

L

D

S

F

G

T

Y

P

A

D

W

L

E

E

T

G

D

D

A

V

V

F

A

K

L

A

A

F

Q

E

R

K

A

I

D

K

I

P

D

D

V

I

F

I

V

V

E

D

V

V

M
x
S

G

S

G

A

S

N

E

Y

G

N

A

N

A

G

R

E

A

P

S

S

-

L

-

S

-

L

T

Y

E

K

A

E

A

A

L

I

A

K

A

D

G

G

K

V

D

N

V

I

T

T

A
x
T

N

N

K
|
K

A

A

L

P

L

L

A

A

H

L

H

A

G

F

Q

N

A

E

L

I

A

F

E

S

M

L

A

A

E

R

A

S

A

K

G

G

L

V

A

K

I

I

R

G

F

F

E

Q

A

G

A

T

V

V

A

M

G

S

G

G

I

T

P

P

V

S

I

I

K

N

A

-

L

L

T

Y

E

R

G

V

L

L

A

P

G

G

N

S

Q

R

M

V

R

I

R

K

V

I

M

R

G

G

V

I

M

L

N

N

G

G

T

T

C

T

N

N

Y

F

I

I

L

L

T

T

R

L

M

M

E

-

T

N

A

K

G

G

L

V

P

S

Y

F

D

E

H

E

V

A

F

L

E

K

E

E

A

A

R

Q

Q

R

L

R

G
|
G

Y

Y

L

A

E
|
E

A

E

D

D

P

P

N

T

L

L

D

D

V

I

G

N

G

G

I

F

D

D

A

A

G

A

H

A

K

K

L

I

S

T

L

I

L

L

A

A

A

N

I

F

A

M

F

I

G

G

T

N

R

S

V

V

S

T

F

I

D

K

D

D

V

V

Q

K

L

F

E

E

G

G

I

I

G

N

Q

R

I

-

S

-

I

-

D

-

I

-

R

-

H

-

A

-

G

-

D

D

L

L

-

P

-

K

G

N

F

E

R

K

I

I

K

K

L

L

L

I

G

A

V

Y

A

A

Q

D

V

E

S

K

G

-

R

-

G

-

L

-

E

E

Q

V

R

W

M

V

T

K

P

P

C

L

L

P

V

I

P

S

A

Q

D

D

S

D

P

P

L

L

G

Y

Q

N

L

V

Q

D

G

G

G

V

T

E

N

N

M

A

V

L

V

E

L

I

E

T

G

T

D

D

A

-

V

I

G

Q

Q

S

I

I

V

L

L

I

R

R

G

G

P

P

G

G

A

A

G
|
G

E

P

G

V

P

N

T

A

A

A

S

Y

A

G

V

A

M

L

G

S

D

D

V

L

I

I

D

L

L

L

A

K

R

R

Y8 (≠ L12) binding NADP(+)
N10 (≠ T14) binding NADP(+)
V11 (= V15) binding NADP(+)
R38 (= R46) binding NADP(+)
R39 (≠ D47) binding NADP(+)
S73 (≠ M81) binding NADP(+)
T100 (≠ A105) binding NADP(+)
K102 (= K107) binding NADP(+)
G182 (= G189) binding NADP(+)
E185 (= E192) binding NADP(+)
G284 (= G304) binding NADP(+)

Sites not aligning to the query:

303 modified: Interchain

4xb1A Hyperthermophilic archaeal homoserine dehydrogenase in complex with NADPH (see paper)
28% identity, 76% coverage: 5:328/428 of query aligns to 3:318/319 of 4xb1A

query
sites
4xb1A

L

V

R

N

L

I

G

S

I

I

A
x
F

G

G

L
x
F

G
|
G

T
|
T

V
|
V

G

G

I

R

G

A

V

L

V

A

K

E

I

I

Q

A

R

E

H

K

A

S

D

R

L

I

I

F

A

G

A

-

R

-

A

-

G

-

R

-

P

-

V

V

I

E

I

L

T

N

A

V

V

I

C

S

A

I

R

T

D
|
D

R
|
R

S

S

-

G

-

T

-

I

-

W

-

G

-

D

-

F

-

D

-

L

-

E

-

A

-

K

-

E

-

V

K
|
K

N

E

R

S

D

T

A

G

D

K

L

L

S

S

G

N

Y

I

-

G

-

D

-

Y

-

E

A

V

W

Y

E

N

T

F

D

S

A

P

V

Q

A

E

L

L

A

V

Q

E

R

E

A

V

D

K

I

P

D

N

V

I

F

L

V

V

E

D

V
|
V

M
x
S

G
x
S

G

W

S

D

E

E

G

-

A

-

A

A

R

H

A

E

S

M

T

Y

E

K

A

V

A

A

L

L

A

G

A

E

G

G

K

I

D

S

V

V

T

T

A
x
S

N

N

K
|
K

A

P

L

P

L

I

A

A

H

N

H

Y

G

Y

Q

D

A

E

L

L

A

M

E

N

M

L

A

A

E

K

A

E

A

N

G

N

L

A

A

G

I

I

R

F

F

F

E

E

A
x
S

A

T

V

V

A

M

G

A

G

G

I

T

P

P

V

I

I

I

K

G

A

V

L

L

T

R

E

E

G

N

L

L

A

L

G

G

N

E

Q

N

M

I

R

K

R

R

V

I

M

D

G

A

V

V

M

V

N

N

G

A

T

S

C

T

N

T

Y

F

I

I

L

L

T

T

R

K

M

M

E

-

T

S

A

E

G

G

L

K

P

T

Y

L

D

D

H

D

V

A

F

I

E

E

A

A

R

K

Q

S

L

L

G

G

Y

I

L

L

E

E

A

E

D

D

P

P

N

S

L

K

D

D

V

I

G

D

G

G

I

I

D
|
D

A

A

G

Y

H

Y

K
|
K

L

A

S

K

L

I

L

L

A

H

A

W

I

V

A

S

F

Y

G

G

T

E

R

P

V

P

S

E

F

E

D

E

V

-

Q

-

L

-

E

-

G

-

I

-

G

-

Q

R

I

L

S

G

I

I

D

R

D

E

I

V

R

R

H

D

A

A

G

R

D

N

L

V

G

R

F

L

R

-

I

-

K

-

L

-

G

-

V

V

A

A

Q

Q

V

V

S

S

G

K

R

G

G

K

L

I

E

-

Q

-

R

S

M

V

T

K

P

P

C

R

L

K

V

L

P

S

A

S

D

D

S

N

P

P

L

L

G

-

Q

L

L

V

Q

E

G

G

G

V

T

Q

N

N

M

A

V

A

V

V

L

I

E

R

G

T

D

N

A

N

V

L

G

G

Q

E

I

V

V

I

L

L

R

K

G

G

P

P

G

G

A
x
G

G

G

E

G

G

R

P

V

T
|
T

A

A

S

S

A

G

V

V

M

F

G

T

D

D

V

I

I

I

D

K

L

A

A

T

R

-

G

-

L

L

R

K

L

F

P

P

T

N

F

L

active site: D211 (= D203), K215 (= K207)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ A10), F10 (≠ L12), G11 (= G13), T12 (= T14), V13 (= V15), D39 (= D47), R40 (= R48), K57 (= K50), V91 (= V80), S92 (≠ M81), S93 (≠ G82), S114 (≠ A105), K116 (= K107), S141 (≠ A132), G295 (≠ A303), T300 (= T308)

O58802 Homoserine dehydrogenase; HDH; HSD; HseDH; EC 1.1.1.3 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
28% identity, 76% coverage: 5:328/428 of query aligns to 3:318/319 of O58802

query
sites
O58802

L

V

R

N

L

I

G

S

I

I

A

F

G

G

L
x
F

G

G

T
|
T

V
|
V

G

G

I

R

G

A

V

L

V

A

K

E

I

I

Q

A

R

E

H

K

A

S

D

R

L

I

I

F

A

G

A

-

R

-

A

-

G

-

R

-

P

-

V

V

I

E

I

L

T

N

A

V

V

I

C

S

A

I

R

T

D

D

R
|
R

S

S

-

G

-

T

-

I

-

W

-

G

-

D

-

F

-

D

-

L

-

E

-

A

-

K

-

E

-

V

K
|
K

N

E

R

S

D

T

A

G

D

K

L

L

S

S

G

N

Y

I

-

G

-

D

-

Y

-

E

A

V

W

Y

E

N

T

F

D

S

A

P

V

Q

A

E

L

L

A

V

Q

E

R

E

A

V

D

K

I

P

D

N

V

I

F

L

V

V

E

D

V

V

M
x
S

G
x
S

G

W

S

D

E

E

G

-

A

-

A

A

R

H

A

E

S

M

T

Y

E

K

A

V

A

A

L

L

A

G

A

E

G

G

K

I

D

S

V

V

T

T

A
x
S

N

N

K
|
K

A

P

L

P

L

I

A

A

H

N

H

Y

G

Y

Q

D

A

E

L

L

A

M

E

N

M

L

A

A

E

K

A

E

A

N

G

N

L

A

A

G

I

I

R

F

F

F

E
|
E

A

S

A

T

V
|
V

A

M

G
x
A

G

G

I
x
T

P

P

V

I

I

I

K

G

A

V

L

L

T

R

E

E

G

N

L

L

A

L

G

G

N

E

Q

N

M

I

R

K

R

R

V

I

M

D

G

A

V

V

M

V

N

N

G

A

T

S

C

T

N

T

Y

F

I

I

L

L

T

T

R

K

M

M

E

-

T

S

A

E

G

G

L

K

P

T

Y

L

D

D

H

D

V

A

F

I

E

E

A

A

R

K

Q

S

L

L

G

G

Y

I

L

L

E

E

A

E

D

D

P

P

N

S

L

K

D

D

V

I

G

D

G

G

I

I

D

D

A

A

G

Y

H

Y

K

K

L

A

S

K

L

I

L

L

A

H

A

W

I

V

A

S

F

Y

G

G

T

E

R

P

V

P

S

E

F

E

D

E

V

-

Q

-

L

-

E

-

G

-

I

-

G

-

Q

R

I

L

S

G

I

I

D

R

D

E

I

V

R

R

H

D

A

A

G

R

D

N

L

V

G

R

F

L

R

-

I

-

K

-

L

-

G

-

V

V

A

A

Q

Q

V

V

S

S

G

K

R

G

G

K

L

I

E

-

Q

-

R

S

M

V

T

K

P

P

C

R

L

K

V

L

P

S

A

S

D

D

S

N

P

P

L

L

G

-

Q

L

L

V

Q

E

G

G

G

V

T

Q

N

N

M

A

V

A

V

V

L

I

E

R

G

T

D

N

A

N

V

L

G

G

Q

E

I

V

V

I

L

L

R

K

G

G

P

P

G

G

A

G

G
|
G

E

G

G

R

P

V

T

T

A

A

S

S

A

G

V

V

M

F

G

T

D

D

V

I

I

I

D

K

L

A

A

T

R

-

G

-

L

L

R

K

L

F

P

P

T

N

F

L

F10 (≠ L12) binding NADPH
T12 (= T14) binding NADPH
V13 (= V15) binding NADPH
R40 (= R48) binding NADPH; mutation to A: Increases activity with NADP.
K57 (= K50) binding NADPH; mutation to A: Increases activity with NADP.
S92 (≠ M81) binding NADPH
S93 (≠ G82) binding NADPH
S114 (≠ A105) binding NADPH
K116 (= K107) binding NADPH
E140 (= E131) binding Na(+)
V143 (= V134) binding Na(+)
A145 (≠ G136) binding Na(+)
T147 (≠ I138) binding Na(+)
G296 (= G304) binding NADPH

4xb2A Hyperthermophilic archaeal homoserine dehydrogenase mutant in complex with NADPH (see paper)
31% identity, 61% coverage: 68:328/428 of query aligns to 79:318/319 of 4xb2A

query
sites
4xb2A

L

L

A

V

Q

E

R

E

A

V

D

K

I

P

D

N

V

I

F

L

V

V

E

D

V
|
V

M
x
S

G
x
S

G

W

S

D

E

E

G

-

A

-

A

A

R

H

A

E

S

M

T

Y

E

K

A

V

A

A

L

L

A

G

A

E

G

G

K

I

D

S

V

V

T

T

A
x
S

N
|
N

K
|
K

A

P

L

P

L

I

A

A

H

N

H

Y

G

Y

Q

D

A

E

L

L

A

M

E

N

M

L

A

A

E

K

A

E

A

N

G

N

L

A

A

G

I

I

R

F

F

F

E

E

A
x
S

A

T

V

V

A

M

G

A

G

G

I

T

P

P

V

I

I

I

K

G

A

V

L

L

T

R

E

E

G

N

L

L

A

L

G

G

N

E

Q

N

M

I

R

K

R

R

V

I

M

D

G

A

V

V

M

V

N

N

G
x
A

T
x
S

C

T

N

T

Y

F

I

I

L

L

T

T

R

K

M

M

E

-

T

S

A

E

G

G

L

K

P

T

Y

L

D

D

H

D

V

A

F

I

E

E

A

A

R

K

Q

S

L

L

G

G

Y

I

L

L

E

E

A

E

D

D

P

P

N

S

L

K

D
|
D

V

I

G

D

G

G

I

I

D
|
D

A

A

G

Y

H

Y

K
|
K

L

A

S

K

L

I

L

L

A

H

A

W

I

V

A

S

F

Y

G

G

T

E

R

P

V

P

S

E

F

E

D

E

V

-

Q

-

L

-

E

-

G

-

I

-

G

-

Q

R

I

L

S

G

I

I

D

R

D

E

I

V

R

R

H

D

A

A

G

R

D

N

L

V

G

R

F

L

R

-

I

-

K

-

L

-

G

-

V

V

A

A

Q

Q

V

V

S

S

G

K

R

G

G

K

L

I

E

-

Q

-

R

S

M

V

T

K

P

P

C

R

L

K

V

L

P

S

A

S

D

D

S

N

P

P

L

L

G

-

Q

L

L

V

Q

E

G

G

G

V

T

Q

N

N

M

A

V

A

V

V

L

I

E

R

G

T

D

N

A

N

V

L

G

G

Q

E

I

V

V

I

L

L

R

K

G

G

P

P

G

G

A
x
G

G

G

E

G

G

R

P

V

T
|
T

A

A

S

S

A

G

V

V

M

F

G

T

D

D

V

I

I

I

D

K

L

A

A

T

R

-

G

-

L

L

R

K

L

F

P

P

T

N

F

L

active site: D211 (= D203), K215 (= K207)
binding l-homoserine: A171 (≠ G162), S172 (≠ T163), D206 (= D198), K215 (= K207)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V91 (= V80), S92 (≠ M81), S93 (≠ G82), S114 (≠ A105), N115 (= N106), K116 (= K107), S141 (≠ A132), G295 (≠ A303), T300 (= T308)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 8, 10, 11, 12, 13, 40

5x9dA Crystal structure of homoserine dehydrogenase in complex with l- cysteine and NAD (see paper)
30% identity, 74% coverage: 5:321/428 of query aligns to 1:299/302 of 5x9dA

query
sites
5x9dA

L

M

R

K

L

L

G

L

I

L

A
x
F

G
|
G

L
x
Y

G
|
G

T
x
N

V
|
V

G

G

I

K

G

A

V

F

V

R

K

K

I

L

-

H

-

E

Q

K

R

R

H

S

A

P

D

E

L

L

I

-

A

-

R

-

A

-

G

-

R

N

P

D

V

V

I

I

T

G

A

G

V

I

C

V

A
x
T

R
|
R

D

R

R

G

S

I

K

M

N

L

R

Q

D

D

A

K

D

E

L

D

S

F

G

T

Y

P

A

D

W

L

E

E

T

G

D

D

A

V

V

F

A

K

L

A

A

F

Q

E

R

K

A

I

D

K

I

P

D

D

V

I

F

I

V

V

E

D

V
|
V

M
x
S

G
x
S

G

A

S

N

E

Y

G

N

A

N

A

G

R

E

A
x
P

S

S

-

L

-

S

-

L

T

Y

E

K

A

E

A

A

L

I

A

K

A

D

G

G

K

V

D

N

V

I

T

T

A
x
T

N
|
N

K
|
K

A

A

L

P

L

L

A

A

H

L

H

A

G

F

Q

N

A

E

L

I

A

F

E

S

M

L

A

A

E

R

A

S

A

K

G

G

L

V

A

K

I

I

R

G

F

F

E

Q

A
x
G

A

T

V

V

A

M

G
x
S

G

G

I

T

P

P

V

S

I

I

K

N

A

-

L

L

T

Y

E

R

G

V

L

L

A

P

G

G

N

S

Q

R

M

V

R

I

R

K

V

I

M

R

G

G

V

I

M

L

N
|
N

G
|
G

T
|
T

C

T

N

N

Y

F

I

I

L

L

T

T

R

L

M

M

E

-

T

N

A

K

G

G

L

V

P

S

Y

F

D

E

H

E

V

A

F

L

E

K

E

E

A

A

R

Q

Q

R

L

R

G

G

Y
|
Y

L
x
A

E
|
E

A

E

D

D

P

P

N

T

L

L

D
|
D

V

I

G

N

G

G

I

F

D
|
D

A

A

G

A

H

A

K
|
K

L

I

S

T

L

I

L

L

A

A

A

N

I

F

A

M

F

I

G

G

T

N

R

S

V

V

S

T

F

I

D

K

D

D

V

V

Q

K

L

F

E

E

G

G

I

I

G

N

Q

R

I

-

S

-

I

-

D

-

D

D

I

P

R

K

H

N

A

E

G

-

D

-

L

-

G

-

F

-

R

K

I

I

K

K

L

L

L

I

G

A

V

Y

A

A

Q

D

V

E

S

K

G

-

R

-

G

-

L

-

E

E

Q

V

R

W

M

V

T

K

P

P

C

L

L

P

V

I

P

S

A

Q

D

D

S

-

P

P

L

L

G

Y

Q

N

L

V

Q

D

G

G

G

V

T

E

N

N

M

A

V

L

V

E

L

I

E

T

G

T

D

D

A

-

V

I

G

Q

Q

S

I

I

V

L

L

I

R

R

G

G

P

P

G

G

A
|
A

G
|
G

E

P

G

V

P

N

T
x
A

A

A

S

Y

A

G

V

A

M

L

G

S

D

D

V

L

I

I

D

L

L

L

A

K

R

R

active site: D196 (= D203), K200 (= K207)
binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: F6 (≠ A10), G7 (= G11), Y8 (≠ L12), G9 (= G13), N10 (≠ T14), V11 (= V15), T37 (≠ A45), R38 (= R46), V72 (= V80), S73 (≠ M81), S74 (≠ G82), P82 (≠ A90), T100 (≠ A105), N101 (= N106), K102 (= K107), G127 (≠ A132), S131 (≠ G136), N155 (= N161), G156 (= G162), T157 (= T163), Y183 (= Y190), A184 (≠ L191), E185 (= E192), D191 (= D198), D196 (= D203), K200 (= K207), A281 (= A303), G282 (= G304), A286 (≠ T308)

3ingA Crystal structure of homoserine dehydrogenase (np_394635.1) from thermoplasma acidophilum at 1.95 a resolution
27% identity, 54% coverage: 5:236/428 of query aligns to 3:242/319 of 3ingA

query
sites
3ingA

L

I

R

R

L

I

G

I

I

L

A

M

G
|
G

L
x
T

G
|
G

T
x
N

V
|
V

G

G

I

L

G

N

V

V

V

L

K

R

I

I

Q

D

R

A

H

S

-

N

-

R

-

F

-

S

-

I

-

K

-

V

-

G

-

V

A

S

D
|
D

L
x
S

I

R

A

S

A

Y

R

A

A

S

G

G

R

R

P

N

V

L

I

D

I

I

T

S

A

S

V

I

C

I

A

S

R

-

D

-

R

-

S

N

K
|
K

N

E

R

K

D

T

A

G

D

R

L

I

S

S

G

D

Y

R

A

A

W

F

E

S

T

G

D

P

A

E

V

D

A

L

L

M

A

G

Q

E

R

A

A

A

D

D

I

L

D

-

V

-

F

L

V

V

E

D

V
x
C

M
x
T

G
x
P

G

A

S

S

E

R

G

D

A

G

A

V

R

R

A

E

S

Y

T

S

-

L

-

Y

E

R

A

M

A

A

L

F

A

E

A

S

G

G

K

M

D

N

V

V

T

T

A
|
A

N
|
N

K
|
K

A

S

L

G

L

L

A

A

H

N

H

K

G

W

Q

H

A

D

L

I

A

M

E

D

M

S

A

A

E

N

A

Q

A

N

G

S

L

K

A

Y

I

I

R

R

F

Y

E

E

A
|
A

A

T

V

V

A

A

G

G

I

V

P

P

V

L

I

F

K

S

A

V

L

L

T

D

E

Y

G

S

L

I

A

L

G

P

N

S

Q

K

M

V

R

K

R

R

V

F

M

R

G

G

V

I

M

V

N

S

G

S

T

T

C

I

N

N

Y

Y

I

V

L

I

T

R

R

N

M

M

E

A

T

N

A

-

G

G

L

R

P

S

Y

L

D

R

H

D

V

V

F

V

E

D

A

A

R

I

Q

K

L

K

G

G

Y

I

L

A

E
|
E

A
x
S

D

N

P

P

N

Q

L

D

D

D

V

L

G

N

G

G

I

L

D
|
D

A

A

G

A

H

R

K
|
K

L

S

S

V

L

I

L

L

A

V

A

N

I

H

A

I

F

F

G

G

T

T

R

E

V

Y

S

T

F

L

D

N

D

D

V

V

Q

E

L

Y

E

S

G

G

I

V

G

D

Q

E

I

R

S

S

I

Y

D

N

active site: D209 (= D203), K213 (= K207)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G11), T10 (≠ L12), G11 (= G13), N12 (≠ T14), V13 (= V15), D38 (= D28), S39 (≠ L29), K57 (= K50), C85 (≠ V80), T86 (≠ M81), P87 (≠ G82), A112 (= A105), N113 (= N106), K114 (= K107), A139 (= A132), E198 (= E192), S199 (≠ A193)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 302, 305

3jsaA Homoserine dehydrogenase from thermoplasma volcanium complexed with NAD
26% identity, 74% coverage: 5:320/428 of query aligns to 4:320/321 of 3jsaA

query
sites
3jsaA

L

I

R

N

L

L

G

S

I

I

A

F

G
|
G

L
|
L

G
|
G

T
x
N

V
|
V

G

G

I

L

G

N

V

L

K

R

I

I

Q

R

R

S

H

F

-

N

-

E

-

N

-

R

-

L

-

G

-

L

-

K

-

F

-

N

-

V

-

F

-

V

A

A

D
|
D

L
x
S

I

L

A

H

A

S

R

Y

A

Y

G

N

R

E

P

R

V

I

I

D

I

I

T

G

A

K

V

V

C

I

A

-

R

-

D

-

R

-

S

S

K

Y

N

K

R

E

D

K

A

G

D

S

L

L

S

D

G

S

Y

L

A

E

W

Y

E

E

T

S

D

I

A

S

V

A

A

S

L

E

A

A

Q

L

R

A

A

R

D

D

I

F

D

D

V

I

F

V

V

V

E

D

V
x
A

M
x
T

G
x
P

G

A

S

S

E

A

G

D

A

G

A

K

R

K

-

E

-

L

A

A

S

F

T

Y

E

K

A

E

A

T

L

F

A

E

A

N

G

G

K

K

D

D

V

V

T

T

A
|
A

N
|
N

K

K

A

S

L

G

L

L

A

A

H

N

H

F

G

W

Q

P

A

E

L

I

A

M

E

E

M

Y

A

A

E

R

A

S

A

N

G

N

L

R

A

R

I

I

R

R

F

Y

E

E

A
|
A

A

T

V

V

A

A

G

G

I

V

P

P

V

L

I

F

K

S

A

F

L

I

T

D

E

Y

G

S

L

V

A

L

G

P

N

S

Q

R

M

I

R

K

V

F

M

R

G

G

V

I

M

V

N

S

G

L

T

T

C

I

N

N

Y

Y

I

F

L

I

T

R

R

E

M

L

E

A

T

N

A

K

G

R

L

-

P

E

Y

F

D

D

H

D

V

V

F

L

E

S

E

E

A

A

R

T

Q

K

L

L

G

G

Y

I

L

V

E

N

A

Y

D

K

P

D

N

-

L

-

D

D

V

L

G

T

G

G

I

L

D
|
D

A

A

G

A

H

R

K
|
K

L

S

S

V

L

I

L

L

A

C

A

N

I

H

A

L

F

Y

G

G

T

S

R

S

V

Y

S

R

F

L

D

S

D

D

V

V

Q

F

L

Y

E

E

G

G

I

I

G

-

Q

-

I

-

S

-

I

L

D

D

D

-

I

-

R

R

H

S

A

F

G

G

D

K

L

N

G

E

F

R

R

L

I

V

K

T

L

E

L

T

G

G

V

I

A

-

Q

-

V

V

S

N

G

G

R

K

-

P

G

S

L

A

E

E

Q

S

R

R

M

I

T

K

P

S

C

-

L

-

V

L

P

D

A

S

D

N

S

D

P

Y

L

L

G

L

Q

T

L

L

Q

G

G

K

G

G

T

S

N

L

M

G

V

Y

V

Q

L

L

E

Q

G

T

D

D

A

T

V

N

G

G

Q

T

I

L

V

N

L

V

R

S

G

D

P

L

G

Y

A

D

G

G

E

P

G

Y

P

E

T
|
T

A

A

S

G

A

A

V

V

M

M

G

N

D

D

V

L

I

V

D

I

L

L

A

S

active site: D212 (= D203), K216 (= K207)
binding nicotinamide-adenine-dinucleotide: G10 (= G11), L11 (= L12), G12 (= G13), N13 (≠ T14), V14 (= V15), D42 (= D28), S43 (≠ L29), A90 (≠ V80), T91 (≠ M81), P92 (≠ G82), A117 (= A105), N118 (= N106), A144 (= A132), T308 (= T308)

O81852 Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic; AK-HD 2; AK-HSDH 2; Beta-aspartyl phosphate homoserine 2; EC 2.7.2.4; EC 1.1.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
23% identity, 75% coverage: 5:327/428 of query aligns to 558:916/916 of O81852

query
sites
O81852

L

L

R

A

L

M

G

G

I

I

A

V

G

G

L

P

G

G

T

L

V

I

G

G

I

A

G

T

V

L

K

D

I

Q

I

L

Q

R

R

D

H

Q

A

A

D

A

L

V

I

L

A

K

A

Q

R

E

A

F

G

N

R

I

P

D

V

L

I

R

I

V

T

L

A

G

V

I

C

T

A

G

R

S

D

K

R

K

S

M

K

L

N

L

R

S

D

D

A

I

D

G

L

I

S

D

G

L

Y

S

A

R

W

W

E

R

T

E

D

L

A

L

V

N

A

E

L

K

A

G

Q

T

R

E

A

A

D

D

I

L

D

D

V

K

F

F

V

T

E

Q

V

Q

M

V

G

H

G

G

S

N

E

H

G

F

A

I

A

P

R

N

A

S

-

V

-

D

-

C

S

T

T

A

E

D

A

S

A

A

L

I

A

A

A

S

-

R

-

Y

-

D

-

W

-

L

-

R

-

K

G

G

K

I

D

H

V

V

V

I

T

T

A

P

N

N

K

K

A

K

L

-

A

A

H

N

H

S

G

G

Q

P

-

L

-

D

-

Q

-

Y

-

L

A

K

L

L

A

R

E

D

M

L

A

Q

E

R

A

K

A

S

G

Y

L

T

A

H

I

Y

R

F

F

Y

E

E

A

A

A

T

V

V

A

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

A

T

L

L

T

-

E

R

G

G

L

L

-

L

-

E

A

T

G

G

N

D

Q

K

M

I

R

L

R

R

V

I

M

E

G

G

V

I

M

C

N

S

G

G

T

T

C

L

N

S

Y

Y

I

L

L

F

T

N

R

N

M

F

E

-

T

V

A

G

G

D

L

R

P

S

Y

F

D

S

H

E

V

V

F

V

E

T

E

E

A

A

R

K

Q

N

L

A

G

G

Y

F

L

T

E

E

A

P

D

D

P

P

N

R

L

D

D

D

V

L

G

S

G

G

I

T

D

D

A

V

G

A

H

R

K

K

L

V

S

I

L

I

L

L

A

A

A

R

I

E

A

S

F

-

G

G

T

L

R

K

V

L

S

D

F

L

D

A

D

D

V

L

Q

P

L

I

E

R

G

S

I

L

-

V

-

P

-

E

-

P

-

L

-

K

G

G

Q

C

I

T

S

S

I

V

D

E

I

F

-

M

-

E

-

K

-

L

-

P

R

Q

H

Y

A

D

G

G

D

D

L

L

-

A

-

K

-

E

-

R

-

L

-

D

-

A

-

E

-

N

-

S

G

G

F

E

R

V

I

L

K

R

L

Y

L

V

G

G

V

V

A

V

Q

D

-

A

V

V

S

N

G

Q

R

K

G

G

-

T

L

V

E

E

Q

L

R

R

M

R

T

-

P

-

C

-

L

-

V

Y

P

K

A

K

D

E

S

H

P

P

L

F

G

A

Q

Q

L

L

Q

A

G

G

G

S

T

D

N

N

M

I

V

I

V

A

L

F

E

T

G

T

D

T

A

R

V

Y

G

K

Q

D

-

H

-

P

I

L

V

I

L

V

R

R

G

G

P

P

G

G

A

A

G

G

E

A

G

Q

P

V

T

T

A

A

S

G

A

G

V

I

M

F

G

S

D

D

V

I

I

L

D

R

L

L

A

A

R

S

G

Y

L

L

R

G

L

A

P

P

T

S

Sites not aligning to the query:

441 I→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
443 Q→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
522 I→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.
524 Q→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.

O94671 Homoserine dehydrogenase; HDH; HSD; EC 1.1.1.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
28% identity, 28% coverage: 96:215/428 of query aligns to 112:239/376 of O94671

query
sites
O94671

L

L

A

S

A

K

G

K

K

I

D

N

V

I

V

A

T

T

A

P

N

N

K

K

A

K

L

A

L

F

A

S

H

A

H

S

G

N

-

D

-

V

-

Y

Q

Q

A

N

L

I

A

I

E

K

M

A

A

S

E

K

A

E

A

S

G

G

L

A

A

L

I

L

R

M

F

H

E

E

A

A

A

S

V

V

A

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

A

T

L

L

T

K

E

E

G

L

L

I

A

A

-

T

G

G

N

D

Q

E

M

I

R

I

R

K

V

I

M

E

G

G

V

I

M

F

N

S

G

G

T

T

C

L

N

S

Y

Y

I

I

L

F

T

N

R

V

M

W

E

S

T

P

A

N

G

G

L

K

P

K

-

G

-

T

-

A

-
x
S

Y

F

D

S

H

D

V

I

F

V

E

K

E

I

A

A

R

K

Q

Q

L

N

G

G

Y

Y

L

T

E

E

A

P

D

D

P

P

N

R

L

D

D

D

V

L

G

N

G

G

I

M

D

D

A

V

G

A

H

R

K

K

L

V

S

T

L

I

L

L

A

S

A

R

I

I

A

A

S201 (vs. gap) modified: Phosphoserine

1tveA Homoserine dehydrogenase in complex with 4-(4-hydroxy-3- isopropylphenylthio)-2-isopropylphenol (see paper)
28% identity, 27% coverage: 99:215/428 of query aligns to 108:230/358 of 1tveA

query
sites
1tveA

G

G

K

I

D

S

V

I

V

A

T

T

A

P

N

N

K
|
K

A

K

L

A

L

F

A

S

H

S

H

D

-

L

-

A

-

T

G

W

Q

K

A

A

L

L

A

F

E

S

M

N

A

K

E

P

A

T

A

N

G

G

L

F

A

-

I

V

R

Y

F

H

E

E

A

A

A

T

V

V

A

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

A

F

L

L

T

R

E

E

G

I

L

I

-

Q

A

T

G

G

N

D

Q

E

M

V

R

E

R

K

V

I

M

E

G

G

V

I

M

F

N

S

G

G

T
|
T

C

L

N

S

Y

Y

I

I

L

F

T

N

R

E

M

F

E

S

T

T

A

S

-

Q

-

A

-

N

G

D

L

V

P

K

Y

F

D

S

H

D

V

V

F

V

E

K

E

V

A

A

R

K

Q

K

L

L

G

G

Y

Y

L

T

E

E

A

P

D

D

P

P

N

R

L

D

D
|
D

V

L

G

N

G

G

I

L

D
|
D

A

V

G

A

H

R

K
|
K

L

V

S

T

L

I

L

V

A

G

A

R

I

I

A

S

active site: D218 (= D203), K222 (= K207)
binding 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol: K116 (= K107), T175 (= T163), D213 (= D198), D218 (= D203)

Sites not aligning to the query:

binding 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol: 93

1q7gA Homoserine dehydrogenase in complex with suicide inhibitor complex NAD-5-hydroxy-4-oxonorvaline (see paper)
28% identity, 27% coverage: 99:215/428 of query aligns to 108:230/358 of 1q7gA

query
sites
1q7gA

G

G

K

I

D

S

V

I

V

A

T

T

A
x
P

N

N

K
|
K

A

K

L

A

L

F

A

S

H

S

H

D

-

L

-

A

-

T

G

W

Q

K

A

A

L

L

A

F

E

S

M

N

A

K

E

P

A

T

A

N

G

G

L

F

A

-

I

V

R

Y

F

H

E

E

A
|
A

A

T

V

V

A

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

A

F

L

L

T

R

E

E

G

I

L

I

-

Q

A

T

G

G

N

D

Q

E

M

V

R

E

R

K

V

I

M

E

G

G

V

I

M

F

N
x
S

G

G

T

T

C

L

N

S

Y

Y

I

I

L

F

T

N

R

E

M

F

E

S

T

T

A

S

-

Q

-

A

-

N

G

D

L

V

P

K

Y

F

D

S

H

D

V

V

F

V

E

K

E

V

A

A

R

K

Q

K

L

L

G

G

Y

Y

L

T

E

E

A

P

D

D

P

P

N

R

L

D

D

D

V

L

G

N

G

G

I

L

D
|
D

A

V

G

A

H

R

K
|
K

L

V

S

T

L

I

L

V

A

G

A

R

I

I

A

S

active site: D218 (= D203), K222 (= K207)
binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: P114 (≠ A105), K116 (= K107), A143 (= A132), S173 (≠ N161), K222 (= K207)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: 13, 14, 15, 39, 91, 92, 93, 97, 338, 343

1ebuD Homoserine dehydrogenase complex with NAD analogue and l-homoserine (see paper)
28% identity, 27% coverage: 99:215/428 of query aligns to 108:230/358 of 1ebuD

query
sites
1ebuD

G

G

K

I

D

S

V

I

V

A

T

T

A

P

N

N

K
|
K

A

K

L

A

L

F

A

S

H

S

H

D

-

L

-

A

-

T

G

W

Q

K

A

A

L

L

A

F

E

S

M

N

A

K

E

P

A

T

A

N

G

G

L

F

A

-

I

V

R

Y

F

H

E

E

A

A

A

T

V

V

A

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

A

F

L

L

T

R

E

E

G

I

L

I

-

Q

A

T

G

G

N

D

Q

E

M

V

R

E

R

K

V

I

M

E

G

G

V

I

M

F

N

S

G

G

T

T

C

L

N

S

Y

Y

I

I

L

F

T

N

R

E

M

F

E

S

T

T

A

S

-

Q

-

A

-

N

G

D

L

V

P

K

Y

F

D

S

H

D

V

V

F

V

E

K

E

V

A

A

R

K

Q

K

L

L

G

G

Y

Y

L

T

E

E

A

P

D

D

P

P

N

R

L

D

D

D

V

L

G

N

G

G

I

L

D
|
D

A

V

G

A

H

R

K
|
K

L

V

S

T

L

I

L

V

A

G

A

R

I

I

A

S

active site: D218 (= D203), K222 (= K207)
binding 3-aminomethyl-pyridinium-adenine-dinucleotide: K116 (= K107)

Sites not aligning to the query:

binding 3-aminomethyl-pyridinium-adenine-dinucleotide: 11, 12, 13, 14, 15, 39, 40, 91, 93, 343

1ebfA Homoserine dehydrogenase from s. Cerevisiae complex with NAD+ (see paper)
28% identity, 27% coverage: 99:215/428 of query aligns to 108:230/358 of 1ebfA

query
sites
1ebfA

G

G

K

I

D

S

V

I

V

A

T

T

A
x
P

N

N

K

K

A

K

L

A

L

F

A

S

H

S

H

D

-

L

-

A

-

T

G

W

Q

K

A

A

L

L

A

F

E

S

M

N

A

K

E

P

A

T

A

N

G

G

L

F

A

-

I

V

R

Y

F

H

E

E

A

A

A

T

V

V

A

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

A

F

L

L

T

R

E

E

G

I

L

I

-

Q

A

T

G

G

N

D

Q

E

M

V

R

E

R

K

V

I

M

E

G

G

V

I

M

F

N

S

G

G

T

T

C

L

N

S

Y

Y

I

I

L

F

T

N

R

E

M

F

E

S

T

T

A

S

-

Q

-

A

-

N

G

D

L

V

P

K

Y

F

D

S

H

D

V

V

F

V

E

K

E

V

A

A

R

K

Q

K

L

L

G

G

Y

Y

L

T

E

E

A

P

D

D

P

P

N

R

L

D

D

D

V

L

G

N

G

G

I

L

D
|
D

A

V

G

A

H

R

K
|
K

L

V

S

T

L

I

L

V

A

G

A

R

I

I

A

S

active site: D218 (= D203), K222 (= K207)
binding nicotinamide-adenine-dinucleotide: P114 (≠ A105)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 10, 12, 13, 14, 15, 39, 40, 92, 93

Query Sequence

>WP_011841410.1 NCBI__GCF_000015985.1:WP_011841410.1
MAAPLRLGIAGLGTVGIGVVKIIQRHADLIAARAGRPVIITAVCARDRSKNRDADLSGYA
WETDAVALAQRADIDVFVEVMGGSEGAARASTEAALAAGKDVVTANKALLAHHGQALAEM
AEAAGLAIRFEAAVAGGIPVIKALTEGLAGNQMRRVMGVMNGTCNYILTRMETAGLPYDH
VFEEARQLGYLEADPNLDVGGIDAGHKLSLLAAIAFGTRVSFDDVQLEGIGQISIDDIRH
AGDLGFRIKLLGVAQVSGRGLEQRMTPCLVPADSPLGQLQGGTNMVVLEGDAVGQIVLRG
PGAGEGPTASAVMGDVIDLARGLRLPTFGRPATSLVEAVPAKVAAPAPWYLRMTLLDKPG
ALAKIATALGEAGISIDRMRQYGHEGGHAPVLIVTHKASRDDISHAISRFGATGVLVGEP
VAIRIEEV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory