SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011841694.1 NCBI__GCF_000015985.1:WP_011841694.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P05041 Aminodeoxychorismate synthase component 1; ADC synthase; ADCS; 4-amino-4-deoxychorismate synthase component 1; EC 2.6.1.85 from Escherichia coli (strain K12) (see 4 papers)
45% identity, 70% coverage: 104:370/381 of query aligns to 182:445/453 of P05041

query
sites
P05041

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N

E258 (= E181) mutation to A: The reaction is extremely slow.; mutation to D: The reaction is extremely slow.
K274 (= K198) mutation to A: Absence of covalent intermediate. Addition of ammonia allows the formation of the covalent intermediate and shows that ammonia can replace the function of K-274. Reduced catalytic efficiency.; mutation to R: Absence of covalent intermediate.; mutation to R: Reduced catalytic efficiency.
G275 (= G199) mutation to S: Catalytically inactive for both the glutamine-dependent and ammonia-dependent reactions and fails to interact with PabA.
R311 (= R235) mutation to K: Catalytically active in the NH3-dependent, but inactive for the glutamine-dependent reactions and fails to complex with PabA.
R316 (= R240) mutation to H: Catalytically inactive for both the glutamine-dependent and ammonia-dependent reactions and fails to interact with PabA.
S322 (= S246) mutation to T: Complete loss of aminodeoxychorismate synthase activity.
H339 (≠ Q263) mutation to W: Catalytically inactive for both the glutamine-dependent and ammonia-dependent reactions and fails to interact with PabA.

Sites not aligning to the query:

36 binding L-tryptophan

8hx9A Crystal structure of 4-amino-4-deoxychorismate synthase from streptomyces venezuelae with chorismate (see paper)
44% identity, 68% coverage: 111:370/381 of query aligns to 365:625/632 of 8hx9A

query
sites
8hx9A

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binding (3R,4R)-3-[(1-carboxyethenyl)oxy]-4-hydroxycyclohexa-1,5-diene-1-carboxylic acid: I453 (≠ M197), K454 (= K198), G455 (= G199), T456 (= T200), M547 (≠ V291), Y570 (= Y314), R590 (= R334), V603 (= V348), G604 (= G349), G605 (= G350), A606 (≠ G351), E619 (= E364), K623 (= K368)
binding tryptophan: P419 (≠ G163), Y420 (≠ Q164), G421 (= G165), L574 (≠ M318), G575 (= G319)

Sites not aligning to the query:

binding magnesium ion: 134, 135
binding tryptophan: 189, 190, 191, 199, 201

8hx8A Crystal structure of 4-amino-4-deoxychorismate synthase from streptomyces venezuelae co-crystallized with chorismate (see paper)
44% identity, 68% coverage: 111:370/381 of query aligns to 404:664/673 of 8hx8A

query
sites
8hx8A

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binding magnesium ion: E521 (= E226), E655 (= E361), E658 (= E364)
binding tryptophan: P458 (≠ G163), Y459 (≠ Q164), G460 (= G165), G614 (= G319)

Sites not aligning to the query:

binding magnesium ion: 175, 177
binding tryptophan: 231, 232, 233, 241, 243

1k0eA The crystal structure of aminodeoxychorismate synthase from formate grown crystals (see paper)
41% identity, 70% coverage: 104:370/381 of query aligns to 180:429/437 of 1k0eA

query
sites
1k0eA

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active site: E256 (= E181), K272 (= K198), E286 (= E226), H323 (≠ Q263), S350 (= S290), W374 (≠ Y314), R394 (= R334), G410 (= G351), E423 (= E364), K427 (= K368)
binding tryptophan: P238 (≠ G163), F239 (≠ Q164), S240 (≠ G165)

Sites not aligning to the query:

binding tryptophan: 32, 33, 34, 41, 44

P28820 Aminodeoxychorismate synthase component 1; ADC synthase; ADCS; 4-amino-4-deoxychorismate synthase component 1; EC 2.6.1.85 from Bacillus subtilis (strain 168) (see paper)
40% identity, 67% coverage: 115:368/381 of query aligns to 202:452/470 of P28820

query
sites
P28820

Y

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L

S

E

T

T

R

R

P

P

M

I

K
x
A

G

G

T

T

A

R

P

S

R

R

D

G

P

K

D

T

P

N

A

E

R

E

D

D

A

E

E

A

L

L

A

A

K

N

I

E

L

L

G

I

S

H

S

N

E

E

K

K

G

E

R

R

A

A

E

E

N

H

L

V

M

M

I

L

V

V

D

D

L

L

L

E

R

R

N

N

D

D

I

L

G

G

R

R

I

V

A

S

E

R

L

Y

G

G

S

S

V

V

R

R

V

V

P

N

E

E

L

F

F

M

A

A

I

I

E

E

S

K

Y

Y

A

S

T

H

V

V

H

M

Q

H

M

I

V

V

S

S

R

N

V

V

T

Q

G

G

R

E

L

L

I

Q

G

D

W

G

P

Y

G

D

L

A

S

V

G

D

L

I

P

H

A

A

L

V

F

F

P

P

C

G

G

G

S

T

V

I

T

T

G

G

A

A

P

P

K

K

I

V

R

R

A

T

M

M

E

E

I

I

G

E

E

E

L

L

E

E

P

P

F

T

P

R

R

R

G

G

L

L

Y

Y

C

T

G

G

A

S

M

I

G

G

W

W

M

F

A

G

P

Y

D

N

G

H

R

D

A

L

A

Q

F

F

N

N

V

I

A

V

I

I

R

R

T

T

L

I

R

Y

L

A

F

-

P

T

G

G

E

Q

L

A

R

F

L

M

D

Q

V

S

G

G

G

A

G

G

I

V

V

V

Q

I

D

D

S

S

T

V

A

P

P

K

G

H

E

E

W

Y

E

K

E

E

A

S

L

F

W

K

K

K

A283 (≠ K198) mutation to I: Complete loss of aminodeoxychorismate synthase activity.; mutation to K: Absence of covalent intermediate.; mutation to V: Complete loss of aminodeoxychorismate synthase activity.

7pi1DDD Aminodeoxychorismate synthase component 1
40% identity, 67% coverage: 115:368/381 of query aligns to 195:445/459 of 7pi1DDD

query
sites
7pi1DDD

Y

F

D

S

A

Q

A

A

F

V

G

E

R

K

L

I

M

K

A

Q

Y

Y

I

I

A

A

A

S

G

G

D

D

C

V

Y

F

Q

Q

V

V

N

N

L

L

T

S

F

I

P

R

L

Q

D

S

G

Q

R

S

L

L

E

S

A

V

G

-

S

H

A

P

L

Y

G

Q

L

I

Y

Y

G

K

A

T

L

L

R

R

A

E

R

V

Q

N

P

P

V

S

G
x
P

Q
x
Y

G
x
M

A

A

F

Y

C

L

D

E

L

T

G

P

G

D

P

F

V

Q

A

I

I

I

S

C

A

G

S

S

P

P

E

E

L

L

F

L

F

-

D

-

C

V

D

S

A

K

E

K

G

G

R

K

I

L

-

L

S

E

T

T

R

R

P

P

M

I

K

A

G

G

T

T

A

R

P

S

R

R

D

G

P

K

D

T

P

N

A

E

R

E

D

D

A

E

E

A

L

L

A

A

K

N

I

E

L

L

G

I

S

H

S

N

E

E

K

K

G

E

R

R

A

A

E

E

N

H

L

V

M

M

I

L

V

V

D

D

L

L

L

E

R

R

N

N

D

D

I

L

G

G

R

R

I

V

A

S

E

R

L

Y

G

G

S

S

V

V

R

R

V

V

P

N

E

E

L

F

F

M

A

A

I

I

E

E

S

K

Y

Y

A

S

T

H

V

V

H

M

Q

H

M

I

V

V

S

S

R

N

V

V

T

Q

G

G

R

E

L

L

I

Q

G

D

W

G

P

Y

G

D

L

A

S

V

G

D

L

I

P

H

A

A

L

V

F

F

P

P

C

G

G

G

S

T

V

I

T

T

G

G

A

A

P

P

K

K

I

V

R

R

A

T

M

M

E

E

I

I

G

E

E

E

L

L

E

E

P

P

F

T

P

R

R

R

G

G

L

L

Y

Y

C

T

G

G

A

S

M

I

G

G

W

W

M

F

A

G

P

Y

D

N

G

H

R

D

A

L

A
x
Q

F

F

N
|
N

V

I

A

V

I

I

R

R

T

T

L

I

R

Y

L

A

F

-

P

T

G

G

E

Q

L

A

R

F

L

M

D

Q

V

S

G

G

G

A

G
|
G

I

V

V

V

Q

I

D

D

S

S

T

V

A

P

P

K

G

H

E
|
E

W

Y

E

K

E

E

A

S

L

F

W

K

K

K

binding magnesium ion: G428 (= G351), E438 (= E361)
binding tryptophan: P242 (≠ G163), Y243 (≠ Q164), M244 (≠ G165), Q406 (≠ A328), N408 (= N330)

Sites not aligning to the query:

binding tryptophan: 33, 34, 35, 39, 41

1k0gA The crystal structure of aminodeoxychorismate synthase from phosphate grown crystals (see paper)
39% identity, 70% coverage: 104:370/381 of query aligns to 182:412/420 of 1k0gA

query
sites
1k0gA

A

S

P

D

F

W

R

Q

P

S

R

N

I

M

A

T

R

R

A

E

D

Q

Y

Y

D

G

A

E

A

K

F

F

G

R

R

Q

L

V

M

Q

A

E

Y

Y

I

L

A

H

A

S

G

G

D

D

C

C

Y

Y

Q

Q

V

V

N

N

L

L

T

A

F

Q

P

R

L

F

D

H

G

A

R

T

L

Y

-

S

-

G

-

D

E

E

A

W

G

Q

S

A

A

F

L

L

G

Q

L

L

Y

N

G

Q

A

A

L

N

R

R

A

A

R

-

Q

-

P
|
P

V
x
F

G
x
S

Q

-

G

-

A

A

F

F

C

L

D

R

L

L

G

E

G

Q

P

G

V

A

A

I

I

L

S

S

A

L

S

S

P

P

E
|
E

L

R

F

F

F

I

D

L

C

C

D

D

A

-

E

N

G

S

R

E

I

I

S

Q

T

T

R

R

P

P

M

I

K
|
K

G

D

T

-

A

-

P

-

R

-

D

-

P

-

D

-

P

-

A

-

R

-

D

-

A

-

E

-

L

-

A

-

K

-

I

-

L

-

G

-

S

-

E

-

K

-

G

-

R

R

A

A

E
|
E

N

N

L

L

M

M

I

I

V

V

D

D

L

L

L

M

R

R

N

N

D

D

I

I

G

G

R

R

I

V

A

A

E

V

L

A

G

G

S

S

V

V

R

K

V

V

P

P

E

E

L

L

F

F

A

V

I

-

E

-

S

-

Y

-

A

-

T

-

V

-

H

-

Q

-

M

L

V

V

S

S

R

T

V

I

T

T

G

A

R

Q

L

L

I

P

G

E

W

Q

P

L

G

H

L

A

S

S

G

D

L

L

P

R

A

A

L

A

F

F

P

P

C

G

G

G

S
|
S

V

I

T

T

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

E

I

I

G
x
D

E

E

L

L

E

E

P

P

F

Q

P
x
R

R

R

G

N

L

A

Y
x
W

C

C

G

G

A

S

M

I

G

G

W

Y

M

L

A

S

P

F

D

C

G

G

R

N

A

M

A

D

F

T

N

S

V

I

A

T

I

I

R
|
R

T

T

L

L

R

T

L

A

F

I

P

-

G

N

G

G

E

Q

L

I

R

F

L

C

D

S

V

A

G

G

G
|
G

I

I

V

V

Q

A

D

D

S

S

T

Q

A

E

P

E

G

A

E

E

W

Y

E

Q

E
|
E

A

T

L

F

W

D

K
|
K

T

V

R

N

active site: E258 (= E181), K274 (= K198), E278 (= E226), S333 (= S290), W357 (≠ Y314), R377 (= R334), G393 (= G351), E406 (= E364), K410 (= K368)
binding phosphate ion: D347 (≠ G304), R353 (≠ P310)
binding tryptophan: P240 (= P161), F241 (≠ V162), S242 (≠ G163)

Sites not aligning to the query:

binding phosphate ion: 113, 116
binding tryptophan: 34, 35, 36, 43, 44, 46

1k0gB The crystal structure of aminodeoxychorismate synthase from phosphate grown crystals (see paper)
39% identity, 70% coverage: 104:370/381 of query aligns to 182:409/415 of 1k0gB

query
sites
1k0gB

A

S

P

D

F

W

R

Q

P

S

R

N

I

M

A

T

R

R

A

E

D

Q

Y

Y

D

G

A

E

A

K

F

F

G

R

R

Q

L

V

M

Q

A

E

Y

Y

I

L

A

H

A

S

G

G

D

D

C

C

Y

Y

Q

Q

V

V

N

N

L

L

T

A

F

Q

P

R

L

F

D

H

G

A

R

T

L

Y

-

S

-

G

-

D

E

E

A

W

G

Q

S

A

A

F

L

L

G

Q

L

L

Y

N

G

Q

A

A

L

N

R

R

A

A

R

-

Q

-

P
|
P

V
x
F

G

S

Q

-

G

-

A

A

F

F

C

L

D

R

L

L

G

E

G

Q

P

G

V

A

A

I

I

L

S

S

A

L

S

S

P

P

E
|
E

L

R

F

F

F

I

D

L

C

C

D

D

A

-

E

N

G

S

R

E

I

I

S

Q

T

T

R

R

P

P

M

I

K
|
K

G

G

T

-

A

-

P

-

R

-

D

-

P

-

D

-

P

-

A

-

R

-

D

-

A

-

E

-

L

-

A

-

K

-

I

-

L

-

G

-

S

-

E

-

K

-

G

-

R

-

A

A

E
|
E

N

N

L

L

M

M

I

I

V

V

D

D

L

L

L

M

R

R

N

N

D

D

I

I

G

G

R

R

I

V

A

A

E

V

L

A

G

G

S

S

V

V

R

K

V

V

P

P

E

E

L

L

F

-

A

-

I

-

E

-

S

-

Y

-

A

-

T

-

V

-

H

-

Q

-

M

L

V

V

S

S

R

T

V

I

T

T

G

A

R

Q

L

L

I

P

G

E

W

Q

P

L

G

H

L

A

S

S

G

D

L

L

P

R

A

A

L

A

F

F

P

P

C

G

G

G

S
|
S

V

I

T

T

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

E

I

I

G
x
D

E

E

L

L

E

E

P

P

F

Q

P
x
R

R

R

G

N

L

A

Y
x
W

C

C

G

G

A

S

M

I

G

G

W

Y

M

L

A

S

P

F

D

C

G

G

R

N

A

M

A

D

F

T

N

S

V

I

A

T

I

I

R
|
R

T

T

L

L

R

T

L

A

F

I

P

-

G

N

G

G

E

Q

L

I

R

F

L

C

D

S

V

A

G

G

G
|
G

I

I

V

V

Q

A

D

D

S

S

T

Q

A

E

P

E

G

A

E

E

W

Y

E

Q

E
|
E

A

T

L

F

W

D

K
|
K

T

V

R

N

active site: E258 (= E181), K274 (= K198), E277 (= E226), S330 (= S290), W354 (≠ Y314), R374 (= R334), G390 (= G351), E403 (= E364), K407 (= K368)
binding phosphate ion: D344 (≠ G304), R350 (≠ P310)
binding tryptophan: P240 (= P161), F241 (≠ V162)

Sites not aligning to the query:

binding phosphate ion: 112, 113, 116
binding tryptophan: 34, 35, 36, 43, 44, 45, 46

A0QX93 Anthranilate synthase component 1; AS; ASI; EC 4.1.3.27 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
38% identity, 70% coverage: 106:370/381 of query aligns to 242:509/524 of A0QX93

query
sites
A0QX93

F

H

R

R

P

A

R

Q

I

R

A

T

R

M

A

E

D

E

Y

Y

D

T

A

E

A

I

F

V

G

D

R

K

L

L

M

V

A

G

Y

D

I

I

A

E

A

A

G

G

D

E

C

A

Y

F

Q

Q

V

V

-

V

-

P

N

S

L

Q

T

R

F

F

P

E

L

M

D

D

G

-

R

-

L

-

E

T

A

A

G

A

S

D

A

P

L

L

G

D

L

V

Y

Y

G

R

A

I

L

L

R

R

A

V

R

T

Q

N

P

P

V

S

G

P

Q

Y

G

M

A

Y

F

L

C

L

D

N

L

I

-

P

-

D

-

A

-

D

G

G

P

L

-

D

-

F

V

S

A

I

I

V

S

G

A

S

S

S

P

P

E

E

L

A

F

L

F

V

D

T

C

V

D

-

A

K

E

D

G

G

R

R

I

A

S

T

T

T

R

H

P

P

M

I

K

A

G

G

T

T

A

R

P

W

R

R

D

G

P

A

D

T

P

E

A

E

R

E

D

D

A

V

E

L

L

L

A

E

K
|
K

I

E

L

L

G

L

S

A

S

D

E

E

K

K

G

E

R

R

A

A

E

E

N

H

L

L

M

M

I

L

V

V

D

D

L

L

L

G

R

R

N

N

D

D

I

L

G

G

R

R

I

V

A

C

E

R

L

P

G

G

S

T

V

V

R

R

V

V

P

D

E

D

L

Y

F

S

A

H

I

I

E

E

S

R

Y

Y

A

S

T

H

V

V

H

M

Q

H

M

L

V

V

S

S

R

T

V

V

T

T

G

G

R

E

L

L

I

A

G

E

W

D

P

K

G

T

L

A

S

L

G

D

L

A

L

V

P

T

A

A

L

C

F

F

P

P

C

A

G

G

S

T

V

L

T

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

E

I

L

I

I

G

E

E

E

L

V

E

E

P

K

F

T

P

R

R

R

G

G

L

L

Y

Y

C

G

G

G

A

V

M

V

G

G

W

Y

M

L

A

D

P

F

D

A

G

G

R

N

A

A

A

D

F

F

N

A

V

I

A

A

I

I

R

R

T

T

L

A

R

-

L

L

F

M

P

R

G

N

G

G

E

T

L

A

R

Y

L

V

D

Q

V

A

G

G

I

V

V

V

Q

A

D

D

S

S

T

N

A

G

P

P

G

Y

E

E

W

Y

E

T

E

E

A

A

L

A

W

N

K

K

T

A

R

R

K355 (= K215) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

7bvdA Anthranilate synthase component i (trpe)[mycolicibacterium smegmatis]
38% identity, 70% coverage: 106:370/381 of query aligns to 221:484/499 of 7bvdA

query
sites
7bvdA

F

H

R

R

P

A

R

Q

I

R

A

T

R

M

A

E

D

E

Y

Y

D

T

A

E

A

I

F

V

G

D

R

K

L

L

M

V

A

G

Y

D

I

I

A

E

A

A

G

G

D

E

C

A

Y

F

Q
|
Q

V

V

-

V

-

P

N

S

L

Q

T

R

F

F

P

E

L

M

D

D

G

-

R

-

L

-

E

T

A

A

G

A

S

D

A

P

L

L

G

D

L

V

Y

Y

G

R

A

I

L

L

R

R

A

V

R

T

Q

N

P

P

V

S

G

P

Q

Y

G

M

A

Y

F

L

C

L

D

N

L

I

-

P

-

D

-

A

-

D

G

G

P

L

-

D

-

F

V

S

A

I

I

V

S

G

A

S

S

S

P

P

E
|
E

L

A

F

L

F

V

D

T

C

V

D

-

A

K

E

D

G

G

R

R

I

A

S

T

T

T

R

H

P

P

M

I

K
x
A

G

G

T

T

A

R

P

W

R

R

D

E

P

-

D

-

P

-

A

-

R

E

D

D

A

V

E

L

L

L

A

E

K

K

I

E

L

L

G

L

S

A

S

D

E

E

K

K

G

E

R

R

A

A

E
|
E

N

H

L

L

M

M

I

L

V

V

D

D

L

L

L

G

R

R

N

N

D

D

I

L

G

G

R

R

I

V

A

C

E

R

L

P

G

G

S

T

V

V

R

R

V

V

P

D

E

D

L

Y

F

S

A

H

I

I

E

E

S

R

Y

Y

A

S

T

H

V

V

H

M

Q
x
H

M

L

V

V

S

S

R

T

V

V

T

T

G

G

R

E

L

L

I

A

G

E

W

D

P

K

G

T

L

A

S

L

G

D

L

A

L

V

P

T

A

A

L

C

F

F

P

P

C

A

G

G

S
x
T

V

L

T

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

E

I

L

I

I

G

E

E

E

L

V

E

E

P

K

F

T

P

R

R

R

G

G

L

L

Y
|
Y

C

G

G

G

A

V

M

V

G

G

W

Y

M

L

A

D

P

F

D

A

G

G

R

N

A

A

A

D

F

F

N

A

V

I

A

A

I

I

R
|
R

T

T

L

A

R

-

L

L

F

M

P

R

G

N

G

G

E

T

L

A

R

Y

L

V

D

Q

V

A

G

G

G
|
G

I

V

V

V

Q

A

D

D

S

S

T

N

A

G

P

P

G

Y

E

E

W

Y

E

T

E
|
E

A

A

L

A

W

N

K
|
K

T

A

R

R

active site: Q248 (= Q133), E301 (= E181), A317 (≠ K198), E341 (= E226), H378 (≠ Q263), T405 (≠ S290), Y429 (= Y314), R449 (= R334), G465 (= G351), E478 (= E364), K482 (= K368)

Sites not aligning to the query:

binding pyruvic acid: 93, 94, 100

O94582 Probable anthranilate synthase component 1; Anthranilate synthase component I; EC 4.1.3.27 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
35% identity, 69% coverage: 107:370/381 of query aligns to 207:468/489 of O94582

query
sites
O94582

R

K

P

S

R

N

I

V

A

G

R

E

A

D

D

G

Y

Y

D

K

A

A

A

F

F

V

G

S

R

N

L

L

M

K

A

E

Y

H

I

I

A

F

A

N

G

G

D

D

C

I

Y

F

Q

Q

V

A

N

V

L

P

T

S

F

Q

P

R

L

I

D

A

G

R

R

R

L

T

E

D

A

L

G

-

S

H

A

P

L

F

G

N

L

L

Y

Y

G

R

A

H

L

L

R

R

A

T

R

V

Q

N

P

P

V

S

G

P

Q

Y

G

M

A

F

F

Y

C

I

D

H

L

C

G

D

P

F

V

D

A

I

I

I

S

G

A

A

S

S

P

P

E

E

L

L

F

L

F

V

D

K

C

S

D

E

A

-

E

H

G

G

R

R

I

I

S

I

T

N

R

H

P

P

M

I

K

A

G

G

T

T

A

V

P

P

R

R

D

G

P

K

D

T

P

K

A

E

R

E

D

D

A

E

E

A

L

Y

A

A

K

K

I

D

L

L

G

L

S

A

S

S

E

V

K

K

G

D

R

R

A

A

E

E

N

H

L

V

M

M

I

L

V

V

D

D

L

L

L

A

R

R

N

N

D

D

I

V

G

S

R

R

I

V

A

C

E

D

L

L

G

D

S

T

V

T

R

S

V

V

P

D

E

K

L

L

F

M

A

T

I

I

E

E

S

K

Y

F

A

S

T

H

V

V

H

Q

Q

H

M

L

V

V

S

S

R

Q

V

V

T

S

G

G

R

V

L

L

I

R

G

P

W

D

P

K

G

T

L

R

S

F

G

D

L

A

L

F

P

R

A

S

L

I

F

F

P

P

C

A

G

G

S

T

V

V

T
x
S

G

G

A
x
S

P

P

K

K

I

V

R

R

A

A

M

I

E

Q

I

L

I

V

G

Y

E

G

L

L

E

E

P

K

F

E

P

K

R

R

G

G

L

I

Y

Y

C

A

G

G

A

A

M

V

G

G

-

R

W

W

M

G

A

Y

P

E

D

D

G

D

R

N

A

M

A

D

F

T

N

C

V

I

A

A

I

I

R

R

T

T

L

M

R

-

L

V

F

Y

P

K

G

D

G

G

E

T

L

V

R

Y

L

L

D

Q

V

A

G

G

I

I

V

V

Q

F

D

D

S

S

T

D

A

E

P

Q

G

D

E

E

W

Y

E

V

E

E

A

T

L

L

W

N

K

K

T

L

R

R

S390 (≠ T292) modified: Phosphoserine
S392 (≠ A294) modified: Phosphoserine

Sites not aligning to the query:

488 modified: Phosphoserine

5cwaA Structure of anthranilate synthase component i (trpe) from mycobacterium tuberculosis with inhibitor bound (see paper)
38% identity, 67% coverage: 114:370/381 of query aligns to 229:488/505 of 5cwaA

query
sites
5cwaA

D

E

Y

Y

D

G

A

A

A

I

F

V

G

E

R

Y

L

L

M

V

A

D

Y

Q

I

I

A

A

G

G

D

E

C

A

Y

F

Q
|
Q

V

V

-

V

-

P

N

S

L

Q

T

R

F

F

P

E

L

M

D

D

G

T

R

D

L

V

E

D

A

P

G

-

S

-

A

-

L

I

G

D

L

V

Y

Y

G

R

A

I

L

L

R

R

A

V

R

T

Q

N

P

P

V

S

G

P

Q

Y

G

M

A

Y

F

L

C

L

D

Q

L

V

-

P

-

N

-

S

G

D

G

G

P

A

V

V

A

D

I

F

S

S

-

I

-

V

-

G

A

S

S

S

P

P

E
|
E

L

A

F

L

F

V

D

T

C

V

D

H

A

-

E

E

G

G

R

W

I

A

S

T

T

T

R

H

P

P

M

I

K
x
A

G

G

T

T

A

R

P

W

R

R

D

G

P

R

D

T

P

D

A

D

R

E

D

D

A

V

E

L

L

L

A

E

K

K

I

E

L

L

G

L

S

A

S

D

E

D

K

K

G

E

R

R

A

A

E
|
E

N

H

L

L

M

M

I

L

V

V

D

D

L

L

L

G

R

R

N

N

D

D

I

L

G

G

R

R

I

V

A

C

E

T

L

P

G

G

S

T

V

V

R

R

V

V

P

E

E

D

L

Y

F

S

A

H

I

I

E

E

S

R

Y

Y

A

S

T

H

V

V

H

M

Q
x
H

M

L

V

V

S

S

R

T

V

V

T

T

G

G

R

K

L

L

I

G

G

E

W

G

P

R

G

T

L

A

S

L

G

D

L

A

L

V

P

T

A

A

L

C

F

F

P

P

C

A

G

G

S
x
T

V

L

T

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

E

I

L

I

I

G

E

E

E

L

V

E

E

P

K

F

T

P

R

R

R

G

G

L

L

Y
|
Y

C

G

G

G

A

V

M

V

G

G

W

Y

M

L

A

D

P

F

D

A

G

G

R

N

A

A

A

D

F

F

N

A

V

I

A

A

I
|
I

R
|
R

T

T

L

A

R

-

L

L

F

M

P

R

G

N

G

G

E

T

L

A

R

Y

L

V

D

Q

V
x
A

G
|
G

G

G

G
|
G

I

V

V

V

Q

A

D

D

S

S

T

N

A

G

P

S

G

Y

E

E

W

Y

E

N

E
|
E

A

A

L

R

W

N

K
|
K

T

A

R

R

active site: Q248 (= Q133), E301 (= E181), A317 (≠ K198), E345 (= E226), H382 (≠ Q263), T409 (≠ S290), Y433 (= Y314), R453 (= R334), G469 (= G351), E482 (= E364), K486 (= K368)
binding 3-{[(1Z)-1-carboxyprop-1-en-1-yl]oxy}-2-hydroxybenzoic acid: Y433 (= Y314), I452 (= I333), A466 (≠ V348), G467 (= G349), K486 (= K368)

Q94GF1 Anthranilate synthase alpha subunit 1, chloroplastic; OsASA1; EC 4.1.3.27 from Oryza sativa subsp. japonica (Rice) (see paper)
34% identity, 69% coverage: 107:368/381 of query aligns to 283:559/577 of Q94GF1

query
sites
Q94GF1

R

K

P

S

R

S

I

M

A

S

R

R

A

E

D

D

Y

Y

D

K

A

K

A

A

F

V

G

V

R

Q

L

A

M

K

A

E

Y

H

I

I

A

L

A

A

G

G

D

D

C

I

Y

F

Q

Q

V

V

N

V

L

L

T

S

F

Q

P

R

L

F

D

E

G

R

R

R

L

T

E

F

A

A

G

-

S
x
D

A

P

L

F

G

E

L

V

Y

Y

G

R

A

A

L

L

R

R

A

I

R

V

Q

N

P

P

V

S

G

P

Q

Y

G

M

A

A

F

Y

C

L

D

Q

L

A

G

R

G

G

P

C

V

I

A

L

I

V

S

A

A

S

S

S

P

P

E

E

L

I

F

L

F

T

D

R

C

V

D

E

A

K

E

R

G

T

R

-

I

I

S

V

T

N

R

R

P

P

M

L

K

A

G

G

T

T

A

I

P

R

R

R

D

G

P

K

D

S

P

K

A

A

R

E

D

D

A

K

E

V

L

L

A

E

K

Q

I

L

L

L

G

L

S

S

S

D

E

G

K

K

G

Q

R

C

A

A

E

E

N

H

L

I

M

M

I

L

V

V

D

D

L

L

L

G

R

R

N

N

D

D

I

V

G

G

R

K

I

V

A

S

E

K

L

P

G

G

S

S

V

V

R

K

V

V

P

E

E

K

L

L

F

M

A

N

I

V

E

E

S

R

Y

Y

A

S

T

H

V

V

H

M

Q

H

M

I

V

S

S

S

R

T

V

V

T

T

G

G

R

E

L

L

I

R

G

D

W

D

P

L

G

T

L

C

S

W

G

D

L

A

L

L

P

R

A

A

L

A

F

L

P

P

C

V

G

G

S

T

V

V

T

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

E

I

L

I

I

G

D

E

Q

L

M

E

E

P

G

F

K

P

M

R

R

G

G

L

P

Y

Y

C

S

G

G

A

G

M

F

G

G

W

G

M

V

A

S

P

F

D

R

G

G

R

D

A

M

A

D

F

I

N

A

V

L

A

A

I

L

R

R

T

T

L

I

R

-

L

V

F

F

P

P

G

T

G

G

E

S

-

R

-

F

-

D

-

T

-

M

-

Y

-

S

-

Y

-

T

-

D

-

K

-

N

-

A

-

R

-

Q

-

E

-

W

-

V

L

A

R

H

L

L

D

Q

V

A

G

G

G

A

G

G

I

I

V

V

Q

A

D

D

S

S

T

K

A

P

P

D

G

D

E

E

W

H

E

Q

E

E

A

C

L

L

W

N

K

K

D323 (≠ S148) mutation to N: Insensitive to feedback inhibition by tryptophan.

P32068 Anthranilate synthase alpha subunit 1, chloroplastic; Anthranilate synthase component 1-1; Anthranilate synthase component I-1; Protein A-METHYL TRYPTOPHAN RESISTANT 1; Protein JASMONATE-INDUCED DEFECTIVE LATERAL ROOT 1; Protein TRYPTOPHAN BIOSYNTHESIS 5; Protein WEAK ETHYLENE INSENSITIVE 2; EC 4.1.3.27 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
36% identity, 60% coverage: 114:340/381 of query aligns to 308:531/595 of P32068

query
sites
P32068

D

E

Y

Y

D

K

A

E

A

A

F

V

G

V

R

K

L

A

M

K

A

E

Y

H

I

I

A

L

A

A

G

G

D

D

C

I

Y

F

Q

Q

V

I

N

V

L

L

T

S

F

Q

P

R

L

F

D

E

G

R

R

R

L

T

E

F

A

A

G

-

S
x
D

A

P

L

F

G

E

L

V

Y

Y

G

R

A

A

L

L

R

R

A

V

R

V

Q

N

P

P

V

S

G

P

Q

Y

G

M

A

G

F

Y

C

L

D

Q

L

A

G

R

G

G

P

C

V

I

A

L

I

V

S

A

A

S

S

S

P

P

E

E

L

I

F

L

F

T

D

K

C

V

D

-

A

K

E

Q

G

N

R

K

I

I

S

V

T

N

R

R

P

P

M

L

K

A

G

G

T

T

A

S

P

K

R

R

D

G

P

K

D

N

P

E

A

V

R

E

D

D

A

K

E

R

L

L

A

E

K

K

I

E

L

L

G

L

S

E

S

N

E

E

K

K

G

Q

R

C

A

A

E

E

N

H

L

I

M

M

I

L

V

V

D

D

L

L

L

G

R

R

N

N

D

D

I

V

G

G

R

K

I

V

A

T

E

K

L

Y

G

G

S

S

V

V

R

K

V

V

P

E

E

K

L

L

F

M

A

N

I

I

E

E

S

R

Y

Y

A

S

T

H

V

V

H

M

Q

H

M

I

V

S

S

S

R

T

V

V

T

T

G

G

R

E

L

L

I

Q

G

D

W

G

P

L

G

T

L

C

S

W

G

D

L

V

L

L

P

R

A

A

L

A

F

L

P

P

C

V

G

G

S

T

V

V

T

S

G

G

A

A

P

P

K

K

I

V

R

K

A

A

M

M

E

E

I

L

I

I

G

D

E

E

L

L

E

E

P

P

F

T

P

R

R

R

G

G

L

P

Y

Y

C

S

G

G

A

G

M

F

G

G

W

G

M

V

A

S

P

F

D

T

G

G

R

D

A

M

A

D

F

I

N

A

V

L

A

S

I

L

R

R

T

T

L

I

R

-

L

V

F

F

P

P

D341 (≠ S148) mutation to N: In trp5-1; insensitive to feedback inhibition by tryptophan and resistance to the herbicide 6-methylanthranilate.

1i1qA Structure of the cooperative allosteric anthranilate synthase from salmonella typhimurium (see paper)
33% identity, 67% coverage: 116:370/381 of query aligns to 238:500/512 of 1i1qA

query
sites
1i1qA

D

D

A

D

A

A

F

F

G

G

-

A

-

V

-

R

R

Q

L

L

M

Q

A

K

Y

A

I

I

A

R

A

A

G

G

D

E

C

I

Y

F

Q
|
Q

V

V

N

V

L

P

T

S

F

R

P

R

L

F

D

S

G

-

R

-

L

L

E

P

A

C

G

P

S

S

A

P

L

L

G

A

L

A

Y

Y

G

Y

A

V

L

L

R

K

A

K

R

S

Q

N

P

P

V

S

G
x
P

Q
x
Y

G
x
M

A

F

F

F

C

M

D

Q

L

D

G

N

G

D

P

F

V

T

A

L

I

F

S

G

A

A

S

S

P

P

E
|
E

L

S

F

S

F

L

D

K

C

Y

D

D

A

A

E

A

G

S

R

R

-

Q

I

I

S

E

T

I

R

Y

P

P

M

I

K
x
A

G

G

T

T

A

R

P

P

R

R

-

G

-

R

-

A

-

D

-

G

D

T

P

L

D

D

P

R

A

D

R

L

D

D

A

S

E

R

L

I

A

E

K

L

I

D

L

M

G

R

S

T

S

D

E

H

K

K

G

E

R

L

A

S

E
|
E

N

H

L

L

M

M

I

L

V

V

D

D

L

L

L

A

R

R

N

N

D

D

I

L

G

A

R

R

I

I

A

C

E

T

L

P

G

G

S

S

V

R

R

Y

V

V

P

A

E

D

L

L

F

T

A

K

I

V

E

D

S

R

Y

Y

A

S

T

Y

V

V

H

M

Q
x
H

M

L

V

V

S

S

R

R

V

V

T

V

G

G

R

E

L

L

I

R

G

H

W

D

P

L

G

D

L

A

S

L

G

H

L

A

L

Y

P

R

A

A

L

C

F

M

P

N

C

M

G

G

S
x
T

V

L

T

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

Q

I

L

I

I

G

A

E

D

L

A

E

E

P

G

F

Q

P

R

R

R

G

G

L

S

Y
|
Y

C

G

G

G

A

A

M

V

G
|
G

W

Y

M

F

A

T

P

A

D

H

G

G

R

D

A

L

A

D

F

T

N
x
C

V

I

A

V

I

I

R
|
R

T

S

L

A

R

-

L

L

F

V

P

E

G

N

G

G

E

I

L

A

R

T

L

V

D

Q

V

A

G

G

G

A

G
|
G

I

I

V

V

Q

L

D

D

S

S

T

V

A

P

P

Q

G

S

E

E

W

A

E

D

E
|
E

A

T

L

R

W

N

K
|
K

T

A

R

R

active site: Q259 (= Q133), E305 (= E181), A323 (≠ K198), E357 (= E226), H394 (≠ Q263), T421 (≠ S290), Y445 (= Y314), R465 (= R334), G481 (= G351), E494 (= E364), K498 (= K368)
binding tryptophan: P287 (≠ G163), Y288 (≠ Q164), M289 (≠ G165), G450 (= G319), C461 (≠ N330)

Sites not aligning to the query:

binding tryptophan: 34, 35, 36, 46

P00898 Anthranilate synthase component 1; AS; ASI; EC 4.1.3.27 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
33% identity, 67% coverage: 116:370/381 of query aligns to 242:504/520 of P00898

query
sites
P00898

D

D

A

D

A

A

F

F

G

G

-

A

-

V

-

R

R

Q

L

L

M

Q

A

K

Y

A

I

I

A

R

A

A

G

G

D

E

C

I

Y

F

Q

Q

V

V

N

V

L

P

T

S

F

R

P

R

L

F

D

S

G

-

R

-

L

L

E

P

A

C

G

P

S

S

A

P

L

L

G

A

L

A

Y

Y

G

Y

A

V

L

L

R

K

A

K

R

S

Q
x
N

P
|
P

V

S

G

P

Q

Y

G
x
M

A
x
F

F

F

C

M

D

Q

L

D

G

N

G

D

P

F

V

T

A

L

I

F

S
x
G

A

A

S

S

P

P

E

E

L

S

F

S

F

L

D

K

C

Y

D

D

A

A

E

A

G

S

R

R

-

Q

I

I

S

E

T

I

R

Y

P

P

M

I

K

A

G

G

T

T

A

R

P

P

R

R

-

G

-

R

-

A

-

D

-

G

D

T

P

L

D

D

P

R

A

D

R

L

D

D

A

S

E

R

L

I

A

E

K

L

I

D

L

M

G

R

S

T

S

D

E

H

K

K

G

E

R

L

A

S

E

E

N

H

L

L

M

M

I

L

V

V

D

D

L

L

L

A

R

R

N

N

D

D

I

L

G

A

R

R

I

I

A

C

E

T

L

P

G

G

S

S

V

R

R

Y

V

V

P

A

E

D

L

L

F

T

A

K

I

V

E

D

S

R

Y

Y

A

S

T

Y

V

V

H

M

Q

H

M

L

V

V

S

S

R
|
R

V

V

T

V

G

G

R

E

L

L

I

R

G

H

W

D

P

L

G

D

L

A

S

L

G

H

L

A

L

Y

P

R

A

A

L

C

F

M

P

N

C

M

G

G

S

T

V

L

T

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

Q

I

L

I

I

G

A

E

D

L

A

E

E

P

G

F

Q

P

R

R

R

G

G

L

S

Y

Y

C

G

G

G

A

A

M

V

G

G

W

Y

M

F

A

T

P

A

D

H

G
|
G

R

D

A

L

A

D

F

T

N
x
C

V

I

A

V

I

I

R

R

T

S

L

A

R

-

L

L

F

V

P

E

G

N

G

G

E

I

L

A

R

T

L

V

D

Q

V

A

G

G

G

A

G

G

I

I

V

V

Q

L

D

D

S

S

T

V

A

P

P

Q

G

S

E

E

W

A

E

D

E

E

A

T

L

R

W

N

K

K

T

A

R

R

N288 (≠ Q160) mutation to D: Decrease in feedback control by tryptophan.
P289 (= P161) mutation to L: Decrease in feedback control by tryptophan.
M293 (≠ G165) mutation to T: Complete loss of feedback control by tryptophan.
F294 (≠ A166) mutation to L: Decrease in feedback control by tryptophan.
G305 (≠ S177) mutation to S: Decrease in feedback control by tryptophan.
R402 (= R267) mutation to W: Almost no change in feedback control by tryptophan.
G460 (= G325) mutation to D: Almost no change in feedback control by tryptophan.
C465 (≠ N330) mutation to Y: Complete loss of feedback control by tryptophan. 4-fold decrease of affinity binding for chorismate.

Sites not aligning to the query:

39 E→K: Complete loss of feedback control by tryptophan.
40 binding L-tryptophan; S→F: Complete loss of feedback control by tryptophan.
41 A→V: Decrease in feedback control by tryptophan.
50 binding L-tryptophan
128 R→H: Almost no change in feedback control by tryptophan.
174 C→Y: Almost no change in feedback control by tryptophan.
515 H→Y: Almost no change in feedback control by tryptophan.

P00897 Anthranilate synthase component 1; AS; ASI; EC 4.1.3.27 from Serratia marcescens (see paper)
31% identity, 67% coverage: 114:370/381 of query aligns to 243:503/519 of P00897

query
sites
P00897

D

E

Y

Y

D

G

A

A

A

V

F

V

G

S

R

E

L

L

M

Q

A

E

Y

A

I

I

A

R

A

Q

G

G

D

E

C

I

Y

F

Q

Q

V

V

-

V

-

P

-

S

-

R

N

R

L

F

T

S

F

L

P

P

L

-

D

-

G

-

R

-

L

-

E

-

A

C

G

P

S

A

A

P

L

L

G

G

L

P

Y

Y

G

Q

A

T

L

L

R

K

A

D

R

N

Q

N

P

P

V

S

G
x
P

Q
x
Y

G
x
M

A

F

F

F

C

M

D

Q

L

D

G

D

P

F

V

T

A

L

I

F

S

G

A

A

S

S

P

P

E

E

L

S

F

A

F

L

D

K

C

Y

D

D

A

A

E

G

G

N

R

R

-

Q

I

I

S

E

T

I

R

Y

P

P

M

I

K

A

G

G

T

T

A

R

P

P

R

R

-

G

-

R

-

A

-

D

-

G

D

S

P

L

D

D

P

L

A

D

R

L

D

D

A

S

E

R

L

I

A

E

K

L

I

E

L

M

G

R

S

T

S

D

E

H

K

K

G

E

R

L

A

A

E
|
E

N

H

L

L

M

M

I

L

V

V

D

D

L

L

L

A

R

R

N

N

D

D

I

L

G

A

R

R

I

I

A

C

E

Q

L

A

G

G

S

S

V

R

R

Y

V

V

P

A

E

D

L

L

F

T

A

K

I

V

E

D

S

R

Y

Y

A

S

T

F

V

V

H

M

Q

H

M

L

V

V

S

S

R

R

V

V

T

V

G

G

R

T

L

L

I

R

G

A

W

D

P

L

G

D

L

V

S

L

G

H

L

A

L

Y

P

Q

A

A

L

C

F

M

P

N

C

M

G

G

S

T

V

L

T

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

Q

I

L

I

I

G

A

E

A

L

L

E

R

P

S

F

T

P

R

R

R

G

G

L

S

Y

Y

C

G

G

G

A

R

M

V

G

G

W

Y

M

F

A

T

P

A

D

H

G

R

R

H

A

L

A

D

F

T

N

C

V

I

A

V

I

I

R

R

T

S

L

A

R

Y

L

V

F

-

P

E

G

D

G

G

E

H

L

R

R

T

L

V

D

Q

V

A

G

G

G

A

G

G

I

V

V

V

Q

Q

D

D

S

S

T

I

A

R

P

R

G

R

E

E

W

A

E

D

E
|
E

A

T

L

R

W

N

K

K

T

A

R

R

PYM 290:292 (≠ GQG 163:165) binding L-tryptophan
E360 (= E226) binding Mg(2+)
E497 (= E364) binding Mg(2+)

Sites not aligning to the query:

39 binding L-tryptophan

1i7sA Anthranilate synthase from serratia marcescens in complex with its end product inhibitor l-tryptophan (see paper)
31% identity, 67% coverage: 114:370/381 of query aligns to 235:495/511 of 1i7sA

query
sites
1i7sA

D

E

Y

Y

D

G

A

A

A

V

F

V

G

S

R

E

L

L

M

Q

A

E

Y

A

I

I

A

R

A

Q

G

G

D

E

C

I

Y

F

Q
|
Q

V

V

-

V

-

P

-

S

-

R

N

R

L

F

T

S

F

L

P

P

L

-

D

-

G

-

R

-

L

-

E

-

A

C

G

P

S

A

A

P

L

L

G

G

L

P

Y

Y

G

Q

A

T

L

L

R

K

A

D

R

N

Q

N

P

P

V

S

G
x
P

Q
x
Y

G
x
M

A

F

F

F

C

M

D

Q

L

D

G

D

P

F

V

T

A

L

I

F

S

G

A

A

S

S

P

P

E
|
E

L

S

F

A

F

L

D

K

C

Y

D

D

A

A

E

G

G

N

R

R

-

Q

I

I

S

E

T

I

R

Y

P

P

M

I

K
x
A

G

G

T

T

A

R

P

P

R

R

-

G

-

R

-

A

-

D

-

G

D

S

P

L

D

D

P

L

A

D

R

L

D

D

A

S

E

R

L

I

A

E

K

L

I

E

L

M

G

R

S

T

S

D

E

H

K

K

G

E

R

L

A

A

E
|
E

N

H

L

L

M

M

I

L

V

V

D

D

L

L

L

A

R

R

N

N

D

D

I

L

G

A

R

R

I

I

A

C

E

Q

L

A

G

G

S

S

V

R

R

Y

V

V

P

A

E

D

L

L

F

T

A

K

I

V

E

D

S

R

Y

Y

A

S

T

F

V

V

H

M

Q
x
H

M

L

V

V

S

S

R

R

V

V

T

V

G

G

R

T

L

L

I

R

G

A

W

D

P

L

G

D

L

V

S

L

G

H

L

A

L

Y

P

Q

A

A

L

C

F

M

P

N

C

M

G

G

S
x
T

V

L

T

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

Q

I

L

I

I

G

A

E

A

L

L

E

R

P

S

F

T

P

R

R

R

G

G

L

S

Y
|
Y

C

G

G

G

A

R

M
x
V

G
|
G

W

Y

M

F

A

T

P

A

D

V

G

R

R

N

A

L

A
x
D

F

T

N
x
C

V

I

A

V

I

I

R
|
R

T

S

L

A

R

Y

L

V

F

-

P

E

G

D

G

G

E

H

L

R

R

T

L

V

D

Q

V

A

G

G

G

A

G
|
G

I

V

V

V

Q

Q

D

D

S

S

T

I

A

P

P

E

G

R

E

E

W

A

E

D

E
|
E

A

T

L

R

W

N

K
|
K

T

A

R

R

active site: Q254 (= Q133), E300 (= E181), A318 (≠ K198), E352 (= E226), H389 (≠ Q263), T416 (≠ S290), Y440 (= Y314), R460 (= R334), G476 (= G351), E489 (= E364), K493 (= K368)
binding tryptophan: P282 (≠ G163), Y283 (≠ Q164), M284 (≠ G165), V444 (≠ M318), G445 (= G319), D454 (≠ A328), C456 (≠ N330)

Sites not aligning to the query:

binding tryptophan: 35, 36, 37

1i7qA Anthranilate synthase from s. Marcescens (see paper)
31% identity, 67% coverage: 114:370/381 of query aligns to 241:501/517 of 1i7qA

query
sites
1i7qA

D

E

Y

Y

D

G

A

A

A

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G

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E

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A

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Y

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Q

V

V

-

V

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P

-

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-

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N

R

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F

L

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P

L

-

D

-

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-

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-

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-

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-

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C

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A

A

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L

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G

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Y

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A

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A

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N

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E

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Y

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A

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G

N

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-

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x
A

G

G

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T

A

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R

R

-

G

-

R

-

A

-

D

-

G

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L

A

D

R

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E

N

H

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D

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T

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A

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P

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K

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R

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I

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A

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A

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Y

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G

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G

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A

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x
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active site: Q260 (= Q133), E306 (= E181), A324 (≠ K198), E358 (= E226), H395 (≠ Q263), T422 (≠ S290), Y446 (= Y314), R466 (= R334), G482 (= G351), E495 (= E364), K499 (= K368)
binding magnesium ion: E358 (= E226), E495 (= E364)
binding pyruvic acid: Y446 (= Y314), I465 (= I333), R466 (= R334), A479 (≠ V348), G480 (= G349), K499 (= K368)

5jxzA A low magnesium structure of the isochorismate synthase, entc (see paper)
31% identity, 67% coverage: 112:365/381 of query aligns to 109:360/373 of 5jxzA

query
sites
5jxzA

R

Q

A

T

D

T

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F

D

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A

Q

A

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F

V

G

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R

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A

A

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L

I

T

A

A

A

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K

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V

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V

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L

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F

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D

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I

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A

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G

-

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L

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E

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x
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I

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H

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L

L

L

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A

L

L

L

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x
A

V

L

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G

A

F

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P

K

H

I

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R

A

A

A

M

T

E

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I

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I

I

G

A

E

E

L

L

E

E

P

P

F

F

P

D

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R

G

E

L

L

Y
x
F

C

G

G

G

A

I

M

V

G

G

W

W

M

C

A

D

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S

D

E

G

G

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I

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R

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G

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G

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active site: K130 (≠ Q133), E180 (= E181), A196 (≠ K198), E224 (= E226), H259 (≠ Q263), A286 (≠ S290), F310 (≠ Y314), R330 (= R334), G346 (= G351), E359 (= E364)
binding (5S,6S)-5-[(1-carboxyethenyl)oxy]-6-hydroxycyclohexa-1,3-diene-1-carboxylic acid: L195 (≠ M197), G197 (= G199), S198 (≠ T200), E224 (= E226), A286 (≠ S290), I329 (= I333), R330 (= R334), A343 (≠ V348), G344 (= G349), E359 (= E364)
binding (3R,4R)-3-[(1-carboxyethenyl)oxy]-4-hydroxycyclohexa-1,5-diene-1-carboxylic acid: E180 (= E181), L195 (≠ M197), A196 (≠ K198), G197 (= G199), E224 (= E226), A286 (≠ S290), I329 (= I333), R330 (= R334), G344 (= G349), A345 (≠ G350), E359 (= E364)
binding magnesium ion: E224 (= E226), E359 (= E364)

Sites not aligning to the query:

Query Sequence

>WP_011841694.1 NCBI__GCF_000015985.1:WP_011841694.1
MFLCENGPRSGPALFREPVETVIVEEPAALAPALARLDALRAEGFWIAGTVAYEAGLVFE
PRLARLMPPQRSEPLLAFGAFRAPLPAGDLLAQAAAEAGEVRLAPFRPRIARADYDAAFG
RLMAYIAAGDCYQVNLTFPLDGRLEAGSALGLYGALRARQPVGQGAFCDLGGPVAISASP
ELFFDCDAEGRISTRPMKGTAPRDPDPARDAELAKILGSSEKGRAENLMIVDLLRNDIGR
IAELGSVRVPELFAIESYATVHQMVSRVTGRLIGWPGLSGLLPALFPCGSVTGAPKIRAM
EIIGELEPFPRGLYCGAMGWMAPDGRAAFNVAIRTLRLFPGGELRLDVGGGIVQDSTAPG
EWEEALWKTRFAELPTTPTCG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory