SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011842229.1 NCBI__GCF_000015985.1:WP_011842229.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9R4E4 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; CP4 EPSP synthase; EC 2.5.1.19 from Agrobacterium sp. (strain CP4) (see paper)
58% identity, 99% coverage: 1:442/445 of query aligns to 1:447/455 of Q9R4E4

query
sites
Q9R4E4

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S29 (= S29) binding 3-phosphoshikimate
R33 (= R33) binding 3-phosphoshikimate
A100 (≠ G101) mutation to G: Confers sensitivity to glyphosate allowing glyphosate to bind in its extended, inhibitory conformation.
S173 (= S174) binding 3-phosphoshikimate
A174 (= A175) binding 3-phosphoshikimate
Q175 (= Q176) binding 3-phosphoshikimate
D326 (= D326) binding 3-phosphoshikimate
K353 (= K353) binding 3-phosphoshikimate

2pqcA Cp4 epsps liganded with (r)-phosphonate tetrahedral reaction intermediate analog (see paper)
58% identity, 98% coverage: 7:442/445 of query aligns to 2:442/445 of 2pqcA

query
sites
2pqcA

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active site: K23 (= K28), S24 (= S29), D50 (= D55), N93 (= N99), R123 (= R129), D321 (= D326), E349 (= E354), H399 (= H399), R400 (= R400), T426 (= T426)
binding [3r-[3a,4a,5b(r*)]]-5-(1-carboxy-1-phosphonoethoxy)-4-hydroxy-3-(phosphonooxy)-1-cyclohexene-1-carboxylic acid: K23 (= K28), S24 (= S29), R28 (= R33), T96 (= T102), R123 (= R129), S168 (= S174), Q170 (= Q176), D321 (= D326), K348 (= K353), E349 (= E354), R352 (= R357), R400 (= R400)

2pqbA Cp4 epsps liganded with (r)-difluoromethyl tetrahedral intermediate analog (see paper)
58% identity, 98% coverage: 7:442/445 of query aligns to 2:442/445 of 2pqbA

query
sites
2pqbA

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A

L

C

N

G

G

V

V

R

D

I

C

E

D

E

E

D

G

E

E

D

T

T

S

L

L

I

V

V

V

H

R

G

G

M

R

G

P

R

D

V

G

P

K

G

G

-

L

-

G

-

N

-

A

-

S

G

G

A

A

T

A

C

V

A

A

T

T

H

H

L

L

D

D

H
|
H

R
|
R

I

I

A

A

M

M

S

S

F

F

L

L

V

V

L

M

G

G

M

L

A

V

A

S

E

E

A

N

P

P

V

V

T

T

V

V

D

D

G

A

S

T

P

M

I

I

A

A

T
|
T

S

S

F

F

P

P

A

E

F

F

T

M

D

D

L

L

M

M

T

A

G

G

L

L

G

G

A

A

D

K

L

I

active site: K23 (= K28), S24 (= S29), D50 (= D55), N93 (= N99), R123 (= R129), D321 (= D326), E349 (= E354), H399 (= H399), R400 (= R400), T426 (= T426)
binding (3r,4s,5r)-5-[(1r)-1-carboxy-2,2-difluoro-1-(phosphonooxy)ethoxy]-4-hydroxy-3-(phosphonooxy)cyclohex-1-ene-1-carboxylic acid: K23 (= K28), S24 (= S29), R28 (= R33), A95 (≠ G101), T96 (= T102), R123 (= R129), S168 (= S174), Q170 (= Q176), D321 (= D326), K348 (= K353), E349 (= E354), R352 (= R357), R400 (= R400)

2ggaA Cp4 epsp synthase liganded with s3p and glyphosate (see paper)
58% identity, 98% coverage: 7:442/445 of query aligns to 2:442/445 of 2ggaA

query
sites
2ggaA

A

S

Q

R

P

P

M

A

T

T

A

A

R

R

R

K

S

S

G

S

P

G

L

L

K

S

G

G

R

T

A

V

E

R

I

I

P

P

G

G

D

D

K
|
K

S
|
S

I

I

S

S

H

H

R
|
R

A

S

L

F

I

M

L

F

G

G

A

G

M

L

A

A

V

S

G

G

E

E

T

T

Q

R

I

I

T

T

G

G

L

L

E

E

G

G

Q

E

D
|
D

V

V

L

I

D

N

T

T

A

G

K

K

A

A

M

M

R

Q

A

A

F

M

G

G

A

A

E

R

V

I

I

R

Q

K

H

E

G

G

P

D

G

-

A

T

W

W

S

I

V

I

H

D

G

G

V

V

G

G

V

N

G

G

F

L

A

L

E

A

P

P

A

E

E

A

V

P

I

L

D

D

C

F

G

G

N
|
N

S
x
A

G
x
A

T
|
T

G

G

V

C

R

R

L

L

V

T

M

M

G

G

A

L

M

V

A

G

T

V

S

Y

P

D

L

F

T

D

A

S

T

T

F

F

T

I

G

G

D

D

A

A

S

S

L

L

R

T

K

K

R
|
R

P

P

M

M

G

G

R

R

V

V

T

L

D

N

P

P

L

L

A

R

L

E

F

M

G

G

T

V

R

Q

A

V

Y

K

G

S

R

E

K

D

G

G

G

D

R

R

L

L

P

P

M

V

T

T

L

L

V

R

G

G

A

P

A

K

D

T

P

P

V

T

P

P

V

I

R

T

Y

Y

T

R

V

V

P

P

M

M

P

A

S
|
S

A
|
A

Q
|
Q

V

V

K

K

S

S

A

A

V

V

L

L

A

A

G

G

L

L

N

N

A

T

P

P

G

G

Q

I

T

T

V

T

V

V

I

I

E

E

R

P

E

I

A

M

T

T

R
|
R

D

D

H

H

S

T

E

E

R

K

M

M

L

L

R

Q

G

G

F

F

G

G

A

A

E

N

L

L

S

T

V

V

E

E

T

T

G

D

P

A

E

D

G

G

-

V

Q

R

I

T

I

I

T

R

L

L

T

E

G

G

Q

R

P

G

E

K

L

L

R

T

P

G

Q

Q

T

V

V

I

A

D

V

V

P

P

R

G

D

D

P

P

S

S

A

T

A

A

F

F

P

P

V

L

C

V

A

A

L

L

I

L

V

V

E

P

G

G

S

S

E

D

I

V

L

T

V

I

P

L

G

N

V

V

S

L

R

M

N

N

P

P

T

T

R

R

D

T

G

G

L

L

Y

I

V

L

T

T

L

L

L

Q

E

E

M

M

G

G

A

A

D

D

I

I

A

E

F

V

E

I

N

N

E

P

R

R

E

L

E

A

G

G

E

E

P

D

V

V

A

A

D

D

L

L

R

R

V

V

R

R

A

S

S

S

A

T

L

L

K

K

G

G

V

V

E

T

V

V

P

P

P

E

E

D

R

R

A

A

P

P

S

S

M

M

I

I

D
|
D

E

E

Y

Y

P

P

I

I

L

L

S

A

V

V

V

A

A

A

F

F

A

A

E

E

G

G

S

A

T

T

I

V

M

M

R

N

G

G

V

L

K

E

E

E

L

L

R

R

V

V

K
|
K

E
|
E

S

S

D

D

R
|
R

I

L

D

S

A

A

M

V

A

A

R

N

G

G

L

L

E

K

A

L

C

N

G

G

V

V

R

D

I

C

E

D

E

E

D

G

E

E

D

T

T

S

L

L

I

V

V

V

H

R

G

G

M

R

G

P

R

D

V

G

P

K

G

G

-

L

-

G

-

N

-

A

-

S

G

G

A

A

T

A

C

V

A

A

T

T

H

H

L

L

D

D

H
|
H

R
|
R

I

I

A

A

M

M

S

S

F

F

L

L

V

V

L

M

G

G

M

L

A

V

A

S

E

E

A

N

P

P

V

V

T

T

V

V

D

D

G

A

S

T

P

M

I

I

A

A

T
|
T

S

S

F

F

P

P

A

E

F

F

T

M

D

D

L

L

M

M

T

A

G

G

L

L

G

G

A

A

D

K

L

I

active site: K23 (= K28), S24 (= S29), D50 (= D55), N93 (= N99), R123 (= R129), D321 (= D326), E349 (= E354), H399 (= H399), R400 (= R400), T426 (= T426)
binding glyphosate: K23 (= K28), A94 (≠ S100), A95 (≠ G101), T96 (= T102), R123 (= R129), D321 (= D326), E349 (= E354), R352 (= R357), R400 (= R400)
binding shikimate-3-phosphate: S24 (= S29), R28 (= R33), S168 (= S174), A169 (= A175), Q170 (= Q176), R195 (= R201), D321 (= D326), K348 (= K353)

2gg6A Cp4 epsp synthase liganded with s3p (see paper)
58% identity, 98% coverage: 7:442/445 of query aligns to 2:442/445 of 2gg6A

query
sites
2gg6A

A

S

Q

R

P

P

M

A

T

T

A

A

R

R

R

K

S

S

G

S

P

G

L

L

K

S

G

G

R

T

A

V

E

R

I

I

P

P

G

G

D

D

K
|
K

S
|
S

I

I

S

S

H

H

R
|
R

A

S

L

F

I

M

L

F

G

G

A

G

M

L

A

A

V

S

G

G

E

E

T

T

Q

R

I

I

T

T

G

G

L

L

E

E

G

G

Q

E

D
|
D

V

V

L

I

D

N

T

T

A

G

K

K

A

A

M

M

R

Q

A

A

F

M

G

G

A

A

E

R

V

I

I

R

Q

K

H

E

G

G

P

D

G

-

A

T

W

W

S

I

V

I

H

D

G

G

V

V

G

G

V

N

G

G

F

L

A

L

E

A

P

P

A

E

E

A

V

P

I

L

D

D

C

F

G

G

N
|
N

S

A

G

A

T
|
T

G

G

V

C

R

R

L

L

V

T

M

M

G

G

A

L

M

V

A

G

T

V

S

Y

P

D

L

F

T

D

A

S

T

T

F

F

T

I

G

G

D

D

A

A

S

S

L

L

R

T

K

K

R
|
R

P

P

M

M

G

G

R

R

V

V

T

L

D

N

P

P

L

L

A

R

L

E

F

M

G

G

T

V

R

Q

A

V

Y

K

G

S

R

E

K

D

G

G

G

D

R

R

L

L

P

P

M

V

T

T

L

L

V

R

G

G

A

P

A

K

D

T

P

P

V

T

P

P

V

I

R

T

Y

Y

T

R

V

V

P

P

M

M

P

A

S
|
S

A

A

Q
|
Q

V

V

K

K

S

S

A

A

V

V

L

L

A

A

G

G

L

L

N

N

A

T

P

P

G

G

Q

I

T

T

V

T

V

V

I

I

E

E

R

P

E

I

A

M

T

T

R

R

D

D

H

H

S

T

E

E

R

K

M

M

L

L

R

Q

G

G

F

F

G

G

A

A

E

N

L

L

S

T

V

V

E

E

T

T

G

D

P

A

E

D

G

G

-

V

Q

R

I

T

I

I

T

R

L

L

T

E

G

G

Q

R

P

G

E

K

L

L

R

T

P

G

Q

Q

T

V

V

I

A

D

V

V

P

P

R

G

D

D

P

P

S

S

A

T

A

A

F

F

P

P

V

L

C

V

A

A

L

L

I

L

V

V

E

P

G

G

S

S

E

D

I

V

L

T

V

I

P

L

G

N

V

V

S

L

R

M

N

N

P

P

T

T

R

R

D

T

G

G

L

L

Y

I

V

L

T

T

L

L

L

Q

E

E

M

M

G

G

A

A

D

D

I

I

A

E

F

V

E

I

N

N

E

P

R

R

E

L

E

A

G

G

E

E

P

D

V

V

A

A

D

D

L

L

R

R

V

V

R

R

A

S

S

S

A

T

L

L

K

K

G

G

V

V

E

T

V

V

P

P

P

E

E

D

R

R

A

A

P

P

S

S

M

M

I

I

D
|
D

E

E

Y

Y

P

P

I

I

L

L

S

A

V

V

V

A

A

A

F

F

A

A

E

E

G

G

S

A

T

T

I

V

M

M

R

N

G

G

V

L

K

E

E

E

L

L

R

R

V

V

K
|
K

E
|
E

S

S

D

D

R

R

I

L

D

S

A

A

M

V

A

A

R

N

G

G

L

L

E

K

A

L

C

N

G

G

V

V

R

D

I

C

E

D

E

E

D

G

E

E

D

T

T

S

L

L

I

V

V

V

H

R

G

G

M

R

G

P

R

D

V

G

P

K

G

G

-

L

-

G

-

N

-

A

-

S

G

G

A

A

T

A

C

V

A

A

T

T

H

H

L

L

D

D

H
|
H

R
|
R

I

I

A

A

M

M

S

S

F

F

L

L

V

V

L

M

G

G

M

L

A

V

A

S

E

E

A

N

P

P

V

V

T

T

V

V

D

D

G

A

S

T

P

M

I

I

A

A

T
|
T

S

S

F

F

P

P

A

E

F

F

T

M

D

D

L

L

M

M

T

A

G

G

L

L

G

G

A

A

D

K

L

I

active site: K23 (= K28), S24 (= S29), D50 (= D55), N93 (= N99), R123 (= R129), D321 (= D326), E349 (= E354), H399 (= H399), R400 (= R400), T426 (= T426)
binding shikimate-3-phosphate: S24 (= S29), R28 (= R33), T96 (= T102), S168 (= S174), Q170 (= Q176), D321 (= D326), K348 (= K353)

3slhD 1.70 angstrom resolution structure of 3-phosphoshikimate 1- carboxyvinyltransferase (aroa) from coxiella burnetii in complex with shikimate-3-phosphate and glyphosate
42% identity, 96% coverage: 18:442/445 of query aligns to 13:431/440 of 3slhD

query
sites
3slhD

L

L

K

S

G

G

R

E

A

I

E

C

I

V

P

P

G

G

D

D

K
|
K

S
|
S

I

I

S

S

H

H

R
|
R

A

A

L

V

I

L

L

L

G

A

A

A

M

I

A

A

V

E

G

G

E

Q

T

T

Q

Q

I

V

T

D

G

G

L

F

L

L

E

M

G

G

Q

A

D
|
D

V

N

L

L

D

A

T

M

A

V

K

S

A

A

M

L

R

Q

A

Q

F

M

G

G

A

A

E

S

V

I

-

Q

I

V

Q

I

H

E

G

D

P

E

G

N

A

I

W

L

S

V

V

V

H

E

G

G

V

V

G

G

V

M

G

T

G

G

F

L

A

Q

E

A

P

P

A

P

E

E

V

A

I

L

D

D

C

C

G

G

N
|
N

S

S

G
|
G

T
|
T

G

A

V

I

R

R

L

L

V

L

M

S

G

G

A

L

M

L

A

A

T

G

S

Q

P

P

L

F

T

N

A

T

T

V

F

L

T

T

G

G

D

D

A

S

S

S

L

L

R

Q

K

R

R
|
R

P

P

M

M

G

K

R

R

V

I

T

I

D

D

P

P

L

L

A

T

L

L

F

M

G

G

T

A

R

K

A

-

Y

I

G

D

R

S

K

T

G

G

G

N

R

V

L

P

P

P

M

L

T

K

L

I

V

Y

G

G

A

N

A

P

D

R

P

L

V

T

P

G

V

I

R

H

Y

Y

T

Q

V

L

P

P

M

M

P

A

S
|
S

A

A

Q
|
Q

V

V

K

K

S

S

A

C

V

L

L

L

A

A

G

G

L

L

N

Y

A

A

P

R

G

G

Q

K

T

T

V

C

V

I

I

T

E

E

R

P

E

A

A

P

T

S

R
|
R

D

D

H

H

S

T

E

E

R

R

M

L

L

L

R

K

G

H

F

F

G

H

A

Y

E

T

L

L

S

Q

V

-

E

-

T

-

G

-

P

K

E

D

G

K

Q

Q

I

S

I

I

T

C

L

V

T

S

G

G

Q

G

P

G

E

K

L

L

R

K

P

A

Q

N

T

D

V

I

A

S

V

I

P

P

R

G

D

D

P

I

S

S

A

A

F

F

P

F

V

I

C

V

A

A

L

T

I

I

V

T

E

P

G

G

S

S

E

A

I

I

L

R

V

L

P

C

G

R

V

V

S

G

R

V

N

N

P

P

T

T

R

R

D

L

G

G

L

V

Y

I

V

N

T

L

L

L

L

K

E

M

M

M

G

G

A

A

D

D

I

I

A

E

F

V

E

T

N

H

E

Y

R

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E

E

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K

G

N

G

E

E

E

P

P

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T

A

A

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D

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I

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T

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V

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H

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A

A

R

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L

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L

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-

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R

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I

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A

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F

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A

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L

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S

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L

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active site: K23 (= K28), S24 (= S29), D50 (= D55), N95 (= N99), R125 (= R129), D317 (= D326), E345 (= E354), H388 (= H399), R389 (= R400), T415 (= T426)
binding glyphosate: K23 (= K28), G97 (= G101), T98 (= T102), R125 (= R129), Q171 (= Q176), D317 (= D326), E345 (= E354), R348 (= R357), H388 (= H399), R389 (= R400)
binding shikimate-3-phosphate: S24 (= S29), R28 (= R33), S169 (= S174), Q171 (= Q176), R196 (= R201), D317 (= D326), K344 (= K353)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S24 (= S29), R28 (= R33), T98 (= T102), Q171 (= Q176), R196 (= R201), D317 (= D326), K344 (= K353)

Q83E11 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; EC 2.5.1.19 from Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
42% identity, 96% coverage: 18:442/445 of query aligns to 11:429/438 of Q83E11

query
sites
Q83E11

L

L

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-

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K21 (= K28) binding phosphoenolpyruvate
S22 (= S29) binding 3-phosphoshikimate
R26 (= R33) binding 3-phosphoshikimate
NSGT 93:96 (= NSGT 99:102) Phosphoenolpyruvate
G95 (= G101) binding phosphoenolpyruvate
T96 (= T102) binding phosphoenolpyruvate
R123 (= R129) binding phosphoenolpyruvate
S167 (= S174) binding 3-phosphoshikimate
A168 (= A175) binding 3-phosphoshikimate
Q169 (= Q176) binding 3-phosphoshikimate; binding phosphoenolpyruvate
D315 (= D326) binding 3-phosphoshikimate
K342 (= K353) binding 3-phosphoshikimate
R346 (= R357) binding phosphoenolpyruvate
R387 (= R400) binding phosphoenolpyruvate

4egrA 2.50 angstrom resolution structure of 3-phosphoshikimate 1- carboxyvinyltransferase (aroa) from coxiella burnetii in complex with phosphoenolpyruvate
42% identity, 96% coverage: 18:442/445 of query aligns to 13:427/434 of 4egrA

query
sites
4egrA

L

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A

A

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L

L

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A

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G

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Q

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D

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N

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A

I

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D

D

C

C

G

G

N
|
N

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G

T
|
T

G

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R

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G

G

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P

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L

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H

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|
R

I

I

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A

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A

F

F

L

A

V

V

L

A

G

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A

L

A

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A

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R

D

N

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|
T

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S

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active site: K23 (= K28), S24 (= S29), D50 (= D55), N95 (= N99), R125 (= R129), D313 (= D326), E341 (= E354), H384 (= H399), R385 (= R400), T411 (= T426)
binding phosphoenolpyruvate: K23 (= K28), G97 (= G101), T98 (= T102), R125 (= R129), D313 (= D326), E341 (= E354), R344 (= R357), R385 (= R400)

Q9S400 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; EC 2.5.1.19 from Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) (see paper)
40% identity, 95% coverage: 18:438/445 of query aligns to 10:424/427 of Q9S400

query
sites
Q9S400

L

L

K

H

G

G

R

S

A

I

E

R

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P

P

G

G

D

D

K

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S
|
S

I

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S

S

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H

R
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R

A

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L

I

I

I

L

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F

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G

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E

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-

I

I

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D

G

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A

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G

A

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S

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A

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Q

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K

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-

P

-

E

D

G

G

Q

K

I

K

I

I

T

T

L

V

T

Q

G

G

Q

P

P

Q

E

K

L

L

R

T

P

G

Q

Q

T

K

V

V

A

V

V

V

P

P

R

G

D

D

P

I

S

S

A

A

F

F

P

W

V

L

C

V

A

A

A

G

L

L

I

I

V

A

E

P

G

N

S

S

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R

I

L

L

V

V

L

P

Q

G

N

V

V

S

G

R

I

N

N

P

E

T

T

R

R

D

T

G

G

L

I

Y

I

V

D

T

V

L

I

L

R

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A

M

M

G

G

A

G

D

K

I

L

A

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F

I

E

T

N

E

E

I

R

-

E

-

G

-

E

D

P

P

V

V

A

A

D

K

-

S

-

A

-

T

L

L

R

I

V

V

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A

S

S

S

A

D

L

L

K

K

G

G

V

T

E

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V

I

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G

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A

R

L

A

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P

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R

M

L

I

I

D
|
D

E

E

Y

L

P

P

I

I

L

I

S

A

V

L

A

A

A

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F

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A

A

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Q

G

G

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T

T

I

V

M

I

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D

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A

K

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E

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L

L

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|
K

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S

T

D

D

R

R

I

I

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Q

A

V

M

V

A

A

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A

L

L

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N

A

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G

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A

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T

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A

D

D

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L

M

I

I

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G

G

M

K

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R

A

V

L

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H

G

-

G

G

A

A

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C

V

A

N

T

T

H

F

L

G

D

D

H

H

R

R

I

I

A

G

M

M

S

M

F

T

L

A

V

I

L

A

G

A

-

L

M

L

A

V

A

A

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D

A

G

P

E

V

V

T

E

V

L

D

D

D

R

G

A

S

E

P

A

I

I

A

N

T

T

S

S

F

Y

P

P

A

S

F

F

T

F

D

D

L

D

M

L

T

E

G

S

L

L

S21 (= S29) binding 3-phosphoshikimate
R25 (= R33) binding 3-phosphoshikimate
S166 (= S174) binding 3-phosphoshikimate
A167 (= A175) binding 3-phosphoshikimate
Q168 (= Q176) binding 3-phosphoshikimate
D312 (= D326) binding 3-phosphoshikimate
K339 (= K353) binding 3-phosphoshikimate

1rf6A Structural studies of streptococcus pneumoniae epsp synthase in s3p- glp bound state (see paper)
40% identity, 95% coverage: 18:438/445 of query aligns to 10:424/427 of 1rf6A

query
sites
1rf6A

L

L

K

H

G

G

R

I

A

I

E

R

I

V

P

P

G

G

D

D

K
|
K

S
|
S

I

I

S

S

H

H

R
|
R

A

S

L

I

I

I

L

F

G

G

A

S

M

L

A

A

V

E

G

G

E

E

T

T

Q

K

I

V

T

Y

G

D

L

I

L

L

E

R

G

G

Q

E

D
|
D

V

V

L

L

D

S

T

T

A

M

K

Q

A

V

M

F

R

R

A

D

F

L

G

G

A

V

E

E

V

-

I

I

Q

E

H

D

G

K

P

D

G

G

A

V

W

I

S

T

V

V

H

Q

G

G

V

V

G

G

V

M

G

A

G

G

F

L

A

K

E

A

P

P

A

Q

E

N

V

A

I

L

D

N

C

M

G

G

N
|
N

S

S

G
|
G

T
|
T

G

S

V

I

R

R

L

L

V

I

M

S

G

G

A

V

M

L

A

A

T

G

S

A

P

D

L

F

T

E

A

V

T

E

F

M

T

F

G

G

D

D

A
x
D

S

S

L

L

R

S

K

K

R
|
R

P

P

M

M

G

D

R

R

V

V

T

T

D

L

P

P

L

L

A

K

L

K

F

M

G

G

T

V

R

S

A

I

Y

S

G

G

R

Q

K

T

G

E

G

R

R

D

L

L

P

P

-

P

M

L

T

R

L

L

V

K

G

G

A

T

A

K

D

N

P

L

V

R

P

P

V

I

R

H

Y

Y

T

E

V

L

P

P

M

I

P

A

S
|
S

A

A

Q
|
Q

V

V

K

K

S

S

A

A

V

L

L

M

L

F

A

A

G

A

L

L

N

Q

A

A

P

K

G

G

Q

E

T

S

V

V

V

I

I

I

E

E

R

K

E

E

A

Y

T

T

R
|
R

D

N

H

H

S

T

E

E

R

D

M

M

L

L

R

Q

G

Q

F

F

G

G

A

G

E

H

L

L

S

S

V

V

E

-

T

-

G

-

P

-

E

D

G

G

Q

K

I

K

I

I

T

T

L

V

T

Q

G

G

Q

P

P

Q

E

K

L

L

R

T

P

G

Q

Q

T

K

V

V

A

V

V

V

P

P

R

G

D

D

P

I

S

S

A

A

F

F

P

W

V

L

C

V

A

A

A

G

L

L

I

I

V

A

E

P

G

N

S

S

E

R

I

L

L

V

V

L

P

Q

G

N

V

V

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N

N

P

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T

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T

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G

L

I

Y

I

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D

T

V

L

I

L

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A

M

M

G

G

A

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D

K

I

L

A

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F

I

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T

N

E

E

I

R

-

E

-

G

-

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D

P

P

V

V

A

A

D

K

-

S

-

A

-

T

L

L

R

I

V

V

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E

A

S

S

S

A

D

L

L

K

K

G

G

V

T

E

E

V

I

P

C

P

G

E

A

R

L

A

I

P

P

S

R

M

L

I
|
I

D
|
D

E

E

Y

L

P

P

I

I

L

I

S

A

V

L

A

A

A

T

F

Q

A

A

E

Q

G

G

S

V

T

T

I

V

M

I

R

K

G

D

V

A

K

E

E

E

L

L

R

K

V

V

K
|
K

E
|
E

S

T

D

D

R
|
R

I

I

D

Q

A

V

M

V

A

A

R

D

G

A

L

L

E

N

A

S

C

M

G

G

V

A

R

D

I

I

E

T

E

P

D

T

E

A

D

D

T

G

L

M

I

I

V

I

H

K

G

G

M

K

G

S

R

A

V

L

P

H

G

-

G

G

A

A

T

R

C

V

A

N

T

T

H

F

L

G

D

D

H
|
H

R
|
R

I

I

A

G

M

M

S

M

F

T

L

A

V

I

L

A

G

A

-

L

M

L

A

V

A

A

E

D

A

G

P

E

V

V

T

E

V

L

D

D

D

R

G

A

S

E

P

A

I

I

A

N

T
|
T

S

S

F

Y

P

P

A

S

F

F

T

F

D

D

L

D

M

L

T

E

G

S

L

L

active site: K20 (= K28), S21 (= S29), D47 (= D55), N90 (= N99), D115 (≠ A124), R120 (= R129), D312 (= D326), E340 (= E354), H384 (= H399), R385 (= R400), T412 (= T426)
binding glyphosate: K20 (= K28), G92 (= G101), T93 (= T102), R120 (= R129), Q168 (= Q176), D312 (= D326), E340 (= E354), R343 (= R357), H384 (= H399), R385 (= R400)
binding shikimate-3-phosphate: S21 (= S29), R25 (= R33), S166 (= S174), Q168 (= Q176), R193 (= R201), I311 (= I325), D312 (= D326), K339 (= K353)

1rf4A Structural studies of streptococcus pneumoniae epsp synthase, tetrahedral intermediate bound state (see paper)
40% identity, 95% coverage: 18:438/445 of query aligns to 10:424/427 of 1rf4A

query
sites
1rf4A

L

L

K

H

G

G

R

I

A

I

E

R

I

V

P

P

G

G

D

D

K
|
K

S
|
S

I

I

S

S

H

H

R
|
R

A

S

L

I

I

I

L

F

G

G

A

S

M

L

A

A

V

E

G

G

E

E

T

T

Q

K

I

V

T

Y

G

D

L

I

L

L

E

R

G

G