SitesBLAST
Comparing WP_011869215.1 NCBI__GCF_000016125.1:WP_011869215.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5i92F Crystal structure of glutamate-1-semialdehyde 2,1- aminomutase (gsa) from pseudomonas aeruginosa
49% identity, 98% coverage: 7:423/427 of query aligns to 1:417/420 of 5i92F
Sites not aligning to the query:
3bs8A Crystal structure of glutamate 1-semialdehyde aminotransferase complexed with pyridoxamine-5'-phosphate from bacillus subtilis (see paper)
49% identity, 97% coverage: 8:421/427 of query aligns to 5:421/430 of 3bs8A
- active site: V22 (= V25), Y145 (≠ F145), E207 (= E207), D240 (= D240), M243 (≠ I243), K268 (= K268), G401 (≠ C401)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G118 (= G118), T119 (= T119), Y145 (≠ F145), H146 (= H146), E207 (= E207), N212 (= N212), D240 (= D240), V242 (= V242), K268 (= K268)
3k28A Crystal structure of a glutamate-1-semialdehyde aminotransferase from bacillus anthracis with bound pyridoxal 5'phosphate
49% identity, 99% coverage: 6:427/427 of query aligns to 3:422/423 of 3k28A
- active site: V22 (= V25), Y145 (≠ F145), E202 (= E207), D235 (= D240), M238 (≠ I243), K263 (= K268), G396 (≠ C401)
- binding calcium ion: I103 (= I103), V106 (≠ I106), P107 (= P107), I109 (≠ A109)
- binding pyridoxal-5'-phosphate: G118 (= G118), T119 (= T119), Y145 (≠ F145), H146 (= H146), G147 (= G147), E202 (= E207), D235 (= D240), V237 (= V242), M238 (≠ I243), K263 (= K268)
Q42522 Glutamate-1-semialdehyde 2,1-aminomutase 2, chloroplastic; GSA 2; Glutamate-1-semialdehyde aminotransferase 2; GSA-AT 2; EC 5.4.3.8 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
49% identity, 99% coverage: 7:427/427 of query aligns to 48:472/472 of Q42522
- R92 (≠ Y48) mutation to K: In gsa2-1; suppression of enf1 mutant pleiotropic developmental phenotypes; when associated with S-162.
- G162 (= G118) mutation to S: In gsa2-1; suppression of enf1 mutant pleiotropic developmental phenotypes; when associated with K-92.
3fqaA Gabaculien complex of gabaculine resistant gsam version (see paper)
48% identity, 99% coverage: 6:427/427 of query aligns to 1:426/426 of 3fqaA
- active site: V20 (= V25), Y143 (≠ F145), D238 (= D240), I241 (= I243), K266 (= K268), A400 (≠ C401)
- binding 3-aminobenzoic acid: S22 (= S27), R25 (= R30), W60 (≠ Y62)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G116 (= G118), T117 (= T119), Y143 (≠ F145), E205 (= E207), N210 (= N212), D238 (= D240), V240 (= V242), I241 (= I243)
2gsaB Crystal structure of glutamate-1-semialdehyde aminomutase (aminotransferase, wild-type form) (see paper)
47% identity, 99% coverage: 5:427/427 of query aligns to 1:427/427 of 2gsaB
- active site: V21 (= V25), Y144 (≠ F145), E206 (= E207), D239 (= D240), M242 (≠ I243), K267 (= K268), A401 (≠ C401)
- binding pyridoxal-5'-phosphate: G117 (= G118), T118 (= T119), Y144 (≠ F145), E206 (= E207), N211 (= N212), D239 (= D240), V241 (= V242), M242 (≠ I243), K267 (= K268)
2gsaA Crystal structure of glutamate-1-semialdehyde aminomutase (aminotransferase, wild-type form) (see paper)
47% identity, 99% coverage: 5:427/427 of query aligns to 1:427/427 of 2gsaA
- active site: V21 (= V25), Y144 (≠ F145), E206 (= E207), D239 (= D240), M242 (≠ I243), K267 (= K268), A401 (≠ C401)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G118), T118 (= T119), Y144 (≠ F145), H145 (= H146), G146 (= G147), N211 (= N212), D239 (= D240), V241 (= V242), K267 (= K268)
3usfA Crystal structure of dava-4
47% identity, 99% coverage: 5:427/427 of query aligns to 1:427/427 of 3usfA
- active site: V21 (= V25), Y144 (≠ F145), D239 (= D240), M242 (≠ I243), K267 (= K268), A401 (≠ C401)
- binding (4s)-4,5-diaminopentanoic acid: S23 (= S27), V25 (= V29), S157 (= S158), K267 (= K268), E400 (= E400)
- binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: G117 (= G118), T118 (= T119), Y144 (≠ F145), N211 (= N212), D239 (= D240), V241 (= V242), K267 (= K268)
3fq7A Gabaculine complex of gsam (see paper)
47% identity, 99% coverage: 5:427/427 of query aligns to 1:427/427 of 3fq7A
- active site: V21 (= V25), Y144 (≠ F145), D239 (= D240), M242 (≠ I243), K267 (= K268), A401 (≠ C401)
- binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: S23 (= S27), V25 (= V29), W61 (≠ Y62), G117 (= G118), T118 (= T119), Y144 (≠ F145), H145 (= H146), E206 (= E207), N211 (= N212), D239 (= D240), V241 (= V242), M242 (≠ I243), K267 (= K268), G298 (= G299), T299 (= T300), E400 (= E400)
2hp2A Inter-subunit signaling in gsam (see paper)
47% identity, 99% coverage: 5:427/427 of query aligns to 1:427/427 of 2hp2A
- active site: V21 (= V25), Y144 (≠ F145), D239 (= D240), M242 (≠ I243), K267 (= K268), A401 (≠ C401)
- binding (4s)-4,5-diaminopentanoic acid: G298 (= G299), T299 (= T300)
- binding (4r)-5-amino-4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]pentanoic acid: S23 (= S27), G117 (= G118), T118 (= T119), Y144 (≠ F145), H145 (= H146), E206 (= E207), N211 (= N212), D239 (= D240), V241 (= V242), M242 (≠ I243), K267 (= K268)
- binding pyridoxal-5'-phosphate: G298 (= G299), T299 (= T300)
2hp1A Inter-subunit signaling in gsam (see paper)
47% identity, 99% coverage: 5:427/427 of query aligns to 1:427/427 of 2hp1A
- active site: V21 (= V25), Y144 (≠ F145), D239 (= D240), M242 (≠ I243), K267 (= K268), A401 (≠ C401)
- binding (4s)-4-amino-5-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]pentanoic acid: S23 (= S27), V25 (= V29), W61 (≠ Y62), S116 (= S117), G117 (= G118), T118 (= T119), Y144 (≠ F145), H145 (= H146), G146 (= G147), E206 (= E207), N211 (= N212), D239 (= D240), V241 (= V242), M242 (≠ I243), K267 (= K268), E400 (= E400)
2hozA Inter-subunit signaling in gsam (see paper)
47% identity, 99% coverage: 5:427/427 of query aligns to 1:427/427 of 2hozA
- active site: V21 (= V25), Y144 (≠ F145), D239 (= D240), M242 (≠ I243), K267 (= K268), A401 (≠ C401)
- binding (4s)-4,5-diaminopentanoic acid: E141 (= E142), G156 (= G157), S157 (= S158), P182 (= P183), N368 (≠ T368), E370 (= E370), K373 (= K373)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G118), T118 (= T119), Y144 (≠ F145), H145 (= H146), G146 (= G147), E206 (= E207), N211 (= N212), D239 (= D240), G298 (= G299), T299 (= T300)
P42799 Glutamate-1-semialdehyde 2,1-aminomutase 1, chloroplastic; AtGSA1; GSA 1; Glutamate-1-semialdehyde aminotransferase 1; GSA-AT 1; EC 5.4.3.8 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
47% identity, 99% coverage: 4:427/427 of query aligns to 47:474/474 of P42799
- K314 (= K268) modified: N6-(pyridoxal phosphate)lysine
5hdmB Crystal structure of arabidopsis thaliana glutamate-1-semialdehyde-2, 1-aminomutase (see paper)
47% identity, 99% coverage: 4:427/427 of query aligns to 1:428/428 of 5hdmB
- active site: V22 (= V25), Y145 (≠ F145), E207 (= E207), D240 (= D240), M243 (≠ I243), K268 (= K268), A402 (≠ C401)
- binding pyridoxal-5'-phosphate: G118 (= G118), T119 (= T119), Y145 (≠ F145), E207 (= E207), N212 (= N212), D240 (= D240), V242 (= V242), M243 (≠ I243), K268 (= K268)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G118 (= G118), T119 (= T119), Y145 (≠ F145), E207 (= E207), N212 (= N212), D240 (= D240), V242 (= V242), M243 (≠ I243), K268 (= K268)
5hdmA Crystal structure of arabidopsis thaliana glutamate-1-semialdehyde-2, 1-aminomutase (see paper)
47% identity, 99% coverage: 4:427/427 of query aligns to 1:428/428 of 5hdmA
- active site: V22 (= V25), Y145 (≠ F145), E207 (= E207), D240 (= D240), M243 (≠ I243), K268 (= K268), A402 (≠ C401)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G118 (= G118), T119 (= T119), Y145 (≠ F145), G147 (= G147), E207 (= E207), N212 (= N212), D240 (= D240), V242 (= V242), K268 (= K268)
6w80A Crystal structure of glutamate-1-semialdehyde 2,1-aminomutase from stenotrophomonas maltophilia k279a in complex with plp
46% identity, 97% coverage: 4:417/427 of query aligns to 5:419/430 of 6w80A
- active site: V26 (= V25), Y149 (≠ F145), D241 (= D240), K269 (= K268)
- binding pyridoxal-5'-phosphate: S121 (= S117), G122 (= G118), T123 (= T119), Y149 (≠ F145), H150 (= H146), E208 (= E207), N213 (= N212), D241 (= D240), V243 (= V242), K269 (= K268)
P23893 Glutamate-1-semialdehyde 2,1-aminomutase; GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT; EC 5.4.3.8 from Escherichia coli (strain K12) (see paper)
46% identity, 99% coverage: 7:427/427 of query aligns to 1:426/426 of P23893
- K265 (= K268) mutation to R: 2% of wild-type activity.
2e7uA Crystal structure of glutamate-1-semialdehyde 2,1-aminomutase from thermus thermophilus hb8
45% identity, 98% coverage: 7:424/427 of query aligns to 1:424/424 of 2e7uA
- active site: V21 (= V25), Y144 (≠ F145), E206 (= E207), D238 (= D240), M241 (≠ I243), K266 (= K268), A401 (≠ C401)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G118), T118 (= T119), Y144 (≠ F145), H145 (= H146), N211 (= N212), D238 (= D240), V240 (= V242)
2zsmA Crystal structure of glutamate-1-semialdehyde 2,1-aminomutase from aeropyrum pernix, hexagonal form
42% identity, 98% coverage: 8:427/427 of query aligns to 7:422/425 of 2zsmA
- active site: V24 (= V25), Y145 (≠ F145), E201 (= E207), D234 (= D240), V237 (≠ I243), K262 (= K268), A396 (≠ C401)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S117 (= S117), G118 (= G118), T119 (= T119), Y145 (≠ F145), H146 (= H146), E201 (= E207), D234 (= D240), K262 (= K268)
3usfB Crystal structure of dava-4
44% identity, 99% coverage: 5:427/427 of query aligns to 1:402/402 of 3usfB
- active site: V21 (= V25), Y144 (≠ F145), E181 (= E207), D214 (= D240), M217 (≠ I243), K242 (= K268), A376 (≠ C401)
- binding (4s)-4,5-diaminopentanoic acid: G273 (= G299), T274 (= T300)
- binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: G117 (= G118), T118 (= T119), Y144 (≠ F145), E181 (= E207), N186 (= N212), D214 (= D240), V216 (= V242), M217 (≠ I243), K242 (= K268)
Query Sequence
>WP_011869215.1 NCBI__GCF_000016125.1:WP_011869215.1
MELNLKMDRSKELFEESKKYLVGGVNSPVRSFKPFPFFVKSAKDCFLYDEDGNEFIDYCL
AYGPMVLGHANENILNAVKSQMDLGTAYGVPSEKEITLAKEVINRIPCAEMVRFVNSGTE
ATMGAIRLARGVTGRDKIIKFEGAFHGAHDYVLVKTGSGALTHGAPNSPGIPEDTTKNTL
LIPFNDEDAVRKVISENKDEIACIILEPVMGNVGCILPQDGYLQFLREITEENGILLIFD
EVITGFRLSKGGAQEYFGIKSDLATIGKILGGGFPIGAITGKKEYMEQFSPSGQIYQAGT
FNGNPVSVTAGIETLKNLDDKFYKETTKKADILSNFLRETAEKYNVSTKVYNVASIFQIY
FNEKEVVTYEDAKSSDTEKFMRYFYTLLENGVFVAPSQFECCFTSIKHNDEVLEKTMNAI
DIAMKKL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory