SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011869448.1 NCBI__GCF_000016125.1:WP_011869448.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

F9VNG5 Homoserine dehydrogenase; HDH; HSD; StHSD; EC 1.1.1.3 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 3 papers)
36% identity, 98% coverage: 1:329/337 of query aligns to 1:297/304 of F9VNG5

query
sites
F9VNG5

M

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Y8 (≠ F8) binding NADP(+)
N10 (≠ I10) binding NADP(+)
V11 (≠ I11) binding NADP(+)
R38 (= R42) binding NADP(+)
R39 (≠ S43) binding NADP(+)
S73 (= S95) binding NADP(+)
T100 (≠ A122) binding NADP(+)
K102 (= K124) binding NADP(+)
G182 (= G206) binding NADP(+)
E185 (= E209) binding NADP(+)
G284 (= G316) binding NADP(+)

Sites not aligning to the query:

303 modified: Interchain

Q5F8J4 Homoserine dehydrogenase; HDH; HSD; NAD(+)-dependent homoserine dehydrogenase; NAD(+)-dependent HSD; NgHSD; EC 1.1.1.3 from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) (see paper)
36% identity, 92% coverage: 23:331/337 of query aligns to 26:316/435 of Q5F8J4

query
sites
Q5F8J4

S

A

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L45 (≠ R42) mutation to R: Shows a marked increase in the catalytic efficiency with NADP(+).
LS 45:46 (≠ RS 42:43) mutation to RD: Does not impair the catalytic activity with NAD(+). Slightly increases the activity, but slightly decreases the affinity for NADP(+).; mutation to RR: Causes a shift in coenzyme preference from NAD(+) to NADP(+) by a factor of 974. Shows a slight decrease in the catalytic efficiency with NAD(+) and a 4.5-fold increase in catalytic efficiency with NADP(+).

5x9dA Crystal structure of homoserine dehydrogenase in complex with l- cysteine and NAD (see paper)
35% identity, 98% coverage: 1:329/337 of query aligns to 1:295/302 of 5x9dA

query
sites
5x9dA

M

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active site: D196 (= D220), K200 (= K224)
binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: F6 (≠ V6), G7 (= G7), Y8 (≠ F8), G9 (= G9), N10 (≠ I10), V11 (≠ I11), T37 (≠ D41), R38 (= R42), V72 (= V94), S73 (= S95), S74 (≠ P96), P82 (= P104), T100 (≠ A122), N101 (= N123), K102 (= K124), G127 (≠ A149), S131 (≠ G153), N155 (= N178), G156 (= G179), T157 (= T180), Y183 (≠ I207), A184 (= A208), E185 (= E209), D191 (= D215), D196 (= D220), K200 (= K224), A281 (= A315), G282 (= G316), A286 (≠ T320)

7f4cA The crystal structure of the immature holo-enzyme of homoserine dehydrogenase complexed with NADP and 1,4-butandiol from the hyperthermophilic archaeon sulfurisphaera tokodaii. (see paper)
36% identity, 98% coverage: 1:329/337 of query aligns to 1:293/300 of 7f4cA

query
sites
7f4cA

M

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I

L

L

T

T

K

L

M

M

E

N

K

K

E

-

Q

G

L

V

D

S

F

F

D

E

T

E

V

A

L

L

K

K

E

E

A

A

Q

Q

E

R

L

R

G

G

I

Y

A

A

E
|
E

T

E

N

D

P

P

H

T

Q

L

D

D

I

I

S

N

G

G

L

F

D

D

T

A

A

A

K

K

I

I

V

T

I

I

L

L

A

A

N

N

S

F

I

M

F

I

G

G

R

N

D

S

V

V

T

T

I

I

K

K

D

D

V

V

N

K

L

F

E

E

G

G

I

I

T

N

R

R

I

-

T

-

P

-

E

-

A

-

L

-

A

-

M

-

A

-

N

-

K

-

S

-

G

D

H

L

T

K

I

I

K

K

L

L

I

I

G

A

E

Y

V

A

T

D

D

E

K

K

K

E

L

V

E

W

V

V

C

K

P

P

K

L

L

P

I

I

P

S

I

Q

D

D

S

D

P

P

L

L

-

Y

N

N

V

V

K

D

G

G

S

V

L

E

N

N

V

A

A

L

M

E

V

I

N

T

T

T

D

D

L

I

A

Q

N

S

D

-

I

I

V

L

V

I

V

R

G

G

A

P

G

G

A

A

G
|
G

D

P

I

V

E

N

T
x
A

A

A

S

Y

A

G

I

A

L

L

S

S

D

D

L

L

V

I

binding nadp nicotinamide-adenine-dinucleotide phosphate: F6 (≠ V6), G7 (= G7), Y8 (≠ F8), G9 (= G9), N10 (≠ I10), V11 (≠ I11), T37 (≠ D41), R38 (= R42), R39 (≠ S43), V72 (= V94), S73 (= S95), S74 (≠ P96), T100 (≠ A122), K102 (= K124), G127 (≠ A149), S131 (≠ G153), E185 (= E209), G280 (= G316), A284 (≠ T320)

6dzsA Mycobacterial homoserine dehydrogenase thra in complex with NADP
35% identity, 97% coverage: 3:329/337 of query aligns to 7:319/431 of 6dzsA

query
sites
6dzsA

I

V

I

A

L

V

V

L

G
|
G

F
x
L

G
|
G

I
x
N

I
x
V

G

G

K

S

G

E

V

V

L

V

K

R

T

I

I

I

T

D

L

E

K

S

S

A

E

T

H

D

L

L

K

A

R

R

Y

I

G

G

M

A

D

P

L

L

Q

Q

V

L

A

R

A

G

I

I

C

G

D
x
V

R
|
R

S
x
R

G

V

A

S

A

A

I

-

D

-

E

D

N
x
R

G

G

L

V

D

P

L

V

E

E

L

L

A

L

L

T

K

D

I

-

K

-

E

-

T

-

G

-

K

-

I

-

A

-

N

-

Y

-

P

-

E

-

K

-

G

-

C

-

E

-

M

-

G

N

I
|
I

L

E

E

E

V

L

I

V

E

S

S

R

V

D

S

D

A

V

D

D

A

I

V

V

E

E

V
x
L

-
x
M

S

G

P

P

T

V

N

-

I

-

E

-

T

-

G

-

E

E

P
|
P

A

A

K

R

S

K

Y

A

M

I

L

L

K

T

A

A

F

L

E

E

C

Q

K

G

K

K

H

S

V

V

S

T

A
|
A

N

N

K
|
K

G

A

P

L

L

M

A

S

V

V

S

S

F

T

K

G

D

E

L

L

V

A

K

Q

C

A

A

A

K

E

E

A

N

A

K

H

V

V

C

D

F

L

R

Y

Y

F

E

E

A

A

S

A

V

V

G

A

G

G

A

A

M

I

P

P

I

V

I

I

N

R

L

P

A

L

K

T

E

Q

T

S

L

L

A

A

G

G

N

D

D

T

I

V

K

T

L

R

I

V

K

A

G

G

I

I

L

V

N

N

G

G

T

T

N

N

Y

Y

I

I

L

L

T

S

K

A

M

M

E

D

K

S

E

T

Q

G

L

A

D

D

F

Y

D

G

T

D

V

A

L

L

K

A

E

E

A

A

Q

S

E

A

L

L

G

G

I

Y

A

A

E

E

T

A

N

D

P

P

H

T

Q

A

D

D

I

V

S

E

G

G

L

Y

D

D

T

A

A

A

K

K

I

A

V

A

I

I

L

L

A

A

N

S

S

I

I

A

F

F

G

H

R

T

D

R

V

V

T

T

I

A

K

D

D

D

V

V

N

Y

L

R

E

E

G

G

I

I

T

T

R

K

I

V

T

T

P

A

E

A

A

D

L

F

A

A

M

S

A

A

N

R

K

A

S

L

G

G

H

C

T

T

I

I

K

K

L

L

I

L

G

A

-

I

-

C

-

E

E

R

V

L

T

T

D

S

K

D

-

D

-

G

-

H

-

Q

-

S

-

V

K

S

L

A

E

R

V

V

C

Y

P

P

K

A

L

L

I

V

P

P

I

L

D

T

S

H

P

P

L

L

-

A

N

A

V

V

K

N

G

G

S

A

L

F

N

N

V

A

A

V

M

V

V

V

N

E

T

A

D

E

L

A

A

A

N

G

D

R

I

L

V

M

V

F

V

Y

G

G

A

Q

G

G

A

A

G
|
G

D

G

I

A

E

P

T
|
T

A

A

S

S

A

A

I

V

L

M

S

G

D

D

L

V

V

V

binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G7), L12 (≠ F8), G13 (= G9), N14 (≠ I10), V15 (≠ I11), V45 (≠ D41), R46 (= R42), R47 (≠ S43), R52 (≠ N50), I63 (= I79), L78 (≠ V94), M79 (vs. gap), P84 (= P104), A102 (= A122), K104 (= K124), G306 (= G316), T310 (= T320)

4pg7A Crystal structure of s. Aureus homoserine dehydrogenase at ph7.5 (see paper)
34% identity, 98% coverage: 1:331/337 of query aligns to 5:306/402 of 4pg7A

query
sites
4pg7A

M

L

K

N

I

I

I

A

L

L

V

L

G

G

F

L

G

G

I

T

I

V

G

G

K

S

G

G

V

V

L

V

K

K

T

I

I

I

T

E

L

E

K

N

S

R

E

Q

H

Q

L

I

K

Q

K

D

R

T

Y

L

G

N

M

K

D

D

L

I

Q

V

V

I

A

K

A

H

I

I

C

L

D

V

R

R

S

D

G

-

A

-

I

-

D

-

E

-

N

-

G

-

L

-

D

-

L

-

E

-

L

-

A

-

L

-

K

K

I

S

K

K

E

K

E

R

T

P

G

L

K

N

I

I

A

S

N

Q

Y

Y

P

H

E

-

K

-

G

-

C

L

E

T

M

E

G

D

I

V

L

N

E

E

V

I

I

L

E

N

S

D

V

D

S

S

A

L

D

D

A

I

V

I

V

V

E

E

V

V

S

-

P

-

T

-

N

-

I

M

E

G

T

G

G

I

E

E

P

P

A

T

K

V

S

D

Y

W

M

L

L

R

K

T

A

A

F

L

E

K

C

N

K

K

H

H

V

V

V

I

S

T

A

A

N

N

K

K

G

D

P

L

L

L

A

A

V

V

S

H

F

L

K

K

D

L

L

L

V

E

K

D

C

L

A

A

K

E

E

E

N

N

K

G

V

V

C

A

F

L

R

K

Y

F

E

E

A

A

S

S

V

V

G

A

G

G

A

G

M

-

P

-

I

-

N

-

L

-

A

-

K

-

E

-

T

-

L

-

A

-

G

P

N

N

D

N

I

I

K

S

L

K

I

F

K

M

G

G

I

I

L

L

N

N

G

G

T

T

T

S

N

N

Y

F

I

I

L

L

T

S

K

K

M

M

E

T

K

K

E

E

Q

Q

L

T

D

T

F

F

D

E

T

E

V

A

L

L

K

D

E

E

A

A

Q

K

E

R

L

L

G

G

I

F

A

A

E

E

T

A

N

D

P

P

H

T

Q

D

D

D

I

V

S

E

G

G

L

V

D
|
D

T

A

A

A

A

R

K
|
K

I

V

V

V

I

I

L

T

A

S

N

Y

S

L

I

S

F

F

G

N

R

Q

D

V

V

I

T

K

I

L

K

N

D

D

V

V

N

K

L

R

E
x
R

G

G

I

I

T

S

R
x
G

I

V

T

T

P

L

E

T

A

D

L

I

A

N

M

V

A

A

N

D

K

Q

S

L

G

G

H

Y

T

K

I

I

K

K

L

L

I

I

G

G

-

K

-

G

-

I

-

Y

E

E

V

N

T

G

D

K

K

V

K

N

L

A

E

S

V

V

C

E

P

P

K

T

L

L

I

I

P

D

I

K

D

K

S

H

P

Q

L

L

-

A

N

A

V

V

K

E

G

D

S

E

L

Y

N

N

V

A

A

I

M

Y

V

V

N

I

T

G

D

D

L

A

A

V

N

G

D

D

I

T

V

M

V

F

V

Y

G

G

A

K

G

G

A

A

G

G

D

S

I

L

E

A

T

T

A

G

S

S

A

A

I

V

L

V

S

S

D

D

L

L

V

L

N

N

I

V

active site: D190 (= D220), K194 (= K224)
binding lysine: R215 (≠ E245), G219 (≠ R249)

Sites not aligning to the query:

binding lysine: 394, 395

4xb1A Hyperthermophilic archaeal homoserine dehydrogenase in complex with NADPH (see paper)
31% identity, 100% coverage: 1:336/337 of query aligns to 3:314/319 of 4xb1A

query
sites
4xb1A

M

V

K

N

I

I

I

S

L

I

V
x
F

G

G

F
|
F

G
|
G

I
x
T

I
x
V

G

G

K

R

G

A

V

L

L

A

K

E

T

I

I

I

T

A

L

E

K

K

S

S

E

-

H

-

L

-

K

-

K

R

R

I

Y

F

G

G

M

V

D

E

L

L

Q

N

V

V

A

I

A

S

I

I

C

T

D
|
D

R
|
R

S

S

G

G

A

T

A

I

I

W

D

G

E

D

N

-

G

-

L

F

D

D

L

L

E

L

L

E

A

A

L

K

K

E

I

V

K
|
K

E

E

E

S

T

T

G

G

K

K

I

L

A

S

N

N

Y

-

P

-

E

-

K

-

G

-

C

-

E

-

M

I

G

G

I

D

L

Y

E

E

V

V

I

Y

E

N

S

F

V

S

S

P

A

Q

D

E

A

L

V

V

V

E

E

E

V

V

S

K

P

P

T

N

-

I

-

L

-

V

N

D

I
x
V

E
x
S

T
x
S

G

W

E

D

P

E

A

A

K

H

S

E

Y

M

M

Y

L

K

K

V

A

A

F

L

E

G

C

E

K

G

K

I

H

S

V

V

S

T

A
x
S

N

N

K
|
K

G

P

P

P

L

I

A

A

V

N

S

Y

F

Y

K

D

D

E

L

L

V

M

K

N

C

L

A

A

K

K

E

E

N

N

K

N

V

A

C

G

F

I

R

F

Y

F

E

E

A
x
S

S

T

V

V

G

M

G

A

A

G

M

T

P

P

I

I

N

G

L

V

A

L

K

R

E

E

T

N

L

L

A

L

G

G

N

E

D

N

I

I

K

K

L

R

I

I

K

D

G

A

I

V

L

V

N

N

G

A

T

S

T

T

N

T

Y

F

I

I

L

L

T

T

K

K

M

M

E

-

K

S

E

E

Q

G

L

K

D

T

F

L

D

D

T

D

V

A

L

I

K

E

E

E

A

A

Q

K

E

S

L

L

G

G

I

I

A

L

E

E

T

E

N

D

P

P

H

S

Q

K

D

D

I

I

S

D

G

G

L

I

D
|
D

T

A

A

Y

K
|
K

I

A

V

K

I

I

L

L

A

H

N

W

S

V

I

S

F

Y

G

G

R

E

D

-

V

-

T

P

I

P

K

E

D

E

V

E

N

E

L

R

E

L

G

G

I

I

T

R

R

E

I

V

T

-

P

-

E

-

A

-

L

-

A

-

M

-

A

-

N

-

K

R

S

D

G

A

H

R

T

N

I

V

K

R

L

L

I

V

G

A

E

Q

V

V

T

S

D

K

K

G

K

K

L

I

E

S

V

V

C

K

P

P

K

R

L

K

I

L

P

S

I

S

D

D

S

N

P

P

L

L

N

L

V

V

K

E

G

G

S

V

L

Q

N

N

V

A

A

A

M

V

V

I

N

R

T

T

D

N

L

N

A

L

N

G

D

E

I

V

V

I

V

L

V

K

G

G

A

P

G

G

A
x
G

G

G

D

G

I

R

E

V

T
|
T

A

A

S

S

A

G

I

V

L

F

S

T

D

D

L

I

V

-

N

-

I

I

H

K

Q

A

T

T

L

L

K

K

active site: D211 (= D220), K215 (= K224)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ V6), F10 (= F8), G11 (= G9), T12 (≠ I10), V13 (≠ I11), D39 (= D41), R40 (= R42), K57 (= K61), V91 (≠ I99), S92 (≠ E100), S93 (≠ T101), S114 (≠ A122), K116 (= K124), S141 (≠ A149), G295 (≠ A315), T300 (= T320)

O58802 Homoserine dehydrogenase; HDH; HSD; HseDH; EC 1.1.1.3 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
31% identity, 100% coverage: 1:336/337 of query aligns to 3:314/319 of O58802

query
sites
O58802

M

V

K

N

I

I

I

S

L

I

V

F

G

G

F
|
F

G

G

I
x
T

I
x
V

G

G

K

R

G

A

V

L

L

A

K

E

T

I

I

I

T

A

L

E

K

K

S

S

E

-

H

-

L

-

K

-

K

R

R

I

Y

F

G

G

M

V

D

E

L

L

Q

N

V

V

A

I

A

S

I

I

C

T

D

D

R
|
R

S

S

G

G

A

T

A

I

I

W

D

G

E

D

N

-

G

-

L

F

D

D

L

L

E

L

L

E

A

A

L

K

K

E

I

V

K
|
K

E

E

E

S

T

T

G

G

K

K

I

L

A

S

N

N

Y

-

P

-

E

-

K

-

G

-

C

-

E

-

M

I

G

G

I

D

L

Y

E

E

V

V

I

Y

E

N

S

F

V

S

S

P

A

Q

D

E

A

L

V

V

V

E

E

E

V

V

S

K

P

P

T

N

-

I

-

L

-

V

N

D

I

V

E
x
S

T
x
S

G

W

E

D

P

E

A

A

K

H

S

E

Y

M

M

Y

L

K

K

V

A

A

F

L

E

G

C

E

K

G

K

I

H

S

V

V

S

T

A
x
S

N

N

K
|
K

G

P

P

P

L

I

A

A

V

N

S

Y

F

Y

K

D

D

E

L

L

V

M

K

N

C

L

A

A

K

K

E

E

N

N

K

N

V

A

C

G

F

I

R

F

Y

F

E
|
E

A

S

S

T

V
|
V

G

M

G
x
A

A

G

M
x
T

P

P

I

I

N

G

L

V

A

L

K

R

E

E

T

N

L

L

A

L

G

G

N

E

D

N

I

I

K

K

L

R

I

I

K

D

G

A

I

V

L

V

N

N

G

A

T

S

T

T

N

T

Y

F

I

I

L

L

T

T

K

K

M

M

E

-

K

S

E

E

Q

G

L

K

D

T

F

L

D

D

T

D

V

A

L

I

K

E

E

E

A

A

Q

K

E

S

L

L

G

G

I

I

A

L

E

E

T

E

N

D

P

P

H

S

Q

K

D

D

I

I

S

D

G

G

L

I

D

D

T

A

A

Y

K

K

I

A

V

K

I

I

L

L

A

H

N

W

S

V

I

S

F

Y

G

G

R

E

D

-

V

-

T

P

I

P

K

E

D

E

V

E

N

E

L

R

E

L

G

G

I

I

T

R

R

E

I

V

T

-

P

-

E

-

A

-

L

-

A

-

M

-

A

-

N

-

K

R

S

D

G

A

H

R

T

N

I

V

K

R

L

L

I

V

G

A

E

Q

V

V

T

S

D

K

K

G

K

K

L

I

E

S

V

V

C

K

P

P

K

R

L

K

I

L

P

S

I

S

D

D

S

N

P

P

L

L

N

L

V

V

K

E

G

G

S

V

L

Q

N

N

V

A

A

A

M

V

V

I

N

R

T

T

D

N

L

N

A

L

N

G

D

E

I

V

V

I

V

L

V

K

G

G

A

P

G

G

A

G

G
|
G

D

G

I

R

E

V

T

T

A

A

S

S

A

G

I

V

L

F

S

T

D

D

L

I

V

-

N

-

I

I

H

K

Q

A

T

T

L

L

K

K

F10 (= F8) binding NADPH
T12 (≠ I10) binding NADPH
V13 (≠ I11) binding NADPH
R40 (= R42) binding NADPH; mutation to A: Increases activity with NADP.
K57 (= K61) binding NADPH; mutation to A: Increases activity with NADP.
S92 (≠ E100) binding NADPH
S93 (≠ T101) binding NADPH
S114 (≠ A122) binding NADPH
K116 (= K124) binding NADPH
E140 (= E148) binding Na(+)
V143 (= V151) binding Na(+)
A145 (≠ G153) binding Na(+)
T147 (≠ M155) binding Na(+)
G296 (= G316) binding NADPH

4xb2A Hyperthermophilic archaeal homoserine dehydrogenase mutant in complex with NADPH (see paper)
31% identity, 100% coverage: 1:336/337 of query aligns to 3:314/319 of 4xb2A

query
sites
4xb2A

M

V

K

N

I

I

I

S

L

I

V
x
F

G

G

F
|
F

G
|
G

I
x
T

I
x
V

G

G

K

R

G

A

V

L

L

A

K

E

T

I

I

I

T

A

L

E

K

K

S

S

E

-

H

-

L

-

K

-

K

R

R

I

Y

F

G

G

M

V

D

E

L

L

Q

N

V

V

A

I

A

S

I

I

C

T

D

D

R
|
R

S

S

G

G

A

T

A

I

I

W

D

G

E

D

N

-

G

-

L

F

D

D

L

L

E

L

L

E

A

A

L

K

K

E

I

V

K

A

E

E

E

S

T

T

G

G

K

K

I

L

A

S

N

N

Y

-

P

-

E

-

K

-

G

-

C

-

E

-

M

I

G

G

I

D

L

Y

E

E

V

V

I

Y

E

N

S

F

V

S

S

P

A

Q

D

E

A

L

V

V

V

E

E

E

V

V

S

K

P

P

T

N

-

I

-

L

-

V

N

D

I
x
V

E
x
S

T
x
S

G

W

E

D

P

E

A

A

K

H

S

E

Y

M

M

Y

L

K

K

V

A

A

F

L

E

G

C

E

K

G

K

I

H

S

V

V

S

T

A
x
S

N
|
N

K
|
K

G

P

P

P

L

I

A

A

V

N

S

Y

F

Y

K

D

D

E

L

L

V

M

K

N

C

L

A

A

K

K

E

E

N

N

K

N

V

A

C

G

F

I

R

F

Y

F

E

E

A
x
S

S

T

V

V

G

M

G

A

A

G

M

T

P

P

I

I

N

G

L

V

A

L

K

R

E

E

T

N

L

L

A

L

G

G

N

E

D

N

I

I

K

K

L

R

I

I

K

D

G

A

I

V

L

V

N

N

G
x
A

T
x
S

T

T

N

T

Y

F

I

I

L

L

T

T

K

K

M

M

E

-

K

S

E

E

Q

G

L

K

D

T

F

L

D

D

T

D

V

A

L

I

K

E

E

E

A

A

Q

K

E

S

L

L

G

G

I

I

A

L

E

E

T

E

N

D

P

P

H

S

Q

K

D
|
D

I

I

S

D

G

G

L

I

D
|
D

T

A

A

Y

K
|
K

I

A

V

K

I

I

L

L

A

H

N

W

S

V

I

S

F

Y

G

G

R

E

D

-

V

-

T

P

I

P

K

E

D

E

V

E

N

E

L

R

E

L

G

G

I

I

T

R

R

E

I

V

T

-

P

-

E

-

A

-

L

-

A

-

M

-

A

-

N

-

K

R

S

D

G

A

H

R

T

N

I

V

K

R

L

L

I

V

G

A

E

Q

V

V

T

S

D

K

K

G

K

K

L

I

E

S

V

V

C

K

P

P

K

R

L

K

I

L

P

S

I

S

D

D

S

N

P

P

L

L

N

L

V

V

K

E

G

G

S

V

L

Q

N

N

V

A

A

A

M

V

V

I

N

R

T

T

D

N

L

N

A

L

N

G

D

E

I

V

V

I

V

L

V

K

G

G

A

P

G

G

A
x
G

G

G

D

G

I

R

E

V

T
|
T

A

A

S

S

A

G

I

V

L

F

S

T

D

D

L

I

V

-

N

-

I

I

H

K

Q

A

T

T

L

L

K

K

active site: D211 (= D220), K215 (= K224)
binding l-homoserine: A171 (≠ G179), S172 (≠ T180), D206 (= D215), K215 (= K224)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ V6), F10 (= F8), G11 (= G9), T12 (≠ I10), V13 (≠ I11), R40 (= R42), V91 (≠ I99), S92 (≠ E100), S93 (≠ T101), S114 (≠ A122), N115 (= N123), K116 (= K124), S141 (≠ A149), G295 (≠ A315), T300 (= T320)

3ingA Crystal structure of homoserine dehydrogenase (np_394635.1) from thermoplasma acidophilum at 1.95 a resolution
33% identity, 73% coverage: 1:247/337 of query aligns to 3:236/319 of 3ingA

query
sites
3ingA

M

I

K

R

I

I

L

L

V

M

G
|
G

F
x
T

G
|
G

I
x
N

I
x
V

G

G

K

L

G

N

V

V

L

L

K

R

T

I

I

I

T

D

L

A

K

S

S

N

E

-

H

-

L

-

K

R

R

R

Y

F

G

S

M

-

D

-

L

I

Q

K

V

V

A

V

A

G

I

V

C

S

D
|
D

R
x
S

S

R

G

S

A

Y

A

A

I

S

D

G

E

R

N

N

G

-

L

L

D

D

L

I

E

S

L

S

A

I

L

I

K

S

I

N

K
|
K

E

E

E

K

T

T

G

G

K

R

I

I

A

S

N

D

Y

R

P

A

E

F

K

S

G

G

C

P

E

E

M

D

G

L

I

M

L

G

E

E

V

-

I

-

E

-

S

-

V

-

S

A

A

A

D

D

A

L

V

L

V

V

E

D

V
x
C

S
x
T

P
|
P

T

A

N

S

I

-

E

R

T

D

G

G

E

V

P

R

A

E

K

Y

S

S

Y

L

M

Y

L

R

K

M

A

A

F

F

E

E

C

S

K

G

K

M

H

N

V

V

S

T

A
|
A

N
|
N

K
|
K

G

S

P

G

L

L

A

A

V

N

S

K

F

W

K

H

D

D

L

I

V

M

K

D

C

S

A

A

K

N

E

Q

N

N

K

S

V

K

C

Y

F

I

R

R

Y

Y

E

E

A
|
A

S

T

V

V

G

A

G

G

A

G

M

V

P

P

I

L

I

F

N

S

L

V

A

L

K

D

E

Y

T

S

L

I

A

L

G

P

N

S

D

K

I

V

K

K

L

R

I

F

K

R

G

G

I

I

L

V

N

S

G

S

T

T

T

I

N

N

Y

Y

I

V

L

I

T

R

K

N

M

M

E

A

K

N

E

G

Q

R

L

-

D

S

F

L

D

R

T

D

V

V

L

V

K

D

E

D

A

A

Q

I

E

K

L

K

G

G

I

I

A

A

E
|
E

T
x
S

N

N

P

P

H

Q

Q

D

D

D

I

L

S

N

G

G

L

L

D
|
D

T

A

A

A

A

R

K
|
K

I

S

V

V

I

I

L

L

A

V

N

N

S

H

I

I

F

F

G

G

R

T

D

E

V

Y

T

T

I

L

K

N

D

D

V

V

N

E

L

Y

E

S

G

G

I

V

active site: D209 (= D220), K213 (= K224)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G7), T10 (≠ F8), G11 (= G9), N12 (≠ I10), V13 (≠ I11), D38 (= D41), S39 (≠ R42), K57 (= K61), C85 (≠ V94), T86 (≠ S95), P87 (= P96), A112 (= A122), N113 (= N123), K114 (= K124), A139 (= A149), E198 (= E209), S199 (≠ T210)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 302, 305

3jsaA Homoserine dehydrogenase from thermoplasma volcanium complexed with NAD
30% identity, 98% coverage: 1:329/337 of query aligns to 1:317/321 of 3jsaA

query
sites
3jsaA

M

M

K

K

I

T

I

I

-

N

-

L

-

S

L

I

V

F

G
|
G

F
x
L

G
|
G

I
x
N

I
x
V

G

G

K

L

G

N

V

L

L

L

K

R

T

I

I

I

T

-

L

-

K

R

S

S

E

F

H

N

L

E

K

E

K

N

R

R

Y

L

G

G

M

L

D

K

L

F

Q

N

V

V

A

V

A

F

I

V

C

A

D
|
D

R
x
S

S

L

G

H

A

S

A

Y

I

Y

D

N

E

E

N

-

G

-

L

-

D

-

L

-

E

-

L

-

A

-

L

-

K

-

I

-

K

R

E

I

E

D

T

I

G

G

K

K

I

V

A

I

N

S

Y

Y

P

K

E

E

K

K

G

G

C

S

E

-

M

L

G

D

I

S

L

L

E

E

V

Y

I

-

E

E

S

S

V

I

S

S

A

A

-

S

-

E

-

A

-

L

-

A

-

R

-

D

-

F

D

D

A

I

V

V

E

D

V
x
A

S
x
T

P
|
P

T

A

N

S

I

A

E

D

T

-

G

G

E

K

P

K

A

E

K

L

S

A

Y

F

M

Y

L

K

K

E

A

T

F

F

E

E

C

N

K

G

K

K

H

D

V

V

S

T

A
|
A

N
|
N

K

K

G

S

P

G

L

L

A

A

V

N

S

F

F

W

K

P

D

E

L

I

V

M

K

E

C

Y

A

A

K

R

E

S

N

N

K

N

V

R

C

R

F

I

R

R

Y

Y

E

E

A
|
A

S

T

V

V

G

A

G

G

A

G

M

V

P

P

I

L

I

F

N

S

L

F

A

I

K

D

E

Y

T

S

L

V

A

L

G

P

N

S

D

R

I

I

K

K

L

K

I

F

K

R

G

G

I

I

L

V

N

S

G

L

T

T

T

I

N

N

Y

Y

I

F

L

I

T

R

K

E

M

L

E

A

K

N

E

K

Q

R

L

-

D

E

F

F

D

D

T

D

V

V

L

L

K

S

E

E

A

A

Q

T

E

K

L

L

G

G

I

I

A

-

E

-

T

V

N

N

P

Y

H

K

Q

D

D

D

I

L

S

T

G

G

L

L

D
|
D

T

A

A

A

A

R

K
|
K

I

S

V

V

I

I

L

L

A

C

N

N

S

H

I

L

F

Y

G

G

R

S

D

S

V

Y

T

R

I

L

K

S

D

D

V

V

N

F

L

Y

E

E

G

G

I

I

T

-

R

-

I

-

T

-

P

-

E

-

A

-

L

-

A

-

M

L

A

D

N

R

K

S

S

F

G

G

H

K

T

N

I

E

K

R

L

L

I

V

G

T

E

E

-

T

-

G

V

I

T

V

D

N

K

G

K

K

L

P

E

S

V

A

C

E

P

S

K

R

L

I

I

K

P

S

I

L

D

D

S

S

P

N

L

D

N

Y

V

L

-

L

-

T

-

L

-

G

K

K

G

G

S

S

L

L

N

G

V

Y

A

Q

M

L

V

-

N

Q

T

T

D

D

L

T

A

N

N

G

D

T

I

L

V

N

V

V

V

S

G

D

A

L

G

Y

A

D

G

G

D

P

I

Y

E

E

T
|
T

A

A

S

G

A

A

I

V

L

M

S

N

D

D

L

L

V

V

active site: D212 (= D220), K216 (= K224)
binding nicotinamide-adenine-dinucleotide: G10 (= G7), L11 (≠ F8), G12 (= G9), N13 (≠ I10), V14 (≠ I11), D42 (= D41), S43 (≠ R42), A90 (≠ V94), T91 (≠ S95), P92 (= P96), A117 (= A122), N118 (= N123), A144 (= A149), T308 (= T320)

6a0tB Homoserine dehydrogenase k99a mutant from thermus thermophilus hb8 complexed with hse and NADP+ (see paper)
37% identity, 54% coverage: 109:289/337 of query aligns to 84:264/332 of 6a0tB

query
sites
6a0tB

M

V

L

L

K

P

A

A

F

L

E

E

C

A

K

G

K

I

H

P

V

L

V

I

S

T

A
|
A

N
|
N

K

K

G

A

P

L

L

L

A

A

V

E

S

A

F

W

K

E

D

S

L

L

V

R

K

P

C

F

A

A

K

E

E

E

N

G

K

L

V

I

C

Y

F

-

R

-

Y

H

E

E

A

A

S

S

V

V

G

M

G

A

A

G

M

T

P

P

I

A

I

L

N

S

L

F

A

L

K

-

E

E

T

T

L

L

A

R

G

G

N

S

D

E

I

L

K

L

L

E

I

L

K

H

G

G

I

I

L

L

N
|
N

G
|
G

T
|
T

T

T

N

L

Y

Y

I

I

L

L

T

Q

K

E

M

M

E

E

K

K

E

G

Q

R

L

-

D

T

F

Y

D

A

T

E

V

A

L

L

K

L

E

E

A

A

Q

Q

E

R

L

L

G
|
G

I
x
Y

A

A

E
|
E

T

A

N

D

P

P

H

T

Q

L

D
|
D

I

V

S

E

G

G

L

I

D
|
D

T

A

A

A

A

H

K
|
K

I

L

V

T

I

L

L

L

A

A

N

R

S

L

I

L

F

V

G

D

R

P

D

G

V

F

T

P

I

F

K

A

D

E

V

V

N

E

L

A

E

Q

G

G

I

I

T

A

R

R

I

L

T

T

P

P

E

E

A

V

L

L

A

Q

M

K

A

A

N

E

K

A

S

R

G

G

H

E

T

R

I

V

K

R

L

L

I

V

-

A

-

S

-

L

-

F

G

G

E

E

V

G

T

G

D

R

K

W

K

R

L

A

E

A

V

V

C

A

P

P

K

R

L

R

I

L

P

P

I

Q

D

D

S

H

P

P

L

L

active site: D191 (= D220), K195 (= K224)
binding l-homoserine: N150 (= N178), G151 (= G179), T152 (= T180), Y178 (≠ I207), E180 (= E209), D186 (= D215), K195 (= K224)
binding nadp nicotinamide-adenine-dinucleotide phosphate: A97 (= A122), N98 (= N123), G177 (= G206), E180 (= E209)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 10, 11, 12, 13, 14, 42, 43, 44, 45, 48, 50, 73, 74, 75, 289, 290, 294

6a0sA Homoserine dehydrogenase from thermus thermophilus hb8 complexed with hse and NADPH (see paper)
37% identity, 54% coverage: 109:289/337 of query aligns to 84:264/331 of 6a0sA

query
sites
6a0sA

M

V

L

L

K

P

A

A

F

L

E

E

C

A

K

G

K

I

H

P

V

L

V

I

S

T

A
|
A

N

N

K
|
K

G

A

P

L

L

L

A

A

V

E

S

A

F

W

K

E

D

S

L

L

V

R

K

P

C

F

A

A

K

E

E

E

N

G

K

L

V

I

C

Y

F

-

R

-

Y

H

E

E

A

A

S

S

V

V

G

M

G

A

A

G

M

T

P

P

I

A

I

L

N

S

L

F

A

L

K

-

E

E

T

T

L

L

A

R

G

G

N

S

D

E

I

L

K

L

L

E

I

L

K

H

G

G

I

I

L

L

N
|
N

G
|
G

T
|
T

T

T

N

L

Y

Y

I

I

L

L

T

Q

K

E

M

M

E

E

K

K

E

G

Q

R

L

-

D

T

F

Y

D

A

T

E

V

A

L

L

K

L

E

E

A

A

Q

Q

E

R

L

L

G
|
G

I
x
Y

A

A

E
|
E

T

A

N

D

P

P

H

T

Q

L

D
|
D

I

V

S

E

G

G

L

I

D
|
D

T

A

A

A

A

H

K
|
K

I

L

V

T

I

L

L

L

A

A

N

R

S

L

I

L

F

V

G

D

R

P

D

G

V

F

T

P

I

F

K

A

D

E

V

V

N

E

L

A

E

Q

G

G

I

I

T

A

R

R

I

L

T

T

P

P

E

E

A

V

L

L

A

Q

M

K

A

A

N

E

K

A

S

R

G

G

H

E

T

R

I

V

K

R

L

L

I

V

-

A

-

S

-

L

-

F

G

G

E

E

V

G

T

G

D

R

K

W

K

R

L

A

E

A

V

V

C

A

P

P

K

R

L

R

I

L

P

P

I

Q

D

D

S

H

P

P

L

L

active site: D191 (= D220), K195 (= K224)
binding l-homoserine: K99 (= K124), N150 (= N178), G151 (= G179), T152 (= T180), Y178 (≠ I207), E180 (= E209), D186 (= D215), K195 (= K224)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A97 (= A122), K99 (= K124), G177 (= G206), E180 (= E209)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 11, 12, 13, 14, 42, 43, 44, 45, 48, 50, 73, 74, 289, 290, 294

2ejwA Homoserine dehydrogenase from thermus thermophilus hb8
37% identity, 54% coverage: 109:289/337 of query aligns to 84:264/331 of 2ejwA

query
sites
2ejwA

M

V

L

L

K

P

A

A

F

L

E

E

C

A

K

G

K

I

H

P

V

L

V

I

S

T

A

A

N

N

K

K

G

A

P

L

L

L

A

A

V

E

S

A

F

W

K

E

D

S

L

L

V

R

K

P

C

F

A

A

K

E

E

E

N

G

K

L

V

I

C

Y

F

-

R

-

Y

H

E
|
E

A

A

S

S

V
|
V

G
x
M

G
x
A

A

G

M
x
T

P

P

I

A

I

L

N

S

L

F

A

L

K

-

E

E

T

T

L

L

A

R

G

G

N

S

D

E

I

L

K

L

L

E

I

L

K

H

G

G

I

I

L

L

N

N

G

G

T

T

N

L

Y

Y

I

I

L

L

T

Q

K

E

M

M

E

E

K

K

E

G

Q

R

L

-

D

T

F

Y

D

A

T

E

V

A

L

L

K

L

E

E

A

A

Q

Q

E

R

L

L

G

G

I

Y

A

A

E

E

T

A

N

D

P

P

H

T

Q

L

D

D

I

V

S

E

G

G

L

I

D
|
D

T

A

A

A

A

H

K
|
K

I

L

V

T

I

L

L

L

A

A

N

R

S

L

I

L

F

V

G

D

R

P

D

G

V

F

T

P

I

F

K

A

D

E

V

V

N

E

L

A

E

Q

G

G

I

I

T

A

R

R

I

L

T

T

P

P

E

E

A

V

L

L

A

Q

M

K

A

A

N

E

K

A

S

R

G

G

H

E

T

R

I

V

K

R

L

L

I

V

-

A

-

S

-

L

-

F

G

G

E

E

V

G

T

G

D

R

K

W

K

R

L

A

E

A

V

V

C

A

P

P

K

R

L

R

I

L

P

P

I

Q

D

D

S

H

P

P

L

L

active site: D191 (= D220), K195 (= K224)
binding magnesium ion: E121 (= E148), V124 (= V151), M125 (≠ G152), A126 (≠ G153), T128 (≠ M155)

O81852 Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic; AK-HD 2; AK-HSDH 2; Beta-aspartyl phosphate homoserine 2; EC 2.7.2.4; EC 1.1.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
26% identity, 98% coverage: 5:335/337 of query aligns to 562:912/916 of O81852

query
sites
O81852

L

I

V

V

G

G

F

P

G

G

I

L

I

I

G

G

K

A

G

T

V

L

L

L

K

D

T

Q

I

L

T

R

L

D

K

Q

S

A

E

A

H

V

L

L

K

K

K

Q

R

E

Y

F

G

N

M

I

D

D

L

L

Q

R

V

V

A

L

A

G

I

I

C

T

D

G

R

S

S

K

G

K

A

M

A

L

I

L

D

S

E

D

N

I

G

G

L

I

D

D

L

L

E

S

L

R

A

W

L

R

K

E

I

L

K

L

E

N

E

E

T

-

G

-

K

-

I

-

A

-

N

-

Y

-

P

-

E

-

K

K

G

G

C

T

E

E

M

A

G

D

I

L

L

D

E

K

V

F

I

T

E

Q

S

Q

V

V

S

H

A

G

D

N

A

H

V

F

V

I

E

P

V

N

S

S

P

V

T

V

N

V

I

D

E

C

T

T

G

A

E

D

P

S

A

A

-

I

K

A

S

S

Y

R

M

Y

L

Y

K

D

A

W

F

L

E

R

C

K

K

G

K

I

H

H

V

V

V

I

S

T

A

P

N

N

K

K

-

K

-

A

-

N

-

S

G

G

P

P

L

L

A

D

V

Q

S

Y

F

L

K

K

D

-

L

L

V

R

K

D

C

L

A

Q

K

R

E

K

N

S

K

Y

V

T

C

H

F

Y

R

F

Y

Y

E

E

A

A

S

T

V

V

G

G

G

A

A

G

M

L

P

P

I

I

N

S

L

T

A

L

K

R

E

G

T

L

L

L

-

E

A

T

G

G

N

D

D

K

I

I

K

L

L

R

I

I

K

E

G

G

I

I

L

C

N

S

G

G

T

T

T

L

N

S

Y

Y

I

L

L

F

T

N

K

N

M

F

E

V

K

G

E

D

Q

R

L

-

D

S

F

F

D

S

T

E

V

V

L

V

K

T

E

E

A

A

Q

K

E

N

L

A

G

G

I

F

A

T

E

E

T

P

N

D

P

P

H

R

Q

D

D

D

I

L

S

S

G

G

L

T

D

D

T

V

A

A

A

R

K

K

I

V

V

I

I

I

L

L

A

A

N

-

S

-

I

-

F

-

G

-

R

R

D

E

V

S

T

G

I

L

K

K

-

L

D

D

V

L

N

A

L

D

E

L

G

P

I

I

T

R

R

S

I

L

T

V

P

P

E

E

A

P

L

L

A

K

M

G

-

C

-

T

-

S

-

V

-

E

-

F

-

M

-

E

-

K

-

L

-

P

-

Q

-

Y

-

D

-

G

-

D

-

L

-

A

-

K

-

E

-

R

-

L

-

D

A

A

N

E

K

N

S

S

G

G

H

E

T

V

I

L

K

R

L

Y

I

V

G

G

-

V

E

D

V

A

T

V

D

N

K

Q

K

K

L

G

E

T

V

V

C

E

P

L

K

R

L

R

I

Y

P

K

I

K

D

E

S

H

P

P

L

F

-

A

N

Q

V

L

K

A

G

G

S

S

L

D

N

N

V

I

A

I

M

A

V

F

N

T

T

T

D

T

L

R

A

Y

N

K

D

D

-

H

-

P

I

L

V

I

V

V

V

R

G

G

A

P

G

G

A

A

G

G

D

A

I

Q

E

V

T

T

A

A

S

G

A

G

I

I

L

F

S

S

D

D

L

I

V

L

N

R

I

L

H

A

Q

S

T

Y

L

L

Sites not aligning to the query:

441 I→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
443 Q→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
522 I→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.
524 Q→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.

O94671 Homoserine dehydrogenase; HDH; HSD; EC 1.1.1.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
34% identity, 37% coverage: 105:229/337 of query aligns to 105:236/376 of O94671

query
sites
O94671

A

A

K

Q

S

S

Y

Y

M

P

L

-

K

K

A

F

F

L

E

S

C

K

K

K

K

I

H

N

V

I

V

A

S

T

A

P

N

N

K

K

G

K

P

A

L

F

A

S

V

A

S

S

-

N

-

D

-

V

F

Y

K

Q

D

N

L

I

V

I

K

K

C

A

A

S

K

K

E

E

N

S

K

G

V

A

C

L

F

L

R

M

Y

H

E

E

A

A

S

S

V

V

G

G

G

A

A

G

M

L

P

P

I

I

N

S

L

T

A

L

K

K

E

E

T

L

L

I

A

A

-

T

G

G

N

D

D

E

I

I

K

I

L

K

I

I

K

E

G

G

I

I

L

F

N

S

G

G

T

T

T

L

N

S

Y

Y

I

I

L

F

T

N

K

V

M

W

-

S

-

P

-

N

-

G

E

K

K

K

E

G

Q

T

L

A

D
x
S

F

F

D

S

T

D

V

I

L

V

K

K

E

I

A

A

Q

K

E

Q

L

N

G

G

I

Y

A

T

E

E

T

P

N

D

P

P

H

R

Q

D

D

D

I

L

S

N

G

G

L

M

D

D

T

V

A

A

A

R

K

K

I

V

V

T

I

I

L

L

A

S

S201 (≠ D194) modified: Phosphoserine

P31116 Homoserine dehydrogenase; HDH; HSD; EC 1.1.1.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
34% identity, 29% coverage: 130:228/337 of query aligns to 126:227/359 of P31116

query
sites
P31116

S

T

F

W

K

K

D

A

L

L

V

F

K

S

C

N

A

K

K

P

E

T

N

N

K

G

V

F

C

V

F

Y

R

-

Y

H

E
|
E

A

A

S

T

V
|
V

G

G

G
x
A

A

G

M
x
L

P

P

I

I

N

S

L

F

A

L

K

R

E

E

T

I

L

I

-

Q

A

T

G

G

N

D

D

E

I

V

K

E

L

K

I

I

K

E

G

G

I

I

L

F

N

S

G

G

T

T

T

L

N

S

Y

Y

I

I

L

F

T

N

K

E

M

F

E

S

K

T

E

S

Q

Q

-

A

-

N

-

D

L

V

D

K

F

F

D

S

T

D

V

V

L

V

K

K

E

V

A

A

Q

K

E

K

L

L

G

G

I

Y

A

T

E
|
E

T

P

N

D

P

P

H

R

Q

D

D

D

I

L

S

N

G

G

L

L

D
|
D

T

V

A

A

A

R

K
|
K

I

V

V

T

I

I

L

V

E143 (= E148) binding Na(+)
V146 (= V151) binding Na(+)
A148 (≠ G153) binding Na(+)
L150 (≠ M155) binding Na(+)
E208 (= E209) binding L-homoserine; mutation to D: Increases KM for aspartate-semialdehyde 48-fold and reduces kcat by 50%.; mutation E->L,Q: Loss of activity.
D219 (= D220) binding L-homoserine; mutation to L: Reduces kcat 150-fold.
K223 (= K224) mutation to V: Loss of activity.

Sites not aligning to the query:

13 binding NAD(+)
15 binding NAD(+)
16 binding NAD(+)
41 binding NAD(+)
79 H→A: Reduces kcat 2-fold.
93 binding NAD(+)
309 H→A: Reduces kcat 40-fold. Affects dimer formation.
340 binding NAD(+)

1tveA Homoserine dehydrogenase in complex with 4-(4-hydroxy-3- isopropylphenylthio)-2-isopropylphenol (see paper)
34% identity, 29% coverage: 130:228/337 of query aligns to 125:226/358 of 1tveA

query
sites
1tveA

S

T

F

W

K

K

D

A

L

L

V

F

K

S

C

N

A

K

K

P

E

T

N

N

K

G

V

F

C

V

F

Y

R

-

Y

H

E

E

A

A

S

T

V

V

G

G

G

A

A

G

M

L

P

P

I

I

N

S

L

F

A

L

K

R

E

E

T

I

L

I

-

Q

A

T

G

G

N

D

D

E

I

V

K

E

L

K

I

I

K

E

G

G

I

I

L

F

N

S

G

G

T
|
T

T

L

N

S

Y

Y

I

I

L

F

T

N

K

E

M

F

E

S

K

T

E

S

Q

Q

-

A

-

N

-

D

L

V

D

K

F

F

D

S

T

D

V

V

L

V

K

K

E

V

A

A

Q

K

E

K

L

L

G

G

I

Y

A

T

E

E

T

P

N

D

P

P

H

R

Q

D

D
|
D

I

L

S

N

G

G

L

L

D
|
D

T

V

A

A

A

R

K
|
K

I

V

V

T

I

I

L

V

active site: D218 (= D220), K222 (= K224)
binding 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol: T175 (= T180), D213 (= D215), D218 (= D220)

Sites not aligning to the query:

binding 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol: 93, 116

1q7gA Homoserine dehydrogenase in complex with suicide inhibitor complex NAD-5-hydroxy-4-oxonorvaline (see paper)
34% identity, 29% coverage: 130:228/337 of query aligns to 125:226/358 of 1q7gA

query
sites
1q7gA

S

T

F

W

K

K

D

A

L

L

V

F

K

S

C

N

A

K

K

P

E

T

N

N

K

G

V

F

C

V

F

Y

R

-

Y

H

E

E

A
|
A

S

T

V

V

G

G

G

A

A

G

M

L

P

P

I

I

N

S

L

F

A

L

K

R

E

E

T

I

L

I

-

Q

A

T

G

G

N

D

D

E

I

V

K

E

L

K

I

I

K

E

G

G

I

I

L

F

N
x
S

G

G

T

T

T

L

N

S

Y

Y

I

I

L

F

T

N

K

E

M

F

E

S

K

T

E

S

Q

Q

-

A

-

N

-

D

L

V

D

K

F

F

D

S

T

D

V

V

L

V

K

K

E

V

A

A

Q

K

E

K

L

L

G

G

I

Y

A

T

E

E

T

P

N

D

P

P

H

R

Q

D

D

D

I

L

S

N

G

G

L

L

D
|
D

T

V

A

A

A

R

K
|
K

I

V

V

T

I

I

L

V

active site: D218 (= D220), K222 (= K224)
binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: A143 (= A149), S173 (≠ N178), K222 (= K224)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: 13, 14, 15, 39, 91, 92, 93, 97, 114, 116, 338, 343

1ebuD Homoserine dehydrogenase complex with NAD analogue and l-homoserine (see paper)
34% identity, 29% coverage: 130:228/337 of query aligns to 125:226/358 of 1ebuD

query
sites
1ebuD

S

T

F

W

K

K

D

A

L

L

V

F

K

S

C

N

A

K

K

P

E

T

N

N

K

G

V

F

C

V

F

Y

R

-

Y

H

E

E

A

A

S

T

V

V

G

G

G

A

A

G

M

L

P

P

I

I

N

S

L

F

A

L

K

R

E

E

T

I

L

I

-

Q

A

T

G

G

N

D

D

E

I

V

K

E

L

K

I

I

K

E

G

G

I

I

L

F

N

S

G

G

T

T

T

L

N

S

Y

Y

I

I

L

F

T

N

K

E

M

F

E

S

K

T

E

S

Q

Q

-

A

-

N

-

D

L

V

D

K

F

F

D

S

T

D

V

V

L

V

K

K

E

V

A

A

Q

K

E

K

L

L

G

G

I

Y

A

T

E

E

T

P

N

D

P

P

H

R

Q

D

D

D

I

L

S

N

G

G

L

L

D
|
D

T

V

A

A

A

R

K
|
K

I

V

V

T

I

I

L

V

active site: D218 (= D220), K222 (= K224)

Sites not aligning to the query:

binding 3-aminomethyl-pyridinium-adenine-dinucleotide: 11, 12, 13, 14, 15, 39, 40, 91, 93, 116, 343

Query Sequence

>WP_011869448.1 NCBI__GCF_000016125.1:WP_011869448.1
MKIILVGFGIIGKGVLKTITLKSEHLKKRYGMDLQVAAICDRSGAAIDENGLDLELALKI
KEETGKIANYPEKGCEMGILEVIESVSADAVVEVSPTNIETGEPAKSYMLKAFECKKHVV
SANKGPLAVSFKDLVKCAKENKVCFRYEASVGGAMPIINLAKETLAGNDIKLIKGILNGT
TNYILTKMEKEQLDFDTVLKEAQELGIAETNPHQDISGLDTAAKIVILANSIFGRDVTIK
DVNLEGITRITPEALAMANKSGHTIKLIGEVTDKKLEVCPKLIPIDSPLNVKGSLNVAMV
NTDLANDIVVVGAGAGDIETASAILSDLVNIHQTLKN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory