SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011876163.1 NCBI__GCF_000016205.1:WP_011876163.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
31% identity, 97% coverage: 9:455/459 of query aligns to 4:439/443 of 4lnkA

query
sites
4lnkA

T

T

A

R

A

E

D

D

A

I

R

E

K

K

I

L

V

V

E

K

E

E

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Y

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Q

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F

T

T

D

D

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I

D

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N

K

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Y

E

L

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S

P

R

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D

S

K

Q

F

L

F

G

S
x
K

A

A

L

L

E

D

N

N

S

K

F

V

G
x
M

F

F

-
x
D

C

G

S

S

V

S

L

I

F

E

G

G

W

F

D

-

C

-

N

-

D

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E

-

L

-

Y

-

D

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E

-

A

-

C

V

K

R

I

I

E

E

Y

E

T

S

G

D

W

M

H

Y

N

-

G

L

Y

Y

R

P

D

D

D

L

E

N

V

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F

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V

I

I

P

F

E

P

S

W

C

T

R

A

E

E

L

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P

-

H

K

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N

K

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L

A

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R

F

F

L

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A

C

E

D

A

I

H

Y

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N

E

P

H

D

G

G

A

T

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P

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F

-

E

-

G

C

D

P

P

R

R

N

N

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N

L

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K

R

R

V

I

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A

K

R

E

A

M

D

E

E

D

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G

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S

N

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L

G

G

F

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E
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E

Y

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E
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E

F

F

Y

F

G

L

F

F

N

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E

-

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K

A

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A

D

E

E

K

K

G

G

Y

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R

P

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T

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L

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M

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E

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x
F

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Y

D

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H

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S

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K

K

D

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A

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D

A

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A

N

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Y

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M

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K

-

E

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-

M

-

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N

G

G

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V

T

D

L

L

P

P

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A

T

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L

L

A

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E

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A

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G

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F

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K

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N

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G

D

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F

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T

A

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K

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I

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W

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M

Y

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Y

active site: D52 (vs. gap), E131 (= E145), E133 (= E147), E188 (= E208), E195 (= E215), H244 (= H264), R315 (= R336), E332 (= E353), R334 (= R355)
binding adenosine-5'-diphosphate: K43 (≠ S48), M50 (≠ G55), F198 (≠ L218), Y200 (≠ L220), N246 (≠ H266), S248 (= S268), S324 (≠ G345), S328 (= S349), R330 (= R351)
binding glutamic acid: E133 (= E147), E188 (= E208), V189 (≠ A209), N239 (≠ P259), G240 (= G260), G242 (= G262), E303 (≠ W324)
binding magnesium ion: E131 (= E145), E188 (= E208), E195 (= E215), H244 (= H264), E332 (= E353)

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
31% identity, 97% coverage: 9:455/459 of query aligns to 4:439/443 of 4lniA

query
sites
4lniA

T

T

A

R

A

E

D

D

A

I

R

E

K

K

I

L

V

V

E

K

E

E

R

E

G

N

L

V

T

K

H

Y

V

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E

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L

A

Q

V

F

T

T

D

D

L

I

D

L

G

G

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G

N

K

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Y

E

L

I

S

P

R

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D

S

K

Q

F

L

F

G

S

K

A

A

L

L

E

D

N

N

S

K

F

V

G

M

F

F

-
x
D

C

G

S

S

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S

L

I

F

E

G

G

W

F

D

-

C

-

N

-

D

-

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L

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Y

-

D

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E

-

A

-

C

V

K

R

I

I

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Y

E

T

S

G

D

W

M

H

Y

N

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G

L

Y

Y

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D

D

D

L

E

N

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F

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I

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F

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H

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G

G

A

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P

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R

N

N

L

N

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K

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I

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A

M

D

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E

D

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L

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G

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E
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E

Y

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E

F

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F

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K

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E

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E

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E

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x
D

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D

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S

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K

K

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L

L

A

A

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A

G

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D

G

G

I

I

E

K

H

N

E

K

L

L

E

E

P

A

T

P

P

A

P

P

S

I

T

D

G

R

N

N

A

I

Y

Y

V

V

Q

M

K

S

V

K

-

E

-

R

-

M

-

E

S

N

G

G

E

I

L

V

T

D

L

L

P

P

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A

T

T

L

L

A

A

E

E

A

A

A

L

G

E

R

E

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F

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K

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S

N

R

E

A

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D

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C

A

F

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G

G

D

E

A

H

F

L

V

F

D

E

H

H

F

F

A

I

S

E

T

A

R

K

E

E

W

I

E

E

D

W

R

D

E

M

Y

F

R

R

R

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H

Q

V

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S

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D

P

W

W

E

E

L

R

K

E

R

Q

Y

Y

active site: D52 (vs. gap), E131 (= E145), E133 (= E147), E188 (= E208), E195 (= E215), H244 (= H264), R315 (= R336), E332 (= E353), R334 (= R355)
binding adenosine-5'-diphosphate: E131 (= E145), E183 (= E203), D197 (≠ A217), Y200 (≠ L220), N246 (≠ H266), S248 (= S268), R320 (= R341), R330 (= R351)
binding magnesium ion: E131 (= E145), E131 (= E145), E133 (= E147), E188 (= E208), E195 (= E215), E195 (= E215), H244 (= H264), E332 (= E353)
binding l-methionine-s-sulfoximine phosphate: E133 (= E147), E188 (= E208), H244 (= H264), R297 (= R318), E303 (≠ W324), R315 (= R336), R334 (= R355)

P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 5 papers)
31% identity, 97% coverage: 9:455/459 of query aligns to 5:440/444 of P12425

query
sites
P12425

T

T

A

R

A

E

D

D

A

I

R

E

K

K

I

L

V

V

E

K

E

E

R

E

G

N

L

V

T

K

H

Y

V

I

E

R

V

L

A

Q

V

F

T

T

D

D

L

I

D

L

G

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R

K

G

N

K

V

Y

E

L

I

S

P

R

V

D

S

K

Q

F

L

F

G

S

K

A

A

L

L

E

D

N

N

S

K

F

V

G

M

F

F

-

D

C

G

S

S

V

S

L

I

F

E

G
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G

W

F

D

-

C

-

N

-

D

-

E

-

L

-

Y

-

D

-

E

-

A

-

C

V

K
x
R

I

I

E

E

Y

E

T

S

G

D

W

M

H

Y

N

-

G

L

Y

Y

R

P

D

D

D

L

E

N

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V

I

I

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F

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C

T

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A

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G

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N

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N

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M

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F

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F

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H

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S

L

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K

K

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P

N

V

A

F

F

D

D

A

E

A

N

Q

A

A

D

H

L

Q

Q

M

L

S

S

K

E

T

T

M

A

R

K

H

H

F

F

I

I

A

A

G

G

Q

I

Q

V

L

K

L

H

M

A

P

T

E

S

F

F

L

T

A

A

M

V

V

T

A

N

P

P

T

T

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V

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N

A

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Y

Y

S

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R

R

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L

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G

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Y

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x
E

A

A

P
|
P

T

C

S

Y

A

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T

A

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W

G

S

V

A

D

Q

N

N

R

R

T

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C

P

A

L

L

I

R

R

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I

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G

A

G

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S

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E
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E

Y

V

R

R

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S

D

A

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D

A

T

A

N

N

P

P

Y

Y

L

L

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A

Q

L

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S

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L

L

L

A

A

S

A

G

G

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L

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D

G

G

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H

N

E

K

L

L

E

E

P

A

T

P

P

A

P

P

S

I

T

D

G

R

N

N

A

I

Y

Y

V

V

Q

M

K

S

V

K

-

E

-

R

-

M

-

E

S

N

G

G

E

I

L

V

T

D

L

L

P

P

R

A

T

T

L

L

A

A

E

E

A

A

A

L

G

E

R

E

L

F

R

K

S

S

S

N

R

E

A

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A

M

R

V

D

K

C

A

F

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G

G

D

E

A

H

F

L

V

F

D

E

H

H

F

F

A

I

S

E

T

A

R

K

E

E

W

I

E
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E

D

W

R

D

E

M

Y

F

R

R

R

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Y

Y

G59 (= G62) mutation to R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
R62 (≠ K75) Important for inhibition by glutamine; mutation to A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.
E132 (= E145) binding Mg(2+)
E134 (= E147) binding Mg(2+)
E189 (= E208) binding Mg(2+)
V190 (≠ A209) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E215) binding Mg(2+)
G241 (= G260) binding L-glutamate
H245 (= H264) binding Mg(2+)
G302 (= G322) mutation to E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
E304 (≠ W324) mutation to A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
P306 (= P326) mutation to H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
E333 (= E353) binding Mg(2+)
E424 (= E439) mutation to K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4s0rD Structure of gs-tnra complex (see paper)
31% identity, 97% coverage: 9:455/459 of query aligns to 8:443/447 of 4s0rD

query
sites
4s0rD

T

T

A

R

A

E

D

D

A

I

R

E

K

K

I

L

V

V

E

K

E

E

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D

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N

K

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E

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R

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D

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K

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F

L

F

G

S

K

A

A

L

L

E

D

N

N

S

K

F

V

G

M

F

F

-
x
D

C

G

S

S

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S

L

I

F

E

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G

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x
F

D

-

C

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N

-

D

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L

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-

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x
V

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x
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I
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I

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Y

E

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K

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E

P

H

D

G

G

A

T

R

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F

-

E

-

G

C

D

P

P

R

R

N

N

L

N

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R

V

I

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R

E

A

M

D

E

E

D

L

L

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G

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D

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S

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E
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E

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E

F

F

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F

G

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E

E

K

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D

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G

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R

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Y

active site: D56 (vs. gap), E135 (= E145), E137 (= E147), E192 (= E208), E199 (= E215), H248 (= H264), R319 (= R336), E336 (= E353), R338 (= R355)
binding glutamine: E137 (= E147), E192 (= E208), R301 (= R318), E307 (≠ W324)
binding magnesium ion: I66 (= I76), E135 (= E145), E135 (= E145), E199 (= E215), H248 (= H264), H248 (= H264), E336 (= E353), H419 (= H431)
binding : F63 (≠ W63), V64 (≠ C74), R65 (≠ K75), I66 (= I76), D161 (vs. gap), G241 (≠ E257), V242 (≠ M258), N243 (≠ P259), G305 (= G322), Y306 (= Y323), Y376 (= Y393), I426 (≠ W438), M430 (≠ E442)

5dm3C Crystal structure of glutamine synthetase from chromohalobacter salexigens dsm 3043(csal_0679, target efi-550015) with bound adp
29% identity, 97% coverage: 12:457/459 of query aligns to 4:396/396 of 5dm3C

query
sites
5dm3C

D

D

A

L

R

K

K

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E

E

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R

A

D

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H

F

F

F

-

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-

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F

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G

G

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W

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E

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Y

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D

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D

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C

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G

Y

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R

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D

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E

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A

R

M

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V

I

L

P

-

E

-

S

-

C

C

R

D

E

L

L

L

P

D

H

H

K

R

N

T

S

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L

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F

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L

-

A

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H

A

A

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E

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E

P

H

H

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A

A

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A

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E
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E

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E

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A

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E

A

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L

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x
R

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x
C

D

A

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A

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H

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S

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K

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P

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R

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A

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G

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-

A

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G

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N

L

L

A

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D

A

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G

E

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A

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L

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C

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A

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F

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F

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E

active site: E115 (= E145), E117 (= E147), E162 (= E208), E169 (= E215), H218 (= H264), R286 (= R336), E303 (= E353), R305 (= R355)
binding adenosine-5'-diphosphate: R173 (≠ S219), C174 (≠ L220), H220 (= H266), S222 (= S268), R301 (= R351)

8ufjB Glutamine synthetase (see paper)
28% identity, 98% coverage: 9:459/459 of query aligns to 6:444/444 of 8ufjB

query
sites
8ufjB

T

T

A

K

A

E

D

D

A

V

R

L

K

E

I

A

V

V

E

K

E

E

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A

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F

L

F

E

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E

A

A

L

F

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E

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M

F

F

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C

G

S

S

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S

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G

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F

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C

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N

-

D

-

E

-

L

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Y

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A

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|
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A

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V

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A

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W

M

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W

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D

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Y

Y

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K

R

A

H

V

V

V

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H

D

P

W

W

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R

Y

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M

I

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binding magnesium ion: E132 (= E145), E134 (= E147), E189 (= E208), E196 (= E215), H245 (= H264), E333 (= E353)

P78061 Gamma-glutamylputrescine synthetase PuuA; Gamma-Glu-Put synthetase; Glutamate--putrescine ligase; EC 6.3.1.11 from Escherichia coli (strain K12) (see paper)
30% identity, 94% coverage: 24:453/459 of query aligns to 39:466/472 of P78061

query
sites
P78061

H

Y

V

V

E

D

V

V

A

L

V

L

T

T

D

D

L

L

D

N

G

G

V

C

L

F

R

R

G

G

K

K

Y

R

L

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P

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D

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L

F

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S

K

A

K

L

L

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E

N

K

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G

F

C

G

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F

F

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A

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S

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V

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F

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A

W

M

D

D

C

I

N

L

D

G

E

N

L

V

Y

V

D

E

E

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A

A

C

-

K

-

I

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Y

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T

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G

G

W

L

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G

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N

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A

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F

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L

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D

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P

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Q

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A

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A

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G

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|
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M

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P

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F

S

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M

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A

M

L

V

L

A

A

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P

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N

V

V

N

N

A

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Y

Y

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R

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G

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M

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Y

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P

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T

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A

A

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S

W

W

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G

V

H

D

N

N

N

R

R

T

T

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A

A

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|
R

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I

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C

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K

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V

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N

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P

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L

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G

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L

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G

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N

A

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Q

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-

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G

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R

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A

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A

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G

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F

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H

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A

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N

W

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H282 (= H264) mutation to N: Activity is impaired to 9% of wild-type.
R357 (= R341) mutation to Q: Activity is impaired to 3% of wild-type.

8tfkA Glutamine synthetase (see paper)
28% identity, 98% coverage: 9:459/459 of query aligns to 2:440/440 of 8tfkA

query
sites
8tfkA

T

T

A

K

A

E

D

D

A

V

R

L

K

E

I

A

V

V

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N

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G

G

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M

V

N

S

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G

G

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P

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|
E

Y

L

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|
E

F

F

Y

F

G

L

F

F

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K

-

L

-

D

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A

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N

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N

N

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M

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|
E

A

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G

A

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P

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H

E
|
E

A

I

A
x
D

L
x
F

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L
x
F

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G

T

D

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A

A

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|
G

C

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G

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H
|
H

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H
x
N

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K

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R

K

R

A

H

V

V

V

S

H

D

P

W

W

E

E

L

L

K

S

R

R

Y

Y

F

L

E

S

I

M

I

L

binding adenosine-5'-diphosphate: E128 (= E145), D194 (≠ A217), F195 (≠ L218), F197 (≠ L220), N243 (≠ H266), R312 (= R336), R317 (= R341), G325 (≠ S349), R327 (= R351)
binding magnesium ion: E128 (= E145), E128 (= E145), E130 (= E147), E185 (= E208), E192 (= E215), E192 (= E215), H241 (= H264), E329 (= E353)
binding l-methionine-s-sulfoximine phosphate: E128 (= E145), E130 (= E147), E185 (= E208), E192 (= E215), G237 (= G260), H241 (= H264), R294 (= R318), E300 (≠ W324), R312 (= R336), R331 (= R355)

7tdpA Structure of paenibacillus polymyxa gs bound to met-sox-p-adp (transition state complex) to 1.98 angstom (see paper)
30% identity, 98% coverage: 9:458/459 of query aligns to 3:438/439 of 7tdpA

query
sites
7tdpA

T

T

A

R

A

E

D

D

A

I

R

I

K

R

I

I

V

A

E

E

R

E

G

N

L

V

T

R

H

F

V

I

E

R

V

L

A

Q

V

F

T

T

D

D

L

L

D

L

G

G

V

T

L

I

R

K

G

N

K

V

Y

E

L

I

S

P

R

V

D

S

K

Q

F

L

F

E

S

K

A

A

L

L

E

D

N

N

S

K

F

M

G

M

F

F

-

D

C

G

S

S

V

S

L

I

F

E

G

G

W

Y

D

-

C

-

N

-

D

-

E

-

L

-

Y

-

D

-

E

-

A

-

C

V

K

R

I

I

E

E

Y

E

T

S

G

D

W

M

H

Y

N

L

G

Y

Y

P

R

D

D

L

D

D

E

T

V

W

R

V

V

V

I

F

P

P

E

W

S

S

C

D

R

R

E

-

L

-

P

-

H

-

K

-

N

-

S

V

L

A

L

R

F

L

L

I

A

C

E

D

A

I

H

Y

S

K

E

P

H

D

G

G

A

S

R

P

V

F

C

A

-

G

-

D

P

P

R

R

N

G

L

I

L

L

S

K

R

R

V

V

L

L

A

K

R

E

A

A

D

E

E

E

L

L

G

G

F

Y

S

T

V

S

V

M

S
x
N

-

V

G
|
G

F

P

E
|
E

Y

P

E
|
E

F

F

Y

F

G

L

F

F

-

K

-

T

-

D

-

E

-

K

-

G

N

D

E

P

T

T

P

T

Q

E

S

L

A

N

A

D

E

Q

K

G

G

G

Y

Y

R

F

D

D

L

L

K

-

L

-

T

A

P

P

T

M

S

D

C

L

G

G

Y

E

S

N

V

C

L

R

R

R

A

E

A

I

V

V

Q

-

S

-

E

-

L

-

F

-

Q

-

D

-

L

-

L

L

K

K

I

L

G

E

Q

E

V

-

M

M

D

G

F

F

P

E

I

I

E
|
E

G

A

L

S

H

H

T

H

E
|
E

A

V

G

A

K

P

G

G

A

Q

M

H

E
|
E

A

I

A
x
D

L

F

S

K

L
x
Y

D

A

T

D

G

A

I

V

G

K

S

A

A

A

D

D

K

Q

A

I

V

Q

L

T

F

F

K

K

T

L

F

V

S

V

K

K

A

T

V

I

A

A

Q

R

Q

Q

R

H

D

G

I

L

T

H

L

A

S

T

F

F

M

M

A

P

K

K

Y

P

S

L

M

F

E

G

M

V

P

N

G
|
G

C

S

G

G

G

M

H
|
H

I

C

H
x
N

L

Q

S
|
S

L

L

K

F

D

-

K

K

D

D

G

N

K

E

P

N

V

V

F

F

F

Y

D

D

A

E

A

T

Q

D

A

E

H

L

Q

G

M

L

S

S

K

Q

T

T

M

A

R

R

H

H

F

Y

I

M

A

A

G

G

Q

I

Q

L

L

K

L

H

M

A

P

R

E

A

F

M

L

A

A

A

M

I

V

T

A

N

P

P

T

T

V

V

N

N

A

S

Y

Y

S

K

R
|
R

L

L

V

V

P

P

G

G

Y

Y

W
x
E

A

A

P

P

T

C

S

Y

A

V

T

A

W

W

G

S

V

A

D

S

N

N

R
|
R

T

S

C

P

A

M

L

I

R
|
R

V

I

V

P

G

A

G

S

S

R

P

G

K

L

S

S

K

T

R
|
R

I

V

E
|
E

Y

V

R
|
R

V

N

T

P

S

D

A

P

D

A

T

A

N

N

P

P

Y

Y

L

L

V

A

Q

L

A

A

A

V

V

M

L

L

A

R

S

A

G

G

L

L

W

D

G

G

I

I

E

K

H

R

E

Q

L

M

E

A

P

L

T

P

P

A

P

P

S

I

T

D

G

R

N

N

A

I

Y

Y

V

V

Q

M

K

S

-

E

-

R

-

I

V

E

S

E

G

G

E

I

L

P

T

S

L

L

P

P

R

A

T

D

L

L

A

K

E

E

A

A

A

L

G

S

R

E

L

L

R

I

S

R

S

S

R

E

A

V

A

I

R

S

D

D

C

A

F

L

G

G

D

D

A

H

F

A

V

L

D

A

H

Y

F

F

A

Y

S

E

T

L

R

K

E

E

W

I

E

E

D

W

R

D

E

M

Y

Y

R

R

R

T

H

Q

V

V

S

H

D

Q

W

W

E

E

L

R

K

D

R

Q

Y

Y

F

L

E

T

I

L

binding adenosine-5'-diphosphate: N123 (≠ S142), G125 (= G143), E127 (= E145), E179 (= E203), D193 (≠ A217), Y196 (≠ L220), N242 (≠ H266), S244 (= S268), R316 (= R341), R326 (= R351)
binding magnesium ion: E127 (= E145), E127 (= E145), E129 (= E147), E184 (= E208), E191 (= E215), E191 (= E215), H240 (= H264), E328 (= E353)
binding l-methionine-s-sulfoximine phosphate: E127 (= E145), E129 (= E147), E184 (= E208), E191 (= E215), G236 (= G260), H240 (= H264), R293 (= R318), E299 (≠ W324), R311 (= R336), R330 (= R355)

7tfaB Glutamine synthetase (see paper)
29% identity, 98% coverage: 9:458/459 of query aligns to 3:440/441 of 7tfaB

query
sites
7tfaB

T

T

A

R

A

E

D

D

A

I

R

I

K

R

I

I

V

A

E

E

R

E

G

N

L

V

T

R

H

F

V

I

E

R

V

L

A

Q

V

F

T

T

D

D

L

L

D

L

G

G

V

T

L

I

R

K

G

N

K

V

Y

E

L

I

S

P

R

V

D

S

K

Q

F

L

F

E

S

K

A

A

L

L

E

D

N

N

S

K

F

M

G

M

F

F

C

-

S

-

V

-

L

-

F

-

G

-

W

-

D

-

C

-

N

-

D

-

E

-

L

-

Y

-

D

-

E

D

A

G

C

S

K

S

I

I

E

E

Y

-

T

-

G

-

W

-

H

-

N

-

G

G

Y
|
Y

R

-

D

-

E

-

V

V

R
|
R

V

I

I

E

P

E

E

S

S

D

C

M

R

Y

E

L

L

Y

P

P

H

D

K

L

N

D

S

T

L

W

L

V

F

V

L

F

A

P

E

W

A

V

H

T

S

S

E

D

H

R

G

V

A

A

R

R

-

L

V

I

C

C

-

D

-

I

-

Y

-

K

-

P

-

D

-

G

-

S

-

P

-

F

-

A

-

G

-

D

P

P

R

R

N

G

L

I

L

L

S

K

R

R

V

V

L

L

A

K

R

E

A

A

D

E

E

E

L

L

G

G

F

Y

S

T

V

S

V

M

S

N

-

V

G

G

F

P

E
|
E

Y

P

E
|
E

F

F

Y

F

G

L

F

F

-

K

-

T

-

D

-

E

-

K

-

G

N

D

E

P

T

T

P

T

Q

E

S

L

A

N

A

D

E

Q

K

G

G

G

Y
|
Y

R

F

D

D

L

L

K

-

L

-

T

A

P

P

T

M

S

D

C

L

G

G

Y

E

S

N

V

C

L

R

R

R

A

E

A

I

V

V

Q

-

S

-

E

-

L

-

F

-

Q

-

D

-

L

-

L

L

K

K

I

L

G

E

Q

E

V

-

M

M

D

G

F

F

P

E

I

I

E

E

G

A

L

S

H

H

T

H

E
|
E

A
x
V

G

A

K

P

G

G

A

Q

M

H

E
|
E

A

I

A

D

L

F

S

K

L

Y

D

A

T

D

G

A

I

V

G

K

S

A

A

A

D

D

K

Q

A

I

V

Q

L

T

F

F

K

K

T

L

F

V

S

V

K

K

A

T

V

I

A

A

Q

R

Q

Q

R

H

D

G

I

L

T

H

L

A

S

T

F

F

M

M

A

P

K

K

Y

P

S

L

M

F

E

G

M

V

P
x
N

G
|
G

C

S

G

G

G

M

H
|
H

I

C

H

N

L

Q

S

S

L

L

K

F

D

-

K

K

D

D

G

N

K

E

P

N

V

V

F

F

F

Y

D

D

A

E

A

T

Q

D

A

E

H

L

Q

G

M

L

S

S

K

Q

T

T

M

A

R

R

H

H

F

Y

I

M

A

A

G

G

Q

I

Q

L

L

K

L

H

M

A

P

R

E

A

F

M

L

A

A

A

M

I

V

T

A

N

P

P

T

T

V

V

N

N

A

S

Y

Y

S

K

R
|
R

L

L

V

V

P

P

G
|
G

Y
|
Y

W
x
E

A

A

P

P

T

C

S

Y

A

V

T

A

W

W

G

S

V

A

D

S

N

N

R
|
R

T

S

C

P

A

M

L

I

R

R

V

I

V

P

G

A

G

S

S

R

P

G

K

L

S

S

K

T

R

R

I

V

E
|
E

Y

V

R

R

V

N

T

P

S

D

A

P

D

A

T

A

N

N

P

P

Y

Y

L

L

V

A

Q

L

A

A

A

V

V

M

L

L

A

R

S

A

G

G

L

L

W

D

G

G

I

I

E

K

H

R

E

Q

L

M

E

A

P

L

T

P

P

A

P

P

S

I

T

D

G

R

N

N

A

I

Y

Y

V

V

Q

M

K

S

-

E

-

R

-

I

V

E

S

E

G

G

E

I

L

P

T

S

L

L

P

P

R

A

T

D

L

L

A

K

E

E

A

A

A

L

G

S

R

E

L

L

R

I

S

R

S

S

R

E

A

V

A

I

R

S

D

D

C

A

F

L

G

G

D

D

A

H

F

A

V

L

D

A

H

Y

F

F

A

Y

S

E

T

L

R

K

E

E

W

I

E

E

D

W

R

D

E
x
M

Y

Y

R

R

R

T

H

Q

V

V

S

H

D

Q

W

W

E

E

L

R

K

D

R

Q

Y

Y

F

L

E

T

I

L

binding glutamine: E131 (= E147), Y153 (= Y163), E186 (= E208), G238 (= G260), H242 (= H264), R295 (= R318), E301 (≠ W324)
binding magnesium ion: E129 (= E145), E131 (= E147), E186 (= E208), E193 (= E215), H242 (= H264), E330 (= E353)
binding : Y58 (= Y85), R60 (= R91), V187 (≠ A209), N237 (≠ P259), G299 (= G322), Y300 (= Y323), R313 (= R336), M424 (≠ E442)

8oozA Glutamine synthetase (see paper)
28% identity, 97% coverage: 15:457/459 of query aligns to 8:428/430 of 8oozA

query
sites
8oozA

K

R

I

I

V

V

E

E

R

K

G

N

L

V

T

R

H

F

V

V

E

R

V

L

A

Q

V

F

T

V

D

D

L

V

D

Q

G

G

V

I

L

P

R

K

G

N

K

V

Y

A

L

I

S

P

R

V

D

G

K

Q

F

L

F

E

S

K

A

A

L

L

E

G

N

P

S

G

F

I

G

H

F

F

C

-

S

-

V

-

L

-

F

-

G

-

W

-

D

-

C

-

N

-

D

-

E

-

L

-

Y

-

D

-

E

D

A

G

C

S

K

S

I

I

E

E

Y

G

T

S

G

D

W

M

H

V

N

L

G

R

Y

P

R

D

D

P

D

D

E

T

V

F

R

R

V

V

I

L

P

P

E

W

S

S

C

T

R

A

E

E

L

A

P

-

H

-

K

-

N

-

S

R

L

L

L

I

F

C

L

D

A

I

E

E

A

L

H

P

S

D

E

G

H

K

G

P

A

F

R

M

V

G

C

C

P

P

R

R

N

Q

L

V

L

L

S

K

R

K

V

N

L

M

A

E

R

E

A

A

D

A

E

K

L

L

G

G

F

Y

S

V

V

M

V

N

S

T

G
|
G

F

P

E
|
E

Y

M

E

E

F

F

Y

F

G

L

F

F

-

K

-

R

-

Q

-

D

-

G

-

M

-

P

N

T

E

N

T

I

P

P

Q

Q

S

D

A

R

A

G

E

-

K

-

G

G

Y

Y

R

F

D

D

L

L

K

A

L

P

L

I

T

D

P

-

T

-

S

-

C

-

G

-

Y

-

S

-

V

-

L

-

R

-

A

-

V

L

Q

A

S

E

E

E

L

I

F

K

Q

R

D

E

L

I

L

V

K

L

I

V

G

L

Q

E

V

E

M

M

D

G

F

F

P

E

I

V

E
|
E

G

A

L

A

H

H

T

H

E

E

A

V

G

A

K

F

G

G

A

Q

M

H

E

E

A

I

A

D

L
x
F

S
x
K

L
x
Y

D

D

T

N

G

A

I

L

G

A

S

T

A

A

D

D

K

N

A

V

V

I

L

T

F

L

K

K

T

Y

F

V

S

A

K

K

A

T

V

L

A

A

Q

L

Q

Q

R

H

D

G

I

L

T

H

L

A

S

T

F

F

M

M

A

P

K

K

Y

P

S

I

M

F

E

G

M

V

P

N

G

G

C

S

G

G

G

M

H
|
H

I

T

H
x
N

L

T

S
|
S

L

L

K

F

D

-

K

K

D

D

G

G

K

K

P

N

V

A

F

F

F

Y

D

D

A

P

A

D

Q

A

A

P

H

D

Q

Q

M

I

S

S

K

D

T

T

M

L

R

R

H

Y

F

F

I

V

A

G

G

G

Q

V

Q

L

L

K

L

H

M

I

P

R

E

A

F

I

L

T

A

A

M

I

V

T

A

N

P

P

T

L

V

V

N

N

A

S

Y

Y

S

K

R

R

L

L

V

V

P

P

G

G

Y

Y

W

E

A

A

P

P

T

V

S

Y

A

I

T

T

W

W

G

S

V

G

D

P

N

N

R

R

T

S

C

S

A

L

L

I

R

R

V

V

V

P

G

A

G

P

S

R

P

G

K

N

S
|
S

K

T

R
|
R

I

I

E
|
E

Y

I

R

R

V

S

T

P

S

D

A

P

D

S

T

C

N

N

P

P

Y

Y

L

L

V

A

Q

F

A

A

V

I

L

L

A

A

S

A

G

G

L

L

W

D

G

G

I

V

E

K

H

N

E

K

L

I

E

E

P

P

T

P

P

E

P

R

S

V

T

E

G

K

N

N

A

I

Y

Y

V

K

-

L

-

T

-

E

Q

R

K

E

V

K

S

L

G

G

E

I

L

G

T

M

L

L

P

P

R

G

T

T

L

L

A

K

E

E

A

A

A

I

G

E

R

C

L

F

R

K

S

E

S

D

R

E

A

L

R

V

D

S

C

A

F

L

G

G

D

E

A

H

F

V

V

S

D

Q

H

S

F

I

A

I

S

N

T

V

R

A

E

M

W

A

E

D

D

W

R

D

E

S

Y

Y

R

R

R

T

H

Q

V

V

S

H

D

Q

W

W

E

E

L

L

K

D

R

R

Y

Y

F

L

E

Q

binding adenosine-5'-triphosphate: G117 (= G143), E170 (= E203), F185 (≠ L218), K186 (≠ S219), Y187 (≠ L220), N233 (≠ H266), S235 (= S268), S315 (= S349), R317 (= R351)
binding magnesium ion: E119 (= E145), H231 (= H264), E319 (= E353)

7tenA Glutamine synthetase (see paper)
32% identity, 73% coverage: 122:458/459 of query aligns to 106:441/442 of 7tenA

query
sites
7tenA

P

P

R

R

N

A

L

N

L

L

S

K

R

R

V

V

L

L

A

K

R

E

A

M

D

E

E

E

L

L

G

G

F

F

S

T

V

E

V

F

S

N

-

L

G
|
G

F

P

E
|
E

Y

P

E
|
E

F

F

Y

F

G

L

F

F

-

K

-

L

-

D

-

E

N

N

E

R

T

R

P

P

-

T

-

L

Q

E

S

L

A

N

A

D

E

S

K

G

G

G

Y

Y

R

F

D

D

L

L

K

-

L

-

T

A

P

P

T

T

S

D

C

L

G

G

Y

E

S

N

V

C

L

R

R

R

A

-

V

-

Q

-

S

-

E

-

L

-

F

-

Q

-

D

D

L

I

L

V

K

L

I

E

G

L

Q

E

V

E

M

M

D

G

F

F

P

E

I

I

E
|
E

G

A

L

S

H

H

T

H

E
|
E

A

V

G

A

K

P

G

G

A

Q

M

H

E
|
E

A

I

A
x
D

L
x
F

S
x
K

L
x
Y

D

E

T

D

G

A

I

I

G

T

S

A

A

C

D

D

K

S

A

I

V

Q

L

T

F

F

K

K

T

L

F

V

S

V

K

K

A

T

V

I

A

A

Q

R

Q

K

R

H

D

G

I

L

T

H

L

A

S

T

F

F

M

M

A

P

K

K

Y

P

S

L

M

F

E

G

M

V

P
x
N

G
|
G

C

S

G

G

G

M

H
|
H

I

F

H
x
N

L

M

S
|
S

L

L

K

F

D

N

K

E

D

K

G

G

K

N

P

-

V

A

F

F

D

D

A

E

A

S

Q

G

A

E

H

L

Q

E

M

L

S

S

K

Q

T

T

M

A

R

Y

H

H

F

F

I

L

A

A

G

G

Q

M

Q

L

L

K

L

H

M

A

P

R

E

G

F

Y

L

T

A

A

M

V

V

T

A

N

P

P

T

T

V

I

N

N

A

S

Y

F

S

K

R
|
R

L

L

V

V

P

P

G

G

Y

Y

W
x
E

A

A

P

P

T

C

S

Y

A

I

T

A

W

W

G

S

V

G

D

K

N

N

R
|
R

T

S

C

P

A

L

L

V

R
|
R

V

V

V

P

G

S

S

R

P

G

K

L

S
|
S

K

T

R
|
R

I

L

E

E

Y

L

R
|
R

V

S

T

V

S

D

A

P

D

S

T

A

N

N

P

P

Y

Y

L

L

V

A

Q

M

A

A

A

V

V

L

L

L

A

K

S

A

G

G

L

L

W

S

G

G

I

I

E

K

H

D

E

E

L

L

E

T

P

P

T

P

P

A

P

P

S

V

T

D

G

R

N

N

A

I

Y

Y

-

G

-

M

-

N

-

E

V

E

Q

R

K

E

V

A

S

T

G

G

E

I

L

Y

T

D

L

L

P

P

R

E

T

S

L

L

A

G

E

H

A

A

A

L

G

I

R

E

L

L

R

E

S

K

S

N

R

E

A

I

R

K

D

D

C

G

F

L

G

G

D

E

A

H

F

I

V

F

D

E

H

H

F

F

A

I

S

E

T

A

R

K

E

T

W

I

E

E

D

C

R

D

E

M

Y

F

R

R

R

T

H

A

V

V

S

H

D

P

W

W

E

E

L

R

K

E

R

Q

Y

Y

F

L

E

E

I

I

binding adenosine-5'-diphosphate: G128 (= G143), E130 (= E145), E182 (= E203), D196 (≠ A217), F197 (≠ L218), K198 (≠ S219), Y199 (≠ L220), N245 (≠ H266), S247 (= S268), R319 (= R341), S327 (= S349), R329 (= R351)
binding l-methionine-s-sulfoximine phosphate: E130 (= E145), E132 (= E147), E187 (= E208), E194 (= E215), N238 (≠ P259), G239 (= G260), H243 (= H264), R296 (= R318), E302 (≠ W324), R314 (= R336), R333 (= R355)

7tf9S L. Monocytogenes gs(14)-q-glnr peptide (see paper)
32% identity, 73% coverage: 122:458/459 of query aligns to 107:442/443 of 7tf9S

query
sites
7tf9S

P

P

R

R

N

A

L

N

L

L

S

K

R

R

V

V

L

L

A

K

R

E

A

M

D

E

E

E

L

L

G

G

F

F

S

T

V

E

V

F

S

N

-

L

G

G

F

P

E
|
E

Y

P

E
|
E

F

F

Y

F

G

L

F

F

-

K

-

L

-

D

-

E

N

N

E

R

T

R

P

P

-

T

-

L

Q

E

S

L

A

N

A

D

E

S

K

G

G

G

Y
|
Y

R

F

D

D

L

L

K

-

L

-

T

A

P

P

T

T

S

D

C

L

G

G

Y

E

S

N

V

C

L

R

R

R

A

-

V

-

Q

-

S

-

E

-

L

-

F

-

Q

-

D

D

L

I

L

V

K

L

I

E

G

L

Q

E

V

E

M

M

D

G

F

F

P

E

I

I

E

E

G

A

L

S

H

H

T

H

E
|
E

A

V

G

A

K

P

G

G

A

Q

M

H

E
|
E

A

I

A

D

L

F

S

K

L

Y

D

E

T

D

G

A

I

I

G

T

S

A

A

C

D

D

K

S

A

I

V

Q

L

T

F

F

K

K

T

L

F

V

S

V

K

K

A

T

V

I

A

A

Q

R

Q

K

R

H

D

G

I

L

T

H

L

A

S

T

F

F

M

M

A

P

K

K

Y

P

S

L

M

F

E

G

M

V

P

N

G
|
G

C

S

G
|
G

G

M

H
|
H

I

F

H

N

L

M

S

S

L

L

K

F

D

N

K

E

D

K

G

G

K

N

P

-

V

A

F

F

D

D

A

E

A

S

Q

G

A

E

H

L

Q

E

M

L

S

S

K

Q

T

T

M

A

R

Y

H

H

F

F

I

L

A

A

G

G

Q

M

Q

L

L

K

L

H

M

A

P

R

E

G

F

Y

L

T

A

A

M

V

V

T

A

N

P

P

T

T

V

I

N

N

A

S

Y

F

S

K

R
|
R

L

L

V

V

P

P

G

G

Y

Y

W
x
E

A

A

P

P

T

C

S

Y

A

I

T

A

W

W

G

S

V

G

D

K

N

N

R

R

T

S

C

P

A

L

L

V

R

R

V

V

V

P

G

S

S

R

P

G

K

L

S

S

K

T

R

R

I

L

E
|
E

Y

L

R

R

V

S

T

V

S

D

A

P

D

S

T

A

N

N

P

P

Y

Y

L

L

V

A

Q

M

A

A

A

V

V

L

L

L

A

K

S

A

G

G

L

L

W

S

G

G

I

I

E

K

H

D

E

E

L

L

E

T

P

P

T

P

P

A

P

P

S

V

T

D

G

R

N

N

A

I

Y

Y

-

G

-

M

-

N

-

E

V

E

Q

R

K

E

V

A

S

T

G

G

E

I

L

Y

T

D

L

L

P

P

R

E

T

S

L

L

A

G

E

H

A

A

A

L

G

I

R

E

L

L

R

E

S

K

S

N

R

E

A

I

R

K

D

D

C

G

F

L

G

G

D

E

A

H

F

I

V

F

D

E

H

H

F

F

A

I

S
x
E

T

A

R

K

E

T

W
x
I

E

E

D

C

R

D

E
x
M

Y

F

R

R

R

T

H

A

V

V

S

H

D

P

W

W

E

E

L

R

K

E

R

Q

Y

Y

F

L

E

E

I

I

binding glutamine: E133 (= E147), Y155 (= Y163), E188 (= E208), G240 (= G260), G242 (= G262), R297 (= R318), E303 (≠ W324)
binding magnesium ion: E131 (= E145), E133 (= E147), E188 (= E208), E195 (= E215), H244 (= H264), E332 (= E353)
binding : E418 (≠ S434), I422 (≠ W438), M426 (≠ E442)

Sites not aligning to the query:

binding : 59, 60

8ooxB Glutamine synthetase (see paper)
28% identity, 97% coverage: 15:457/459 of query aligns to 8:436/438 of 8ooxB

query
sites
8ooxB

K

R

I

I

V

V

E

E

R

K

G

N

L

V

T

R

H

F

V

V

E

R

V

L

A

Q

V

F

T

V

D

D

L

V

D

Q

G

G

V

I

L

P

R

K

G

N

K

V

Y

A

L

I

S

P

R

V

D

G

K

Q

F

L

F

E

S

K

A

A

L

L

E

G

N

P

S

G

F

I

G

H

F

F

-

D

C

G

S

S

V

S

L

I

F

E

G

G

W

F

D

-

C

-

N

-

D

-

E

-

L

-

Y

-

D

-

E

-

A

-

C

V

K

R

I

I

E

E

Y

E

T

S

G

-

W

-

H

-

N

-

G

-

Y

-

R

-

D

D

D

M

E

V

V

L

R

R

V

P

I

D

P

P

E

D

S

T

C

F

R

R

E

V

L

L

P

P

H

W

K

S

N

G

S

N

-

T

-

A

-

E

-

A

-

R

L

L

L

I

F

C

L

D

A

I

E

E

A

L

H

P

S

D

E

G

H

K

G

P

A

F

R

M

V

G

C

C

P

P

R

R

N

Q

L

V

L

L

S

K

R

K

V

N

L

M

A

E

R

E

A

A

D

A

E

K

L

L

G

G

F

Y

S

V

V

M

V

N

S

T

G

G

F

P

E

E

Y

M

E
|
E

F

F

Y

F

G

L

F

F

-

K

-

R

-

Q

-

D

-

G

-

M

-

P

N

T

E

N

T

I

P

P

Q

Q

S

D

A

R

A

G

E

-

K

-

G

G

Y

Y

R

F

D

D

L

L

K

A

L

P

L

I

T

D

P

-

T

-

S

-

C

-

G

-

Y

-

S

-

V

-

L

-

R

-

A

-

V

L

Q

A

S

E

E

E

L

I

F

K

Q

R

D

E

L

I

L

V

K

L

I

V

G

L

Q

E

V

E

M

M

D

G

F

F

P

E

I

V

E

E

G

A

L

A

H

H

T

H

E
|
E

A

V

G

A

K

F

G

G

A

Q

M

H

E
|
E

A

I

A

D

L

F

S

K

L

Y

D

D

T

N

G

A

I

L

G

A

S

T

A

A

D

D

K

N

A

V

V

I

L

T

F

L

K

K

T

Y

F

V

S

A

K

K

A

T

V

L

A

A

Q

L

Q

Q

R

H

D

G

I

L

T

H

L

A

S

T

F

F

M

M

A

P

K

K

Y

P

S

I

M

F

E

G

M

V

P

N

G

G

C

S

G

G

G

M

H

H

I

T

H

N

L

T

S

S

L

L

K

F

D

-

K

K

D

D

G

G

K

K

P

N

V

A

F

F

F

Y

D

D

A

P

A

D

Q

A

A

P

H

D

Q

Q

M

I

S

S

K

D

T

T

M

L

R

R

H

Y

F

F

I

V

A

G

G

G

Q

V

Q

L

L

K

L

H

M

I

P

R

E

A

F

I

L

T

A

A

M

I

V

T

A

N

P

P

T

L

V

V

N

N

A

S

Y

Y

S

K

R

R

L

L

V

V

P

P

G

G

Y

Y

W

E

A

A

P

P

T

V

S

Y

A

I

T

T

W

W

G

S

V

G

D

P

N

N

R

R

T

S

C

S

A

L

L

I

R

R

V

V

V

P

G

A

G

P

S

R

P

G

K

N

S

S

K

T

R

R

I

I

E

E

Y

I

R

R

V

S

T

P

S

D

A

P

D

S

T

C

N

N

P

P

Y

Y

L

L

V

A

Q

F

A

A

V

I

L

L

A

A

S

A

G

G

L

L

W

D

G

G

I

V

E

K

H

N

E

K

L

I

E

E

P

P

T

P

P

E

P

R

S

V

T

E

G

K

N

N

A

I

Y

Y

V

K

-

L

-

T

-

E

Q

R

K

E

V

K

S

L

G

G

E

I

L

G

T

M

L

L

P

P

R

G

T

T

L

L

A

K

E

E

A

A

A

I

G

E

R

C

L

F

R

K

S

E

S

D

R

E

A

L

R

V

D

S

C

A

F

L

G

G

D

E

A

H

F

V

V

S

D

Q

H

S

F

I

A

I

S

N

T

V

R

A

E

M

W

A

E

D

D

W

R

D

E

S

Y

Y

R

R

R

T

H

Q

V

V

S

H

D

Q

W

W

E

E

L

L

K

D

R

R

Y

Y

F

L

E

Q

binding magnesium ion: E129 (= E147), E183 (= E208), E190 (= E215)

8oooA Glutamine synthetase from methanothermococcus thermolithotrophicus in complex with 2-oxoglutarate and mgatp at 2.15 a resolution (see paper)
29% identity, 73% coverage: 122:458/459 of query aligns to 112:447/447 of 8oooA

query
sites
8oooA

P

P

R

R

N

G

L

C

L

L

S

K

R

R

V

V

L

M

A

E

R

E

-

F

A

K

D

K

E

E

L

F

G

N

F

G

S

E

V

Y

V

F

S

V

G

G

F

P

E
|
E

Y

P

E

E

F

F

Y

F

G

L

F

L

N

K

E

K

T

D

P

P

Q

H

S

N

-

P

-

H

-

K

-

Y

-

I

-

P

A

A

A

D

E

D

K

G

G

G

Y

Y

R

F

D

D

L

L

K

E

L

P

L

M

T

D

P

E

T

A

S
x
P

C

-

G

-

Y

-

S

-

V

-

L

-

R

-

A

-

V

-

Q

-

S

-

E

D

L

I

F
x
R

Q
x
R

D

D

L

I

L

V

K

F

I

A

G

L

Q

E

V

N

M

L

D

G

F

F

P

H

I

V

E
|
E

G

A

L
x
S

H

H

T

H

E

E

A

V

G

A

K

P

G

G

A

Q

M

H

E

E

A

V

A

D

L
x
F

S
x
K

L
x
F

D

D

T

D

G

A

I

L

G

K

S

T

A

A

D

D

K

S

A

V

V

I

L

T

F

F

K

K

T

T

F

T

S

I

K

K

A

T

V

I

A

A

Q

E

Q

Q

R

H

D

G

I

L

T

K

L

A

S

T

F

F

M

M

A

P

K

K

Y

P

S

F

M

F

E

G

M

M

P

N

G

G

C

S

G

G

G

M

H

H

I

C

H
|
H

L

Q

S
|
S

L

I

K

W

D

-

K

L

D

N

G

G

K

E

P

P

V

S

F

F

F

Y

D

D

A

E

A

N

Q

A

A

P

H

Y

Q

Q

M

L

S

S

K

E

T

T

M

C

R

M

H

N

F

Y

I

V

A

A

G

G

Q

I

Q

L

L

K

L

H

M

A

P

K

E

A

F

I

L

V

A

A

M

I

V

T

A

N

P

P

T

T

V

V

N

N

A

S

Y

Y

S

K

R

R

L

L

V

V

P

P

G

G

Y

Y

W

E

A

A

P

P

T

V

S

N

A

I

T

A

W

W

G

A

V

N

D

S

N

N

R

R

T

S

C

A

A

I

L

I

R
|
R

V

V

V

P

G

A

S

R

P

G

K

K

S

G

K

T

R
|
R

I

I

E

E

Y

F

R

R

V

A

T

P

S

D

A

P

D

S

T

C

N

N

P

P

Y

Y

L

L

V

A

Q

F

A

T

A

V

V

M

L

L

A

A

S

A

G

G

L

L

W

D

G

G

I

V

E

K

H

N

E

K

L

L

E

D

P

A

T

P

P

E

P

P

S

V

T

E

G

R

N

N

A

I

Y

F

V

A

-

M

-

S

-

E

-

A

-

E

Q

K

K

K

V

E

S

L

G

G

E

I

L

E

T

S

L

V

P

P

R

A

T

N

L

L

A

K

E

A

A

A

A

L

G

D

R

E

L

L

R

E

S

N

R

D

A

V

A

L

R

K

D

N

C

A

F

L

G

G

D

K

A

H

F

I

V

F

D

E

H

S

F

F

A

L

S

E

T

I

R

K

E

N

W

A

E

E

D

W

R

D

E

S

Y

F

R

R

R

T

H

S

V

V

S

T

D

D

W

W

E

E

L

T

K

T

R

A

Y

Y

F

L

E

K

I

I

binding 2-oxoglutaric acid: P170 (≠ S173), R173 (≠ F188), R174 (≠ Q189), S190 (≠ L205)
binding adenosine-5'-triphosphate: E136 (= E145), E188 (= E203), F203 (≠ L218), K204 (≠ S219), F205 (≠ L220), H251 (= H266), S253 (= S268), R325 (= R341), R335 (= R351)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 17, 19, 33, 87, 93

8ooqB Glutamine synthetase from Methanothermococcus thermolithotrophicus (see paper)
29% identity, 73% coverage: 122:458/459 of query aligns to 111:446/446 of 8ooqB

query
sites
8ooqB

P

P

R

R

N

G

L

C

L

L

S

K

R

R

V

V

L

M

A

E

R

E

-

F

A

K

D

K

E

E

L

F

G

N

F

G

S

E

V

Y

V

F

S

V

G

G

F

P

E

E

Y

P

E
|
E

F

F

Y

F

G

L

F

L

N

K

E

K

T

D

P

P

Q

H

S

N

-

P

-

H

-

K

-

Y

-

I

-

P

A

A

A

D

E

D

K

G

G

G

Y

Y

R

F

D

D

L

L

K

E

L

P

L

M

T

D

P

E

T

A

S
x
P

C

-

G

-

Y

-

S

-

V

-

L

-

R

-

A

-

V

-

Q

-

S

-

E

D

L

I

F
x
R

Q
x
R

D

D

L

I

L

V

K

F

I

A

G

L

Q

E

V

N

M

L

D

G

F

F

P

H

I

V

E

E

G

A

L
x
S

H

H

T

H

E
|
E

A

V

G

A

K

P

G

G

A

Q

M

H

E
|
E

A

V

A

D

L

F

S

K

L

F

D

D

T

D

G

A

I

L

G

K

S

T

A

A

D

D

K

S

A

V

V

I

L

T

F

F

K

K

T

T

F

T

S

I

K

K

A

T

V

I

A

A

Q

E

Q

Q

R

H

D

G

I

L

T

K

L

A

S

T

F

F

M

M

A

P

K

K

Y

P

S

F

M

F

E

G

M

M

P

N

G

G

C

S

G

G

G

M

H

H

I

C

H

H

L

Q

S

S

L

I

K

W

D

-

K

L

D

N

G

G

K

E

P

P

V

S

F

F

F

Y

D

D

A

E

A

N

Q

A

A

P

H

Y

Q

Q

M

L

S

S

K

E

T

T

M

C

R

M

H

N

F

Y

I

V

A

A

G

G

Q

I

Q

L

L

K

L

H

M

A

P

K

E

A

F

I

L

V

A

A

M

I

V

T

A

N

P

P

T

T

V

V

N

N

A

S

Y

Y

S

K

R

R

L

L

V

V

P

P

G

G

Y

Y

W

E

A

A

P

P

T

V

S

N

A

I

T

A

W

W

G

A

V

N

D

S

N

N

R

R

T

S

C

A

A

I

L

I

R

R

V

V

V

P

G

A

S

R

P

G

K

K

S

G

K

T

R

R

I

I

E

E

Y

F

R

R

V

A

T

P

S

D

A

P

D

S

T

C

N

N

P

P

Y

Y

L

L

V

A

Q

F

A

T

A

V

V

M

L

L

A

A

S

A

G

G

L

L

W

D

G

G

I

V

E

K

H

N

E

K

L

L

E

D

P

A

T

P

P

E

P

P

S

V

T

E

G

R

N

N

A

I

Y

F

V

A

-

M

-

S

-

E

-

A

-

E

Q

K

K

K

V

E

S

L

G

G

E

I

L

E

T

S

L

V

P

P

R

A

T

N

L

L

A

K

E

A

A

A

A

L

G

D

R

E

L

L

R

E

S

N

R

D

A

V

A

L

R

K

D

N

C

A

F

L

G

G

D

K

A

H

F

I

V

F

D

E

H

S

F

F

A

L

S

E

T

I

R

K

E

N

W

A

E

E

D

W

R

D

E

S

Y

F

R

R

R

T

H

S

V

V

S

T

D

D

W

W

E

E

L

T

K

T

R

A

Y

Y

F

L

E

K

I

I

binding 2-oxoglutaric acid: P169 (≠ S173), R172 (≠ F188), R173 (≠ Q189), S189 (≠ L205)
binding magnesium ion: E137 (= E147), E192 (= E208), E199 (= E215)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 16, 18, 32, 86, 92

5dm3A Crystal structure of glutamine synthetase from chromohalobacter salexigens dsm 3043(csal_0679, target efi-550015) with bound adp
32% identity, 73% coverage: 122:457/459 of query aligns to 84:374/374 of 5dm3A

query
sites
5dm3A

P

P

R

R

N

A

L

I

L

L

S

K

R

R

V

Q

L

L

A

A

R

R

A

L

D

E

E

A

L

M

G

G

F

L

S

E

V

A

V
x
I

S

M

G

A

F

T

E
|
E

Y

L

E
|
E

F

F

Y

F

G

L

F

F

N

E

E

K

T

S

P

L

Q

D

S

T

A

T

A

K

E

E

K

E

G

-

Y

-

R

-

D

-

L

-

K

-

L

-

T

-

P

-

T

-

S

-

C

-

G

-

Y

-

S

H

V

V

L

L

R

R

A

P

A

-

V

-

Q

-

S

-

E

-

L

L

F

R

Q

N

D

H

L

L

L

H

K

A

I

A

G

G

Q

-

V

-

M

-

D

-

F

I

P

P

I

V

E
|
E

G

G

L

-

H

-

T

-

E

T

A

K

G

G

K
x
E

G

G

A

Q

M

E

E
|
E

A

L

A

N

L

I

S
x
R

L
x
C

D

A

T

K

G

A

I

L

G

D

S

T

A

A

D

D

K

Y

A

H

V

T

L

I

F

A

K

K

T

H

F

A

S

T

K

K

A

E

V

I

A

A

Q

W

Q

Q

R

Q

D

G

I

R

T

A

L

V

S

T

F

F

M

L

A

S

K

K

Y

W

S

H

M

H

E

A

M

H

P

A

G

G

C

S

G

S

H
|
H

I

I

H
|
H

L

Q

S
|
S

L

L

K

W

D

-

K

K

D

Q

G

G

K

L

P

P

V

A

F

F

F

H

D

D

A

E

A

R

Q

D

A

A

H

L

Q

G

M

M

S

S

K

A

T

L

M

M

R

K

H

H

F

Y

I

L

A

A

G

G

Q

L

L

K

L

Y

M

A

P

P

E

D

F

Y

L

T

A

Y

M

F

V

L

A

A

P

P

T

Y

V

L

N

N

A

S

Y

Y

S

K

R

R

L

-

V

-

P

-

G

-

Y

-

W

F

A

Q

P

P

T

T

S

R

A

T

T

V

W

W

G

S

V

V

D

D

N

N

R
|
R

T

T

C

A

A

G

L

F

R

R

V

L

V

C

G

A

G

E

S

G

P

T

K

R

S

A

K

V

R
|
R

I

I

E
|
E

Y

C

R
|
R

V

I

T

G

S

G

A

S

D

D

T

L

N

N

P

P

Y

Y

L

L

V

A

Q

M

A

A

A

G

V

Q

L

L

A

A

S

A

G

G

L

I

W

K

G

G

I

I

E

E

H

E

E

C

L

L

E

A

P

L

T

P

P

P

S

A

T

G

G

-

N

-

A

-

Y

-

V

-

Q

-

K

-

V

-

S

-

G

-

E

-

L

-

T

L

L

I

P

P

R

Q

T

N

L

L

A

R

E

D

A

A

A

M

G

E

R

A

L

L

R

R

S

G

S

S

R

T

A

M

A

L

R

R

D

E

C

A

F

M

G

G

D

E

A

D

F

V

V

V

D

D

H

H

F

Y

A

V

S

R

T

A

R

A

E

E

W

V

E

E

D

L

R

E

E

D

Y

F

R

Q

R

R

H

V

V

V

S

S

D

D

W

Y

E

E

L

V

K

A

R

R

Y

G

F

F

E

E

active site: E107 (= E145), E109 (= E147), E146 (≠ K211), E150 (= E215), H199 (= H264), R265 (= R336), E282 (= E353), R284 (= R355)
binding adenosine-5'-diphosphate: I103 (≠ V141), E141 (= E203), R154 (≠ S219), C155 (≠ L220), H201 (= H266), S203 (= S268), R280 (= R351)

7tdvC Glutamine synthetase (see paper)
28% identity, 98% coverage: 9:457/459 of query aligns to 4:441/443 of 7tdvC

query
sites
7tdvC

T

T

A

K

A

E

D

D

A

I

R

R

K

K

I

F

V

V

E

E

R

E

G

N

L

V

T

R

H

Y

V

L

E

R

V

L

A

Q

V

F

T

T

D

D

L

I

D

L

G

G

V

T

L

I

R

K

G

N

K

V

Y

E

L

V

S

P

R

V

D

S

K

Q

F

L

F

E

S

K

A

V

L

L

E

D

N

N

S

E

F

M

G

M

F

F

-

D

C

G

S

S

V

S

L

I

F

E

G

G

W

F

D

-

C

-

N

-

D

-

E

-

L

-

Y

-

D

-

E

-

A

-

C

V

K

R

I

I

E

E

Y

E

T

S

G

D

W

M

H

Y

N

L

G

H

Y

P

R

D

D

L

D

D

E

T

V

W

R

V

V

I

I

F

P

P

E

W

S

T

C

A

R

G

E

Q

L

-

P

-

H

-

K

G

N

K

S

V

L

A

L

R

F

L

L

I

A

C

E

D

A

V

H

Y

S

K

E

T

H

D

G

G

A

T

R

P

V

F

-

E

-

G

C

D

P

P

R

R

N

A

L

N

L

L

S

K

R

R

V

V

L

L

A

K

R

E

A

M

D

E

E

D

L

L

G

G

F

F

S

T

V

D

V

F

S

N

-

L

G
|
G

F

P

E
|
E

Y

P

E
|
E

F

F

Y

F

G

L

F

F

N

-

E

-

T

-

P

-

Q

-

S

K

A

L

A

D

E

E

K

K

G

G

Y

E

R

P

D

T

L

L

K

E

L

L

-

N

-

D

-

G

-

Y

-

F

-

D

L

L

T

A

P

P

T

T

S

D

C

L

G

G

Y

E

S

N

V

C

L

R

R

R

A

-

V

-

Q

-

S

-

E

-

L

-

F

-

Q

-

D

D

L

I

L

V

K

L

I

E

G

L

Q

E

V

D

M

M

D

G

F

F

P

D

I

I

E
|
E

G

A

L

S

H

H

T

H

E
|
E

A

V

G

A

K

P

G

G

A

Q

M

H

E
|
E

A

I

A
x
D

L
x
F

S
x
K

L
x
Y

D

A

T

D

G

A

I

V

G

T

S

A

A

C

D

D

K

N

A

I

V

Q

L

T

F

F

K

K

T

L

F

V

S

V

K

K

A

T

V

I

A

A

Q

R

Q

K

R

H

D

N

I

L

T

H

L

A

S

T

F

F

M

M

A

P

K

K

Y

P

S

L

M

F

E

G

M

V

P

N

G
|
G

C

S

G

G

G

M

H
|
H

I

F

H
x
N

L
x
V

S
|
S

L

L

K

F

D

-

K

K

D

G

G

K

K

E

P

N

V

A

F

F

D

D

A

P

A

N

Q

T

A

E

H

M

Q

G

M

L

S

T

K

E

T

T

M

A

R

Y

H

Q

F

F

I

T

A

A

G

G

Q

V

Q

L

L

K

L

N

M

A

P

R

E

G

F

F

L

T

A

A

M

V

V

C

A

N

P

P

T

L

V

V

N

N

A

S

Y

Y

S

K

R
|
R

L

L

V

V

P

P

G

G

Y

Y

W
x
E

A

A

P

P

T

C

S

Y

A

I

T

A

W

W

G

S

V

G

D

K

N

N

R
|
R

T

S

C

P

A

L

L

I

R
|
R

V

V

V

P

G

S

S

R

P

G

K

L

S
|
S

K

T

R
|
R

I

I

E
|
E

Y

V

R

R

V

S

T

V

S

D

A

P

D

A

T

A

N

N

P

P

Y

Y

L

M

V

A

Q

L

A

A

V

I

L

L

A

E

S

A

G

G

L

L

W

D

G

G

I

I

E

K

H

N

E

K

L

L

E

K

P

V

T

P

P

E

P

P

S

V

T

N

G

Q

N

N

A

I

Y

Y

-

E

-

M

-

N

-

R

-

E

V

E

Q

R

K

E

V

A

S

V

G

G

E

I

L

Q

T

D

L

L

P

P

R

S

T

T

L

L

A

Y

E

T

A

A

A

L

G

K

R

A

L

M

R

R

S

E

S

N

R

E

A

V

A

I

R

K

D

K

C

A

F

L

G

G

D

N

A

H

F

I

V

Y

D

N

H

Q

F

F

A

I

S

N

T

S

R

K

E

S

W

I

E

E

D

W

R

D

E

Y

Y

Y

R

R

R

T

H

Q

V

V

S

S

D

E

W

W

E

E

L

R

K

D

R

Q

Y

Y

F

M

E

K

binding adenosine-5'-diphosphate: G129 (= G143), E131 (= E145), E183 (= E203), D197 (≠ A217), F198 (≠ L218), K199 (≠ S219), Y200 (≠ L220), N246 (≠ H266), V247 (≠ L267), S248 (= S268), R320 (= R341), S328 (= S349), R330 (= R351)
binding magnesium ion: E131 (= E145), E131 (= E145), E133 (= E147), E188 (= E208), E195 (= E215), E195 (= E215), H244 (= H264), E332 (= E353)
binding l-methionine-s-sulfoximine phosphate: E131 (= E145), E133 (= E147), E188 (= E208), E195 (= E215), G240 (= G260), H244 (= H264), R297 (= R318), E303 (≠ W324), R315 (= R336)

7tf6A Glutamine synthetase (see paper)
28% identity, 98% coverage: 9:457/459 of query aligns to 3:436/438 of 7tf6A

query
sites
7tf6A

T

T

A

K

A

E

D

D

A

I

R

R

K

K

I

F

V

A

E

E

R

E

G

N

L

V

T

R

H

Y

V

L

E

R

V

L

A

Q

V

F

T

T

D

D

L

I

D

L

G

G

V

T

L

I

R

K

G

N

K

V

Y

E

L

V

S

P

R

V

D

S

K

Q

F

L

F

E

S

K

A

V

L

L

E

D

N

N

S

E

F

M

G

M

F

F

-

D

C

G

S

S

V

S

L

I

F

E

G

G

W
x
F

D

-

C

-

N

-

D

-

E

-

L

-

Y

-

D

-

E

-

A

-

C

V

K
x
R

I

I

E

E

Y

E

T

S

G

D

W

M

H

Y

N

-

G

L

Y

H

R

P

D

D

D

L

E

D

V

T

R

W

V

V

I

I

P

-

E

-

S

-

C

-

R

-

E

-

L

F

P

P

H

W

K

G

N

K

S

V

L

A

L

R

F

L

L

I

A

C

E

D

A

V

H

Y

S

K

E

T

H

D

G

G

A

T

R

P

V

F

-

E

-

G

C

D

P

P

R

R

N

A

L

N

L

L

S

K

R

R

V

V

L

L

A

K

R

E

A

M

D

E

E

D

L

L

G

G

F

F

S

T

V

D

V

F

S

N

-

L

G

G

F

P

E
|
E

Y

P

E
|
E

F

F

Y

F

G

L

F

F

N

-

E

-

T

-

P

-

Q

-

S

K

A

L

A

D

E

E

K

K

G

G

Y

E

R

P

D

T

L

L

K

E

L

L

-

N

-

D

-

G

-

Y

-

F

-

D

L

L

T

A

P

P

T

T

S

D

C

L

G

G

Y

E

S

N

V

C

L

R

R

R

A

-

V

-

Q

-

S

-

E

-

L

-

F

-

Q

-

D

D

L

I

L

V

K

L

I

E

G

L

Q

E

V

D

M

M

D

G

F

F

P

D

I

I

E

E

G

A

L

S

H

H

T

H

E
|
E

A

V

G

A

K

P

G

G

A

Q

M

H

E
|
E

A

I

A

D

L

F

S

K

L

Y

D

A

T

D

G

A

I

V

G

T

S

A

A

C

D

D

K

N

A

I

V

Q

L

T

F

F

K

K

T

L

F

V

S

V

K

K

A

T

V

I

A

A

Q

R

Q

K

R

H

D

N

I

L

T

H

L

A

S

T

F

F

M

M

A

P

K

K

Y

P

S

L

M

F

E
x
G

M

V

P
x
N

G
|
G

C

S

G

G

G

M

H
|
H

I

F

H

N

L

V

S

S

L

L

K

F

D

-

K

K

D

G

G

K

K

E

P

N

V

A

F

F

D

D

A

P

A

N

Q

T

A

E

H

M

Q

G

M

L

S

T

K

E

T

T

M

A

R

Y

H

Q

F

F

I

T

A

A

G

G

Q

V

Q

L

L

K

L

N

M

A

P

R

E

G

F

F

L

T

A

A

M

V

V

C

A

N

P

P

T

L

V

V

N

N

A

S

Y

Y

S

K

R
|
R

L

L

V

V

P

P

G
|
G

Y
|
Y

W
x
E

A

A

P

P

T

C

S

Y

A

I

T

A

W

W

G

S

V

G

D

K

N

N

R
|
R

T

S

C

P

A

L

L

I

R

R

V

V

V

P

G

S

S

R

P

G

K

L

S

S

K

T

R

R

I

I

E
|
E

Y

V

R

R

V

S

T

V

S

D

A

P

D

A

T

A

N

N

P

P

Y

Y

L

M

V

A

Q

L

A

A

V

I

L

L

A

E

S

A

G

G

L

L

W

D

G

G

I

I

E

K

H

N

E

K

L

L

E

K

P

V

T

P

P

E

P

P

S

V

T

N

G

Q

N

N

A

I

Y
|
Y

-

E

-

M

-

N

-

R

-

E

V

E

Q

R

K

E

V

A

S

V

G

G

E

I

L

Q

T

D

L

L

P

P

R

S

T

T

L

L

A

Y

E

T

A

A

A

L

G

K

R

A

L

M

R

R

S

E

S

N

R

E

A

V

A

I

R

K

D

K

C

A

F

L

G

G

D

N

A

H

F

I

V

Y

D

N

H

Q

F

F

A

I

S

N

T

S

R

K

E

S

W

I

E

E

D

W

R

D

E
x
Y

Y

Y

R

R

R

T

H

Q

V

V

S

S

D

E

W

W

E

E

L

R

K

D

R
x
Q

Y

Y

F

M

E

K

binding glutamine: E128 (= E147), E183 (= E208), G235 (= G260), H239 (= H264), R292 (= R318), E298 (≠ W324)
binding magnesium ion: E126 (= E145), E128 (= E147), E183 (= E208), E190 (= E215), H239 (= H264), E327 (= E353)
binding : F58 (≠ W63), R60 (≠ K75), G232 (≠ E257), N234 (≠ P259), G296 (= G322), Y297 (= Y323), R310 (= R336), Y367 (= Y393), Y421 (≠ E442), Q433 (≠ R454)

Sites not aligning to the query:

binding : 437

A0R083 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
29% identity, 97% coverage: 15:458/459 of query aligns to 10:446/446 of A0R083

query
sites
A0R083

K

R

I

T

V

L

E

E

R

R

G

D

L

I

T

R

H

F

V

V

E

R

V

L

A

W

V

F

T

T

D

D

L

V

D

L

G

G

V

Y

L

L

R

K

G

S

K

V

Y

A

L

I

S

A

R

P

D

A

K

E

F

L

F

E

S

G

A

A

L

F

E

E

N

E

S

G

F

I

G

G

F

F

C

-

S

-

V

-

L

-

F

-

G

-

W

-

D

-

C

-

N

-

D

-

E

-

L

-

Y

-

D

-

E

D

A

G

C

S

K

S

I

I

E

E

Y

-

T

-

G

G

W

F

H

A

N

R

G

V

Y

F

R

E

D

S

D

D

E

T

V

V

-

A

R

R

V

P

I

D

P

P

E

S

S

T

C

F

R

Q

E

V

L

L

P

P

H

W

K

K

N

T

S

S

L

-

F

-

L

-

A

-

E

-

A

D

H

G

S

N

E

H

H

Y

G

S

A

A

R

R

V

M

-

F

C

C

-

D

-

I

-

T

-

M

-

P

-

D

-

G

-

S

-

P

-

S

-

W

-

A

-

D

P

S

R

R

N

H

L

V

L

L

S

R

R

R

V

Q

L

L

A

A

R

K

A

A

D

S

E

D

L

L

G

G

F

F

S

T

V

C

V

Y

S

V

G

H

F

P

E

E

Y

I

E

E

F

F

Y

F

-

L

-

K

-

P

G

G

F

P

N

N

E

D

-

G

-

T

T

P

P

P

Q

E

S

P

A

A

A

D

E

N

K

G

G

G

Y

Y

R

F

D

D

L

Q

K

A

L

V

L

-

T

-

P

-

T

H

S

D

C

A

G

A

Y

P

S

N

V

F

L

R

R

R

A

H

A

A

V

I

Q

E

S

A

E

-

L

-

F

-

Q

-

D

-

L

L

L

E

K

Q

I

M

G

G

Q

I

V

S

M

V

D

E

F

F

P

S

I

-

E

-

G

-

L

-

H

H

T

H

E

E

A

G

G

A

K

P

G

G

A

Q

M

Q

E

E

A

I

A

D

L

L

S

R

L

Y

D

A

T

D

G

A

I

L

G

S

S

M

A

A

D

D

K

N

A

V

V

M

L

T

F

F

K

R

T

Y

F

L

S

V

K

K

A

E

V

V

A

A

Q

L

Q

A

R

D

D

G

I

V

T

R

L

A

S

S

F

F

M

M

A

P

K

K

Y

P

S

F

M

A

E

E

M

H

P

P

G

G

C

S

G

A

G

M

H

H

I

T

H

H

L

M

S

S

L

L

K

F

D

E

K

G

D

D

G

T

K

N

P

-

V

A

F

F

F

H

D

S

A

P

A

D

Q

D

A

P

H

L

Q

Q

M

L

S

S

K

D

T

V

M

A

R

K

H

S

F

F

I

I

A

A

G

G

Q

I

Q

L

L

E

L

H

M

A

P

N

E

E

F

I

L

S

A

A

M

V

V

T

A

N

P

Q

T

W

V

V

N

N

A

S

Y

Y

S

K

R

R

L

L

V

V

P

H

G

G

Y

G

W

E

A

A

P

P

T

T

S

A

A

A

T

S

W

W

G

G

V

A

D

A

N

N

R

R

T

S

C

A

A

L

L

V

R

R

V

V

V

P

G

M

G

Y

S

T

P

P

-

H

-

K

-

V

K

S

S

S

K

R

R

R

I

V

E

E

Y

V

R

R

V

S

T

P

S

D

A

S

D

A

T

C

N

N

P

P

Y

Y

L

L

V

T

Q

F

A

A

A

V

V

L

L

L

A

A

S

A

G

G

L

L

W

R

G

G

I

V

E

E

H
x
K

E

G

L

Y

E

V

P

L

T

G

P

P

P

Q

S

A

T

E

G

D

N

N

A

V

Y

W

V

S

-

L

-

T

-

Q

-

E

Q

R

K

R

V

A

S

M

G

G

E

Y

L

R

T

E

L

L

P

P

R

T

T

S

L

L

A

G

E

N

A

A

A

L

G

E

R

S

L

M

R

E

S

N

S

S

R

E

A

L

A

V

R

A

D

E

C

A

F

L

G

G

D

E

A

H

F

V

V

F

D

D

H

Y

F

F

A

L

S

R

T

N

R

K

-

R

-

S

E

E

W

W

E

E

D

N

R

-

E

-

Y

Y

R

R

R

S

H

H

V

V

S

T

D

P

W

Y

E

E

L

L

K

K

R

N

Y

Y

F

L

E

S

I

L

K363 (≠ H380) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Query Sequence

>WP_011876163.1 NCBI__GCF_000016205.1:WP_011876163.1
MMQARDVKTAADARKIVEERGLTHVEVAVTDLDGVLRGKYLSRDKFFSALENSFGFCSVL
FGWDCNDELYDEACKIEYTGWHNGYRDDEVRVIPESCRELPHKNSLLFLAEAHSEHGARV
CPRNLLSRVLARADELGFSVVSGFEYEFYGFNETPQSAAEKGYRDLKLLTPTSCGYSVLR
AAVQSELFQDLLKIGQVMDFPIEGLHTEAGKGAMEAALSLDTGIGSADKAVLFKTFSKAV
AQQRDITLSFMAKYSMEMPGCGGHIHLSLKDKDGKPVFFDAAQAHQMSKTMRHFIAGQQL
LMPEFLAMVAPTVNAYSRLVPGYWAPTSATWGVDNRTCALRVVGGSPKSKRIEYRVTSAD
TNPYLVQAAVLASGLWGIEHELEPTPPSTGNAYVQKVSGELTLPRTLAEAAGRLRSSRAA
RDCFGDAFVDHFASTREWEDREYRRHVSDWELKRYFEII

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory