SitesBLAST
Comparing WP_011882678.1 NCBI__GCF_000016205.1:WP_011882678.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3rrvB Crystal structure of an enoyl-coa hydratase/isomerase from mycobacterium paratuberculosis (see paper)
34% identity, 92% coverage: 12:253/262 of query aligns to 8:253/254 of 3rrvB
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
36% identity, 94% coverage: 12:257/262 of query aligns to 6:254/257 of 6slbAAA
- active site: Q64 (vs. gap), F69 (vs. gap), L80 (≠ I80), N84 (≠ T84), A108 (≠ L112), S111 (≠ T115), A130 (≠ T134), F131 (≠ H135), L136 (≠ V140), P138 (≠ A142), D139 (≠ G143), A224 (= A232), G234 (≠ F238)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (= R64), A62 (≠ S68), Q64 (vs. gap), D65 (vs. gap), L66 (vs. gap), Y76 (≠ D76), A108 (≠ L112), F131 (≠ H135), D139 (≠ G143)
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
36% identity, 94% coverage: 12:257/262 of query aligns to 3:242/245 of 6slaAAA
- active site: Q61 (≠ G70), L68 (≠ I80), N72 (≠ T84), A96 (≠ L112), S99 (≠ T115), A118 (≠ T134), F119 (≠ H135), L124 (≠ V140), P126 (≠ A142), N127 (≠ G143), A212 (= A232), G222 (≠ F238)
- binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (= L30), A59 (≠ S68), Q61 (≠ G70), D62 (= D71), L63 (≠ I75), L68 (≠ I80), Y71 (≠ A83), A94 (≠ V110), G95 (= G111), A96 (≠ L112), F119 (≠ H135), I122 (≠ A138), L124 (≠ V140), N127 (≠ G143), F234 (= F250), K237 (≠ R253)
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
32% identity, 96% coverage: 5:255/262 of query aligns to 1:252/258 of 1mj3A
- active site: A68 (≠ G70), M73 (≠ I75), S83 (≠ R85), L85 (≠ G87), G109 (≠ L112), E112 (≠ T115), P131 (≠ T134), E132 (≠ H135), T137 (≠ V140), P139 (≠ A142), G140 (= G143), K225 (≠ D228), F235 (= F238)
- binding hexanoyl-coenzyme a: K26 (≠ E28), A27 (≠ I29), L28 (= L30), A30 (= A32), A66 (≠ S68), G67 (= G69), A68 (≠ G70), D69 (= D71), I70 (≠ Q72), G109 (≠ L112), P131 (≠ T134), E132 (≠ H135), L135 (≠ A138), G140 (= G143)
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
32% identity, 95% coverage: 7:255/262 of query aligns to 2:248/254 of 2dubA
- active site: A67 (≠ G70), M72 (≠ I75), S82 (≠ R85), G105 (≠ L112), E108 (≠ T115), P127 (≠ T134), E128 (≠ H135), T133 (≠ V140), P135 (≠ A142), G136 (= G143), K221 (≠ D228), F231 (= F238)
- binding octanoyl-coenzyme a: K25 (≠ E28), A26 (≠ I29), L27 (= L30), A29 (= A32), A65 (≠ S68), A67 (≠ G70), D68 (= D71), I69 (≠ Q72), K70 (= K73), G105 (≠ L112), E108 (≠ T115), P127 (≠ T134), E128 (≠ H135), G136 (= G143), A137 (≠ D144)
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
31% identity, 96% coverage: 5:255/262 of query aligns to 1:254/260 of 1dubA
- active site: A68 (≠ G70), M73 (≠ I75), S83 (≠ R85), L87 (vs. gap), G111 (≠ L112), E114 (≠ T115), P133 (≠ T134), E134 (≠ H135), T139 (≠ V140), P141 (≠ A142), G142 (= G143), K227 (≠ D228), F237 (= F238)
- binding acetoacetyl-coenzyme a: K26 (≠ E28), A27 (≠ I29), L28 (= L30), A30 (= A32), A66 (≠ S68), A68 (≠ G70), D69 (= D71), I70 (≠ Q72), Y107 (≠ V108), G110 (= G111), G111 (≠ L112), E114 (≠ T115), P133 (≠ T134), E134 (≠ H135), L137 (≠ A138), G142 (= G143), F233 (≠ E234), F249 (= F250)
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
31% identity, 97% coverage: 3:255/262 of query aligns to 29:284/290 of P14604
- E144 (≠ T115) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (≠ H135) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
32% identity, 95% coverage: 7:255/262 of query aligns to 1:252/258 of 1ey3A
- active site: A66 (≠ G70), M71 (≠ I75), S81 (≠ R85), L85 (vs. gap), G109 (≠ L112), E112 (≠ T115), P131 (≠ T134), E132 (≠ H135), T137 (≠ V140), P139 (≠ A142), G140 (= G143), K225 (≠ D228), F235 (= F238)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ E28), L26 (= L30), A28 (= A32), A64 (≠ S68), G65 (= G69), A66 (≠ G70), D67 (= D71), I68 (≠ Q72), L85 (vs. gap), W88 (vs. gap), G109 (≠ L112), P131 (≠ T134), L135 (≠ A138), G140 (= G143)
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
32% identity, 96% coverage: 5:255/262 of query aligns to 1:254/260 of 2hw5C
- active site: A68 (≠ G70), M73 (≠ I75), S83 (≠ A83), L87 (≠ G87), G111 (≠ L112), E114 (≠ T115), P133 (≠ T134), E134 (≠ H135), T139 (≠ V140), P141 (≠ A142), G142 (= G143), K227 (≠ D228), F237 (= F238)
- binding crotonyl coenzyme a: K26 (≠ E28), A27 (≠ I29), L28 (= L30), A30 (= A32), K62 (≠ R64), I70 (≠ Q72), F109 (≠ V110)
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
29% identity, 95% coverage: 8:255/262 of query aligns to 2:253/259 of 5zaiC
- active site: A65 (≠ G70), F70 (≠ I75), S82 (≠ T84), R86 (= R88), G110 (≠ L112), E113 (≠ T115), P132 (≠ T134), E133 (≠ H135), I138 (≠ V140), P140 (≠ A142), G141 (= G143), A226 (= A232), F236 (= F238)
- binding coenzyme a: K24 (≠ I29), L25 (= L30), A63 (≠ S68), G64 (= G69), A65 (≠ G70), D66 (= D71), I67 (≠ Q72), P132 (≠ T134), R166 (≠ F168), F248 (= F250), K251 (≠ R253)
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
31% identity, 95% coverage: 7:254/262 of query aligns to 2:249/256 of 3h81A
- active site: A64 (≠ G70), M69 (≠ I75), T79 (≠ R85), F83 (≠ H89), G107 (≠ L112), E110 (≠ T115), P129 (≠ T134), E130 (≠ H135), V135 (= V140), P137 (≠ A142), G138 (= G143), L223 (≠ D228), F233 (= F238)
- binding calcium ion: F233 (= F238), Q238 (≠ R243)
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
31% identity, 95% coverage: 7:254/262 of query aligns to 3:250/255 of 3q0jC
- active site: A65 (≠ G70), M70 (≠ I75), T80 (≠ R85), F84 (≠ H89), G108 (≠ L112), E111 (≠ T115), P130 (≠ T134), E131 (≠ H135), V136 (= V140), P138 (≠ A142), G139 (= G143), L224 (≠ D228), F234 (= F238)
- binding acetoacetyl-coenzyme a: Q23 (≠ E28), A24 (≠ I29), L25 (= L30), A27 (= A32), A63 (≠ S68), G64 (= G69), A65 (≠ G70), D66 (= D71), I67 (≠ Q72), K68 (= K73), M70 (≠ I75), F84 (≠ H89), G107 (= G111), G108 (≠ L112), E111 (≠ T115), P130 (≠ T134), E131 (≠ H135), P138 (≠ A142), G139 (= G143), M140 (≠ D144)
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
31% identity, 95% coverage: 7:254/262 of query aligns to 3:250/255 of 3q0gC
- active site: A65 (≠ G70), M70 (≠ I75), T80 (≠ R85), F84 (≠ H89), G108 (≠ L112), E111 (≠ T115), P130 (≠ T134), E131 (≠ H135), V136 (= V140), P138 (≠ A142), G139 (= G143), L224 (≠ D228), F234 (= F238)
- binding coenzyme a: L25 (= L30), A63 (≠ S68), I67 (≠ Q72), K68 (= K73), Y104 (≠ V108), P130 (≠ T134), E131 (≠ H135), L134 (≠ A138)
2j5sA Structural of abdh, a beta-diketone hydrolase from the cyanobacterium anabaena sp. Pcc 7120 bound to (s)-3-oxocyclohexyl acetic acid (see paper)
29% identity, 89% coverage: 5:237/262 of query aligns to 8:244/250 of 2j5sA
- active site: I73 (≠ G70), L78 (≠ I75), T90 (≠ A83), K95 (≠ R88), L118 (= L112), H119 (≠ G113), E121 (≠ T115), P141 (vs. gap), H142 (= H135), G150 (= G143), G235 (≠ D228)
- binding (s)-cyclohexanone-2-acetate: H41 (= H38), F75 (≠ Q72), H119 (≠ G113), P141 (vs. gap)
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
31% identity, 95% coverage: 7:254/262 of query aligns to 2:245/250 of 3q0gD
- active site: A64 (≠ G70), M69 (≠ I75), T75 (≠ P81), F79 (vs. gap), G103 (≠ L112), E106 (≠ T115), P125 (≠ T134), E126 (≠ H135), V131 (= V140), P133 (≠ A142), G134 (= G143), L219 (≠ D228), F229 (= F238)
- binding Butyryl Coenzyme A: F225 (≠ E234), F241 (= F250)
1szoD Crystal structure analysis of the 6-oxo camphor hydrolase his122ala mutant bound to its natural product (2s,4s)-alpha-campholinic acid (see paper)
32% identity, 83% coverage: 4:221/262 of query aligns to 11:227/251 of 1szoD
- active site: I76 (≠ G70), F81 (≠ I75), I93 (≠ T84), Q97 (≠ R88), N120 (≠ L112), A121 (≠ G113), E123 (≠ T115), P143 (≠ T134), H144 (= H135), G152 (= G143)
- binding (2s,4s)-4-(2,2-dihydroxyethyl)-2,3,3-trimethylcyclopentanone: W39 (≠ I33), F81 (≠ I75), I92 (≠ A83), H144 (= H135), D153 (= D144)
Sites not aligning to the query:
Q93TU6 6-oxocamphor hydrolase; OCH; Beta-diketone hydrolase; EC 3.7.1.18 from Rhodococcus sp. (see paper)
32% identity, 83% coverage: 4:221/262 of query aligns to 12:228/257 of Q93TU6