SitesBLAST

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
34% identity, 54% coverage: 70:179/202 of query aligns to 74:186/203 of P07464

query
sites
P07464

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N85 (= N81) binding in other chain; binding in other chain
D93 (≠ R89) binding substrate
H115 (≠ L107) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ A135) binding in other chain
A160 (= A153) binding in other chain
TK 165:166 (= TK 158:159) binding acetyl-CoA
R180 (≠ K173) binding acetyl-CoA
R183 (≠ G176) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding substrate
71 binding substrate

3nz2J Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
36% identity, 51% coverage: 72:175/202 of query aligns to 75:181/185 of 3nz2J

query
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3nz2J

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binding acetyl coenzyme *a: G104 (≠ S101), Y110 (≠ L107), H114 (= H111), W138 (= W132), G140 (= G134), A158 (= A152), A159 (= A153), N164 (≠ T158), G174 (= G168), T176 (≠ V170), P177 (= P171)

Sites not aligning to the query:

binding acetyl coenzyme *a: 182

3nz2C Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
36% identity, 51% coverage: 72:175/202 of query aligns to 72:178/183 of 3nz2C

query
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3nz2C

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binding acetyl coenzyme *a: Y107 (≠ L107), H111 (= H111), G137 (= G134), A155 (= A152), A156 (= A153), N161 (≠ T158), G171 (= G168), T173 (≠ V170), P174 (= P171), L178 (≠ I175)
binding magnesium ion: N157 (≠ G154), T173 (≠ V170)

Sites not aligning to the query:

binding acetyl coenzyme *a: 179

3ectA Crystal structure of the hexapeptide-repeat containing- acetyltransferase vca0836 from vibrio cholerae
36% identity, 51% coverage: 72:175/202 of query aligns to 65:171/176 of 3ectA

query
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3ectA

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binding calcium ion: G138 (= G142), D156 (= D160)

A1ADJ6 Polysialic acid O-acetyltransferase; Capsule O-acetyl transferase; EC 2.3.1.136 from Escherichia coli O1:K1 / APEC (see paper)
31% identity, 65% coverage: 49:179/202 of query aligns to 169:280/307 of A1ADJ6

query
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A1ADJ6

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H210 (≠ I92) mutation to A: Loss of activity.
W234 (= W132) mutation to A: Loss of activity.

6pubA Crystal structure of the type b chloramphenicol acetyltransferase from vibrio cholerae in the complex with crystal violet
50% identity, 33% coverage: 117:182/202 of query aligns to 105:169/210 of 6pubA

query
sites
6pubA

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Sites not aligning to the query:

binding crystal violet: 4, 7, 9, 10, 11, 36, 37

6u9cA The 2.2 a crystal structure of the type b chloramphenicol acetyltransferase from vibrio cholerae in the complex with acetyl coa
50% identity, 33% coverage: 117:182/202 of query aligns to 102:166/206 of 6u9cA

query
sites
6u9cA

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binding acetyl coenzyme *a: W116 (= W132), A136 (= A152), I159 (= I175)

8j40A Crystal structure of catb8 in complex with chloramphenicol (see paper)
43% identity, 30% coverage: 122:181/202 of query aligns to 107:166/209 of 8j40A

query
sites
8j40A

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binding chloramphenicol: W117 (= W132), V135 (= V150), S138 (≠ A153)

Sites not aligning to the query:

binding chloramphenicol: 7, 8, 9, 29, 31, 47, 50, 90, 187, 190, 191, 202, 203, 206

4hurA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with acetyl coenzyme a (see paper)
44% identity, 31% coverage: 119:181/202 of query aligns to 107:169/211 of 4hurA

query
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4hurA

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binding acetyl coenzyme *a: K110 (≠ G122), W120 (= W132), I121 (≠ L133), G122 (= G134), R123 (≠ A135), M128 (≠ L140), A140 (= A152), A141 (= A153), T146 (= T158), G156 (= G168), P159 (= P171), I163 (= I175), R164 (≠ G176)

Sites not aligning to the query:

binding acetyl coenzyme *a: 51, 67, 68, 69, 79, 80

6x3cA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
44% identity, 31% coverage: 119:181/202 of query aligns to 107:169/207 of 6x3cA

query
sites
6x3cA

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binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: W120 (= W132), G122 (= G134), R123 (≠ A135), M128 (≠ L140), A140 (= A152), A141 (= A153)

Sites not aligning to the query:

4husA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with virginiamycin m1 (see paper)
44% identity, 31% coverage: 119:181/202 of query aligns to 107:169/212 of 4husA

query
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4husA

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Sites not aligning to the query:

binding virginiamycin m1: 17, 36, 38

6x3jA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f0224 (46) (see paper)
44% identity, 31% coverage: 119:181/202 of query aligns to 107:169/206 of 6x3jA

query
sites
6x3jA

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Sites not aligning to the query:

6x3cE Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
44% identity, 31% coverage: 119:181/202 of query aligns to 107:169/203 of 6x3cE

query
sites
6x3cE

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G

R

Q

K

R

R

N

F

P

S

D

D

binding magnesium ion: N158 (≠ V170), P159 (= P171)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: W120 (= W132), G122 (= G134), A140 (= A152), A141 (= A153)

Sites not aligning to the query:

1mrlA Crystal structure of streptogramin a acetyltransferase with dalfopristin (see paper)
45% identity, 30% coverage: 119:178/202 of query aligns to 108:167/204 of 1mrlA

query
sites
1mrlA

M
x
L

S

P

I

I

G

K

G

G

I

D

V

T

T

I

I

I

H

G

D

N

H

D

A

V

W

W

L

I

G

G

A

K

R

D

A

V

I

V

I

I

L

M

P

P

G

G

V

V

T

K

I

I

G

G

K

D

G

G

A

A

V

I

V

V

A

A

G

N

A

S

V

V

T

V

K

K

D

D

V

I

P

A

D

P

F

Y

T

M

V

L

V

A

G

G

V

N

P

P

A

A

K

N

I

E

I

I

G

K

Q

Q

R

R

binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: L108 (≠ M119)

Sites not aligning to the query:

binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: 37, 39, 54, 56, 81, 82, 93, 102

Query Sequence

>WP_011883536.1 NCBI__GCF_000016205.1:WP_011883536.1
MKSRIKARLLASSTYFFLAALKYYIGNNFIARMPIERVRNFYYRRVLGVLIGQRTHVSMR
QFFTGYHTRCNVRIGDNCVLNREIYLDGRTGIEIGNNVNISFQCCLLSLHHDHNHPGFMS
IGGIVTIHDHAWLGARAIILPGVTIGKGAVVAAGAVVTKDVPDFTVVGGVPAKIIGQRNP
DVAYLTDFSPFFDTDVFDESRG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory