SitesBLAST
Comparing WP_011885180.1 NCBI__GCF_000016205.1:WP_011885180.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4g6zA Crystal structure of a glutamyl-tRNA synthetase glurs from burkholderia thailandensis bound to l-glutamate (see paper)
84% identity, 81% coverage: 3:384/469 of query aligns to 1:365/380 of 4g6zA
P04805 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Escherichia coli (strain K12) (see 4 papers)
50% identity, 99% coverage: 5:468/469 of query aligns to 3:461/471 of P04805
- C98 (= C100) mutation to S: 10-fold decrease in activity. Strong decrease in zinc content.
- C100 (≠ M102) mutation to S: Loss of activity. Strong decrease in zinc content.; mutation to Y: Does not prevent zinc binding. Reduces only 2-fold the binding affinity for tRNA(Glu), but reduces more than 10-fold the affinity for glutamate in the presence of tRNA(Glu).
- C125 (≠ W127) mutation to S: Loss of activity. Strong decrease in zinc content.
- H127 (≠ P129) mutation to Q: 10-fold decrease in activity. Strong decrease in zinc content.
- H129 (≠ P131) mutation to Q: No change in activity or in zinc content.
- H131 (≠ K133) mutation to Q: No change in activity or in zinc content.
- H132 (≠ V134) mutation to Q: No change in activity or in zinc content.
- C138 (≠ A140) mutation to S: No change in activity or in zinc content.
- S239 (= S245) modified: Phosphoserine; mutation to D: Does not aminoacylate tRNA(Glu), not phosphorylated by HipA.
8i9iA Glutamyl-tRNA synthetase from escherichia coli bound to glutamate and zinc
50% identity, 99% coverage: 5:468/469 of query aligns to 3:461/468 of 8i9iA
2cfoA Non-discriminating glutamyl-tRNA synthetase from thermosynechococcus elongatus in complex with glu (see paper)
43% identity, 81% coverage: 5:386/469 of query aligns to 2:393/484 of 2cfoA
Q8DLI5 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1) (see paper)
43% identity, 81% coverage: 5:386/469 of query aligns to 3:394/485 of Q8DLI5
- R6 (= R8) binding L-glutamate
- Y192 (= Y187) binding L-glutamate
4griB Crystal structure of a glutamyl-tRNA synthetase glurs from borrelia burgdorferi bound to glutamic acid and zinc (see paper)
33% identity, 99% coverage: 5:467/469 of query aligns to 2:478/485 of 4griB
- active site: S9 (= S12), K253 (= K246)
- binding glutamic acid: R5 (= R8), A7 (= A10), S9 (= S12), E41 (= E44), Y194 (= Y187), R212 (= R205), W216 (≠ H209)
- binding zinc ion: C105 (= C100), C107 (≠ M102), Y128 (= Y123), C132 (≠ W127)
1g59A Glutamyl-tRNA synthetase complexed with tRNA(glu). (see paper)
35% identity, 99% coverage: 5:469/469 of query aligns to 2:468/468 of 1g59A
- binding : D44 (= D47), R45 (≠ V48), A46 (≠ E49), R47 (= R50), P109 (≠ A104), V145 (= V145), R163 (≠ K163), V166 (= V166), E172 (= E172), V177 (= V177), K180 (≠ R180), S181 (≠ P181), D182 (= D182), E207 (≠ D207), E208 (≠ D208), R237 (≠ L237), K241 (≠ G241), T242 (≠ E242), K243 (= K243), M273 (≠ L273), G274 (= G274), E282 (= E281), S299 (≠ G298), L300 (≠ K299), P303 (≠ A302), V304 (≠ Q303), K309 (= K308), W312 (= W311), R319 (≠ K318), P357 (≠ D358), R358 (= R359), R417 (≠ K418), K426 (= K427), L427 (≠ M428), Q432 (≠ M433), R435 (= R436), L442 (≠ T443), E443 (≠ H444), T444 (= T445), P445 (= P446), G446 (≠ S447), L447 (≠ I448), F448 (≠ D449)
2cv2A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu) and an enzyme inhibitor, glu-ams (see paper)
35% identity, 99% coverage: 5:469/469 of query aligns to 2:468/468 of 2cv2A
- active site: K246 (= K246)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R5 (= R8), A7 (= A10), S9 (= S12), G17 (= G20), I21 (≠ S24), E41 (= E44), Y187 (= Y187), R205 (= R205), A206 (≠ G206), E208 (≠ D208), W209 (≠ H209), L235 (≠ T235), L236 (≠ V236)
- binding : S9 (= S12), T43 (= T46), D44 (= D47), R47 (= R50), V145 (= V145), R163 (≠ K163), Y168 (≠ I168), E172 (= E172), V177 (= V177), K180 (≠ R180), S181 (≠ P181), Y187 (= Y187), E207 (≠ D207), E208 (≠ D208), W209 (≠ H209), V211 (≠ N211), R237 (≠ L237), K241 (≠ G241), L272 (≠ R272), M273 (≠ L273), G274 (= G274), E282 (= E281), S299 (≠ G298), P303 (≠ A302), V304 (≠ Q303), K309 (= K308), W312 (= W311), R319 (≠ K318), P357 (≠ D358), R358 (= R359), R417 (≠ K418), Q432 (≠ M433), R435 (= R436), L442 (≠ T443), E443 (≠ H444), T444 (= T445), G446 (≠ S447), L447 (≠ I448), F448 (≠ D449)
2cv1A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu), atp, and an analog of l-glutamate: a quaternary complex
35% identity, 99% coverage: 5:469/469 of query aligns to 2:468/468 of 2cv1A
- active site: K246 (= K246)
- binding adenosine-5'-triphosphate: P8 (= P11), S9 (= S12), G17 (= G20), T18 (≠ N21), I21 (≠ S24), R47 (= R50), A206 (≠ G206), W209 (≠ H209), L235 (≠ T235), L236 (≠ V236)
- binding (4s)-4-amino-5-hydroxypentanoic acid: R5 (= R8), A7 (= A10), E41 (= E44), Y187 (= Y187), R205 (= R205), W209 (≠ H209)
- binding : S9 (= S12), E41 (= E44), T43 (= T46), D44 (= D47), R47 (= R50), V145 (= V145), R163 (≠ K163), V166 (= V166), E172 (= E172), V177 (= V177), K180 (≠ R180), S181 (≠ P181), Y187 (= Y187), E207 (≠ D207), E208 (≠ D208), W209 (≠ H209), V211 (≠ N211), R237 (≠ L237), K241 (≠ G241), K243 (= K243), M273 (≠ L273), G274 (= G274), S276 (= S276), E282 (= E281), S299 (≠ G298), P303 (≠ A302), V304 (≠ Q303), K309 (= K308), W312 (= W311), R319 (≠ K318), P357 (≠ D358), R358 (= R359), R417 (≠ K418), L427 (≠ M428), Q432 (≠ M433), R435 (= R436), L442 (≠ T443), E443 (≠ H444), T444 (= T445), G446 (≠ S447), L447 (≠ I448), F448 (≠ D449)
2cuzA Glutamyl-tRNA synthetase from thermus thermophilus in complex with l- glutamate (see paper)
35% identity, 99% coverage: 5:469/469 of query aligns to 2:468/468 of 2cuzA
1n78A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with tRNA(glu) and glutamol-amp. (see paper)
35% identity, 99% coverage: 5:469/469 of query aligns to 2:468/468 of 1n78A
- active site: K246 (= K246)
- binding glutamol-amp: R5 (= R8), A7 (= A10), P8 (= P11), S9 (= S12), G17 (= G20), T18 (≠ N21), I21 (≠ S24), E41 (= E44), Y187 (= Y187), N191 (≠ V191), R205 (= R205), A206 (≠ G206), E208 (≠ D208), W209 (≠ H209), L235 (≠ T235), L236 (≠ V236)
- binding : S9 (= S12), T43 (= T46), D44 (= D47), R47 (= R50), V145 (= V145), R163 (≠ K163), V166 (= V166), Y168 (≠ I168), E172 (= E172), V177 (= V177), K180 (≠ R180), S181 (≠ P181), Y187 (= Y187), E207 (≠ D207), E208 (≠ D208), W209 (≠ H209), L210 (≠ V210), V211 (≠ N211), R237 (≠ L237), K241 (≠ G241), M273 (≠ L273), G274 (= G274), E282 (= E281), R297 (≠ H296), P303 (≠ A302), V304 (≠ Q303), K309 (= K308), W312 (= W311), R319 (≠ K318), P357 (≠ D358), R358 (= R359), R417 (≠ K418), L427 (≠ M428), Q432 (≠ M433), R435 (= R436), L442 (≠ T443), E443 (≠ H444), T444 (= T445), G446 (≠ S447), L447 (≠ I448), F448 (≠ D449)
1j09A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with atp and glu (see paper)
35% identity, 99% coverage: 5:469/469 of query aligns to 2:468/468 of 1j09A
- active site: K246 (= K246)
- binding adenosine-5'-triphosphate: H15 (= H18), E208 (≠ D208), L235 (≠ T235), L236 (≠ V236), K243 (= K243), I244 (≠ M244), S245 (= S245), K246 (= K246), R247 (= R247)
- binding glutamic acid: R5 (= R8), A7 (= A10), S9 (= S12), E41 (= E44), Y187 (= Y187), N191 (≠ V191), R205 (= R205), W209 (≠ H209)
P27000 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
35% identity, 99% coverage: 5:469/469 of query aligns to 2:468/468 of P27000
- R358 (= R359) mutation to Q: Reduces affinity for tRNA and abolishes the ability to discriminate between tRNA(Glu) and tRNA(Gln).
6brlA Crystal structure of a glutamate tRNA ligase from elizabethkingia meningosepticum ccug26117 in complex with its amino acid (see paper)
32% identity, 99% coverage: 5:468/469 of query aligns to 3:498/502 of 6brlA
3al0C Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
32% identity, 99% coverage: 5:468/469 of query aligns to 103:559/564 of 3al0C
- active site: S110 (= S12), K335 (= K246)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R106 (= R8), A108 (= A10), P109 (= P11), G118 (= G20), T122 (≠ S24), E142 (= E44), Y276 (= Y187), R294 (= R205), G295 (= G206), D297 (= D208), H298 (= H209), L324 (≠ T235), I325 (≠ V236), L333 (≠ M244)
- binding : T144 (= T46), D145 (= D47), R148 (= R50), Y208 (≠ A104), P213 (≠ A109), K252 (= K163), M255 (≠ V166), I266 (≠ V177), K269 (≠ R180), S270 (≠ P181), Y276 (= Y187), D297 (= D208), H298 (= H209), L299 (≠ V210), S300 (≠ N211), N301 (= N212), K304 (≠ R215), R330 (≠ G241), P332 (≠ K243), G363 (= G274), W364 (= W275), R365 (≠ S276), E370 (= E281), S387 (≠ G298), K389 (≠ S300), V391 (≠ A302), I392 (≠ Q303), K397 (= K308), W400 (= W311), R407 (≠ K318), E446 (≠ D358), K447 (≠ R359), Q453 (≠ E365), I457 (≠ G369), R509 (≠ K418), K520 (≠ Q430), Q524 (≠ P434), R527 (= R436), V535 (≠ H444), T536 (= T445), G538 (≠ S447), L539 (≠ I448)
8vc5A Crystal structure of glutamyl-tRNA synthetase glurs from pseudomonas aeruginosa (zinc bound)
33% identity, 99% coverage: 5:468/469 of query aligns to 4:466/488 of 8vc5A
P27305 Glutamyl-Q tRNA(Asp) synthetase; Glu-Q-RSs; EC 6.1.1.- from Escherichia coli (strain K12) (see paper)
37% identity, 52% coverage: 8:250/469 of query aligns to 19:245/308 of P27305
- E55 (= E44) binding L-glutamate
- Y182 (= Y187) binding L-glutamate
- R200 (= R205) binding L-glutamate
4a91A Crystal structure of the glutamyl-queuosine trnaasp synthetase from e. Coli complexed with l-glutamate (see paper)
37% identity, 52% coverage: 8:251/469 of query aligns to 7:234/290 of 4a91A
- active site: S11 (= S12), K229 (= K246)
- binding glutamic acid: R7 (= R8), A9 (= A10), S11 (= S12), E43 (= E44), Y170 (= Y187), R188 (= R205), L192 (≠ H209)
- binding zinc ion: C99 (= C100), C101 (≠ M102), Y113 (= Y123), C117 (≠ W127)
3aiiA Archaeal non-discriminating glutamyl-tRNA synthetase from methanothermobacter thermautotrophicus (see paper)
31% identity, 58% coverage: 8:280/469 of query aligns to 14:284/455 of 3aiiA
P46655 Glutamate--tRNA ligase, cytoplasmic; Glutamyl-tRNA synthetase; (c)ERS; GluRS; P85; EC 6.1.1.17 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
26% identity, 47% coverage: 5:223/469 of query aligns to 202:418/708 of P46655
Sites not aligning to the query:
- 148 R→A: Abolishes interaction with ARC1.
Query Sequence
>WP_011885180.1 NCBI__GCF_000016205.1:WP_011885180.1
MTRPVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGTFVLRIEDTDVERSSQEAVDAIL
EGMQWLGLDFDEGPIYQMQRMDRYRAVLAQMLEQGLAYPCYMSAEELDALRERQRAAGEK
PRYDGTWRPEPGKVLPEPPAGVKPVLRFRNPLTGTVVWDDAVKGRVEISNEELDDLVIAR
PDGTPIYNFCVVVDDMDMNITHVIRGDDHVNNTPRQINILRALGGEPPVYAHLPTVLNEQ
GEKMSKRHGAMSVMAYRDAGFLPEAVVNYLARLGWSHGDAEIFSREQFVEWFDLEHLGKS
PAQYDHSKLSWLNAHYIKEADNARLAALAKPFLDALGIDDAAIATGPALDAVIGLMKDRA
TTVKEIAEGAAMFYRVPAPDADALAQHVTDAVRPALADLAAALKAADWTKEAVSAALKAT
LATHKLKMPQLAMPVRLLVAGTTHTPSIDAVLVLFGRDAVVSRIEAALA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory