SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011886233.1 NCBI__GCF_000016205.1:WP_011886233.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P78061 Gamma-glutamylputrescine synthetase PuuA; Gamma-Glu-Put synthetase; Glutamate--putrescine ligase; EC 6.3.1.11 from Escherichia coli (strain K12) (see paper)
34% identity, 99% coverage: 2:442/445 of query aligns to 25:469/472 of P78061

query
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P78061

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H282 (= H254) mutation to N: Activity is impaired to 9% of wild-type.
R357 (= R331) mutation to Q: Activity is impaired to 3% of wild-type.

8ooqB Glutamine synthetase from Methanothermococcus thermolithotrophicus (see paper)
33% identity, 85% coverage: 61:437/445 of query aligns to 56:439/446 of 8ooqB

query
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8ooqB

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binding 2-oxoglutaric acid: R86 (vs. gap), V92 (≠ T91), P169 (≠ D175), R172 (≠ F178), R173 (≠ E179), S189 (≠ L195)
binding magnesium ion: E137 (= E135), E192 (= E198), E199 (= E205)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 16, 18, 32

8oooA Glutamine synthetase from methanothermococcus thermolithotrophicus in complex with 2-oxoglutarate and mgatp at 2.15 a resolution (see paper)
33% identity, 85% coverage: 61:437/445 of query aligns to 57:440/447 of 8oooA

query
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8oooA

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binding 2-oxoglutaric acid: R87 (vs. gap), V93 (≠ T91), P170 (≠ D175), R173 (≠ F178), R174 (≠ E179), S190 (≠ L195)
binding adenosine-5'-triphosphate: E136 (= E133), E188 (≠ D193), F203 (= F208), K204 (≠ M209), F205 (≠ H210), H251 (= H256), S253 (= S258), R325 (= R331), R335 (= R341)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 17, 19, 33

8tfkA Glutamine synthetase (see paper)
30% identity, 98% coverage: 2:437/445 of query aligns to 4:432/440 of 8tfkA

query
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8tfkA

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D

D

I

V

E

L

D

E

F

A

L

V

K

K

Q

E

H

R

R

D

I

V

T

K

E

F

I

I

E

R

A

T

I

Q

I

F

P

T

D

D

M

T

A

L

G

G

I

I

A

I

R

K

G

S

K

W

I

A

T

I

P

P

R

A

N

E

K

Q

F

L

T

E

S

E

G

A

E

-

S

-

M

-

R

-

L

F

P

E

Q

N

A

G

V

V

M

M

V

F

Q

-

T

-

V

-

T

-

G

-

E

-

Y

-

P

-

E

-

D

D

G

G

S

S

-

S

-

I

-

Q

-

G

L

F

T

T

G

R

V

I

T

E

D

E

P

S

D

D

M

M

V

K

C

L

V

A

P

L

D

D

T

P

S

S

T

T

I

F

R

R

L

I

I

L

P

P

W

W

-

R

-

P

A

A

V

T

D

G

P

A

T

V

A

A

Q

R

V

I

I

L

H

G

D

D

C

V

V

Y

H

L

F

P

D

D

G

G

S

N

P

P

V

F

E

K

I

G

S

D

P

P

R

R

Y

Y

V

V

L

L

R

K

R

T

V

A

L

I

E

K

L

E

Y

A

K

E

A

K

K

M

G

G

W

F

K

S

P

M

V

N

V

V

A

G

P

P

E
|
E

L

L

E
|
E

F

F

Y

F

L

L

-

F

-

K

V

L

D

D

M

A

N

N

K

G

D

N

P

P

D

T

L

T

P

E

L

L

R

T

P

D

P

Q

V

G

G

G

R

Y

T

-

G

-

R

-

P

-

E

-

T

-

G

-

R

-

Q

-

S

-

Y

F

S

D

I

F

E

A

A

P

V

L

N

D

E

R

F

A

D

Q

P

D

L

V

F

R

E

R

D

D

I

I

Y

D

E

Y

Y

A

C

L

E

E

M

H

Q

M

N

G

L

F

D

Q

I

I

D

E

T

A

L

S

I

H

H

H

E
|
E

V

V

G

A

A

P

A

S

Q

Q

M

H

E
|
E

I

I

N
x
D

F
|
F

M

R

H
x
F

G

G

D

D

A

V

L

L

S

C

L

T

A

A

D

D

Q

N

V

V

F

V

L

T

F

F

K

K

R

Y

T

V

V

V

R

K

E

S

A

I

A

A

L

Y

R

H

H

K

S

G

M

Y

Y

Y

A

A

T

S

F

F

M

M

A

P

K

K

P

P

M

L

E

F

G

G

E

V

P

N

G
|
G

S

S

A

G

M

M

H
|
H

V

S

H
x
N

Q

Q

S

S

I

L

V

F

D

K

E

D

E

-

T

-

G

G

R

K

N

N

L

V

F

F

T

Y

S

D

P

P

E

D

T

T

G

-

A

P

T

T

S

K

L

L

F

S

Y

Q

N

D

-

A

-

M

-

Y

Y

Y

L

I

A

G

G

G

L

L

Q

L

K

K

Y

H

T

I

P

R

A

E

L

F

M

T

P

A

I

V

F

T

A

N

P

P

Y

V

I

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

S

V

R

P

F

G

M

Y

A
x
E

A

A

P

P

I

V

N

Y

V

I

Q

S

W

W

G

S

Y

A

D

Q

N

N

R
|
R

T

S

V

S

G

L

F

I

R
|
R

I

I

P

P

H

A

S

T

G

R

P

G

A

N

A
x
G

R

T

R
|
R

I

I

E
|
E

N

L

R
|
R

I

C

P

P

G

D

V

P

D

A

C

C

N

N

P

P

Y

Y

L

L

A

A

L

F

A

A

A

L

T

M

L

L

A

R

A

A

G

G

Y

L

L

E

G

G

M

I

T

K

Q

N

R

K

L

I

E

D

P

P

T

G

E

E

P

P

L

T

V

N

S

V

D

N

G

I

Y

F

S

H

L

L

P

S

Y

D

Q

K

-

E

-

R

-

E

-

R

-

G

-

I

-

R

-

S

L

L

P

P

R

A

N

D

L

L

E

K

E

E

G

A

L

I

T

D

L

E

M

M

A

K

A

G

C

S

E

K

P

F

L

V

A

K

G

E

I

A

L

L

G

G

D

E

K

H

F

V

L

F

K

S

A

H

Y

Y

F

L

A

C

L

A

K

K

E

E

T

M

E

E

Y

W

E

D

A

E

F

Y

F

K

R

A

V

V

I

V

S

H

S

P

W

W

E

E

binding adenosine-5'-diphosphate: E128 (= E133), D194 (≠ N207), F195 (= F208), F197 (≠ H210), N243 (≠ H256), R312 (= R326), R317 (= R331), G325 (≠ A339), R327 (= R341)
binding magnesium ion: E128 (= E133), E128 (= E133), E130 (= E135), E185 (= E198), E192 (= E205), E192 (= E205), H241 (= H254), E329 (= E343)
binding l-methionine-s-sulfoximine phosphate: E128 (= E133), E130 (= E135), E185 (= E198), E192 (= E205), G237 (= G250), H241 (= H254), R294 (= R308), E300 (≠ A314), R312 (= R326), R331 (= R345)

8ufjB Glutamine synthetase (see paper)
30% identity, 99% coverage: 2:442/445 of query aligns to 8:441/444 of 8ufjB

query
sites
8ufjB

Q

E

D

D

I

V

E

L

D

E

F

A

L

V

K

K

Q

E

H

R

R

D

I

V

T

K

E

F

I

I

E

R

A

T

I

Q

I

F

P

T

D

D

M

T

A

L

G

G

I

I

A

I

R

K

G

S

K

W

I

A

T

I

P

P

R

A

N

E

K

Q

F

L

T

E

S

E

G

A

E

-

S

-

M

-

R

-

L

F

P

E

Q

N

A

G

V

V

M

M

V

F

Q

-

T

-

V

-

T

-

G

-

E

-

Y

-

P

-

E

-

D

D

G

G

S

S

-

S

-

I

-

Q

-

G

L

F

T

T

G

R

V

I

T

E

D

E

P

S

D

D

M

M

V

K

C

L

V

A

P

L

D

D

T

P

S

S

T

T

I

F

R

R

L

I

I

L

P

P

W

W

-

R

-

P

A

A

V

T

D

G

P

A

T

V

A

A

Q

R

V

I

I

L

H

G

D

D

C

V

V

Y

H

L

F

P

D

D

G

G

S

N

P

P

V

F

E

K

I

G

S

D

P

P

R

R

Y

Y

V

V

L

L

R

K

R

T

V

A

L

I

E

K

L

E

Y

A

K

E

A

K

K

M

G

G

W

F

K

S

P

M

V

N

V

V

A

G

P

P

E
|
E

L

L

E
|
E

F

F

Y

F

L

L

-

F

-

K

V

L

D

D

M

A

N

N

K

G

D

N

P

P

D

T

L

T

P

E

L

L

R

T

P

D

P

Q

V

G

G

G

R

Y

T

-

G

-

R

-

P

-

E

-

T

-

G

-

R

-

Q

-

S

-

Y

F

S

D

I

F

E

A

A

P

V

L

N

D

E

L

F

G

D

Q

P

D

L

V

F

R

E

R

D

D

I

I

Y

D

E

Y

Y

A

C

L

E

E

M

H

Q

M

N

G

L

F

D

Q

I

I

D

E

T

A

L

S

I

H

H

H

E
|
E

V

V

G

A

A

P

A

S

Q

Q

M

H

E
|
E

I

I

N

D

F

F

M

R

H

F

G

G

D

D

A

V

L

L

S

C

L

T

A

A

D

D

Q

N

V

V

F

V

L

T

F

F

K

K

R

Y

T

V

V

V

R

K

E

S

A

I

A

A

L

Y

R

H

H

K

S

G

M

Y

Y

Y

A

A

T

S

F

F

M

M

A

P

K

K

P

P

M

L

E

F

G

G

E

V

P

N

G

G

S

S

A

G

M

M

H
|
H

V

S

H

N

Q

Q

S

S

I

L

V

F

D

K

E

D

E

-

T

-

G

G

R

K

N

N

L

V

F

F

T

Y

S

D

P

P

E

D

T

T

G

-

A

P

T

T

S

K

L

L

F

S

Y

Q

N

D

-

A

-

M

-

Y

Y

Y

L

I

A

G

G

G

L

L

Q

L

K

K

Y

H

T

I

P

R

A

E

L

F

M

T

P

A

I

V

F

T

A

N

P

P

Y

V

I

V

N

N

S

S

Y

Y

R

K

R

R

L

L

S

V

R

P

F

G

M

Y

A

E

A

A

P

P

I

V

N

Y

V

I

Q

S

W

W

G

S

Y

A

D

Q

N

N

R

R

T

S

V

S

G

L

F

I

R

R

I

I

P

P

H

A

S

T

G

R

P

G

A

N

A

G

R

T

R

R

I

I

E
|
E

N

L

R

R

I

C

P

P

G

D

V

P

D

A

C

C

N

N

P

P

Y

Y

L

L

A

A

L

F

A

A

A

L

T

M

L

L

A

R

A

A

G

G

Y

L

L

E

G

G

M

I

T

K

Q

N

R

K

L

I

E

D

P

P

T

G

E

E

P

P

L

T

V

N

S

V

D

N

G

I

Y

F

S

H

L

L

P

S

Y

D

Q

K

-

E

-

R

-

E

-

R

-

G

-

I

-

R

-

S

L

L

P

P

R

A

N

D

L

L

E

K

E

E

G

A

L

I

T

D

L

E

M

M

A

K

A

G

C

S

E

K

P

F

L

V

A

K

G

E

I

A

L

L

G

G

D

E

K

H

F

V

L

F

K

S

A

H

Y

Y

F

L

A

C

L

A

K

K

E

E

T

M

E

E

Y

W

E

D

A

E

F

Y

F

K

R

A

V

V

I

V

S

H

S

P

W

W

E

E

R

L

R

S

H

R

L

Y

L

L

binding magnesium ion: E132 (= E133), E134 (= E135), E189 (= E198), E196 (= E205), H245 (= H254), E333 (= E343)

7tdpA Structure of paenibacillus polymyxa gs bound to met-sox-p-adp (transition state complex) to 1.98 angstom (see paper)
31% identity, 90% coverage: 41:442/445 of query aligns to 31:436/439 of 7tdpA

query
sites
7tdpA

E

K

S

N

M

V

R

E

L

I

P

P

Q

V

A

S

V

Q

M

L

V

E

Q

K

T

A

V

L

T

D

G

N

E

K

Y

M

P

M

E

F

D

D

G

G

S

S

-

S

-

I

-

E

-

G

L

Y

T

V

G

R

V

I

T

E

D

E

P

S

D

D

M

M

V

Y

C

L

V

Y

P

P

D

D

T

L

S

D

T

T

I

W

R

V

L

V

I

F

P

P

W

W

A

S

V

-

D

D

P

R

T

V

A

A

Q

R

V

L

I

I

H

C

D

D

C

I

V

Y

H

K

F

P

D

D

G

G

S

S

P

P

V

F

E

A

I

G

S

D

P

P

R

R

Y

G

V

I

L

L

R

K

R

R

V

V

L

L

E

K

L

E

Y

A

K

E

A

E

K

L

G

G

W

Y

K

T

P

S

V

M

-
x
N

V

V

A
x
G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

-

F

-

K

V

T

D

D

M

E

N

K

K

G

D

D

P

P

D

T

L

T

P

E

L

L

R

N

P

D

P

Q

V

G

G

G

R

-

T

-

G

-

R

-

P

-

E

-

T

-

G

-

R

-

Q

-

S

Y

Y

F

S

D

I

L

E

A

A

P

V

M

N

D

E

L

F

G

D

E

P

N

L

C

F

R

E

R

D

E

I

I

Y

V

E

L

Y

K

C

L

E

E

M

E

Q

M

N

G

L

F

D

E

I

I

D
x
E

T

A

L

S

I

H

H

H

E
|
E

V

V

G

A

A

P

A

G

Q

Q

M

H

E
|
E

I

I

N
x
D

F

F

M

K

H
x
Y

G

A

D

D

A

A

L

V

S

K

L

A

A

A

D

D

Q

Q

V

I

F

Q

L

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

A

I

A

A

L

R

R

Q

H

H

S

G

M

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

E

F

G

G

E

V

P

N

G
|
G

S

S

A

G

M

M

H
|
H

V

C

H
x
N

Q

Q

S
|
S

I

L

V

F

D

K

E

D

E

-

T

-

G

N

R

E

N

N

L

V

F

F

-

Y

T

D

S

E

P

T

E

D

T

E

G

L

G

G

A

L

T

S

S

Q

L

T

F

A

Y

R

N

H

Y

Y

L

M

A

A

G

G

L

I

Q

L

K

K

Y

H

T

A

P

R

A

A

L

M

M

A

P

A

I

I

F

T

A

N

P

P

Y

T

I

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

S

V

R

P

F

G

M

Y

A
x
E

A

A

P

P

I

C

N

Y

V

V

Q

A

W

W

G

S

Y

A

D

S

N

N

R
|
R

T

S

V

P

G

M

F

I

R
|
R

I

I

P

P

H

A

S

S

G

R

P

G

A

L

A

S

R

T

R
|
R

I

V

E
|
E

N

V

R
|
R

I

N

P

P

G

D

V

P

D

A

C

A

N

N

P

P

Y

Y

L

L

A

A

L

L

A

A

A

V

T

M

L

L

A

R

A

A

G

G

Y

L

L

D

G

G

M

I

T

K

Q

R

R

Q

L

M

E

A

P

L

T

P

E

A

P

P

L

I

V

D

S

R

D

N

G

I

Y

Y

S

V

L

M

P

S

Y

E

Q

E

-

E

-

R

-

I

-

E

-

G

-

I

-

P

-

S

L

L

P

P

R

A

N

D

L

L

E

K

E

E

G

A

L

L

T

S

L

E

M

L

A

I

A

R

C

S

E

E

P

V

L

I

A

S

G

D

I

A

L

L

G

G

D

D

K

H

F

A

L

L

K

A

A

Y

Y

F

F

Y

A

E

L

L

K

K

E

E

T

I

E

E

Y

W

E

D

A

M

F

Y

F

R

R

T

V

Q

I

V

S

H

S

Q

W

W

E

E

R

R

R

D

H

Q

L

Y

L

L

binding adenosine-5'-diphosphate: N123 (vs. gap), G125 (≠ A131), E127 (= E133), E179 (≠ D193), D193 (≠ N207), Y196 (≠ H210), N242 (≠ H256), S244 (= S258), R316 (= R331), R326 (= R341)
binding magnesium ion: E127 (= E133), E127 (= E133), E129 (= E135), E184 (= E198), E191 (= E205), E191 (= E205), H240 (= H254), E328 (= E343)
binding l-methionine-s-sulfoximine phosphate: E127 (= E133), E129 (= E135), E184 (= E198), E191 (= E205), G236 (= G250), H240 (= H254), R293 (= R308), E299 (≠ A314), R311 (= R326), R330 (= R345)

7tfaB Glutamine synthetase (see paper)
31% identity, 90% coverage: 41:442/445 of query aligns to 31:438/441 of 7tfaB

query
sites
7tfaB

E

K

S

N

M

V

R

E

L

I

P

P

Q

V

A

S

V

Q

M

L

V

E

Q

K

T

A

V

L

T

D

G

N

E

K

Y

M

P

M

E

F

D

D

G

G

S

S

-

S

-

I

-

E

-

G

L
x
Y

T

V

G
x
R

V

I

T

E

D

E

P

S

D

D

M

M

V

Y

C

L

V

Y

P

P

D

D

T

L

S

D

T

T

I

W

R

V

L

V

I

F

P

P

W

W

A

V

V

T

-

S

D

D

P

R

T

V

A

A

Q

R

V

L

I

I

H

C

D

D

C

I

V

Y

H

K

F

P

D

D

G

G

S

S

P

P

V

F

E

A

I

G

S

D

P

P

R

R

Y

G

V

I

L

L

R

K

R

R

V

V

L

L

E

K

L

E

Y

A

K

E

A

E

K

L

G

G

W

Y

K

T

P

S

V

M

-

N

V

V

A

G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

-

F

-

K

V

T

D

D

M

E

N

K

K

G

D

D

P

P

D

T

L

T

P

E

L

L

R

N

P

D

P

Q

V

G

G

G

R

-

T

-

G

-

R

-

P

-

E

-

T

-

G

-

R

-

Q

-

S
x
Y

Y

F

S

D

I

L

E

A

A

P

V

M

N

D

E

L

F

G

D

E

P

N

L

C

F

R

E

R

D

E

I

I

Y

V

E

L

Y

K

C

L

E

E

M

E

Q

M

N

G

L

F

D

E

I

I

D

E

T

A

L

S

I

H

H

H

E
|
E

V
|
V

G

A

A

P

A

G

Q

Q

M

H

E
|
E

I

I

N

D

F

F

M

K

H

Y

G

A

D

D

A

A

L

V

S

K

L

A

A

A

D

D

Q

Q

V

I

F

Q

L

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

A

I

A

A

L

R

R

Q

H

H

S

G

M

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

E

F

G

G

E

V

P
x
N

G
|
G

S

S

A

G

M

M

H
|
H

V

C

H

N

Q

Q

S

S

I

L

V

F

D

K

E

D

E

-

T

-

G

N

R

E

N

N

L

V

F

F

-

Y

T

D

S

E

P

T

E

D

T

E

G

L

G

G

A

L

T

S

S

Q

L

T

F

A

Y

R

N

H

Y

Y

L

M

A

A

G

G

L

I

Q

L

K

K

Y

H

T

A

P

R

A

A

L

M

M

A

P

A

I

I

F

T

A

N

P

P

Y

T

I

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

S

V

R

P

F
x
G

M
x
Y

A
x
E

A

A

P

P

I

C

N

Y

V

V

Q

A

W

W

G

S

Y

A

D

S

N

N

R
|
R

T

S

V

P

G

M

F

I

R

R

I

I

P

P

H

A

S

S

G

R

P

G

A

L

A

S

R

T

R

R

I

V

E
|
E

N

V

R

R

I

N

P

P

G

D

V

P

D

A

C

A

N

N

P

P

Y

Y

L

L

A

A

L

L

A

A

A

V

T

M

L

L

A

R

A

A

G

G

Y

L

L

D

G

G

M

I

T

K

Q

R

R

Q

L

M

E

A

P

L

T

P

E

A

P

P

L

I

V

D

S

R

D

N

G

I

Y

Y

S

V

L

M

P

S

Y

E

Q

E

-

E

-

R

-

I

-

E

-

G

-

I

-

P

-

S

L

L

P

P

R

A

N

D

L

L

E

K

E

E

G

A

L

L

T

S

L

E

M

L

A

I

A

R

C

S

E

E

P

V

L

I

A

S

G

D

I

A

L

L

G

G

D

D

K

H

F

A

L

L

K

A

A

Y

Y

F

F

Y

A

E

L

L

K

K

E

E

T

I

E

E

Y

W

E

D

A
x
M

F

Y

F

R

R

T

V

Q

I

V

S

H

S

Q

W

W

E

E

R

R

R

D

H

Q

L

Y

L

L

binding glutamine: E131 (= E135), Y153 (≠ S165), E186 (= E198), G238 (= G250), H242 (= H254), R295 (= R308), E301 (≠ A314)
binding magnesium ion: E129 (= E133), E131 (= E135), E186 (= E198), E193 (= E205), H242 (= H254), E330 (= E343)
binding : Y58 (≠ L64), R60 (≠ G66), V187 (= V199), N237 (≠ P249), G299 (≠ F312), Y300 (≠ M313), R313 (= R326), M424 (≠ A428)

8oozA Glutamine synthetase (see paper)
31% identity, 85% coverage: 61:437/445 of query aligns to 50:422/430 of 8oozA

query
sites
8oozA

D

D

G

G

S

S

L

-

T

-

G

S

V

I

T

E

D

G

P

S

D

D

M

M

V

V

C

L

V

R

P

P

D

D

T

P

S

D

T

T

I

F

R

R

L

V

I

L

P

P

W

W

A

S

V

T

D

-

P

A

T

E

A

A

Q

R

V

L

I

I

H

C

D

D

C

I

V

E

H

L

F

P

D

D

G

G

S

K

P

P

V

F

E

M

I

G

S

C

P

P

R

R

Y

Q

V

V

L

L

R

K

V

N

L

M

E

E

L

E

Y

A

K

A

A

K

K

L

G

G

W

Y

K

V

P

M

V

N

V

T

A
x
G

P

P

E
|
E

L

M

E

E

F

F

Y

F

L

L

V

-

D

-

M

F

N

K

K

R

D

Q

P

D

D

G

L

M

P

P

L

T

R

N

P

I

P

P

V

Q

G

D

R

R

T

G

G

G

R

-

P

-

E

-

T

-

G

-

R

-

Q

-

S

Y

Y

F

S

D

I

L

E

A

A

P

V

I

N

D

E

L

F

A

D

E

P

E

L

I

F

K

E

R

D

E

I

I

Y

V

E

L

Y

V

C

L

E

E

M

E

Q

M

N

G

L

F

D

E

I

V

D
x
E

T

A

L

A

I

H

H

H

E

E

V

V

G

A

A

F

A

G

Q

Q

M

H

E

E

I

I

N

D

F
|
F

M
x
K

H
x
Y

G

D

D

N

A

A

L

L

S

A

L

T

A

A

D

D

Q

N

V

V

F

I

L

T

F

L

K

K

R

Y

T

V

V

A

R

K

E

T

A

L

A

A

L

L

R

Q

H

H

S

G

M

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

I

E

F

G

G

E

V

P

N

G

G

S

S

A

G

M

M

H
|
H

V

T

H
x
N

Q

T

S
|
S

I

L

V

F

D

K

E

D

E

-

T

-

G

G

R

K

N

N

L

A

F

F

T

Y

S

D

P

P

E

D

T

A

G

P

G

D

A

Q

T

I

S

S

L

D

F

T

Y

L

N

R

Y

Y

-

F

L

V

A

G

G

G

L

V

Q

L

K

K

Y

H

T

I

P

R

A

A

L

I

M

T

P

A

I

I

F

T

A

N

P

P

Y

L

I

V

N

N

S

S

Y

Y

R

K

R

R

L

L

S

V

R

P

F

G

M

Y

A

E

A

A

P

P

I

V

N

Y

V

I

Q

T

W

W

G

S

Y

G

D

P

N

N

R

R

T

S

V

S

G

L

F

I

R

R

I

V

P

P

H

A

S

P

G

R

P

G

A

N

A
x
S

R

T

R
|
R

I

I

E
|
E

N

I

R

R

I

S

P

P

G

D

V

P

D

S

C

C

N

N

P

P

Y

Y

L

L

A

A

L

F

A

A

T

I

L

L

A

A

G

G

Y

L

L

D

G

G

M

V

T

K

Q

N

R

K

L

I

E

E

P

P

T

P

E

E

P

R

L

V

V

E

S

K

D

N

G

I

Y

Y

S

K

L

L

P

T

Y

E

Q

E

-

E

-

R

-

E

-

K

-

L

-

G

-

I

-

G

-

M

L

L

P

P

R

G

N

T

L

L

E

K

E

E

G

A

L

I

T

E

L

C

M

F

A

K

A

E

C

D

E

E

P

L

L

L

A

V

G

S

I

A

L

L

G

G

D

E

K

H

F

V

L

S

K

Q

A

S

Y

I

F

I

A

N

L

V

K

A

E

M

T

A

E

D

Y

W

E

D

A

S

F

Y

F

R

R

T

V

Q

I

V

S

H

S

Q

W

W

E

E

binding adenosine-5'-triphosphate: G117 (≠ A131), E170 (≠ D193), F185 (= F208), K186 (≠ M209), Y187 (≠ H210), N233 (≠ H256), S235 (= S258), S315 (≠ A339), R317 (= R341)
binding magnesium ion: E119 (= E133), H231 (= H254), E319 (= E343)

8ooxB Glutamine synthetase (see paper)
30% identity, 83% coverage: 67:437/445 of query aligns to 60:430/438 of 8ooxB

query
sites
8ooxB

V

I

T

E

D

E

P

S

D

D

M

M

V

V

C

L

V

R

P

P

D

D

T

P

S

D

T

T

I

F

R

R

L

V

I

L

P

P

W

W

A

S

V

G

D

N

P

T

T

A

-

E

A

A

Q

R

V

L

I

I

H

C

D

D

C

I

V

E

H

L

F

P

D

D

G

G

S

K

P

P

V

F

E

M

I

G

S

C

P

P

R

R

Y

Q

V

V

L

L

R

K

V

N

L

M

E

E

L

E

Y

A

K

A

A

K

K

L

G

G

W

Y

K

V

P

M

V

N

V

T

A

G

P

P

E

E

L

M

E
|
E

F

F

Y

F

L

L

V

-

D

-

M

F

N

K

K

R

D

Q

P

D

D

G

L

M

P

P

L

T

R

N

P

I

P

P

V

Q

G

D

R

R

T

G

G

G

R

-

P

-

E

-

T

-

G

-

R

-

Q

-

S

Y

Y

F

S

D

I

L

E

A

A

P

V

I

N

D

E

L

F

A

D

E

P

E

L

I

F

K

E

R

D

E

I

I

Y

V

E

L

Y

V

C

L

E

E

M

E

Q

M

N

G

L

F

D

E

I

V

D

E

T

A

L

A

I

H

H

H

E
|
E

V

V

G

A

A

F

A

G

Q

Q

M

H

E
|
E

I

I

N

D

F

F

M

K

H

Y

G

D

D

N

A

A

L

L

S

A

L

T

A

A

D

D

Q

N

V

V

F

I

L

T

F

L

K

K

R

Y

T

V

V

A

R

K

E

T

A

L

A

A

L

L

R

Q

H

H

S

G

M

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

I

E

F

G

G

E

V

P

N

G

G

S

S

A

G

M

M

H

H

V

T

H

N

Q

T

S

S

I

L

V

F

D

K

E

D

E

-

T

-

G

G

R

K

N

N

L

A

F

F

T

Y

S

D

P

P

E

D

T

A

G

P

G

D

A

Q

T

I

S

S

L

D

F

T

Y

L

N

R

Y

Y

-

F

L

V

A

G

G

G

L

V

Q

L

K

K

Y

H

T

I

P

R

A

A

L

I

M

T

P

A

I

I

F

T

A

N

P

P

Y

L

I

V

N

N

S

S

Y

Y

R

K

R

R

L

L

S

V

R

P

F

G

M

Y

A

E

A

A

P

P

I

V

N

Y

V

I

Q

T

W

W

G

S

Y

G

D

P

N

N

R

R

T

S

V

S

G

L

F

I

R

R

I

V

P

P

H

A

S

P

G

R

P

G

A

N

A

S

R

T

R

R

I

I

E

E

N

I

R

R

I

S

P

P

G

D

V

P

D

S

C

C

N

N

P

P

Y

Y

L

L

A

A

L

F

A

A

T

I

L

L

A

A

G

G

Y

L

L

D

G

G

M

V

T

K

Q

N

R

K

L

I

E

E

P

P

T

P

E

E

P

R

L

V

V

E

S

K

D

N

G

I

Y

Y

S

K

L

L

P

T

Y

E

Q

E

-

E

-

R

-

E

-

K

-

L

-

G

-

I

-

G

-

M

L

L

P

P

R

G

N

T

L

L

E

K

E

E

G

A

L

I

T

E

L

C

M

F

A

K

A

E

C

D

E

E

P

L

L

L

A

V

G

S

I

A

L

L

G

G

D

E

K

H

F

V

L

S

K

Q

A

S

Y

I

F

I

A

N

L

V

K

A

E

M

T

A

E

D

Y

W

E

D

A

S

F

Y

F

R

R

T

V

Q

I

V

S

H

S

Q

W

W

E

E

binding magnesium ion: E129 (= E135), E183 (= E198), E190 (= E205)

7tdvC Glutamine synthetase (see paper)
28% identity, 99% coverage: 2:442/445 of query aligns to 6:440/443 of 7tdvC

query
sites
7tdvC

Q

E

D

D

I

I

E

R

D

K

F

F

L

V

K

E

Q

E

H

E

R

N

I

V

T

R

E

Y

I

L

E

R

A

L

I

Q

I

F

P

T

D

D

M

I

A

L

G

G

I

T

A

I

R

K

G

-

K

-

I

-

T

-

P

-

R

-

N

-

K

-

F

-

T

-

S

-

G

-

E

-

S

N

M

V

R

E

L

V

P

P

Q

V

A

S

V

Q

M

L

V

E

Q

K

T

V

V

L

T

D

G

N

E

E

Y

M

P

M

E

F

D

D

G

G

S

S

-

S

-

I

-

E

-

G

L

F

T

V

G

R

V

I

T

E

D

E

P

S

D

D

M

M

V

Y

C

L

V

H

P

P

D

D

T

L

S

D

T

T

I

W

R

V

L

I

I

F

P

P

W

W

A

T

V

A

D

G

-

Q

-

G

P

K

T

V

A

A

Q

R

V

L

I

I

H

C

D

D

C

V

V

Y

H

K

F

T

D

D

G

G

S

T

P

P

V

F

E

E

I

G

S

D

P

P

R

R

Y

A

V

N

L

L

R

K

R

R

V

V

L

L

E

K

L

E

Y

M

K

E

A

D

K

L

G

G

W

F

K

T

P

D

V

F

-

N

V

L

A
x
G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

-

F

-

K

V

L

D

D

M

E

N

K

K

G

D

E

P

P

D

T

L

L

P

E

L

L

R

N

P

D

V

G

G

G

R

-

T

-

G

-

R

-

P

-

E

-

T

-

G

-

R

-

Q

-

S

Y

Y

F

S

D

I

L

E

A

A

P

V

T

N

D

E

L

F

G

D

E

P

N

L

C

F

R

E

R

D

D

I

I

Y

V

E

L

Y

E

C

L

E

E

M

D

Q

M

N

G

L

F

D

D

I

I

D
x
E

T

A

L

S

I

H

H

H

E
|
E

V

V

G

A

A

P

A

G

Q

Q

M

H

E
|
E

I

I

N
x
D

F
|
F

M
x
K

H
x
Y

G

A

D

D

A

A

L

V

S

T

L

A

A

C

D

D

Q

N

V

I

F

Q

L

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

A

I

A

A

L

R

R

K

H

H

S

N

M

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

E

F

G

G

E

V

P

N

G
|
G

S

S

A

G

M

M

H
|
H

V

F

H
x
N

Q
x
V

S
|
S

I

L

V

F

D

-

E

-

E

K

T

G

G

K

R

E

N

N

L

A

F

F

T

F

S

D

P

P

E

N

T

T

G

E

-

M

G

G

A

L

T

T

S

E

L

T

F

A

Y

Y

N

Q

Y

F

L

T

A

A

G

G

L

V

Q

L

K

K

Y

N

T

A

P

R

A

G

L

F

M

T

P

A

I

V

F

C

A

N

P

P

Y

L

I

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

S

V

R

P

F

G

M

Y

A
x
E

A

A

P

P

I

C

N

Y

V

I

Q

A

W

W

G

S

Y

G

D

K

N

N

R
|
R

T

S

V

P

G

L

F

I

R
|
R

I

V

P

P

H

S

S

S

G

R

P

G

A

L

A
x
S

R

T

R
|
R

I

I

E
|
E

N

V

R

R

I

S

P

V

G

D

V

P

D

A

C

A

N

N

P

P

Y

Y

L

M

A

A

L

L

A

A

T

I

L

L

A

E

A

A

G

G

Y

L

L

D

G

G

M

I

T

K

Q

N

R

K

L

L

E

K

P

V

T

P

E

E

P

P

L

V

V

N

S

Q

D

N

G

I

Y

Y

S

E

L

M

P

N

Y

R

Q

E

-

E

-

R

-

E

-

A

-

V

-

G

-

I

-

Q

-

D

L

L

P

P

R

S

N

T

L

L

E

Y

E

T

G

A

L

L

T

K

L

A

M

M

A

R

A

E

C

N

E

E

P

V

L

I

A

K

G

K

I

A

L

L

G

G

D

N

K

H

F

I

L

Y

K

N

A

Q

Y

F

F

I

A

N

L

S

K

K

E

S

T

I

E

E

Y

W

E

D

A

Y

F

Y

F

R

R

T

V

Q

I

V

S

S

S

E

W

W

E

E

R

R

R

D

H

Q

L

Y

L

M

binding adenosine-5'-diphosphate: G129 (≠ A131), E131 (= E133), E183 (≠ D193), D197 (≠ N207), F198 (= F208), K199 (≠ M209), Y200 (≠ H210), N246 (≠ H256), V247 (≠ Q257), S248 (= S258), R320 (= R331), S328 (≠ A339), R330 (= R341)
binding magnesium ion: E131 (= E133), E131 (= E133), E133 (= E135), E188 (= E198), E195 (= E205), E195 (= E205), H244 (= H254), E332 (= E343)
binding l-methionine-s-sulfoximine phosphate: E131 (= E133), E133 (= E135), E188 (= E198), E195 (= E205), G240 (= G250), H244 (= H254), R297 (= R308), E303 (≠ A314), R315 (= R326)

7tf6A Glutamine synthetase (see paper)
28% identity, 99% coverage: 2:442/445 of query aligns to 5:435/438 of 7tf6A

query
sites
7tf6A

Q

E

D

D

I

I

E

R

D

K

F

F

L

A

K

E

Q

E

H

E

R

N

I

V

T

R

E

Y

I

L

E

R

A

L

I

Q

I

F

P

T

D

D

M

I

A

L

G

G

I

T

A

I

R

K

G

-

K

-

I

-

T

-

P

-

R

-

N

-

K

-

F

-

T

-

S

-

G

-

E

-

S

N

M

V

R

E

L

V

P

P

Q

V

A

S

V

Q

M

L

V

E

Q

K

T

V

V

L

T

D

G

N

E

E

Y

M

P

M

E

F

D

D

G

G

S

S

-

S

-

I

-

E

-

G

L
x
F

T

V

G
x
R

V

I

T

E

D

E

P

S

D

D

M

M

V

Y

C

L

V

H

P

P

D

D

T

L

S

D

T

T

I

W

R

V

L

I

I

F

P

P

W

W

A

G

V

-

D

-

P

K

T

V

A

A

Q

R

V

L

I

I

H

C

D

D

C

V

V

Y

H

K

F

T

D

D

G

G

S

T

P

P

V

F

E

E

I

G

S

D

P

P

R

R

Y

A

V

N

L

L

R

K

R

R

V

V

L

L

E

K

L

E

Y

M

K

E

A

D

K

L

G

G

W

F

K

T

P

D

V

F

-

N

V

L

A

G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

-

F

-

K

V

L

D

D

M

E

N

K

K

G

D

E

P

P

D

T

L

L

P

E

L

L

R

N

P

D

V

G

G

G

R

-

T

-

G

-

R

-

P

-

E

-

T

-

G

-

R

-

Q

-

S

Y

Y

F

S

D

I

L

E

A

A

P

V

T

N

D

E

L

F

G

D

E

P

N

L

C

F

R

E

R

D

D

I

I

Y

V

E

L

Y

E

C

L

E

E

M

D

Q

M

N

G

L

F

D

D

I

I

D

E

T

A

L

S

I

H

H

H

E
|
E

V

V

G

A

A

P

A

G

Q

Q

M

H

E
|
E

I

I

N

D

F

F

M

K

H

Y

G

A

D

D

A

A

L

V

S

T

L

A

A

C

D

D

Q

N

V

I

F

Q

L

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

A

I

A

A

L

R

R

K

H

H

S

N

M

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

E

F

G
|
G

E

V

P
x
N

G
|
G

S

S

A

G

M

M

H
|
H

V

F

H

N

Q

V

S

S

I

L

V

F

D

-

E

-

E

K

T

G

G

K

R

E

N

N

L

A

F

F

T

F

S

D

P

P

E

N

T

T

G

E

-

M

G

G

A

L

T

T

S

E

L

T

F

A

Y

Y

N

Q

Y

F

L

T

A

A

G

G

L

V

Q

L

K

K

Y

N

T

A

P

R

A

G

L

F

M

T

P

A

I

V

F

C

A

N

P

P

Y

L

I

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

S

V

R

P

F
x
G

M
x
Y

A
x
E

A

A

P

P

I

C

N

Y

V

I

Q

A

W

W

G

S

Y

G

D

K

N

N

R
|
R

T

S

V

P

G

L

F

I

R

R

I

V

P

P

H

S

S

S

G

R

P

G

A

L

A

S

R

T

R

R

I

I

E
|
E

N

V

R

R

I

S

P

V

G

D

V

P

D

A

C

A

N

N

P

P

Y

Y

L

M

A

A

L

L

A

A

T

I

L

L

A

E

A

A

G

G

Y

L

L

D

G

G

M

I

T

K

Q

N

R

K

L

L

E

K

P

V

T

P

E

E

P

P

L

V

V

N

S

Q

D

N

G

I

Y
|
Y

S

E

L

M

P

N

Y

R

Q

E

-

E

-

R

-

E

-

A

-

V

-

G

-

I

-

Q

-

D

L

L

P

P

R

S

N

T

L

L

E

Y

E

T

G

A

L

L

T

K

L

A

M

M

A

R

A

E

C

N

E

E

P

V

L

I

A

K

G

K

I

A

L

L

G

G

D

N

K

H

F

I

L

Y

K

N

A

Q

Y

F

F

I

A

N

L

S

K

K

E

S

T

I

E

E

Y

W

E

D

A
x
Y

F

Y

F

R

R

T

V

Q

I

V

S

S

S

E

W

W

E

E

R

R

R

D

H
x
Q

L

Y

L

M

binding glutamine: E128 (= E135), E183 (= E198), G235 (= G250), H239 (= H254), R292 (= R308), E298 (≠ A314)
binding magnesium ion: E126 (= E133), E128 (= E135), E183 (= E198), E190 (= E205), H239 (= H254), E327 (= E343)
binding : F58 (≠ L64), R60 (≠ G66), G232 (= G247), N234 (≠ P249), G296 (≠ F312), Y297 (≠ M313), R310 (= R326), Y367 (= Y383), Y421 (≠ A428), Q433 (≠ H440)

Sites not aligning to the query:

binding : 437

5dm3C Crystal structure of glutamine synthetase from chromohalobacter salexigens dsm 3043(csal_0679, target efi-550015) with bound adp
33% identity, 81% coverage: 76:437/445 of query aligns to 58:390/396 of 5dm3C

query
sites
5dm3C

P

P

D

D

T

L

S

A

T

T

I

L

R

R

L

C

I

V

P

P

W

W

A

-

V

L

D

E

P

G

T

T

A

A

Q

M

V

V

I

L

H

C

D

D

C

L

V

L

-

D

H

H

F

R

D

T

G

H

S

A

P

E

V

V

E

P

I

H

S

A

P

P

R

R

Y

A

V

I

L

L

R

K

R

R

V

Q

L

L

E

A

L

R

Y

L

K

E

A

A

K

M

G

G

W

L

K

E

P

A

V

I

V

M

A

A

P

T

E
|
E

L

L

E
|
E

F

F

Y

F

L

L

V

F

D

E

M

K

N

S

K

L

D

D

P

E

D

I

L

-

P

-

L

-

R

-

P

-

V

-

G

-

R

R

T

K

G

G

R

R

P

F

E

R

T

T

G

T

R

K

Q

E

S

E

Y

H

S

V

I

L

E

R

A

-

V

-

N

-

E

-

F

-

D

-

P

P

L

L

F

R

E

N

D

H

I

L

Y

H

E

-

Y

-

C

-

E

-

M

A

Q

A

N

G

L

I

D

P

I

V

D

E

T

G

L

T

I

K

H

G

E
|
E

V

A

G

G

A

A

A

G

Q

Q

M

E

E
|
E

I

L

N

N

F

I

M
x
R

H
x
C

G

A

D

K

A

A

L

L

S

D

L

T

A

A

D

D

Q

Y

V

H

F

T

L

I

F

A

K

K

R

H

T

A

V

T

R

K

E

E

A

I

A

A

L

W

R

Q

H

Q

S

G

M

R

Y

A

A

V

T

T

F

F

M

L

A

S

K

K

P

W

M

H

E

H

G

A

E

H

P

A

G

G

S

S

A

S

M

S

H
|
H

V

I

H
|
H

Q

Q

S
|
S

I

L

V

W

D

K

E

Q

E

-

T

-

G

G

R

L

N

P

L

A

F

F

T

H

S

D

P

E

E

R

T

D

G

A

-

L

G

G

A

M

T

S

S

A

L

L

F

M

Y

K

N

H

Y

Y

L

L

A

A

G

G

L

L

Q

L

K

K

Y

Y

T

A

P

P

A

D

L

Y

M

T

P

Y

I

F

F

L

A

A

P

P

Y

Y

I

L

N

N

S

S

Y

Y

R

K

R

R

L

-

S

-

R

-

F

F

M

Q

A

F

A

A

P

P

I

T

N

R

V

T

Q

V

W

W

G

S

Y

V

D

D

N

N

R
|
R

T

T

V

A

G

G

F

F

R

R

I

L

P

C

H

A

S

E

G

G

P

T

A

R

A

A

R

V

R
|
R

I

I

E
|
E

N

C

R
|
R

I

I

P

G

G

G

V

S

D

D

C

L

N

N

P

P

Y

Y

L

L

A

A

L

M

A

A

A

G

T

Q

L

L

A

A

G

G

Y

I

L

K

G

G

M

I

T

E

Q

E

R

C

L

L

E

A

P

L

T

P

E

P

P

P

L

-

V

-

S

A

D

S

G

G

Y

L

S

-

L

-

P

-

Y

-

Q

-

L

I

P

P

R

Q

N

N

L

L

E

R

E

D

G

A

L

M

T

E

L

A

M

L

A

R

A

G

C

S

E

T

P

M

L

L

A

R

G

E

I

A

L

M

G

G

D

E

K

D

F

V

L

V

K

D

A

H

Y

Y

F

V

A

R

L

A

K

A

E

E

T

V

E

E

Y

L

E

E

A

D

F

F

F

Q

R

R

V

V

I

V

S

S

S

D

W

Y

E

E

active site: E115 (= E133), E117 (= E135), E162 (= E198), E169 (= E205), H218 (= H254), R286 (= R326), E303 (= E343), R305 (= R345)
binding adenosine-5'-diphosphate: R173 (≠ M209), C174 (≠ H210), H220 (= H256), S222 (= S258), R301 (= R341)

A0R083 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
31% identity, 84% coverage: 67:442/445 of query aligns to 62:444/446 of A0R083

query
sites
A0R083

V

V

T

F

D

E

P

S

D

D

M

T

V

V

C

A

V

R

P

P

D

D

T

P

S

S

T

T

I

F

R

Q

L

V

I

L

P

P

W

W

A

K

V

T

D

S

P

D

-

G

-

N

-

H

-

Y

T

S

A

A

Q

R

V

M

I

F

H

C

D

D

C

I

V

T

H

M

F

P

D

D

G

G

S

S

P

P

V

S

E

W

I

A

S

D

P

S

R

R

Y

H

V

V

L

L

R

R

V

Q

L

L

E

A

L

K

Y

A

K

S

A

D

K

L

G

G

W

F

K

T

P

C

V

Y

V

V

A

H

P

P

E

E

L

I

E

E

F

F

Y

F

L

L

V

L

D

K

M

P

N

G

K

P

D

N

P

D

D

G

L

T

P

P

L

P

R

E

P

P

P

-

V

-

G

-

R

-

T

-

G

-

R

-

P

-

E

-

T

A

G

D

R

N

Q

G

S

G

Y

Y

S

F

I

D

E

Q

A

A

V

V

N

H

E

D

F

A

D

A

P

P

L

N

F

F

E

R

-

R

D

H

I

A

Y

I

E

E

Y

A

C

L

E

E

M

Q

Q

M

N

G

L

I

D

S

I

V

D

E

T

F

L

S

I

H

H

H

E

E

V

G

G

A

A

P

A

G

Q

Q

M

Q

E

E

I

I

N

D

F

L

M

R

H

Y

G

A

D

D

A

A

L

L

S

S

L

M

A

A

D

D

Q

N

V

V

F

M

L

T

F

F

K

R

R

Y

T

L

V

V

R

K

E

E

A

V

A

A

L

L

R

A

H

D

S

G

M

V

Y

R

A

A

T

S

F

F

M

M

A

P

K

K

P

P

M

F

E

A

G

E

E

H

P

P

G

G

S

S

A

A

M

M

H

H

V

T

H

H

Q

M

S

S

I

L

V

F

D

E

E

G

E

D

T

T

G

-

R

-

N

N

L

A

F

F

T

H

S

S

P

P

E

D

T

D

G

P

G

L

A

Q

T

L

S

S

-

D

L

V

F

A

Y

K

N

S

Y

F

L

I

A

A

G

G

L

I

Q

L

K

E

Y

H

T

A

P

N

A

E

L

I

M

S

P

A

I

V

F

T

A

N

P

Q

Y

W

I

V

N

N

S

S

Y

Y

R

K

R

R

L

L

S

V

R

H

F

G

M

G

A

E

A

A

P

P

I

T

N

A

V

A

Q

S

W

W

G

G

Y

A

D

A

N

N

R

R

T

S

V

A

G

L

F

V

R

R

I

V

P

P

H

M

S

Y

G

T

P

P

-

H

-

K

-

V

A

S

R

R

I

V

E

E

N

V

R

R

I

S

P

P

G

D

V

S

D

A

C

C

N

N

P

P

Y

Y

L

L

A

T

L

F

A

A

A

V

T

L

L

L

A

A

G

G

Y

L

L

R

G

G

M

V

T

E

Q
x
K

R

G

L

Y

E

V

P

L

T

G

E

P

P

Q

L

A

V

E

S

D

D

N

G

V

Y

W

S

S

L

L

P

T

Y

Q

-

E

-

R

-

A

-

M

-

G

-

Y

-

R

Q

E

L

L

P

P

R

T

N

S

L

L

E

G

E

N

G

A

L

L

T

E

L

S

M

M

A

E

A

N

C

S

E

E

P

L

L

V

A

A

G

E

I

A

L

L

G

G

D

E

K

H

F

V

L

F

K

D

A

Y

Y

F

F

L

A

R

L

N

K

K

E

R

T

S

E

E

Y

W

E

E

A

N

F

Y

F

R

R

S

V

H

I

V

S

T

S

P

W

Y

E

E

R

L

R

K

H

N

L

Y

L

L

K363 (≠ Q370) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

P9WN37 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
32% identity, 84% coverage: 67:442/445 of query aligns to 62:444/446 of P9WN37

query
sites
P9WN37

V

V

T

S

D

E

P

S

D

D

M

T

V

V

C

A

V

H

P

P

D

D

T

P

S

S

T

T

I

F

R

Q

L

V

I

L

P

P

W

W

A

A

V

T

D

S

P

S

-

G

-

H

T

S

A

A

Q

R

V

M

I

F

H

C

D

D

C

I

V

T

H

M

F

P

D

D

G

G

S

S

P

P

V

S

E

W

I

A

S

D

P

P

R

R

Y

H

V

V

L

L

R

R

V

Q

L

L

E

T

L

K

Y

A

K

G

A

E

K

L

G

G

W

F

K

S

P

C

V

Y

V

V

A

H

P

P

E

E

L

I

E

E

F

F

Y

F

L

L

V

L

D

K

M

P

N

G

-

P

K

E

D

D

P

G

D

S

L

V

P

P

L

V

R

-

P

-

P

P

V

V

G

D

R

N

T

A

G

G

R

-

P

-

E

-

T

-

G

-

R

-

Q

-

S

-

Y

Y

S

F

I

D

E

Q

A

A

V

V

N

H

E

D

F

S

D

A

P

L

L

N

F

F

E

R

-

R

D

H

I

A

Y

I

E

D

Y

A

C

L

E

E

M

F

Q

M

N

G

L

I

D

S

I

V

D

E

T

F

L

S

I

H

H

H

E

E

V

G

G

A

A

P

A

G

Q

Q

M

Q

E

E

I

I

N

D

F

L

M

R

H

F

G

A

D

D

A

A

L

L

S

S

L

M

A

A

D

D

Q

N

V

V

F

M

L

T

F

F

K

R

R

Y

T

V

V

I

R

K

E

E

A

V

A

A

L

L

R

E

H

E

S

G

M

A

Y

R

A

A

T

S

F

F

M

M

A

P

K

K

P

P

M

F

E

G

G

Q

E

H

P

P

G

G

S

S

A

A

M

M

H

H

V

T

H

H

Q

M

S

S

I

L

V

F

D

E

E

G

E

D

T

V

G

-

R

-

N

N

L

A

F

F

T

H

S

S

P

A

E

D

T

D

G

P

G

L

A

Q

T

L

S

S

-

E

L

V

F

G

Y

K

N

S

Y

F

L

I

A

A

G

G

L

I

Q

L

K

E

Y

H

T

A

P

C

A

E

L

I

M

S

P

A

I

V

F

T

A

N

P

Q

Y

W

I

V

N

N

S

S

Y

Y

R

K

R

R

L

L

S

V

R

Q

F

G

M

G

A

E

A

A

P

P

I

T

N

A

V

A

Q

S

W

W

G

G

Y

A

D

A

N

N

R

R

T

S

V

A

G

L

F

V

R

R

I

V

P

P

H

M

S

Y

G

T

P

P

-

H

-

K

-

T

A

S

R

R

I

V

E

E

N

V

R

R

I

S

P

P

G

D

V

S

D

A

C

C

N

N

P

P

Y

Y

L

L

A

T

L

F

A

A

A

V

T

L

L

L

A

A

G

G

Y

L

L

R

G

G

M

V

T

E

Q
x
K

R

G

-

Y

-

V

L

L

E

G

P

P

-

Q

T

A

E

E

P

D

L

N

V

V

S

W

D

D

-

L

-

T

-

P

-

E

-

R

-

A

-

M

G

G

Y

Y

S

R

L

-

P

-

Y

-

Q

E

L

L

P

P

R

S

N

S

L

L

E

D

E

S

G

A

L

L

T

R

L

A

M

M

A

E

A

A

C

S

E

E

P

L

L

V

A

A

G

E

I

A

L

L

G

G

D

E

K

H

F

V

L

F

K

D

A

F

Y

F

F

L

A

R

L

N

K

K

E

R

T

T

E

E

Y

W

E

A

A

N

F

Y

F

R

R

S

V

H

I

V

S

T

S

P

W

Y

E

E

R

L

R

R

H

T

L

Y

L

L

K363 (≠ Q370) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 5 papers)
28% identity, 99% coverage: 2:442/445 of query aligns to 7:441/444 of P12425

query
sites
P12425

Q

E

D

D

I

I

E

E

D

K

F

L

L

V

K

K

Q

E

H

E

R

N

I

V

T

K

E

Y

I

I

E

R

A

L

I

Q

I

F

P

T

D

D

M

I

A

L

G

G

I

T

A

I

R

K

G

N

K

V

I

E

T

I

P

P

R

V

N

S

K

Q

F

L

T

G

S

K

G

A

E

-

S

-

M

-

R

-

L

L

P

D

Q

N

A

K

V

V

M

M

V

F

Q

D

T

G

V

S

T

S

G

I

E

E

Y

-

P

-

E

-

D

-

G

-

S
x
G

L

F

T

V

G
x
R

V

I

T

E

D

E

P

S

D

D

M

M

V

Y

C

L

V

Y

P

P

D

D

T

L

S

N

T

T

I

F

R

V

L

I

I

F

P

P

W

W

A

T

V

A

D

E

P

K

-

G

-

K

T

V

A

A

Q

R

V

F

I

I

H

C

D

D

C

I

V

Y

H

N

F

P

D

D

G

G

S

T

P

P

V

F

E

E

I

G

S

D

P

P

R

R

Y

N

V

N

L

L

R

K

R

R

V

I

L

L

E

K

L

E

Y

M

K

E

A

D

K

L

G

G

W

F

K

S

P

D

V

F

-

N

V

L

A

G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

-

F

-

K

V

L

D

D

M

E

N

K

K

G

D

E

P

P

D

T

L

L

P

E

L

L

R

N

P

D

P

K

V

G

G

G

R

Y

T

-

G

-

R

-

P

-

E

-

T

-

G

-

R

-

Q

-

S

-

Y

F

S

D

I

L

E

A

A

P

V

T

N

D

E

L

F

G

D

E

P

N

L

C

F

R

E

R

D

D

I

I

Y

V

E

L

Y

E

C

L

E

E

M

E

Q

M

N

G

L

F

D

E

I

I

D

E

T

A

L

S

I

H

H

H

E
|
E

V
|
V

G

A

A

P

A

G

Q

Q

M

H

E
|
E

I

I

N

D

F

F

M

K

H

Y

G

A

D

G

A

A

L

V

S

R

L

S

A

C

D

D

Q

D

V

I

F

Q

L

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

A

I

A

A

L

R

R

K

H

H

S

G

M

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

E

F

G

G

E

V

P

N

G
|
G

S

S

A

G

M

M

H
|
H

V

C

H

N

Q

L

S

S

I

L

V

F

D

-

E

-

E

K

T

N

G

G

R

V

N

N

L

A

F

F

T

F

S

D

P

E

E

N

T

A

G

D

G

L

A

Q

T

L

S

S

-

E

L

T

F

A

Y

K

N

H

Y

F

L

I

A

A

G

G

L

I

Q

V

K

K

Y

H

T

A

P

T

A

S

L

F

M

T

P

A

I

V

F

T

A

N

P

P

Y

T

I

V

N

N

S

S

Y

Y

R

K

R

R

L

L

S

V

R

P

F
x
G

M

Y

A
x
E

A

A

P
|
P

I

C

N

Y

V

V

Q

A

W

W

G

S

Y

A

D

Q

N

N

R

R

T

S

V

P

G

L

F

I

R

R

I

I

P

P

H

A

S

S

G

R

P

G

A

I

A

S

R

T

R

R

I

V

E
|
E

N

V

R

R

I

S

P

V

G

D

V

P

D

A

C

A

N

N

P

P

Y

Y

L

L

A

A

L

L

A

S

A

V

T

L

L

L

A

A

G

G

Y

L

L

D

G

G

M

I

T

K

Q

N

R

K

L

L

E

E

P

A

T

P

E

A

P

P

L

I

V

D

S

R

D

N

G

I

Y

Y

S

V

L

M

P

S

Y

K

Q

E

-

E

-

R

-

M

-

E

-

N

-

G

-

I

-

V

-

D

L

L

P

P

R

A

N

T

L

L

E

A

E

E

G

A

L

L

T

E

L

E

M

F

A

K

A

S

C

N

E

E

P

V

L

M

A

V

G

K

I

A

L

L

G

G

D

E

K

H

F

L

L

F

K

E

A

H

Y

F

F

I

A

E

L

A

K

K

E

E

T

I

E
|
E

Y

W

E

D

A

M

F

F

F

R

R

T

V

Q

I

V

S

H

S

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W

W

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E

R

R

R

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M

G59 (≠ S63) mutation to R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
R62 (≠ G66) Important for inhibition by glutamine; mutation to A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.
E132 (= E133) binding Mg(2+)
E134 (= E135) binding Mg(2+)
E189 (= E198) binding Mg(2+)
V190 (= V199) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E205) binding Mg(2+)
G241 (= G250) binding L-glutamate
H245 (= H254) binding Mg(2+)
G302 (≠ F312) mutation to E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
E304 (≠ A314) mutation to A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
P306 (= P316) mutation to H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
E333 (= E343) binding Mg(2+)
E424 (= E425) mutation to K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4s0rD Structure of gs-tnra complex (see paper)
28% identity, 99% coverage: 2:442/445 of query aligns to 10:444/447 of 4s0rD

query
sites
4s0rD

Q

E

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D

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-

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x
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-

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active site: D56 (≠ Q52), E135 (= E133), E137 (= E135), E192 (= E198), E199 (= E205), H248 (= H254), R319 (= R326), E336 (= E343), R338 (= R345)
binding glutamine: E137 (= E135), E192 (= E198), R301 (= R308), E307 (≠ A314)
binding magnesium ion: I66 (≠ V67), E135 (= E133), E135 (= E133), E199 (= E205), H248 (= H254), H248 (= H254), E336 (= E343), H419 (≠ A417)
binding : F63 (≠ L64), V64 (≠ T65), R65 (≠ G66), I66 (≠ V67), D161 (≠ S167), G241 (= G247), V242 (≠ E248), N243 (≠ P249), G305 (≠ F312), Y306 (≠ M313), Y376 (= Y383), I426 (≠ T424), M430 (≠ A428)

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
28% identity, 99% coverage: 2:442/445 of query aligns to 6:440/443 of 4lnkA

query
sites
4lnkA

Q

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S

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active site: D52 (≠ Q52), E131 (= E133), E133 (= E135), E188 (= E198), E195 (= E205), H244 (= H254), R315 (= R326), E332 (= E343), R334 (= R345)
binding adenosine-5'-diphosphate: K43 (≠ S39), M50 (= M50), F198 (= F208), Y200 (≠ H210), N246 (≠ H256), S248 (= S258), S324 (= S335), S328 (≠ A339), R330 (= R341)
binding glutamic acid: E133 (= E135), E188 (= E198), V189 (= V199), N239 (≠ P249), G240 (= G250), G242 (≠ A252), E303 (≠ A314)
binding magnesium ion: E131 (= E133), E188 (= E198), E195 (= E205), H244 (= H254), E332 (= E343)

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
28% identity, 99% coverage: 2:442/445 of query aligns to 6:440/443 of 4lniA

query
sites
4lniA

Q

E

D

D

I

I

E

E

D

K

F

L

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K

K

Q

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E

T

A

L

S

I

H

H

H

E
|
E

V

V

G

A

A

P

A

G

Q

Q

M

H

E
|
E

I

I

N
x
D

F

F

M

K

H
x
Y

G

A

D

G

A

A

L

V

S

R

L

S

A

C

D

D

Q

D

V

I

F

Q

L

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

A

I

A

A

L

R

R

K

H

H

S

G

M

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

E

F

G

G

E

V

P

N

G

G

S

S

A

G

M

M

H
|
H

V

C

H
x
N

Q

L

S
|
S

I

L

V

F

D

-

E

-

E

K

T

N

G

G

R

V

N

N

L

A

F

F

T

F

S

D

P

E

E

N

T

A

G

D

G

L

A

Q

T

L

S

S

-

E

L

T

F

A

Y

K

N

H

Y

F

L

I

A

A

G

G

L

I

Q

V

K

K

Y

H

T

A

P

T

A

S

L

F

M

T

P

A

I

V

F

T

A

N

P

P

Y

T

I

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

S

V

R

P

F

G

M

Y

A
x
E

A

A

P

P

I

C

N

Y

V

V

Q

A

W

W

G

S

Y

A

D

Q

N

N

R
|
R

T

S

V

P

G

L

F

I

R
|
R

I

I

P

P

H

A

S

S

G

R

P

G

A

I

A

S

R

T

R
|
R

I

V

E
|
E

N

V

R
|
R

I

S

P

V

G

D

V

P

D

A

C

A

N

N

P

P

Y

Y

L

L

A

A

L

L

A

S

A

V

T

L

L

L

A

A

G

G

Y

L

L

D

G

G

M

I

T

K

Q

N

R

K

L

L

E

E

P

A

T

P

E

A

P

P

L

I

V

D

S

R

D

N

G

I

Y

Y

S

V

L

M

P

S

Y

K

Q

E

-

E

-

R

-

M

-

E

-

N

-

G

-

I

-

V

-

D

L

L

P

P

R

A

N

T

L

L

E

A

E

E

G

A

L

L

T

E

L

E

M

F

A

K

A

S

C

N

E

E

P

V

L

M

A

V

G

K

I

A

L

L

G

G

D

E

K

H

F

L

L

F

K

E

A

H

Y

F

F

I

A

E

L

A

K

K

E

E

T

I

E

E

Y

W

E

D

A

M

F

F

F

R

R

T

V

Q

I

V

S

H

S

P

W

W

E

E

R

R

R

E

H

Q

L

Y

L

M

active site: D52 (≠ Q52), E131 (= E133), E133 (= E135), E188 (= E198), E195 (= E205), H244 (= H254), R315 (= R326), E332 (= E343), R334 (= R345)
binding adenosine-5'-diphosphate: E131 (= E133), E183 (≠ D193), D197 (≠ N207), Y200 (≠ H210), N246 (≠ H256), S248 (= S258), R320 (= R331), R330 (= R341)
binding magnesium ion: E131 (= E133), E131 (= E133), E133 (= E135), E188 (= E198), E195 (= E205), E195 (= E205), H244 (= H254), E332 (= E343)
binding l-methionine-s-sulfoximine phosphate: E133 (= E135), E188 (= E198), H244 (= H254), R297 (= R308), E303 (≠ A314), R315 (= R326), R334 (= R345)

7tenA Glutamine synthetase (see paper)
27% identity, 99% coverage: 2:442/445 of query aligns to 5:439/442 of 7tenA

query
sites
7tenA

Q

E

D

D

I

I

E

F

D

R

F

F

L

A

K

D

Q

E

H

Q

R

N

I

V

T

K

E

F

I

I

E

R

A

L

I

Q

I

F

P

T

D

D

M

I

A

L

G

G

I

I

A

I

R

K

G

-

K

-

I

-

T

-

P

-

R

-

N

-

K

-

F

-

T

-

S

-

G

-

E

-

S

N

M

V

R

E

L

I

P

P

Q

V

A

S

V

Q

M

L

V

K

Q

K

T

A

V

L

T

D

G

N

E

K

Y

I

P

M

E

F

D

D

G

G

S

S

-

S

-

I

-

E

-

G

L

F

T

V

G

R

V

I

T

E

D

E

P

S

D

D

M

M

V

Y

C

L

V

F

P

P

D

D

T

L

S

D

T

T

I

W

R

V

L

V

I

F

P

P

W

W

A

T

V

A

D

E

P

K

-

G

-

K

T

V

A

A

Q

R

V

M

I

I

H

C

D

D

C

I

V

Y

H

N

F

P

D

D

G

M

S

T

P

P

V

F

E

A

I

G

S

D

P

P

R

R

Y

A

V

N

L

L

R

K

R

R

V

V

L

L

E

K

L

E

Y

M

K

E

A

E

K

L

G

G

W

F

K

T

P

E

V

F

-

N

V

L

A
x
G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

-

F

-

K

V

L

D

D

M

E

N

N

K

R

D

R

P

P

D

T

L

L

P

E

L

L

R

N

P

D

P

S

V

G

G

G

R

-

T

-

G

-

R

-

P

-

E

-

T

-

G

-

R

-

Q

-

S

Y

Y

F

S

D

I

L

E

A

A

P

V

T

N

D

E

L

F

G

D

E

P

N

L

C

F

R

E

R

D

D

I

I

Y

V

E

L

Y

E

C

L

E

E

M

E

Q

M

N

G

L

F

D

E

I

I

D
x
E

T

A

L

S

I

H

H

H

E
|
E

V

V

G

A

A

P

A

G

Q

Q

M

H

E
|
E

I

I

N
x
D

F
|
F

M
x
K

H
x
Y

G

E

D

D

A

A

L

I

S

T

L

A

A

C

D

D

Q

S

V

I

F

Q

L

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

A

I

A

A

L

R

R

K

H

H

S

G

M

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

E

F

G

G

E

V

P
x
N

G
|
G

S

S

A

G

M

M

H
|
H

V

F

H
x
N

Q

M

S
|
S

I

L

V

F

D

N

E

E

E

K

T

-

G

G

R

N

N

A

L

F

F

F

T

D

S

E

P

S

E

G

T

E

G

L

G

E

A

L

T

S

S

Q

L

T

F

A

Y

Y

N

H

Y

F

L

L

A

A

G

G

L

M

Q

L

K

K

Y

H

T

A

P

R

A

G

L

Y

M

T

P

A

I

V

F

T

A

N

P

P

Y

T

I

I

N

N

S

S

Y

F

R

K

R
|
R

L

L

S

V

R

P

F

G

M

Y

A
x
E

A

A

P

P

I

C

N

Y

V

I

Q

A

W

W

G

S

Y

G

D

K

N

N

R
|
R

T

S

V

P

G

L

F

V

R
|
R

I

V

P

P

H

S

S

S

G

R

P

G

A

L

A
x
S

R

T

R
|
R

I

L

E

E

N

L

R
|
R

I

S

P

V

G

D

V

P

D

S

C

A

N

N

P

P

Y

Y

L

L

A

A

L

M

A

A

A

V

T

L

L

L

A

K

A

A

G

G

Y

L

L

S

G

G

M

I

T

K

Q

D

R

E

L

L

E

T

P

P

T

P

E

A

P

P

L

V

V

D

S

R

D

N

G

I

Y

Y

S

G

L

M

P

N

-

E

-

R

-

E

-

A

-

T

-

G

-

I

Y

Y

Q

D

L

L

P

P

R

E

N

S

L

L

E

G

E

H

G

A

L

L

T

I

L

E

M

L

A

E

A

K

C

N

E

E

P

I

L

I

A

K

G

D

I

G

L

L

G

G

D

E

K

H

F

I

L

F

K

E

A

H

Y

F

F

I

A

E

L

A

K

K

E

T

T

I

E

E

Y

C

E

D

A

M

F

F

F

R

R

T

V

A

I

V

S

H

S

P

W

W

E

E

R

R

R

E

H

Q

L

Y

L

L

binding adenosine-5'-diphosphate: G128 (≠ A131), E130 (= E133), E182 (≠ D193), D196 (≠ N207), F197 (= F208), K198 (≠ M209), Y199 (≠ H210), N245 (≠ H256), S247 (= S258), R319 (= R331), S327 (≠ A339), R329 (= R341)
binding l-methionine-s-sulfoximine phosphate: E130 (= E133), E132 (= E135), E187 (= E198), E194 (= E205), N238 (≠ P249), G239 (= G250), H243 (= H254), R296 (= R308), E302 (≠ A314), R314 (= R326), R333 (= R345)

7tf9S L. Monocytogenes gs(14)-q-glnr peptide (see paper)
27% identity, 99% coverage: 2:442/445 of query aligns to 6:440/443 of 7tf9S

query
sites
7tf9S

Q

E

D

D

I

I

E

F

D

R

F

F

L

A

K

D

Q

E

H

Q

R

N

I

V

T

K

E

F

I

I

E

R

A

L

I

Q

I

F

P

T

D

D

M

I

A

L

G

G

I

I

A

I

R

K

G

-

K

-

I

-

T

-

P

-

R

-

N

-

K

-

F

-

T

-

S

-

G

-

E

-

S

N

M

V

R

E

L

I

P

P

Q

V

A

S

V

Q

M

L

V

K

Q

K

T

A

V

L

T

D

G

N

E

K

Y

I

P

M

E

F

D

D

G

G

S

S

-

S

-

I

-

E

-

G

L
x
F

T
x
V

G

R

V

I

T

E

D

E

P

S

D

D

M

M

V

Y

C

L

V

F

P

P

D

D

T

L

S

D

T

T

I

W

R

V

L

V

I

F

P

P

W

W

A

T

V

A

D

E

P

K

-

G

-

K

T

V

A

A

Q

R

V

M

I

I

H

C

D

D

C

I

V

Y

H

N

F

P

D

D

G

M

S

T

P

P

V

F

E

A

I

G

S

D

P

P

R

R

Y

A

V

N

L

L

R

K

R

R

V

V

L

L

E

K

L

E

Y

M

K

E

A

E

K

L

G

G

W

F

K

T

P

E

V

F

-

N

V

L

A

G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

-

F

-

K

V

L

D

D

M

E

N

N

K

R

D

R

P

P

D

T

L

L

P

E

L

L

R

N

P

D

P

S

V

G

G

G

R

-

T

-

G

-

R

-

P

-

E

-

T

-

G

-

R

-

Q

-

S
x
Y

Y

F

S

D

I

L

E

A

A

P

V

T

N

D

E

L

F

G

D

E

P

N

L

C

F

R

E

R

D

D

I

I

Y

V

E

L

Y

E

C

L

E

E

M

E

Q

M

N

G

L

F

D

E

I

I

D

E

T

A

L

S

I

H

H

H

E
|
E

V

V

G

A

A

P

A

G

Q

Q

M

H

E
|
E

I

I

N

D

F

F

M

K

H

Y

G

E

D

D

A

A

L

I

S

T

L

A

A

C

D

D

Q

S

V

I

F

Q

L

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

A

I

A

A

L

R

R

K

H

H

S

G

M

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

E

F

G

G

E

V

P

N

G
|
G

S

S

A
x
G

M

M

H
|
H

V

F

H

N

Q

M

S

S

I

L

V

F

D

N

E

E

E

K

T

-

G

G

R

N

N

A

L

F

F

F

T

D

S

E

P

S

E

G

T

E

G

L

G

E

A

L

T

S

S

Q

L

T

F

A

Y

Y

N

H

Y

F

L

L

A

A

G

G

L

M

Q

L

K

K

Y

H

T

A

P

R

A

G

L

Y

M

T

P

A

I

V

F

T

A

N

P

P

Y

T

I

I

N

N

S

S

Y

F

R

K

R
|
R

L

L

S

V

R

P

F

G

M

Y

A
x
E

A

A

P

P

I

C

N

Y

V

I

Q

A

W

W

G

S

Y

G

D

K

N

N

R

R

T

S

V

P

G

L

F

V

R

R

I

V

P

P

H

S

S

S

G

R

P

G

A

L

A

S

R

T

R

R

I

L

E
|
E

N

L

R

R

I

S

P

V

G

D

V

P

D

S

C

A

N

N

P

P

Y

Y

L

L

A

A

L

M

A

A

A

V

T

L

L

L

A

K

A

A

G

G

Y

L

L

S

G

G

M

I

T

K

Q

D

R

E

L

L

E

T

P

P

T

P

E

A

P

P

L

V

V

D

S

R

D

N

G

I

Y

Y

S

G

L

M

P

N

-

E

-

R

-

E

-

A

-

T

-

G

-

I

Y

Y

Q

D

L

L

P

P

R

E

N

S

L

L

E

G

E

H

G

A

L

L

T

I

L

E

M

L

A

E

A

K

C

N

E

E

P

I

L

I

A

K

G

D

I

G

L

L

G

G

D

E

K

H

F

I

L

F

K

E

A

H

Y

F

F

I

A
x
E

L

A

K

K

E

T

T
x
I

E

E

Y

C

E

D

A
x
M

F

F

F

R

R

T

V

A

I

V

S

H

S

P

W

W

E

E

R

R

R

E

H

Q

L

Y

L

L

binding glutamine: E133 (= E135), Y155 (≠ S165), E188 (= E198), G240 (= G250), G242 (≠ A252), R297 (= R308), E303 (≠ A314)
binding magnesium ion: E131 (= E133), E133 (= E135), E188 (= E198), E195 (= E205), H244 (= H254), E332 (= E343)
binding : F59 (≠ L64), V60 (≠ T65), E418 (≠ A420), I422 (≠ T424), M426 (≠ A428)

Query Sequence

>WP_011886233.1 NCBI__GCF_000016205.1:WP_011886233.1
MQDIEDFLKQHRITEIEAIIPDMAGIARGKITPRNKFTSGESMRLPQAVMVQTVTGEYPE
DGSLTGVTDPDMVCVPDTSTIRLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLEL
YKAKGWKPVVAPELEFYLVDMNKDPDLPLRPPVGRTGRPETGRQSYSIEAVNEFDPLFED
IYEYCEMQNLDIDTLIHEVGAAQMEINFMHGDALSLADQVFLFKRTVREAALRHSMYATF
MAKPMEGEPGSAMHVHQSIVDEETGRNLFTSPETGGATSLFYNYLAGLQKYTPALMPIFA
PYINSYRRLSRFMAAPINVQWGYDNRTVGFRIPHSGPAARRIENRIPGVDCNPYLALAAT
LAAGYLGMTQRLEPTEPLVSDGYSLPYQLPRNLEEGLTLMAACEPLAGILGDKFLKAYFA
LKETEYEAFFRVISSWERRHLLLHV

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SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory