SitesBLAST
Comparing WP_011911296.1 NCBI__GCF_000013785.1:WP_011911296.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q9A3Q9 Omega-aminotransferase; Beta-alanine--pyruvate aminotransferase; EC 2.6.1.-; EC 2.6.1.18 from Caulobacter vibrioides (strain ATCC 19089 / CIP 103742 / CB 15) (Caulobacter crescentus) (see paper)
61% identity, 97% coverage: 4:431/439 of query aligns to 3:431/439 of Q9A3Q9
- V227 (= V227) mutation to G: Decreases activity toward 3-aminobutanoate by 2-fold. Increases activity toward the aromatic beta-amino acid 3-amino-3-phenylpropanoate by 2-fold.
- R260 (= R260) mutation to L: Decreases activity toward 3-aminobutanoate by 30-fold.
- N285 (= N285) mutation to A: Decreases activity toward 3-aminobutanoate by 4-fold. Increases activity toward the aromatic beta-amino acid 3-amino-3-phenylpropanoate by 3-fold.
4uhmA Characterization of a novel transaminase from pseudomonas sp. Strain aac (see paper)
52% identity, 97% coverage: 13:436/439 of query aligns to 8:432/435 of 4uhmA
- active site: F11 (= F16), Y140 (= Y145), E213 (= E219), D246 (= D252), I249 (= I255), K275 (= K281), Q408 (≠ A412)
- binding magnesium ion: A91 (= A96), D99 (≠ N104)
- binding pyridoxal-5'-phosphate: G107 (= G112), S108 (= S113), Y140 (= Y145), H141 (= H146), G142 (= G147), E213 (= E219), D246 (= D252), V248 (= V254), I249 (= I255), K275 (= K281)
Q9I700 Beta-alanine--pyruvate aminotransferase; Beta-A--Py AT; Beta-alanine--pyruvate transaminase; Omega-amino acid aminotransferase; Omega-amino acid AT; Omega-amino acid--pyruvate aminotransferase; Omega-APT; EC 2.6.1.18 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
52% identity, 97% coverage: 13:436/439 of query aligns to 21:445/448 of Q9I700
- W61 (= W53) binding substrate
- T327 (= T318) binding pyridoxal 5'-phosphate
- R414 (= R405) binding substrate
- Q421 (≠ A412) binding substrate
4b98A The structure of the omega aminotransferase from pseudomonas aeruginosa (see paper)
52% identity, 97% coverage: 13:436/439 of query aligns to 14:438/441 of 4b98A
- active site: F17 (= F16), Y146 (= Y145), E219 (= E219), D252 (= D252), I255 (= I255), K281 (= K281), Q414 (≠ A412)
- binding pyridoxal-5'-phosphate: G233 (= G233), Q236 (= Q236), F270 (≠ W270), G271 (= G271)
- binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: L53 (= L52), W54 (= W53), F82 (= F81), S112 (= S111), G113 (= G112), S114 (= S113), Y146 (= Y145), H147 (= H146), G148 (= G147), E219 (= E219), S224 (= S224), D252 (= D252), V254 (= V254), I255 (= I255), K281 (= K281), Y319 (= Y317), T320 (= T318), R407 (= R405), Q414 (≠ A412)
3a8uX Crystal structure of omega-amino acid:pyruvate aminotransferase
50% identity, 97% coverage: 13:437/439 of query aligns to 13:439/441 of 3a8uX
- active site: Y145 (= Y145), D252 (= D252), K281 (= K281), Q414 (≠ A412)
- binding pyridoxal-5'-phosphate: S111 (= S111), G112 (= G112), S113 (= S113), Y145 (= Y145), H146 (= H146), G147 (= G147), E219 (= E219), D252 (= D252), V254 (= V254), I255 (= I255), K281 (= K281)
6gwiB The crystal structure of halomonas elongata amino-transferase (see paper)
40% identity, 98% coverage: 2:430/439 of query aligns to 3:437/450 of 6gwiB
- active site: F18 (= F16), Y149 (= Y145), D255 (= D252), K284 (= K281)
- binding pyridoxal-5'-phosphate: S115 (= S111), G116 (= G112), S117 (= S113), Y149 (= Y145), H150 (= H146), G151 (= G147), E222 (= E219), D255 (= D252), V257 (= V254), I258 (= I255), K284 (= K281)
7qx3A Structure of the transaminase tr2e2 with eos (see paper)
38% identity, 92% coverage: 27:429/439 of query aligns to 2:408/422 of 7qx3A
8wqjA Crystal structure of transaminase from shimia marina
37% identity, 95% coverage: 11:429/439 of query aligns to 19:441/472 of 8wqjA
- binding pyridoxal-5'-phosphate: S121 (= S111), G122 (= G112), S123 (= S113), Y155 (= Y145), H156 (= H146), G157 (= G147), E228 (= E219), D261 (= D252), V263 (= V254), I264 (= I255), K290 (= K281), Y321 (= Y317), T322 (= T318)
3i5tA Crystal structure of aminotransferase prk07036 from rhodobacter sphaeroides kd131
38% identity, 97% coverage: 10:437/439 of query aligns to 8:436/444 of 3i5tA
- active site: A14 (≠ F16), Y144 (= Y145), E216 (= E219), D249 (= D252), V252 (≠ I255), K279 (= K281), V411 (≠ A412)
- binding pyridoxal-5'-phosphate: G111 (= G112), S112 (= S113), Y144 (= Y145), H145 (= H146), E216 (= E219), D249 (= D252), V251 (= V254), K279 (= K281), Y315 (= Y317), T316 (= T318)
7ypmA Crystal structure of transaminase cc1012 complexed with plp and l- alanine (see paper)
38% identity, 97% coverage: 14:438/439 of query aligns to 15:447/454 of 7ypmA
- binding alanine: W57 (= W53), Y150 (= Y145)
- binding pyridoxal-5'-phosphate: S116 (= S111), G117 (= G112), S118 (= S113), Y150 (= Y145), G152 (= G147), E223 (= E219), D256 (= D252), V258 (= V254), I259 (= I255), K285 (= K281)
6io1B Crystal structure of a novel thermostable (s)-enantioselective omega- transaminase from thermomicrobium roseum (see paper)
39% identity, 96% coverage: 11:432/439 of query aligns to 15:440/448 of 6io1B