SitesBLAST
Comparing WP_011911348.1 NCBI__GCF_000013785.1:WP_011911348.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6iunB Crystal structure of enoyl-coa hydratase (ech) from ralstonia eutropha h16 in complex with NAD
51% identity, 97% coverage: 13:695/701 of query aligns to 10:691/692 of 6iunB
- active site: A60 (= A63), F65 (= F68), E73 (≠ A74), H77 (≠ P78), G101 (= G102), E104 (= E105), E124 (= E125), G132 (= G133), K248 (= K252), S407 (= S411), H428 (= H432), E440 (= E444), N478 (= N482)
- binding nicotinamide-adenine-dinucleotide: G300 (= G304), T301 (= T305), M302 (= M306), E321 (= E325), T322 (≠ I326), Y365 (= Y369), A377 (= A381), V378 (= V382), E380 (= E384), V384 (= V388), V388 (= V392), N405 (= N409), S407 (= S411)
Q08426 Peroxisomal bifunctional enzyme; PBE; PBFE; L-bifunctional protein; LBP; Multifunctional enzyme 1; MFE1; EC 4.2.1.17; EC 5.3.3.8; EC 1.1.1.35 from Homo sapiens (Human) (see 5 papers)
41% identity, 100% coverage: 1:699/701 of query aligns to 1:714/723 of Q08426
- E3 (≠ D3) to K: in FRTS3; the mutant is mistargeted to mitochondria; results in impaired mitochondrial oxidative phosphorylation and defects in the transport of fluids across the epithelium of renal proximal tubular cells; dbSNP:rs398124646
- V40 (≠ D42) to G: in dbSNP:rs1062551
- I41 (≠ E43) to R: in dbSNP:rs1062552
- T75 (≠ S76) to I: in dbSNP:rs1062553
- K165 (≠ E166) modified: N6-acetyllysine; alternate; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-171; Q-346 and Q-584.
- K171 (≠ E172) modified: N6-acetyllysine; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-346 and Q-584.
- A274 (= A276) to T: in dbSNP:rs2302819
- A325 (≠ L323) to G: in dbSNP:rs1062555
- K346 (≠ T341) modified: N6-acetyllysine; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-584.
- K584 (vs. gap) modified: N6-acetyllysine; alternate; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-346.
- K598 (≠ P586) to T: in dbSNP:rs1042437
- T606 (≠ E594) to P: in dbSNP:rs1042438
5omoA Crystal structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with with 3s-hydroxy-decanoyl-coa and 3-keto- decanoyl-coa
40% identity, 100% coverage: 1:699/701 of query aligns to 6:718/725 of 5omoA
- active site: A66 (= A63), F71 (= F68), G81 (≠ P78), G105 (= G102), E108 (= E105), P127 (= P124), E128 (= E125), P135 (= P132), G136 (= G133), K254 (= K252), S415 (= S411), H436 (= H432), E448 (= E444), N486 (= N482)
- binding (s)-3-hydroxydecanoyl-coa: P25 (= P22), V26 (= V23), A28 (= A25), P31 (≠ H28), A64 (= A61), A66 (= A63), D67 (= D64), I68 (= I65), L103 (= L100), G105 (= G102), E108 (= E105), P127 (= P124), E128 (= E125), Y161 (≠ P158), F260 (= F258), K280 (≠ R278)
- binding 3-keto-decanoyl-coa: S415 (= S411), N486 (= N482), K519 (≠ A515), M520 (= M516), V525 (≠ M521), Y658 (= Y642)
5mgbA Crystal structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with acetoacetyl-coa and NAD (see paper)
40% identity, 100% coverage: 1:699/701 of query aligns to 6:718/725 of 5mgbA
- active site: A66 (= A63), F71 (= F68), G81 (≠ P78), G105 (= G102), E108 (= E105), P127 (= P124), E128 (= E125), P135 (= P132), G136 (= G133), K254 (= K252), S415 (= S411), H436 (= H432), E448 (= E444), N486 (= N482)
- binding acetoacetyl-coenzyme a: P25 (= P22), V26 (= V23), A64 (= A61), G65 (= G62), A66 (= A63), D67 (= D64), I68 (= I65), G105 (= G102), E128 (= E125), Y161 (≠ P158)
- binding nicotinamide-adenine-dinucleotide: L307 (≠ I301), G308 (= G302), G310 (= G304), T311 (= T305), M312 (= M306), E331 (= E325), S332 (≠ I326), Q336 (≠ A330), V386 (= V382), F387 (= F383), E388 (= E384), N413 (= N409), S415 (= S411), H436 (= H432)
3zwcA Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 3s-hydroxy-decanoyl-coa (see paper)
40% identity, 100% coverage: 1:699/701 of query aligns to 6:718/725 of 3zwcA
- active site: A66 (= A63), F71 (= F68), G81 (≠ P78), G105 (= G102), E108 (= E105), P127 (= P124), E128 (= E125), P135 (= P132), G136 (= G133), K254 (= K252), S415 (= S411), H436 (= H432), E448 (= E444), N486 (= N482)
- binding (s)-3-hydroxydecanoyl-coa: V26 (= V23), A64 (= A61), G65 (= G62), A66 (= A63), D67 (= D64), I68 (= I65), G77 (≠ A74), L78 (≠ P75), L80 (= L77), V101 (≠ S98), G104 (= G101), G105 (= G102), E108 (= E105), E128 (= E125), F260 (= F258)
- binding nicotinamide-adenine-dinucleotide: G308 (= G302), G310 (= G304), T311 (= T305), M312 (= M306), E331 (= E325), Q336 (≠ A330), A385 (= A381), V386 (= V382), F387 (= F383), E388 (= E384), K393 (= K389), N413 (= N409), S415 (= S411), H436 (= H432)
2x58A The crystal structure of mfe1 liganded with coa (see paper)
40% identity, 100% coverage: 1:699/701 of query aligns to 6:718/725 of 2x58A
- active site: A66 (= A63), F71 (= F68), G81 (≠ P78), G105 (= G102), E108 (= E105), P127 (= P124), E128 (= E125), P135 (= P132), G136 (= G133), K254 (= K252), S415 (= S411), H436 (= H432), E448 (= E444), N486 (= N482)
- binding adenosine-5'-diphosphate: G310 (= G304), T311 (= T305), M312 (= M306), E331 (= E325), S332 (≠ I326), Q336 (≠ A330), V386 (= V382), L392 (≠ V388)
- binding coenzyme a: V26 (= V23), A28 (= A25), A64 (= A61), A66 (= A63), D67 (= D64), I68 (= I65), E128 (= E125)
3zw9A Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with (2s,3s)-3-hydroxy-2-methylbutanoyl-coa (see paper)
40% identity, 100% coverage: 1:699/701 of query aligns to 4:716/723 of 3zw9A