SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011911575.1 NCBI__GCF_000013785.1:WP_011911575.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

7bgnA Crystal structure of mthisn2-amp complex, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
44% identity, 81% coverage: 2:107/131 of query aligns to 4:109/204 of 7bgnA

query
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7bgnA

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binding adenosine monophosphate: W60 (= W58), K62 (= K60), G63 (= G61), T65 (≠ E63), S66 (= S64), T94 (≠ A93), H96 (= H95)
binding zinc ion: D78 (= D76), D80 (= D78), D82 (= D80), C95 (= C94), C102 (= C101)

Sites not aligning to the query:

binding adenosine monophosphate: 121, 128, 135, 136, 137, 180, 203, 204
binding zinc ion: 154, 174, 177

7bgmA Crystal structure of mthisn2, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
44% identity, 81% coverage: 2:107/131 of query aligns to 6:111/213 of 7bgmA

query
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7bgmA

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binding zinc ion: D80 (= D76), D82 (= D78), D84 (= D80), C97 (= C94), C104 (= C101)

Sites not aligning to the query:

binding zinc ion: 163, 183, 186

6j2lA Crystal structure of bi-functional enzyme (see paper)
40% identity, 74% coverage: 6:102/131 of query aligns to 8:104/200 of 6j2lA

query
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6j2lA

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binding zinc ion: D79 (= D76), D81 (= D78), D83 (= D80), C96 (= C94), C103 (= C101)

Sites not aligning to the query:

binding zinc ion: 152, 171, 174

6j2lB Crystal structure of bi-functional enzyme (see paper)
38% identity, 68% coverage: 6:94/131 of query aligns to 7:96/185 of 6j2lB

query
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6j2lB

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binding zinc ion: D78 (= D76), D80 (= D78), D82 (= D80)

Sites not aligning to the query:

binding magnesium ion: 137, 140, 156, 159

Query Sequence

>WP_011911575.1 NCBI__GCF_000013785.1:WP_011911575.1
MNWLDEINWNADGLVPAIAQDHKTGRVLMMAWMNREALQLTAQENRAIYWSRSRGKLWRK
GEESGHVQQLHELRLDCDADVIILMVEQIGGIACHTGRESCFYRVFENGAWKVVEPVLKD
PHAIYAEHKHE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory