SitesBLAST
Comparing WP_011911635.1 NCBI__GCF_000013785.1:WP_011911635.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3pm9A Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
38% identity, 99% coverage: 4:461/464 of query aligns to 4:464/465 of 3pm9A
- active site: A149 (= A149), L159 (≠ M159)
- binding flavin-adenine dinucleotide: P69 (= P69), Q70 (≠ S70), G71 (= G71), G72 (= G72), N73 (≠ R73), T74 (= T74), G75 (= G75), L76 (= L76), G79 (≠ A79), Q80 (≠ A80), L91 (≠ F91), L133 (≠ F133), G134 (≠ A134), A135 (≠ S135), C139 (≠ S139), T140 (≠ Q140), G142 (= G142), G143 (= G143), S146 (≠ G146), T147 (= T147), A149 (= A149), G150 (= G150), E200 (= E200), G201 (= G201), I205 (≠ F205), I206 (≠ V206), E423 (= E420)
6lpnB Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
35% identity, 97% coverage: 14:464/464 of query aligns to 21:467/467 of 6lpnB
- binding flavin-adenine dinucleotide: W40 (= W33), P76 (= P69), G78 (= G71), G79 (= G72), N80 (≠ R73), T81 (= T74), G82 (= G75), M83 (≠ L76), G86 (≠ A79), S87 (≠ A80), L140 (≠ F133), A142 (≠ S135), C146 (≠ S139), H147 (≠ Q140), G150 (= G143), N151 (= N144), A153 (≠ G146), T154 (= T147), G208 (= G201), I212 (≠ F205), I213 (≠ V206), E423 (= E420), N460 (= N457)
6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
35% identity, 97% coverage: 14:464/464 of query aligns to 20:466/466 of 6lpxA
- binding 2-oxoglutaric acid: R333 (= R325), T337 (≠ S329), K348 (= K339), Y379 (≠ F372), H381 (= H374), H388 (= H381), H423 (= H421)
- binding flavin-adenine dinucleotide: W39 (= W33), P75 (= P69), Q76 (≠ S70), G77 (= G71), G78 (= G72), N79 (≠ R73), T80 (= T74), G81 (= G75), M82 (≠ L76), G85 (≠ A79), S86 (≠ A80), L139 (≠ F133), G140 (≠ A134), A141 (≠ S135), C145 (≠ S139), G149 (= G143), N150 (= N144), A152 (≠ G146), T153 (= T147), G157 (= G151), G207 (= G201), I212 (≠ V206), E422 (= E420), N459 (= N457)
- binding zinc ion: H381 (= H374), H388 (= H381), E422 (= E420)
6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
35% identity, 97% coverage: 14:464/464 of query aligns to 20:466/466 of 6lpuA
- binding flavin-adenine dinucleotide: W39 (= W33), P75 (= P69), G77 (= G71), G78 (= G72), N79 (≠ R73), T80 (= T74), G81 (= G75), G85 (≠ A79), S86 (≠ A80), L139 (≠ F133), G140 (≠ A134), A141 (≠ S135), C145 (≠ S139), H146 (≠ Q140), G148 (= G142), G149 (= G143), N150 (= N144), A152 (≠ G146), T153 (= T147), A155 (= A149), E206 (= E200), G207 (= G201), I211 (≠ F205), I212 (≠ V206), E422 (= E420), N459 (= N457)
- binding (2s)-2-hydroxypentanedioic acid: R333 (= R325), T337 (≠ S329), K348 (= K339), Y379 (≠ F372), H381 (= H374), H388 (= H381), H423 (= H421)
- binding zinc ion: H381 (= H374), H388 (= H381), E422 (= E420)
6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal) (see paper)
35% identity, 97% coverage: 14:464/464 of query aligns to 20:466/466 of 6lpqA
- binding flavin-adenine dinucleotide: W39 (= W33), P75 (= P69), G77 (= G71), G78 (= G72), N79 (≠ R73), T80 (= T74), G81 (= G75), G85 (≠ A79), S86 (≠ A80), L139 (≠ F133), G140 (≠ A134), A141 (≠ S135), C145 (≠ S139), H146 (≠ Q140), G149 (= G143), N150 (= N144), A152 (≠ G146), T153 (= T147), A155 (= A149), G157 (= G151), E206 (= E200), G207 (= G201), I211 (≠ F205), I212 (≠ V206), E422 (= E420), N459 (= N457)
- binding d-malate: M82 (≠ L76), R333 (= R325), T337 (≠ S329), K348 (= K339), Y379 (≠ F372), H381 (= H374), H388 (= H381), E422 (= E420), H423 (= H421)
- binding zinc ion: H381 (= H374), H388 (= H381), E422 (= E420)
6lppA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-2-hydroxyglutarate (d-2-hg) (see paper)
35% identity, 97% coverage: 14:464/464 of query aligns to 20:466/466 of 6lppA
- binding (2R)-2-hydroxypentanedioic acid: R333 (= R325), T337 (≠ S329), K348 (= K339), Y379 (≠ F372), H381 (= H374), H388 (= H381), N390 (= N383), H423 (= H421)
- binding flavin-adenine dinucleotide: W39 (= W33), P75 (= P69), G77 (= G71), G78 (= G72), N79 (≠ R73), T80 (= T74), G81 (= G75), M82 (≠ L76), G85 (≠ A79), S86 (≠ A80), L139 (≠ F133), G140 (≠ A134), A141 (≠ S135), C145 (≠ S139), G149 (= G143), N150 (= N144), A152 (≠ G146), T153 (= T147), A155 (= A149), G157 (= G151), G207 (= G201), I212 (≠ V206), E422 (= E420), H423 (= H421)
- binding zinc ion: H381 (= H374), H388 (= H381), E422 (= E420)
Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
35% identity, 97% coverage: 14:464/464 of query aligns to 73:519/521 of Q8N465
- S109 (≠ I50) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
- N127 (≠ V68) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
- G131 (= G72) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
- I147 (≠ V88) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
- M153 (= M94) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
- C172 (≠ V113) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
- P189 (= P130) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
- A205 (≠ G146) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
- A231 (≠ G172) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
- G233 (= G174) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734
- D375 (≠ S314) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
- R386 (= R325) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- T390 (≠ S329) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Significantly reduced catalytic activity.
- V399 (≠ P337) to M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
- K401 (= K339) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- R419 (≠ D357) to H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
- A426 (≠ Y364) to T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
- H434 (= H374) binding Zn(2+); mutation to A: Loss of catalytic activity.
- G436 (= G376) to V: slight reduction in catalytic activity
- N439 (= N379) to D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
- H441 (= H381) binding Zn(2+); mutation to A: Loss of catalytic activity.
- N443 (= N383) binding (R)-2-hydroxyglutarate; mutation to A: Significantly reduced catalytic activity.
- V444 (≠ I384) to A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
- A446 (≠ K386) to V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
- E475 (= E420) binding Zn(2+); mutation to A: Loss of catalytic activity.
- H476 (= H421) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- G477 (= G422) to R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640
Sites not aligning to the query:
- 15 R → G: in dbSNP:rs4675887
- 169:521 natural variant: Missing (in D2HGA1; uncertain significance)
- 400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)
P39976 D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3; D-2HG--pyruvate transhydrogenase DLD3; (R)-2-hydroxyglutarate--pyruvate transhydrogenase; D-lactate dehydrogenase [cytochrome] 3; D-lactate ferricytochrome C oxidoreductase; D-LCR; EC 1.1.99.40; EC 1.1.2.4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
33% identity, 94% coverage: 24:460/464 of query aligns to 51:494/496 of P39976
Sites not aligning to the query:
- 17 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
29% identity, 98% coverage: 7:461/464 of query aligns to 11:463/467 of 7qh2C