SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011913061.1 NCBI__GCF_000013785.1:WP_011913061.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4n5fA Crystal structure of a putative acyl-coa dehydrogenase with bound fadh2 from burkholderia cenocepacia j2315
63% identity, 99% coverage: 2:375/379 of query aligns to 5:378/378 of 4n5fA

query
sites
4n5fA

L

L

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Y

N

T

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E

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D

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active site: L126 (= L123), T127 (= T124), G243 (= G240), E364 (= E361), R376 (= R373)
binding dihydroflavine-adenine dinucleotide: L126 (= L123), T127 (= T124), G132 (= G129), S133 (= S130), F157 (= F154), T159 (= T156), T210 (= T207), Y363 (= Y360), T366 (= T363), E368 (≠ D365), M372 (≠ L369)

4m9aB Crystal structure of acyl-coa dehydrogenase from burkholderia thailandensis e264
62% identity, 99% coverage: 2:375/379 of query aligns to 3:376/376 of 4m9aB

query
sites
4m9aB

L

L

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E

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active site: L124 (= L123), T125 (= T124), G241 (= G240), E362 (= E361), R374 (= R373)
binding dihydroflavine-adenine dinucleotide: F122 (= F121), T125 (= T124), G130 (= G129), S131 (= S130), F155 (= F154), T157 (= T156), T208 (= T207), Y361 (= Y360), T364 (= T363), E366 (≠ D365), M370 (≠ L369)

5lnxD Crystal structure of mmgc, an acyl-coa dehydrogenase from bacillus subtilis.
49% identity, 98% coverage: 5:377/379 of query aligns to 4:374/374 of 5lnxD

query
sites
5lnxD

E

Q

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active site: L122 (= L123), T123 (= T124), G239 (= G240), E358 (= E361), K370 (≠ R373)
binding flavin-adenine dinucleotide: L122 (= L123), T123 (= T124), G128 (= G129), S129 (= S130), F153 (= F154), T155 (= T156), R265 (= R266), Q267 (≠ T268), F268 (= F269), I272 (= I273), N275 (≠ H276), I278 (≠ V279), Q331 (= Q334), I332 (≠ T335), G335 (= G338), Y357 (= Y360), T360 (= T363), E362 (≠ D365)

4l1fA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
47% identity, 98% coverage: 4:375/379 of query aligns to 6:377/380 of 4l1fA

query
sites
4l1fA

N

T

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E

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x
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E

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G

T

I

I

T

M

L

S

S

V

A

H

N

N

V

S

S

V

L

G

C

C

A

V

T

P

P

I

I

L

Y

R

M

F

F

G

G

N

T

D

E

Q

E

Q

Q

K

K

R

Q

D

K

F

Y

L

L

G

A

P

P

L

I

A

A

R

E

G

G

E

T

Q

H

I

V

G

G

A

A

F
|
F

A

G

L
|
L

T
|
T

E

E

P

P

Q

S

A

A

G
|
G

S
x
T

D

D

A

A

S

S

S

A

L

Q

R

Q

T

T

R

T

A

A

R

V

R

L

D

K

G

G

D

D

H

K

Y

Y

V

I

L

L

N

N

G

G

A

S

K

K

Q

I

F
|
F

I
|
I

T
|
T

S

N

G

G

K

K

H

E

A

A

G

D

T

T

V

Y

I

V

V

V

F

F

A

A

V

M

T

T

D

D

P

K

D

S

A

Q

G

G

K

V

R
x
H

G

G

I

I

S

S

A

A

F

F

I

I

V

L

P

E

T

K

D

G

S

M

P

P

G

G

Y

F

Q

R

V

F

V

G

R

K

V

I

E

E

D

D

K

K

L

M

G

G

Q

G

H

H

A

T

S

S

D

I

T

T

C

A

Q

E

I

L

A

I

F

F

E

E

D

D

L

C

R

E

V

V

P

P

V

K

A

E

N

N

R

L

L

L

G

G

E

K

E

E

G

G

E

E

G

G

Y
x
F

R

K

I

I

A

A

L
x
M

A
x
E

N

T

L
|
L

E
x
D

G

G

G
|
G

R
|
R

I

I

G

G

I

V

A

A

A

A

Q

Q

A

A

V

L

G

G

M

I

A

A

R

E

A

G

A

A

F

L

E

A

A

A

A

A

R

V

D

K

Y

Y

A

S

G

K

E

E

R
|
R

E

E

T
x
Q

F
|
F

G

G

K

R

P

S

I
|
I

I

S

E

K

H
x
F

Q

Q

A

A

V
x
L

A

Q

F

F

R

M

L

M

A

A

D

D

M

M

A

A

T

T

Q

K

I

I

A

E

V

A

A

A

R

R

Q

Y

M

L

V

V

H

Y

H

H

A

A

A

A

A

M

L

L

R

K

E

N

A

E

G

G

R

K

P

P

A

Y

L

S

V

E

E

A

A

A

S

A

M

M

A

A

K

K

L

C

F

F

A

A

S

S

E

D

M

V

A

A

E

M

R

E

V

V

C

T

S

T

A

D

A

A

I

V

Q
|
Q

T
x
I

L

F

G

G

G
|
G

Y

Y

G

G

Y

Y

L

T

S

V

D

D

F

Y

P

P

V

A

E

E

R

R

I

Y

Y

M

R

R

D

N

V

A

R

K

V
x
I

C

T

Q

Q

I

I

Y
|
Y

E
|
E

G
|
G

T
|
T

S

N

D
x
Q

I

V

Q

M

R

R

L

I

V

V

I

T

S

S

R
|
R

N

A

L

L

active site: L125 (= L123), T126 (= T124), G242 (= G240), E363 (= E361), R375 (= R373)
binding coenzyme a persulfide: T132 (≠ S130), H179 (≠ R177), F232 (≠ Y230), M236 (≠ L234), E237 (≠ A235), L239 (= L237), D240 (≠ E238), R243 (= R241), Y362 (= Y360), E363 (= E361), G364 (= G362), R375 (= R373)
binding flavin-adenine dinucleotide: F123 (= F121), L125 (= L123), T126 (= T124), G131 (= G129), T132 (≠ S130), F156 (= F154), I157 (= I155), T158 (= T156), R268 (= R266), Q270 (≠ T268), F271 (= F269), I275 (= I273), F278 (≠ H276), L281 (≠ V279), Q336 (= Q334), I337 (≠ T335), G340 (= G338), I358 (≠ V356), Y362 (= Y360), T365 (= T363), Q367 (≠ D365)
binding 1,3-propandiol: Q10 (≠ C8)

Sites not aligning to the query:

binding 1,3-propandiol: 5

5ol2F The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
46% identity, 97% coverage: 10:375/379 of query aligns to 11:376/378 of 5ol2F

query
sites
5ol2F

I

L

A

K

E

E

M

L

A

Y

R

V

Q

S

F

F

A

A

R

E

E

N

R

E

L

V

K

K

P

P

F

L

A

A

E

T

Q
x
E

W

L

S

D

R

E

E
|
E

H

E

R
|
R

Y

F

P

P

A

Y

E

E

A

T

I

V

G

E

E

K

M

M

A

A

A

K

L

A

G

G

F

M

F

M

G

G

M

I

L

P

V

Y

P

P

E

K

Q

E

W

Y

G

G

G

G

S

E

D

G

T

G

G

D

Y

T

L

V

A

G

Y

Y

A

I

M

M

A

A

L

V

E

E

E

E

I

L

A

S

A

R

G

V

D

C

G

G

A

T

C

T

S

G

T

V

I

I

M

L

S

S

V

A

H

H

N

T

S

S

V

L

G

G

C

S

V

W

P

P

I

I

L

Y

R

Q

F

Y

G

G

N

N

D

E

Q

E

Q

Q

K

K

R

Q

D

K

F

F

L

L

G

R

P

P

L

L

A

A

R

S

G

G

E

E

Q

K

I

L

G

G

A

A

F
|
F

A

G

L
|
L

T
|
T

E

E

P
|
P

Q

N

A

A

G

G

S
x
T

D

D

A

A

S

S

S

G

L

Q

R

Q

T

T

R

T

A

A

R

V

R

L

D

D

G

G

D

D

H

E

Y

Y

V

I

L

L

N

N

G

G

A

S

K

K

Q

I

F
|
F

I
|
I

T
|
T

S

N

G

A

K

I

H

A

A

G

G

D

T

I

V

Y

I

V

V

V

F

M

A

A

V

M

T

T

D

D

P

K

D

S

A

K

G

G

K

N

R

K

G

G

I

I

S

S

A

A

F

F

I

I

V

V

P

E

T

K

D

G

S

T

P

P

G

G

Y

F

Q

S

V

F

V

G

R

V

V

K

E
|
E

D

K

K

K

L

M

G

G

Q

I

H

R

A

G

S

S

D

A

T

T

C

S

Q

E

I

L

A

I

F

F

E

E

D

D

L

C

R

R

V

I

P

P

V

K

A

E

N

N

R

L

L

L

G

G

E

K

E

E

G

G

E

Q

G

G

Y

F

R

K

I

I

A

A

L

M

A

S

N

T

L
|
L

E

D

G

G

G
|
G

R
|
R

I

I

G

G

I

I

A

A

A

A

Q

Q

A

A

V

L

G

G

M

L

A

A

R

Q

A

G

A

A

F

L

E

D

A

E

A

T

R

V

D

K

Y

Y

A

V

G

K

E

E

R
|
R

E

V

T

Q

F
|
F

G

G

K

R

P

P

I
x
L

I

S

E

K

H
x
F

Q

Q

A

N

V

T

A

Q

F

F

R

Q

L

L

A

A

D

D

M

M

A

E

T

V

Q

K

I

V

A

Q

V

A

A

A

R

R

Q

H

M

L

V

V

H

Y

H

Q

A

A

A

A

A

I

L

N

R

K

E

D

A

L

G

G

R

K

P

P

A

Y

L

G

V

V

E

E

A

A

S

A

M

M

A

A

K

K

L

L

F

F

A

A

S

A

E

E

M

T

A

A

E

M

R

E

V

V

C

T

S

T

A

K

A

A

I

V

Q
|
Q

T
x
L

L

H

G
|
G

G
|
G

Y

Y

G

G

Y

Y

L

T

S

R

D

D

F

Y

P

P

V

V

E

E

R

R

I

M

Y

M

R

R

D

D

V

A

R

K

V

I

C

T

Q

E

I

I

Y
|
Y

E
|
E

G
|
G

T
|
T

S

S

D
x
E

I

V

Q

Q

R

R

L

M

V

V

I

I

S

S

R
x
G

N

K

L

L

active site: L124 (= L123), T125 (= T124), G241 (= G240), G374 (≠ R373)
binding calcium ion: E29 (≠ Q28), E33 (= E32), R35 (= R34)
binding coenzyme a persulfide: L238 (= L237), R242 (= R241), E362 (= E361), G363 (= G362)
binding flavin-adenine dinucleotide: F122 (= F121), L124 (= L123), T125 (= T124), P127 (= P126), T131 (≠ S130), F155 (= F154), I156 (= I155), T157 (= T156), E198 (= E197), R267 (= R266), F270 (= F269), L274 (≠ I273), F277 (≠ H276), Q335 (= Q334), L336 (≠ T335), G338 (= G337), G339 (= G338), Y361 (= Y360), T364 (= T363), E366 (≠ D365)

1jqiA Crystal structure of rat short chain acyl-coa dehydrogenase complexed with acetoacetyl-coa (see paper)
47% identity, 99% coverage: 2:375/379 of query aligns to 7:379/384 of 1jqiA

query
sites
1jqiA

L

L

P

P

N

E

E

T

D

H

Q

Q

C

M

A

-

I

L

A

R

E

Q

M

T

A

C

R

R

Q

D

F

F

A

A

R

E

E

K

R

E

L

L

K

V

P

P

F

I

A

A

E

A

Q

Q

W

L

S

D

R

K

E

E

H

H

R

L

Y

F

P

P

A

T

E

S

A

Q

I

V

G

K

E

K

M

M

A

G

A

E

L

L

G

G

F

L

F

L

G

A

M

M

L

D

V

V

P

P

E

E

Q

E

W

L

G

S

G

G

S

A

D

G

T

L

G

D

Y

Y

L

L

A

A

Y

Y

A

S

M

I

A

A

L

L

E

E

E

E

I

I

A

S

A

R

G

G

D

C

G

A

A

S

C

T

S

G

T

V

I

I

M

M

S

S

V

V

H

N

N

N

S

S

V
x
L

G

Y

C

L

V

G

P

P

I

I

L

L

R

K

F

F

G

G

N

S

D

S

Q

Q

Q

Q

K

K

R

Q

D

Q

F

W

L

I

G

T

P

P

L

F

A

T

R

N

G

G

E

D

Q

K

I

I

G

G

A

C

F
|
F

A

A

L
|
L

T
x
S

E

E

P

P

Q

G

A

N

G
|
G

S
|
S

D

D

A

A

S

G

S

A

L

A

R

S

T

T

R

T

A

A

R

R

R

E

D

E

G

G

D

D

H

S

Y

W

V

V

L

L

N

N

G

G

A

T

K

K

Q

A

F
x
W

I

I

T
|
T

S

N

G

S

K

W

H

E

A

A

G

S

T

A

V

T

I

V

V

V

F

F

A

A

V

S

T

T

D

D

P

R

D

S

A

R

G

Q

K

N

R

K

G

G

I

I

S

S

A

A

F

F

I

L

V

V

P

P

T

M

D

P

S

T

P

P

G

G

Y

L

Q

T

V

L

V

G

R

K

V

K

E

E

D

D

K

K

L

L

G

G

Q

I

H

R

A

A

S

S

D

S

T

T

C

A

Q

N

I

L

A

I

F

F

E

E

D

D

L

C

R

R

V

I

P

P

V

K

A

E

N

N

R

L

L

L

G

G

E

E

E

P

G

G

E

M

G

G

Y
x
F

R

K

I

I

A

A

L
x
M

A
x
Q

N

T

L
|
L

E
x
D

G

M

G

G

R
|
R

I

I

G

G

I

I

A

A

A

S

Q

Q

A

A

V

L

G

G

M

I

A

A

R

Q

A

A

A

S

F

L

E

D

A

C

A

A

R

V

D

K

Y

Y

A

A

G

E

E

N

R
|
R

E

H

T

A

F
|
F

G

G

K

A

P

P

I

L

I

T

E

K

H
x
L

Q

Q

A

N

V

I

A

Q

F

F

R

K

L

L

A

A

D

D

M

M

A

A

T

L

Q

A

I

L

A

E

V

S

A

A

R

R

Q

L

M

L

V

T

H

W

H

R

A

A

A

A

A

M

L

L

R

K

E

D

A

N

G

K

R

K

P

P

A

F

L

T

V

K

E

E

A

S

S

A

M

M

A

A

K

K

L

L

F

A

A

A

S

S

E

E

M

A

A

A

E

T

R

A

V

I

C

S

S

H

A

Q

A

A

I

I

Q
|
Q

T
x
I

L

L

G

G

G
|
G

Y

M

G

G

Y

Y

L

V

S

T

D

E

F

M

P

P

V

A

E

E

R

R

I

Y

Y

Y

R

R

D

D

V

A

R

R

V
x
I

C

T

Q

E

I

I

Y
|
Y

E
|
E

G
|
G

T
|
T

S

S

D
x
E

I
|
I

Q

Q

R

R

L

L

V

V

I

I

S

A

R
x
G

N

H

L

L

active site: G377 (≠ R373)
binding acetoacetyl-coenzyme a: L95 (≠ V91), F125 (= F121), S134 (= S130), F234 (≠ Y230), M238 (≠ L234), Q239 (≠ A235), L241 (= L237), D242 (≠ E238), R245 (= R241), Y364 (= Y360), E365 (= E361), G366 (= G362)
binding flavin-adenine dinucleotide: F125 (= F121), L127 (= L123), S128 (≠ T124), G133 (= G129), S134 (= S130), W158 (≠ F154), T160 (= T156), R270 (= R266), F273 (= F269), L280 (≠ H276), Q338 (= Q334), I339 (≠ T335), G342 (= G338), I360 (≠ V356), T367 (= T363), E369 (≠ D365), I370 (= I366)

P15651 Short-chain specific acyl-CoA dehydrogenase, mitochondrial; SCAD; Butyryl-CoA dehydrogenase; EC 1.3.8.1 from Rattus norvegicus (Rat) (see 2 papers)
47% identity, 99% coverage: 2:375/379 of query aligns to 34:406/412 of P15651

query
sites
P15651

L

L

P

P

N

E

E

T

D

H

Q

Q

C

M

A

-

I

L

A

R

E

Q

M

T

A

C

R

R

Q

D

F

F

A

A

R

E

E

K

R

E

L

L

K

V

P

P

F

I

A

A

E

A

Q

Q

W

L

S

D

R

K

E

E

H

H

R

L

Y

F

P

P

A

T

E

S

A

Q

I

V

G

K

E

K

M

M

A

G

A

E

L

L

G

G

F

L

F

L

G

A

M

M

L

D

V

V

P

P

E

E

Q

E

W

L

G

S

G

G

S

A

D

G

T

L

G

D

Y

Y

L

L

A

A

Y

Y

A

S

M

I

A

A

L

L

E

E

E

E

I

I

A

S

A

R

G

G

D

C

G

A

A

S

C

T

S

G

T

V

I

I

M

M

S

S

V

V

H

N

N

N

S

S

V

L

G

Y

C

L

V

G

P

P

I

I

L

L

R

K

F

F

G

G

N

S

D

S

Q

Q

Q

Q

K

K

R

Q

D

Q

F

W

L

I

G

T

P

P

L

F

A

T

R

N

G

G

E

D

Q

K

I

I

G

G

A

C

F
|
F

A
|
A

L
|
L

T
x
S

E
|
E

P
|
P

Q
x
G

A
x
N

G
|
G

S
|
S

D

D

A

A

S

G

S

A

L

A

R

S

T

T

R

T

A

A

R

R

R

E

D

E

G

G

D

D

H

S

Y

W

V

V

L

L

N

N

G

G

A

T

K

K

Q

A

F
x
W

I
|
I

T
|
T

S

N

G

S

K

W

H

E

A

A

G

S

T

A

V

T

I

V

V

V

F

F

A

A

V

S

T

T

D

D

P

R

D

S

A

R

G

Q

K

N

R

K

G

G

I

I

S

S

A

A

F

F

I

L

V

V

P

P

T

M

D

P

S

T

P

P

G

G

Y

L

Q

T

V

L

V

G

R

K

V

K

E

E

D

D

K

K

L

L

G

G

Q

I

H

R

A

A

S

S

D

S

T

T

C

A

Q

N

I

L

A

I

F

F

E

E

D

D

L

C

R

R

V

I

P

P

V

K

A

E

N

N

R

L

L

L

G

G

E

E

E

P

G

G

E

M

G

G

Y

F

R

K

I

I

A

A

L

M

A

Q

N

T

L

L

E
x
D

G
x
M

G
|
G

R
|
R

I

I

G

G

I

I

A

A

A

S

Q

Q

A

A

V

L

G

G

M

I

A

A

R

Q

A

A

A

S

F

L

E

D

A

C

A

A

R

V

D

K

Y

Y

A

A

G

E

E

N

R
|
R

E

H

T

A

F

F

G

G

K

A

P

P

I

L

I

T

E

K

H

L

Q

Q

A

N

V

I

A

Q

F

F

R

K

L

L

A

A

D

D

M

M

A

A

T

L

Q

A

I

L

A

E

V

S

A

A

R

R

Q

L

M

L

V

T

H

W

H

R

A

A

A

A

A

M

L

L

R

K

E

D

A

N

G

K

R

K

P

P

A

F

L

T

V

K

E

E

A

S

S

A

M

M

A

A

K

K

L

L

F

A

A

A

S

S

E

E

M

A

A

A

E

T

R

A

V

I

C

S

S

H

A

Q

A

A

I

I

Q
|
Q

T
x
I

L
|
L

G
|
G

G
|
G

Y

M

G

G

Y

Y

L

V

S

T

D

E

F

M

P

P

V

A

E

E

R

R

I

Y

Y

Y

R

R

D

D

V

A

R

R

V

I

C

T

Q

E

I

I

Y

Y

E
|
E

G

G

T
|
T

S
|
S

D
x
E

I

I

Q

Q

R

R

L

L

V

V

I

I

S

A

R

G

N

H

L

L

152:161 (vs. 121:130, 70% identical) binding FAD
S161 (= S130) binding substrate
WIT 185:187 (≠ FIT 154:156) binding FAD
DMGR 269:272 (≠ EGGR 238:241) binding substrate
R297 (= R266) binding FAD
QILGG 365:369 (≠ QTLGG 334:338) binding FAD
E392 (= E361) active site, Proton acceptor
TSE 394:396 (≠ TSD 363:365) binding FAD

Sites not aligning to the query:

1:24 modified: transit peptide, Mitochondrion

8sgsA Human liver mitochondrial short-chain specific acyl-coa dehydrogenase (see paper)
47% identity, 99% coverage: 2:375/379 of query aligns to 4:376/381 of 8sgsA

query
sites
8sgsA

L

L

P

P

N

E

E

T

D

H

Q

Q

C

M

A

L

I

L

A

-

E

Q

M

T

A

C

R

R

Q

D

F

F

A

A

R

E

E

K

R

E

L

L

K

F

P

P

F

I

A

A

E

A

Q

Q

W

V

S

D

R

K

E

E

H

H

R

L

Y

F

P

P

A

A

E

A

A

Q

I

V

G

K

E

K

M

M

A

G

A

G

L

L

G

G

F

L

F

L

G

A

M

M

L

D

V

V

P

P

E

E

Q

E

W

L

G

G

G

G

S

A

D

G

T

L

G

D

Y

Y

L

L

A

A

Y

Y

A

A

M

I

A

A

L

M

E

E

E

E

I

I

A

S

A

R

G

G

D

C

G

A

A

S

C

T

S

G

T

V

I

I

M

M

S

S

V

V

H

N

N

N

S

S

V

L

G

Y

C

L

V

G

P

P

I

I

L

L

R

K

F

F

G

G

N

S

D

K

Q

E

Q

Q

K

K

R

Q

D

A

F

W

L

V

G

T

P

P

L

F

A

T

R

S

G

G

E

D

Q

K

I

I

G

G

A

C

F
|
F

A

A

L
|
L

T
x
S

E

E

P

P

Q

G

A

N

G
|
G

S
|
S

D

D

A
|
A

S

G

S

A

L

A

R

S

T

T

R

T

A

A

R

R

R

A

D

E

G

G

D

D

H

S

Y

W

V

V

L

L

N

N

G

G

A

T

K

K

Q

A

F
x
W

I

I

T
|
T

S

N

G

A

K

W

H

E

A

A

G

S

T

A

V

A

I

V

V

V

F

F

A

A

V

S

T

T

D

D

P

R

D

A

A

L

G

Q

K
x
N

R

K

G

G

I

I

S

S

A

A

F

F

I

L

V

V

P

P

T

M

D

P

S

T

P

P

G

G

Y

L

Q

T

V

L

V

G

R

K

V

K

E

E

D

D

K

K

L

L

G

G

Q

I

H

R

A

G

S

S

D

S

T

T

C

A

Q

N

I

L

A

I

F

F

E

E

D

D

L

C

R

R

V

I

P

P

V

K

A

D

N

S

R

I

L

L

G

G

E

E

E

P

G

G

E

M

G

G

Y
x
F

R

K

I

I

A

A

L
x
M

A

Q

N

T

L
|
L

E

D

G

M

G

G

R

R

I

I

G

G

I

I

A

A

A

S

Q

Q

A

A

V

L

G

G

M

I

A

A

R

Q

A

T

A

A

F

L

E

D

A

C

A

A

R

V

D

N

Y

Y

A

A

G

E

E

N

R
|
R

E

M

T

A

F
|
F

G

G

K

A

P

P

I
x
L

I

T

E

K

H
x
L

Q

Q

A

V

V

I

A

Q

F

F

R

K

L

L

A

A

D

D

M

M

A

A

T

L

Q

A

I

L

A

E

V

S

A

A

R

R

Q

L

M

L

V

T

H

W

H

R

A

A

A

A

A

M

L

L

R

K

E

D

A

N

G

K

R

K

P

P

A

F

L
x
I

V

K

E

E

A

A

S

A

M

M

A

A

K

K

L

L

F

A

A

A

S

S

E

E

M

A

A

A

E

T

R

A

V

I

C

S

S

H

A

Q

A

A

I

I

Q
|
Q

T
x
I

L

L

G
|
G

G
|
G

Y

M

G

G

Y

Y

L

V

S

T

D

E

F

M

P

P

V

A

E

E

R

R

I

H

Y

Y

R

R

D

D

V

A

R

R

V
x
I

C

T

Q

E

I
|
I

Y
|
Y

E
|
E

G
|
G

T
|
T

S

S

D
x
E

I

I

Q

Q

R

R

L

L

V

V

I

I

S

A

R

G

N

H

L

L

binding coenzyme a: S131 (= S130), A133 (= A132), N177 (≠ K176), F231 (≠ Y230), M235 (≠ L234), L238 (= L237), I312 (≠ L311), E362 (= E361), G363 (= G362)
binding flavin-adenine dinucleotide: F122 (= F121), L124 (= L123), S125 (≠ T124), G130 (= G129), S131 (= S130), W155 (≠ F154), T157 (= T156), R267 (= R266), F270 (= F269), L274 (≠ I273), L277 (≠ H276), Q335 (= Q334), I336 (≠ T335), G338 (= G337), G339 (= G338), I357 (≠ V356), I360 (= I359), Y361 (= Y360), T364 (= T363), E366 (≠ D365)

7y0bA Crystal structure of human short-chain acyl-coa dehydrogenase
47% identity, 99% coverage: 2:375/379 of query aligns to 7:379/385 of 7y0bA

query
sites
7y0bA

L

L

P

P

N

E

E

T

D

H

Q

Q

C

M

A

L

I

L

A

-

E

Q

M

T

A

C

R

R

Q

D

F

F

A

A

R

E

E

K

R

E

L

L

K

F

P

P

F

I

A

A

E

A

Q

Q

W

V

S

D

R

K

E

E

H

H

R

L

Y

F

P

P

A

A

E

A

A

Q

I

V

G

K

E

K

M

M

A

G

A

G

L

L

G

G

F

L

F

L

G

A

M

M

L

D

V

V

P

P

E

E

Q

E

W

L

G

G

G

G

S

A

D

G

T

L

G

D

Y

Y

L

L

A

A

Y

Y

A

A

M

I

A

A

L

M

E

E

E

E

I

I

A

S

A

R

G

G

D

C

G

A

A

S

C

T

S

G

T

V

I

I

M

M

S

S

V

V

H

N

N

N

S

S

V

L

G

Y

C

L

V

G

P

P

I

I

L

L

R

K

F

F

G

G

N

S

D

K

Q

E

Q

Q

K

K

R

Q

D

A

F

W

L

V

G

T

P

P

L

F

A

T

R

S

G

G

E

D

Q

K

I

I

G

G

A

C

F
|
F

A

A

L
|
L

T
x
S

E

E

P

P

Q

G

A

N

G
|
G

S
|
S

D

D

A

A

S

G

S

A

L

A

R

S

T

T

R

T

A

A

R

R

R

A

D

E

G

G

D

D

H

S

Y

W

V

V

L

L

N

N

G

G

A

T

K

K

Q

A

F
x
W

I

I

T
|
T

S

N

G

A

K

W

H

E

A

A

G

S

T

A

V

A

I

V

V

V

F

F

A

A

V

S

T

T

D

D

P

R

D

A

A

L

G

Q

K

N

R

K

G

G

I

I

S

S

A

A

F

F

I

L

V

V

P

P

T

M

D

P

S

T

P

P

G

G

Y

L

Q

T

V

L

V

G

R

K

V

K

E

E

D

D

K

K

L

L

G

G

Q

I

H

R

A

G

S

S

D

S

T

T

C

A

Q

N

I

L

A

I

F

F

E

E

D

D

L

C

R

R

V

I

P

P

V

K

A

D

N

S

R

I

L

L

G

G

E

E

E

P

G

G

E

M

G

G

Y

F

R

K

I

I

A

A

L

M

A

Q

N

T

L

L

E

D

G

M

G

G

R

R

I

I

G

G

I

I

A

A

A

S

Q

Q

A

A

V

L

G

G

M

I

A

A

R

Q

A

T

A

A

F

L

E

D

A

C

A

A

R

V

D

N

Y

Y

A

A

G

E

E

N

R
|
R

E

M

T

A

F
|
F

G

G

K

A

P

P

I

L

I

T

E

K

H
x
L

Q

Q

A
x
V

V

I

A

Q

F

F

R

K

L

L

A

A

D

D

M

M

A

A

T

L

Q

A

I

L

A

E

V

S

A

A

R

R

Q

L

M

L

V

T

H

W

H

R

A

A

A

A

A

M

L

L

R

K

E

D

A

N

G

K

R

K

P

P

A

F

L

I

V

K

E

E

A

A

S

A

M

M

A

A

K

K

L

L

F

A

A

A

S

S

E

E

M

A

A

A

E

T

R

A

V

I

C

S

S

H

A

Q

A

A

I

I

Q
|
Q

T
x
I

L

L

G

G

G
|
G

Y
x
M

G

G

Y

Y

L

V

S
x
T

D
x
E

F

M

P

P

V

A

E

E

R

R

I

H

Y

Y

R

R

D

D

V

A

R

R

V
x
I

C

T

Q

E

I

I

Y
|
Y

E

E

G

G

T
|
T

S

S

D
x
E

I
|
I

Q

Q

R

R

L
|
L

V

V

I

I

S

A

R

G

N

H

L

L

binding (2S,3R,4R,5S,6R)-2-[4-chloranyl-3-[[4-[(3S)-oxolan-3-yl]oxyphenyl]methyl]phenyl]-6-(hydroxymethyl)oxane-3,4,5-triol: M343 (≠ Y339), T347 (≠ S343), E348 (≠ D344)
binding flavin-adenine dinucleotide: F125 (= F121), L127 (= L123), S128 (≠ T124), G133 (= G129), S134 (= S130), W158 (≠ F154), T160 (= T156), R270 (= R266), F273 (= F269), L280 (≠ H276), V282 (≠ A278), Q338 (= Q334), I339 (≠ T335), G342 (= G338), I360 (≠ V356), Y364 (= Y360), T367 (= T363), E369 (≠ D365), I370 (= I366), L373 (= L369)

7y0aC Crystal structure of human short-chain acyl-coa dehydrogenase
47% identity, 99% coverage: 2:375/379 of query aligns to 10:382/387 of 7y0aC

query
sites
7y0aC

L

L

P

P

N

E

E

T

D

H

Q

Q

C

M

A

L

I

L

A

-

E

Q

M

T

A

C

R

R

Q

D

F

F

A

A

R

E

E

K

R

E

L

L

K

F

P

P

F

I

A

A

E

A

Q

Q

W

V

S

D

R

K

E

E

H

H

R

L

Y

F

P

P

A

A

E

A

A

Q

I

V

G

K

E

K

M

M

A

G

A

G

L

L

G

G

F

L

F

L

G

A

M

M

L

D

V

V

P

P

E

E

Q

E

W

L

G

G

G

G

S

A

D

G

T

L

G

D

Y

Y

L

L

A

A

Y

Y

A

A

M

I

A

A

L

M

E

E

E

E

I

I

A

S

A

R

G

G

D

C

G

A

A

S

C

T

S

G

T

V

I

I

M

M

S

S

V

V

H

N

N

N

S

S

V

L

G

Y

C

L

V

G

P

P

I

I

L

L

R

K

F

F

G

G

N

S

D

K

Q

E

Q

Q

K

K

R

Q

D

A

F

W

L

V

G

T

P

P

L

F

A

T

R

S

G

G

E

D

Q

K

I

I

G

G

A

C

F
|
F

A

A

L
|
L

T
x
S

E

E

P

P

Q

G

A

N

G
|
G

S
|
S

D

D

A

A

S

G

S

A

L

A

R

S

T

T

R

T

A

A

R

R

R

A

D

E

G

G

D

D

H

S

Y

W

V

V

L

L

N

N

G

G

A

T

K

K

Q

A

F
x
W

I

I

T
|
T

S

N

G

A

K

W

H

E

A

A

G

S

T

A

V

A

I

V

V

V

F

F

A

A

V

S

T

T

D

D

P

R

D

A

A

L

G

Q

K

N

R

K

G

G

I

I

S

S

A

A

F

F

I

L

V

V

P

P

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binding flavin-adenine dinucleotide: F128 (= F121), L130 (= L123), S131 (≠ T124), G136 (= G129), S137 (= S130), W161 (≠ F154), T163 (= T156), T214 (= T207), R273 (= R266), F276 (= F269), L280 (≠ I273), L283 (≠ H276), V285 (≠ A278), Q341 (= Q334), I342 (≠ T335), G345 (= G338), I363 (≠ V356), Y367 (= Y360), T370 (= T363), E372 (≠ D365), L376 (= L369)

P16219 Short-chain specific acyl-CoA dehydrogenase, mitochondrial; SCAD; Butyryl-CoA dehydrogenase; EC 1.3.8.1 from Homo sapiens (Human) (see 3 papers)
47% identity, 99% coverage: 2:375/379 of query aligns to 34:406/412 of P16219

query
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P16219

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G90 (= G59) to S: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs121908005
E104 (= E73) natural variant: Missing (in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs387906308)
152:161 (vs. 121:130, 70% identical) binding in other chain
R171 (= R140) to W: 69% of wild-type acyl-CoA dehydrogenase activity; confers susceptibility to ethylmalonicaciduria; dbSNP:rs1800556
WIT 185:187 (≠ FIT 154:156) binding in other chain
A192 (= A161) to V: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs28940874
G209 (= G178) to S: 86% of wild-type acyl-CoA dehydrogenase activity; confers susceptibility to ethylmalonicaciduria; dbSNP:rs1799958
R297 (= R266) binding FAD
Q308 (= Q277) binding in other chain
R325 (= R294) to W: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs121908006
S353 (= S322) to L: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs28941773
QILGG 365:369 (≠ QTLGG 334:338) binding FAD
R380 (= R349) to W: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs28940875
TSE 394:396 (≠ TSD 363:365) binding in other chain

Sites not aligning to the query:

1:24 modified: transit peptide, Mitochondrion

2vigB Crystal structure of human short-chain acyl coa dehydrogenase
46% identity, 99% coverage: 2:375/379 of query aligns to 1:366/371 of 2vigB

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