SitesBLAST
Comparing WP_011913318.1 NCBI__GCF_000013785.1:WP_011913318.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 19 hits to proteins with known functional sites (download)
4wjmA Crystal structure of fructokinase from brucella abortus 2308 with bound amppnp
35% identity, 100% coverage: 1:309/310 of query aligns to 7:312/312 of 4wjmA
- active site: G252 (= G249), A253 (= A250), G254 (= G251), D255 (= D252)
- binding phosphoaminophosphonic acid-adenylate ester: T223 (= T219), G225 (= G221), A226 (≠ R222), G228 (= G224), G252 (= G249), A253 (= A250), G254 (= G251), V257 (≠ F254), V292 (≠ S289), A295 (= A292)
7fcaD Pfkb(mycobacterium marinum) (see paper)
34% identity, 96% coverage: 3:300/310 of query aligns to 5:281/282 of 7fcaD
3gbuA Crystal structure of an uncharacterized sugar kinase ph1459 from pyrococcus horikoshii in complex with atp
28% identity, 95% coverage: 5:300/310 of query aligns to 5:282/302 of 3gbuA
- active site: G242 (= G249), A243 (= A250), G244 (= G251), D245 (= D252)
- binding adenosine-5'-triphosphate: K188 (= K186), T213 (= T219), G215 (= G221), V235 (≠ A242), P237 (≠ V244), A243 (= A250), G244 (= G251), A274 (= A292)
3ih0A Crystal structure of an uncharacterized sugar kinase ph1459 from pyrococcus horikoshii in complex with amp-pnp
28% identity, 95% coverage: 5:300/310 of query aligns to 6:283/304 of 3ih0A
5eynA Crystal structure of fructokinase from vibrio cholerae o395 in fructose, adp, beryllium trifluoride and calcium ion bound form
30% identity, 75% coverage: 30:263/310 of query aligns to 27:260/306 of 5eynA
- active site: G246 (= G249), A247 (= A250), G248 (= G251), D249 (= D252)
- binding adenosine-5'-diphosphate: H91 (≠ D93), T217 (= T219), G219 (= G221), A220 (≠ R222), A238 (≠ P241), V239 (≠ A242), T244 (≠ S247), G246 (= G249), A247 (= A250), G248 (= G251), F251 (= F254)
- binding beryllium trifluoride ion: G246 (= G249), G248 (= G251), D249 (= D252)
- binding beta-D-fructofuranose: G28 (= G31), A29 (≠ S32), N32 (= N35), F96 (≠ Y98), F98 (= F100), R159 (= R161), D249 (= D252)
Sites not aligning to the query:
5yggA Crystal structure of fructokinase double-mutant (t261c-h108c) from vibrio cholerae o395 in fructose, adp and potassium ion bound form (see paper)
30% identity, 75% coverage: 30:263/310 of query aligns to 31:264/310 of 5yggA
- binding adenosine-5'-diphosphate: K188 (= K186), T221 (= T219), G223 (= G221), A242 (≠ P241), V243 (≠ A242), F255 (= F254)
- binding beta-D-fructofuranose: G32 (= G31), A33 (≠ S32), F100 (≠ Y98), F102 (= F100), R163 (= R161), D253 (= D252)
Sites not aligning to the query:
3lkiB Crystal structure of fructokinase with bound atp from xylella fastidiosa
32% identity, 96% coverage: 3:300/310 of query aligns to 6:302/322 of 3lkiB
Q8ZKR2 Aminoimidazole riboside kinase; AIRs kinase; EC 2.7.1.223 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
29% identity, 85% coverage: 30:292/310 of query aligns to 30:293/319 of Q8ZKR2
- G31 (= G31) binding 5-amino-1-(beta-D-ribosyl)imidazole
- Y101 (≠ F100) binding 5-amino-1-(beta-D-ribosyl)imidazole
- R162 (= R161) binding 5-amino-1-(beta-D-ribosyl)imidazole
- A180 (= A179) binding K(+)
- A181 (≠ E180) binding K(+)
- A183 (= A182) binding K(+)
- G213 (≠ H212) binding K(+)
- D246 (= D246) binding K(+)
- T248 (≠ V248) binding K(+)
- D252 (= D252) binding 5-amino-1-(beta-D-ribosyl)imidazole
- A287 (≠ E286) binding K(+)
- A290 (≠ S289) binding K(+)
- G292 (≠ A291) binding K(+)
Sites not aligning to the query:
- 16 binding 5-amino-1-(beta-D-ribosyl)imidazole
1tz3A Crystal structure of aminoimidazole riboside kinase complexed with aminoimidazole riboside (see paper)
29% identity, 85% coverage: 30:292/310 of query aligns to 26:282/299 of 1tz3A
- active site: F88 (≠ Y98), G238 (= G249), A239 (= A250), G240 (= G251), D241 (= D252)
- binding 5-aminoimidazole ribonucleoside: G27 (= G31), L83 (≠ V88), F88 (≠ Y98), Y90 (≠ F100), R151 (= R161), M154 (≠ V164), D241 (= D252)
Sites not aligning to the query:
1tz6A Crystal structure of aminoimidazole riboside kinase from salmonella enterica complexed with aminoimidazole riboside and atp analog (see paper)
29% identity, 85% coverage: 30:292/310 of query aligns to 26:282/297 of 1tz6A
- active site: F88 (≠ Y98), G238 (= G249), A239 (= A250), G240 (= G251), D241 (= D252)
- binding phosphomethylphosphonic acid adenylate ester: N149 (= N159), K176 (= K186), E181 (≠ D191), S209 (≠ T219), G211 (= G221), A212 (≠ R222), G214 (= G224), A239 (= A250), G240 (= G251), F243 (= F254), N270 (≠ Q280), G273 (≠ A283), A274 (≠ M284)
- binding 5-aminoimidazole ribonucleoside: G27 (= G31), F88 (≠ Y98), Y90 (≠ F100), R151 (= R161), M154 (≠ V164), D241 (= D252)
Sites not aligning to the query:
1v1aA 2-keto-3-deoxygluconate kinase from thermus thermophilus with bound 2- keto-3-deoxygluconate and adp (see paper)
33% identity, 78% coverage: 20:260/310 of query aligns to 24:259/301 of 1v1aA
- active site: G248 (= G249), A249 (= A250), G250 (= G251), D251 (= D252)
- binding adenosine-5'-diphosphate: K219 (≠ T219), G221 (= G221), A222 (≠ R222), A249 (= A250), G250 (= G251)
- binding 2-keto-3-deoxygluconate: G34 (= G31), A35 (≠ S32), N38 (= N35), Y89 (≠ A86), R105 (= R101), R167 (= R161), G248 (= G249), D251 (= D252)
Sites not aligning to the query:
Q53W83 2-dehydro-3-deoxygluconokinase; 2-keto-3-deoxygluconokinase; 3-deoxy-2-oxo-D-gluconate kinase; KDG kinase; EC 2.7.1.45 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
33% identity, 78% coverage: 20:260/310 of query aligns to 24:259/309 of Q53W83
- GAEVN 34:38 (≠ GSPYN 31:35) binding substrate
- YYR 103:105 (≠ SFR 99:101) binding substrate
- R167 (= R161) binding substrate
- S193 (= S188) binding ATP
- 219:225 (vs. 219:225, 57% identical) binding ATP
- GAGD 248:251 (= GAGD 249:252) binding ATP
- D251 (= D252) binding substrate
Sites not aligning to the query:
- 275 binding ATP
- 287 binding substrate
1v1bA 2-keto-3-deoxygluconate kinase from thermus thermophilus with bound atp (see paper)
33% identity, 78% coverage: 20:260/310 of query aligns to 24:259/300 of 1v1bA
- active site: G248 (= G249), A249 (= A250), G250 (= G251), D251 (= D252)
- binding adenosine-5'-triphosphate: K219 (≠ T219), G221 (= G221), A238 (= A239), F239 (≠ Q240), V241 (≠ A242), G248 (= G249), A249 (= A250), G250 (= G251)
Sites not aligning to the query:
3iq0B Crystal structure of a putative ribokinase ii in complex with atp and mg+2 from e.Coli
24% identity, 87% coverage: 31:301/310 of query aligns to 36:291/308 of 3iq0B
- active site: G252 (= G249), A253 (= A250), G254 (= G251), D255 (= D252)
- binding adenosine-5'-triphosphate: S192 (= S188), K223 (≠ T219), G225 (= G221), E247 (≠ V244), A253 (= A250), G254 (= G251), F257 (= F254), N279 (≠ S289), G282 (≠ A292)
Q9M394 Fructokinase-like 1, chloroplastic; PEP-associated protein 6; pfkB-type carbohydrate kinase family protein 2 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
23% identity, 75% coverage: 30:263/310 of query aligns to 148:416/471 of Q9M394
Sites not aligning to the query:
- 105 C→A: Does not affect the interaction with CITRX.
- 105:106 CC→AA: Abolishes interaction with CITRX.
- 106 C→A: Strongly reduces the interaction with CITRX.
4xckA Vibrio cholerae o395 ribokinase complexed with adp, ribose and cesium ion. (see paper)
26% identity, 96% coverage: 13:309/310 of query aligns to 21:298/306 of 4xckA
- active site: A249 (≠ G249), A250 (= A250), G251 (= G251), D252 (= D252)
- binding adenosine-5'-diphosphate: T220 (= T219), G222 (= G221), S223 (≠ R222), V242 (≠ A242), T247 (≠ S247), A250 (= A250), F254 (= F254), H276 (≠ S289), A279 (= A292), V283 (≠ C296)
- binding alpha-D-ribofuranose: G39 (= G31), K40 (≠ S32), N43 (= N35), A95 (= A86), I107 (≠ Y98), I109 (≠ F100), E140 (= E139), T248 (≠ V248), D252 (= D252)
Sites not aligning to the query:
3in1A Crystal structure of a putative ribokinase in complex with adp from e.Coli
24% identity, 93% coverage: 9:295/310 of query aligns to 23:288/312 of 3in1A
- active site: R106 (≠ P96), G255 (= G249), A256 (= A250), G257 (= G251), D258 (= D252)
- binding adenosine-5'-diphosphate: N194 (≠ V187), K225 (≠ T219), G227 (= G221), G230 (= G224), A244 (= A238), T253 (≠ S247), N282 (≠ S289), A285 (= A292)
F4I0K2 Fructokinase-like 2, chloroplastic; pfkB-type carbohydrate kinase family protein 1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
23% identity, 76% coverage: 30:265/310 of query aligns to 251:509/614 of F4I0K2
Sites not aligning to the query:
- 208 C→A: Strongly reduces the interaction with CITRX.
- 208:209 CC→AA: Abolishes interaction with CITRX.
- 209 C→A: Strongly reduces the interaction with CITRX.
2varA Crystal structure of sulfolobus solfataricus 2-keto-3-deoxygluconate kinase complexed with 2-keto-3-deoxygluconate (see paper)
20% identity, 76% coverage: 25:260/310 of query aligns to 27:265/311 of 2varA
- active site: G254 (= G249), A255 (= A250), G256 (= G251), D257 (= D252)
- binding adenosine monophosphate: G227 (= G221), G230 (= G224), M259 (≠ F254)
- binding phosphoaminophosphonic acid-adenylate ester: K225 (≠ T219), G227 (= G221), S228 (≠ R222), G230 (= G224), G254 (= G249), A255 (= A250), G256 (= G251), D257 (= D252), M259 (≠ F254)
- binding 3-deoxy-alpha-D-erythro-hex-2-ulofuranosonic acid: G33 (= G31), S34 (= S32), Y89 (≠ M87), Y105 (≠ F100), R107 (≠ G102), I136 (≠ Y130), R165 (= R161), G254 (= G249), D257 (= D252)
- binding 2-keto-3-deoxygluconate: G33 (= G31), S34 (= S32), Y89 (≠ M87), L103 (vs. gap), Y105 (≠ F100), R107 (≠ G102), I136 (≠ Y130), R165 (= R161), T253 (≠ V248), G254 (= G249), D257 (= D252)
Sites not aligning to the query:
Query Sequence
>WP_011913318.1 NCBI__GCF_000013785.1:WP_011913318.1
MYLVCGEALFDVFTGPGASGNRLNLEAVAGGSPYNVAVGLARLDVKAALLGGLSNDHFGQ
RLLGLLQKEGVDTGQLVIFDAPTTLAMVALGSDGAPVYSFRGDGCADRLLQPEQLRPLPD
RVRGIHFGSYSLVASPIAETLLELLRREQAQRLISLDPNVRLNVEPDLARWRERVEAFAE
QAHLIKVSDEDLRLLYPGVEPHGIAERWLGNHAELVVITRGRDGAQLFSRQHGQLAIAAQ
PATVCDSVGAGDTFQAALLAYLTEQRLDSPAGLAQMSPAQLQAMLEFASKAAAITCSRRG
PELPYRYEIA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory