SitesBLAST

P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
32% identity, 93% coverage: 5:557/593 of query aligns to 95:638/667 of P09342

query
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C161 (= C72) modified: Disulfide link with 307
P194 (= P105) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
C307 (≠ I214) modified: Disulfide link with 161

P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
32% identity, 93% coverage: 5:557/593 of query aligns to 92:635/664 of P09114

query
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P09114

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P191 (= P105) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
W568 (≠ A482) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
32% identity, 93% coverage: 5:556/593 of query aligns to 13:555/582 of 8et4A

query
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8et4A

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binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (≠ I393), G401 (= G394), Q402 (≠ L395), H403 (≠ S396), G426 (= G419), M428 (≠ L421), G452 (= G445), D453 (= D446), G454 (≠ Y447), S455 (≠ D448), M458 (≠ F451), N480 (= N473), H482 (≠ Y475), L483 (= L476), G484 (= G477), M485 (≠ L478), V486 (≠ I479)
binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ C255), M266 (≠ L257), H267 (≠ Q258), G286 (= G278), V287 (≠ N279), R288 (= R280), D290 (≠ A282), R292 (= R284), V293 (≠ H285), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ A322), M405 (≠ I398), G423 (= G416)
binding magnesium ion: F370 (≠ R361), D453 (= D446), M458 (≠ F451), Q461 (≠ E454), N480 (= N473), H482 (≠ Y475), K533 (≠ Q530)
binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (≠ L257), R292 (= R284), M485 (≠ L478), W489 (≠ A482)

Sites not aligning to the query:

binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: 568

5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
32% identity, 93% coverage: 5:556/593 of query aligns to 13:555/582 of 5wj1A

query
sites
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active site: Y33 (≠ I25), G35 (= G27), G36 (≠ A28), A37 (= A29), S38 (≠ I30), E59 (≠ V51), T82 (= T75), F121 (= F114), Q122 (= Q115), E123 (≠ A116), K171 (≠ F164), M266 (≠ L257), V293 (≠ H285), V400 (≠ I393), G426 (= G419), M428 (≠ L421), D453 (= D446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (≠ L478), V486 (≠ I479), W489 (≠ A482)
binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), M263 (= M254), L264 (≠ C255), G286 (= G278), R288 (= R280), V293 (≠ H285), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ A322), M405 (≠ I398), G423 (= G416), G424 (≠ Q417)
binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ Y475)
binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (≠ L257), D291 (≠ N283), R292 (= R284), M485 (≠ L478), W489 (≠ A482)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (≠ I393), G401 (= G394), Q402 (≠ L395), H403 (≠ S396), M428 (≠ L421), D453 (= D446), G454 (≠ Y447), S455 (≠ D448), M458 (≠ F451), N480 (= N473), H482 (≠ Y475), L483 (= L476), G484 (= G477), M485 (≠ L478), V486 (≠ I479)

Sites not aligning to the query:

5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium (see paper)
32% identity, 93% coverage: 5:556/593 of query aligns to 13:555/582 of 5k6tA

query
sites
5k6tA

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x
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-

A

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F

V

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V

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F

T

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Q

K

E

A

D

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R

R

P

E

A

A

I

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E

Q

Q

T

A

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active site: Y33 (≠ I25), G35 (= G27), G36 (≠ A28), A37 (= A29), S38 (≠ I30), E59 (≠ V51), T82 (= T75), F121 (= F114), Q122 (= Q115), E123 (≠ A116), K171 (≠ F164), M266 (≠ L257), V293 (≠ H285), V400 (≠ I393), G426 (= G419), M428 (≠ L421), D453 (= D446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (≠ L478), V486 (≠ I479), W489 (≠ A482)
binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: H267 (≠ Q258), R292 (= R284), M485 (≠ L478), W489 (≠ A482)
binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ C255), G286 (= G278), R288 (= R280), D290 (≠ A282), R292 (= R284), V293 (≠ H285), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ A322), Q404 (= Q397), M405 (≠ I398), G423 (= G416)
binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ Y475)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (≠ I393), G401 (= G394), Q402 (≠ L395), H403 (≠ S396), G426 (= G419), M428 (≠ L421), G452 (= G445), G454 (≠ Y447), S455 (≠ D448), N480 (= N473), H482 (≠ Y475), L483 (= L476), G484 (= G477)

Sites not aligning to the query:

5k6rA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, thiencarbazone-methyl (see paper)
32% identity, 93% coverage: 5:556/593 of query aligns to 13:555/582 of 5k6rA

query
sites
5k6rA

R

K

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G

Y

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Y

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C

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A

Q

N

L

R

G

A

F

H

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T

N

F

I

L

N

-

A

G

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S

A

G

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P

A

A

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E

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D

L

L

R

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P

E

A

A

I

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E

Q

Q

T

A

-

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-

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-

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M

A

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active site: Y33 (≠ I25), G35 (= G27), G36 (≠ A28), A37 (= A29), S38 (≠ I30), E59 (≠ V51), T82 (= T75), F121 (= F114), Q122 (= Q115), E123 (≠ A116), K171 (≠ F164), M266 (≠ L257), V293 (≠ H285), V400 (≠ I393), G426 (= G419), M428 (≠ L421), D453 (= D446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (≠ L478), V486 (≠ I479), W489 (≠ A482)
binding methyl 4-[(3-methoxy-4-methyl-5-oxidanylidene-1,2,4-triazol-1-yl)carbonylsulfamoyl]-5-methyl-thiophene-3-carboxylate: R292 (= R284), W489 (≠ A482)
binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ C255), M266 (≠ L257), G286 (= G278), R288 (= R280), R292 (= R284), V293 (≠ H285), D310 (= D302), I311 (= I303), G328 (≠ S320), D329 (= D321), V330 (≠ A322), M405 (≠ I398), G423 (= G416)
binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ Y475)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (≠ I393), G401 (= G394), Q402 (≠ L395), H403 (≠ S396), G426 (= G419), M428 (≠ L421), D453 (= D446), G454 (≠ Y447), S455 (≠ D448), M458 (≠ F451), N480 (= N473), H482 (≠ Y475), L483 (= L476), G484 (= G477), M485 (≠ L478), V486 (≠ I479)

Sites not aligning to the query:

1z8nA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with an imidazolinone herbicide, imazaquin (see paper)
32% identity, 93% coverage: 5:556/593 of query aligns to 13:555/582 of 1z8nA

query
sites
1z8nA

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active site: Y33 (≠ I25), G35 (= G27), G36 (≠ A28), A37 (= A29), S38 (≠ I30), E59 (≠ V51), T82 (= T75), F121 (= F114), Q122 (= Q115), E123 (≠ A116), K171 (≠ F164), M266 (≠ L257), V293 (≠ H285), V400 (≠ I393), G426 (= G419), M428 (≠ L421), D453 (= D446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (≠ L478), V486 (≠ I479), W489 (≠ A482)
binding 2-(4-isopropyl-4-methyl-5-oxo-4,5-dihydro-1h-imidazol-2-yl)quinoline-3-carboxylic acid: K135 (= K128), R161 (= R154), Y191 (= Y184), R194 (vs. gap), D291 (≠ N283), R292 (= R284), D312 (≠ E304), W489 (≠ A482)
binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G211), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ C255), G265 (= G256), M266 (≠ L257), H267 (≠ Q258), G286 (= G278), V287 (≠ N279), R288 (= R280), D290 (≠ A282), R292 (= R284), V293 (≠ H285), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ A322), M405 (≠ I398), G423 (= G416), G424 (≠ Q417)
binding magnesium ion: D453 (= D446), N480 (= N473)
binding thiamine diphosphate: V400 (≠ I393), G401 (= G394), Q402 (≠ L395), H403 (≠ S396), G426 (= G419), M428 (≠ L421), G452 (= G445), G454 (≠ Y447), S455 (≠ D448), N480 (= N473), H482 (≠ Y475), L483 (= L476), G484 (= G477), M485 (≠ L478), V486 (≠ I479)

Sites not aligning to the query:

1yi1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
32% identity, 93% coverage: 5:556/593 of query aligns to 13:555/582 of 1yi1A

query
sites
1yi1A

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active site: Y33 (≠ I25), G35 (= G27), G36 (≠ A28), A37 (= A29), S38 (≠ I30), E59 (≠ V51), T82 (= T75), F121 (= F114), Q122 (= Q115), E123 (≠ A116), K171 (≠ F164), M266 (≠ L257), V293 (≠ H285), V400 (≠ I393), G426 (= G419), M428 (≠ L421), D453 (= D446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (≠ L478), V486 (≠ I479), W489 (≠ A482)
binding methyl 2-[4-methoxy-6-methyl-1,3,5-trazin-2-yl(methyl)carbamoylsulfamoyl]benzoate: D291 (≠ N283), R292 (= R284), W489 (≠ A482)
binding flavin-adenine dinucleotide: R161 (= R154), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), M263 (= M254), L264 (≠ C255), G265 (= G256), M266 (≠ L257), H267 (≠ Q258), G286 (= G278), V287 (≠ N279), R288 (= R280), D290 (≠ A282), V293 (≠ H285), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ A322), M405 (≠ I398), G423 (= G416), G424 (≠ Q417)
binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ Y475)

Sites not aligning to the query:

1yi0A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
32% identity, 93% coverage: 5:556/593 of query aligns to 13:555/582 of 1yi0A

query
sites
1yi0A

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active site: Y33 (≠ I25), G35 (= G27), G36 (≠ A28), A37 (= A29), S38 (≠ I30), E59 (≠ V51), T82 (= T75), F121 (= F114), Q122 (= Q115), E123 (≠ A116), K171 (≠ F164), M266 (≠ L257), V293 (≠ H285), V400 (≠ I393), G426 (= G419), M428 (≠ L421), D453 (= D446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (≠ L478), V486 (≠ I479), W489 (≠ A482)
binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (≠ N283), R292 (= R284), W489 (≠ A482)
binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ C255), G265 (= G256), M266 (≠ L257), H267 (≠ Q258), G286 (= G278), V287 (≠ N279), R288 (= R280), D290 (≠ A282), R292 (= R284), V293 (≠ H285), D310 (= D302), I311 (= I303), G328 (≠ S320), D329 (= D321), V330 (≠ A322), M405 (≠ I398), G423 (= G416), G424 (≠ Q417)
binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ Y475)

Sites not aligning to the query:

1yhzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
32% identity, 93% coverage: 5:556/593 of query aligns to 13:555/582 of 1yhzA

query
sites
1yhzA

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active site: Y33 (≠ I25), G35 (= G27), G36 (≠ A28), A37 (= A29), S38 (≠ I30), E59 (≠ V51), T82 (= T75), F121 (= F114), Q122 (= Q115), E123 (≠ A116), K171 (≠ F164), M266 (≠ L257), V293 (≠ H285), V400 (≠ I393), G426 (= G419), M428 (≠ L421), D453 (= D446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (≠ L478), V486 (≠ I479), W489 (≠ A482)
binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: D291 (≠ N283), R292 (= R284), M485 (≠ L478), W489 (≠ A482)
binding flavin-adenine dinucleotide: R161 (= R154), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ C255), M266 (≠ L257), H267 (≠ Q258), G286 (= G278), V287 (≠ N279), R288 (= R280), D290 (≠ A282), V293 (≠ H285), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ A322), Q404 (= Q397), M405 (≠ I398), G423 (= G416), G424 (≠ Q417)
binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ Y475)

Sites not aligning to the query:

1yhyA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
32% identity, 93% coverage: 5:556/593 of query aligns to 13:555/582 of 1yhyA

query
sites
1yhyA

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A

V

E

E

T

L

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T

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G

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P

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I

A

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T
|
T

L
|
L

M
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M

G

G

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L

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G

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Y

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P

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C

D

D

H

D

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E

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A

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x
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L
x
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x
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-

R

V

Y

Y

G

A

N

N

A

Y

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A

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E

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S

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I

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G

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x
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R

W

F

A
x
D

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x
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R

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x
V

T

T

G

G

S

K

V

L

E

E

T

A

Y

F

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A

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G

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A

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F

I

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H

H

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D

I
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I

E

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A

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E

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I

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G

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P

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Y

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I

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S

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D

A
x
V

R

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A

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A

A

L

L

E

Q

L

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F

M

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D

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V

A

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E

A

N

R

R

K

A

A

E

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E

G

L

R

K

L

L

P

-

D

D

R

F

R

G

A

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W

W

-

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-

N

A

E

A

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D

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Q

E

K

R

Q

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K

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M

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-

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-

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x
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Y

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W

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A

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E

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L

N

N

N
|
N

A

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x
H

L
|
L

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G

L
x
M

I
x
V

R

M

Q

Q

A
x
W

Q

E

R

D

G

R

F

F

D

-

M

-

D

-

Y

Y

C

K

V

A

Q

N

L

R

G

A

F

H

E

T

N

F

I

L

N

-

A

G

D

D

Q

P

S

A

G

Q

M

E

E

D

G

E

Y

I

G

F

V

P

D

N

H

M

V

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A

L

V

F

V

A

E

A

G

A

L

C

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C

I

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P

A

A

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A

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R

V

V

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T

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Q

K

E

A

D

D

L

L

R

R

P

E

A

A

I

I

E

Q

Q

T

A

-

Q

-

A

-

W

-

M

M

A

L

E

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I

active site: Y33 (≠ I25), G35 (= G27), G36 (≠ A28), A37 (= A29), S38 (≠ I30), E59 (≠ V51), T82 (= T75), F121 (= F114), Q122 (= Q115), E123 (≠ A116), K171 (≠ F164), M266 (≠ L257), V293 (≠ H285), V400 (≠ I393), G426 (= G419), M428 (≠ L421), D453 (= D446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (≠ L478), V486 (≠ I479), W489 (≠ A482)
binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (≠ N283), R292 (= R284), V486 (≠ I479), W489 (≠ A482)
binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ C255), G265 (= G256), M266 (≠ L257), H267 (≠ Q258), G286 (= G278), V287 (≠ N279), R288 (= R280), D290 (≠ A282), V293 (≠ H285), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ A322), Q404 (= Q397), M405 (≠ I398), G423 (= G416), G424 (≠ Q417)
binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ Y475)

Sites not aligning to the query:

1ybhA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide chlorimuron ethyl (see paper)
32% identity, 93% coverage: 5:556/593 of query aligns to 13:555/582 of 1ybhA

query
sites
1ybhA

R

K

A

G

I

A

D

D

A

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A

E

V

A

L

L

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E

K

R

E

Q

G

G

I

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F

G

A

I
x
Y

P

P

G
|
G

A
x
G

A
|
A

I
x
S

N

M

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E

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I

Y

H

S

Q

A

A

L

L

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T

A

R

D

S

G

S

I

I

R

R

H

N

I

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L

A

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R

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H

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x
E

E

Q

G

G

A

G

S

V

H

F

M

A

A

A

E

E

G

G

Y

Y

T

A

R

R

A

S

K

S

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-

G

G

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G

G

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I

C

C

I

I

G

A

T
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T

S

S

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G

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A

G

G

A

T

T

D

N

M

L

I

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G

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A

A

S

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A

L

D

D

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S

I

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I

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A

I

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T

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G

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Q

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A

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F

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E

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E

I

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G

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I

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L

L

V

L

N

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N
|
N

A

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x
H

L
|
L

G

G

L
x
M

I
x
V

R

M

Q

Q

A
x
W

Q

E

R

D

G

R

F

F

D

-

M

-

D

-

Y

Y

C

K

V

A

Q

N

L

R

G

A

F

H

E

T

N

F

I

L

N

-

A

G

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D

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S

A

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Q

M

E

E

D

G

E

Y

I

G

F

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D

N

H

M

V

L

A

L

V

F

V

A

E

A

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A

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C

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G

C

I

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P

A

A

L

A

R

R

V

V

F

T

K

K

Q

K

E

A

D

D

L

L

R

R

P

E

A

A

I

I

E

Q

Q

T

A

-

Q

-

A

-

W

-

M

M

A

L

E

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T

R

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L

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I

active site: Y33 (≠ I25), G35 (= G27), G36 (≠ A28), A37 (= A29), S38 (≠ I30), E59 (≠ V51), T82 (= T75), F121 (= F114), Q122 (= Q115), E123 (≠ A116), K171 (≠ F164), M266 (≠ L257), V293 (≠ H285), V400 (≠ I393), G426 (= G419), M428 (≠ L421), D453 (= D446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (≠ L478), V486 (≠ I479), W489 (≠ A482)
binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: M266 (≠ L257), D291 (≠ N283), R292 (= R284), M485 (≠ L478), W489 (≠ A482)
binding flavin-adenine dinucleotide: R161 (= R154), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ C255), M266 (≠ L257), H267 (≠ Q258), G286 (= G278), V287 (≠ N279), R288 (= R280), D290 (≠ A282), V293 (≠ H285), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ A322), Q404 (= Q397), M405 (≠ I398), G423 (= G416), G424 (≠ Q417)
binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ Y475)

Sites not aligning to the query:

5k3sA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, bispyribac-sodium (see paper)
32% identity, 93% coverage: 5:556/593 of query aligns to 13:555/583 of 5k3sA

query
sites
5k3sA

R

K

A

G

I

A

D

D

A

I

A

L

V

V

A

E

V

A

L

L

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E

K

R

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A

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F

F

G

A

I
x
Y

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|
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A
x
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A
|
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x
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A

A

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A

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D

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G

S

I

I

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x
E

E

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A

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E

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G

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Y

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G

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A

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x
E

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I

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E

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R

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A

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G

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active site: Y33 (≠ I25), G35 (= G27), G36 (≠ A28), A37 (= A29), S38 (≠ I30), E59 (≠ V51), T82 (= T75), F121 (= F114), Q122 (= Q115), E123 (≠ A116), K171 (≠ F164), M266 (≠ L257), V293 (≠ H285), V400 (≠ I393), G426 (= G419), M428 (≠ L421), D453 (= D446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (≠ L478), V486 (≠ I479), W489 (≠ A482)
binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: R292 (= R284), M485 (≠ L478), W489 (≠ A482)
binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ C255), M266 (≠ L257), G286 (= G278), R288 (= R280), D290 (≠ A282), V293 (≠ H285), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ A322), M405 (≠ I398), G423 (= G416)
binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ Y475)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (≠ I393), G401 (= G394), Q402 (≠ L395), H403 (≠ S396), G426 (= G419), M428 (≠ L421), D453 (= D446), G454 (≠ Y447), S455 (≠ D448), N480 (= N473), H482 (≠ Y475), L483 (= L476), G484 (= G477), M485 (≠ L478), V486 (≠ I479)

Sites not aligning to the query:

active site: 558
binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: 569

P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
32% identity, 93% coverage: 5:556/593 of query aligns to 98:640/670 of P17597

query
sites
P17597

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A122 (= A29) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
M124 (≠ N31) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
E144 (≠ V51) binding thiamine diphosphate
S186 (= S94) binding FAD
P197 (= P105) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
R199 (≠ A107) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
Q207 (= Q115) binding thiamine diphosphate
K220 (= K128) binding (R)-imazaquin
R246 (= R154) binding (R)-imazaquin; binding FAD
K256 (≠ F164) binding chlorimuron-ethyl
G308 (= G212) binding FAD
TL 331:332 (= TL 237:238) binding FAD
C340 (≠ D246) modified: Cysteine sulfinic acid (-SO2H)
LGMH 349:352 (≠ CGLQ 255:258) binding FAD
GVRFD 371:375 (≠ GNRWA 278:282) binding FAD
DR 376:377 (≠ NR 283:284) binding chlorimuron-ethyl
DI 395:396 (= DI 302:303) binding FAD
DV 414:415 (≠ DA 321:322) binding FAD
QH 487:488 (≠ LS 395:396) binding thiamine diphosphate
GG 508:509 (≠ GQ 416:417) binding FAD
GAM 511:513 (≠ GPL 419:421) binding thiamine diphosphate
D538 (= D446) binding Mg(2+)
DGS 538:540 (≠ DYD 446:448) binding thiamine diphosphate
N565 (= N473) binding Mg(2+)
NQHLGM 565:570 (≠ NAYLGL 473:478) binding thiamine diphosphate
H567 (≠ Y475) binding Mg(2+)
W574 (≠ A482) binding chlorimuron-ethyl; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.

Sites not aligning to the query:

653 binding chlorimuron-ethyl; S→A: No effect on catalytic activity or sensitivity to herbicides.; S→F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; S→N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; S→T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.

5k2oA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, pyrithiobac (see paper)
32% identity, 93% coverage: 5:556/593 of query aligns to 13:555/585 of 5k2oA

query
sites
5k2oA

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active site: Y33 (≠ I25), G35 (= G27), G36 (≠ A28), A37 (= A29), S38 (≠ I30), E59 (≠ V51), T82 (= T75), F121 (= F114), Q122 (= Q115), E123 (≠ A116), K171 (≠ F164), M266 (≠ L257), V293 (≠ H285), V400 (≠ I393), G426 (= G419), M428 (≠ L421), D453 (= D446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (≠ L478), V486 (≠ I479), W489 (≠ A482)
binding 2-chloranyl-6-(4,6-dimethoxypyrimidin-2-yl)sulfanyl-benzoic acid: M266 (≠ L257), R292 (= R284), W489 (≠ A482)
binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ C255), G286 (= G278), R288 (= R280), D290 (≠ A282), V293 (≠ H285), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ A322), Q404 (= Q397), M405 (≠ I398), G423 (= G416)
binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ Y475)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (≠ I393), G401 (= G394), Q402 (≠ L395), H403 (≠ S396), M428 (≠ L421), D453 (= D446), G454 (≠ Y447), S455 (≠ D448), N480 (= N473), H482 (≠ Y475), L483 (= L476), G484 (= G477), M485 (≠ L478), V486 (≠ I479)

Sites not aligning to the query:

active site: 558
binding 2-chloranyl-6-(4,6-dimethoxypyrimidin-2-yl)sulfanyl-benzoic acid: 568

Query Sequence

>WP_011914204.1 NCBI__GCF_000013785.1:WP_011914204.1
MARMRAIDAAVAVLRKEGIDTAFGIPGAAINPLYSALRADGGIRHILARHVEGASHMAEG
YTRAKPGNIGVCIGTSGPAGTDMITGLYSASADSIPILCITGQAPRARLHKEDFQAVDIE
SIAKPVTKWAVTVLEPALVPRVFQQAFHVMRSGRPGPVLIDLPFDVQMAEIEFDVDTYEP
LPVYKPAATRKQIEKAIDMLCAAERPLIVAGGGIYNAAAEALLVEFAETVGVPVIPTLMG
WGSIPDDHPLMAGMCGLQTSHRYGNATLLESDFVLGIGNRWANRHTGSVETYTKGRTFVH
VDIEPTQIGRVFAPDYGIVSDARAALELFVDVAKARKAEGRLPDRRAWAADCLERKRTML
RKTNFDSVPMKPQRVYQCMNNAFGKDTCYVSTIGLSQIAAAQFLHVYQPRHWINCGQAGP
LGWTIPAALGVVAADPARKVVALSGDYDFQFMIEELAVGAQFKLPYLHILVNNAYLGLIR
QAQRGFDMDYCVQLGFENINADQSGMEGYGVDHVAVVEGLGCKALRVFKQEDLRPAIEQA
QAWMAEHRVPVVIEVILERVTNIAMGTEIDAINEFEALAAGREDAPTAVGLLD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory